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https://www.readbyqxmd.com/read/28333935/dengue-seroprevalence-seroconversion-and-risk-factors-in-dhaka-bangladesh
#1
Parnali Dhar-Chowdhury, Kishor Kumar Paul, C Emdad Haque, Shakhawat Hossain, L Robbin Lindsay, Antonia Dibernardo, W Abdullah Brooks, Michael A Drebot
BACKGROUND: Dengue virus (DENV) activity has been reported in Dhaka, Bangladesh since the early 1960s with the greatest burden of dengue fever and dengue hemorrhagic fever cases observed in 2000. Since this time, the intensity of dengue activity has varied from year to year, and its determining factors remained relatively unknown. In light of such gaps in knowledge, the main objectives of this study were to determine the magnitude of seroprevalence and seroconversion among the surveyed population, and establish the individual/household level risk factors for the presence of DENV antibodies among all age groups of target populations in the city of Dhaka...
March 23, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28320174/etiological-agents-of-viral-meningitis-in-children-from-a-dengue-endemic-area-southeast-region-of-brazil
#2
Danilo B de Oliveira, Talitah M Candiani, Ana Paula M Franco-Luiz, Gabriel M F Almeida, Jônatas S Abrahão, Maria Rios, Roney S Coimbra, Erna G Kroon
Meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. CSF of 70 children with the presumptive diagnosis of viral meningitis was analyzed by real time PCR (qPCR)...
April 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28289727/seroprevalence-of-zika-virus-in-wild-african-green-monkeys-and-baboons
#3
Connor R Buechler, Adam L Bailey, Andrea M Weiler, Gabrielle L Barry, Meghan E Breitbach, Laurel M Stewart, Anna J Jasinska, Nelson B Freimer, Cristian Apetrei, Jane E Phillips-Conroy, Clifford J Jolly, Jeffrey Rogers, Thomas C Friedrich, David H O'Connor
Zika virus (ZIKV) has recently spread through the Americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. Although the natural reservoir of ZIKV remains poorly defined, the virus was first identified in a captive "sentinel" macaque monkey in Africa in 1947. However, the virus has not been reported in humans or nonhuman primates (NHPs) in Africa outside Gabon in over a decade. Here, we examine ZIKV infection in 239 wild baboons and African green monkeys from South Africa, the Gambia, Tanzania, and Zambia using combinations of unbiased deep sequencing, quantitative reverse transcription-PCR (qRT-PCR), and an antibody capture assay that we optimized using serum collected from captive macaque monkeys exposed to ZIKV, dengue virus, and yellow fever virus...
March 2017: MSphere
https://www.readbyqxmd.com/read/28284914/dengue-virus-serologic-prevalence-and-seroconversion-rates-in-children-and-adults-in-medellin-colombia-implications-for-vaccine-introduction
#4
Mabel Carabali, Jacqueline Kyungah Lim, Diana Carolina Velez, Andrea Trujillo, Jorge Egurrola, Kang Sung Lee, Jay S Kaufman, Luiz Jacinto DaSilva, Ivan Dario Velez, Jorge E Osorio
BACKGROUND: Dengue is an important public health problem worldwide. Recently a vaccine has been licensed in some countries of Latin America and Asia. Recommendations for dengue vaccine introduction include endemicity and high serologic prevalence of dengue in the territories considering its introduction. METHODS: We conducted a community-based survey to estimate dengue seroprevalence and age-specific seroconversion rates in a community in Medellin, Colombia, using dengue serologic test (IgG Indirect ELISA)...
March 8, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28254680/enhancing-the-sensitivity-of-dengue-virus-serotype-detection-by-rt-pcr-among-infected-children-in-india
#5
Syed Fazil Ahamed, Rosario Vivek, Shalini Kotabagi, Kaustuv Nayak, Anmol Chandele, Murali-Krishna Kaja, Anita Shet
Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay)...
February 28, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28187147/widespread-fear-of-dengue-transmission-but-poor-practices-of-dengue-prevention-a-study-in-the-slums-of-delhi-india
#6
Éric Daudé, Sumit Mazumdar, Vandana Solanki
BACKGROUND: This study has been conducted to throw light on the knowledge and practices related to dengue fever among the poor population living in Delhi's slums. MATERIALS: A household survey was conducted in 2013 among 3,350 households. The households were stratified by a number of variables related to socio-economic status and health events such as hospitalisation. The data collection was completed through face-to-face interviews conducted with the help of 25 field investigators...
2017: PloS One
https://www.readbyqxmd.com/read/28177091/prothrombin-time-prolongation-was-the-most-important-indicator-of-severe-bleeding-in-children-with-severe-dengue-viral-infection
#7
Kamolwish Laoprasopwattana, Jehrabeeyah Binsaai, Pornpimol Pruekprasert, Alan Geater
No abstract text is available yet for this article.
January 6, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28164489/multiplex-pcr-for-the-detection-of-10-viruses-causing-encephalitis-encephalopathy-and-its-application-to-clinical-samples-collected-from-japanese-children-with-suspected-viral
#8
Ngan T K Pham, Hiroshi Ushijima, Aksara Thongprachum, Quang D Trinh, Pattara Khamrin, Chikako Arakawa, Wakako Ishii, Shoko Okitsu, Shihoko Komine-Aizawa, Satoshi Hayakawa
BACKGROUND: Acute encephalitis is a serious neurological condition having a high mortality rate and affecting both children and adults. This study aimed to develop a multiplex PCR method for the simultaneous screening of clinical samples for the presence of the 10 viruses presently considered as the major viral causes of acute encephalitis/ encephalopathy in Asia. METHODS: Using previously published primers that have been widely used to screen for herpes virus-6, influenza A virus, human parechovirus, herpes simplex viruses 1 and 2, Japanese encephalitis virus, group A rotavirus, enterovirus, adenovirus, and dengue virus in clinical samples, a single-tube multiplex PCR assay was developed and was tested for its sensitivity and specificity...
January 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28159953/clinical-profile-of-dengue-infection-in-immune-compromised-children
#9
Amitabh Singh, Rachna Seth, Mohit Singla, S K Kabra, Rakesh Lodha
Review of records of children admitted with dengue infection was carried out to compare clinical and laboratory parameters, course of illness, and outcome between immune-compromised and immune competent patients. Statistically significant differences were found in days to platelet recovery (P=0.03), hepatic dysfunction (P= 0.04), and higher requirement of fluid (P= 0.01) in immunecompromised group.
February 2, 2017: Indian Pediatrics
https://www.readbyqxmd.com/read/28104185/causes-and-outcomes-of-sepsis-in-southeast-asia-a-multinational-multicentre-cross-sectional-study
#10
(no author information available yet)
BACKGROUND: Improved understanding of pathogens that cause sepsis would aid management and antimicrobial selection. In this study, we aimed to identify the causative pathogens of sepsis in southeast Asia. METHODS: In this multinational multicentre cross-sectional study of community-acquired sepsis and severe sepsis, we prospectively recruited children (age ≥30 days and <18 years) and adults (age ≥18 years) at 13 public hospitals in Indonesia (n=3), Thailand (n=4), and Vietnam (n=6)...
February 2017: Lancet Global Health
https://www.readbyqxmd.com/read/28103255/mitigating-diseases-transmitted-by-aedes-mosquitoes-a-cluster-randomised-trial-of-permethrin-impregnated-school-uniforms
#11
Pattamaporn Kittayapong, Phanthip Olanratmanee, Pongsri Maskhao, Peter Byass, James Logan, Yesim Tozan, Valérie Louis, Duane J Gubler, Annelies Wilder-Smith
BACKGROUND: Viral diseases transmitted via Aedes mosquitoes are on the rise, such as Zika, dengue, and chikungunya. Novel tools to mitigate Aedes mosquitoes-transmitted diseases are urgently needed. We tested whether commercially insecticide-impregnated school uniforms can reduce dengue incidence in school children. METHODS: We designed a cluster-randomised controlled trial in Thailand. The primary endpoint was laboratory-confirmed dengue infections. Secondary endpoints were school absenteeism; and impregnated uniforms' 1-hour knock-down and 24 hour mosquito mortality as measured by standardised WHOPES bioassay cone tests at baseline and after repeated washing...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28099233/primary-outcome-measures-in-pediatric-septic-shock-trials-a-systematic-review
#12
Kusum Menon, James Dayre McNally, Jerry J Zimmerman, Michael S D Agus, Katie O'Hearn, R Scott Watson, Hector R Wong, Mark Duffett, David Wypij, Karen Choong
OBJECTIVE: To evaluate all published pediatric randomized controlled trials of patients with septic shock from any cause to examine the outcome measures used, the strengths and limitations of these measurements and whether the trial outcomes met feasibility criteria. DATA SOURCES: We used a previously published database of pediatric critical care randomized controlled trials (PICUtrials.net) derived from searches of MEDLINE, EMBASE, LILACS, and CENTRAL. STUDY SELECTION: We included randomized controlled trials of interventions to children admitted to a PICU with septic or dengue hemorrhagic shock which were published in English...
March 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28069071/sporadic-cases-of-adult-measles-a-research-article
#13
Ranjan Premaratna, Nathasha Luke, Harsha Perera, Mahesh Gunathilake, Pubudu Amarasena, T G A Nilmini Chandrasena
BACKGROUND: Measles caused by a paramyxovirus, characterized by fever, malaise, cough, coryza conjunctivitis, a maculopapular rash is known to result in pneumonia, encephalitis and death. Fatal cases of measles in Sri Lanka are rare after implementation of the National Immunization Programme in 1984. Thereafter 0.1% case fatality rate was observed during October 1999-June 2000 which is a very low figure compared to other regional countries. Immunization guidelines were further revised in 2001, 2011 and in 2012 when additional immunization was recommended to age group 4-21 years; who are likely to have inadequate immunization, in order to achieve elimination of Measles by 2020...
January 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28040554/proteinuria-during-dengue-fever-in-children
#14
Anne-Claire Andries, Veasna Duong, Julien Cappelle, Sivuth Ong, Alexandra Kerleguer, Sowath Ly, Arnaud Tarantola, Paul F Horwood, Anavaj Sakuntabhai, Philippe Dussart, Philippe Buchy
OBJECTIVES: This study aimed to investigate proteinuria occurring during dengue disease in children and assess if measurement of this parameter can help physicians in the clinical management of patients. METHODS: Proteinuria was assessed by dipstick and quantified by urine protein:creatinine ratio (UPCR) in samples from patients hospitalized with a confirmed dengue infection and in healthy controls. RESULTS: The dipstick tested positive in 42...
February 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28034883/an-evidence-based-algorithm-for-early-prognosis-of-severe-dengue-in-the-outpatient-setting
#15
Minh Tuan Nguyen, Thi Nhan Ho, Van Vinh Chau Nguyen, Thanh Hung Nguyen, Manh Tuan Ha, Van Tram Ta, Le Da Ha Nguyen, Loi Phan, Khoi Quang Han, Thi Hue Kien Duong, Nguyen Bich Chau Tran, Wills Bridget, Marcel Wolbers, Cameron P Simmons
BACKGROUND: Early prediction of severe dengue could significantly assist patient triage and case management METHODS: We prospectively investigated 7563 children with ≤3 days of fever recruited in the outpatient departments of six hospitals in southern Vietnam between 2010 and 2013. The primary endpoint of interest was severe dengue (2009 WHO Guidelines) and pre-defined risk variables were collected at the time of enrolment to enable prognostic model development. RESULTS: The analysis population comprised 7544 patients, of whom 2060 (27...
December 28, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28030537/molecular-virologic-and-clinical-characteristics-of-a-chikungunya-fever-outbreak-in-la-romana-dominican-republic-2014
#16
Rose M Langsjoen, Rebecca J Rubinstein, Tiffany F Kautz, Albert J Auguste, Jesse H Erasmus, Liddy Kiaty-Figueroa, Renessa Gerhardt, David Lin, Kumar L Hari, Ravi Jain, Nicolas Ruiz, Antonio E Muruato, Jael Silfa, Franklin Bido, Matthew Dacso, Scott C Weaver
Since emerging in Saint Martin in 2013, chikungunya virus (CHIKV), an alphavirus transmitted by the Aedes aegypti mosquito, has infected approximately two million individuals in the Americas, with over 500,000 reported cases in the Dominican Republic (DR). CHIKV-infected patients typically present with a febrile syndrome including polyarthritis/polyarthralgia, and a macropapular rash, similar to those infected with dengue and Zika viruses, and malaria. Nevertheless, many Dominican cases are unconfirmed due to the unavailability and high cost of laboratory testing and the absence of specific treatment for CHIKV infection...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28002420/the-impact-of-the-newly-licensed-dengue-vaccine-in-endemic-countries
#17
Maíra Aguiar, Nico Stollenwerk, Scott B Halstead
BACKGROUND: With approximately 3 billion people at risk of acquiring the infection, dengue fever is now considered the most important mosquito-borne viral disease in the world, with 390 million dengue infections occurring every year, of which 96 million manifest symptoms with any level of disease severity. Treatment of uncomplicated dengue cases is only supportive and severe dengue cases require hospital intensive care. A vaccine now licensed in several countries and developed by Sanofi Pasteur (CYD-TDV, named Dengvaxia), was able to protect, in the first 25 months of the two Phase III, 66% of a subset of 9-16 year old participants...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27986543/magnitude-of-viremia-antigenemia-and-infection-of-circulating-monocytes-in-children-with-mild-and-severe-dengue
#18
Federico Perdomo-Celis, Doris M Salgado, Carlos F Narváez
Dengue is a major public health problem in tropical regions around the world. Viral and immune host factors determine the clinical courses of the infection. We analyzed the dynamics of viremia (by real-time polymerase chain reactions), antigenemia (through detection of the viral non-structural protein [NS]-1 by enzyme-linked immunosorbent assays) and the frequency of virus-infected peripheral blood mononuclear cells (PBMCs) (by multiparametric flow cytometry) in children with primary or secondary dengue virus (DENV) infection in mild to severe cases...
March 2017: Acta Tropica
https://www.readbyqxmd.com/read/27983492/meningitis-associated-with-simultaneous-infection-by-multiple-dengue-virus-serotypes-in-children-brazil
#19
Paula Eillanny Silva Marinho, Danilo Bretas de Oliveira, Talitah Michel Sanchez Candiani, Ana Paula Correia Crispim, Pedro Paulo Martins Alvarenga, Fabrizia Cristina Dos Santos Castro, Jonatas Santos Abrahão, Maria Rios, Roney Santos Coimbra, Erna Geessien Kroon
To determine the causes of viral meningitis, we analyzed 22 cerebrospinal fluid samples collected during the 2014-2015 dengue epidemics in Brazil. We identified 3 serotypes of dengue virus (DENV-1, -2, and -3), as well as co-infection with 2 or 3 serotypes. We also detected the Asian II genotype of DENV-2.
January 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27973402/the-influence-of-urbanization-modes-on-the-spatial-circulation-of-flaviviruses-within-ouagadougou-burkina-faso
#20
Florence Fournet, Stéphane Rican, Zoé Vaillant, Anna Roudot, Aude Meunier-Nikiema, Daouda Kassié, Roch K Dabiré, Gérard Salem
Dengue is an emerging infectious disease of global significance. Although this virus has been reported for a long time, its significance within the burden of diseases in West Africa is not obvious, especially in Burkina Faso. Our objective was to evaluate flavivirus presence in Ouagadougou (Burkina Faso) and the link between anti-flavivirus antibody seroprevalence and urbanization modes. A population-based cross-sectional survey was conducted and 3015 children were enrolled from Ouagadougou districts with different types and degrees of urbanization (with/without equipment and high/low building density)...
December 10, 2016: International Journal of Environmental Research and Public Health
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