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Francesco Danza, Nicola Storelli, Samuele Roman, Samuel Lüdin, Mauro Tonolla
The meromictic Lake Cadagno is characterized by a compact chemocline with high concentrations of anoxygenic phototrophic purple sulfur bacteria (PSB) and green sulfur bacteria (GSB). The co-occurrence of phylogenetically distant bacterial groups such as PSB and GSB in the same ecological niche, makes the chemocline of Lake Cadagno an ideal system for studying the conditions and consequences of coexistence of photosynthetic bacteria populations. In this study, we applied flow cytometry (FCM) as a fast tool to identify metabolic changes due to the production and consumption of inclusion bodies such as sulfur globules (SGBs), and follow population dynamics of closely related anoxygenic photosynthetic sulfur bacteria in their natural environment...
2017: PloS One
Chun-Li Liu, Jing-Yi Cai, Hao-Ran Bi, Tian-Wei Tan
High-throughput screening is a popular tool for collating biological data which would otherwise require the use of excessive resources. In this study, an artificial genetic circuit sensor responding to dimethylallyl diphosphate (DMAPP) was constructed based on a modified L-arabinose operon for high-throughput screening and isoprene synthase (ispS) evolution in Escherichia coli (E. coli). As a first step, the DNA sequence of the L-arabinose ligand-binding domain (LBD) was replaced with an ispS gene to enable the AraC operon responding to DMAPP, which is the substrate of the IspS enzyme...
December 14, 2017: Applied Microbiology and Biotechnology
Xiaoyuan Gong, Dong Lin, Huijun Wang, Ying Wang, Bingcheng Liu, Hui Wei, Chunlin Zhou, Kaiqi Liu, Shuning Wei, Benfa Gong, Guangji Zhang, Yuntao Liu, Xingli Zhao, Yan Li, Runxia Gu, Shaowei Qiu, Yingchang Mi, Jianxiang Wang
To explore the value of flow cytometric (FCM) analysis of cerebrospinal fluid (CSF) in the diagnosis of central nervous system involvement in adult patients with acute lymphoblastic leukemia (ALL) during follow-up METHODS: 2871 CSF samples from 357 adult patients with newly diagnosed ALL between the year of 2009 and 2015 were analysed retrospectively. These patients were divided into 3 groups according to CSF results, FCM+/conventional cytology (CC)+ group, FCM+/CC- group, and FCM-/CC- group, respectively. The overall survival (OS) of the three groups was analysed RESULTS: Fifteen (4...
December 14, 2017: European Journal of Haematology
Jorge E Toblli, Gabriel Cao, Luis Rico, Margarita Angerosa
Background: Ferric carboxymaltose (FCM) is a stable, non-dextran-based intravenous iron complex used to treat iron deficiency of various etiologies. As FCM is a nonbiological complex drug and cannot be fully characterized by physicochemical analyses, it is important to demonstrate in nonclinical models that FCM similars (FCMS) have similar biodistribution. Materials and methods: A total of 30 nonanemic rats were treated weekly with 40 mg iron/kg body weight intravenous FCM, FCMS, or isotonic saline (controls) for 4 weeks...
2017: Drug Design, Development and Therapy
Xingyu Wang, Anning Gao, Yadong Jiao, Yan Zhao, Xingbin Yang
Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study. The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity. The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31...
December 9, 2017: International Journal of Biological Macromolecules
Fang Miao, Xueshan Zhang, Yanning Cao, Yue Wang, Xiaoshu Zhang
BACKGROUND: The role of Spalt-like gene-2 (SALL2) in tumorigenesis remains incompletely elucidated. This study investigated the effects of SALL2 on human ovarian carcinoma (OC) A2780 cells and the probable mechanism. METHODS: Expression of SALL2 in human OC cell lines were detected by reverse transcription PCR (RT-PCR) and Western blot analysis. A2780 cells were transfected with small-interfering ribonucleic acid (siRNA) to silence SALL2. SALL2 expression was detected by RT-PCR, Western blot analysis and immunofluorescence assay...
December 11, 2017: BMC Cancer
Mianqiang Xue, Liang Zhou, Naoya Kojima, Leticia Sarmento Dos Muchangos, Takashi Machimura, Akihiro Tokai
Increasing manufacture and usage of chemicals have not been matched by the increase in our understanding of their risks. Pollutant release and transfer register (PRTR) is becoming a popular measure for collecting chemical data and enhancing the public right to know. However, these data are usually in high dimensionality which restricts their wider use. The present study partitions Japanese PRTR chemicals into five fuzzy clusters by fuzzy c-mean clustering (FCM) to explore the implicit information. Each chemical with membership degrees belongs to each cluster...
December 8, 2017: Science of the Total Environment
Jianhua Chen, Feng Liu
BACKGROUND: How to choose an appropriate method to monitoring dynamic ABO chimera post ABO incompatible HSCT is crucial to not only assess the status of erythroid engraftment but achieve personalized safety transfusion. METHODS: We evaluated the efficacy of micro gel column technique(MGCT) and flow cytometry(FCM) by series of artificial ABO chimera mixtures from 0.5% to 50% and by investigated 15 cases of ABO incompatible HSCT patients with longitudinally ABO blood grouping and ABO chimera from RBCs and reticulocytes...
December 11, 2017: Clinical Transplantation
Fabrian Brenz, Susanne Linke, Thomas Simat
Polybutylene terephthalate (PBT) is a polyester (PES) gaining more importance on the food contact material (FCM) market. However, little is known about the potential migration of PBT oligomers which are formed during the polymer production. In this work, PBT pellets and a slotted spoon manufactured from this material by injection moulding were analysed on extractable oligomers and their migration potential into hydrophilic foods. Overall 27 oligomers (cycles and linears) could be identified in different extracts by HPLC-DAD/ESI-MS data, but without confirmation by reference substances...
December 11, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Eelco N Pieke, Jørn Smedsgaard, Kit Granby
In fields such as food safety and environmental chemistry, ensuring safety is greatly challenged by large numbers of unknown substances occurring. Even with current state of the art mass spectrometers, dealing with non-identified substances is a very laborious process as it includes structure elucidation of a vast number of unknowns, of which only a fraction may be relevant. Here, we present an exploration and prioritization approach based on high resolution mass spectrometry. The method uses algorithm-based precursor/product-ion correlations on Quadrupole-Time of Flight (Q-TOF) MS/MS data to retrieve the most likely chemical match from a structure database...
December 8, 2017: Journal of Mass Spectrometry: JMS
You Sun, Feifei Wang, Xuehui Sun, Xiaoxue Wang, Lei Zhang, Yang Li
OBJECTIVE: The study was aimed to investigate the impact of CX3CR1 expression on the proliferation and apoptosis of osteoarthrosis (OA) chondrocytes through Wnt/ β-catenin pathway. METHODS: The expression levels of CX3CR1 and Wnt/β-catenin pathway-related genes mRNA and protein in OA chondrocytes were examined by qRT-PCR and western blot. MTT and flow cytometry (FCM) assays were employed to assess cell proliferation, cell cycle and apoptosis. XAV-939, a Wnt/β-catenin pathway inhibitor, was used to inhibit the pathway...
December 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Li Wang, Dequan Liu, Xingrao Wu, Yueqin Zeng, Lan Li, Yu Hou, Wenhui Li, Zhijie Liu
OBJECTIVE: Microarray showed that lncRNA RMST was differentially expressed in cervical cancer. Further experiments were conducted to detect the expression and biological function of RMST in triple-negative breast cancer (TNBC). METHODS: Microarray was used to screen the differentially expressed lncRNAs in TNBC. QRT-PCR was applied to uncover the expression of RMST in TNBC tissues. The cell viability of RMST-transfected TNBC cells were probed by CKK-8 assay and colony formation assay...
December 7, 2017: Journal of Cellular Physiology
Hai Song, Shanhua Fang, Jing Gao, Jiaxong Wang, Zhenzhen Cao, Zeyun Guo, Qiongping Huang, Yongqang Qu, Hu Zhou, Jianyun Yu
Traumatic brain injury (TBI), as a neurological injury, becomes a leading cause of disability and mortality due to lacking effective therapy. About 75% of TBI is mild traumatic brain injury (mTBI). However, the complex molecular mechanisms underlying mTBI pathophysiology remains to be elucidated. In this study, iTRAQ-based quantitative proteomic approach was employed to measure temporal-global proteome changes of rat brain tissues from different time points (1 day, 7 day and 6 months) post single mTBI (smTBI) and repetitive mTBI (rmTBI)...
December 7, 2017: Journal of Proteome Research
Michael D Besmer, Frederik Hammes, Jürg A Sigrist, Christoph Ort
Monitoring of microbial drinking water quality is a key component for ensuring safety and understanding risk, but conventional monitoring strategies are typically based on low sampling frequencies (e.g., quarterly or monthly). This is of concern because many drinking water sources, such as karstic springs are often subject to changes in bacterial concentrations on much shorter time scales (e.g., hours to days), for example after precipitation events. Microbial contamination events are crucial from a risk assessment perspective and should therefore be targeted by monitoring strategies to establish both the frequency of their occurrence and the magnitude of bacterial peak concentrations...
2017: Frontiers in Microbiology
Hala M Abaza, Mervat A A Alfeky, Deena S Eissa, Mona F Abdel Fattah, Laila M Annaka, Fatma S Ebeid
Flow cytometry (FCM) is used for quantification of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) through discriminating leukemic B-lymphoblasts from normal B-cell precursor counterparts "hematogones." Neuropilin-1 (NRP-1)/CD304 is a vascular endothelial growth factor receptor implicated in the progression of hematological malignancies. We evaluated NRP-1/CD304 as MRD and prognostic marker in pediatric precursor B-ALL using FCM. Seventy children with precursor B-ALL and 40 control children were enrolled...
December 1, 2017: Journal of Pediatric Hematology/oncology
Dan Wu, Mulin Liang, Hongxing Dang, Fang Fang Feng Xu, Chengjun Liu
Oxidative stress is regarded as a key regulator in the pathogenesis of prolonged hyperoxia-induced lung injury, which causes injury to alveolar epithelial cells and eventually leads to development of bronchopulmonary dysplasia (BPD). Many studies have shown that hydrogen has a protective effect in a variety of cells. However, the mechanisms by which hydrogen rescues cells from damage due to oxidative stress in BPD remains to be fully elucidated. This study sought to evaluate the effects of hydrogen on hyperoxia-induced lung injury and to investigate the underlying mechanism...
November 30, 2017: Biochemical and Biophysical Research Communications
Christina A Banzhaf, Lynlee L Lin, Nhung Dang, Michael Freeman, Merete Haedersdal, Tarl W Prow
BACKGROUND: Ablative fractional laser (AFXL) is an acknowledged technique to increase uptake of topical agents in skin. Micro thermal ablation zones (MAZs) consist of ablated vertical channels surrounded by a coagulation zone (CZ). Laser scanning confocal microscopy (LSCM) images individual MAZs at 733 nm (reflectance confocal microscopy (RCM)). Further, LSCM can image sodium fluorescein (NaF) fluorescence with 488 nm excitation (fluorescence confocal microcopy (FCM)), a small hydrophilic test molecule (370 MW, log P -1...
November 29, 2017: Lasers in Surgery and Medicine
Ling Xie, Xiaohong Zhong, Dongbo Liu, Lin Liu, Zhilan Xia
BACKGROUND: Conventional scientific studies had supported the use of polysaccharides and β-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. METHODS: A Sprague-Dawley (SD) rat model with ROU was established by autoantigen injection. The ROU rats were treated with three different dosages of FDPGLM and prednisone acetate (PA), and their effects were evaluated according to the clinical therapeutic evaluation indices of ROU...
December 1, 2017: BMC Complementary and Alternative Medicine
Kathy Coufal, Douglas Parham, Melissa Jakubowitz, Cassandra Howell, Jared Reyes
Purpose: Using American Speech-Language-Hearing Association's (ASHA's) National Outcomes Measurement System (NOMS) Functional Communication Measure (FCM) as a common metric, this investigation compared traditional service delivery and telepractice service delivery for children receiving therapy for the NOMS diagnostic category of "speech sound production." Method: De-identified cases were secured from ASHA's NOMS database and a proprietary database from a private e-learning provider...
November 29, 2017: American Journal of Speech-language Pathology
Jie Tang, Ce Chen, Cheng Zha, Jian-Rong Chang, Qiang Fang, Zhao-Hua Wang, Bai-Qing Li
OBJECTIVE: To investigate the effects of low molecular peptide of Mycobacterium tuberculosis heat-resistant antigen (Mtb-HAg-10k) on the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood T cells and test the feasibility of differential diagnosis between pulmonary tuberculosis (PTB) and latent tuberculosis infection (LTBI) by assessing the number of Mtb-HAg-10k-stimulated IFN-γ-producing T cells. METHODS: Peripheral blood mononuclear cells (PBMCs) were separated from the peripheral blood of 10 healthy adults, 6 individuals with LTBI and 13 patients with PTB...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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