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Jun-Wei Shen, Ming Shan, Yuan-Yuan Peng, Ting-Jun Fan
PURPOSE: To investigate the cytotoxic effect of latanoprost on corneal stroma and its underlying cellular and molecular mechanisms using non-transfected human corneal stromal (HCS) cells as an in vitro model. METHODS: After HCS cells were treated with latanoprost at concentrations varying from 50 mg/l (clinical therapeutic dosage) to 0.78125 mg/l, and cell morphology, cell viability, and cell cycle were detected by light microscopy, methyl thiazolyl tetrazolium assay, and flow cytometry (FCM) with propidium iodide (PI) staining, respectively...
October 17, 2016: Current Eye Research
Jitka Sosnovcová, Marián Rucki, Hana Bendová
AIM: The presented work characterized components of food contact materials (FCM) with potential to bind to estrogen receptor (ER) and cause adverse effects in the human organism. METHODS: The QSAR Toolbox, software application designed to identify and fill toxicological data gaps for chemical hazard assessment, was used. Estrogen receptors are much less of a lock-and-key interaction than highly specific ones. The ER is nonspecific enough to permit binding with a diverse array of chemical structures...
September 2016: Central European Journal of Public Health
Luca Fortunato, Sanghyun Jeong, Yiran Wang, Ali R Behzad, TorOve Leiknes
Fouling in membrane bioreactors (MBR) is acknowledged to be complex and unclear. An integrated characterization methodology was employed in this study to understand the fouling on a gravity-driven submerged MBR (GD-SMBR). It involved the use of different analytical tools, including optical coherence tomography (OCT), liquid chromatography with organic carbon detection (LC-OCD), total organic carbon (TOC), flow cytometer (FCM), adenosine triphosphate analysis (ATP) and scanning electron microscopy (SEM). The three-dimensional (3D) biomass morphology was acquired in a real-time through non-destructive and in situ OCT scanning of 75% of the total membrane surface directly in the tank...
October 1, 2016: Bioresource Technology
Xiao-Su Zhao, Ya-Zhen Qin, Yan-Rong Liu, Ying-Jun Chang, Lan-Ping Xu, Xiao-Hui Zhang, Xiao-Jun Huang
The minimal residual disease (MRD) before and after a haploidentical hematopoietic stem cell transplantation (HSCT) of 86 patients with acute myeloid leukemia (AML) in complete remission (CR) was measured using flow cytometry (FCM) and Wilms tumor 1 (WT1). In all, 18 patients met the criteria of pre-MRD + before HSCT. The FCM + (p = .028) and the combinative criteria for positive MRD (MRDco+) (p = .022) post-transplantation were significantly correlated to relapse in univariate analysis. A multivariate analysis showed that only MRDco + post-transplantation was an independent risk factor of leukemia relapse (p = ...
October 12, 2016: Leukemia & Lymphoma
Jiang-Rui Guo, Wei Li, Yong Wu, Lin-Qing Wu, Xin Li, Ya-Fei Guo, Xiao-Hui Zheng, Xiao-Lan Lian, Hui-Fang Huang, Yuan-Zhong Chen
This study aims to investigate effects of HGF expression on biological behaviors of Kasumi-1 and HL60. Expression of HGF and c-Met gene were detected using qRT-PCR. Short hairpin RNA (shRNA) was used to reduce HGF expression. Silencing effect of shRNA was verified by qRT-PCR and western blot. Cell reproductive capacity, cell clonality and cell cycle (apoptosis) were detected by CCK-8, clone formation, flow cytometry (FCM), respectively. Cell adhesion, cell invasion ability and cell proliferation were also examined...
2016: American Journal of Translational Research
Rupak Banik, Subhadip Roy, Ľubor Dlháň, Ján Titiš, Roman Boča, Alexander M Kirillov, Adam D Martin, Antonio Bauza, Antonio Frontera, Antonio Rodríguez-Diéguez, Juan M Salas, Subrata Das
A new iron(iii) complex [Fe(H2Vi)3]·py·4H2O (1) {H3Vi = violuric acid, py = pyridine} was self-assembled and characterized. In the crystal structure, adjacent [Fe(H2Vi)3] blocks are H-bonded into a complex 3D network, which was topologically classified as a pcu [alpha-Po primitive cubic] network. Importance of π-stacking interactions was also highlighted and analyzed computationally. Magnetic studies confirmed antiferromagnetic exchange coupling, while ZFCM/FCM experiments indicated an ordering temperature Tc = 50 K...
October 18, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Stephanie Karmann, Stéphanie Follonier, Monica Bassas-Galia, Sven Panke, Manfred Zinn
Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Its bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of carbon substrates and other nutrients. These dynamics require a fast and quantitative method to determine the optimal harvest-time of PHA containing cells or to adjust carbon supply. In principle, flow cytometry (FCM) is an ideal tool that suits these requirements and, in addition, provides data on the PHA content of different cell populations...
October 6, 2016: Journal of Microbiological Methods
Henry Hui, Kathy A Fuller, Wendy N Erber, Matthew D Linden
Platelets are subcellular blood elements with a well-established role in haemostasis. Upon activation platelets undergo granule exocytosis, resulting in α-granule P-Selectin being expressed on the cell membrane. This allows binding of activated platelets to P-Selectin glycoprotein ligand 1 (PSGL-1) expressing leukocytes, forming leukocyte-platelet aggregates (LPAs). Whole blood flow cytometry (FCM) has demonstrated that elevated circulating LPAs (especially monocyte LPAs) are linked to atherothrombosis in high risk patients, and that activated platelet binding influences monocytes towards a pro-adhesive and pro-atherogenic phenotype...
October 5, 2016: Methods: a Companion to Methods in Enzymology
Jian Kang, Jixin Zhang, Yongqian Bai
An evaluation of the oil-spill emergency response capability (OS-ERC) currently in place in modern marine management is required to prevent pollution and loss accidents. The objective of this paper is to develop a novel OS-ERC evaluation model, the importance of which stems from the current lack of integrated approaches for interpreting, ranking and assessing OS-ERC performance factors. In the first part of this paper, the factors influencing OS-ERC are analyzed and classified to generate a global evaluation index system...
October 4, 2016: Marine Pollution Bulletin
Nishant Jain, Vinod Kumar
In this paper we have proposed an iterative Fuzzy C-Mean (IFCM) method which divides the pixels present in the image into a set of clusters. This set of clusters is then used to segment a focal liver lesion from a liver ultrasound image. Advantage of IFCM methods is that n-clusters FCM method may lead to non-uniform distribution of centroids, whereas in IFCM method centroids will always be uniformly distributed. Proposed method is compared with the edge based Active contour Chan-Vese (CV) method, and MAP-MRF method by implementing the methods on MATLAB...
November 2016: Journal of Medical Systems
Chen Zhu, Ni-Rong Bao, Shuo Chen, Jian-Ning Zhao
The aim of the study was to investigate the mechanisms of human β-defensin 3 (HBD-3) regulation of the immune response and the lipopolysaccharide/Toll-like receptor-4 (LPS/TLR4)-mediated signaling pathway. A TLR4 extracellular gene fragment was cloned into the pET32a plasmid to determine its expression in Escherichia coli (E. coli) and purification. A dialysis labeling method was used to stain HBD-3 with fluorescein isothiocyanate (FITC). FITC-HBD-3 was used to induce the differentiation of human peripheral blood mononuclear cells (MNC) into immature dendritic cells (imDC) in vitro...
October 2016: Experimental and Therapeutic Medicine
Xiaochen Guo, Hongxue Cao, Yunxin Wang, Yang Liu, Yanmin Chen, Nan Wang, Shan Jiang, Shengqi Zhang, Qiang Wu, Tianhe Li, Yingjie Zhang, Bing Zhou, Jiechao Yin, Deshan Li, Guiping Ren
We have previously reported an antigen and antibody co-expression (AAC) technology to demonstrate the interaction between a known antigen and antibody. To validate the co-expression system for screening antibody libraries, a single chain fragment variable(scFv)antibody library was constructed from chickens immunized with the VP2 protein of infectious bursal disease virus (IBDV). The genes of both VP2 and scFv antibodies were inserted into the pBFD-Ab-Ag vector. The co-expression library was subjected to three rounds of screening by flow cytometry (FCM) using a polyclonal antibody against VP2 through a bacteria display system...
November 1, 2016: Veterinary Immunology and Immunopathology
Shu-Jun Li, Yan-Xia Wu, Hua-Lin Chen, Mei-Lian Liu, Ai-Bing Wu, Zhi-Xiong Yang
OBJECTIVE: To investigate the correlation of CD4(+)CD29(+) regulatory T cells (Treg) with tumor recurrence and survival time in patients with non-small cell lung cancer (NSCLC). METHODS: Fifty-nine patients with NSCLC treated with radical surgery were followed up for 5 years. Blood Treg cells were examined during the follow-up using flow cytometry (FCM). The sensitivity and specificity of Treg cells to predict recurrence of NSCLC were analyzed using receiver-operating characteristic (ROC) curve and compared with those of carcinoembryonic antigen (CEA) and cytokeratin21-1 (Cyfra21-1)...
August 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Li-Ming Xu, Jing-Zhuang Zhao, Miao Liu, Yong-Sheng Cao, Jia-Sheng Yin, Hong-Bai Liu, Tongyan Lu
Infectious pancreatic necrosis is a significant disease of farmed salmonids in China. In this study, a single chain variable fragment (scFv) antibody library derived from rainbow trout (Oncorhynchus mykiss) and viral protein VP2 of a Chinese infectious pancreatic necrosis virus (IPNV) isolate ChRtm213 were co-expressed by a bacterial display technology. The library was subjected to three rounds of screening by flow cytometry (FCM) to select IPNV specific antibodies. Six antibody clones with different mean fluorescence intensities (MFI) were obtained by picking colonies at random...
November 2016: Journal of Virological Methods
Huimin Xu, Jianhua Jin, Lu Chen, Chunxiao Li, Qinggang Xu, Juanjuan Shi, Buchang Zhao, Yongzhong Hou, Hong Wang
Naoxintong (NXT) is an empirical formula based on the principle of traditional Chinese medicine, which has been approved by China Food and Drug Administration (CFDA) and is widely used for treatment of patients with cerebrovascular and cardiovascular diseases in China. The aim of this study is to investigate the protective mechanism of NXT on H9c2 cells (cardiogenic cell line) in response to H2O2. MTT, Western blot, and flow cytometry (FCM) methods were used to identify the protective effect of NXT extract on H2O2-induced H9c2 cells...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Zhaoliang Su, Yongjian Zhang, Ping Ni, Jia Wang
Objective To investigate the effects of recombinant high mobility group box l (rHMGB1) on the differentiation of myeloid-derived suppressor cells (MDSCs) in vitro. Methods The cells were harvested from the bone marrow of BALB/c mice and cultured with rHMGB1, granulocyte-macrophage colony-stimulating factor (GM-CSF) combined with IL-6 (GM-CSF-IL-6), GM-CSF combined with IL-6 and rHMGB1 (GM-CSF-IL-6-rHMGB1), separately in vitro. The percentages of CD11b(+)Gr1(+)MDSCs, CD11c(+) cells and F4/80(+) macrophages were measured by flow cycometry (FCM) after treatment for 48 hours...
October 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Chencheng Fang, Jiming Zhang, Nan Lu, Peng Li, Yu Fan, Yuexi Kang, Chun Yang, Yonglin He
Objective To observe the effect of zinc-dependent metalloprotease 1 (Zmp1) of Bacillus Calmette Guerin vaccine (BCG) on the proliferation and phagosome-lysosome fusion of RAW264.7 mouse macrophage. Methods Zmp1 was expressed in E.coli BL21 (DE3) and purified by metal chelate magnetic beads. RAW264.7 cells were incubated with the purified Zmp1. Cell proliferation was detected at 0, 24, 48, 72 and 96 hours by cell counting kit-8 (CCK-8) assay and cell cycle distribution was detected by flow cytometry (FCM). The formation of phagosome was induced after RAW264...
October 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Marylin Rangassamy, Sara Khaleghparast Athari, Raquel Monclús, Marie-Christophe Boissier, Natacha Bessis, Heiko G Rödel
The way how individuals respond to chronic challenges can vary tremendously, and such differences are closely linked to personality. The few available studies on individual differences in stress-related immunosuppression in non-human mammals have been mainly carried out with laboratory strains. We conducted a study in male mound-building mice (Mus spicilegus) of wild origin. We distinguished between high (HAN) and low anxious/neophobic (LAN) personality types, quantified by subjects' consistent and associated behavioral responses in repeated elevated plus maze and novel object tests...
September 22, 2016: Physiology & Behavior
Magdalena A Olszewska, Aleksandra M Kocot, Anna Nynca, Łucja Łaniewska-Trokenheim
pH stress is recognized as an important feature for Lactobacillus in relation to lifestyle and commercial utility. Hence, this study aims to investigate the cell function of Lactobacilli cells subjected to pHs between 7.0 and 2.0. For this purpose, the Lactobacilli isolates of vegetable origin were first hybridized with fluorescent oligonucleotide rRNA probes for detecting Lactobacillus species. Then, cells were exposed to pH stress and labelled with fluorescent probes, carboxyfluorescein diacetate (CFDA) and propidium iodine (PI), which provided the insight into esterase activity and membrane integrity of cells...
November 2016: Microbiological Research
M Sánchez-Castañon, B G Castro, M Toca, C Santacruz, M Arias-Loste, P Iruzubieta, J Crespo, Marcos López-Hoyos
AIM: The enumeration of intraepithelial lymphocytes subsets (total, γδ, and CD3(-) IELs) by flow cytometry (FCM), named as IEL lymphogram, constitutes a useful tool for celiac disease (CD) diagnosis. The aim of this study was to quantify IELs by FCM and their diagnostic value to differentiate active, silent and potential CD. METHODS: Prospective study of 60 active and 20 silent CD patients, and 161 controls in which duodenal biopsy and IEL quantification by FCM was performed...
December 2016: Auto- Immunity Highlights
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