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https://www.readbyqxmd.com/read/29032785/two-glutathione-transferase-isoforms-isolated-from-juvenile-cysts-of-taenia-crassiceps-identification-purification-and-characterization
#1
G Maldonado, G Nava, A Plancarte
We identified and characterized the first two glutathione transferases (GSTs) isolated from juvenile cysts of Taenia crassiceps (EC 2.5.1.18). The two glutathione transferases (TcGST1 and TcGST2) were purified in a single-step protocol using glutathione (GSH)-sepharose chromatography in combination with a GSH gradient. The specific activities of TcGST1 and TcGST2 were 26 U mg-1 and 19 U mg-1, respectively, both at 25°C and pH 6.5 with 1-chloro-2,4-dinitrobenzene (CDNB) and GSH as substrates. The K m(CDNB) and K cat(CDNB) values for TcGST1 and TcGST2 (0...
October 16, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/29029769/role-of-imaging-techniques-in-the-diagnosis-and-follow-up-of-muscle-invasive-bladder-carcinoma
#2
A Mesa, E Nava, A Fernández Del Valle, B Argüelles, R Menéndez-Del Llano, S Sal de Rellán
INTRODUCTION: Muscle-invasive bladder malignancies represent 20-30% of all bladder cancers. These patients require imaging tests to determine the regional and distant staging. OBJECTIVE: To describe the role of various imaging tests in the diagnosis, staging and follow-up of muscle-invasive bladder cancer. To assess recent developments in radiology aimed at improving the sensitivity and specificity of local staging and treatment response. ACQUISITION OF EVIDENCE: We conducted an updated literature review...
October 10, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/29018595/assessing-the-living-and-dead-proportions-of-cold-water-coral-colonies-implications-for-deep-water-marine-protected-area-monitoring-in-a-changing-ocean
#3
Johanne Vad, Covadonga Orejas, Juan Moreno-Navas, Helen S Findlay, J Murray Roberts
Coral growth patterns result from an interplay of coral biology and environmental conditions. In this study colony size and proportion of live and dead skeletons in the cold-water coral (CWC) Lophelia pertusa (Linnaeus, 1758) were measured using video footage from Remotely Operated Vehicle (ROV) transects conducted at the inshore Mingulay Reef Complex (MRC) and at the offshore PISCES site (Rockall Bank) in the NE Atlantic. The main goal of this paper was to explore the development of a simple method to quantify coral growth and its potential application as an assessment tool of the health of these remote habitats...
2017: PeerJ
https://www.readbyqxmd.com/read/29016603/characterization-of-hepatitis-b-virus-in-amerindian-children-and-mothers-from-amazonas-state-colombia
#4
Carlos Mario Jaramillo, Fernando de La Hoz, Alexandra Porras, Diana di Filippo, Luz Angela Choconta-Piraquive, Edra Payares, Neyla Montes, Maria-Cristina Navas
BACKGROUND: Hepatitis B Virus (HBV) infection is a worldwide public health problem. In the 1980's a highly effective and safe vaccine against HBV was developed, although breakthrough infection still occasionally occurs because of the emergence of escape mutants. The aim of this study was to identify HBV genotypes and escape mutants in children and their mothers in Amerindian communities of the Amazonas State, Southern Colombia. METHODS: Blood specimens collected from children and mothers belonging to 37 Amerindian communities in Amazonas state, were screened for HBsAg and anti-HBc using ELISA...
2017: PloS One
https://www.readbyqxmd.com/read/28980080/complete-genome-sequence-of-datura-leaf-curl-virus-a-novel-begomovirus-infecting-datura-innoxia-in-sudan-related-to-begomoviruses-causing-tomato-yellow-leaf-curl-disease
#5
Heyam S Mohammed, Marmar A El Siddig, Adil A El Hussein, Jesús Navas-Castillo, Elvira Fiallo-Olivé
Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named "datura leaf curl virus" (DaLCV). Pairwise sequence comparisons and phylogenetic analysis showed that DaLCV isolates are related to begomoviruses causing tomato yellow leaf curl disease, a global menace for tomato crops...
October 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28941716/blood-and-urine-cadmium-concentrations-and-walking-speed-in-middle-aged-and-older-u-s-adults
#6
Junghoon Kim, Esther Garcia-Esquinas, Ana Navas-Acien, Yoon-Hyeong Choi
Reduced physical performance is an important feature of aging, and walking speed is a valid measure of physical performance and mobility in older adults. Previous epidemiological studies suggest that cadmium exposure, even at low environmental levels, may contribute to vascular, musculoskeletal, and cognitive dysfunction, which may all be associated with reductions in physical performance. To this end, we investigated the associations of blood and urine cadmium concentrations with walking speed in middle-aged and older adults in the U...
September 20, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28934725/prenatal-exposure-to-mercury-associations-with-global-dna-methylation-and-hydroxymethylation-in-cord-blood-and-in-childhood
#7
Andres Cardenas, Sheryl L Rifas-Shiman, Lode Godderis, Radu-Corneliu Duca, Ana Navas-Acien, Augusto A Litonjua, Dawn L DeMeo, Kasey J Brennan, Chitra J Amarasiriwardena, Marie-France Hivert, Matthew W Gillman, Emily Oken, Andrea A Baccarelli
BACKGROUND: Mercury is a global pollutant, and prenatal exposure is associated with adverse health effects. To date, no studies have evaluated the association between prenatal mercury exposure and DNA hydroxymethylation, an epigenetic modification important for tissue differentiation and embryonic development. OBJECTIVES: We sought to evaluate the association between prenatal mercury exposure and offspring global DNA methylation and hydroxymethylation at birth and test for persistence of the association in childhood...
August 29, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28906339/multiparametric-magnetic-resonance-imaging-of-prostate-cancer-bone-disease-correlation-with-bone-biopsy-histological-and-molecular-features
#8
Raquel Perez-Lopez, Daniel Nava Rodrigues, Ines Figueiredo, Joaquin Mateo, David J Collins, Dow-Mu Koh, Johann S de Bono, Nina Tunariu
OBJECTIVES: The aim of this study was to correlate magnetic resonance imaging (MRI) of castration-resistant prostate cancer (CRPC) bone metastases with histological and molecular features of bone metastases. MATERIALS AND METHODS: Forty-three bone marrow biopsies from 33 metastatic CRPC (mCRPC) patients with multiparametric MRI and documented bone metastases were evaluated. A second cohort included 10 CRPC patients with no bone metastases. Associations of apparent diffusion coefficient (ADC), normalized b900 diffusion-weighted imaging (nDWI) signal, and signal-weighted fat fraction (swFF) with bone marrow biopsy histological parameters were evaluated using Mann-Whitney U test and Spearman correlations...
September 12, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28898254/serum-levels-of-adiponectin-and-leptin-as-biomarkers-of-proteinuria-in-lupus-nephritis
#9
Valeria Diaz-Rizo, David Bonilla-Lara, Laura Gonzalez-Lopez, Dalia Sanchez-Mosco, Nicte S Fajardo-Robledo, Edsaul E Perez-Guerrero, N Alejandra Rodriguez-Jimenez, A Miriam Saldaña-Cruz, M Luisa Vazquez-Villegas, Eduardo Gomez-Bañuelos, Monica Vazquez-Del Mercado, E German Cardona-Muñoz, David Cardona-Muller, Xochitl Trujillo, Miguel Huerta, Mario Salazar-Paramo, Jorge I Gamez-Nava
INTRODUCTION: There are controversial results about the role of serum leptin and adiponectin levels as biomarkers of the severity of proteinuria in lupus nephritis. OBJECTIVE: The aim of this study was to evaluate the relationship between serum leptin and adiponectin levels with severity of proteinuria secondary to lupus nephritis (LN). METHODS: In a cross-sectional study, 103 women with systemic lupus erythematosus (SLE) were evaluated for kidney involvement...
2017: PloS One
https://www.readbyqxmd.com/read/28893428/correlation-between-preoperative-serum-alpha-fetoprotein-levels-and-survival-with-respect-to-the-surgical-treatment-of-hepatocellular-carcinoma-at-a-tertiary-care-hospital-in-veracruz-mexico
#10
G Martínez-Mier, S Esquivel-Torres, A Nava-Lacorte, F A Lajud-Barquín, S Zilli-Hernández, L M Vázquez-Ramírez
INTRODUCTION: Preoperative serum alpha-fetoprotein levels can have predictive value for hepatocellular carcinoma survival. AIM: Our aim was to analyze the correlation between preoperative serum alpha-fetoprotein levels and survival, following the surgical treatment of hepatocellular carcinoma. METHODS: Nineteen patients were prospectively followed (07/2005-01/2016). An ROC curve was created to determine the sensitivity and specificity of alpha-fetoprotein in relation to survival (Kaplan-Meier)...
September 8, 2017: Revista de Gastroenterología de México
https://www.readbyqxmd.com/read/28888870/efficient-differentiation-of-corynebacterium-striatum-corynebacterium-amycolatum-and-corynebacterium-xerosis-clinical-isolates-by-multiplex-pcr-using-novel-species-specific-primers
#11
Carolina S Santos, Juliana N Ramos, Veronica V Vieira, Carina S Pinheiro, Roberto Meyer, Neuza M Alcantara-Neves, Rommel T Ramos, Artur Silva, Raphael Hirata, Liza Felicori, Carlos Ruiz de Alegría Puig, Jesús Navas, Vasco Azevedo, Ana L Mattos-Guaraldi, Luis G C Pacheco
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
November 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28874795/tailoring-the-thermal-conductivity-of-the-powder-bed-in-electron-beam-melting-ebm-additive-manufacturing
#12
C J Smith, S Tammas-Williams, E Hernandez-Nava, I Todd
Metallic powder bed additive manufacturing is capable of producing complex, functional parts by repeatedly depositing thin layers of powder particles atop of each other whilst selectively melting the corresponding part cross-section into each layer. A weakness with this approach arises when melting overhanging features, which have no prior melted material directly beneath them. This is due to the lower thermal conductivity of the powder relative to solid material, which as a result leads to an accumulation of heat and thus distortion...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28866255/novel-junction-specific-and-quantifiable-in-situ-detection-of-ar-v7-and-its-clinical-correlates-in-metastatic-castration-resistant-prostate-cancer
#13
Yezi Zhu, Adam Sharp, Courtney M Anderson, John L Silberstein, Maritza Taylor, Changxue Lu, Pei Zhao, Angelo M De Marzo, Emmanuel S Antonarakis, Mindy Wang, Xingyong Wu, Yuling Luo, Nan Su, Daniel Nava Rodrigues, Ines Figueiredo, Jonathan Welti, Emily Park, Xiao-Jun Ma, Ilsa Coleman, Colm Morrissey, Stephen R Plymate, Peter S Nelson, Johann S de Bono, Jun Luo
BACKGROUND: Androgen receptor splice variant 7 (AR-V7) has been implicated in resistance to abiraterone and enzalutamide treatment in men with metastatic castration-resistant prostate cancer (mCRPC). Tissue- or cell-based in situ detection of AR-V7, however, has been limited by lack of specificity. OBJECTIVE: To address current limitations in precision measurement of AR-V7 by developing a novel junction-specific AR-V7 RNA in situ hybridization (RISH) assay compatible with automated quantification...
August 30, 2017: European Urology
https://www.readbyqxmd.com/read/28866187/interconnected-porosity-analysis-by-3d-x-ray-microtomography-and-mechanical-behavior-of-biomimetic-organic-inorganic-composite-materials
#14
S Alonso-Sierra, R Velázquez-Castillo, B Millán-Malo, R Nava, L Bucio, A Manzano-Ramírez, H Cid-Luna, E M Rivera-Muñoz
Hydroxyapatite-based materials have been used for dental and biomedical applications. They are commonly studied due to their favorable response presented when used for replacement of bone tissue. Those materials should be porous enough to allow cell penetration, internal tissue growth, vascular incursion and nutrient supply. Furthermore, their morphology should be designed to guide the growth of new bone tissue in anatomically applicable ways. In this work, the mechanical performance and 3D X-ray microtomography (X-ray μCT) study of a biomimetic, organic-inorganic composite material, based on hydroxyapatite, with physicochemical, structural, morphological and mechanical properties very similar to those of natural bone tissue is reported...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28860265/official-ers-ats-clinical-practice-guidelines-noninvasive-ventilation-for-acute-respiratory-failure
#15
Bram Rochwerg, Laurent Brochard, Mark W Elliott, Dean Hess, Nicholas S Hill, Stefano Nava, Paolo Navalesi, Massimo Antonelli, Jan Brozek, Giorgio Conti, Miquel Ferrer, Kalpalatha Guntupalli, Samir Jaber, Sean Keenan, Jordi Mancebo, Sangeeta Mehta, Suhail Raoof
Noninvasive mechanical ventilation (NIV) is widely used in the acute care setting for acute respiratory failure (ARF) across a variety of aetiologies. This document provides European Respiratory Society/American Thoracic Society recommendations for the clinical application of NIV based on the most current literature.The guideline committee was composed of clinicians, methodologists and experts in the field of NIV. The committee developed recommendations based on the GRADE (Grading, Recommendation, Assessment, Development and Evaluation) methodology for each actionable question...
August 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28852987/drosophila-suzukii-in-southern-neotropical-region-current-status-and-future-perspectives
#16
REVIEW
F Andreazza, D Bernardi, R S S Dos Santos, F R M Garcia, E E Oliveira, M Botton, D E Nava
Non-native insect pests are often responsible for important damage to native and agricultural plant hosts. Since Drosophila suzukii Matsumura (Diptera: Drosophilidae) has become an important pest in North America and Europe (i.e., in 2008), the global production of soft thin-skinned fruits has faced severe production losses. In the southern Neotropical region, however, the first record of D. suzukii occurred in 2013 in the south of Brazil. It has also been recorded in Uruguay, Argentina, and Chile. Despite its recent occurrence in the southern Neotropical region, the fast dispersion of D...
August 30, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28826504/development-of-bag-1l-as-a-therapeutic-target-in-androgen-receptor-dependent-prostate-cancer
#17
Laura Cato, Antje Neeb, Adam Sharp, Victor Buzón, Scott B Ficarro, Linxiao Yang, Claudia Muhle-Goll, Nane C Kuznik, Ruth Riisnaes, Daniel Nava Rodrigues, Olivier Armant, Victor Gourain, Guillaume Adelmant, Emmanuel A Ntim, Thomas Westerling, David Dolling, Pasquale Rescigno, Ines Figueiredo, Friedrich Fauser, Jennifer Wu, Jaice T Rottenberg, Liubov Shatkina, Claudia Ester, Burkhard Luy, Holger Puchta, Jakob Troppmair, Nicole Jung, Stefan Bräse, Uwe Strähle, Jarrod A Marto, Gerd Ulrich Nienhaus, Bissan Al-Lazikani, Xavier Salvatella, Johann S de Bono, Andrew Cb Cato, Myles Brown
Targeting the activation function-1 (AF-1) domain located in the N-terminus of the androgen receptor (AR) is an attractive therapeutic alternative to the current approaches to inhibit AR action in prostate cancer (PCa). Here we show that the AR AF-1 is bound by the cochaperone Bag-1L. Mutations in the AR interaction domain or loss of Bag-1L abrogate AR signaling and reduce PCa growth. Clinically, Bag-1L protein levels increase with progression to castration-resistant PCa (CRPC) and high levels of Bag-1L in primary PCa associate with a reduced clinical benefit from abiraterone when these tumors progress...
August 10, 2017: ELife
https://www.readbyqxmd.com/read/28798383/novel-giardicidal-compounds-bearing-proton-pump-inhibitor-scaffold-proceeding-through-triosephosphate-isomerase-inactivation
#18
B Hernández-Ochoa, G Navarrete-Vázquez, C Nava-Zuazo, A Castillo-Villanueva, S T Méndez, A Torres-Arroyo, S Gómez-Manzo, J Marcial-Quino, M Ponce-Macotela, Y Rufino-González, M Martínez-Gordillo, G Palencia-Hernández, N Esturau-Escofet, E Calderon-Jaimes, J Oria-Hernández, H Reyes-Vivas
Giardiasis is a worldwide parasitic disease that affects mainly children and immunosuppressed people. Side effects and the emergence of resistance over current used drugs make imperative looking for new antiparasitics through discovering of new biological targets and designing of novel drugs. Recently, it has determined that gastric proton-pump inhibitors (PPI) have anti-giardiasic activity. The glycolytic enzyme, triosephosphate isomerase (GlTIM), is one of its potential targets. Therefore, we employed the scaffold of PPI to design new compounds aimed to increase their antigiardial capacity by inactivating GlTIM...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28758134/polymorphism-rs2073618-of-the-tnfrsf11b-opg-gene-and-bone-mineral-density-in-mexican-women-with-rheumatoid-arthritis
#19
C A Nava-Valdivia, A M Saldaña-Cruz, E G Corona-Sanchez, J D Murillo-Vazquez, M C Moran-Moguel, M Salazar-Paramo, E E Perez-Guerrero, M L Vazquez-Villegas, D Bonilla-Lara, A D Rocha-Muñoz, B T Martín-Marquez, F Sandoval-Garcia, E A Martínez-García, N S Fajardo-Robledo, J M Ponce-Guarneros, M Ramirez-Villafaña, M F Alcaraz-Lopez, L Gonzalez-Lopez, J I Gamez-Nava
Osteoporosis (OP) is highly prevalent in rheumatoid arthritis (RA) and is influenced by genetic factors. Single-nucleotide polymorphism (SNP) rs2073618 in the TNFRSF11B osteoprotegerin (OPG) gene has been related to postmenopausal OP although, to date, no information has been described concerning whether this polymorphism is implied in abnormalities of bone mineral density (BMD) in RA. We evaluated, in a case-control study performed in Mexican-Mestizo women with RA, whether SNP rs2073618 in the TNFRSF11B gene is associated with a decrease in BMD...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28756866/tuberculosis-granulomatosis-with-polyangiitis-or-both-a-case-report
#20
Á Nava-Castañeda, F Martín, S Voorduin, F Zuazo
INTRODUCTION: Tuberculosis (TB) is a chronic granulomatose infection, and granulomatosis with polyangiitis (GP) is a small vessel vasculitis, both of which affect the lungs. The combination of these diseases is rare. Both have similar clinical features, making the differential diagnosis difficult. CASE REPORT: It concerns a 37 year-old female undergoing treatment for pulmonary TB, who presented with left ocular proptosis, eyelid and conjunctival edema and erythema...
July 27, 2017: Archivos de la Sociedad Española de Oftalmología
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