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https://www.readbyqxmd.com/read/28550642/post-heroin-dose-tissue-distribution-of-6-monoacetylmorphine-6-mam-with-maldi-imaging
#1
Belin G Teklezgi, Annapurna Pamreddy, Sooraj Baijnath, Nirmala D Gopal, Tricia Naicker, Hendrik G Kruger, Thavendran Govender
Heroin is an illicit opioid drug which is commonly abused and leads to dependence and addiction. Heroin is considered a pro-drug and is rapidly converted to its major active metabolite 6-monoacetylmorphine (6-MAM) which mediates euphoria and reward through the stimulation of opioid receptors in the brain. The aim of this study was to investigate the distribution and localization of 6-MAM in the healthy Sprague Dawley rat brain following intraperitoneal (i.p) administration of heroin (10 mg/kg), using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), in combination with quantification via liquid chromatography mass spectrometry (LC-MS/MS)...
May 26, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28549769/variations-in-the-cerebrospinal-fluid-proteome-following-traumatic-brain-injury-and-subarachnoid-hemorrhage
#2
David E Connor, Ganta V Chaitanya, Prashant Chittiboina, Paul McCarthy, L Keith Scott, Lisa Schrott, Alireza Minagar, Anil Nanda, J Steven Alexander
BACKGROUND: Proteomic analysis of cerebrospinal fluid (CSF) has shown great promise in identifying potential markers of injury in neurodegenerative diseases [1-13]. Here we compared CSF proteomes in healthy individuals, with patients diagnosed with traumatic brain injury (TBI) and subarachnoid hemorrhage (SAH) in order to characterize molecular biomarkers which might identify these different clinical states and describe different molecular mechanisms active in each disease state. METHODS: Patients presenting to the Neurosurgery service at the Louisiana State University Hospital-Shreveport with an admitting diagnosis of TBI or SAH were prospectively enrolled...
May 13, 2017: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/28546834/cyclodextrins-tethered-with-oligolactides-green-synthesis-and-structural-assessment
#3
Cristian Peptu, Mihaela Balan-Porcarasu, Alena Šišková, Ľudovít Škultéty, Jaroslav Mosnáček
Biodegradable oligolactide derivatives based on α-, β- and γ-cyclodextrins (CDs) were synthesized by a green procedure in which CDs play the role of both the initiator and the catalyst. The synthetic procedure in which CDs and L-lactide (L-LA) are reacting in bulk at relatively high temperature of 110 °C was investigated considering the structural composition of the products. The obtained products were thoroughly characterized via mass spectrometry methods with soft ionization like matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI)...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28545528/comparison-of-biomarker-based-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-and-conventional-methods-in-the-identification-of-clinically-relevant-bacteria-and-yeast
#4
Ali Kassim, Valentin Pflüger, Zul Premji, Claudia Daubenberger, Gunturu Revathi
BACKGROUND: MALDI-TOF MS is an analytical method that has recently become integral in the identification of microorganisms in clinical laboratories. It relies on databases that majorly employ pattern recognition or fingerprinting. Biomarker based databases have also been developed and there is optimism that these may be superior to pattern recognition based databases. This study compared the performance of ribosomal biomarker based MALDI-TOF MS and conventional methods in the identification of selected bacteria and yeast...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28542393/head-to-head-comparison-of-microflex-lt-and-vitek-ms-systems-for-routine-identification-of-microorganisms-by-maldi-tof-mass-spectrometry-in-chile
#5
Lorena Porte, Patricia García, Stephanie Braun, María Teresa Ulloa, Mónica Lafourcade, Alisson Montaña, Carolina Miranda, Gerardo Acosta-Jamett, Thomas Weitzel
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a new and revolutionary identification method for microorganisms and has recently been introduced into clinical microbiology in many industrialized countries in Europe and North America. OBJECTIVES: Our study aimed to compare the performance and practicality of two commercial MALDI-TOF MS platforms in a head-to head manner at a routine laboratory in Chile...
2017: PloS One
https://www.readbyqxmd.com/read/28540462/development-of-a-hydrophilic-interaction-liquid-chromatography-coupled-with-matrix-assisted-laser-desorption-ionization-mass-spectrometric-imaging-platform-for-n-glycan-relative-quantitation-using-stable-isotope-labeled-hydrazide-reagents
#6
Zhengwei Chen, Xuefei Zhong, Cai Tie, Bingming Chen, Xinxiang Zhang, Lingjun Li
In this work, the capability of newly developed hydrophilic interaction liquid chromatography (HILIC) coupled with matrix-assisted laser desorption/ionization-mass spectrometric imaging (MALDI-MSI) platform for quantitative analysis of N-glycans has been demonstrated. As a proof-of-principle experiment, heavy and light stable-isotope labeled hydrazide reagents labeled maltodextrin ladder were used to demonstrate the feasibility of the HILIC-MALDI-MSI platform for reliable quantitative analysis of N-glycans...
May 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28539340/evaluating-and-improving-vitek-ms%C3%A2-for-identification-of-clinically-relevant-species-of-trichosporon-and-the-close-related-genera-cutaneotrichosporon-and-apiotrichum
#7
João N de Almeida, Viviane M Favero Gimenes, Elaine C Francisco, Lumena P Machado Siqueira, Renato K Gonçalves de Almeida, Juliette Guitard, Christophe Hennequin, Arnaldo Lopes Colombo, Gil Benard, Flavia Rossi
Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, being fast and accurate species identification relevant for hasting the best-targeted therapy. Recently, Trichosporon taxonomy has been re-assessed and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon and Apiotrichum Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism's identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance...
May 24, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28538661/mass-spectrometry-based-profiling-and-imaging-of-various-ginsenosides-from-panax-ginseng-roots-at-different-ages
#8
Jae Won Lee, Seung-Heon Ji, Young-Seob Lee, Doo Jin Choi, Bo-Ram Choi, Geum-Soog Kim, Nam-In Baek, Dae Young Lee
(1) Background: Panax ginseng root is one of the most important herbal products, and the profiling of ginsenosides is critical for the quality control of ginseng roots at different ages in the herbal markets. Furthermore, interest in assessing the contents as well as the localization of biological compounds has been growing. The objective of this study is to carry out the mass spectrometry (MS)-based profiling and imaging of ginsenosides to assess ginseng roots at different ages; (2) Methods: Optimal ultra performance liquid chromatography coupled to quadrupole time of flight/MS (UPLC-QTOF/MS) was used to profile various ginsenosides from P...
May 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28536476/a-chemometrics-driven-strategy-for-the-bioactivity-evaluation-of-complex-multicomponent-systems-and-the-effective-selection-of-bioactivity-predictive-chemical-combinations
#9
Yoshinori Fujimura, Chihiro Kawano, Ayaka Maeda-Murayama, Asako Nakamura, Akiko Koike-Miki, Daichi Yukihira, Eisuke Hayakawa, Takanori Ishii, Hirofumi Tachibana, Hiroyuki Wariishi, Daisuke Miura
Although understanding their chemical composition is vital for accurately predicting the bioactivity of multicomponent drugs, nutraceuticals, and foods, no analytical approach exists to easily predict the bioactivity of multicomponent systems from complex behaviors of multiple coexisting factors. We herein represent a metabolic profiling (MP) strategy for evaluating bioactivity in systems containing various small molecules. Composition profiles of diverse bioactive herbal samples from 21 green tea extract (GTE) panels were obtained by a high-throughput, non-targeted analytical procedure...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#10
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28534157/infrared-laser-ablation-with-vacuum-capture-for-fingermark-sampling
#11
Fabrizio Donnarumma, Eden E Camp, Fan Cao, Kermit K Murray
Infrared laser ablation coupled to vacuum capture was employed to collect material from fingermarks deposited on surfaces of different porosity and roughness. Laser ablation at 3 μm was performed in reflection mode with subsequent capture of the ejecta with a filter connected to vacuum. Ablation and capture of standards from fingermarks was demonstrated on glass, plastic, aluminum, and cardboard surfaces. Using matrix assisted laser desorption ionization (MALDI), it was possible to detect caffeine after spiking with amounts as low as 1 ng...
May 22, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28533144/discovery-of-two-p-superfamily-conotoxins-lt9a-and-lt9b-with-different-modifications-on-voltage-sensitive-sodium-channels
#12
Lei Wang, Junliang Liu, Zhenghua Ren, Yu Chen, Anlong Xu
In this work, two P-superfamily conotoxins, lt9a and lt9b, were purified and characterized from the crude venom of Conus litteratus. The amino acid sequences of lt9a and lt9b were determined by the Edman degradation method. It has been suggested that both lt9a and lt9b are produced from the precursor encoded by the gene Lt9.1. During the conotoxin maturation process, different post-translational modifications occurred between lt9a and lt9b. Conotoxin lt9b was predicted to have two prolines that underwent hydroxylation and one glutamate that underwent carboxylation, while lt9a had no hydroxyproline and carboxyglutamate residue...
May 19, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28532810/species-level-identification-of-coagulase-negative-staphylococcus-spp-from-buffalo-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-cydb-real-time-quantitative-pcr
#13
Lucas J L Pizauro, Camila C de Almeida, Glenn A Soltes, Durda Slavic, Oswaldo D Rossi-Junior, Fernando A de Ávila, Luiz F Zafalon, Janet I MacInnes
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532539/-detection-of-serum-peptides-in-patients-with-lung-squamous-cell-carcinoma-by-maldi-tof-ms-and-analysis-of-their-correlation-with-chemotherapy-efficacy
#14
Guanhua Zhao, Bin Xu, Xiaoyan Li, Chuanhao Tang, Haifeng Qin, Hong Wang, Shaoxing Yang, Weixia Wang, Hongjun Gao, Kun He, Xiaoqing Liu
BACKGROUND: Treatment options for patients with squamous cell carcinoma of the lung (SCC) are limited in chemotherapy. However, not all patients could benefit form standard platinum regimen. Considering the dismal prognosis of patients with advanced SCC, a greater focus on selecting sensitive chemotherapy regimens remains of upmost importance to improve outcomes in this disease. In this study, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to detect pre-chemotherapy serum peptides in advanced lung squamous cell carcinoma patients accepting paclitaxel combined with platinum chemotherapy and to analyze the correlation between serum peptides and chemotherapy efficacy...
May 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28529026/the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-bacterial-identification-in-patients-with-smear-positive-bacterial-meningitis
#15
Boaz Bishop, Yuval Geffen, Adi Plaut, Ola Kassis, Roni Bitterman, Mical Paul, Ami Neuberger
OBJECTIVES: To assess the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in rapid identification of bacteria from smear-positive cerebrospinal fluid (CSF) in a cohort of patients with meningitis. METHODS: Single-center observational study, including adults and children with community-acquired or postneurosurgical bacterial meningitis. Meningitis was defined using established criteria. CSF samples that had a positive CSF Gram stain were directly examined by MALDI-TOF-MS...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28527668/imaging-mass-spectrometry-ims-of-cortical-lipids-from-preclinical-to-severe-stages-of-alzheimer-s-disease
#16
E Gónzalez de San Román, I Manuel, M T Giralt, I Ferrer, R Rodríguez-Puertas
Alzheimer's disease (AD) is a progressive neurodegenerative disease affecting millions of patients worldwide. Previous studies have demonstrated alterations in the lipid composition of lipid extracts from plasma and brain samples of AD patients. However, there is no consensus regarding the qualitative and quantitative changes of lipids in brains from AD patients. In addition, the recent developments in imaging mass spectrometry methods are leading to a new stage in the in situ analysis of lipid species in brain tissue slices from human postmortem samples...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28525645/molecular-characterization-of-prototheca-strains-isolated-in-china-revealed-the-first-cases-of-protothecosis-associated-with-prototheca-zopfii-genotype-1
#17
Noriyuki Hirose, Zhensheng Hua, Yuichi Kato, Qiangqiang Zhang, Ruoyu Li, Kazuko Nishimura, Michiaki Masuda
In this study, six strains of Prototheca isolated in China from human patients diagnosed as protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human protothecosis associated with P. zopfii genotype 1...
May 19, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28524084/bordetella-holmesii-lipid-a-structures-and-corresponding-genomic-sequences-comparison-in-three-clinical-isolates-and-the-reference-strain-atcc-51541
#18
Valérie Bouchez, Sami AlBitar-Nehmé, Alexey Novikov, Nicole Guiso, Martine Caroff
Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography-mass spectrometry (GC-MS), and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS)...
May 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28523576/proteomic-analysis-of-serum-amyloid-a-as-a-potential-marker-in-intestinal-beh%C3%A3-et-s-disease
#19
Hyun Jung Lee, Jae Hyun Kim, Seung Won Kim, Hyun Ah Joo, Hye Won Lee, You Sun Kim, Soo Jung Park, Sung Pil Hong, Tae Il Kim, Won Ho Kim, Young-Ho Kim, Jae Hee Cheon
BACKGROUND/AIMS: Data regarding biomarkers to understand disease pathogenesis and to assess disease activity of intestinal Behçet's disease (BD) are limited. Therefore, we aimed to investigate the differentially expressed proteins in sera from patients with intestinal BD and to search for biomarkers using mass spectrometry-based proteomic analysis. METHODS: Serum samples were pooled for the screening study, and two-dimensional electrophoresis (2-DE) was performed to characterize the proteins present in intestinal BD patients...
May 18, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28523506/maldi-mass-spectrometry-profiling-and-imaging-applied-to-the-analysis-of-latent-fingermarks
#20
Robert Bradshaw
Latent fingermarks are derived from a transfer of material from the fingertips to a surface upon contact. Traditionally, fingermarks are employed for biometric identification of individuals based on matching of the pattern of the ridges. However, in recent years, there has been a stark increase in the use of advanced analytical techniques in order to obtain additional information, specifically the chemical composition of the residue. Understanding the complexity of the endogenous and exogenous content of fingermarks could be extremely useful in allowing further development of enhancement techniques currently used in forensic scenarios by identifying potential target molecules...
2017: Methods in Molecular Biology
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