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https://www.readbyqxmd.com/read/28814833/markers-of-oxidative-nitrosative-stress-and-inflammation-in-lung-tissue-of-rats-exposed-to-different-intravenous-iron-compounds
#1
Jorge E Toblli, Gabriel Cao, Jorge F Giani, Fernando P Dominici, Margarita Angerosa
Iron deficiency anemia is a frequent complication in clinical conditions such as chronic kidney disease, chronic heart failure, inflammatory bowel disease, cancer, and excessive blood loss. Given the ability of iron to catalyze redox reactions, iron therapy can be associated with oxidative stress. The lung is uniquely susceptible to oxidative stress, and little is known about the effects of intravenous iron treatment in this organ. This study characterized changes in markers of oxidative/nitrosative stress and inflammation in the lung of non-iron deficient, non-anemic rats treated with five weekly doses (40 mg iron per kg body weight) of low molecular weight iron dextran (LMWID), iron sucrose (IS), ferric carboxymaltose (FCM), ferumoxytol (FMX), iron isomaltoside 1000 (IIM), or saline (control)...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28810223/effects-of-an-allelochemical-in-phaeodactylum-tricornutum-filtrate-on-heterosigma-akashiwo-morphological-physiological-and-growth-effects
#2
Rui Wang, Qiaona Xue, Jiangtao Wang, Liju Tan, Qingchun Zhang, Yue Zhao, Donald M Anderson
The effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of morphological, physiological and biochemical characters. Growth experiments showed that H. akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM), the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity, chlorophyll-a content, membrane potential)...
August 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/28806754/short-term-prediction-of-solar-energy-in-saudi-arabia-using-automated-design-fuzzy-logic-systems
#3
Majid Almaraashi
Solar energy is considered as one of the main sources for renewable energy in the near future. However, solar energy and other renewable energy sources have a drawback related to the difficulty in predicting their availability in the near future. This problem affects optimal exploitation of solar energy, especially in connection with other resources. Therefore, reliable solar energy prediction models are essential to solar energy management and economics. This paper presents work aimed at designing reliable models to predict the global horizontal irradiance (GHI) for the next day in 8 stations in Saudi Arabia...
2017: PloS One
https://www.readbyqxmd.com/read/28800532/cell-cycle-arrest-mediated-by-cd-induced-dna-damage-in-arabidopsis-root-tips
#4
Weina Cui, Hetong Wang, Jie Song, Xia Cao, Hilary J Rogers, Dennis Francis, Chunyun Jia, Lizong Sun, Meifang Hou, Yuesuo Yang, Peidong Tai, Wan Liu
Accumulating evidence demonstrates that the aberrant expression of cell cycle regulation and DNA repair genes can result in abnormal cell proliferation and genomic instability in eukaryotic cells under different stresses. Herein, Arabidopsis thaliana (Arabidopsis) seedlings were grown hydroponically on 0.5 × MS media containing cadmium (Cd) at 0-2.5mgL(-1) for 5d of treatment. Real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that expression of DNA damage repair and cell cycle regulation genes, including BRCA1, MRE11, WEE1, CDKA;1 and PCNA1, showed an inverted U-shaped dose-response...
August 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28799818/the-expression-and-clinical-signification-of-pd-1-in-lymph-nodes-of-patients-with-non-small-cell-lung-cancer
#5
Haitao Ma, GuoCai Mao, GuangBo Zhang, HaiTao Huang
To screen anti-programmed cell death protein 1 (PD-1) antibody treatment of the dominant population, it is necessary to understand the expression of PD-1 in tumor metastasis microenvironment. The aim of the present study was to detect the expression of PD-1 in lymph nodes of 51 patients with non-small cell lung cancer (NSCLC) by using flow cytometry (FCM). The results showed that the PD-1 expression on CD3(+) T cells was significantly increased in NSCLC metastatic lymph nodes (50.08 ± 8.03%) compared with nonmetastatic lymph nodes (36...
August 11, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28796073/effect-of-polysaccharide-of-dendrobium-candidum-on-proliferation-and-apoptosis-of-human-corneal-epithelial-cells-in-high-glucose
#6
Qiangxiang Li, Jing Chen, Yajia Li, Ting Chen, Jing Zou, Hua Wang
BACKGROUND: The aim of the study was to observe the effect of polysaccharide of dendrobium candidum (PDC) and high glucose on proliferation, apoptosis of human corneal epithelial cells (HCEC). METHODS: The MTT method was used to screen and take the optimal high-glucose concentration, treatment time, and PDC concentration using HCEC and divide it into 4 groups: control group (C), high glucose group (HG), PDC group, and HG + PDC group. We observed and compared the effect of the 4 groups on HCEC proliferation by MTT, apoptosis by Annexin V-FITC/PI double fluorescent staining and flow cytometry (FCM), and expression of bax mRNA and bcl-2 mRNA by RT-qPCR...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28792696/the-role-of-cytokines-in-the-peripheral-blood-of-major-depressive-patients
#7
Yili Chen, Juan Ouyang, Sujie Liu, Shihong Zhang, Peisong Chen, Tang Jiang
BACKGROUND: Recent technological advances offer an opportunity to further elucidate the complex cytokine network in Major Depressive Disorder (MDD). The objective of this study was to investigate the role of pro-inflammatory and anti-inflammatory cytokines in the mechanism of depressive disorders. Given the activating role of cytokines on the hypothalamic-pituitary-adrenal (HPA) axis, and the relevance of its regulation in MDD, we also analyzed the relationships between several cytokines and cortisol levels...
July 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28776671/%C3%AE-asarone-inhibited-cell-growth-and-promoted-autophagy-via-p53-bcl-2-bclin-1-and-p53-ampk-mtor-pathways-in-human-glioma-u251-cells
#8
Nanbu Wang, Qinxin Zhang, Laiyu Luo, Baile Ning, Yongqi Fang
Glioma is the most common type of primary brain tumor and has an undesirable prognosis. Autophagy plays an important role in cancer therapy, but it's effect is still not definite. P53 is an important tumor suppressor gene and protein that is closely to autophagy. Our aim was to study the effect of β-asarone on inhibiting cell proliferation in human glioma U251 cells and to detect the effect of the inhibition on autophagy through the P53 signal pathway. For cell growth, the cells were divided into four groups: the model, β-asarone, temozolomide (TMZ) and co-administration groups...
August 4, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28772041/budget-impact-of-intravenous-iron-therapy-with-ferric-carboxymaltose-in-patients-with-chronic-heart-failure-and-iron-deficiency-in-germany
#9
Ulrike Theidel, Saku Väätäinen, Janne Martikainen, Erkki Soini, Thomas Hardt, Wolfram Doehner
AIMS: Treatment of iron deficiency (ID) in patients with heart failure (HF) with intravenous iron substitution [ferric carboxymaltose (FCM)] has previously shown significant improvements in exercise capacity, New York Heart Association (NYHA) functional class, quality of life, and reduction of hospitalization. The aim of this study was to estimate the budget impact of FCM treatment for patients with HF and ID. METHODS AND RESULTS: Individual patient data from four double-blind randomized controlled trials were pooled for this analysis...
August 2017: ESC Heart Failure
https://www.readbyqxmd.com/read/28770951/microrna-181c-functions-as-a-protective-factor-in-a-1-methyl-4-phenylpyridinium-iodide-induced-cellular-parkinson-s-disease-model-via-bcl2l11
#10
M Wei, L-J Cao, J-L Zheng, L-J Xue, B Chen, F Xiao, C-S Xu
OBJECTIVE: Parkinson is one of the most common neurodegenerative diseases. At present, many studies have pointed out that miRNAs play a very important role in Parkinson's development and process. MiR-181c has been shown to have a significant low expression in blood samples and brain tissues of Parkinson's patients. MATERIALS AND METHODS: We used 1-Methyl-4-Phenylpyridinium Iodide (MPP(+)) as a tool for constructing the Parkinson's cell model, using mir181c mimics to construct an experimental model of acquisition...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28765940/il%C3%A2-33-restricts-invasion-and-adhesion-of-trophoblast-cell-line-jeg3-by-downregulation-of-integrin-%C3%AE-4%C3%AE-1-and-cd62l
#11
Xiao-Hui Wang, Wei Liu, Deng-Xuan Fan, Wen-Ting Hu, Ming-Qing Li, Xiao-Yong Zhu, Li-Ping Jin
Interleukin-33 (IL-33) promotes migration of cancer cells through downregulating the expression of E-cadherin. Previous studies have demonstrated that IL‑33 stimulates the proliferation of trophoblasts. However, the effect of IL‑33 on the adhesion and invasion of trophoblasts has not been investigated in detail. In the present study, the expression of IL‑33 and its receptor, IL‑1 receptor‑like 1 (ST2), was examined in villi from women during early pregnancy using immunohistochemistry. ST2 expression on human trophoblast and choriocarcinoma cell lines JAR, BeWo, JEG3 and HTR8 was confirmed by flow cytometry (FCM) assay...
July 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28764810/cd106-is-a-novel-mediator-of-bone-marrow-mesenchymal-stem-cells-via-nf-%C3%AE%C2%BAb-in-the-bone-marrow-failure-of-acquired-aplastic-anemia
#12
Shihong Lu, Meili Ge, Yizhou Zheng, Jianping Li, Xiaoming Feng, Sizhou Feng, Jinbo Huang, Ying Feng, Donglin Yang, Jun Shi, Fang Chen, Zhongchao Han
BACKGROUND: Acquired aplastic anemia (AA) is characterized by deficiency or dysfunction of the bone marrow (BM) microenvironment. However, little is known about the impairment of BM-derived mesenchymal stem cells (MSCs) in AA patients. METHODS: We used Illumina HiSeqTM 2000 sequencing, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry (FCM), and Western blotting to test the expression of CD106 gene (vascular cell adhesion molecule 1 (VCAM1)) and CD106 protein of BM-MSCs...
August 1, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28764262/relationship-between-automated-coronary-calcium-volumes-and-a-set-of-manual-coronary-lumen-volume-vessel-volume-and-atheroma-volume-in-japanese-diabetic-cohort
#13
Sumit K Banchhor, Narendra D Londhe, Luca Saba, Petia Radeva, John R Laird, Jasjit S Suri
INTRODUCTION: A high degree of correlation exists between Coronary Artery Diseases (CAD) and calcification of the vessel wall. For Percutaneous Coronary Interventional (PCI) planning, it is essential to have an exact understanding of the extent to which calcium volume is correlated to the lumen, vessel, and atheroma volume regions in the coronary artery, which is unclear in recent studies. AIM: Four automated Coronary Calcium Volume (aCCV) measurement methods {threshold, Fuzzy c-Means (FCM), K-means, and Hidden Markov Random Field (HMRF)} and its correlation with three manual (experts) coronary parameters namely: Coronary Vessel Volume (mCVV), Coronary Lumen Volume (mCLV), and Coronary Atheroma Volume (mCAV), was determined in a Japanese diabetic cohort...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28758033/efficacy-and-tolerability-of-intravenous-ferric-carboxymaltose-in-patients-with-iron-deficiency-at-a-hospital-outpatient-clinic-a-retrospective-cohort-study-of-real-world-clinical-practice
#14
António Robalo Nunes, Ana Palricas Costa, Sara Lemos Rocha, Ana Garcia de Oliveira
Ferric carboxymaltose (FCM) is an intravenous iron formulation to correct iron deficiency. Although its use has been extensively studied in clinical trials, real-world evidence regarding FCM treatment is scarce. Our aim was to evaluate the efficacy and tolerability of FCM treatment in patients with iron deficiency, with or without anemia, at a hospital outpatient clinic. Data was collected retrospectively from medical records. During this 2-year study, 459 patients were included. Mean age was 58.6 ± 17.5 years and most patients received cumulative FCM doses of 501-1000 mg (63...
2017: Anemia
https://www.readbyqxmd.com/read/28753934/rapid-estimation-of-astaxanthin-and-the-carotenoid-to-chlorophyll-ratio-in-the-green-microalga-chromochloris-zofingiensis-using-flow-cytometry
#15
Junhui Chen, Dong Wei, Georg Pohnert
The green microalga Chromochloris zofingiensis can accumulate significant amounts of valuable carotenoids, mainly natural astaxanthin, a product with applications in functional food, cosmetics, nutraceuticals, and with potential therapeutic value in cardiovascular and neurological diseases. To optimize the production of astaxanthin, it is essential to monitor the content of astaxanthin in algal cells during cultivation. The widely used HPLC (high-performance liquid chromatography) method for quantitative astaxanthin determination is time-consuming and laborious...
July 19, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28749127/fuzzy-c-means-clustering-based-segmentation-and-cnn-classification-for-accurate-segmentation-of-lung-nodules
#16
Jalal Deen K, Ganesan R, Merline A
Objective: Accurate segmentation of abnormal and healthy lungs is very crucial for a steadfast computer-aided disease diagnostics. Methods: For this purpose a stack of chest CT scans are processed. In this paper, novel methods are proposed for segmentation of the multimodal grayscale lung CT scan. In the conventional methods using Markov–Gibbs Random Field (MGRF) model the required regions of interest (ROI) are identified. Result: The results of proposed FCM and CNN based process are compared with the results obtained from the conventional method using MGRF model...
July 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28745810/flow-cytometry-vs-optical-microscopy-in-the-evaluation-of-the-genotoxic-potential-of-xenobiotic-compounds
#17
Monia Lenzi, Veronica Cocchi, Patrizia Hrelia
BACKGROUND: It is now recognized that mutational events play a key role in the development of pathological processes like cancer, cardiovascular and neurodegenerative disease. Therefore, it is crucial to have Genetics Toxicology tests that allow rapid and accurate identification of the mutagenic potential of a xenobiotic. Currently the most widely used technique is the "In vitro mammalian cell micronucleus test" performed by optical microscopy, but some problems have been highlighted, including the number of cells analyzed, the high subjectivity of the reading at the microscope and the long analysis times...
July 26, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28739395/sterigmatocystin-induced-apoptosis-in-human-pulmonary-cells-in-vitro
#18
Jinfeng Cui, Juan Wang, Shujuan Huang, Xiujuan Jiang, Yuehong Li, Wenxin Wu, Xianghong Zhang
Sterigmatocystin (ST) is generally recognized as a potential carcinogen, mutagen and teratogen. Studies showed that ST could induce adenocarcinoma of lung in mice in vivo and DNA damage, cell cycle arrest in a human immortalized bronchial epithelial cell line (BEAS-2B cells) and a human lung cancer cell line (A549 cells) in vitro. Besides, ST could induce G2 arrest (cell cycle arrest in G2 phase) in several other cells. Cell cycle arrest may be one of the common toxic effects of ST. As cells may undergo apoptosis or death due to cell cycle arrest, we wondered whether apoptosis is another common effect of ST in different cells in vitro...
July 21, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28730944/integrating-bioassays-and-analytical-chemistry-as-an-improved-approach-to-support-safety-assessment-of-food-contact-materials
#19
Julien Veyrand, Maricel Marin-Kuan, Claudine Bezencon, Nancy Frank, Violaine Guérin, Sander Koster, Hélia Latado, Julie Mollergues, Amaury Patin, Dominique Piguet, Patrick Serrant, Jesus Varela, Benoît Schilter
Food contact materials (FCM) contain chemicals which can migrate into food and result in human exposure. Although it is mandatory to ensure that migration does not endanger human health, there is still no consensus on how to pragmatically assess the safety of FCM since traditional approaches would require extensive toxicological and analytical testing which are expensive and time consuming. Recently, the combination of bioassays, analytical chemistry and risk assessment has been promoted as a new paradigm to identify toxicologically relevant molecules and address safety issues...
July 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28727075/amino-acid-digestibility-by-weanling-pigs-of-processed-ingredients-originating-from-soybeans-00-rapeseeds-or-a-fermented-mixture-of-plant-ingredients
#20
D M D L Navarro, Y Liu, T S Bruun, H H Stein
An experiment was conducted to determine the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of CP and AA in 4 sources of processed soybean products, in conventional dehulled soybean meal (SBM-CV), in conventional 00-rapeseed expellers (RSE), and in a fermented coproduct mixture (FCM) that contained rapeseed meal, wheat, soy molasses, and potato peel fed to weanling pigs. The 4 processed soybean products included 2 sources of enzyme-treated soybean meal (ESBM-1 and ESBM-2), extruded soybean meal, and soy protein concentrate (SPC)...
June 2017: Journal of Animal Science
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