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https://www.readbyqxmd.com/read/28806859/analysis-of-low-molecular-weight-compounds-using-maldi-and-ldi-tof-ms-direct-detection-of-active-pharmaceutical-ingredients-in-different-formulations
#1
João L Bronzel, Cintia D F Milagre, Humberto M S Milagre
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) is a high throughput, easy to use analytical technique. The simple sample preparation of this technique and its tolerance to the presence of contaminants are among its advantages. In contrast, depending on the matrix used, MALDI can ionize and generates ions in the low m/z range that complicate the interpretation of the spectra of low molecular weight compounds. To address this issue, one can envisage the use of tunable ionic matrices that can reduce the low m/z interferents...
August 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28805727/leaf-physiological-and-proteomic-analysis-to-elucidate-silicon-induced-adaptive-response-under-salt-stress-in-rosa-hybrida-rock-fire
#2
Prabhakaran Soundararajan, Abinaya Manivannan, Chung Ho Ko, Sowbiya Muneer, Byoung Ryong Jeong
Beneficial effects of silicon (Si) on growth and development have been witnessed in several plants. Nevertheless, studies on roses are merely reported. Therefore, the present investigation was carried out to illustrate the impact of Si on photosynthesis, antioxidant defense and leaf proteome of rose under salinity stress. In vitro-grown, acclimatized Rosa hybrida 'Rock Fire' were hydroponically treated with four treatments, such as control, Si (1.8 mM), NaCl (50 mM), and Si+NaCl. After 15 days, the consequences of salinity stress and the response of Si addition were analyzed...
August 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28803462/structural-characterization-of-methylenedianiline-regioisomers-by-ion-mobility-and-mass-spectrometry-iii-maldi-spectra-of-2-ring-isomers
#3
Sarah M Stow, Tiffany M Crescentini, Jay G Forsythe, Jody C May, John A McLean, David M Hercules
Characterization of methylenedianiline (MDA) 2-ring isomers (2,2'-, 2,4'- and 4,4'-MDA) is reported using matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS), a common technique used for characterizing synthetic polymers. MDA is a precursor to methylene diphenyl diisocyanate (MDI) a hard block component in polyurethane (PUR) synthesis. This manuscript focuses on comparing MALDI results to those of our previous electrospray ionization-mass spectrometry (ESI-MS) studies. In ESI, 2-ring MDA isomers formed single unique [M+H]+ (199 Da) parent ions, whereas in MALDI each isomer shows significant formation of three precursor ions: [M-H]+ = 197 Da, [M...
August 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28803337/production-of-rhamnolipids-by-semi-solid-state-fermentation-with-pseudomonas-aeruginosa-rg18-for-heavy-metal-desorption
#4
Jianrong Wu, Jingbo Zhang, Panpan Wang, Li Zhu, Minjie Gao, Zhiyong Zheng, Xiaobei Zhan
Foaming problem and cost of substrate limit the commercial application of rhamnolipids, a potential biosurfactant produced by Pseudomonas aeruginosa. We explored the production of rhamnolipids by a semi-solid-state (SSS) fermentation strategy with glycerol as carbon source and rapeseed/wheat bran as matrix, along with the capacity of the produced rhamnolipids to solubilize lead and cadmium in aqueous solution. Structural analysis by MALDI-TOF MS indicated the increased proportion of mono-rhamnolipids from SSS fermentation...
August 12, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28802703/identification-and-characterization-of-clostridium-botulinum-group-iii-field-strains-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#5
Luca Bano, Ilenia Drigo, Elena Tonon, Simone Pascoletti, Cinzia Puiatti, Fabrizio Anniballi, Bruna Auricchio, Florigio Lista, Cesare Montecucco, Fabrizio Agnoletti
Animal botulism is primarily due to botulinum neurotoxin (BoNT) types C, D or their chimeric variants C/D or D/C, produced by Clostridium botulinum group III, which appears to include the genetically indistinguishable Clostridium haemolyticum and Clostridium novyi. In the present study, we used matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI TOF MS) to identify and characterize 81 BoNT-producing Clostridia isolated in 47 episodes of animal botulism. The instrument's default database, containing no entries for Clostridium botulinum, permitted reliable identification of 26 strains at the genus level...
August 9, 2017: Anaerobe
https://www.readbyqxmd.com/read/28801933/maldi-imaging-guided-microproteomic-analyses-of-heterogeneous-breast-tumors-a-pilot-study
#6
Deborah Alberts, Charles Pottier, Nicolas Smargiasso, Dominique Baiwir, Gabriel Mazzucchelli, Philippe Delvenne, Mark Kriegsmann, Daniel Kazdal, Arne Warth, Edwin De Pauw, Rémi Longuespée
PURPOSE: Matrix-assisted laser desorption/ionization (MALDI) imaging is an ideal tool to study intratumor heterogeneity (ITH) and its implication in prognostic stratification of patients. However there are some drawbacks concerning protein identification. On the other hand, laser microdissection (LMD)-based microproteomics allows retrieving thousands of protein identifications from small tissue pieces. As a proof of concept, we combined these two complementary approaches to analyze heterogeneous regions in breast tumors...
August 11, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28801836/optimization-of-sample-preparation-and-instrumental-parameters-for-the-rapid-analysis-of-drugs-of-abuse-in-hair-samples-by-maldi-ms-ms-imaging
#7
Bryn Flinders, Emma Beasley, Ricky M Verlaan, Eva Cuypers, Simona Francese, Tom Bassindale, Malcolm R Clench, Ron M A Heeren
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) has been employed to rapidly screen longitudinally sectioned drug user hair samples for cocaine and its metabolites using continuous raster imaging. Optimization of the spatial resolution and raster speed were performed on intact cocaine contaminated hair samples. The optimized settings (100 × 150 μm at 0.24 mm/s) were subsequently used to examine longitudinally sectioned drug user hair samples. The MALDI-MS/MS images showed the distribution of the most abundant cocaine product ion at m/z 182...
August 11, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28801830/cloning-and-expression-analysis-of-human-amelogenin-in-nicotiana-benthamiana-plants-by-means-of-a-transient-expression-system
#8
Mattia Pegoraro, Slavica Matić, Barbara Pergolizzi, Luca Iannarelli, Andrea M Rossi, Marco Morra, Emanuela Noris
Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxyapatite deposit, and regenerative functions in bone and vascular tissue and in wound healing. Biotechnological products containing amelogenin seem to facilitate these processes. Here, we describe the production of human amelogenin in plants by transient transformation of Nicotiana benthamiana with constructs carrying synthetic genes with optimized human or plant codons...
August 11, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28801789/multimodal-laser-ablation-desorption-imaging-analysis-of-zn-and-mmp-11-in-breast-tissues
#9
Raquel González de Vega, María Luisa Fernández Sanchez, Noemí Eiro, Francisco J Vizoso, Michael Sperling, Uwe Karst, Alfredo Sanz Medel
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases. The main functions of these metalloproteinases are the degradation of the stromal connective tissue and basement membrane components. Recent data from model systems suggest that MMPs are involved in breast cancer (BC) initiation, invasion, and metastasis. Particularly, MMP-11 (stromelysin-3) is expressed in stromal fibroblasts adjacent to epithelial tumor cells, and high levels of this metalloproteinase were associated with tumor progression and poor prognosis of BC...
August 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801691/a-new-data-processing-routine-facilitating-the-identification-of-surface-adhered-proteins-from-bacterial-conditioning-films-via-qcm-d-maldi-tof-ms
#10
Siegfried Hohmann, Anke Neidig, Boris Kühl, Frank Kirschhöfer, Jörg Overhage, Gerald Brenner-Weiß
Conditioning films are an important factor in the initiation and development of microbial biofilms, which are the leading cause of chronic infections associated with medical devices. Here, we analyzed the protein content of conditioning films formed after exposure to supernatants of cultures of the human pathogen Pseudomonas aeruginosa PAO1. Adhesion of substances from the supernatant was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) sensor chips modified with the commonly used implant material titanium dioxide (TiO2)...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28800704/qualitative-and-quantitative-analysis-of-carbohydrate-modification-on-glycoproteins-from-seeds-of-ginkgo-biloba
#11
Ting Wang, Xiao-Chun Hu, Zhi-Peng Cai, Josef Voglmeir, Li Liu
Recent progress in the relationship between carbohydrate cross-reactive determinants (CCDs) and allergic response highlights the importance of carbohydrate moieties in innate immune system. A previous research pointed out that the protein allergen in Ginkgo biloba seeds are glycosylated and the oligosaccharides conjugated to these proteins might also contribute to the allergy. The aim of this study was to analyze carbohydrate moieties, especially the N-linked glycans, of glycoproteins from Ginkgo seeds originated from different places for detailed structures, to enable further research on the roles that N-glycans play in Ginkgo-caused allergy...
August 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28800445/enzyme-sensitive-gemcitabine-conjugated-albumin-nanoparticles-as-a-versatile-theranostic-nanoplatform-for-pancreatic-cancer-treatment
#12
Haijie Han, Jinhui Wang, Tingting Chen, Lichen Yin, Qiao Jin, Jian Ji
Development of gemcitabine (GEM) nanocarriers as theranostic agents for pancreatic cancer chemotherapy has received extensive attention in recent years. A novel enzyme-sensitive albumin-based GEM delivery nanoplatform was developed in this research by simple conjugation of GEM to human serum albumin (HSA) via cathepsin B cleavable peptide GFLG and then complexing with near-infrared (NIR) dye IR780, forming a HSA-GEM/IR780 complex. The successful preparation of HSA-GEM/IR780 complex was confirmed by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS), UV-vis-NIR absorption spectra and fluorescent emission spectra...
July 17, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28797803/a-multi-center-ring-trial-for-the-identification-of-anaerobic-bacteria-using-maldi-tof-ms
#13
A C M Veloo, H Jean-Pierre, U S Justesen, T Morris, E Urban, I Wybo, H N Shah, A W Friedrich, T Morris, H N Shah, H Jean-Pierre, U S Justesen, E Nagy, E Urban, M Kostrzewa, A Veloo, A W Friedrich
Inter-laboratory reproducibility of Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of anaerobic bacteria has not been shown before. Therefore, ten anonymized anaerobic strains were sent to seven participating laboratories, an initiative of the European Network for the Rapid Identification of Anaerobes (ENRIA). On arrival the strains were cultured and identified using MALDI-TOF MS. The spectra derived were compared with two different Biotyper MALDI-TOF MS databases, the db5627 and the db6903...
August 7, 2017: Anaerobe
https://www.readbyqxmd.com/read/28796186/maldi-tof-ms-for-the-identification-of-cultivable-organic-degrading-bacteria-in-contaminated-groundwater-near-unconventional-natural-gas-extraction-sites
#14
Inês C Santos, Misty S Martin, Doug D Carlton, Catarina L Amorim, Paula M L Castro, Zacariah L Hildenbrand, Kevin A Schug
Groundwater quality and quantity is of extreme importance as it is a source of drinking water in the United States. One major concern has emerged due to the possible contamination of groundwater from unconventional oil and natural gas extraction activities. Recent studies have been performed to understand if these activities are causing groundwater contamination, particularly with respect to exogenous hydrocarbons and volatile organic compounds. The impact of contaminants on microbial ecology is an area to be explored as alternatives for water treatment are necessary...
August 10, 2017: Microorganisms
https://www.readbyqxmd.com/read/28794918/sensitive-and-structure-informative-n-glycosylation-analysis-by-maldi-ms-ionization-fragmentation-and-derivatization
#15
Takashi Nishikaze
Mass spectrometry (MS) has become an indispensable tool for analyzing post translational modifications of proteins, including N-glycosylated molecules. Because most glycosylation sites carry a multitude of glycans, referred to as "glycoforms," the purpose of an N-glycosylation analysis is glycoform profiling and glycosylation site mapping. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has unique characteristics that are suited for the sensitive analysis of N-glycosylated products...
2017: Mass Spectrometry
https://www.readbyqxmd.com/read/28794317/high-resolution-kendrick-mass-defect-analysis-of-poly-ethylene-oxide-based-non-ionic-surfactants-and-their-degradation-products
#16
Thierry Fouquet, Haruo Shimada, Katsuyuki Maeno, Kanako Ito, Yuka Ozeki, Shinya Kitagawa, Hajime Ohtani, Hiroaki Sato
Matrix assisted laser desorption ionization (MALDI) high-resolution mass spectrometry (HRMS) and the recently introduced high-resolution Kendrick mass defect (HRKMD) analysis are combined to thoroughly characterize non-ionic surfactants made of a poly(ethylene oxide) (PEO) core capped by esters of fatty acids. A PEO monostearate surfactant is first analyzed as a proof of principle of the HRKMD analysis conducted with a fraction of EO as the base unit (EO/X with X being an integer) in lieu of EO for a regular KMD analysis...
August 8, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28792944/maldi-tof-ms-typing-enables-the-classification-of-brewing-yeasts-of-the-genus-saccharomyces-to-major-beer-styles
#17
Alexander Lauterbach, Julia C Usbeck, Jürgen Behr, Rudi F Vogel
Brewing yeasts of the genus Saccharomyces are either available from yeast distributor centers or from breweries employing their own "in-house strains". During the last years, the classification and characterization of yeasts of the genus Saccharomyces was achieved by using biochemical and DNA-based methods. The current lack of fast, cost-effective and simple methods to classify brewing yeasts to a beer type, may be closed by Matrix Assisted Laser Desorption/Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) upon establishment of a database based on sub-proteome spectra from reference strains of brewing yeasts...
2017: PloS One
https://www.readbyqxmd.com/read/28792758/profiling-of-microbial-colonies-for-high-throughput-engineering-of-multi-step-enzymatic-reactions-via-optically-guided-maldi-ms
#18
Tong Si, Bin Li, Troy J Comi, Yuwei Wu, Pingfan Hu, Yuying Wu, Yuhao Min, Douglas A Mitchell, Huimin Zhao, Jonathan V Sweedler
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry (MS) imaging has been used for rapid phenotyping of enzymatic activities, but is mainly limited to single-step conversions. Herein we report a label-free method for high-throughput engineering of multi-step biochemical reactions based on optically guided MALDI-ToF MS analysis of bacterial colonies. The bacterial cells provide containment of multiple enzymes and access to substrates and cofactors via metabolism. Automated MALDI-ToF MS acquisition from randomly distributed colonies simplifies procedures to prepare strain libraries without liquid handling...
August 9, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28783195/murine-cutaneous-leishmaniasis-investigated-by-maldi-mass-spectrometry-imaging
#19
Fernanda Negrão, Daniele F de O Rocha, Caroline F Jaeeger, Francisca J S Rocha, Marcos N Eberlin, Selma Giorgio
Imaging mass spectrometry (IMS) is recognized as a powerful tool to investigate the spatial distribution of untargeted or targeted molecules of a wide variety of samples including tissue sections. Leishmania is a protozoan parasite that causes different clinical manifestations in mammalian hosts. Leishmaniasis is a major public health risk in different continents and represents one of the most important neglected diseases. Cutaneous lesions from mice experimentally infected with Leishmania spp. were investigated by matrix-assisted laser desorption ionization MS using the SCiLS Lab software for statistical analysis...
August 7, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28783081/culturing-toxic-benthic-blooms-the-fate-of-natural-biofilms-in-a-microcosm-system
#20
Francesca Di Pippo, Roberta Congestri
A microcosm designed for culturing aquatic phototrophic biofilms on artificial substrata was used to perform experiments with microphytobenthos sampled during summer toxic outbreaks of Ostreopsis cf. ovata along the Middle Tyrrhenian coast. This dynamic approach aimed at exploring the unique and complex nature of O. cf. ovata bloom development in the benthic system. Epibenthic assemblages were used as inocula for co-cultures of bloom organisms on polycarbonate slides at controlled environmental conditions. Biofilm surface adhesion, growth, and spatial structure were evaluated along with shifts in composition and matrix production in a low disturbance regime, simulating source habitat...
August 6, 2017: Microorganisms
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