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https://www.readbyqxmd.com/read/28810608/mechanism-of-microrna-21-regulating-il-6-inflammatory-response-and-cell-autophagy-in-intervertebral-disc-degeneration
#1
Hao Lin, Wenzhi Zhang, Tao Zhou, Wansu Li, Ziao Chen, Chaochao Ji, Chao Zhang, Fang He
This study investigated the mechanism of microRNA-21 in regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. A total of 10 patients with lumbar disc herniation accompanied by nerve root pain (observation group) and 10 patients with lumbar burst fractures (control group) were selected. The nucleus pulposus tissues of the lesion were obtained during operation for cell culture. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of microRNA-21...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810568/mechanism-of-microrna-146a-mediated-il-6-stat3-signaling-in-lumbar-intervertebral-disc-degeneration
#2
Tao Zhou, Hao Lin, Ziao Cheng, Chaochao Ji, Chao Zhang, Jiwei Tian
The aim of the study was to investigate the mechanism of microRNA (miR)-146a-mediated activation of interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) in lumbar intervertebral disc degeneration. To obtain intervertebral tissue, we recruited 5 patients with lumbar intervertebral disc herniation (experimental group) and 5 patients with lumbar burst fracture (control group). Nucleus pulposus tissue was extracted by surgery and cultured. miR-146a empty vector, mimic, and inhibitor were transfected into the two groups of cells for 24 h and the levels of IL-6, type II collagen (Col II), aggrecan, STAT3, matrix metalloproteinase (MMP)-3, and a disintegrin and metalloproteinase with thrombospondin type I motifs (ADAMTS) were detected...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28761373/chronic-constriction-injury-of-sciatic-nerve-changes-circular-rna-expression-in-rat-spinal-dorsal-horn
#3
Song Cao, Wenwen Deng, Ying Li, Bangyong Qin, Lin Zhang, Shouyang Yu, Peng Xie, Zhi Xiao, Tian Yu
BACKGROUND: Mechanisms of neuropathic pain are still largely unknown. Molecular changes in spinal dorsal horn may contribute to the initiation and development of neuropathic pain. Circular RNAs (circRNAs) have been identified as microRNA sponges and involved in various biological processes, but whether their expression profile changes in neuropathic pain condition is not reported. METHODS: To test whether neuropathic pain influences circRNA expression, we developed a sciatic chronic constriction injury (CCI) model in rats...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28739526/chronic-mild-stress-exacerbates-severity-of-experimental-autoimmune-encephalomyelitis-in-association-with-altered-non-coding-rna-and-metabolic-biomarkers
#4
Brietta Gerrard, Vaibhav Singh, Olena Babenko, Isabelle Gauthier, V Wee Yong, Igor Kovalchuk, Artur Luczak, Gerlinde A S Metz
The causal factors determining the onset and severity of multiple sclerosis (MS) are not well understood. Here, we investigated the influence of chronic stress on clinical symptoms, metabolic and epigenetic manifestations of experimental autoimmune encephalomyelitis (EAE), a common animal model of MS. Lewis rats were immunized for monophasic EAE with MBP69-88 and were exposed to chronic stress for 37days starting 7days prior to immunization. The exposure to stress accelerated and exacerbated the clinical symptoms of EAE...
July 21, 2017: Neuroscience
https://www.readbyqxmd.com/read/28669725/mir-21-promotes-fibrosis-and-hypertrophy-of-ligamentum-flavum-in-lumbar-spinal-canal-stenosis-by-activating-il-6-expression
#5
Chao Sun, Jiwei Tian, Xinhui Liu, Guoping Guan
The molecular mechanism underlying the fibrosis of ligamentum flavum(LF) in patients with lumbar spinal canal stenosis(LSCS) remains unknown. MicroRNAs are reported to play important roles in regulating fibrosis in different organs. The present study aimed to identify fibrosis related miR-21 expression profile and investigate the pathological process of miR-21 in the fibrosis of LF hypertrophy and associated regulatory mechanisms. 15 patients with LSCS underwent surgical treatment were enrolled in this study...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28623098/acquired-inhibition-of-microrna-124-protects-against-spinal-cord-ischemia-reperfusion-injury-partially-through-a-mitophagy-dependent-pathway
#6
Kun Liu, Lihui Yan, Xiaojing Jiang, Yang Yu, Hongbo Liu, Tianxiang Gu, Enyi Shi
OBJECTIVE: Mitophagy results in selective clearance of damaged mitochondria. We investigated whether mitophagy was involved in the neuroprotection by inhibiting microRNA (miRNA)-124 on ischemic spinal cords. METHODS: Inhibition of miRNA-124 was conducted by intrathecal injection of lentivirus vectors containing antagomiR-124. Spinal cord ischemia was induced in rats by crossclamping the descending aorta just distal to the left subclavian artery for 14 minutes. Hind-limb motor function was assessed with the motor deficit index (MDI)...
May 23, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28611312/a-cerebrospinal-fluid-microrna-signature-as-biomarker-for-glioblastoma
#7
Johnny C Akers, Wei Hua, Hongying Li, Valya Ramakrishnan, Zixiao Yang, Kai Quan, Wei Zhu, Jie Li, Javier Figueroa, Brian R Hirshman, Brittney Miller, David Piccioni, Florian Ringel, Ricardo Komotar, Karen Messer, Douglas R Galasko, Fred Hochberg, Ying Mao, Bob S Carter, Clark C Chen
PURPOSE: To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma. EXPERIMENTAL DESIGN: Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts. RESULTS: miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues...
June 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28580822/effects-of-microrna-223-on-morphine-analgesic-tolerance-by-targeting-nlrp3-in-a-rat-model-of-neuropathic-pain
#8
Xiao-Juan Xie, Li-Gang Ma, Kai Xi, Dong-Mei Fan, Jian-Guo Li, Quan Zhang, Wei Zhang
Objective To investigate the effects of microRNA-223 on morphine analgesic tolerance by targeting NLRP3 in a rat model of neuropathic pain. Methods Our study selected 100 clean grade healthy Sprague-Dawley adult male rats weighing 200 to 250 g. After establishment of a rat model of chronic constriction injury, these rats were divided into 10 groups (10 rats in each group): the normal control, sham operation, chronic constriction injury, normal saline, morphine, miR-223, NLRP3, miR-223 + morphine, NLRP3 + morphine, and miR-223 + NLRP3 + morphine groups...
January 2017: Molecular Pain
https://www.readbyqxmd.com/read/28512472/pulsed-electromagnetic-field-regulates-microrna-21-expression-to-activate-tgf-%C3%AE-signaling-in-human-bone-marrow-stromal-cells-to-enhance-osteoblast-differentiation
#9
Nagarajan Selvamurugan, Zhiming He, Daniel Rifkin, Branka Dabovic, Nicola C Partridge
Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in nonunions and increase lumbar spinal fusion rates. However, the molecular mechanisms by which PEMF stimulates differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts are not well understood. In this study the PEMF effects on hBMSCs were studied by microarray analysis. PEMF stimulation of hBMSCs' cell numbers mainly affected genes of cell cycle regulation, cell structure, and growth receptors or kinase pathways...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28460630/microrna-223-demonstrated-experimentally-in-exosome-like-vesicles-is-associated-with-decreased-risk-of-persistent-pain-after-lumbar-disc-herniation
#10
Aurora Moen, Daniel Jacobsen, Santosh Phuyal, Anna Legfeldt, Fred Haugen, Cecilie Røe, Johannes Gjerstad
BACKGROUND: Previous findings have demonstrated that lumbar radicular pain after disc herniation may be associated with up-regulation of inflammatory mediators. In the present study we examined the possible role of extracellular microRNAs (miRs) in this process. METHODS: Single unit recordings, isolation of exosome-like vesicles, electron microscopy, nanoparticle tracking analysis, western blot analysis and qPCR were used in rats to demonstrate the effect of nucleus pulposus (NP) applied onto the dorsal nerve roots...
May 1, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28186136/foxo1-suppressed-mir-424-regulates-the-proliferation-and-osteogenic-differentiation-of-mscs-by-targeting-fgf2-under-oxidative-stress
#11
Liangping Li, Qihua Qi, Jiaquan Luo, Sheng Huang, Zemin Ling, Manman Gao, Zhiyu Zhou, Maik Stiehler, Xuenong Zou
Recently, microRNAs (miRNAs) have been identified as key regulators of the proliferation and differentiation of mesenchymal stem cells (MSCs). Our previous in vivo study and other in vitro studies using miRNA microarrays suggest that miR-424 is involved in the regulation of bone formation. However, the role and mechanism of miR-424 in bone formation still remain unknown. Here, we identified that the downregulation of miR-424 mediates bone formation under oxidative stress, and we explored its underlying mechanism...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27593550/overexpression-of-microrna-21-protects-spinal-cords-against-transient-ischemia
#12
Fang He, Yixing Ren, Enyi Shi, Kun Liu, Lihui Yan, Xiaojing Jiang
OBJECTIVE: MicroRNA (miRNA) molecules are involved in the response of the central nervous system to ischemia and reperfusion. We sought to test the hypothesis that overexpression of miRNA-21 can induce neuroprotection on spinal cords against transient ischemia. METHODS: Overexpression of miRNA-21 in vivo was conducted by means of intrathecal injection of lentivirus vectors containing pre-miRNA-21. The vehicle or control lentivirus vectors were given to the control group and the control vector group, respectively...
August 12, 2016: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/27347338/regulation-of-insulin-like-growth-factor-1-receptor-signaling-by-microrna-4458-in-the-development-of-lumbar-disc-degeneration
#13
Zuo-Qing Liu, Wen-Qin Fu, Shan Zhao, Xin Zhao
A potential role of Insulin-like growth factor 1 (IGF1) receptor/phosphatidylinositol-3 kinase (PI3k)/Akt signaling in the initiation and progression of Lumbar disc degeneration (LDD) has been recently reported. However, the regulation of IGF1 receptor (IGF1R) at post-transcriptional levels in the development of LDD remains unknown. Here, we studied the effects of microRNA-4458 on the expression of IGF1R. We examined the IGF1R levels and microRNA-4458 (miR-4458) levels in the resected LDD discs, compared with the traumatized, non-LDD discs...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/26987016/the-role-and-mechanisms-of-action-of-sirt6-in-the-suppression-of-postoperative-epidural-scar-formation
#14
Xiaochen Fan, Junjie Chen, Da Shi, Jing Jia, Jia He, Liang Li, Tao Lei, Xuesong Chen
In order to investigate the role which sirtuin-6 (SIRT6) plays in lumbar spinal epidural fibrosis (EF) and scar formation in vitro and in vivo, SIRT6 and transforming growth factor β (TGF‑β) protein levels in the lumbar disc of patients were detected using western blotting in patients who had undergone a laminectomy. The results demonstrated that SIRT6 expression was significantly reduced in the lumbar discs of patients in whom an epidural scar had formed, but the expression pattern of TGF-β2 was much higher...
May 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/26981236/mirna-expression-profiles-in-cerebrospinal-fluid-and-blood-of-patients-with-alzheimer-s-disease-and-other-types-of-dementia-an-exploratory-study
#15
Sofie Sølvsten Sørensen, Ann-Britt Nygaard, Thomas Christensen
BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNA molecules that function as posttranscriptional regulators of gene expression. Measurements of miRNAs in cerebrospinal fluid (CSF) and blood have just started gaining attention as a novel diagnostic tool for various neurological conditions. The purpose of this exploratory investigation was to analyze the expression of miRNAs in CSF and blood of patients with Alzheimer's disease (AD) and other neurodegenerative disorders in order to identify potential miRNA biomarker candidates able to separate AD from other types of dementia...
2016: Translational Neurodegeneration
https://www.readbyqxmd.com/read/26583473/downregulation-of-mir-27b-is-involved-in-loss-of-type-ii-collagen-by-directly-targeting-matrix-metalloproteinase-13-mmp13-in-human-intervertebral-disc-degeneration
#16
Hao-ran Li, Qing Cui, Zhan-yin Dong, Jian-hua Zhang, Hai-qing Li, Ling Zhao
STUDY DESIGN: A microRNA (miRNA) study. OBJECTIVE: The purpose of this study was to identify intervertebral disc degeneration (IDD)-specific miRNAs, followed by functional validation of results. SUMMARY OF BACKGROUND DATA: IDD is the major contributor to back radicular pain, and the molecular mechanisms underlying this disease are not completely understood. Accumulating evidence suggests that miRNAs play an important role in IDD, but the role of specific miRNAs involved in this disease remains elusive...
February 2016: Spine
https://www.readbyqxmd.com/read/26571175/microrna-221-regulates-hypertrophy-of-ligamentum-flavum-in-lumbar-spinal-stenosis-by-targeting-timp-2
#17
Yun-qiang Xu, Zhen-hui Zhang, Yong-fa Zheng, Shi-qing Feng
STUDY DESIGN: A study of lumbar ligamentum flavum (LF). OBJECTIVE: The aim of this study was to identify LF hypertrophy related microRNAs (miRNAs) expression profile and to investigate the role of miRNAs in the development of LF hypertrophy in lumbar spinal stenosis (LSS). SUMMARY OF BACKGROUND DATA: Although histologic and biologic literature on LF hypertrophy is available, the pathomechanism is still unknown. Accumulating evidence suggests that microRNAs (miRNAs) participate in many physiologic processes, including cell proliferation, differentiation, and fibrosis, but the role of specific miRNAs involved in LF hypertrophy remains elusive...
February 2016: Spine
https://www.readbyqxmd.com/read/26279428/lumbar-disc-degeneration-is-facilitated-by-mir-100-mediated-fgfr3-suppression
#18
Ning Yan, Shunzhi Yu, Hailong Zhang, Tiesheng Hou
BACKGROUND/AIMS: The pathogenesis of lumbar disc degeneration (LDD) involved activation of matrix metalloproteinase 13 (MMP13) by differential expression of fibroblast growth factor receptor 1 (FGFR1) and FGFR3. Nevertheless, the molecular regulation of FGFR1 and FGFR3 in the lumber disc cells remains elusive. METHODS: We examined the FGFR1 and FGFR3 levels and microRNAs (miRNAs) levels in the resected LDD discs, compared to the traumatized, non-LDD discs. We analyzed the binding of miR-100 to the 3'UTR of FGFR3 mRNA and its effects on FGFR3 translation by bioinformatics analysis and by luciferase-reporter assay, respectively...
2015: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/26226413/peripheral-blood-biomarkers-in-multiple-sclerosis
#19
REVIEW
Antonella D'Ambrosio, Simona Pontecorvo, Tania Colasanti, Silvia Zamboni, Ada Francia, Paola Margutti
Multiple sclerosis is the most common autoimmune disorder affecting the central nervous system. The heterogeneity of pathophysiological processes in MS contributes to the highly variable course of the disease and unpredictable response to therapies. The major focus of the research on MS is the identification of biomarkers in biological fluids, such as cerebrospinal fluid or blood, to guide patient management reliably. Because of the difficulties in obtaining spinal fluid samples and the necessity for lumbar puncture to make a diagnosis has reduced, the research of blood-based biomarkers may provide increasingly important tools for clinical practice...
December 2015: Autoimmunity Reviews
https://www.readbyqxmd.com/read/26222740/identification-of-spinal-cord-microrna-and-gene-signatures-in-a-model-of-chronic-stress-induced-visceral-hyperalgesia-in-rat
#20
Sylvie Bradesi, Iordanes Karagiannides, Kyriaki Bakirtzi, Swapna Mahurkar Joshi, Georgios Koukos, Dimitrios Iliopoulos, Charalabos Pothoulakis, Emeran A Mayer
INTRODUCTION: Animal studies have shown that stress could induce epigenetic and transcriptomic alterations essential in determining the balance between adaptive or maladaptive responses to stress. We tested the hypothesis that chronic stress in rats deregulates coding and non-coding gene expression in the spinal cord, which may underline neuroinflammation and nociceptive changes previously observed in this model. METHODS: Male Wistar rats were exposed to daily stress or handled, for 10 days...
2015: PloS One
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