keyword
MENU ▼
Read by QxMD icon Read
search

humanized mouse and cancer

keyword
https://www.readbyqxmd.com/read/29684274/preclinical-development-of-novel-psma-targeting-radioligands-modulation-of-albumin-binding-properties-to-improve-prostate-cancer-therapy
#1
Christoph A Umbricht, Martina Benesova, Roger Schibli, Cristina Müller
The treatment of metastatic castration-resistant prostate cancer (mCRPC) remains challenging with current treatment options. The development of more effective therapies is, therefore, urgently needed. Targeted radionuclide therapy with prostate-specific membrane antigen (PSMA)-targeting ligands has revealed promising clinical results. In an effort to optimize this concept, it was the aim of this study to design and investigate PSMA ligands comprising different types of albumin binders. PSMA-ALB-53 and PSMA-ALB-56 were designed by combining the glutamate-urea PSMA-binding entity, a DOTA chelator and an albumin binder based on the 4-(p-iodophenyl)-moiety or p-(tolyl)-moiety...
April 23, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29683462/polysome-profiling-in-leishmania-human-cells-and-mouse-testis
#2
Zemfira N Karamysheva, Elena B Tikhonova, Petar N Grozdanov, James C Huffman, Kristen R Baca, Alexander Karamyshev, R Brian Denison, Clinton C MacDonald, Kai Zhang, Andrey L Karamyshev
Proper protein expression at the right time and in the right amounts is the basis of normal cell function and survival in a fast-changing environment. For a long time, the gene expression studies were dominated by research on the transcriptional level. However, the steady-state levels of mRNAs do not correlate well with protein production, and the translatability of mRNAs varies greatly depending on the conditions. In some organisms, like the parasite Leishmania, the protein expression is regulated mostly at the translational level...
April 8, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29683343/allicin-inhibits-mouse-colorectal-tumorigenesis-through-suppressing-the-activation-of-stat3-signaling-pathway
#3
Xiang Li, Jiahui Ni, Yixin Tang, Xu Wang, Huanhuan Tang, Haidong Li, Sulin Zhang, Xiaoyan Shen
Allicin is the major biologically active compounds of freshly crushed garlic. It has been reported to inhibit the proliferation and promote the apoptosis of multiple colorectal cancer cells. However, the anti-colorectal cancer effect of Allicin has not been verified by in vivo studies. In the present study, we investigated the effect of Allicin on azoxymethane/dextran sodium sulfate (AOM/DSS) colorectal cancer mouse model and explore the underlying possible mechanism. Our result showed that Allicin could inhibit colonic tumorigenesis of AOM/DSS mice in vivo...
April 23, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29683229/stromal-immunoglobulin-%C3%AE%C2%BAc-expression-is-associated-with-initiation-of-breast-cancer-in-ta2-mice-and-human-breast-cancer
#4
Shiwu Zhang, Fei Fei, Hua Wang, Yanjun Gu, Chunyuan Li, Xinlu Wang, Yongjie Zhao, Yuwei Li
The initiation of spontaneous breast cancer (SBC) in Tientsin Albino 2 (TA2) mice is related to mouse mammary tumor virus (MMTV) infection, and MMTV amplification is hormonally regulated. To explore the insertion site of MMTVLTR in TA2 mouse genome, reverse PCR and nested PCR were used to amplify the unknown sequence on both sides of the MMTV-LTRSAG gene in SBC and normal breast tissue of TA2 mice. Furthermore, the clinicopathological significance of the insertion site was evaluated in 43 samples of normal breast tissue, 46 samples of breast cystic hyperplasia, 54 samples of ductal carcinoma in situ, 142 samples of primary breast cancer, and 47 samples of lymph node metastatic breast cancer by RNA in situ hybridization...
April 23, 2018: Cancer Science
https://www.readbyqxmd.com/read/29682172/momelotinib-decreased-cancer-stem-cell-associated-tumor-burden-and-prolonged-disease-free-remission-period-in-a-mouse-model-of-human-ovarian-cancer
#5
Emily Chan, Rodney Luwor, Christopher Burns, George Kannourakis, Jock K Findlay, Nuzhat Ahmed
Despite a good initial response to front-line chemotherapy, majority of the ovarian cancer patients relapse with consecutive phases of recurrences; and nearly 60% die within 5 years due to the development of a chemoresistant disease. This study investigated whether inhibition of the Janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) pathway by momelotinib is sufficient in suppressing tumor burden and prolonging the disease-free survival period in a mouse model of ovarian cancer...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29679033/innovatively-therapeutic-strategy-on-lung-cancer-by-daily-drinking-antioxidative-plasmon-induced-activated-water
#6
Chien-Kai Wang, Hsiao-Chien Chen, Sheng-Uei Fang, Chia-Wen Ho, Cheng-Jeng Tai, Chih-Ping Yang, Yu-Chuan Liu
Many human diseases are inflammation-related, such as cancer and those associated with aging. Previous studies demonstrated that plasmon-induced activated (PIA) water with electron-doping character, created from hot electron transfer via decay of excited Au nanoparticles (NPs) under resonant illumination, owns reduced hydrogen-bonded networks and physchemically antioxidative properties. In this study, it is demonstrated PIA water dramatically induced a major antioxidative Nrf2 gene in human gingival fibroblasts which further confirms its cellular antioxidative and anti-inflammatory properties...
April 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29678874/mitochondrial-morphological-and-functional-reprogramming-following-cd137-4-1bb-co-stimulation
#7
Alvaro Teijeira, Sara Labiano, Saray Garasa, Inaki Etxeberria, Eva Santamaria, Ana Rouzaut, Michel Enamorado, Arantza Azpilikueta, Susana Inoges, Elixabet Bolanos-Mateo, Maria Angela Aznar, Alfonso R Sanchez-Paulete, David Sancho, Ignacio Melero
T and NK lymphocytes express CD137 (4-1BB), a costimulatory receptor of the TNFR family whose function is exploitable for cancer immunotherapy. Mitochondria regulate the function and survival of T lymphocytes. Herein, we show that CD137 costimulation provided by agonist mAb and CD137L (4-1BBL) induced mitochondria enlargement that resulted in enhanced mitochondrial mass and transmembrane potential in human and mouse CD8+ T cells. Such mitochondrial changes increased T-cell respiratory capacities and were critically dependent on mitochondrial fusion protein OPA-1 expression...
April 20, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29677116/human-cancer-and-platelet-interaction-a-potential-therapeutic-target
#8
REVIEW
Shike Wang, Zhenyu Li, Ren Xu
Cancer patients experience a four-fold increase in thrombosis risk, indicating that cancer development and progression are associated with platelet activation. Xenograft experiments and transgenic mouse models further demonstrate that platelet activation and platelet-cancer cell interaction are crucial for cancer metastasis. Direct or indirect interaction of platelets induces cancer cell plasticity and enhances survival and extravasation of circulating cancer cells during dissemination. In vivo and in vitro experiments also demonstrate that cancer cells induce platelet aggregation, suggesting that platelet-cancer interaction is bidirectional...
April 20, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29675041/antitumor-activity-of-intratumoral-ethanol-injection-in-an-orthotopic-pancreatic-cancer-cell-mouse-xenograft-model
#9
Wen-Ying Zhang, Zhen-Dong Jin, Feng Liu, Hai-Hua Yuan, Bin Jiang
Purpose: Pancreatic cancer is a lethal disease and usually is diagnosed at advanced stages of disease. This study assessed the effects of intratumoral ethanol injection using an endoscopic ultrasound (EUS) probe on the control of pancreatic cancer in a mouse orthotopic xenograft model. Materials and Methods: The subcutaneous and orthotopic human pancreatic cancer cell mouse xenograft models were established. Different concentrations of ethanol (0-95%) were injected into subcutaneous xenograft tumors...
2018: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/29674421/anti-misrii-radiolabeled-antibodies-new-tools-for-a-theranostic-approach-in-ovarian-cancer
#10
Emmanuel Deshayes, Riad Ladjohounlou, Pierre Le Fur, Alexandre Pichard, Catherine Lozza, Vincent Boudousq, Samuel Sevestre, Marta Jarlier, Roxana Kashani, Joanna Koch, Jane Sosabowski, Julie Foster, Nicolas Chouin, Frank Bruchertseifer, Alfred Morgenstern, Pierre-Olivier Kotzki, Isabelle Navarro-Teulon, Jean-Pierre Pouget
We have developed the 16F12 mouse monoclonal antibody (mAb) that targets the MISRII receptor expressed by ovarian tumors. Here, we assessed in preclinical models the possibility to use radiolabeled 16F12 in a theranostic approach for small-volume ovarian peritoneal carcinomatosis, for instance after cytoreductive surgery. Methods: DOTA-, DTPA- or DFO-conjugated 16F12 mAb was radiolabeled with beta (177 Lu) or alpha (213 Bi) particle emitters for therapeutic use, and with 89 Zr for positron emission tomography (PET) imaging...
April 19, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29674294/discovery-of-new-benzensulfonamide-derivatives-as-tripedal-stat3-inhibitors
#11
Jianpeng Guo, Wenying Yu, Guiping Cai, Wenda Zhang, Shanshan Li, Jiawen Zhu, Dongmei Song, Lingyi Kong
Persistent activated STAT3 has a striking correlation with cancer development and inhibition of STAT3 signaling pathway is a novel therapeutic way for human cancers. Among STAT family, STAT1 and STAT3 play opposite roles in tumorigenesis. However, the discovery of selective STAT3 inhibitors is still challenging to date. In this study, a series of small-molecular (MW < 500) benzensulfanilamide derivatives were designed to selectively suppress STAT3 activation for anti-cancer treatment. The most potent compound 11 inhibited both overexpressed and IL-6 induced STAT3 phosphorylation, whereas 11 displayed little effect on the phosphorylation of other STAT isoforms STAT1, STAT5, demonstrating 11 was a selective STAT3 inhibitor...
April 4, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29674274/a-novel-synthetic-curcumin-derivative-mhmm-41-induces-ros-mediated-apoptosis-and-migration-blocking-of-human-lung-cancer-cells-a549
#12
Guang-Zhou Zhou, A-Fang Li, Yan-He Sun, Gang-Chun Sun
Many curcumin derivatives were produced and characterized to improve the physiochemical instability and low solubility of curcumin. Here, MHMM-41 (a novel curcumin derivative) was used to treat non-small lung cancer cells of human (known as A549) and to identify its anti-proliferative activities. Our results suggested that MHMM-41 display no significant cytotoxicity toward normal human lung fibroblast 2BS cells and mouse embryonal fibroblast 3T3 cells. It also had better anti-proliferative activity than curcumin in A549 cells...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29672884/oxidatively-modified-pdia3-promotes-dkc1-mediated-malignancy-and-survival-of-hepatocellular-carcinoma-cells
#13
Eunkyong Ko, Jong-Seo Kim, Soomi Ju, Hyun-Wook Seo, Yeonji Chang, Jung-Ah Kang, Sung-Gyoo Park, Guhung Jung
DKC1 is a conserved gene encoding the RNA-binding protein, dyskerin pseudouridine synthase 1, which is an essential component of the telomerase holoenzyme. DKC1 upregulation is frequently observed in many different human cancers including hepatocellular carcinoma (HCC); however, its regulatory mechanisms still remain unclear. Thus, we investigated the regulatory mechanism of DKC1 in HCC progression. We found that protein-disulfide isomerase-associated 3 (PDIA3) interacted with the DKC1 regulatory DNA in HCC cells, but not in HCC cells with elevated reactive oxygen species (ROS) levels, using liquid chromatography tandem-mass spectrometry analysis after isolating the DKC1 regulatory region binding proteins...
April 19, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29671273/intravital-imaging-of-human-melanoma-cells-in-the-mouse-ear-skin-by-two-photon-excitation-microscopy
#14
Nathan Y Bentolila, Raymond L Barnhill, Claire Lugassy, Laurent A Bentolila
Noninvasive imaging of reporter gene expression by two-photon excitation (2PE) laser scanning microscopy is uniquely suited to perform dynamic and multidimensional imaging down to single-cell detection sensitivity in vivo in deep tissues. Here we used 2PE microscopy to visualize green fluorescent protein (GFP) as a reporter gene in human melanoma cells implanted into the dermis of the mouse ear skin. We first provide a step-by-step methodology to set up a 2PE imaging model of the mouse ear's skin and then apply it for the observation of the primary tumor and its associated vasculature in vivo...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29670684/uva-irradiation-enhances-brusatol-mediated-inhibition-of-melanoma-growth-by-downregulation-of-the-nrf2-mediated-antioxidant-response
#15
Mei Wang, Guangwei Shi, Chunxiang Bian, Muhammad Farrukh Nisar, Yingying Guo, Yan Wu, Wei Li, Xiao Huang, Xuemei Jiang, Jörg W Bartsch, Ping Ji, Julia Li Zhong
Brusatol (BR) is a potent inhibitor of Nrf2, a transcription factor that is highly expressed in cancer tissues and confers chemoresistance. UVA-generated reactive oxygen species (ROS) can damage both normal and cancer cells and may be of potential use in phototherapy. In order to provide an alternative method to treat the aggressive melanoma, we sought to investigate whether low-dose UVA with BR is more effective in eliminating melanoma cells than the respective single treatments. We found that BR combined with UVA led to inhibition of A375 melanoma cell proliferation by cell cycle arrest in the G1 phase and triggers cell apoptosis...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29670173/overexpression-of-her2-in-the-pancreas-promotes-development-of-intraductal-papillary-mucinous-neoplasms-in-mice
#16
Wataru Shibata, Hiroto Kinoshita, Yohko Hikiba, Takeshi Sato, Yasuaki Ishii, Soichiro Sue, Makoto Sugimori, Nobumi Suzuki, Kosuke Sakitani, Hideaki Ijichi, Ryutaro Mori, Itaru Endo, Shin Maeda
Pancreatic ductal adenocarcinoma (PDA) has a 5-year survival rate of less than 5% and is the sixth leading cause of cancer death. Although KRAS mutations are one of the major driver mutations in PDA, KRAS mutation alone is not sufficient to induce invasive pancreatic cancer in mice model. HER2, also known as ERBB2, is a receptor tyrosine kinase, and overexpression of HER2 is associated with poor clinical outcomes in pancreatic cancer. However, no report has shown whether HER2 and its downstream signaling contributes to the pancreatic cancer development...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29670110/tumor-associated-macrophages-promote-progression-and-the-warburg-effect-via-ccl18-nf-kb-vcam-1-pathway-in-pancreatic-ductal-adenocarcinoma
#17
Huilin Ye, Quanbo Zhou, Shangyou Zheng, Guolin Li, Qing Lin, Lusheng Wei, Zhiqiang Fu, Bin Zhang, Yimin Liu, Zhihua Li, Rufu Chen
Tumor-associated macrophages (TAMs) are frequently found near pancreatic cancer cells, but it is uncertain whether they are involved in pancreatic cancer progression and the Warburg effect. Here, we show that CCL18 secreted by TAMs facilitates malignant progression and induced a glycolytic phenotype in pancreatic cancer, partially owing to paracrine induction of VCAM-1 in pancreatic cancer cells. Reciprocally, VCAM-1-induced lactate production from pancreatic cancer cells with enhanced aerobic glycolysis activates macrophages to a TAM-like phenotype, forming a positive feedback loop...
April 18, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29670099/nuclear-receptor-nr2f6-inhibition-potentiates-responses-to-pd-l1-pd-1-cancer-immune-checkpoint-blockade
#18
Victoria Klepsch, Natascha Hermann-Kleiter, Patricia Do-Dinh, Bojana Jakic, Anne Offermann, Mirjana Efremova, Sieghart Sopper, Dietmar Rieder, Anne Krogsdam, Gabriele Gamerith, Sven Perner, Alexandar Tzankov, Zlatko Trajanoski, Dominik Wolf, Gottfried Baier
Analyzing mouse tumor models in vivo, human T cells ex vivo, and human lung cancer samples, we provide direct evidence that NR2F6 acts as an immune checkpoint. Genetic ablation of Nr2f6, particularly in combination with established cancer immune checkpoint blockade, efficiently delays tumor progression and improves survival in experimental mouse models. The target genes deregulated in intratumoral T lymphocytes upon genetic ablation of Nr2f6 alone or together with PD-L1 blockade reveal multiple advantageous transcriptional alterations...
April 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29669940/generation-and-testing-of-clinical-grade-exosomes-for-pancreatic-cancer
#19
Mayela Mendt, Sushrut Kamerkar, Hikaru Sugimoto, Kathleen M McAndrews, Chia-Chin Wu, Mihai Gagea, Sujuan Yang, Elena V Rodriges Blanko, Qian Peng, Xiaoyan Ma, Joseph R Marszalek, Anirban Maitra, Cassian Yee, Katayoun Rezvani, Elizabeth Shpall, Valerie S LeBleu, Raghu Kalluri
Exosomes are extracellular vesicles produced by all cells with a remarkable ability to efficiently transfer genetic material, including exogenously loaded siRNA, to cancer cells. Here, we report on a bioreactor-based, large-scale production of clinical-grade exosomes employing good manufacturing practice (GMP) standards. A standard operating procedure was established to generate engineered exosomes with the ability to target oncogenic Kras (iExosomes). The clinical-grade GMP iExosomes were tested in multiple in vitro and in vivo studies to confirm suppression of oncogenic Kras and an increase in the survival of several mouse models with pancreatic cancer...
April 19, 2018: JCI Insight
https://www.readbyqxmd.com/read/29669936/engineered-t-cells-targeting-e7-mediate-regression-of-human-papillomavirus-cancers-in-a-murine-model
#20
Benjamin Y Jin, Tracy E Campbell, Lindsey M Draper, Sanja Stevanović, Bianca Weissbrich, Zhiya Yu, Nicholas P Restifo, Steven A Rosenberg, Cornelia L Trimble, Christian S Hinrichs
T cell receptor (TCR) T cell therapy is a promising cancer treatment modality. However, its successful development for epithelial cancers may depend on the identification of high-avidity TCRs directed against tumor-restricted target antigens. The human papillomavirus (HPV) E7 antigen is an attractive therapeutic target that is constitutively expressed by HPV+ cancers but not by healthy tissues. It is unknown if genetically engineered TCR T cells that target E7 can mediate regression of HPV+ cancers. We identified an HPV-16 E7-specific, HLA-A*02:01-restricted TCR from a uterine cervix biopsy from a woman with cervical intraepithelial neoplasia...
April 19, 2018: JCI Insight
keyword
keyword
106187
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"