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https://www.readbyqxmd.com/read/28730944/integrating-bioassays-and-analytical-chemistry-as-an-improved-approach-to-support-safety-assessment-of-food-contact-materials
#1
Julien Veyrand, Maricel Marin-Kuan, Claudine Bezencon, Nancy Frank, Violaine Guérin, Sander Koster, Hélia Latado, Julie Mollergues, Amaury Patin, Dominique Piguet, Patrick Serrant, Jesus Varela, Benoît Schilter
Food contact materials (FCM) contain chemicals which can migrate into food and result in human exposure. Although it is mandatory to ensure that migration does not endanger human health, there is still no consensus on how to pragmatically assess the safety of FCM since traditional approaches would require extensive toxicological and analytical testing which are expensive and time consuming. Recently, the combination of bioassays, analytical chemistry and risk assessment has been promoted as a new paradigm to identify toxicologically relevant molecules and address safety issues...
July 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28727075/amino-acid-digestibility-by-weanling-pigs-of-processed-ingredients-originating-from-soybeans-00-rapeseeds-or-a-fermented-mixture-of-plant-ingredients
#2
D M D L Navarro, Y Liu, T S Bruun, H H Stein
An experiment was conducted to determine the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of CP and AA in 4 sources of processed soybean products, in conventional dehulled soybean meal (SBM-CV), in conventional 00-rapeseed expellers (RSE), and in a fermented coproduct mixture (FCM) that contained rapeseed meal, wheat, soy molasses, and potato peel fed to weanling pigs. The 4 processed soybean products included 2 sources of enzyme-treated soybean meal (ESBM-1 and ESBM-2), extruded soybean meal, and soy protein concentrate (SPC)...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28724216/the-extract-from-punica-granatum-pomegranate-peel-induces-apoptosis-and-impairs-metastasis-in-prostate-cancer-cells
#3
Yuanle Deng, Yali Li, Fangfang Yang, Anqi Zeng, Shuping Yang, Yi Luo, Yiwen Zhang, Yongmei Xie, Tinghong Ye, Yong Xia, Wenya Yin
Prostate cancer is a big threat to male for its poor prognosis and high mortality rate. Natural compounds are important resources of many anticancer drugs. Pomegranate is a kind of antioxidant-rich fruit and its peel and seed has potential anticancer activities. In this study, we aimed to investigate the effects of pomegranate peel extract (PoPx) on the apoptosis and metastasis of prostate cancer cells and the related mechanism. We found that PoPx showed growth inhibition on prostate cancer cells. Nuclei morphological and flow cytometer (FCM) analysis indicated that PoPx could induce prostate cancer apoptosis...
July 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28719077/metformin-synergistic-pemetrexed-suppresses-non-small-cell-lung-cancer-cell-proliferation-and-invasion-in-vitro
#4
Yan Zhang, Xiuli Feng, Tao Li, Erpan Yi, Yu Li
The aim of this study was to investigate whether metformin in combination with pemetrexed has an effect on the treatment of non-small-cell lung cancer (NSCLC) models and to explore the related molecular mechanism. The half maximal inhibitory concentration (IC50) and combination index (CI) of metformin and pemetrexed were detected by the CCK8 assay to assess the antiproliferative and therapeutic effects of the two-drug combination. Flow cytometry (FCM) and invasion assays were used to estimate the variation in apoptosis rate and invasion ability of the differently treated NSCLC cell lines...
July 18, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28718199/the-clec12a-receptor-marks-human-basophils-potential-implications-for-minimal-residual-disease-detection-in-myeloid-malignancies
#5
Marie Toft-Petersen, Anne Stidsholt Roug, Trine Plesner, Lene Ebbesen, Gordon D Brown, Line Nederby
BACKGROUND: The transmembrane receptor C-type lectin domain family 12, member A (CLEC12A) is known to be highly expressed on monocytes and neutrophils and is a reliable leukemia associated marker in acute myeloid leukemia. Consequently, detailed knowledge of the various normal cell types expressing this receptor is essential. We have observed CLEC12A to be expressed on CD45lowSSClowCD14-CD123+ basophils in peripheral blood (PB) and in the present study, we aimed at verifying this observation and further delineate the CD45lowSSClowCD14-CD123+CLEC12A+ subpopulation...
July 17, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28706363/comparative-study-of-efficacy-and-safety-of-ferric-carboxymaltose-versus-iron-sucrose-in-post-partum-anaemia
#6
Nalini Sharma, J Lalnunnem Thiek, Tanie Natung, Santa Singh Ahanthem
BACKGROUND: The incidence of post-partum anaemia (PPA) is 14-24%. Treatment of PPA with injectable iron replenishes the iron store. Ferric carboxymaltose complex (FCM) is a non-dextran containing intravenous iron agent, having a very low immunogenic potential, designed to be administered in large doses in a short period of time. OBJECTIVE: To compare the efficacy and safety of intravenous FCM and iron sucrose (IS) in post-partum iron-deficiency anaemia. MATERIAL AND METHOD: In this prospective, comparative study, 120 post-partum women with iron-deficiency anaemia (Hb < 10 g%) were divided into two groups...
August 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28705533/characterization-and-prediction-of-the-backscattered-form-function-of-an-immersed-cylindrical-shell-using-hybrid-fuzzy-clustering-and-bio-inspired-algorithms
#7
Said Agounad, El Houcein Aassif, Younes Khandouch, Gérard Maze, Dominique Décultot
The acoustic scattering of a plane wave by an elastic cylindrical shell is studied. A new approach is developed to predict the form function of an immersed cylindrical shell of the radius ratio b/a ('b' is the inner radius and 'a' is the outer radius). The prediction of the backscattered form function is investigated by a combined approach between fuzzy clustering algorithms and bio-inspired algorithms. Four famous fuzzy clustering algorithms: the fuzzy c-means (FCM), the Gustafson-Kessel algorithm (GK), the fuzzy c-regression model (FCRM) and the Gath-Geva algorithm (GG) are combined with particle swarm optimization and genetic algorithm...
June 20, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28704473/enriched-environment-and-stress-exposure-influence-splenic-b-lymphocyte-composition
#8
Blake T Gurfein, Burcu Hasdemir, Jeffrey M Milush, Chadi Touma, Rupert Palme, Douglas F Nixon, Nicholas Darcel, Frederick M Hecht, Aditi Bhargava
Prolonged chronic stress has deleterious effects on immune function and is associated with numerous negative health outcomes. The spleen harbors one-fourth of the body's lymphocytes and mediates both innate and adaptive immune responses. However, the subset of splenic lymphocytes that respond, either adaptively or maladaptively, to various stressors remains largely unknown. Here we investigated the effects of unpredictable chronic mild stress (CMS) exposure on spleen composition in male mice housed in two different caging conditions: standard caging (Cntl) and enriched environment (EE)...
2017: PloS One
https://www.readbyqxmd.com/read/28701470/effect-of-ferric-carboxymaltose-on-exercise-capacity-in-patients-with-chronic-heart-failure-and-iron-deficiency
#9
Dirk J van Veldhuisen, Piotr Ponikowski, Peter van der Meer, Marco Metra, Michael Böhm, Artem Doletsky, Adriaan A Voors, Iain C Macdougall, Stefan D Anker, Bernard Roubert, Lorraine Zakin, Alain Cohen-Solal
Background -Iron deficiency is common in patients with heart failure (HF), and is associated with reduced exercise capacity and poor outcomes. Whether correction of iron deficiency with (intravenous) ferric carboxymaltose (FCM) affects peak oxygen consumption [peak VO2], an objective measure of exercise intolerance in HF, has not been examined. Methods -We studied patients with systolic HF (left ventricular ejection fraction [LVEF] ≤45%) and mild to moderate symptoms despite optimal HF medication, Patients were randomized 1:1 to treatment with FCM for 24 weeks or standard of care...
July 12, 2017: Circulation
https://www.readbyqxmd.com/read/28684789/soil-bacterial-quantification-approaches-coupling-with-relative-abundances-reflecting-the-changes-of-taxa
#10
Zhaojing Zhang, Yuanyuan Qu, Shuzhen Li, Kai Feng, Shang Wang, Weiwei Cai, Yuting Liang, Hui Li, Meiying Xu, Huaqun Yin, Ye Deng
Understanding the abundance change of certain bacterial taxa is quite important for the study of soil microbiology. However, the observed differences of relative abundances by high-throughput techniques may not accurately reflect those of the actual taxon abundances. This study investigated whether soil microbial abundances coupling with microbial quantities can be more informative in describing the microbial population distribution under different locations. We analyzed relative abundances of the major species in soil microbial communities from Beijing and Tibet grasslands by using 16 S rRNA high-throughput sequencing technique, and quantified the absolute bacterial cell numbers directly or indirectly by multiple culture-independent measurements, including adenosine tri-phosphate (ATP), flow cytometry (FCM), quantitative real-time PCR (qPCR), phospholipid fatty acids (PLFA) and microbial biomass Carbon (MBC)...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28682874/in-vitro-effect-of-microrna-107-targeting-dkk-1-by-regulation-of-wnt-%C3%AE-catenin-signaling-pathway-in-osteosarcoma
#11
Zhi-Cai Zhang, Jian-Xiang Liu, Zeng-Wu Shao, Fei-Fei Pu, Bai-Chuan Wang, Qiang Wu, Yu-Kun Zhang, Xian-Lin Zeng, Xiao-Dong Guo, Shu-Hua Yang, Tong-Chuan He
BACKGROUND: The aim of the study was to explore the effects of microRNA-107 (miR-107) by targeting Dkk-1 on osteosarcoma (OS) via the Wnt/β-catenin signaling pathway. METHODS: OS and adjacent tissues were collected from 67 patients diagnosed with OS. Expressions of miR-107, Dkk-1, LRP5, β-catenin, and c-Myc were detected by the quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. The dual-luciferase reporter gene assay was performed to observe the relationship between miR-107 and Dkk-1...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28681074/statistical-clustering-of-parametric-maps-from-dynamic-contrast-enhanced-mri-and-an-associated-decision-tree-model-for-non-invasive-tumour-grading-of-t1b-solid-clear-cell-renal-cell-carcinoma
#12
Yin Xi, Qing Yuan, Yue Zhang, Ananth J Madhuranthakam, Michael Fulkerson, Vitaly Margulis, James Brugarolas, Payal Kapur, Jeffrey A Cadeddu, Ivan Pedrosa
OBJECTIVES: To apply a statistical clustering algorithm to combine information from dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) into a single tumour map to distinguish high-grade from low-grade T1b clear cell renal cell carcinoma (ccRCC). METHODS: This prospective, Institutional Review Board -approved, Health Insurance Portability and Accountability Act -compliant study included 18 patients with solid T1b ccRCC who underwent pre-surgical DCE MRI...
July 5, 2017: European Radiology
https://www.readbyqxmd.com/read/28678686/cross-species-antiviral-activity-of-goose-interferon-lambda-against-duck-plague-virus-is-related-to-its-positive-self-regulatory-feedback-loop
#13
Shun Chen, Wei Zhang, Qin Zhou, Anqi Wang, Lipei Sun, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen, Anchun Cheng
Duck plague virus (DPV) is a virus of the Herpesviridae family that leads to acute disease with a high mortality rate in ducks. Control of the disease contributes to the development of poultry breeding. Type III IFN family (IFN-λs) is a novel member of the IFN family, and goose IFN-λ (goIFN-λ) is a newly identified gene whose antiviral function has only been investigated to a limited extent. Here, the cross-species antiviral activity of goIFN-λ against DPV in duck embryo fibroblasts (DEFs) was studied. We found that pre-treatment with goIFN-λ greatly increased the expression of IFN-λ in both heterologous DEFs and homologous goose embryo fibroblasts (GEFs), while differentially inducing IFNα- and IFN-stimulated genes...
June 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28673331/tmpyp4-exerted-antitumor-effects-in-human-cervical-cancer-cells-through-activation-of-p38-mitogen-activated-protein-kinase
#14
Ming-Jun Cheng, Yun-Gui Cao
BACKGROUND: The aim of the present study was to investigate the potential effects of the 5,10,15,20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions. RESULTS: After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen-activated protein kinase (MAPK), phosphated p38 MAPK (p-p38 MAPK), capase-3, MAPKAPK2 (MK-2) and poly ADP-ribose polymerase (PARP) was measured by Western blot analysis...
July 3, 2017: Biological Research
https://www.readbyqxmd.com/read/28669772/naoxintong-inhibits-myocardial-infarction-injury-by-vegf-enos-signaling-mediated-neovascularization
#15
Hong Wang, Lizhen Qiu, Yake Ma, Lusha Zhang, Lu Chen, Chunxiao Li, Xiao Geng, Xingyu You, Xiumei Gao
ETHNOPHARMACOLOGICAL RELEVANCE: Naoxintong capsules (NXT), a traditional Chinese Medical preparation, are widely used for treatment of cardiovascular diseases, while the mechanism is still unclear. MATERIALS AND METHODS: Myocardial infarction (MI) was induced by ligation of the left coronary artery in mice. Echocardiographic measurements were performed to do physiological assessments of left ventricle (LV) function. Histological and immunohistochemical staining was used to determine infarct size, capillary density, tissue endothelial nitric oxide synthase (eNOS) expression...
June 29, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28669173/flow-cytometric-analysis-of-dna-ploidy-in-liquid-based-cytology-for-cervical-pre-cancer-and-cancer
#16
Sridhar Mishra, Namrata P Awasthi, Nuzhat Husain, Akansha Anand, Yashodhara Pradeep, Roohi Ansari, Sarita Saxena
Background: DNA ploidy analysis of cervical intraepithelial neoplasia (CIN) and invasive cervical cancer samples by flow cytometry (FCM) has been established as an aid to prognostic assessment. Liquid based cytology (LBC) increases diagnostic specificity by using ancillary techniques that provide information beyond morphology. The present study was undertaken to assess DNA ploidy in LBC samples as an adjunct for early detection of cervical pre-cancer and cancer. Methods: DNA ploidy assessment was performed on LBC samples of 50 cases and 31 controls...
June 25, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28650331/expression-of-th17-and-cd4-cd25-t-regulatory-cells-in-peripheral-blood-of-acute-leukemia-patients-and-their-prognostic-significance
#17
Mingli Xiang, Li Guo, Yan Ma, Yi Li
To discuss the expression of T helper cell 17 (Th17) cells and CD4+ CD25+ Foxp3+ regulatory T cells (Treg) in peripheral blood (PB) of patients with acute leukemia (AL), and to explore the relationship between them and disease prognosis. 40 patients diagnosed with acute leukemia in The First Affiliated Hospital of Zhengzhou University from July 2012 to August 2014 were selected as the observation group. Meanwhile, 40 healthy people were taken as the control group. Flow Cytometry Method (FCM) was used to detect the level of Th17 cells and CD4(+) CD25(+) Foxp3(+) cells in peripheral blood of the two groups, and enzyme-linked immuno sorbent assay (ELISA) method was used to test the level of IL17 and TGF-β in peripheral blood of two groups; reverse transcription-polymerase chain reaction (RT-PCR) was adopted to analyze the mRNA levels of RORγT and Foxp3 in peripheral blood...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28648946/end-of-therapy-minimal-residual-disease-mrd-measurement-in-children-with-all-does-not-predict-relapse
#18
Tahani Hani Sarrawi, Ismael Zayyat, Fareed Barakat, Maha Rezeq, Salam Abu Jmaian, Faris Madanat
Minimal residual disease (MRD) monitoring opened a new era for childhood acute lymphoblastic leukemia (ALL), and is widely used for risk adapted therapy in the major study groups (Borowitz et al. 2015; Eckert et al., 2015; Peregud-Pogorzelsk et al., 2003). MRD is measured either by polymerase chain reaction (PCR) or flow cytometry (FCM) (Campana, 2009). Most studies have concentrated on measuring MRD during or at end of induction, and after consolidation therapy, to stratify patients and to predict relapse (Sutton et al...
June 17, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28643901/ferric-carboxymaltose-in-patients-with-restless-legs-syndrome-and-nonanemic-iron-deficiency-a-randomized-trial
#19
Claudia Trenkwalder, Juliane Winkelmann, Wolfgang Oertel, Garth Virgin, Bernard Roubert, Anna Mezzacasa
BACKGROUND: Compromised iron status is important in restless legs syndrome pathophysiology. We compared the efficacy and tolerability of ferric carboxymaltose (single intravenous dose) versus placebo for restless legs syndrome treatment in iron-deficient nonanemic patients. METHODS: Patients with moderate to severe restless legs syndrome and serum ferritin < 75 μg/L (or serum ferritin 75-300 μg/L and transferrin saturation < 20%) were randomized to ferric carboxymaltose (1000 mg iron) or placebo...
June 23, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/28641652/-significance-of-monitoring-minimal-residual-disease-by-flow-cytometry-in-acute-leukemia-patients-underwent-nonmyeloablative-allo-hsct
#20
Xiong-Xiong Liu, Tie-Qiang Liu, Mei Guo, Qi-Yun Sun, Jian-Hui Qiao, Kai-Xun Hu, Bing-Xia Li, Bo Yao, Chang-Lin Yu
OBJECTIVE: To explore the value of dynamically monitoring minimal residual disease (MRD) by flow cytometry before and after non-myeloablative allo-HSCT (NST) for prediction of acute leukemia(AL) relapse after transplantation. METHODS: The clinical data of 51 AL patients underwent NST were analyzed retrospectively in Department of Hematology of Affiliated Hospital of Academy of Military Medical Sciences from January 2011 to December 2015. All AL patients achieved the morphologic complete remission of bone marrow before transplantation...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
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