Lin Lin, Jeff DeMartino, Daisong Wang, Gijs J F van Son, Reinier van der Linden, Harry Begthel, Jeroen Korving, Amanda Andersson-Rolf, Stieneke van den Brink, Carmen Lopez-Iglesias, Willine J van de Wetering, Aleksandra Balwierz, Thanasis Margaritis, Marc van de Wetering, Peter J Peters, Jarno Drost, Johan H van Es, Hans Clevers
Enteroendocrine cells (EECs) are hormone-producing cells residing in the epithelium of stomach, small intestine (SI), and colon. EECs regulate aspects of metabolic activity, including insulin levels, satiety, gastrointestinal secretion, and motility. The generation of different EEC lineages is not completely understood. In this work, we report a CRISPR knockout screen of the entire repertoire of transcription factors (TFs) in adult human SI organoids to identify dominant TFs controlling EEC differentiation...
October 27, 2023: Science