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Poly I:C

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https://www.readbyqxmd.com/read/29018123/characterization-of-inner-and-outer-membrane-proteins-from-francisella-tularensis-strains-lvs-and-schu-s4-and-identification-of-potential-subunit-vaccine-candidates
#1
Deborah M B Post, Bram Slütter, Birgit Schilling, Aroon T Chande, Jed A Rasmussen, Bradley D Jones, Alexandria K D'Souza, Lorri M Reinders, John T Harty, Bradford W Gibson, Michael A Apicella
Francisella tularensis is the causative agent of tularemia and a potential bioterrorism agent. In the present study, we isolated, identified, and quantified the proteins present in the membranes of the virulent type A strain, Schu S4, and the attenuated type B strain, LVS (live vaccine strain). Spectral counting of mass spectrometric data showed enrichment for membrane proteins in both strains. Mice vaccinated with whole LVS membranes encapsulated in poly (lactic-co-glycolic acid) (PLGA) nanoparticles containing the adjuvant polyinosinic-polycytidylic acid [poly(I·C)] showed significant protection against a challenge with LVS compared to the results seen with naive mice or mice vaccinated with either membranes or poly(I·C) alone...
October 10, 2017: MBio
https://www.readbyqxmd.com/read/29018080/chd7-deficiency-delays-leukemogenesis-in-mice-induced-by-cbfb-myh11
#2
Tao Zhen, Erika Kwon, Ling Zhao, Jingmei Hsu, R Katherine Hyde, Ying Lu, Lemlem Alemu, Nancy A Speck, P Paul Liu
Inversion of chromosome 16 is a consistent finding in patients with acute myeloid leukemia subtype M4 with eosinophilia (AML M4Eo), which generates a CBFB-MYH11 fusion gene. Previous studies showed that the interaction between CBFβ-SMMHC (encoded by CBFB-MYH11) and RUNX1 plays a critical role in the pathogenesis of this leukemia. Recently, it was shown that chromodomain-helicase-DNA binding protein 7 (CHD7) interacts with RUNX1 and suppresses RUNX1-induced expansion of hematopoietic stem and progenitor cells...
October 10, 2017: Blood
https://www.readbyqxmd.com/read/29017949/tumor-necrosis-factor-receptor-associated-factor-3-from-anodonta-woodiana-is-an-important-factor-in-bivalve-immune-response-to-pathogen-infection
#3
Fufa Qu, Zhiming Xiang, Yingli Zhou, Yanping Qin, Ziniu Yu
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a multifunctional adaptor protein in innate and acquired immune system that plays a key role in the regulation of the RIG-I-like receptor (RLR) and Toll-like receptor (TLR) signaling pathway in mammals. However, the immune function of TRAF3 homologs in freshwater mollusks is not well understood. In this study, we identified a bivalve TRAF3 gene (AwTRAF3) from Anodonta woodiana and investigated its potential roles during immune challenges. The present AwTRAF3 encoded a polypeptide of 562 amino acids with predicted molecular mass of 64...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29017948/transcriptome-analysis-of-hepatopancraes-of-procambarus-clarkii-challenged-with-polyriboinosinic-polyribocytidylic-acid-poly-i-c
#4
Li-Shang Dai, Muhammad Nadeem Abbas, Saima Kausar, Yang Zhou
Crustacean hepatopancreas regulates metabolic processes, biogenesis and innate immune processes, and the knowledge on its immune genes are crucial to understand antimicrobial mechanisms. In this study, we reported the transcriptomic profile of Procambarus clarkii hepatopancreas after poly I:C administration using high-throughput sequencing. Following de novo assembly 56,716 unigene sequences with an average length of 810 bp was obtained. The unigene sequences were annotated to three ontologies including cellular components, biological processes and molecular functions, further 56,716 unigene sequences were mapped to 25 COG categories...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29017946/cloning-and-expression-of-malabar-grouper-epinephelus-malabaricus-adar1-gene-in-response-to-immune-stimulants-and-nervous-necrosis-virus
#5
Thirunavukkarasu Periyasamy, Joan Tang Xiao Joe, Ming-Wei Lu
ADARs are RNA editing catalysts that bind double-stranded RNA and convert adenosine to inosine, a process that can lead to destabilization of dsRNA structures and suppression of mRNA translation. In mammals, ADAR1 genes are involved in various cellular pathways, including interferon (IFN)-mediated response. However, the function of fish ADAR1 remains unclear. We report here the cloning of ADAR1 in Malabar grouper (Epinephelus malabaricus) (MgADAR1) and its response to various immune stimulants. The MgADAR1 cDNA is 5371-bp long, consisting of an open reading frame encoding a putative protein of 1381 amino acids, a 235-nt 5'-terminal untranslated region (UTR), and a 990-nt 3'-UTR...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29017941/novel-ifn%C3%AE-homologue-identified-in-nile-tilapia-oreochromis-niloticus-links-with-immune-response-in-gills-under-different-stimuli
#6
Janet Velázquez, Jannel Acosta, Naylin Herrera, Antonio Morales, Osmany González, Fidel Herrera, Mario Pablo Estrada, Yamila Carpio
Interferon gamma (IFN-γ) has important roles in both innate and adaptive immune responses. This cytokine plays a very important role in defining Th1 immune response in all vertebrates. In the present study, we identified and isolated for the first time the gene coding for Nile tilapia (Oreochromis niloticus) IFNγ from spleen lymphocytes. The isolated tilapia IFNγ has between 23 and 62% of amino acid identity as compared to reported sequences for other teleost fishes. It has close phylogenetic relationships with IFNγ molecules belonging to the group of Perciforms and presents the typical structural characteristics of gamma interferon molecules...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28993949/poly-n-methylated-a%C3%AE-peptide-c-terminal-fragments-meptides-reverse-the-deleterious-effects-of-amyloid-%C3%AE-in-rats
#7
Siya G Sibiya, Musa V Mbandla, Thavi Govender, Adeola Shobo, William M U Daniels
Alzheimer's disease (AD) is characterized by extracellular deposition of amyloid-β (Aβ) plaques. These protein deposits impair synaptic plasticity thereby producing a progressive decline in cognitive function. Current therapies are merely palliative and only slow cognitive decline. Poly-N-methylated Aβ-Peptide C-Terminal Fragments (MEPTIDES) were recently shown to reduce Aβ toxicity in vitro and in Drosophila melanogaster, however whether these novel compounds are effective in inhibiting Aβ-induced toxicity in the mammalian brain remains unclear...
October 9, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28990627/controlling-the-generation-of-bilayer-and-multilayer-vesicles-in-block-copolymer-epoxy-blends-by-a-slow-photopolymerization-process
#8
J Puig, M Ceolín, R J J Williams, W F Schroeder, I A Zucchi
Vesicles are a highly attractive morphology to achieve in micellar dispersions of block copolymers (BCP) in epoxy thermosets due to the fact that small amounts can affect a large volume fraction of the matrix, a fact that is important for toughening purposes. However, generating vesicles in epoxy matrices requires operating in a narrow range of formulations and processing conditions. In this report, we show that block-copolymer vesicles dispersed in an epoxy matrix could be obtained through a sphere-to-cylinder-to-vesicle micellar transition induced by visible-light photopolymerization at room temperature...
October 9, 2017: Soft Matter
https://www.readbyqxmd.com/read/28986216/innate-immune-responses-against-rock-bream-iridovirus-rbiv-infection-in-rock-bream-oplegnathus-fasciatus-following-poly-i-c-administration
#9
Myung-Hwa Jung, Sung-Ju Jung
Poly (I:C) showed promise as an immunoprotective agents in rock bream against rock bream iridovirus (RBIV) infection. In this study, we evaluated the time-dependent virus replication pattern and antiviral immune responses in RBIV-infected rock bream with and without poly (I:C) administration. In the poly (I:C)+virus-injected group, virus copy numbers were more than 18.9-, 24.0- and 479.2-fold lower than in the virus only injected group at 4 (4.73 × 10(4) and 8.95 × 10(5)/μl, respectively), 7 (3.67 × 10(5) and 8...
October 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28984294/perinatal-immune-activation-produces-persistent-sleep-alterations-and-epileptiform-activity-in-male-mice
#10
Galen Missig, Emery L Mokler, James O Robbins, Abigail J Alexander, Christopher J McDougle, William A Carlezon
Increasing evidence suggests a role for inflammation in neuropsychiatric conditions including autism spectrum disorder (ASD). Previous work in rodents has established that immune activation during critical developmental periods can cause phenotypes that reproduce core features of ASD, including decreased social interaction, aberrant communication, and increased repetitive behavior. In humans, ASD is frequently associated with co-morbid medical conditions including sleep disorders, motor hyperactivity, and seizures...
October 6, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28983294/lipoteichoic-acid-of-probiotic-lactobacillus-plantarum-attenuates-poly-i-c-induced-il-8-production-in-porcine-intestinal-epithelial-cells
#11
Kyoung Whun Kim, Seok-Seong Kang, Sun-Je Woo, Ok-Jin Park, Ki Bum Ahn, Ki-Duk Song, Hak-Kyo Lee, Cheol-Heui Yun, Seung Hyun Han
Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic functions in livestock. The present study demonstrated that lipoteichoic acid (LTA) of Lactobacillus plantarum (Lp.LTA) confers anti-inflammatory responses in porcine intestinal epithelial cell line, IPEC-J2. A synthetic analog of viral double-stranded RNA, poly I:C, dose-dependently induced IL-8 production at the mRNA and protein levels in IPEC-J2 cells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28978569/marginal-zone-b-cells-are-critical-to-factor-viii-inhibitor-formation-in-mice-with-hemophilia-a
#12
Patricia E Zerra, Courtney Cox, W Hunter Baldwin, Seema R Patel, Connie M Arthur, Pete Lollar, Shannon L Meeks, Sean R Stowell
While factor VIII (FVIII) replacement therapy can be life saving for patients with hemophilia A, neutralizing alloantibodies to FVIII, known as inhibitors, develop in a significant number of patients and actively block FVIII activity, making bleeding difficult to control and prevent. Although a variety of downstream immune factors likely regulate inhibitor formation, the identification and subsequent targeting of key initiators in inhibitor development may provide an attractive approach to prevent inhibitor formation before amplification of the FVIII immune response occurs...
October 4, 2017: Blood
https://www.readbyqxmd.com/read/28977843/orphan-nuclear-receptor-nur77-inhibits-poly-i-c-triggered-acute-liver-inflammation-by-inducing-the-ubiquitin-editing-enzyme-a20
#13
Xiu-Ming Li, Tian-Yu Yang, Xiao-Shun He, Jing-Ru Wang, Wen-Juan Gan, Shen Zhang, Jian-Ming Li, Hua Wu
Inflammation is a key contributor to various types of acute and chronic liver disease. We recently reported that lack of Nur77, an orphan nuclear receptor, contributes to the pathogenesis of inflammatory diseases including inflammatory bowel disease and sepsis. However, whether Nur77 plays a critical role in liver inflammation remains to be fully understood. Employing in vivo acute liver inflammation model in wild-type (Nur77(+/+)) and Nur77(-/-) mice, we here found that Nur77 deficiency dramatically increased the production of pro-inflammatory cytokines and accelerated liver injury induced by poly (I:C)/D-GalN in Nur77(-/-) mice...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28976507/two-step-volume-phase-transition-mechanism-of-poly-n-vinylcaprolactam-hydrogel-online-tracked-by-two-dimensional-correlation-spectroscopy
#14
Gehong Su, Tao Zhou, Xifei Liu, Yulin Zhang
In this study, temperature-dependent FTIR spectroscopy in combination with the perturbation-correlation moving-window (PCMW2D) technique and generalized two-dimensional (2D) correlation analysis was applied to investigate the phase transition mechanism of poly(N-vinylcaprolactam) (PVCL) hydrogel upon heating. In the conventional 1D FTIR spectra, the gradual dehydration of C-H groups, as well as the gradual dissociation of hydrogen bonds between C[double bond, length as m-dash]O groups and water molecules, was observed during phase transition...
October 4, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28975372/t-buooh-induces-ferroptosis-in-human-and-murine-cell-lines
#15
Christine Wenz, Dagmar Faust, Berenike Linz, Christian Turmann, Teodora Nikolova, John Bertin, Peter Gough, Peter Wipf, Anna Sophia Schröder, Stefan Krautwald, Cornelia Dietrich
Reactive oxygen species (ROS)-induced apoptosis has been extensively studied. Increasing evidence suggests that ROS, for instance, induced by hydrogen peroxide (H2O2), might also trigger regulated necrotic cell death pathways. Almost nothing is known about the cell death pathways triggered by tertiary-butyl hydroperoxide (t-BuOOH), a widely used inducer of oxidative stress. The lipid peroxidation products induced by t-BuOOH are involved in the pathophysiology of many diseases, such as cancer, cardiovascular diseases, or diabetes...
October 3, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28974092/poly-i-c-encapsulating-nanoparticles-enhance-innate-immune-responses-to-the-tuberculosis-vaccine-bacille-calmette-gu%C3%A3-rin-bcg-via-synergistic-activation-of-innate-immune-receptors
#16
Martin T Speth, Urska Repnik, Elisabeth Müller, Julia Spanier, Ulrich Kalinke, Alexandre Corthay, Gareth Griffiths
The attenuated live vaccine strain Bacille Calmette-Guérin (BCG) is currently the only available vaccine against tuberculosis (TB), but is largely ineffective against adult pulmonary TB, the most common disease form. This is in part due to BCG's ability to interfere with the host innate immune response, a feature that might be targeted to enhance the potency of this vaccine. Here, we investigated the ability of chitosan-based nanoparticles (pIC-NPs) containing polyinosinic-polycytidylic acid (poly(I:C)), an inducer of innate immunity via Toll-like receptor 3 (TLR3) to enhance the immunogenicity of BCG in mouse bone marrow-derived macrophages (BMDM) in vitro...
October 3, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28970832/use-of-toll-like-receptor-agonists-to-induce-ectopic-lymphoid-structures-in-myasthenia-gravis-mouse-models
#17
Marieke Robinet, Bérengère Villeret, Solène Maillard, Mélanie A Cron, Sonia Berrih-Aknin, Rozen Le Panse
Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies against the acetylcholine receptor (AChR) at the neuromuscular junction. MG symptoms are characterized by muscle weaknesses. The thymus of MG patients is very often abnormal and possesses all the characteristics of tertiary lymphoid organs such as neoangiogenesis processes, overexpression of inflammatory cytokines and chemokines, and infiltration of B lymphocytes leading to ectopic germinal center (GC) development. We previously demonstrated that injections of mice with polyinosinic-polycytidylic acid [Poly(I:C)], a synthetic double-stranded RNA mimicking viral infection, induce thymic changes and trigger MG symptoms...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28970247/chromatin-remodeling-demethylating-h3k4me3-of-type-i-ifns-gene-by-rbp2-through-interacting-with-piasy-for-transcriptional-attenuation
#18
Xiaoli Yu, Hui Chen, Chen Zuo, Xi Jin, Yibing Yin, Hong Wang, Mei Jin, Keiko Ozato, Songxiao Xu
Type I IFNs (IFNIs) are involved in the course of antiviral and antimicrobial activities; however, robust inductions of these can lead to host immunopathology. We have reported that the Pias (protein inhibitor of activated signal transducer and activator of transcription) family member, Piasy, possesses the ability to suppress IFNI transcriptions in mouse embryonic fibroblasts (MEFs), yet the specific molecular mechanism by which it acts remains elusive. Here, we identify that the H3K4me3 levels, one activation mark of genes, in MEFs that were stimulated by poly(I:C) were impaired by Piasy in the IFN-β gene...
September 25, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28970238/hcfc2-is-needed-for-irf1-and-irf2-dependent-tlr3-transcription-and-for-survival-during-viral-infections
#19
Lei Sun, Zhengfan Jiang, Victoria A Acosta-Rodriguez, Michael Berger, Xin Du, Jin Huk Choi, Jianhui Wang, Kuan-Wen Wang, Gokhul K Kilaru, Jennifer A Mohawk, Jiexia Quan, Lindsay Scott, Sara Hildebrand, Xiaohong Li, Miao Tang, Xiaoming Zhan, Anne R Murray, Diantha La Vine, Eva Marie Y Moresco, Joseph S Takahashi, Bruce Beutler
Transcriptional regulation of numerous interferon-regulated genes, including Toll-like receptor 3 (Tlr3), which encodes an innate immune sensor of viral double-stranded RNA, depends on the interferon regulatory factor 1 (IRF1) and IRF2 transcription factors. We detected specific abrogation of macrophage responses to polyinosinic-polycytidylic acid (poly(I:C)) resulting from three independent N-ethyl-N-nitrosourea-induced mutations in host cell factor C2 (Hcfc2). Hcfc2 mutations compromised survival during influenza virus and herpes simplex virus 1 infections...
October 2, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28969942/duck-stimulator-of-interferon-genes-plays-an-important-role-in-host-anti-duck-plague-virus-infection-through-an-ifn-dependent-signalling-pathway
#20
Shun Chen, Zhen Wu, Jinyue Zhang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xinxin Zhao, Anchun Cheng
The human stimulator of interferon gene (STING) is an important molecule in innate immunity that stimulates type I interferon (IFN) production. However, the role of duck STING (duSTING) in innate immunity has yet to be explained. In this study, the full length of the duSTING cDNA sequence (1149bp), which encodes 382 amino acid (aa) residues, was reported and showed the highest sequence similarity with chicken STINGs. The phylogenetic analysis based on STING aa showed that duSTING was grouped onto the birds clade...
September 30, 2017: Cytokine
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