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MALDI and matrix

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https://www.readbyqxmd.com/read/28727449/the-use-of-maldi-ms-combined-with-differential-hydrogen-deuterium-exchange-for-semi-automated-protein-global-conformational-screening
#1
Gregory F Pirrone, Heather Wang, Nicole Canfield, Alexander S Chin, Timothy A Rhodes, Alexey A Makarov
Matrix-assisted laser desorption/ionization (MALDI) coupled with a time-of-flight (TOF) mass-spectrometry (MS) detector is acknowledged to be very useful for analysis of biological molecules. At the same time, hydrogen-deuterium exchange (HDX) is a well-known technique for studying protein higher-order structure. However, coupling MALDI with HDX may be challenging due to undesired back-exchange reactions during analysis. In this report, we survey an approach that utilizes MALDI coupled with automated sample preparation to compare global conformational changes of proteins under different solution conditions using differential HDX...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727417/oxygen-dependent-lipid-profiles-of-3d-cultured-human-chondrocytes-revealed-by-maldi-msi
#2
Brenda Bakker, Gert B Eijkel, Ron M A Heeren, Marcel Karperien, Janine N Post, Berta Cillero Pastor
Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supraphysiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (≤ 8%) promotes the chondrogenic phenotype. Exposure of cells to various oxygen levels alters their lipid metabolism, but detailed studies examining how hypoxia affects lipid metabolism in chondrocytes are lacking. To better understand the chondrocyte's behavior in response to oxygen we cultured 3D pellets of human primary chondrocytes in normoxia (20% oxygen) and hypoxia (2...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28726036/proteolytic-effects-of-gingipains-on-trefoil-factor-family-peptides
#3
Ponlatham Chaiyarit, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Barbara Potempa, Jan Potempa
OBJECTIVES: The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis. MATERIALS AND METHODS: Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease...
July 19, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28724284/inter-alpha-inhibitor-h4-as-a-potential-biomarker-predicting-the-treatment-outcomes-in-patients-with-hepatocellular-carcinoma
#4
Eun-Jung Lee, Seung-Hyun Yang, Kyoung-Jin Kim, Hyejung Cha, Seo Jin Lee, Ji-Hye Kim, Junkyu Song, Kyung-Hee Chun, Jinsil Seong
Purpose: Early prediction of treatment outcomes represents an essential step towards increased treatment efficacy and survival in patients with hepatocellular carcinoma (HCC). In this study, we performed two-dimensional electrophoresis (2-DE) followed by protein profiling to identify biomarkers predictive of therapeutic outcomes in patients with HCC who received liver-directed therapy (LDTx) involving local radiotherapy (RT), and studied the underlying mechanisms of the identified proteins...
July 17, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28722283/comparative-analysis-of-storage-conditions-and-homogenization-methods-for-tick-and-flea-species-for-identification-by-maldi-tof-ms
#5
A Nebbak, B El Hamzaoui, J-M Berenger, I Bitam, D Raoult, L Almeras, P Parola
Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months...
July 19, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28721611/bone-targeting-parathyroid-hormone-conjugates-outperform-unmodified-pth-in-the-anabolic-treatment-of-osteoporosis-in-rats
#6
Yang Yang, Ali Aghazadeh-Habashi, Arash Panahifar, Yuchin Wu, Krishna H Bhandari, Michael R Doschak
Synthetic parathyroid hormone (PTH) is clinically indicated for the treatment of osteoporosis, through its anabolic effects on parathyroid hormone receptors (PTHRs), located on osteoblast cells. However, the bioavailability of PTH for bone cells is restricted by the short half-life of PTH and the widespread distribution of PTHRs in non-skeletal tissues. To impart affinity for mineralized bone surfaces, bisphosphonate (BP)-mediated PTH analogues were synthesized, characterized, and evaluated in vitro and in vivo...
July 18, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28718648/multi-imaging-of-cytokinin-and-abscisic-acid-on-the-roots-of-rice-oryza-sativa-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#7
Katsuhiro Shiono, Riho Hashizaki, Toyofumi Nakanishi, Tatsuko Sakai, Takushi Yamamoto, Koretsugu Ogata, Ken-Ichi Harada, Hajime Ohtani, Hajime Katano, Shu Taira
Plant hormones are important mobile endogenous signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Visual information on more than one plant hormone from the same sample would be especially powerful, because it could enable rapid, intuitive interpretation. However, multi-imaging techniques for plant hormones have been unavailable. Here, we report the first multi-imaging of two plant hormones, one of cytokinin (i.e. trans-zeatin) and abscisic acid (ABA) by MALDI-TOF-MS imaging...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28716189/prediction-of-streptococcus-uberis-clinical-mastitis-risk-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-in-dairy-herds
#8
Simon C Archer, Andrew J Bradley, Selin Cooper, Peers L Davies, Martin J Green
The purpose of this study was to evaluate whether the risk of Streptococcus uberis clinical mastitis at cow level could be predicted from the historical presence of specific strains of S. uberis on dairy farms. Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to identify S. uberis isolates potentially capable of contagious transmission. Data were available from 10,652 cows from 52 English and Welsh dairy farms over a 14 month period, and 521 isolates of S. uberis from clinical mastitis cases were available for analysis...
September 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28713782/characterization-and-differentiation-of-mycobacterium-avium-subsp-paratuberculosis-from-other-mycobacteria-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#9
Subbarao V Ravva, Leslie A Harden, Chester Z Sarreal
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in cattle, is responsible for significant economic losses to the US dairy industry. The pathogen has also been associated with chronic human diseases like Crohn's disease, type 1 diabetes and multiple sclerosis. Determining causation requires rapid characterization and source tracking the pathogen. Here, we used matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to characterize and differentiate strains of MAP from 14 other species of Mycobacterium from bovine, human, and environmental sources...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28712153/proteomic%C3%A2-analysis-reveals-the-enhancement-of-human-serum-apolipoprotein-a-1-apo-a-1-in%C3%A2-individuals%C3%A2-infected-with-multiple-dengue-virus-serotypes
#10
Manchala Nageswar Reddy, Ranjeet Dungdung, Rajendra Pilankatta
OBJECTIVES: Human serum protein profiling of the individual infected with multiple dengue virus serotypes for identifying the potential biomarkers and to investigate the cause for the severity of dengue virus infection. METHODS: Dengue virus NS1 positive serum samples were pooled into two groups (S2 and S3) based on the molecular serotyping and number of heterotypic infections. The pooled serum samples were subjected to two dimensional gel electrophoresis (2DGE) to identify the differentially expressed proteins...
July 16, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28711436/a-simple-method-for-rapid-microbial-identification-from-positive-monomicrobial-blood-culture-bottles-through-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Jung-Fu Lin, Mao-Cheng Ge, Tsui-Ping Liu, Shih-Cheng Chang, Jang-Jih Lu
BACKGROUND AND PURPOSE: Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. METHODS: We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis...
June 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28707193/laser-pulse-width-dependence-and-ionization-mechanism-of-matrix-assisted-laser-desorption-ionization
#12
Sheng-Ping Liang, I-Chung Lu, Shang-Ting Tsai, Jien-Lian Chen, Yuan Tseh Lee, Chi-Kung Ni
Ultraviolet laser pulses at 355 nm with variable pulse widths in the region from 170 ps to 1.5 ns were used to investigate the ionization mechanism of matrix-assisted laser desorption/ionization (MALDI) for matrices 2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA), and sinapinic acid (SA). The mass spectra of desorbed ions and the intensity and velocity distribution of desorbed neutrals were measured simultaneously for each laser shot. These quantities were found to be independent of the laser pulse width...
July 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28706514/evaluation-of-the-bruker-biotyper-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-identification-of-aspergillus-species-directly-from-growth-on-solid-agar-media
#13
Ying Li, He Wang, Yu-Pei Zhao, Ying-Chun Xu, Po-Ren Hsueh
We evaluated the accuracy of the Bruker Biotyper matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) system at identifying clinical isolates of Aspergillus species that were grown on agar media. A total of 381 non-duplicate Aspergillus isolates representing 21 different Aspergillus species identified by molecular analysis were included in this study. The Bruker Biotyper MALDI-TOF MS system was able to identify 30.2% (115/381) of the isolates to the species level (score values of ≥2...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28706253/affinity-purification-of-erythropoietin-from-cell-culture-supernatant-combined-with-maldi-tof-ms-analysis-of-erythropoietin-n-glycosylation
#14
David Falck, Markus Haberger, Rosina Plomp, Michaela Hook, Patrick Bulau, Manfred Wuhrer, Dietmar Reusch
Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from cell culture supernatant (CCS) in approximately 24 h, offers the possibility to follow changes during production. To address this challenge, we present a complete workflow consisting of protein purification, glycan release, sialic acid derivatization, solid phase extraction, matrix-assisted laser desorption/ionization - mass spectrometry (MALDI-MS) analysis and MassyTools data processing...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28702487/dataset-on-lipid-profile-of-bovine-oocytes-exposed-to-l%C3%AE-phosphatidylcholine-during-in-vitro-maturation-investigated-by-maldi-mass-spectrometry-and-gas-chromatography-flame-ionization-detection
#15
Alessandra A Vireque, Christina R Ferreira, Rafael R Hatanaka, Alessandra Tata, Katia Roberta A Belaz, Vanessa G Santos, Marcos N Eberlin, Marcos Felipe Silva de Sá, Rui A Ferriani, Ana Carolina J S Rosa E Silva
Data presented in this article are related with the research article entitled "Effect of soybean phosphatidylcholine on lipid profile of bovine oocytes matured in vitro" [1]. This article describes the differences in the relative abundance of the lipid ions detected by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in control and Lα-phosphatidylcholine-treated oocytes. In addition, the fatty acids (FA) content in pure Lα-phosphatidylcholine supplement and oocytes was analyzed by gas chromatography-flame ionization detection (GC-FID)...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28695531/gain-switching-for-a-detection-system-to-accommodate-a-newly-developed-maldi-based-quantification-method
#16
Sung Hee Ahn, Taeghwan Hyeon, Myung Soo Kim, Jeong Hee Moon
In matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF), matrix-derived ions are routinely deflected away to avoid problems with ion detection. This, however, limits the use of a quantification method that utilizes the analyte-to-matrix ion abundance ratio. In this work, we will show that it is possible to measure this ratio by a minor instrumental modification of a simple form of MALDI-TOF. This involves detector gain switching. Graphical Abstract ᅟ.
July 10, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28693729/sialic-acid-linkage-specific-permethylation-for-improved-profiling-of-protein-glycosylation-by-maldi-tof-ms
#17
Kuan Jiang, He Zhu, Lei Li, Yuxi Guo, Ebtesam Gashash, Cheng Ma, Xiaolin Sun, Jing Li, Lianwen Zhang, Peng George Wang
Protein glycosylation mediates a wide range of cellular processes, affecting development and disease in mammals. Deciphering the "glycocodes" requires rapid, sensitive and in-depth characterization of diverse glycan structures derived from biological samples. In this study, we described a two-step derivatization strategy termed linkage-specific sialic acid permethylation (SSAP) consisting of dimethylamination and permethylation for the improved profiling of glycosylation by matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28692323/identifying-inhibitors-of-inflammation-a-novel-high-throughput-maldi-tof-screening-assay-for-salt-inducible-kinases-siks
#18
Rachel E Heap, Anthony G Hope, Lesley-Anne Pearson, Kathleen M S E Reyskens, Stuart P McElroy, C James Hastie, David W Porter, J Simon C Arthur, David W Gray, Matthias Trost
Matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry has become a promising alternative for high-throughput drug discovery as new instruments offer high speed, flexibility and sensitivity, and the ability to measure physiological substrates label free. Here we developed and applied high-throughput MALDI TOF mass spectrometry to identify inhibitors of the salt-inducible kinase (SIK) family, which are interesting drug targets in the field of inflammatory disease as they control production of the anti-inflammatory cytokine interleukin-10 (IL-10) in macrophages...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28692093/chemoselective-ligation-reaction-of-n-acetylglucosamine-nag-with-hydrazide-functional-probes-to-determine-galactosyltransferase-activity-by-maldi-mass-spectrometry
#19
Hyojik Yang, Quan Cheng
Quantification of β-1,4-galactosyltransferase (β-1,4-GT) activity is of considerable significance in the diagnosis of various cancers including lung and ovarian cancer. We report here the use of synthetic β-N-acetylglucosamine (NAG) ligands that contain hydrazide functional groups to determine galactosyltransferase activity by mass spectrometry. With hydrazide-linked β-d-NAG as the acceptor, the activity of β-1,4-GT is quantified by matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF) mass spectrometry (MS) with high efficiency...
July 10, 2017: Analyst
https://www.readbyqxmd.com/read/28691891/incidence-of-oxa-23-and-oxa-58-carbapenemases-coexpressed-in-clinical-isolates-of-acinetobacter-baumannii-in-tunisia
#20
Najla Mathlouthi, Yomna Ben Lamine, Rania Somai, Sophia Bouhalila-Besbes, Sofiane Bakour, Jean-Marc Rolain, Chedly Chouchani
Acinetobacter baumannii is an important opportunistic and multidrug-resistant pathogen responsible for nosocomial infections in health facilities. The aim of this study was to characterize the molecular mechanisms of carbapenem resistance in A. baumannii isolates isolated from Mohamed Kassab Orthopedic Institute in Tunis, Tunisia. Twenty-five imipenem-resistant A. baumannii clinical isolates collected between 2013 and 2016 were identified using API 20NE and were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS)...
July 10, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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