keyword
MENU ▼
Read by QxMD icon Read
search

MPS 3

keyword
https://www.readbyqxmd.com/read/28432840/detection-of-egfr-mutations-in-patients-with-non-small-cell-lung-cancer-by-high-resolution-melting-comparison-with-other-methods
#1
Carlos Martínez-Carretero, Fernando Iguaz Pascual, Antonio Rus, Ivan Bernardo
BACKGROUND: The discovery of mutations in the epidermal growth factor receptor gene (EGFR) related to the clinical response to tyrosine kinase inhibitors, has transformed the management of non-small cell lung cancer (NSCLC). Several methods have been developed for determination of mutations in EGFR, with different sensitivity and potential ability to detect a different number of mutations. METHODS: We developed a screening method by high resolution melting (HRM) to detect EGFR mutations, and compared the results of 123 fixed in formalin and paraffin embedded (FFPE) tumor tissue samples with the detection of mutations by allele-specific PCR...
April 22, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28429088/an-snp-panel-for-the-analysis-of-paternally-inherited-alleles-in-maternal-plasma-using-ion-torrent-pgm
#2
Donggui Yang, Hao Liang, Shaobin Lin, Qing Li, Xiaoyan Ma, Jun Gao, Hongyu Sun, Qingqing Chen, Jianzhu Wu, Xueling Ou
Researchers have sought to develop an effective protocol for paternity analysis using cell-free DNA (cfDNA) in maternal plasma. The use of massively parallel sequencing (MPS) technology for SNP testing is attractive because of its high-throughput capacity and resolution to single-base precision. In this study, we designed a customized SNP panel for cfDNA sequencing that includes 720 short amplicons (< 140 bp) targeting SNPs on the autosome and Y chromosome. The systemic performance was evaluated using the Ion Torrent PGM, indicating balanced coverage among most of the included loci, except for 78 poorly performing SNPs that were observed to have an inconsistent allele balance, lower coverage reads or high background signals...
April 20, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28428471/motion-frozen-imaging-by-gated-myocardial-perfusion-single-photon-emission-computed-tomography-using-multi-focus-fan-beam-collimator-in-thallium-201-study
#3
Kaito Takahashi, Takashi Takeuchi, Yoshiyuki Hosokai, Hayato Odagiri, Haruo Saito
PURPOSE: This study aimed to evaluate the statistical noise of motion-frozen (MF) image generated by gated myocardial perfusion single photon emission computed tomography (SPECT) imaging using IQ · SPECT and to determine the optimal acquisition and reconstruction parameters for MF image using IQ · SPECT. METHODS: A movement cardiac phantom and static cardiac phantom were used to acquire the MF images. The acquisition times used were different in 8 and 16 frames per R-R interval, and varying reconstruction parameters (subset and iteration) were used...
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/28426871/macular-pigment-density-and-quantitative-fundus-autofluorescence-in-young-healthy-subjects
#4
Chiara M Eandi, Marco Nassisi, Carlo Lavia, Camilla Alovisi, Ugo de Sanctis
Purpose: To measure macular pigment (MP) and find possible correlation between heterochromatic flicker photometry (HFP) and quantitative autofluorescence (qAF) in young healthy subjects. Methods: We enrolled 80 eyes of 40 young healthy subjects. Macular pigment optical density (MPOD) was automatically calculated with a macular pigment screener (MPS; MPODHFP). We calculated qAF comparing gray levels (GL) of qAF images with GL of internal reference of a confocal scanning laser ophthalmoscopy...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28424820/microparticles-during-long-term-follow-up-after-acute-myocardial-infarction-association-to-atherosclerotic-burden-and-risk-of-cardiovascular-events
#5
Christina Christersson, Åsa Thulin, Agneta Siegbahn
Microparticles (MPs) are formed from platelets (PMPs), endothelial cells (EMPs) and monocytes (MMPs), and in acute myocardial infarction (MI), there is an increase of MPs in the culprit artery. In this study MPs were evaluated in whole blood in 105 patients with MI at five time-points during a two-year follow-up (FU). Patients with non-ST-elevated MI had higher concentrations of CD41+MPs compared to ST-elevated MI patients (p=0.024). The concentrations of PMPs in whole blood increased during the time period (p<0...
April 20, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28409533/control-of-oxygen-tension-recapitulates-zone-specific-functions-in-human-liver-microphysiology-systems
#6
Felipe T Lee-Montiel, Subin M George, Albert H Gough, Anup D Sharma, Juanfang Wu, Richard DeBiasio, Lawrence A Vernetti, D Lansing Taylor
This article describes our next generation human Liver Acinus MicroPhysiology System (LAMPS). The key demonstration of this study was that Zone 1 and Zone 3 microenvironments can be established by controlling the oxygen tension in individual devices over the range of ca. 3 to 13%. The oxygen tension was computationally modeled using input on the microfluidic device dimensions, numbers of cells, oxygen consumption rates of hepatocytes, the diffusion coefficients of oxygen in different materials and the flow rate of media in the MicroPhysiology System (MPS)...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28409016/surface-complement-c3-fragments-and-cellular-binding-of-microparticles-in-patients-with-sle
#7
Line Kjær Winberg, Claus Henrik Nielsen, Søren Jacobsen
OBJECTIVES: To examine microparticles (MPs) from patients with SLE and healthy controls (HCs) by determining the cellular origin of the MPs, quantifying attached fragments of complement component 3 (C3) and assessing the ability of MPs to bind to circulating phagocytes and erythrocytes. These features may be relevant for clearance of MPs in SLE pathogenesis. METHODS: Attached C3 fragments (C3b, iC3b, C3d), membrane integrity and cell surface markers of MPs from 18 patients with SLE and 11 HCs were measured by adding specific antibodies, 7-aminoactinomycin D (7AAD) and annexin V...
2017: Lupus Science & Medicine
https://www.readbyqxmd.com/read/28405179/a-review-of-studies-of-the-proteomes-of-circulating-microparticles-key-roles-for-galectin-3-binding-protein-expressing-microparticles-in-vascular-diseases-and-systemic-lupus-erythematosus
#8
REVIEW
Christoffer T Nielsen, Ole Østergaard, Niclas S Rasmussen, Søren Jacobsen, Niels H H Heegaard
Subcellular microvesicles (MVs) have attracted increasing interest during the past decades. While initially considered as inert cellular debris, several important roles for MVs in physiological homeostasis, cancer, cardiovascular, and autoimmune diseases have been uncovered. Although still poorly understood, MVs are involved in trafficking of information from cell-to-cell, and are implicated in the regulation of immunity, thrombosis, and coagulation. Different subtypes of extracellular MVs exist. This review focuses on the cell membrane-derived shedded MVs (ranging in size from 200 to 1000 nm) typically termed microparticles (MPs)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28400539/mass-cytometry-deep-phenotyping-of-human-mononuclear-phagocytes-and-myeloid-derived-suppressor-cells-from-human-blood-and-bone-marrow
#9
Mikael Roussel, P Brent Ferrell, Allison R Greenplate, Faustine Lhomme, Simon Le Gallou, Kirsten E Diggins, Douglas B Johnson, Jonathan M Irish
The monocyte phagocyte system (MPS) includes numerous monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous, both phenotypically and functionally. In this study, we sought to characterize those diverse MPS phenotypes with mass cytometry (CyTOF). To identify a deep phenotype of monocytes, macrophages, and DCs, a panel was designed to measure 38 identity, activation, and polarization markers, including CD14, CD16, HLA-DR, CD163, CD206, CD33, CD36, CD32, CD64, CD13, CD11b, CD11c, CD86, and CD274...
April 11, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28391461/toxicological-assessment-of-tungsten-oxide-nanoparticles-in-rats-after-acute-oral-exposure
#10
Srinivas Chinde, Naresh Dumala, Mohammed Fazlur Rahman, Sarika Srinivas Kalyan Kamal, Srinivas Indu Kumari, Mohammed Mahboob, Paramjit Grover
Advances in and the rapid growth of the nanotechnology sector have escalated manufacture of nanoparticles (NPs), resulting in a significant increase in the probability of exposure of humans and wildlife to these materials. Many NPs have been found to exert genotoxicity. Therefore, genotoxicity studies are mandatory to assess the toxicity of NPs as a concern of succumbing to genetic diseases and cancers are universal. Tungsten oxide (WO3) NPs are being explored extensively in various fields. However, the toxicological data of WO3 NPs by oral route in mammals is limited...
April 8, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28387166/multi-scale-analysis-of-the-effect-of-nano-filler-particle-diameter-on-the-physical-properties-of-cad-cam-composite-resin-blocks
#11
Satoshi Yamaguchi, Sayuri Inoue, Takahiko Sakai, Tomohiro Abe, Haruaki Kitagawa, Satoshi Imazato
The objective of this study was to assess the effect of silica nano-filler particle diameters in a computer-aided design/manufacturing (CAD/CAM) composite resin (CR) block on physical properties at the multi-scale in silico. CAD/CAM CR blocks were modeled, consisting of silica nano-filler particles (20, 40, 60, 80, and 100 nm) and matrix (Bis-GMA/TEGDMA), with filler volume contents of 55.161%. Calculation of Young's moduli and Poisson's ratios for the block at macro-scale were analyzed by homogenization. Macro-scale CAD/CAM CR blocks (3 × 3 × 3 mm) were modeled and compressive strengths were defined when the fracture loads exceeded 6075 N...
May 2017: Computer Methods in Biomechanics and Biomedical Engineering
https://www.readbyqxmd.com/read/28386239/skeletal-muscle-myomir-are-differentially-expressed-by-endurance-exercise-mode-and-combined-essential-amino-acid-and-carbohydrate-supplementation
#12
Lee M Margolis, Holly L McClung, Nancy E Murphy, Christopher T Carrigan, Stefan M Pasiakos
Skeletal muscle microRNAs (myomiR) expression is modulated by exercise, however, the influence of endurance exercise mode, combined with essential amino acid and carbohydrate (EAA+CHO) supplementation are not well defined. This study determined the effects of weighted versus non-weighted endurance exercise, with or without EAA+CHO ingestion on myomiR expression and their association with muscle protein synthesis (MPS). Twenty five adults performed 90 min of metabolically-matched (2.2 VO2 L·m(-1)) load carriage (LC; performed on a treadmill wearing a vest equal to 30% of individual body mass) or cycle ergometry (CE) exercise, during which EAA+CHO (10 g EAA and 46 g CHO) or non-nutritive control (CON) drinks were consumed...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28383714/a-systems-approach-identifies-time-dependent-associations-of-multimorbidities-with-pancreatic-cancer-risk
#13
P Gomez-Rubio, V Rosato, M Marquez, C Bosetti, E Molina-Montes, M Rava, J Piñero, C W Michalski, A Farré, X Molero, M Löhr, L Ilzarbe, J Perea, W Greenhalf, M O'Rorke, A Tardón, T Gress, V M Barberà, T Crnogorac-Jurcevic, L Muñoz-Bellvís, E Domínguez-Muñoz, A Gutiérrez-Sacristán, J Balsells, E Costello, C Guillén-Ponce, J Huang, M Iglesias, J Kleeff, B Kong, J Mora, L Murray, D O'Driscoll, P Peláez, I Poves, R T Lawlor, A Carrato, M Hidalgo, A Scarpa, L Sharp, L I Furlong, F X Real, C La Vecchia, N Malats
Background: HASH(0x4147db0) Pancreatic ductal adenocarcinoma (PDAC) is usually diagnosed in late adulthood; therefore, many patients suffer or have suffered from other diseases. Identifying disease-patterns associated with PDAC risk may enable a better characterization of high-risk patients. Methods: HASH(0x4b0ade8) Multimorbidity patterns (MPs) were assessed from 17 self-reported conditions using hierarchical clustering, principal component, and factor analyses in 1705 PDAC cases and 1084 controls from a European population...
April 5, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28382446/quantification-and-characterization-of-microplastics-in-blue-mussels-mytilus-edulis-protocol-setup-and-preliminary-data-on-the-contamination-of-the-french-atlantic-coast
#14
Nam Ngoc Phuong, Aurore Zalouk-Vergnoux, Abderrahmane Kamari, Catherine Mouneyrac, Frederic Amiard, Laurence Poirier, Fabienne Lagarde
Microplastics (MPs) constitute a main environmental issue due to their threat to marine organisms and so far to humans. The lack of a fast standard protocol in MP isolation and identification from living organisms bring to challenge for the science. In this paper, an optimized protocol using potassium hydroxide 10% (KOH 10%; m/v) for digestion of mussel soft tissues (Mytilus edulis) and multi-steps of sedimentation has been developed. Efficiency higher than 99.9% of organic and mineral matter elimination was shown by application on mussels sampled on the French Atlantic coast...
April 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28380821/sensitivity-analysis-of-multilayer-microporous-polymer-structures-for-terahertz-volatile-gas-sensing
#15
Borwen You, Ja-Yu Lu
The sensitivity of multilayer microporous polymer structures (MPSs) for in situ and label-free organic vapor sensing is investigated in terahertz frequency. The porous structure provides a large hydrophilic surface area and numerous micropores to adsorb or fill polar vapors, thereby leading to greatly enhanced wave-analyte interaction with an apparent terahertz signal change. Different configurations of MPS with distinct geometric parameters are fabricated to study the structure-dependent sensitivity. The signal responses from the acetone-vapor-filled MPS, represented as effective absorption coefficient and refractive index variation, are proportional to the amounts of vaporized molecules...
March 6, 2017: Optics Express
https://www.readbyqxmd.com/read/28375632/tumor-acidity-nir-controlled-interaction-of-transformable-nanoparticle-with-biological-systems-for-cancer-therapy
#16
Dong-Dong Li, Yinchu Ma, Jinzhi Du, Wei Tao, Xiao-Jiao Du, Xian-Zhu Yang, Jun Wang
Precisely controlling the interaction of nanoparticles with biological systems (nano-bio interactions) from the injection site to biological targets shows great potential for biomedical applications. Inspired by the ability of nanoparticles to alter their physicochemical properties according to different stimuli, we explored the tumor acidity and near-infrared (NIR) light activated transformable nanoparticle (DA)TAT-NPIR&DOX. This nanoparticle consists of a tumor acidity-activated TAT (the TAT lysine residues' amines was modified with 2,3-dimethylmaleic anhydride (DA)), a flexible chain polyphosphoester core co-encapsulated a NIR dye IR-780 and DOX (doxorubicin)...
April 4, 2017: Nano Letters
https://www.readbyqxmd.com/read/28368445/vitamin-d-reduces-oxidative-stress-induced-procaspase-3-rock1-activation-and-mp-release-by-placental-trophoblasts
#17
Jie Xu, Xiuyue Jia, Yang Gu, David F Lewis, Xin Gu, Yuping Wang
Context: Increased microparticle (MP) shedding by placental trophoblasts contributes to maternal vascular inflammatory response and endothelial dysfunction in preeclampsia (PE). Vitamin D has beneficial effects in pregnancy, however its effect on trophoblast MP release has not been investigated. Objective: To investigate if vitamin D could protect trophoblasts from oxidative stress-induced MP release. Design: Placental trophoblasts were isolated from uncomplicated and preeclamptic placentas...
March 20, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28366851/laccase-methacrylyol-functionalized-magnetic-particles-highly-immobilized-reusable-and-efficacious-for-methyl-red-decolourization
#18
Jiahong Lin, Qiujie Lai, Yingju Liu, Shi Chen, Xueyi Le, Xiaohua Zhou
Magnetically retrievable formulations of laccase potentially promising for biomedical and environmental applications were constructed by immobilization of the enzyme on the surface of magnetic nanoparticles functionalized by siloxane layers with active amino and methacrylyol moieties. The functional magnetic particles were prepared using a hydrolytic polycondensation reaction of tetraethoxysilane with 3-methacryloxypropyltrimethoxysilane (MPS). The results indicated that the amounts of laccase loading on the methacrylyol functional magnetic particles were approximately 150mg/g under the optimum conditions...
March 31, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28360408/senescence-of-pancreas-in-middle-aged-rats-with-normal-vascular-function
#19
Mohamad Kassem, Zahid Rasul Niazi, Malak Abbas, Ali El Habhab, Guillaume Kreutter, Sonia Khemais-Benkhiat, Cyril Auger, Maria-Cristina Antal, Valérie B Schini-Kerth, Florence Toti, Laurence Kessler
BACKGROUND In organ transplantation, particularly pancreas transplantation, donor age is a determinant factor for graft survival. Physiological aging is crucial in the progressive deterioration of organs in adulthood. We compared the senescence and function features of pancreas and vascular tissues in young rats and middle-aged rats. MATERIAL AND METHODS Islet morphology and the area of cells secreting insulin or glucagon was investigated using immunohistology in young rats (12 weeks) and middle-aged rats (52 weeks) (n=8)...
March 31, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28359098/attenuation-of-resting-but-not-load-mediated-protein-synthesis-in-prostate-cancer-patients-on-androgen-deprivation
#20
Erik D Hanson, André R Nelson, Daniel W D West, John A Violet, Lannie O'Keefe, Stuart M Phillips, Alan Hayes
Context: Androgen deprivation therapy (ADT) is a common prostate cancer (PCa) treatment but results in muscular atrophy. Periodic increases in muscle protein synthesis (MPS) that occur after resistance exercise or protein intake may ameliorate this muscle loss, but the impact of these anabolic stimuli during ADT is unclear. Objective: To determine the acute MPS response to whey protein supplementation with and without resistance exercise during ADT. Design: Acute response in PCa patients vs age-matched controls (CON)...
March 1, 2017: Journal of Clinical Endocrinology and Metabolism
keyword
keyword
106262
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"