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https://www.readbyqxmd.com/read/28332730/multicenter-validation-of-a-simplified-method-for-paroxysmal-nocturnal-hemoglobinuria-screening
#1
Arianna Gatti, Luigi Del Vecchio, Massimo Geuna, Matteo G Della Porta, Bruno Brando
According to the most credited and validated international guidelines, testing peripheral blood for the diagnosis of PNH is best accomplished with four color, two tubes or five to six color, single tube flow cytometric (FCM) procedures, with the demonstration of the deficiency of at least two GPI-linked surface proteins in two different cell subsets to corroborate the diagnosis (1-7). Each one of these state-of-the-art techniques ensure a high degree of sensitivity (i.e. down to 0.01% PNH granulocyte or red cell clones) with a high level of standardization and good reproducibility...
March 23, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28325441/a-review-of-fuzzy-cognitive-maps-in-medicine-taxonomy-methods-and-applications
#2
REVIEW
Abdollah Amirkhani, Elpiniki I Papageorgiou, Akram Mohseni, Mohammad R Mosavi
BACKGROUND AND OBJECTIVE: A high percentage of medical errors, committed because of physician's lack of experience, huge volume of data to be analyzed, and inaccessibility to medical records of previous patients, can be reduced using computer-aided techniques. Therefore, designing more efficient medical decision-support systems (MDSSs) to assist physicians in decision-making is crucially important. Through combining the properties of fuzzy logic and neural networks, fuzzy cognitive maps (FCMs) are among the latest, most efficient, and strongest artificial intelligence techniques for modeling complex systems...
April 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28324281/detection-of-minimal-residual-disease-in-childhood-b-acute-lymphoblastic-leukemia-by-4-color-flowcytometry
#3
Ahmad Baraka, Laila M Sherief, Naglaa M Kamal, Shereen El Shorbagy
Monitoring of minimal residual disease (MRD) is currently considered the most powerful predictor of outcome in acute lymphoblastic leukemia (ALL). Achievement of a negative MRD state assessed by multicolor flowcytometry (MFC) is an important predictor of disease-free survival (DFS) and overall survival (OS) in ALL patients. We sought to determine whether panels of antibodies combination are more suitable for detection of MRD in Childhood ALL. Eighty-four (84) patients with ALL (B-lineage subtype) were enrolled in this study...
March 21, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28306547/synthetic-ct-for-mri-based-liver-stereotactic-body-radiotherapy-treatment-planning
#4
Jeremy S Bredfeldt, Lianli Liu, Mary Feng, Yue Cao, James M Balter
A technique for generating MRI-derived synthetic CT volumes (MRCTs) is demonstrated in support of adaptive liver stereotactic body radiation therapy (SBRT). Under IRB approval, 16 subjects with hepatocellular carcinoma were scanned using a single MR pulse sequence (T1 Dixon). Air-containing voxels were identified by intensity thresholding on T1-weighted, water and fat images. The envelope of the anterior vertebral bodies was segmented from the fat image and fuzzy-C-means (FCM) was used to classify each non-air voxel as mid-density, lower-density, bone, or marrow in the abdomen, with only bone and marrow classified within the vertebral body envelope...
March 17, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28303727/enhancement-of-nk-cells-proliferation-and-function-by-shikonin
#5
Yan Li, Hailin Lu, Yongchun Gu, Zhiqiang Ning, Tinghua Cao, Chao Chen, Chengru Hu, Min Tao
CONTEXT: Shikonin is a kind of naphthoquinone compound found mainly in Lithospermum erythrorhizon Sieb,et Zucc. Previous studies have shown that Shikonin has anti-tumor, anti-inflammatory and extensive pharmacological effects. According to new studies, Shikonin could also modulate the immune system function, but the effect to NK (nature killer) cells is yet unknown. OBJECTIVE: To investigate the effect and mechanism of Shikonin on NK cells proliferation and cytotoxicity to colon cancer cell line (Caco-2)...
March 17, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28301803/injectable-and-thermally-contractible-hydroxypropyl-methyl-cellulose-fe3o4-for-magnetic-hyperthermia-ablation-of-tumors
#6
Fengjuan Wang, Yang Yang, Yi Ling, Jianxin Liu, Xiaojun Cai, Xiaohan Zhou, Xiuzhen Tang, Bing Liang, Yini Chen, Hangrong Chen, Dengming Chen, Chunhong Li, Zhigang Wang, Bing Hu, Yuanyi Zheng
The development of efficient strategies for the magnetic hyperthermia ablation of tumors remains challenging. To overcome the significant safety limitations, we developed a thermally contractible, injectable and biodegradable material for the minimally invasive and highly efficient magnetic hyperthermia ablation of tumors. This material was composed of hydroxypropyl methyl cellulose (HPMC), polyvinyl alcohol (PVA) and Fe3O4. The thermal contractibility of HPMC/Fe3O4 was designed to avoid damaging the surrounding normal tissue upon heating, which was confirmed by visual inspection, ultrasound imaging and computed tomography (CT)...
March 8, 2017: Biomaterials
https://www.readbyqxmd.com/read/28293405/diagnostic-utility-of-flow-cytometry-in-myelodysplastic-syndromes
#7
REVIEW
Matteo G Della Porta, Cristina Picone
The pathological hallmark of myelodysplastic syndromes (MDS) is marrow dysplasia, which represents the basis of the WHO classification of these disorders. This classification provides clinicians with a useful tool for defining the different subtypes of MDS and individual prognosis. The WHO proposal has raised some concern regarding minimal diagnostic criteria particularly in patients with normal karyotype without robust morphological markers of dysplasia (such as ring sideroblasts or excess of blasts). Therefore, there is clearly need to refine the accuracy to detect marrow dysplasia...
2017: Mediterranean Journal of Hematology and Infectious Diseases
https://www.readbyqxmd.com/read/28286781/generation-of-monoclonal-antibodies-against-immunoglobulin-proteins-of-the-domestic-ferret-mustela-putorius-furo
#8
Greg A Kirchenbaum, Ted M Ross
The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28283666/clinical-parameters-and-adrenocortical-activity-to-assess-stress-responses-of-alpacas-using-different-methods-of-restraint-either-alone-or-with-shearing
#9
T Wittek, T Salaberger, R Palme, S Becker, F Hajek, B Lambacher, S Waiblinger
Shearing of alpacas is stressful and is undertaken by restraint in the standing position, cast on the floor or on a tilt table. The objectives of the study were to evaluate and compare the stress responses between different methods. The study consisted of two parts. In part one, 15 animals were restrained applying all three methods but without shearing. In part two, 45 animals in three groups of 15 were shorn using one of the three procedures. Body temperature, heart rate, respiratory rate, salivary cortisol and faecal cortisol metabolites (FCM) were measured...
March 10, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28282379/tensor-based-multichannel-reconstruction-for-breast-tumours-identification-from-dce-mris
#10
X-X Yin, S Hadjiloucas, J-H Chen, Y Zhang, J-L Wu, M-Y Su
A new methodology based on tensor algebra that uses a higher order singular value decomposition to perform three-dimensional voxel reconstruction from a series of temporal images obtained using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is proposed. Principal component analysis (PCA) is used to robustly extract the spatial and temporal image features and simultaneously de-noise the datasets. Tumour segmentation on enhanced scaled (ES) images performed using a fuzzy C-means (FCM) cluster algorithm is compared with that achieved using the proposed tensorial framework...
2017: PloS One
https://www.readbyqxmd.com/read/28276325/ferric-carboxymaltose-induced-hypophosphataemia-after-kidney-transplantation
#11
V Sari, R Atiqi, E J Hoorn, A C Heijboer, T van Gelder, D A Hesselink
BACKGROUND: Ferric carboxymaltose (FCM) can induce hypophosphataemia in the general population and patients with chronic kidney disease (CKD). Less is known about the effect of FCM in the kidney transplant population. It has been suggested that fibroblast growth factor 23 (FGF-23)-mediated renal phosphate wasting may be the most likely cause of this phenomenon. In the current study, the effects of FCM on phosphate metabolism were studied in a cohort of kidney transplant recipients. METHODS: Two index patients receiving FCM are described...
March 2017: Netherlands Journal of Medicine
https://www.readbyqxmd.com/read/28269516/randomness-in-resting-state-functional-connectivity-matrices
#12
Victor M Vergara, Vince Calhoun
Separate brain regions exhibit synchronous intrinsic activity used to assess connectivity patterns known to appear among brain areas. Connectivity is evaluated from functional magnetic resonance imaging (fMRI) measuring the blood oxygen level dependent signal (BOLD) signal. Extensive research has revealed a distinctive pattern of connectivity among brain areas that can be visualized through a functional connectivity matrix (FCM) matrix. As in any measurement, BOLD signals are subject to contamination from noise and nuisances unrelated to brain's intrinsic activity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28269166/impaired-episodic-memory-network-in-subjects-at-high-risk-for-alzheimer-s-disease
#13
Yafeng Zhan, Jianhua Ma, Kaibin Xu, Yanhui Ding, Yue Cui, Zhengyi Yang, Yong Liu
Episodic memory dysfunction is one of the hallmark symptoms of Alzheimer's disease (AD) and mild cognitive impairment (MCI). This cognitive impairment may be related to abnormal brain structure and activity. Functional connectivity mapping (FCM) analysis provides a powerful tool for exploring the topology of human brain function using magnetic resonance imaging (MRI). Thus, it would be advantageous to investigate the changes in functional connectivity within the episodic memory network in a longitudinal MCI dataset, as it may be helpful in identifying a potential marker of disease progress...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28260650/characterizations-of-cd4-1-cd4-2-and-cd8%C3%AE-t-cell-subpopulations-in-peripheral-blood-leucocytes-spleen-and-head-kidney-of-japanese-flounder-paralichthys-olivaceus
#14
Jing Xing, Junjie Ma, Xiaoqian Tang, Xiuzhen Sheng, Wenbin Zhan
In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4(+) and CD8(+) lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4-1, CD4-2 and CD8β recombinant proteins and their antibodies (Abs) were produced, then the cross-reactivity of the Abs to CD4-1, CD4-2 and CD8β was detected by Western blotting, respectively, and the reactions of Abs to PBL were analyzed by immunofluorescence staining (IFS) and flow cytometry (FCM)...
March 2, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28251801/origin-of-enriched-regulatory-t-cells-in-patients-receiving-combined-kidney-bone-marrow-transplantation-to-induce-transplantation-tolerance
#15
Ben Sprangers, Susan DeWolf, Thomas M Savage, Tatsuaki Morokata, Aleksandar Obradovic, Samuel A LoCascio, Brittany Shonts, Julien Zuber, Sai Ping Lau, Ravi Shah, Heather Morris, Valeria Steshenko, Emmanuel Zorn, Frederic I Preffer, Sven Olek, David M Dombkowski, Laurence A Turka, Robert Colvin, Robert Winchester, Tatsuo Kawai, Megan Sykes
We examined tolerance mechanisms in patients receiving HLA-mismatched combined kidney and bone marrow transplantation (CKBMT) that led to transient chimerism under a previously-published non-myeloablative conditioning regimen (Immune Tolerance Network study ITN036). Polychromatic flow cytometry (FCM) and high throughput sequencing of TCRβ hypervariable regions of DNA from peripheral blood T regulatory cells (Tregs) and CD4 non-Tregs revealed marked early enrichment of regulatory T cells (CD3(+) CD4(+) CD25(high) CD127(low) Foxp3(+) ) in blood that resulted from peripheral proliferation (Ki67(+) ), possibly new thymic emigration (CD31(+) ) and, in one tolerant subject, conversion from non-Tregs...
March 1, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28245394/-levels-and-significance-of-serum-interleukin-35-and-the-new-regulatory-t-cells-itr35-cells-in-myelodysplastic-syndrome-patients
#16
Wen-Qian Xu, Yuan-Mao Huang, Hui-Fang Xiao
OBJECTIVE: To investigate the significance of serum interleukin-35 level and the new regulatory T cells -iTR35 cells in patients with myelodysplastic syndrome (MDS). METHODS: Twenty three cases of newly diagnosed MDS were enrolled in this study from January 2014 to January 2016 in Department of Hematology of The First Hospital of Quanzhou in Fujian Province. According to MDS International Prognostic Scoring System (IPSS), the 23 patients were divided into 4 groups: high-risk (n=4), intermediate risk-2 (n=10), intermediate risk-1 (n=5) and low-risk group(n=4)...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28245381/-effect-of-arsenic-trioxide-on-k562-cell-proliferation-and-its-mechanism
#17
Yuan Wang, Jie Yang, Jie Li, Rui-Cang Wang, Jun Yuan, Yan Li, Su-Yun Wang, Chao Wang, Hong-Ling Hao
OBJECTIVE: To investigate the molecular mechanism of arsenic trioxide(ATO) inhibiting K562 cell proliferation, and explore the new targets for treating chronic myeloid leukemia(CML). METHODS: human CML cell line K562 cells were cultured in vitro, and were treated with different concentrations of ATO; MTT was used to detect the cell proliferation; flow cytometry(FCM) was used to determine cell apoptosis, cell cycle and the expression of CD44; Transcriptional levels of β-catenin and cyclin D1 were assayed by RT-PCR...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28245371/-expression-of-cd146-in-adult-and-children-s-acute-b-cell-lymphoblastic-leukemia-and-its-significance
#18
Xiao-Qing Xie, Wei-Min Wang, Si-Lin Gan, Sheng-Mei Chen, Qiu-Tang Zhang, Xin-Sheng Xie, Yan-Fang Liu, Yuan-Dong Cheng, Yu-Feng Liu, Xue-Ju Xu, Hui Sun
OBJECTIVE: To explore the differences of CD146 expression in adult and children's acute B cell lymphoblastic leukemia(B-ALL), and its relation with clinical features, molecular biological and cytogenctic claracteristics. METHODS: The expression of CD146 in bone marrow samples from adult and children's B-ALL patients were detected by flow cytometry (FCM) and the relation of CD146 abnormal high expression with the patients' clinical features, molecular biological and cytogenetical characteristics, as well as other antigens were analyzed...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28243324/11r-p53-and-gm-csf-expressing-oncolytic-adenovirus-target-cancer-stem-cells-with-enhanced-synergistic-activity
#19
Sai-Qun Lv, Zhen-Long Ye, Pin-Yi Liu, Yao Huang, Lin-Fang Li, Hui Liu, Hai-Li Zhu, Hua-Jun Jin, Qi-Jun Qian
Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcinoma stem cells Hep3B-C and teratoma stem cells ECCG5. Firstly, the augmenting effect of 11R in our construct was tested and confirmed by examining the expression of EGFP with Fluorescence and FCM assays after transfecting Hep3B-C and ECCG5 cells with OVA SG7605-EGFP and SG7605-11R-EGFP...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28239823/inhibitory-effect-and-mechanism-of-metformin-on-human-ovarian-cancer-cells-skov-3-and-a2780
#20
J Huo, X-H Bian, Y Huang, Z-C Miao, L-H Song
OBJECTIVE: Ovarian cancer is the most common malignant tumor in female reproductive system. Metformin is an orally taken hypoglycemic agent, which is extensively applied in the clinic. Clinical trials find that there may be a certain degree of action of the metformin in inhibiting malignant tumors. This paper aims to investigate the inhibitory effect and mechanism of metformin on human ovarian cancer cells. MATERIALS AND METHODS: Through in vitro cell experiment, the influences of metformin on the proliferation, colony formation and apoptosis of ovarian carcinoma cells were studied...
February 2017: European Review for Medical and Pharmacological Sciences
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