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https://www.readbyqxmd.com/read/28928819/curcumin-induced-autophagy-anticancer-effects-on-human-lung-adenocarcinoma-cell-line-a549
#1
Furong Liu, Song Gao, Yuxuan Yang, Xiaodan Zhao, Yameng Fan, Wenxia Ma, Danrong Yang, Aimin Yang, Yan Yu
To investigate the anticancer effects of curcumin-induced autophagy and its effects on the human lung adenocarcinoma A549 cell line, inverted phase contrast microscopy was used to observe alterations to the cytomorphology of cells. An MTT assay was used to measure cell viability. Autophagy was detected using acridine orange (AO) staining and 3-methyladenine (3-MA) was used as an autophagy-specific inhibitor. Dose- and time-dependent A549 cell viability inhibition was observed following curcumin treatment. A dose-dependent increase in the red fluorescent structures in A549 cells was identified following curcumin treatment for 48 h through AO staining...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28918600/morphology-and-size-of-blood-cells-of-rhinella-arenarum-hensel-1867-as-environmental-health-assessment-in-disturbed-aquatic-ecosystem-from-central-argentina
#2
Zulma Anahí Salinas, Mariana Baraquet, Pablo Raúl Grenat, Adolfo Ludovico Martino, Nancy Edith Salas
Four populations of Rhinella arenarum from aquatic environments with different degrees of disturbance in central Argentina were compared to assess the ability of cytomorphology and cytomorphometry of blood cells as a hematological biomarker. A total of 93 specimens of R. arenarum (adults sexually mature) were captured during the spring. From the analysis of cell, no variations were found in terms of morphology, whereas in nuclear and cell areas and Price-Jones curves, we observed a smaller size in erythrocytes of individuals inhabiting the site most altered, "Villa Dalcar," as well as for leukocytes, lymphocytes, neutrophils, and eosinophils for the same site...
September 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28914981/fna-cytology-of-solitary-fibrous-tumors-and-the-diagnostic-value-of-stat6-immunocytochemistry
#3
Edneia Tani, Johan Wejde, Kristina Åström, Inga-Lill Wingmo, Olle Larsson, Felix Haglund
BACKGROUND: Solitary fibrous tumors (SFTs) are rare mesenchymal tumors commonly located in the pleura, soft tissues, or meninges and are characterized by the NGFI-A-binding protein 2 (NAB2)-signal transducer and activator of transcription 6 (STAT6) fusion gene. Recent studies have indicated that nuclear STAT6 immunohistochemistry is a specific marker for SFTs. METHODS: The authors reviewed fine-needle aspiration (FNA) specimens from extracranial SFTs diagnosed at their institution between 1993 and 2017...
September 15, 2017: Cancer
https://www.readbyqxmd.com/read/28913933/diagnostic-accuracy-of-fnac-and-cyto-histopathological-correlation-in-testicular-and-paratesticular-mass-lesions
#4
A D Singh, F A Wani, S Bhardwaj
OBJECTIVE: FNAC has a definitive role and has proved extremely useful in diagnosis of testicular and paratesticular mass lesions. In view of the dearth of literature of studies involving large cohorts of patients, the present study describes at length the detailed cytological evaluation of testicular and paratesticular mass lesions. METHODS: Our study consisted of 85 cases in 5-year retrospective and 1-year prospective analyses carried out in the Department of Pathology, Government Medical College, Jammu...
September 15, 2017: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/28902465/a-modified-reporting-approach-for-thyroid-fna-in-the-niftp-era-a-1-year-institutional-experience
#5
Jeffrey K Mito, Erik K Alexander, Trevor E Angell, Justine A Barletta, Matthew A Nehs, Edmund S Cibas, Jeffrey F Krane
BACKGROUND: The reclassification of noninvasive follicular variant of papillary thyroid carcinoma as noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) has created diagnostic and management issues for thyroid fine-needle aspiration (FNA). In response to these challenges, the authors' laboratory adopted a NIFTP policy including 1) stringent criteria (requiring pseudo-inclusions, papillae, and/or psammoma bodies) for a malignant diagnosis of papillary carcinoma to limit false-positive results due to NIFTP and 2) the use of explanatory notes in cases with cytomorphologic features suggestive of possible NIFTP to encourage lobectomy over thyroidectomy...
September 13, 2017: Cancer
https://www.readbyqxmd.com/read/28898989/role-of-histone-h3k27-trimethylation-loss-as-a-marker-for-malignant-peripheral-nerve-sheath-tumor-in-fine-needle-aspiration-and-small-biopsy-specimens
#6
Jeffrey K Mito, Xiaohua Qian, Leona A Doyle, Jason L Hornick, Vickie Y Jo
Objectives: Accurate diagnosis of malignant peripheral nerve sheath tumor (MPNST) is often challenging on fine-needle aspiration (FNA) or core needle biopsy. Recurrent mutations in EED and SUZ12, which encode subunits of polycomb repressive complex 2 (PRC2), have been identified in 70% to 92% of MPNSTs; PRC2 inactivation leads to loss of trimethylation of lysine 27 of histone H3 (H3K27me3). We evaluated the utility of H3K27me3 immunohistochemistry for distinguishing MPNST from its cytomorphologic mimics...
August 1, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28892908/cytological-diagnosis-of-an-uncommon-high-grade-malignant-thyroid-tumour-a-case-report
#7
Ruchi Nagpal, Manju Kaushal, Sawan Kumar
Anaplastic Thyroid Carcinoma (ATC) is a relatively uncommon highly malignant tumour originating from the follicular cells of thyroid gland having poor prognosis. It accounts for 2% to 5% of all thyroid carcinomas and patients typically present with a rapidly growing anterior neck mass with aggressive symptoms. A 53-year-old male presented with diffuse neck swelling measuring 8x6 cm and right cervical lymph node measuring 2x2 cm since one month which was associated with dyspepsia and dyspnoea. Ultrasound and Contrast Enhanced Computed Tomography (CECT) neck revealed enlarged right lobe of thyroid and multiple enlarged cervical lymph nodes with soft tissue density nodules in bilateral lungs...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28889392/isolation-and-characterization-of-ctcs-from-patients-with-cancer-of-a-urothelial-origin
#8
Vladimir Bobek, Katarina Kolostova
Monitoring of circulating tumor cells' (CTCs) presence has the potential to improve therapeutic management of oncological diseases at an early stage and also to identify patients with increased risk of tumor progression or recurrence before the onset of clinically detected metastasis. Here we describe a new simplified efficient methodology for the separation and in vitro culturing of viable CTCs from peripheral blood by size-based filtration (MetaCell(®)). The isolation protocol yields preferentially cells bigger than 8 μm enabling further cytomorphological and molecular analysis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28888064/the-cytopathology-of-actinomyces-nocardia-and-their-mimickers
#9
REVIEW
Kelsey E McHugh, Charles D Sturgis, Gary W Procop, Daniel D Rhoads
Nocardia species and Actinomyces species are 2 of the most commonly diagnosed filamentous bacteria in routine cytopathology practice. These genera share many overlapping cytomorphologic features, including their thin, beaded, branching, Gram-positive, GMS-positive filamentous structures that fragment at their peripheries into bacillary- and coccoid-appearing forms. Features that help distinguish between these 2 microorganisms include the width of their filamentous structures, the angles at which they branch, and their ability or lack thereof to retain a modified acid-fast stain...
September 9, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28885766/computer-assisted-cytologic-diagnosis-in-pancreatic-fna-an-application-of-neural-networks-to-image-analysis
#10
Amir Momeni-Boroujeni, Elham Yousefi, Jonathan Somma
BACKGROUND: Fine-needle aspiration (FNA) biopsy is an accurate method for the diagnosis of solid pancreatic masses. However, a significant number of cases still pose a diagnostic challenge. The authors have attempted to design a computer model to aid in the diagnosis of these biopsies. METHODS: Images were captured of cell clusters on ThinPrep slides from 75 pancreatic FNA cases (20 malignant, 24 benign, and 31 atypical). A K-means clustering algorithm was used to segment the cell clusters into separable regions of interest before extracting features similar to those used for cytomorphologic assessment...
September 8, 2017: Cancer
https://www.readbyqxmd.com/read/28881626/relevance-of-ccl3-ccr5-axis-in-oral-carcinogenesis
#11
Janine Mayra da Silva, Tálita Pollyanna Moreira Dos Santos, Lays Martin Sobral, Celso Martins Queiroz-Junior, Milene Alvarenga Rachid, Amanda E I Proudfoot, Gustavo Pompermaier Garlet, Aline Carvalho Batista, Mauro Martins Teixeira, Andréia Machado Leopoldino, Remo Castro Russo, Tarcília Aparecida Silva
The chemokine CCL3 is a chemotactic cytokine crucial for inflammatory cell recruitment in homeostatic and pathological conditions. CCL3 might stimulate cancer progression by promoting leukocyte accumulation, angiogenesis and tumour growth. The expression of CCL3 and its receptors CCR1 and CCR5 was demonstrated in oral squamous cell carcinoma (OSCC), but their role was not defined. Here, the functions of CCL3 were assessed using a model of chemically induced tongue carcinogenesis with 4-nitroquinoline-1-oxide (4NQO)...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28877067/diagnostic-and-prognostic-biomarkers-of-adrenal-cortical-carcinoma
#12
Ozgur Mete, Hasan Gucer, Mehmet Kefeli, Sylvia L Asa
The diagnosis of low-grade adrenal cortical carcinoma (ACC) confined to the adrenal gland can be challenging. Although there are diagnostic and prognostic molecular tests for ACC, they remain largely unutilized. We examined the diagnostic and prognostic value of altered reticulin framework and the immunoprofile of biomarkers including IGF-2, proteins involved in cell proliferation and mitotic spindle regulation (Ki67, p53, BUB1B, HURP, NEK2), DNA damage repair (PBK, γ-H2AX), telomere regulation (DAX, ATRX), wnt-signaling pathway (beta-catenin) and PI3K signaling pathway (PTEN, phospho-mTOR) in a tissue microarray of 50 adenomas and 43 carcinomas that were characterized for angioinvasion as defined by strict criteria, Weiss score, and mitotic rate-based tumor grade...
September 4, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28862206/lysosomal-storage-disorders-morphologic-appraisal-in-indian-population
#13
Dinesh Pradhan, Neelam Varma, Ashmita Gami, Kanwaljeet Singh Hura, Sambit K Mohanty
BACKGROUND: Lysosomal storage disorders (LSDs) comprise a group of at least 50 distinct genetic diseases, each one resulting from the deficiency of a particular lysosomal enzyme involved in metabolism. We attempt to study and further subclassify pediatric LSDs into Gaucher's and non-Gaucher's category based on the morphologic variables seen in the bone marrow aspiration smears and trephine biopsy sections. MATERIALS AND METHODS: Pediatric (<12 years age) cases of LSDs diagnosed by bone marrow aspiration and trephine biopsy specimens, in the last 12 years period, were retrieved...
July 2017: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28862049/pulmonary-adenocarcinoma-intra-alveolar-variant-a-rare-entity-mimicking-desquamative-interstitial-pneumonia
#14
Eric Hirsch, Jaishree Jagirdar, Alia Nazarullah
Exclusive discohesive tumor cells within intra-alveolar spaces is a rare pattern of involvement of primary and metastatic lung tumors. In the absence of a tumoral mass, this pattern closely mimics desquamative interstitial pneumonia (DIP) clinically, radiologically, and histologically. However, a secondary DIP pattern may be seen adjacent to a tumor mass not infrequently. Here, we describe a case of a 64-year-old woman status post bilateral lung transplantation, who was radiologically thought to have an interstitial lung disease...
August 1, 2017: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28844241/is-mobile-phone-radiation-genotoxic-an-analysis-of-micronucleus-frequency-in-exfoliated-buccal-cells
#15
F M de Oliveira, A M Carmona, C Ladeira
Electromagnetic fields (EMF) are classified as "possibly carcinogenic" by the International Agency for Research on Cancer (IARC). Some publications have reported associations between EMF exposure and DNA damage, but many other studies contradict such findings. Cytomorphological changes, such as micronuclei (MN), indicative of genomic damage, are biomarkers of genotoxicity. To test whether mobile phone-associated EMF exposure affects the MN frequency in exfoliated buccal cells, we obtained cells smears from the left and right inner cheeks of healthy mobile phone users, aged 18-30 (n=86), who also completed a characterization survey...
October 2017: Mutation Research
https://www.readbyqxmd.com/read/28839333/endometriosis-with-an-aberrant-immunophenotype-challenging-differential-diagnosis-of-glandular-lesions-in-the-pelvic-lymph-nodes
#16
Dita Vlckova, Jiri Lenz, Radek Chvatal, Jan Tihon, Miroslav Kavka, Lucie Uncapher
CASE REPORT: We describe an unusual case of pelvic lymph node endometriosis with an aberrant immunophenotype mimicking metastasis of adenocarcinoma. A 37-year-old patient with a history of invasive cervical adenocarcinoma stage pT1a2 is presented. Due to insufficient loop electrosurgical excision procedure (LEEP) conization, total laparoscopic hysterectomy with pelvic lymphadenectomy was indicated. Intraoperatively, the diagnosis of deep infiltrating endometriosis of parametrial ligament and vesicouterine pouch, endometrioma of the left ovary and Allen Master's syndrome was suspected; the patient had no history or clinical symptoms of endometriosis...
August 24, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/28835082/-fluorescence-in-situ-hybridization-combined-with-cytomorphology-for-the-detection-of-lung-cancer-in-bronchial-brushing-specimens
#17
S S Lu, Q J Pan, J Cao, X Xu, H Zhao, D H Shen
Objective: To evaluate the diagnostic value of fluorescence in situ hybridization (FISH) combined with bronchial brushing cytology for detecting lung cancer. Methods: Centromeric enumeration probes (CEPs) for chromosomes 7, 8 and 17 were used in FISH assay. The combination of FISH and cytology was analyzed in 69 bronchial brushing specimens. Results: The positive rates of CEP7, CEP8 and CEP17 in malignant cases diagnosed by cytology were 50.0%, 80.8% and 65.4%, respectively. CEP8 probe showed significantly higher positive rate than CEP7 (P=0...
August 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28834409/identification-of-distinct-cytomorphologic-features-in-the-diagnosis-of-niftp-at-the-time-of-preoperative-fna-implications-for-patient-management
#18
Jocelyn B Chandler, Monica Colunga, Manju L Prasad, Glenda G Callender, Courtney Quinn, David Chhieng, Adebowale J Adeniran
BACKGROUND: A major reclassification occurred with the redesignation of noninvasive encapsulated follicular variant of papillary thyroid carcinoma as noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) due to its indolent nature. The aim of this study was to determine whether distinct cytomorphologic features could be identified on preoperative fine-needle aspiration (FNA) when NIFTP cases were compared with invasive follicular variant of papillary thyroid carcinoma (FVPTC) subtypes...
August 21, 2017: Cancer
https://www.readbyqxmd.com/read/28834344/unusual-presentation-of-poorly-differentiated-primary-pulmonary-synovial-sarcoma-pd-ppss-diagnosed-by-ebus-tbna-with-cytogenetic-confirmation-a-diagnostic-challenge
#19
Sharvari Dalal, Charles E Nicholson, Darshana Jhala
Poorly differentiated primary pulmonary synovial sarcoma (PD-PPSS) is a rare, aggressive neoplasm, which occurs in 0.5% cases of all lung malignancies. The diagnosis of PD-PPSS can be very challenging on cytology samples. We present here an unusual case of PD-PPSS diagnosed by endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), in the setting of known history of diffuse large B-cell lymphoma. Diff-Quik and Papanicolaou stains showed cellular specimen with clusters of highly atypical small round blue cells admixed with lymphoid elements; and some with denuded cytoplasm...
August 18, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28808139/differential-expression-of-sirtuin-2-and-adipocyte-maturation-restriction-an-adaptation-process-during-hypoxia-in-fish
#20
Padmini Ekambaram, Parimala Parasuraman
Sirtuins have received widespread attention due to their diverse physiological role in metabolism. Among sirtuins, SIRT2 is more abundant in adipocytes and exerts effects on adipocyte differentiation, a process which involves conversion of preadipocytes to mature adipocytes orchestrated by adipokines and adipogenic transcription factors. Grey mullet (Mugil cephalus) was chosen as a study organism due to its excellent service as a biomonitor. Adipocytes isolated from natural field conditions were termed as field-hypoxic (Ennore) and -normoxic (Kovalam) based on dissolved oxygen (DO) level in the estuary...
September 15, 2017: Biology Open
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