keyword
MENU ▼
Read by QxMD icon Read
search

Poly I:C

keyword
https://www.readbyqxmd.com/read/28635367/in-vivo-and-in-vitro-depolymerizations-of-intracellular-medium-chain-length-poly-3-hydroxyalkanoates-produced-by-pseudomonas-putida-bet001
#1
Siti Nor Syairah Anis, Mohamad Suffian Mohamad Annuar, Khanom Simarani
In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 grown on lauric acid was studied. Both processes were studied under optimum conditions for mcl-PHA depolymerization viz. 0.2 M Tris-HCl buffer, pH 9, ionic strength (I) = 0.2 M at 30 °C. For in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 kHz, 30% of power output, <25 (°)C for 120 minutes)...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28633526/reduced-graphene-oxide-poly-acrylic-acid-aerogels-as-a-three-dimensional-replacement-for-metal-foil-current-collectors-in-lithium-ion-batteries
#2
Han Xiao, Joshua P Pender, Mackenzie Meece-Rayle, J Pedro de Souza, Kyle C Klavetter, Heonjoo Ha, Jie Lin, Adam Heller, Christopher J Ellison, Charles Buddie Mullins
We report the synthesis and properties of a low-density (~5 mg/cm3) and highly-porous (99.6% void space) three-dimensional reduced graphene oxide (rGO)/poly(acrylic acid) (PAA) nanocomposite aerogel as the scaffold for cathode materials in lithium-ion batteries (LIBs). The rGO-PAA is both simple and starts from readily-available graphite and PAA thereby providing a scalable fabrication procedure. The scaffold can support as much as a 75 mg/cm2 loading of LiFePO4 (LFP) in a ~ 430 µm thick layer, and the porosity of the aerogel is tunable by compression; the flexible aerogel can be compressed 30-fold (i...
June 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28630015/characterization-of-japanese-flounder-paralichthys-olivaceus-caspase1-involved-in-extracellular-atp-mediated-immune-signaling-in-fish
#3
Shuo Li, Weijiao Peng, Jiafang Li, Gaixiang Hao, Xuyun Geng, Jinsheng Sun
Caspase1 is a member of inflammatory Caspases that play important roles in the innate immune system. Although several teleost caspase1 genes have been identified, their partner proteins and implication in extracellular ATP-mediated immune signaling in fish are still very limited. Here we identified and characterized a caspase1 gene, named JfCaspase1, from Japanese flounder Paralichthys olivaceus. JfCaspase1 mRNA was constitutively expressed in all examined normal tissues with high expression in skin and gills and moderate expression in the enriched Japanese flounder head kidney macrophages (HKMs) and peripheral blood leukocytes (PBLs)...
June 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#4
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28628008/basophil-histamine-release-to-amoxicilloyl-poly-l-lysine-compared-to-amoxicillin-in-patients-with-ige-mediated-allergic-reactions-to-amoxicillin
#5
F Arribas, S Falkencrone, J Sola, M P Gomez-Serranillos, J J Laguna, M I Montañez, T D Fernandez, D Rodríguez, F Pineda, P Stahl Skov, C Mayorga, M J Torres
BACKGROUND: Amoxicillin (AX) is the betalactam most often involved in IgE-mediated reactions and the diagnosis is mainly based on skin testing (ST) although without optimal sensitivity. We have produced a newly AX derivative, amoxicilloyl-poly-L-lysine (APL), and have analysed its IgE recognition by passive histamine release test (pHRT). METHODS: Patients (N=19) with confirmed AX allergy and with specific IgE positive to AX and controls (N=10) with good tolerance to AX were included...
June 19, 2017: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/28619586/determination-of-acidity-constants-and-prediction-of-electrophoretic-separation-of-amyloid-beta-peptides
#6
Roger Peró-Gascón, Fernando Benavente, José Barbosa, Victoria Sanz-Nebot
In this paper we describe a strategy to estimate by CE the acidity constants (pKa) of complex polyprotic peptides from their building peptide fragments. CE has been used for the determination of the pKas of five short polyprotic peptides that cover all the sequence of amyloid beta (Aβ) peptides 1-40 and 1-42 (Aβ fragments 1-15, 10-20, 20-29, 25-35 and 33-42). First, the electrophoretic mobility (me) was measured as a function of pH of the background electrolyte (BGE) in the pH range 2-12 (bare fused silica capillary, I=25mM and T=25°C)...
June 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28610718/echinacea-purpurea-derived-alkylamides-exhibit-potent-anti-inflammatory-effects-and-alleviate-clinical-symptoms-of-atopic-eczema
#7
Attila Oláh, Judit Szabó-Papp, Michael Soeberdt, Ulrich Knie, Stephan Dähnhardt-Pfeiffer, Christoph Abels, Tamás Bíró
BACKGROUND: Atopic eczema (AE) is a chronic inflammatory and pruritic skin disease. There is still an unmet need for topical anti-inflammatory and anti-pruritic substances exhibiting an excellent safety profile. The endocannabinoid system is known to regulate various aspects of cutaneous barrier and immune functions, thus targeting it may be a valid approach for alleviating the symptoms of AE. OBJECTIVE: To assess the putative efficacy of Echinacea purpurea-derived alkylamides (Ec...
May 27, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28610429/optimization-of-electrospun-poly-n-isopropyl-acrylamide-mats-for-the-rapid-reversible-adhesion-of-mammalian-cells
#8
Kirsten N Cicotte, Jamie A Reed, Phuong Anh H Nguyen, Jacqueline A De Lora, Elizabeth L Hedberg-Dirk, Heather E Canavan
Poly(N-isopropyl acrylamide) (pNIPAM) is a "smart" polymer that responds to changes in altering temperature near physiologically relevant temperatures, changing its relative hydrophobicity. Mammalian cells attach to pNIPAM at 37 °C and detach spontaneously as a confluent sheet when the temperature is shifted below the lower critical solution temperature (∼32 °C). A variety of methods have been used to create pNIPAM films, including plasma polymerization, self-assembled monolayers, and electron beam ionization...
June 13, 2017: Biointerphases
https://www.readbyqxmd.com/read/28609941/mitochondrial-genome-of-i-abraxas-i-i-suspecta-i-lepidoptera-geometridae-%C3%A2-and-comparative-analysis-with-other-lepidopterans
#9
Y U Sun, Jiawei Zhang, Qingqing Li, Dan Liang, Muhammad Nadeem Abbas, Cen Qian, Lei Wang, Guoqing Wei, Bao-Jian Zhu, Chao-Liang Liu
In this study, a complete mitochondrial genome (mitogenome) sequence of Abraxas suspecta (Lepidoptera: Geometridae) is isolated and characterized. The complete DNA is 15,547 bp length and contains 2 ribosomal RNA genes, 23 putative transfer RNA (tRNA) genes including an extra tRNAAsn (AUU), 13 protein-coding genes and an adenine (A) + thymine (T)-rich region. The nucleotide composition and gene organization are identical to those of other lepidopteran, except for the presence of an extra copy of trnN (AUU)...
April 20, 2017: Zootaxa
https://www.readbyqxmd.com/read/28608804/phytosterols-and-omega-3-supplementation-exert-novel-regulatory-effects-on-metabolic-and-inflammatory-pathways-a-proteomic-study
#10
Carmen Lambert Judit Cubedo, Teresa Padró, Joan Sánchez-Hernández, Rosa M Antonijoan, Antonio Perez, Lina Badimon
Cardiovascular disease (CVD) remains one of the major causes of death and disability worldwide. In addition to drug treatment, nutritional interventions or supplementations are becoming a health strategy for CVD prevention. Phytosterols (PhyS) are natural components that have been shown to reduce cholesterol levels; while poly-unsaturated fatty acids (PUFA), mainly omega-3 (ω3) fatty acids, have shown to reduce triglyceride levels. Here we aimed to investigate whether the proteins in the main lipoproteins (low density lipoproteins (LDL) and high density lipoproteins (HDL)) as well as proteins in the lipid free plasma fraction (LPDP) were regulated by the intake of PhyS-milk or ω3-milk, in overweight healthy volunteers by a proteomic based systems biology approach...
June 13, 2017: Nutrients
https://www.readbyqxmd.com/read/28608237/exogenous-adipokine-peptide-resistin-protects-against-focal-cerebral-ischemia-reperfusion-injury-in-mice
#11
Jiangtao Zhu, Di Wu, Chenyu Zhao, Man Luo, Ronald C Hamdy, Balvin H L Chua, Xingshun Xu, Zhigang Miao
Previous studies have demonstrated that plasma resistin levels were increased in patients with acute ischemic stroke. However, the role of resistin after ischemic brain injury is still unclear. In this study, we investigated the protective effects of resistin on cerebral ischemia/reperfusion injury in a middle cerebral artery occlusion mouse model. We found that resistin (i.c.v.) significantly reduced infarct volume and improved neurological deficits after 45 min of ischemia and 24 h of reperfusion. Furthermore, our data demonstrate that intraperitoneal administration of resistin (10 µg/kg body weight) also had protective effects on infarct volume, indicating the crossing of resistin through the impaired BBB after ischemia injury...
June 12, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28603092/crosstalk-of-liver-immune-cells-and-cell-death-mechanisms-in-different-murine-models-of-liver-injury-and-its-clinical-relevance
#12
Hilal Ahmad Khan, Muhammad Zishan Ahmad, Junaid Ali Khan, Muhammad Imran Arshad
BACKGROUND: Liver inflammation or hepatitis is a result of pluripotent interactions of cell death molecules, cytokines, chemokines and the resident immune cells collectively called as microenvironment. The interplay of these inflammatory mediators and switching of immune responses during hepatotoxic, viral, drug-induced and immune cell-mediated hepatitis decide the fate of liver pathology. The present review aimed to describe the mechanisms of liver injury, its relevance to human liver pathology and insights for the future therapeutic interventions...
June 2017: Hepatobiliary & Pancreatic Diseases International: HBPD INT
https://www.readbyqxmd.com/read/28602740/protective-immunity-against-rock-bream-iridovirus-rbiv-infection-and-tlr3-mediated-type-i-interferon-signaling-pathway-in-rock-bream-oplegnathus-fasciatus-following-poly-i-c-administration
#13
Myung-Hwa Jung, Sung-Ju Jung
In this study, we evaluated the potential of poly (I:C) to induce antiviral status for protecting rock bream from RBIV infection. Rock bream injected with poly (I:C) at 2 days before infection (1.1 × 10(4)) at 20 °C had significantly higher protection with RPS 13.4% and 33.4% at 100 and 200 μg/fish, respectively, through 100 days post infection (dpi). The addition of boost immunization with poly (I:C) at before/post infection at 20 °C clearly enhanced the level of protection showing 33.4% and 60.0% at 100 and 200 μg/fish, respectively...
June 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602683/a-c-type-lectin-nattectin-like-protein-cantc-in-qihe-crucian-carp-carassius-auratus-binding-ability-with-lps-pgn-and-various-bacteria-and-agglutinating-activity-against-bacteria
#14
Li Wang, Jie Zhang, Xianghui Kong, Xianliang Zhao, Chao Pei, Li Li
C-type lectins (CTLs), as the members of pattern-recognition receptors (PRRs), play the significant roles in innate immunity through binding with pathogen-associated molecular patterns (PAMPs) on the surface of microbe. In the present study, a novel CTL, Nattectin-like protein (named as CaNTC), was investigated in Qihe crucian carp Carassius auratus. The full-length cDNA of CaNTC was composed of 776 bp, with a 152 bp 5'-untranslated region (UTR), a 492 bp ORF encoding a 163-aa protein, and a 132 bp 3'-UTR with a polyadenylation signal sequence AATAAA and a poly(A) tail...
June 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28602679/characterization-of-the-black-carp-traf6-signaling-molecule-in-innate-immune-defense
#15
Shu Jiang, Jun Xiao, Jun Li, Hui Chen, Chanyuan Wang, Chaoliang Feng, Hao Feng
Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a vital role in the innate immune response of higher vertebrates. To elucidate its function in teleost fish, TRAF6 homologue of black carp (Mylopharyngodon piceus) has been cloned and characterized in this study. Black carp TRAF6 (bcTRAF6) transcription in Mylopharyngodon piceus fin (MPF) cells was up-regulated in response to both poly (I:C) treatment and viral infection, but was suppressed by LPS stimulation. bcTRAF6 migrated around 72 KDa in immunoblot analysis and was identified as a cytosolic protein suggested to be associated with vesicles scattering in the cytoplasm...
June 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28601925/enhanced-stimulation-of-human-tumor-specific-t-cells-by-dendritic-cells-matured-in-the-presence-of-interferon-%C3%AE-and-multiple-toll-like-receptor-agonists
#16
Tanja Lövgren, Dhifaf Sarhan, Iva Truxová, Bhavesh Choudhary, Roeltje Maas, Jeroen Melief, Maria Nyström, Ulrika Edbäck, Renee Vermeij, Gina Scurti, Michael Nishimura, Giuseppe Masucci, Alex Karlsson-Parra, Andreas Lundqvist, Lars Adamson, Rolf Kiessling
Dendritic cell (DC) vaccines have been demonstrated to elicit immunological responses in numerous cancer immunotherapy trials. However, long-lasting clinical effects are infrequent. We therefore sought to establish a protocol to generate DC with greater immunostimulatory capacity. Immature DC were generated from healthy donor monocytes by culturing in the presence of IL-4 and GM-CSF and were further differentiated into mature DC by the addition of cocktails containing different cytokines and toll-like receptor (TLR) agonists...
June 10, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28600293/bacterial-and-viral-products-affect-differential-pattern-recognition-receptor-activation-of-chicken-thrombocytes-evidenced-through-rna-sequencing
#17
Farzana Ferdous, Christopher Saski, William Bridges, Matthew Burns, Heather Dunn, Kathryn Elliott, Thomas R Scott
It is now well understood that thrombocytes (nucleated platelets) express TLRs and respond to both bacterial and viral products. Release of proinflammatory molecules can be expected following relatively short exposure times to LPS, lipoteichoic acid (LTA), thymidine homopolymer phosphorothioate oligonucleotide [Poly(dT)], and polyinosinic-polycytidylic acid [Poly(I:C)]. This study reports the varied expressions of genes encoded for components of the TLR, nucleotide binding oligomerization domain-like receptor, and retinoic acid-inducible gene RIG-like receptor signaling pathways in response to the TLR ligands listed above...
June 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28591694/the-nucleocapsid-proteins-of-mouse-hepatitis-virus-and-severe-acute-respiratory-syndrome-coronavirus-share-the-same-ifn-%C3%AE-antagonizing-mechanism-attenuation-of-pact-mediated-rig-i-mda5-activation
#18
Zhen Ding, Liurong Fang, Shuangling Yuan, Ling Zhao, Xunlei Wang, Siwen Long, Mohan Wang, Dang Wang, Mohamed Frahat Foda, Shaobo Xiao
Coronaviruses (CoVs) are a huge threat to both humans and animals and have evolved elaborate mechanisms to antagonize interferons (IFNs). Nucleocapsid (N) protein is the most abundant viral protein in CoV-infected cells, and has been identified as an innate immunity antagonist in several CoVs, including mouse hepatitis virus (MHV) and severe acute respiratory syndrome (SARS)-CoV. However, the underlying molecular mechanism(s) remain unclear. In this study, we found that MHV N protein inhibited Sendai virus and poly(I:C)-induced IFN-β production by targeting a molecule upstream of retinoic acid-induced gene I (RIG-I) and melanoma differentiation gene 5 (MDA5)...
May 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28588282/comparison-and-immunobiological-characterization-of-retinoic-acid-inducible-gene-i-like-receptor-expression-in-mesenchymal-stromal-cells
#19
Gordana Raicevic, Mehdi Najar, Hélène Busser, Emerence Crompot, Dominique Bron, Michel Toungouz, Laurence Lagneaux
Due to their immunomodulatory and regenerative properties, Mesenchymal stromal cells (MSC) have generated major interests in several clinical settings including transplantation and inflammatory diseases. MSC functions can be influenced by their tissue origin. Their microenvironment strongly affects their biology notably through TLR sensing. In this study, we show that MSC isolated from four different sources express another type of cytosolic pathogen recognition receptors known as retinoic acid inducible gene-I (RIG-I)-like receptors (RLR)...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588062/secondary-somatic-mutations-restoring-rad51c-and-rad51d-associated-with-acquired-resistance-to-the-parp-inhibitor-rucaparib-in-high-grade-ovarian-carcinoma
#20
Olga Kondrashova, Minh Nguyen, Kristy Shield-Artin, Anna V Tinker, Nelson N H Teng, Maria I Harrell, Michael J Kuiper, Gwo-Yaw Ho, Holly Barker, Maria Jasin, Rohit Prakash, Elizabeth M Kass, Meghan R Sullivan, Gregory J Brunette, Kara A Bernstein, Robert L Coleman, Anne Floquet, Michael Friedlander, Ganessan Kichenadasse, David M O'Malley, Amit M Oza, James X Sun, Liliane Robillard, Lara Maloney, David D L Bowtell, Heidi Giordano, Matthew J Wakefield, Scott H Kaufmann, Andrew D Simmons, Thomas C Harding, Mitch Raponi, Iain A McNeish, Elizabeth M Swisher, Kevin Lin, Clare L Scott
High-grade epithelial ovarian carcinomas (OC) containing mutated BRCA1 or BRCA2 (BRCA1/2) homologous recombination (HR) genes are sensitive to platinum-based chemotherapy and poly(ADP-ribose) polymerase inhibitors (PARPi), while restoration of HR function due to secondary mutations in BRCA1/2 has been recognized as an important resistance mechanism. We sequenced core HR pathway genes in 12 pairs of pre-treatment and post-progression tumor biopsy samples collected from patients in ARIEL2 Part 1, a phase 2 study of the PARPi rucaparib as treatment for platinum-sensitive, relapsed OC...
June 6, 2017: Cancer Discovery
keyword
keyword
106323
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"