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https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#1
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890889/first-molecular-detection-of-tick-borne-pathogens-in-dogs-from-jiangxi-china
#2
Weiqing Zheng, Mingming Liu, Paul Franck Adjou Moumouni, Xiaoqing Liu, Artemis Efstratiou, Zhanbin Liu, Yangqing Liu, Huiying Tao, Huanping Guo, Guanbo Wang, Yang Gao, Zifen Li, Aaron Edmund Ringo, Charoonluk Jirapattharasate, Haiying Chen, Xuenan Xuan
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp...
November 26, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27884946/analysis-of-the-caenorhabditis-elegans-innate-immune-response-to-coxiella-burnetii
#3
James M Battisti, Lance A Watson, Myo T Naung, Adam M Drobish, Ekaterina Voronina, Michael F Minnick
The nematode Caenorhabditis elegans is well established as a system for characterization and discovery of molecular mechanisms mediating microbe-specific inducible innate immune responses to human pathogens. Coxiella burnetii is an obligate intracellular bacterium that causes a flu-like syndrome in humans (Q fever), as well as abortions in domesticated livestock, worldwide. Initially, when wild type C. elegans (N2 strain) was exposed to mCherry-expressing C. burnetii (CCB) a number of overt pathological manifestations resulted, including intestinal distension, deformed anal region and a decreased lifespan...
November 24, 2016: Innate Immunity
https://www.readbyqxmd.com/read/27876593/intact-interferon-%C3%AE-response-against-coxiella-burnetii-by-peripheral-blood-mononuclear-cells-in-chronic-q-fever
#4
Teske Schoffelen, Julien Textoris, Chantal P Bleeker-Rovers, Amira Ben Amara, Jos W M van der Meer, Mihai G Netea, Jean-Louis Mege, Marcel van Deuren, Esther van de Vosse
OBJECTIVES: Q fever is caused by Coxiella burnetii, an intracellular bacterium that infects phagocytes. The aim of the present study was to investigate whether the C. burnetii-induced IFN-γ response is defective in chronic Q fever patients. METHODS: IFN-γ was measured in supernatants of C. burnetii-stimulated peripheral blood mononuclear cells (PBMCs) of 17 chronic Q fever patients and 17 healthy individuals. To assess IFN-γ responses, expression profiles of IFN-γ-induced genes in C...
November 19, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27869590/detection-and-genotyping-of-coxiella-burnetii-in-pigs-south-korea-2014-2015
#5
Min-Goo Seo, In-Ohk Ouh, Seung-Hun Lee, Dongmi Kwak
We assessed Coxiella burnetii prevalence and genotypes in pigs in South Korea during 2014-2015. Prevalence was low among 1,030 samples tested by ELISA and immunofluorescent assay and 1,124 samples tested by PCR. Despite this finding, possible transmission of C. burnetii from pigs to humans cannot be excluded.
December 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27856520/from-q-fever-to-coxiella-burnetii-infection-a-paradigm-change
#6
REVIEW
Carole Eldin, Cléa Mélenotte, Oleg Mediannikov, Eric Ghigo, Matthieu Million, Sophie Edouard, Jean-Louis Mege, Max Maurin, Didier Raoult
Coxiella burnetii is the agent of Q fever, or "query fever," a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans...
January 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/27845064/the-burden-of-coxiella-burnetii-among-aborted-dairy-animals-in-egypt-and-its-public-health-implications
#7
Khaled A Abdel-Moein, Dalia A Hamza
Q fever is a zoonotic disease of mounting public health implications. Dairy animals are major reservoir for such disease whereas abortion is the main clinical outcome. The current study was conducted to investigate the burden of C. burnetii abortions among dairy animals in Egypt to provide more knowledge for better control of such disease. For this purpose, placental cotyledons and vaginal discharges from 108 aborted dairy animals (27 sheep, 29 goats, 26 cattle, 26 buffaloes) were examined for the presence of C...
November 11, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27836035/detection-and-risk-factors-of-coxiella-burnetii-infection-in-dairy-cattle-based-on-bulk-tank-milk-samples-in-center-of-iran
#8
Zary Nokhodian, Awat Feizi, Abdolreza Moradi, Majid Yaran, Shervin Ghaffari Hoseini, Behrooz Ataei, Mohammad Hosseini
Infection with Coxiella burnetii has a worldwide distribution in ruminants and is associated with abortions and reproductive problems in this group of animals. This study aimed to determine the prevalence of C. burnetii DNA in bulk tank milk (BTM) of dairy cattle herds and to identify the risk factors of infection. This cross-sectional study was conducted in spring 2015. A total of 163 BTM samples from 74 commercial and 89 traditional dairy cattle herds in Isfahan, Iran was tested for the IS1111 transposon of C...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27821696/molecular-evidence-of-malaria-and-zoonotic-diseases-among-rapid-diagnostic-test-negative-febrile-patients-in-low-transmission-season-mali
#9
Mahamoudou Touré, Pelle T Petersen, Sidy N'd Bathily, Daouda Sanogo, Christian W Wang, Karin L Schiøler, Flemming Konradsen, Seydou Doumbia, Michael Alifrangis
From November to December 2012 in Sélingué-Mali, blood samples from 88 febrile patients who tested negative by malaria Paracheck (®) rapid diagnostic tests (RDTs) were used to assess the presence of sub-RDT Plasmodium falciparum as well as Borrelia, Coxiella burnetii, and Babesia applying molecular tools. Plasmodium sp. was present among 57 (60.2%) of the 88 malaria RDT-negative patients, whereas the prevalence of Borrelia, C. burnetii, and Babesia were 3.4% (N = 3), 1.1% (N = 1), and 0.0%, respectively...
November 7, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27806553/a-survey-of-acute-q-fever-among-patients-with-brucellosis-like-and-atypical-pneumonia-symptoms-who-are-referred-to-qaemshahr-razi-hospital-in-northern-iran-2014-2015
#10
Roya Ghasemian, Ehsan Mostafavi, Saber Esmaeili, Narges Najafi, Sara Arabsheybani
INTRODUCTION: Coxiella burnetii is the etiologic agent of the zoonotic disease of Q fever which causes lots of morbidities and mortalities every year. The main route of human infection is inhalation of contaminated aerosols; however, consumption of contaminated dairy products is the second cause. Mazandaran province is one of the main livestock centers of the country and consumption of raw dairy in the region is quite common. There isn't any data about Q fever incidence in this region...
August 26, 2016: Global Journal of Health Science
https://www.readbyqxmd.com/read/27792764/molecular-detection-and-genotyping-of-coxiella-like-endosymbionts-in-ticks-that-infest-horses-in-south-korea
#11
Min-Goo Seo, Seung-Hun Lee, In-Ohk Ouh, Gwang Hyeop Lee, Youn-Kyoung Goo, Seungjoon Kim, Oh-Deog Kwon, Dongmi Kwak
Members of the genus Coxiella can be transmitted from ticks to humans during contact with animals; Coxiella may thus spread from the infected horses or ticks to humans. In this study, the presence of Coxiella burnetii and Coxiella-like endosymbionts (CLE) in ticks found on infested horses was determined using PCR and genotyping. A total of 213 ticks were randomly collected from 51 horses (4-5 ticks per horse) raised on Jeju Island, Korea, between 2009 and 2013. All ticks were morphologically identified as adult Haemaphysalis longicornis, a predominant tick species widespread in Korea...
2016: PloS One
https://www.readbyqxmd.com/read/27776577/wide-exposure-to-coxiella-burnetii-in-ruminant-and-feline-species-living-in-a-natural-environment-zoonoses-in-a-human-livestock-wildlife-interface
#12
M G Candela, A Caballol, P M Atance
Assessment of the role of wild and domestic hosts as potential reservoirs of misdiagnosed zoonoses, such as Q fever by Coxiella burnetii, is an important public health issue today both for wildlife conservation and management of disease in human-livestock-wildlife interface. This study used ELISA, an indirect antibody, to research (2003-2013) C. burnetii infection in seven free-living wild and domestic ruminant species and in European wildcats (Felis silvestris). The animals studied were 0 European wildcats, 21 Spanish ibex (Capra pyrenaica), 314 red deer (Cervus elaphus), 556 fallow deer (Dama dama), 211 European mouflon (Ovis aries musimon), eight roe deer (Capreolus capreolus), 407 bovines (Bos taurus) and 3739 sheep (Ovis aries)...
October 25, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27771116/coxiella-burnetii-detected-in-three-species-of-endangered-north-african-gazelles-that-recently-aborted
#13
Elena García, Gerardo Espeso, Rocío Fernández, Ángel Gómez-Martín, José María Rodríguez-Linde, Christian De la Fe
Coxiella (C.) burnetii is the etiological agent of the zoonotic disease known a Q fever. This agent can infect multiple hosts although its pathogenic potential in wild ruminants has been poorly studied. The polymerase chain reaction and the serological test detected the presence of C. burnetii in a population of North African gazelles (n = 355), comprising dorcas gazelle (Gazella dorcas neglecta), dama gazelle (Nanger dama mhorr) and Cuvier's gazelle (Gazella cuvieri) which, some of them, they recently aborted...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27768623/spatial-prediction-of-coxiella-burnetii-outbreak-exposure-via-notified-case-counts-in-a-dose-response-model
#14
Russell J Brooke, Mirjam E E Kretzschmar, Volker Hackert, Christian J P A Hoebe, Peter F M Teunis, Lance A Waller
We develop a novel approach to study an outbreak of Q fever in 2009 in the Netherlands by combining a human dose-response model with geostatistics prediction to relate probability of infection and associated probability of illness to an effective dose of Coxiella burnetii. The spatial distribution of the 220 notified cases in the at-risk population are translated into a smooth spatial field of dose. Based on these symptomatic cases, the dose-response model predicts a median of 611 asymptomatic infections (95% range: 410, 1,084) for the 220 reported symptomatic cases in the at-risk population; 2...
January 2017: Epidemiology
https://www.readbyqxmd.com/read/27763821/coxiella-detection-in-ticks-from-wildlife-and-livestock-in-malaysia
#15
Jing-Jing Khoo, Fang-Shiang Lim, Fezshin Chen, Wai-Hong Phoon, Chee-Sieng Khor, Brian L Pike, Li-Yen Chang, Sazaly AbuBakar
Recent studies have shown that ticks harbor Coxiella-like bacteria, which are potentially tick-specific endosymbionts. We recently described the detection of Coxiella-like bacteria and possibly Coxiella burnetii in ticks found from rural areas in Malaysia. In the present study, we collected ticks, including Haemaphysalis bispinosa, Haemaphysalis hystricis, Dermacentor compactus, Dermacentor steini, and Amblyomma sp. from wildlife and domesticated goats from four different locations in Malaysia. Coxiella 16s rRNA genomic sequences were detected by PCR in 89% of ticks tested...
October 20, 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27762219/coxiella-burnetii-chronic-pericarditis-a-case-report
#16
I Ivić, I Božić, D Ledina
Coxiella burnetii is capable of causing a variety of acute and chronic infections. We present a case of pericarditis with serologically confirmed chronic C. burnetii infection. This case report emphasises the justification of serological testing for chronic C. burnetii infection in patients with prolonged or recurrent pericarditis, particularly in countries endemic for C. burnetii infection.
October 2016: Netherlands Journal of Medicine
https://www.readbyqxmd.com/read/27744637/bacteriological-analysis-of-saliva-from-partially-or-fully-engorged-female-adult-rhipicephalus-microplus-by-next-generation-sequencing
#17
Liangliang Xiang, Błażej Poźniak, Tian-Yin Cheng
Tick-borne diseases are a major epidemiological problem worldwide. The aim of this study was to investigate the bacterial composition of saliva obtained from engorged adult Rhipicephalus microplus females. Saliva samples collected from partially or fully engorged adult female ticks were analysed using an ultra-high-throughput Illumina HiSeq 2500 sequencing system. To elucidate the possible routes of bacterial transmission, the bacterial flora from whole ticks were also investigated. Proteobacteria, Firmicutes, and Actinobacteria were the predominant phyla in all samples, and Acinetobacter, Rickettsia, Escherichia and Coxiella were the major genera...
October 15, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27736781/coxiella-burnetii-avirulent-nine-mile-phase-ii-induces-caspase-1-dependent-pyroptosis-in-murine-peritoneal-b1a-b-cells
#18
Laura Schoenlaub, Rama Cherla, Yan Zhang, Guoquan Zhang
Our recent study demonstrated that virulent Coxiella burnetii Nine Mile phase I (NMI) is capable of infecting and replicating within peritoneal B1a cells and that B1a cells play an important role in host defense against C. burnetii infection in mice. However, it remains unknown if avirulent Nine Mile phase II (NMII) can infect and replicate in B1a cells and whether NMI and NMII can differentially interact with B1a cells. In this study, we examined if NMI and NMII can differentially modulate host cell apoptotic signaling in B1a cells...
October 10, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27726597/analysis-of-coxiela-burnetti-dihydrofolate-reductase-via-in-silico-docking-with-inhibitors-and-molecular-dynamics-simulation
#19
Felipe Rodrigues de Souza, Ana Paula Guimarães, Teobaldo Cuya, Matheus Puggina de Freitas, Arlan da Silva Gonçalves, Pat Forgione, Tanos Celmar Costa França
Coxiella burnetii is a gram-negative bacterium able to infect several eukaryotic cells, mainly monocytes and macrophages. It is found widely in nature with ticks, birds, and mammals as major hosts. C. burnetii is also the biological warfare agent that causes Q fever, a disease that has no vaccine or proven chemotherapy available. Considering the current geopolitical context, this fact reinforces the need for discovering new treatments and molecular targets for drug design against C. burnetii. Among the main molecular targets against bacterial diseases reported, the enzyme dihydrofolate reductase (DHFR) has been investigated for several infectious diseases...
October 11, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27716804/the-sero-epidemiology-of-coxiella-burnetii-in-humans-and-cattle-western-kenya-evidence-from-a-cross-sectional-study
#20
Nicola A Wardrop, Lian F Thomas, Elizabeth A J Cook, William A de Glanville, Peter M Atkinson, Claire N Wamae, Eric M Fèvre
Evidence suggests that the intracellular bacterial pathogen Coxiella burnetii (which causes Q fever) is widespread, with a near global distribution. While there has been increasing attention to Q fever epidemiology in high-income settings, a recent systematic review highlighted significant gaps in our understanding of the prevalence, spatial distribution and risk factors for Q fever infection across Africa. This research aimed to provide a One Health assessment of Q fever epidemiology in parts of Western and Nyanza Provinces, Western Kenya, in cattle and humans...
October 2016: PLoS Neglected Tropical Diseases
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