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Razvan Ghinea, Luis Javier Herrera, María M Pérez, Ana M Ionescu, Rade D Paravina
OBJECTIVE: To design colorimetric and spectral models of gingival shade guides that adequately represent the color of human gingiva. MATERIALS AND METHODS: A previously compiled database on the spectral reflectance of healthy keratinized gingiva was used for optimization. Coverage Error (CE) and Maximal Error (ME) were optimized using CIELAB and CIEDE2000 color difference formulas. A two-phase process included an FCM algorithm and a nonlinear optimization. A t test was used to compare the performance of the different numbers of clusters/tabs in gingival shade guide models (α = ...
March 2018: Journal of Esthetic and Restorative Dentistry
Chao Li, Jingfang Zheng, Si Chen, Bin Huang, Guosheng Li, Zichao Feng, Jiwei Wang, Shujun Xu
Glioblastoma pathogenesis is related to multiple processes that affected by dozens of regulatory factors, but the potential underlying factors regulating glioblastoma progression remains unclear. The goal of this research was to determine how the ribonucleotide reductase M2 subunit (RRM2) influenced proliferation, invasion, migration, and apoptosis of human glioblastoma cells. The level of proliferation of human glioblastoma cells was measured through CCK8, colony formation assay and immunofluorescence stains...
April 18, 2018: Journal of Cellular Physiology
Yan Zhou, Li-Lan Li, Zhou-Lin Zhong, Xue-Jun Liu, Jin-Lian Liu, Wei-Dong Shen, Guo-Guang Wu
OBJECTIVE: To analyse the cases of platelet transfusion refractoriness after received HLA-matched unrelated donor hematopoietic stem cell transplantation, to analyze and identify the phenotype and genotype of CD36 in both the patient and stem cell donor, as well as the characteristic of antibody induced platelet transfusion refractoriness, and to analyse the efficacy of matched CD36-deficiency platelets transfusions. METHODS: The CD36 expression on platelet and monocyte was analyzed by flow cytometry (FCM) in both patient and donor...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jie Liu, Ben-Nian Huo, Ting Zhang, Meng-Nan Liu, Xue Wang, Tao Feng
OBJECTIVE: To investigate the effect of embryonic stem cells on the proliferation and apoptosis in human acute myeloid leukemia cell line KG-1a and to explore its potential mechanism. METHODS: The direct co-culture system between human embryonic stem cells H9 and human acute myeloid leukemia cell line KG-1a was established, and CCK8 assay was used to detect the proliferation of KG-1a cells. The changes of cell cycle and apoptosis were detected by flow cytometry (FCM)...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Bin-Bin Yu, Guo-Xiang Lin, Ling Li, Song Qu, Zhong-Guo Liang, Kai-Hua Chen, Lei Zhou, Qi-Teng Lu, Yong-Chu Sun, Xiao-Dong Zhu
BACKGROUND The purpose of this study was to determine whether cofilin-2 could serve as a protein marker for predicting radiotherapy response and as a potential therapeutic target in nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS Cofilin-2 protein levels in serum and tissue samples from patients with NPC were assessed by sandwich ELISA and IHC. In vitro, cofilin-2 levels in CNE-2R cells were significantly higher than those of CNE-2 cells. Meanwhile, CNE-2R cells were silenced for cofilin-2 to obtain a stable cofilin-2-RNAi-LV3 cell line...
April 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Hui Shi, Yinting Mao, Qianqian Ju, Yingcheng Wu, Wen Bai, Peiwen Wang, Yudong Zhang, Maorong Jiang
C-terminal binding protein‑2 (CtBP2) is a transcriptional co-repressor that is associated with tumorigenesis and tumor progression. It has been reported to predict a poor prognosis in several human cancers, including esophageal squamous cell carcinoma (ESCC). The present study aimed to investigate the involvement of CtBP2 in the cisplatin (DDP) resistance of the ECA109 ESCC cell line and its effect on the expression of apoptosis-associated proteins. Constructed recombinant lentiviruses were used for the knockdown or overexpression of CtBP2 in ECA109 cells, and the expression of CtBP2 was measured using reverse transcription-quantitative polymerase chain reaction and western blotting following transfection...
April 12, 2018: International Journal of Oncology
Dominique Fauteux, Gilles Gauthier, Dominique Berteaux, Rupert Palme, Rudy Boonstra
Non-consumptive effects of predation have rarely been assessed in wildlife populations even though their impact could be as important as lethal effects. Reproduction of individuals is one of the most important demographic parameters that could be affected by predator-induced stress, which in turn can have important consequences on population dynamics. We studied non-consumptive effects of predation on the reproductive activity (i.e., mating and fertilization) of a cyclic population of brown lemmings exposed to intense summer predation in the Canadian High Arctic...
April 12, 2018: Oecologia
Xuelei Yin, Shuaishuai Feng, Yingying Chi, Jinhu Liu, Kaoxiang Sun, Chuanyou Guo, Zimei Wu
An estrogen (ES)-functionalized cationic liposomal system was developed and exploited for targeted delivery to osteosarcoma. Natural biocompatible chotooligosaccharides (COS, MW2-5 KDa) were covalently tethered to the liposomal surface through a disulfate bond (-SS-) to confer reduction-responsive COS detachment, whereas estrogen was grafted via polyethylene glycol (PEG 2 K) chain to achieve estrogen receptor-targeting. The liposomal carriers were prepared by the ethanol injection method and fluorescent anticancer drug doxorubicin (DOX) was loaded with ammonium sulfate gradient...
November 2018: Drug Delivery
Ankur Ahuja, Seema Tyagi, Tulika Seth, Hara Prasad Pati, Gps Gahlot, Preeti Tripathi, Venkatesan Somasundaram, Renu Saxena
Acute Myeloid Leukemia (AML) as per World Health Organization (WHO 2008) classification is on the basis of the antigenic characterization, enzymes restriction in the neoplastic myeloid cells and the specific translocations/mutations. AML can be assessed and differentiated by flowcytometry (FCM)/immunohistochemistry (IHC)/cytochemistry techniques. Myeloperoxidase (MPO) is an unequivocal marker to differentiate AML from the acute lymphoblastic leukemia. Despite FCM popularity, it has its limitations, in form of 'dry-tap', cost, and inability of being performed by retrospective analysis...
April 2018: Indian Journal of Hematology & Blood Transfusion
Lei Zheng, Zhi Li, Wei Ling, Deming Zhu, Zhiwen Feng, Lianbao Kong
BACKGROUND/AIMS: The present study aimed to evaluate the effects as well as the underlying mechanisms of bone marrow-derived dendritic cells (BMDCs) and exosomes produced by BMDCs (DEXs) on hepatic ischemia-reperfusion (I/R) injury (IRI). METHODS: Primary hepatocytes were isolated and used to mimic the liver IR microenvironment. BMDCs were induced and characterized both biochemically with a flow cytometer (FCM) and biophysically with a microscope. Then, we exposed BMDCs to the supernatants from primary hepatocytes and evaluated the maturation of BMDCs by FCM...
March 29, 2018: Cellular Physiology and Biochemistry
Wenhai Lin, Lei Yin, Tingting Sun, Tingting Wang, Zhigang Xie, Jingkai Gu, Xiabin Jing
Fundamental studies on the cellular uptake and drug release of PEGylated nanomedicines are beneficial to understand their fate in vivo and construct ideal nanoparticle formulations. In this work, the detailed metabolic process of PEGylated doxorubicin (Dox) nanomedicines were investigated via confocal laser scanning microscopy (CLSM), flow cytometry (FCM), cytotoxicity test, fluorescence imaging in vivo (FLIV) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Among them, only LC-MS/MS could determine accurately the content of PEGylated Dox and Dox in vitro and in vivo...
April 2, 2018: Biomacromolecules
Gema Miñana, Ingrid Cardells, Patricia Palau, Pau Llàcer, Lorenzo Fácila, Luis Almenar, Maria Pilar López-Lereu, Jose V Monmeneu, Martina Amiguet, Jessika González, Alicia Serrano, Vicente Montagud, Raquel López-Vilella, Ernesto Valero, Sergio García-Blas, Vicent Bodí, Rafael de la Espriella-Juan, Juan Sanchis, Francisco J Chorro, Antoni Bayés-Genís, Julio Núñez
BRIEF TRIAL SUMMARY BACKGROUND: Treatment with intravenous ferric carboxymaltose (FCM) showed to improve symptoms, functional capacity, and quality of life in patients with heart failure (HF) and iron deficiency (ID). However, the underlying mechanisms for these beneficial effects remain undetermined. The aim of this study is to quantify cardiac magnetic resonance (CMR) changes in myocardial iron content after administration of intravenous FCM in patients with HF and ID and contrast them with parameters of HF severity...
April 1, 2018: Clinical Cardiology
Li Xu, Jing Du, Caifeng Wan, Yu Zhang, Shaowei Xie, Hongli Li, Hong Yang, Fenghua Li
Background: The development of nanoscale molecularly targeted ultrasound contrast agents (UCAs) with high affinity and specificity is critical for ultrasound molecular imaging in the early detection of breast cancer. Purpose: To prospectively evaluate ultrasound molecular imaging with dual-targeted gold nanoshelled poly(lactide-co-glycolic acid) nanocapsules carrying vascular endothelial growth factor receptor type 2 (VEGFR2) and p53 antibodies (DNCs) in MCF-7 orthotopic mice model...
2018: International Journal of Nanomedicine
Victor M Vergara, Qingbao Yu, Vince D Calhoun
BACKGROUND: Functional magnetic resonance imaging (fMRI) allows for the measurement of functional connectivity of the brain. In this context, graph theory has revealed distinctive non-random connectivity patterns. However, the application of graph theory to fMRI often utilizes non-linear transformations (absolute value) to extract edge representations. NEW METHOD: In contrast, this work proposes a mathematical framework for the analysis of randomness directly from functional connectivity assessments...
March 27, 2018: Journal of Neuroscience Methods
Tao Ruan, Lingjun Li, Yingnan Lyu, Qin Luo, Bangyuan Wu
The aim of this study was to investigate the effects of methionine (Met) deficiency on antioxidant functions (in the duodenal, jejunal and ileal mucosa) and apoptosis in the duodenum, jejunum and ileum of broiler chickens. A total of 120 one-day-old Cobb broilers were divided into two groups and fed a Metdeficient diet and a control diet, respectively, for six weeks. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), the ability to inhibit hydroxyl radicals, and glutathione (GSH) content were significantly decreased in the Met-deficient group compared to the control...
March 2018: Acta Veterinaria Hungarica
Melissa Van Bossuyt, Els Van Hoeck, Giuseppa Raitano, Tamara Vanhaecke, Emilio Benfenati, Birgit Mertens, Vera Rogiers
In silico methodologies, such as (quantitative) structure-activity relationships ((Q)SARs), are available to predict a wide variety of toxicological properties and biological activities for structurally diverse substances. To obtain insights in the scientific value of these predictions, the capacity of the prediction models to generate (sufficiently) reliable results for a particular type of compounds needs to be evaluated. In the current study, performance parameters to predict the endpoint 'bacterial mutagenicity' were calculated for a battery of common (Q)SAR tools, namely Toxtree, Derek Nexus, VEGA Consensus and Sarah Nexus...
March 20, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Yong Zhao, Chun-Hua Ding
Background: Recent research indicates that nerve growth factor (NGF) promotes cardiac repair following myocardial infarction by promoting angiogenesis and cardiomyocyte survival. The purpose of this study was to investigate the effects of NGF on cardiac fibroblasts (CFs) proliferation, cell cycle, migration, and myofibroblast transformation in vitro. Methods: CFs were obtained from ventricles of neonatal Sprague-Dawley rats and incubated with various concentrations of NGF (0, 0...
April 5, 2018: Chinese Medical Journal
Edwin van der Pol, Auguste Sturk, Ton van Leeuwen, Rienk Nieuwland, Frank Coumans
BACKGROUND: Detection of extracellular vesicles (EVs) by flow cytometry (FCM) has poor inter-laboratory comparability due to differences in FCM sensitivity. Previous workshops distributed polystyrene beads to set a scatter-based diameter gate to improve the comparability of EV concentration measurements. However, polystyrene beads provide limited insight into the diameter of detected EVs. OBJECTIVES: To evaluate gates based on the estimated diameter of EVs instead of beads...
March 25, 2018: Journal of Thrombosis and Haemostasis: JTH
Sandy Chan, Kristjan Pullerits, Janine Riechelmann, Kenneth M Persson, Peter Rådström, Catherine J Paul
While slow sand filters (SSFs) have produced drinking water for more than a hundred years, understanding of their associated microbial communities is limited. In this study, bacteria in influent and effluent water from full-scale SSFs were explored using flow cytometry (FCM) with cytometric histogram image comparison (CHIC) analysis; and routine microbial counts for heterotrophs, total coliforms and Escherichia coli. To assess if FCM can monitor biofilm function, SSFs differing in age and sand composition were compared...
March 13, 2018: Water Research
R Poongodi, Neelam Varma, Shano Naseem, Bose Parveen, Subhash Varma
Background: Chronic lymphoproliferative disorders (CLPDs) are heterogeneous group of disorders with variable clinical presentations and outcomes. Therefore, accurate classification is crucial for treatment planning. At present, flow cytometry immunophenotyping (FCM-IPT) is a useful tool for diagnosing these diseases. However, overlapping immunophenotypes do exist. Recently, differential expression of CD200 and variation in number of CD20 antibody bound per cell (ABC) in different CLPDs has been reported...
January 2018: Indian Journal of Pathology & Microbiology
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