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Angie D Ambers, Jennifer D Churchill, Jonathan L King, Monika Stoljarova, Harrell Gill-King, Mourad Assidi, Muhammad Abu-Elmagd, Abdelbaset Buhmeida, Bruce Budowle
BACKGROUND: Although the primary objective of forensic DNA analyses of unidentified human remains is positive identification, cases involving historical or archaeological skeletal remains often lack reference samples for comparison. Massively parallel sequencing (MPS) offers an opportunity to provide biometric data in such cases, and these cases provide valuable data on the feasibility of applying MPS for characterization of modern forensic casework samples. In this study, MPS was used to characterize 140-year-old human skeletal remains discovered at a historical site in Deadwood, South Dakota, United States...
October 17, 2016: BMC Genomics
Nathan J Hare, Ling Y Lee, Ian Loke, Warwick J Britton, Bernadette M Saunders, Morten Thaysen-Andersen
Tuberculosis (TB) remains a prevalent and lethal infectious disease. The glycobiology associated with Mycobacterium tuberculosis infection of front-line alveolar macrophages is still unresolved. Herein, we investigated the regulation of protein N-glycosylation in human macrophages and their secreted microparticles (MPs) used for intercellular communication upon M. tb infection. LC-MS/MS-based proteomics and glycomics were performed to monitor the regulation of glycosylation enzymes and receptors and the N-glycome in in vitro-differentiated macrophages and in isolated MPs upon M...
October 19, 2016: Journal of Proteome Research
Ling C Huang, Mercedes Salvador-Silva, Ronika S Leang
OBJECTIVES: To demonstrate correlations among in vitro assays used for assessing cytotoxicity of contact lens multipurpose solution (MPS) and propose the use of multiple assays as a part of preclinical evaluation for MPS biocompatibility assessment. METHODS: The effect of four different MPSs on cell cytotoxicity, metabolic activity, and membrane integrity was performed by evaluating toxicity, expression of tight junction protein zonula occludens-1, and transepithelial electrical resistance in human corneal epithelial cells and Chinese hamster fibroblasts cells...
October 3, 2016: Eye & Contact Lens
Fereshteh Esfandiari, Mohammad Kazemi Ashtiani, Mehdi Sharifi-Tabar, Maryam Saber, Hamed Daemi, Mohammad Hossein Ghanian, Abdolhossein Shahverdi, Hossein Baharvand
Producing meiosis-competent germ cells (GCs) from embryonic stem cells (ESCs) is essential for developing advanced therapies for infertility. Here, a novel approach is presented for generation of GCs from ESCs. In this regard, microparticles (MPs) have been developed from alginate sulfate loaded with bone morphogenetic protein 4 (BMP4). The results here show that BMP4 release from alginate sulfate MPs is significantly retarded by the sulfated groups compared to neat alginate. Then, BMP4-laden MPs are incorporated within the aggregates during differentiation of GCs from ESCs...
October 17, 2016: Macromolecular Bioscience
Sanaul Mustafa, V Kusum Devi, Roopa S Pai
Kanamycin sulphate (KS) is a Mycobacterium tuberculosis protein synthesis inhibitor. KS is polycationic, a property responsible for KS poor oral absorption half-life (2.5 h), and rapid renal clearance, which results in serious nephrotoxicity/ototoxicity. The current study aimed to develop KS-loaded PLGA-Vitamin-E-TPGS microparticles (MPs) and nanoparticles (NPs) to reduce the dosing frequency and dose related adverse effect. In vitro release was sustained up to 10 days for KS PLGA-TPGS MPs and 13 days for KS PLGA-TPGS NPs in PBS pH 7...
October 13, 2016: Journal of Microencapsulation
Carolina Gómez-Gaete, Macarena Retamal, Catalina Chávez, Paulina Bustos, Ricardo Godoy, Pablo Torres-Vergara
Rhein is an active metabolite of the drug diacerein, whose anti-inflammatory properties have been demonstrated in both in vitro and in vivo models. However, the low oral bioavailability of rhein has limited its utility as a potential treatment of osteoarthritis (OA), a chronic inflammatory disease. In order to overcome this limitation, the aim of this work was the development of a drug delivery system intended for intra-articular administration of rhein, based on polymeric biodegradable PLGA microparticles (MPs) loaded with the drug...
October 6, 2016: European Journal of Pharmaceutical Sciences
Francyne Kubaski, Heidi H Kecskemethy, H Theodore Harcke, Shunji Tomatsu
To date, the only published reports of bone mineral density (BMD) in MPS IV involve patients with MPS IVA; no reports exist describing BMD for MPS IVB. In this prospective study of BMD in three patients with MPS IVB, BMD was acquired by dual-energy X-ray absorptiometry (DXA) at whole body (WB), lumbar spine (LS), and lateral distal femur (LDF). Functional abilities, ambulatory status, medical history, and height z-score were evaluated. Three patients with MPS IVB (two females), aged 17.7, 31.4 and 31.7 years, were evaluated...
September 2016: Molecular Genetics and Metabolism Reports
Nicole M M Novroski, Jonathan L King, Jennifer D Churchill, Lay Hong Seah, Bruce Budowle
Massively parallel sequencing (MPS) can identify sequence variation within short tandem repeat (STR) alleles as well as their nominal allele lengths that traditionally have been obtained by capillary electrophoresis. Using the MiSeq FGx Forensic Genomics System (Illumina), STRait Razor, and in-house excel workbooks, genetic variation was characterized within STR repeat and flanking regions of 27 autosomal, 7 X chromosome and 24 Y-chromosome STR markers in 777 unrelated individuals from four population groups...
September 28, 2016: Forensic Science International. Genetics
Zhenbao Liu, Chunyan Wang, Yanfei Liu, Dongming Peng
Cefepime (CFP) is a frequently used antibiotic for prevention of post-surgery infection. Systemic delivery of CFP in a bulk dose usually shows effective therapeutic effects, while cytotoxicity can also be generated. To avoid the drawback of systemic delivery of antibiotic, local and controlled administration of drug is being employed to prolong therapeutic effects and reduce cytotoxicity by sustaining drug release and minimizing drug exposure. In this work, CFP loaded polymer O-carboxymethyl chitosan (OCMC) microspheres (CFP-OCMC-MPs) were fabricated and their antimicrobial activity against Staphylococcus aureus was evaluated...
October 4, 2016: Journal of Biomaterials Science. Polymer Edition
E Artoni, G L Sighinolfi, A M Gatti, M Sebastiani, M Colaci, D Giuggioli, C Ferri
BACKGROUND: Mixed cryoglobulinemia (MC) is a rare multisystem disease whose aetiopathogenesis is not completely understood. Hepatitis C virus (HCV) infection may have a causative role, and genetic and/or environmental factors may also contribute. AIMS: To investigate the presence and possible role of environmental agents in MC. METHODS: We recruited 30 HCV-infected MC patients with different clinical manifestations and a control group of 30 healthy, sex-/age-matched volunteers...
September 30, 2016: Occupational Medicine
Xiu-Ping He, Zi-Ru Lian, Li-Ju Tan, Jiang-Tao Wang
Highly selective and efficient magnetic molecularly imprinted polymers (MMIPs) were prepared using Fe3O4@SiO2 as a magnetic supporter, 3-methacryloxypropyltrimethoxy-silane (MPS) as a silane coupling agent, DIS as a template, methacrylic acid (MAA) as a functional monomer and ethyleneglycol dimethacrylate (EGDMA) as a cross-linker for the extraction of trace residuals of the synthetic estrogen dienestrol (DIS) in seawater, which is a concern worldwide for its endocrine disruption and carcinogenic danger to human health...
October 21, 2016: Journal of Chromatography. A
Sandra R Breyer, Nicole Muschol, Mona Schmidt, Martin Rupprecht, Kornelia Babin, Jochen Herrmann, Ralf Stücker
BACKGROUND: Hip dysplasia is common in mucopolysaccharidosis type-1H (MPS-1H) patients, but its morphology is not completely understood. No magnetic resonance imaging (MRI)-based studies have been reported in the literature. The purpose of this study was to improve knowledge of hip dysplasia pathology by describing the hip morphology of these patients in MRI scans, plain radiographs, and arthrograms. METHODS: We performed a retrospective chart review of 18 MPS-1H patients...
September 15, 2016: Journal of Pediatric Orthopedics
Lina Du, Junwei Jia, Pingju Ge, Yiguang Jin
Anti-HIV prodrugs are recently focused on due to their ability of self-assembly, macrophage targeting, and enhanced antiviral effects. Here, an amphiphilic prodrug of zidovudine, an anti-HIV nucleoside analogue, 5'-cholesteryl-ethyl-phosphoryl zidovudine (CEPZ) was synthesized. CEPZ showed some unique physicochemical properties. The solubility of CEPZ in the noncompetitive solvents chloroform and tetrahydrofuran (THF) was very high based on the hydrogen bonds between zidovudine groups, though CEPZ was sparing soluble in alcohols and almost insoluble in water...
September 8, 2016: Colloids and Surfaces. B, Biointerfaces
Timothy P Holsgrove, Nicolas V Jaumard, Nina Zhu, Nicholas S Stiansen, William C Welch, Beth A Winkelstein
Dynamic cervical spine loading can produce facet capsule injury. Despite a large proportion of neck pain being attributable to the C2/C3 facet capsule, potential mechanisms are not understood. This study replicated low-speed frontal and rear-end traffic collisions in Occiput-C3 human cadaveric cervical spine specimens, and used kinematic and full-field strain analyses to assess injury. Specimens were loaded quasistatically in flexion and extension before and after dynamic rotation of C3 at 100°/sec. Global kinematics in the sagittal plane were tracked at 1kHz and C2/C3 facet capsule full-field strains were measured...
September 16, 2016: Journal of Biomechanical Engineering
Taissia G Popova, Allison Teunis, Virginia Espina, Lance A Liotta, Serguei G Popov
In this study the hydrogel microparticles (MPs) were used to enhance migration of neutrophils in order to improve outcome of anthrax infection in a mouse model. Two MP formulations were tested. In the first one the polyacrylamide gel MPs were chemically coupled with Cibacron Blue (CB) affinity bait. In the second one the bait molecules within the MPs were additionally loaded with neutrophil-attracting chemokines (CKs), human CXCL8 and mouse CCL3. A non-covalent interaction of the bait with the CKs provided their gradual release after administration of the MPs to the host...
2016: PloS One
Shingo Hattori, Kengo Akagawa, Kazuaki Kudo, Kazuyuki Ishii
We demonstrated that the circular dichroism (CD) exciton chirality method, based on the supramolecular interactions of meso-tetra(4-sulfonatophenyl)porphyrin (MTPPS4, M = Zn or H2), was applicable for the determination of the absolute configuration between the side chains of two basic amino acid residues of stable monomeric β-hairpin peptides (tryptophan zipper: Trpzip). When MTPPS4 was added to an aqueous solution containing Trpzip, a bisignate CD signal was detected in the Soret band region in addition to a decrease in absorbance...
October 6, 2016: Journal of Physical Chemistry. B
L A Krueger, D C Beitz, S B Humphrey, J R Stabel
Peripheral blood mononuclear cells (PBMC) and mesenteric node lymphocytes (MNL) were obtained from 30 calves that were assigned randomly at birth to 1 of 6 treatment groups with 5 calves per treatment in a 14-d study: (1) colostrum-deprived (CD), no vitamins; (2) colostrum-replacer (CR), no vitamins; (3) CR, vitamin A; (4) CR, vitamin D3; (5) CR, vitamin E; (6) CR, vitamins A, D3, E. Calves were injected with appropriate vitamin supplements and fed pasteurized whole milk (CD calves) or fractionated colostrum replacer (CR calves) at birth...
September 7, 2016: Journal of Dairy Science
Liushiqi Borjigin, Tomoyuki Shimazu, Yuki Katayama, Kouichi Watanabe, Haruki Kitazawa, Sang-Gun Roh, Hisashi Aso, Kazuo Katoh, Masahiro Satoh, Yoshihito Suda, Akiko Sakuma, Mituru Nakajo, Keiichi Suzuki
To clarify the genetic influence of mycoplasmal pneumonia of swine (MPS) lesion-selected Landrace (La) on MPS resistance and immune characteristics in three-way crossbred pigs (LaWaDa), the LaWaDa pigs were compared with the non-selected crossbred (LbWbDb) and purebred (La) pigs. The MPS lesion score in the three lines was as follows: La line < LaWaDa line < LbWbDb line, with significant differences among the lines. The proportions of myeloid cells and T cells were lower and higher, respectively, in the LaWaDa pigs compared with those in the other two lines...
September 9, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
Christoph Kampmann, Tariq Abu-Tair, Seyfullah Gökce, Christina Lampe, Jörg Reinke, Eugen Mengel, Julia B Hennermann, Christiane M Wiethoff
BACKGROUND: Mucopolysaccharidosis (MPS) IVA is a rare lysosomal storage disorder with multiple skeletal and non-skeletal abnormalities requiring multiple surgical interventions. It is well known that patients with MPS IVA suffer from tachycardia, but cardiac and hemodynamic alterations have not been reported to date. We investigated the cardiovascular and hemodynamic alterations in patients with MPS IVA and developed a possible patho-mechanism for cardiovascular deterioration during anesthesia...
2016: PloS One
Manal M Gabriel, Cindy McAnally, John Bartell
OBJECTIVE: The aim of this study was to use antimicrobial efficacy endpoint methodology to determine compatibility of multipurpose disinfecting solutions (MPSs), lens cases, and hydrogel lenses for disinfection (AEEMC) against International Organization for Standardization (ISO)-specified microorganisms and clinical ocular isolates of Stenotrophomonas maltophilia. METHODS: Six MPSs (PQ/Aldox 1, 2, and 3; PQ/Alexidine; PQ/PHMB; and PHMB) were challenged against ISO-specified microorganisms and S...
September 2, 2016: Eye & Contact Lens
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