keyword
MENU ▼
Read by QxMD icon Read
search

Beta cells from Alpha cells

keyword
https://www.readbyqxmd.com/read/29160393/pro-inflammatory-analysis-of-macrophages-in-contact-with-titanium-particles-and-porphyromonas-gingivalis
#1
Cindy Goes Dodo, Luiz Meirelles, Alejandro Aviles-Reyes, Karina Gonzalez Silvério Ruiz, Jacqueline Abranches, Altair Antoninha Del Bel Cury
During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS)...
July 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29155215/membrane-estrogen-receptors-signal-to-determine-transcription-factor-function
#2
Ellis R Levin
Estrogen receptors (ER) alpha and beta as well as many other steroid receptors are found both within the nucleus and outside the nucleus. This includes extra-nuclear receptors in many organelles, including mitochondria, endoplasmic reticulum, cytosolic endosomes, and membrane lipid rafts, such as caveolae. The functions of these receptors in the various extra-nuclear cell organelles are not well described, but progress for understanding steroid receptor signaling from the membrane has advanced. In this Review I will discuss the enlarging role of membrane ER signaling to the expression, cell localization, and function of transcription factors that are essential to mediate cell physiology or pathophysiology in many organs...
November 16, 2017: Steroids
https://www.readbyqxmd.com/read/29147967/association-of-inflammatory-bowel-disease-with-arthritis-evidence-from-in-silico-gene-expression-patterns-and-network-topological-analysis
#3
Anukriti Verma, Pallavi Somvanshi, Shafiul Haque, Bhawna Rathi, Shivani Sharda
Inflammatory bowel disease (IBD) is an idiopathic prolonged ailment accountable for inflammatory conditions of the intestine. Moreover, arthritis is responsible for joints' stiffness and painful inflammation. IBD shows certain articular extra-intestinal manifestations associating IBD with arthritis. IBD associated arthritis is found to be linked with ankylosing spondylitis (AS). The present study insights for the potential and putative drug targets and biomarkers of IBD associated with arthritis using in silico approaches...
November 17, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/29142404/chalepin-a-compound-from-ruta-angustifolia-l-pers-exhibits-cell-cycle-arrest-at-s-phase-suppresses-nuclear-factor-kappa-b-nf-%C3%AE%C2%BAb-pathway-signal-transducer-and-activation-of-transcription-3-stat3-phosphorylation-and-extrinsic-apoptotic-pathway-in-non-small-cell
#4
Jaime Stella Moses Richardson, Norhaniza Aminudin, Sri Nurestri Abd Malek
Background: Plants have been a major source of inspiration in developing novel drug compounds in the treatment of various diseases that afflict human beings worldwide. Ruta angustifolia L. Pers known locally as Garuda has been conventionally used for various medicinal purposes such as in the treatment of cancer. Objective: A dihydrofuranocoumarin named chalepin, which was isolated from the chloroform extract of the plant, was tested on its ability to inhibit molecular pathways of human lung carcinoma (A549) cells...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29140156/conditioned-medium-from-human-gingival-mesenchymal-stem-cells-protects-motor-neuron-like-nsc-34-cells-against-scratch-injury-induced-cell-death
#5
Thangavelu Soundara Rajan, Francesca Diomede, Placido Bramanti, Oriana Trubiani, Emanuela Mazzon
Neuronal cell death is a normal process during central nervous system (CNS) development and is also involved in the death of motor neurons in diverse spinal motor neuron degenerative diseases. Here, we investigated the neuroprotective effect of secretory factors released from human gingival mesenchymal stem cells (hGMSCs) in mechanically injured murine motor-neuron-like NSC-34 cells. The cells were exposed to scratch injury and the markers for apoptosis and oxidative stress were examined. Immunocytochemistry results showed that proapoptotic markers cleaved caspase-3 and Bax were elevated while anti-apoptotic protein Bcl-2 was suppressed in scratch-injured NSC-34 cells...
November 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/29136347/pro-inflammatory-mediator-responses-from-neonatal-airway-epithelial-cells-and-early-childhood-wheeze
#6
Steve Turner, David Miller, Garry M Walsh, Alison Scaife, Ultan F Power, Michael D Shields, Graham Devereux
BACKGROUND: Airway epithelial cell (AEC) function differs between children with and without asthma. Here, we associated neonatal AEC function with asthma symptoms at 4 years of age. METHODS: Nasal AEC were collected from neonates within 48 h of birth. Cells were cultured and stimulated with tumor necrosis factor alpha/interleukin-1 beta (TNFα/IL-1β), lipopolysaccharide (LPS), or house dust mite (HDM). Absolute concentrations of pro-inflammatory mediators in the culture supernatant were quantified and expressed as median [interquartile range] in pg/mg protein...
November 14, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29129744/upregulation-of-aquaporin-3-expression-by-diterpenoids-in-euphorbia-pekinensis-is-associated-with-activation-of-the-nf-%C3%AE%C2%BAb-signaling-pathway-in-the-co-culture-system-of-ht-29-and-raw-264-7%C3%A2-cells
#7
Hongli Yu, Lian Liu, Kuilong Wang, Hao Wu, Wei Wang, Xingde Zhang, Guojing Cui, Xiaobing Cui, Jianyu Huang
This study was designed to evaluate the toxic effects of diterpenoids separated from the roots of Euphorbia pekinensis, a type of widely used traditional Chinese medicine. This herb has intestinal toxicity associated with its complex diterpenoids. In this study, the diterpenoids (pekinenin A, pekinenin C, pekinenin F, pekinenin G, yuexiandajisu A, (-)-(1S)-15-hydroxy-18-carboxycembrene) elevated the expression of interleukin 1 beta and tumor necrosis factor alpha in a dose-dependent manner at doses of 6.25, 12...
November 9, 2017: Biochimie
https://www.readbyqxmd.com/read/29129393/disulfide-bond-mediated-cross-linking-of-corneous-beta-proteins-in-lepidosaurian-epidermis
#8
Karin B Holthaus, Lorenzo Alibardi
Corneous beta-proteins (CBPs), formerly referred to as beta-keratins, are major protein components of the epidermis in lepidosaurian reptiles and are largely responsible for their material properties. These proteins have been suggested to form filaments of 3.4nm in thickness and to interact with themselves or with other proteins, including intermediate filament (IF) keratins. Here, we performed immunocytochemical labeling of CBPs in the epidermis of different lizards and snakes and investigated by immunoblotting analysis whether the reduction of disulfide bonds or protein oxidation affects the solubility and mobility of these CBPs...
October 29, 2017: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/29123113/talen-mediated-functional-correction-of-human-ipsc-derived-macrophages-in-context-of-hereditary-pulmonary-alveolar-proteinosis
#9
Alexandra Kuhn, Mania Ackermann, Claudio Mussolino, Toni Cathomen, Nico Lachmann, Thomas Moritz
Hereditary pulmonary alveolar proteinosis (herPAP) constitutes a rare, life threatening lung disease characterized by the inability of alveolar macrophages to clear the alveolar airspaces from surfactant phospholipids. On a molecular level, the disorder is defined by a defect in the CSF2RA gene coding for the GM-CSF receptor alpha-chain (CD116). As therapeutic options are limited, we currently pursue a cell and gene therapy approach aiming for the intrapulmonary transplantation of gene-corrected macrophages derived from herPAP-specific induced pluripotent stem cells (herPAP-iPSC) employing transcriptional activator-like effector nucleases (TALENs)...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29121068/metabolic-regulation-of-glp-1-and-pc1-3-in-pancreatic-%C3%AE-cell-line
#10
Veronica Sancho, Giuseppe Daniele, Daniela Lucchesi, Roberto Lupi, Annamaria Ciccarone, Giuseppe Penno, Cristina Bianchi, Angela Dardano, Roberto Miccoli, Stefano Del Prato
BACKGROUND AND AIMS: An intra-islet incretin system has been recently suggested to operate through modulation of the expression and activity of proconvertase 1/3 and 2 (PC1/3, PC2) in pancreatic alpha-cell accounting for local release of GLP-1. Little is known, whether this alpha-cell activity can be affected by the metabolic alterations occurring in type 2 diabetes, such as hyperglycemia, hyperlipidemia or hyperglucagonemia. MATERIALS AND METHODS: AlphaTC1/6 cells from a mice pancreatic cell line were incubated in the presence of two glucose (G) concentration (5...
2017: PloS One
https://www.readbyqxmd.com/read/29111540/transcription-and-regulation-of-hepatitis-b-virus-genes-in-host-sperm-cells
#11
Ying Zhong, Dong-Ling Liu, Mohamed Morsi M Ahmed, Peng-Hao Li, Xiao-Ling Zhou, Qing-Dong Xie, Xiao-Qing Xu, Ting-Ting Han, Zhi-Wei Hou, Ji-Hua Huang, Lan Xu, Tian-Hua Huang
To investigate whether transcription of hepatitis B virus (HBV) gene occurs in human sperm, total RNA was extracted from sperm of patients with chronic HBV infection (test-1), from donor sperm transfected with a plasmid containing the full-length HBV genome (test-2), and from nontransfected donor sperm (control), used as the template for reverse transcription-polymerase chain reaction (RT-PCR). Positive bands for HBV DNA were observed in the test groups but not in the control. Next, to identify the role of host genes in regulating viral gene transcription in sperm, total RNA was extracted from 2-cell embryos derived from hamster oocytes fertilized in vitro by HBV-transfected (test) or nontransfected (control) human sperm and successively subjected to SMART-PCR, suppression subtractive hybridization, T/A cloning, bacterial amplification, microarray hybridization, sequencing and the Basic Local Alignment Search Tool (BLAST) search to isolate differentially expressed genes...
November 7, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29100082/topology-of-membrane-proteins-predictions-limitations-and-variations
#12
REVIEW
Konstantinos D Tsirigos, Sudha Govindarajan, Claudio Bassot, Åke Västermark, John Lamb, Nanjiang Shu, Arne Elofsson
Transmembrane proteins perform a variety of important biological functions necessary for the survival and growth of the cells. Membrane proteins are built up by transmembrane segments that span the lipid bilayer. The segments can either be in the form of hydrophobic alpha-helices or beta-sheets which create a barrel. A fundamental aspect of the structure of transmembrane proteins is the membrane topology, that is, the number of transmembrane segments, their position in the protein sequence and their orientation in the membrane...
October 26, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/29074617/organelle-specific-single-molecule-imaging-of-%C3%AE-4%C3%AE-2-nicotinic-receptors-reveals-the-effect-of-nicotine-on-receptor-assembly-and-cell-surface-trafficking
#13
Ashley M Fox-Loe, Faruk H Moonschi, Christopher I Richards
Nicotinic acetylcholine receptors (nAChRs) assemble in the endoplasmic reticulum (ER) and traffic to the cell surface as pentamers composed of alpha and beta subunits. Many nAChR subtypes can assemble with varying subunit ratios, giving rise to multiple stoichiometries exhibiting different subcellular localization and functional properties. In addition to the endogenous neurotransmitter acetylcholine, nicotine also binds and activates nAChRs and influences their trafficking and expression on the cell surface...
October 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29070693/cryo-em-structure-of-porcine-delta-coronavirus-spike-protein-in-the-pre-fusion-state
#14
Jian Shang, Yuan Zheng, Yang Yang, Chang Liu, Qibin Geng, Wanbo Tai, Lanying Du, Yusen Zhou, Wei Zhang, Fang Li
Coronavirus spike proteins from different genera are divergent, although they all mediate coronavirus entry into cells by binding to host receptors and fusing viral and cell membranes. Here we determined the cryo-EM structure of porcine delta coronavirus (PdCoV) spike protein at 3.3-angstrom resolution. The trimeric protein contains three receptor-binding S1 subunits that tightly pack into a crown-like structure and three membrane-fusion S2 subunits that form a stalk. Each S1 subunit contains two domains, N-terminal domain (S1-NTD) and C-terminal domain (S1-CTD)...
October 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29056891/polygala-tenuifolia-extract-inhibits-lipid-accumulation-in-3t3-l1-adipocytes-and-high-fat-diet-induced-obese-mouse-model-and-affects-hepatic-transcriptome-and-gut-microbiota-profiles
#15
Chun-Chung Wang, Jui-Hung Yen, Yi-Cheng Cheng, Chia-Yu Lin, Cheng-Ta Hsieh, Rung-Jiun Gau, Shu-Jiau Chiou, Hwan-You Chang
Obesity, the excessive accumulation of lipids in the body, is closely associated with many prevalent human disorders. Continued efforts to identify plant extracts that exhibit anti-obesity effects have drawn much attention. This study investigated whether a Polygala tenuifolia extract (PTE) possesses anti-obesity activity and how PTE may affect liver gene expression and gut microbiota. We used 3T3-L1 adipocytes and a high-fat diet-induced obese mouse model to determine the effects of PTE on lipid accumulation...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/29046453/murine-hepatitis-virus-nsp14-exoribonuclease-activity-is-required-for-resistance-to-innate-immunity
#16
James Brett Case, Yize Li, Ruth Elliott, Xiaotao Lu, Kevin W Graepel, Nicole R Sexton, Everett Clinton Smith, Susan R Weiss, Mark R Denison
Coronaviruses (CoVs) are positive-sense RNA viruses that infect numerous mammalian and avian species and are capable of causing severe and lethal disease in humans. CoVs encode several innate immune antagonists that counteract the host innate immune response to facilitate efficient viral replication. CoV non-structural protein 14 (nsp14) encodes 3' -to-5' exoribonuclease activity (ExoN), which performs a proofreading function and is required for high-fidelity replication. Outside of the order Nidovirales, arenaviruses are the only RNA viruses that encode an ExoN, which functions to degrade dsRNA replication intermediates...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046441/structure-function-dissection-of-the-pseudorabies-virus-glycoprotein-b-fusion-loops
#17
Melina Vallbracht, Delphine Brun, Matteo Tassinari, Marie-Christine Vaney, Gérard Pehau-Arnaudet, Pablo Guardado-Calvo, Ahmed Haouz, Barbara G Klupp, Thomas C Mettenleiter, Felix A Rey, Marija Backovic
Conserved across the Herpesviridae family, glycoprotein B (gB) is responsible for driving fusion of the viral envelope with the host cell membrane for entry upon receptor binding and activation by the viral gH/gL complex. Although crystal structures of the gB ectodomain of several herpesviruses have been reported, the membrane fusion mechanism has remained elusive. Here, we report the X-ray structure of the Pseudorabies virus (PrV) gB ectodomain, revealing a typical class III post-fusion trimer that binds membranes via its fusion loops (FLs) in a cholesterol-dependent manner...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29045193/a-microdosimetric-kinetic-model-of-cell-killing-by-irradiation-from-permanently-incorporated-radionuclides
#18
Roland B Hawkins
An expression for the surviving fraction of a replicating population of cells exposed to permanently incorporated radionuclide is derived from the microdosimetric-kinetic model. It includes dependency on total implant dose, linear energy transfer (LET), decay rate of the radionuclide, the repair rate of potentially lethal lesions in DNA and the volume doubling time of the target population. This is used to obtain an expression for the biologically effective dose (BEDα/β) based on the minimum survival achieved by the implant that is equivalent to, and can be compared and combined with, the BEDα/β calculated for a fractionated course of radiation treatment...
October 18, 2017: Radiation Research
https://www.readbyqxmd.com/read/29029245/host-transcription-profile-in-nasal-epithelium-and-whole-blood-of-hospitalised-children-under-two-years-of-age-with-respiratory-syncytial-virus-infection
#19
Lien Anh Ha Do, Johann Pellet, H Rogier van Doorn, Anh Tuan Tran, Bach Hue Nguyen, Thi Thu Loan Tran, Quynh Huong Tran, Quoc Bao Vo, Nguyen Anh Tran Dac, Hong Nhien Trinh, Thi Thanh Hai Nguyen, Bao Tinh Le Binh, Huu Mai Khanh Nguyen, Minh Tien Nguyen, Quang Tung Thai, Thanh Vu Vo, Ngoc Quang Minh Ngo, Thi Kim Huyen Dang, Ngoc Huong Cao, Thu Van Tran, Lu Viet Ho, Bertrand De Meulder, Charles Auffray, Jorrit-Jan Hofstra, Jeremy Farrar, Juliet E Bryant, Menno de Jong, Martin L Hibberd
Most insights into the cascade of immune events following acute respiratory syncytial virus (RSV) infection have been obtained from animal experiments or in vitro models. Here we investigated host gene expression profiles in nasopharyngeal swabs (NP) and whole blood samples during natural RSV and rhinovirus (hRV) infection (acute versus early recovery phase) in 83 hospitalised patients <2 years old with lower respiratory tract infections (LRTI). RSV infection induced strong and persistent innate immune responses including interferon signaling and pathways related to chemokine/cytokine signaling in both compartments...
September 27, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29028430/curcumin-prevents-osteoarthritis-by-inhibiting-the-activation-of-inflammasome-nlrp3
#20
Yufeng Sun, Wei Liu, Hao Zhang, Hongtao Li, Jiakun Liu, Fayao Zhang, Tao Jiang, Shan Jiang
Curcumin has shown protective potential on osteoarthritis. However, its effect on treatment of osteoarthritis remains elusive so far. This study aimed to determine whether curcumin could ameliorate osteoarthritis in vivo and the underline mechanisms. The mice subjected to destabilization of the medial meniscus (DMM) surgery were administered curcumin. Cartilage integrity was evaluated by immunohistological staining. Expression levels of inflammatory cytokines from mice arthrodial cartilage were detected. THP-1 cells were primed by lipopolysaccharide (LPS)/ATP to induce inflammation, followed by the addition of curcumin...
October 2017: Journal of Interferon & Cytokine Research
keyword
keyword
106145
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"