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https://www.readbyqxmd.com/read/29053085/rifampicin-resistance-and-multidrug-resistant-tuberculosis-detection-using-xpert-mtb-rif-in-wuhan-china-a-retrospective-study
#1
Hai Huang, Yanlin Zhang, Sheng Li, Jun Wang, Jun Chen, Zhiyun Pan, Hui Gan
BACKGROUND: The Xpert MTB/RIF test (Cepheid, Sunnyvale, CA) can simultaneously detect the Mycobacterium tuberculosis (MTB) complex DNA and rifampicin (RFP) resistance and can rapidly determine RFP resistance and predict multidrug-resistant tuberculosis (MDR-TB). In this study, we analyzed clinical examination results of a hospital specializing in TB treatment in Wuhan, Hubei, China, and examined the use of traditional culture and drug-sensitive test (DST) results as a gold standard to assess the diagnosis value of the Xpert MTB/RIF test in RFP resistance and MDR-TB...
October 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28888636/efficacy-of-oral-bcg-vaccination-in-protecting-free-ranging-cattle-from-natural-infection-by-mycobacterium-bovis
#2
Graham Nugent, Ivor J Yockney, Jackie Whitford, Frank E Aldwell, Bryce M Buddle
Vaccination of cattle against bovine tuberculosis could be a valuable control strategy, particularly in countries faced with intractable ongoing infection from a disease reservoir in wildlife. A field vaccination trial was undertaken in New Zealand. The trial included 1286 effectively free-ranging cattle stocked at low densities in a remote 7600ha area, with 55% of them vaccinated using Mycobacterium bovis BCG (Danish strain 1311). Vaccine was administered orally in all but 34 cases (where it was injected)...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28751677/nontuberculous-mycobacteria-in-denmark-incidence-and-clinical-importance-during-the-last-quarter-century
#3
Thomas S Hermansen, Pernille Ravn, Erik Svensson, Troels Lillebaek
Disease caused by nontuberculous mycobacteria (NTM) is reported to increase due to an ageing population and a rise in the proportion of immunosuppressed patients. We did a retrospective cohort study of NTM-disease in the Danish population through a quarter-century to determine the disease burden and trends in annual incidence rates. 524,119 clinical specimens were cultured for mycobacteria from 1991 through 2015 at the International Reference Laboratory of Mycobacteriology in Denmark. Among these, 8,227 NTM strains were identified from 3,462 patients and distributed according to microbiological disease criteria...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674714/genexpert-technology-a-breakthrough-for-the-diagnosis-of-tuberculous-pericarditis-and-pleuritis-in-less-than-2-hours
#4
Muhammad Saeed, Mobeen Ahmad, Shagufta Iram, Saba Riaz, Mehwish Akhtar, Maleeha Aslam
OBJECTIVES: To evaluate the diagnostic validity of GeneXpert for the detection of Mycobacterium tuberculosis (MTB) in pericardial and pleural effusions samples. METHODS: A cross sectional study was conducted at the Mycobacteriology Laboratory, Allama Iqbal Medical College, Lahore, Pakistan. A total of 286 (158 pleural and 128 pericardial fluids) samples were received from tuberculosis (TB) suspects between January 2014 and August 2016. Every sample was processed for Ziehl-Neelsen (Zn) smear, Lowenstein Jensen (LJ) culture, GeneXpert MTB/RIF assay according to standard protocols...
July 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28666374/genomic-epidemiology-of-a-major-mycobacterium-tuberculosis-outbreak-retrospective-cohort-study-in-a-low-incidence-setting-using-sparse-time-series-sampling
#5
Dorte Bek Folkvardsen, Anders Norman, Åse Bengård Andersen, Erik Michael Rasmussen, Lars Jelsbak, Troels Lillebaek
Since 1992, Denmark has documented the largest outbreak of tuberculosis in Scandinavia ascribed to a single genotype, termed C2/1112-15. As of spring 2017, the International Reference Laboratory of Mycobacteriology in Copenhagen has collected and identified isolates from more than a thousand cases belonging to this outbreak via routine mycobacterial interspersed repetitive units-variable number of tandem repeats typing. Here, we present a retrospective analysis of the C2/1112-15 dataset, based on whole-genome data from a sparse time series consisting of 5 randomly selected isolates from 23 years of sampling...
August 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28566080/-molecular-epidemiology-of-human-mycobacterium-bovis-infection-in-aegean-region-turkey
#6
Cengiz Çavuşoğlu, Fethiye Ferda Yılmaz
Mycobacterium bovis, which has a broader host spectrum as opposed to Mycobacterium tuberculosis that generally causes disease in humans, mainly leads to chronic progressive pulmonary disease in a large number of domestic and wild mammals, particularly cattle. The term "zoonotic tuberculosis (TB)" is used to describe M.bovis infection in humans. "Zoonotic TB" can not be differentiated clinically, radiologically or pathologically from TB caused by M.tuberculosis. The aim of this study was to evaluate the role of M...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28549036/the-role-of-molecular-techniques-for-the-detection-of-mycobacterium-tuberculosis-complex-in-paraffin-embedded-biopsies
#7
Zaira Moure, Josep Castellví, Adrián Sánchez-Montalvá, Tomás Pumarola, M Teresa Tórtola
INTRODUCTION: Extrapulmonary tuberculosis (EPTB) is increasingly frequent in developed countries. When it is not clinically suspected, samples are not collected for culture and the only material available is a tissue paraffin block. OBJECTIVE: The aim of this study was to evaluate FluoroType MTB (FT-MTB) and GenoType MTBDRplus methods for the detection of Mycobaterium tuberculosis complex in paraffin-embedded biopsies comparing the results to tuberculosis diagnosis...
May 25, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28522316/mycobacteriological-characteristics-and-treatment-outcomes-in-extrapulmonary-mycobacterium-abscessus-complex-infections
#8
Suk Hyeon Jeong, Su-Young Kim, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee, Cheol-In Kang, Doo Ryeon Chung, Kyong Ran Peck, Sung Jae Shin, Won-Jung Koh
OBJECTIVES: The differentiation between Mycobacterium abscessus subspecies abscessus (M. abscessus) and Mycobacterium abscessus subspecies massiliense (M. massiliense) and determination of the presence of inducible resistance to macrolide antibiotics are important factors in the management of patients with Mycobacterium abscessus complex (MABC) infections. Unlike pulmonary MABC infections, little information on extrapulmonary MABC infections is available. METHODS: The molecular identification of clinical isolates was performed, and the clinical characteristics and treatment outcomes of 20 consecutive patients with extrapulmonary MABC infections were assessed...
July 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28087944/laboratory-diagnosis-and-susceptibility-testing-for-mycobacterium-tuberculosis
#9
REVIEW
Gary W Procop
The laboratory, which utilizes some of the most sophisticated and rapidly changing technologies, plays a critical role in the diagnosis of tuberculosis. Some of these tools are being employed in resource-challenged countries for the rapid detection and characterization of Mycobacterium tuberculosis. Foremost, the laboratory defines appropriate specimen criteria for optimal test performance. The direct detection of mycobacteria in the clinical specimen, predominantly done by acid-fast staining, may eventually be replaced by rapid-cycle PCR...
December 2016: Microbiology Spectrum
https://www.readbyqxmd.com/read/28057604/biological-markers-in-the-diagnosis-of-tuberculous-pleural-effusion
#10
REVIEW
Bastien Mollo, Stéphane Jouveshomme, François Philippart, Benoît Pilmis
Tuberculosis is one of the main etiologies to evoke in the context of lymphocyte pleurisy. However, diagnosis is difficult and is based on mycobacteriology that is not enough sensitive and time-consuming, or on histology that requires invasive biopsy gesture. This literature review, carried out from Medline, summarizes the main meta-analyzes, reviews, and originator publications in English on biomarkers, classic and more innovative, studied for the diagnosis of tuberculous pleurisy. Among the immuno-biochemical markers, interferon-γ (IFN-γ), isoenzyme of adenosine deaminase 2 (ADA2) and total adenosine deaminase (ADA) seem the most relevant with respective sensitivities of 89% (87-91), 97...
February 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28043494/yield-of-mycobacteriological-study-in-diagnosis-of-pleural-tuberculosis-among-human-immune-deficiency-virus-infected-patients
#11
Majid Marjani, Amir Yousefzadeh, Payam Tabarsi, Afshin Moniri, Ali Akbar Velayati
BACKGROUND: Pleural tuberculosis (TB) is common among HIV-infected patients. In the absence of HIV infection, the yield of mycobacteriological study is low and usually invasive procedures, including pleural fluid analysis and pleural biopsy, are necessary. The present study aimed to determine the yield of mycobacteriological study of sputum and pleural fluid among HIV-infected patients. METHODS: This retrospective case-control study involved HIV-positive and HIV-negative patients with new pleural TB admitted to the National Research Institute of Tuberculosis and Lung Disease, Tehran, Iran, for 5years...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28003422/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-combined-species-identification-and-drug-sensitivity-testing-in-mycobacteria
#12
Pieter-Jan Ceyssens, Karine Soetaert, Markus Timke, An Van den Bossche, Katrin Sparbier, Koen De Cremer, Markus Kostrzewa, Marijke Hendrickx, Vanessa Mathys
Species identification and drug susceptibility testing (DST) of mycobacteria are important yet complex processes traditionally reserved for reference laboratories. Recent technical improvements in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has started to facilitate routine mycobacterial identifications in clinical laboratories. In this paper, we investigate the possibility of performing phenotypic MALDI-based DST in mycobacteriology using the recently described MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA)...
February 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27990278/understanding-nontuberculous-mycobacterial-lung-disease-it-s-been-a-long-time-coming
#13
REVIEW
David E Griffith, Timothy R Aksamit
With a surprising predictability, most studies and reviews addressing therapy for nontuberculous mycobacterial (NTM) lung disease either start or end by mentioning the paucity of data from randomized and controlled trials. That is a legitimate criticism for NTM lung disease therapy, but it also somehow seems to influence attitudes toward all aspects of NTM investigation. Certainly the study of NTM diseases in general and NTM lung disease in particular is a recent development. Previously, NTM were viewed as minor, if inconvenient, pathogens similar to Mycobacterium tuberculosis...
2016: F1000Research
https://www.readbyqxmd.com/read/27804902/non-tuberculous-mycobacteria-epidemiological-pattern-in-a-reference-laboratory-and-risk-factors-associated-with-pulmonary-disease
#14
J Mencarini, C Cresci, M T Simonetti, C Truppa, G Camiciottoli, M L Frilli, P G Rogasi, S Veloci, M Pistolesi, G M Rossolini, A Bartoloni, F Bartalesi
The diseases caused by non-tuberculous mycobacteria (NTM), in both AIDS and non-AIDS populations, are increasingly recognized worldwide. Although the American Thoracic Society published the guidelines for diagnosis of NTM pulmonary disease (NTM-PD), the diagnosis is still difficult. In the first part of the study, we collected data on NTM isolates in the Mycobacteriology Laboratory of Careggi Hospital (Florence, Italy) and analysed the epidemiological data of NTM isolates. Then, to analyse the risk factors associated to NTM-PD, we studied the presence of ATS/IDSA criteria for NTM-PD in patients who had at least one positive respiratory sample for NTM and were admitted to the Infectious Disease Unit and the Section of Respiratory Medicine...
February 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/27733962/evaluation-of-culture-media-for-isolation-of-mycobacterium-species-from-human-clinical-specimens
#15
Padmavali Palange, Rahul Narang, Venkataramana Kandi
BACKGROUND: Laboratory diagnosis of tuberculosis has undergone a rapid change during last few years and a number of techniques for culture as well as molecular diagnosis have been used with their respective advantages and disadvantages. Sporadic studies have also reported the isolation of M. tuberculosis on standard blood agar (BA), which at one time was not considered as a suitable medium for mycobacterial culture. The present study was conducted to evaluate the routine use of 5% sheep BA in a mycobacteriology laboratory by comparing isolation rates and time for isolation of mycobacteria from pulmonary and extrapulmonary samples with those on Lowenstein-Jensen (LJ) medium...
August 30, 2016: Curēus
https://www.readbyqxmd.com/read/27730797/pioneers-in-medical-mycology-and-mycobacteriology
#16
Preeti Pillai, Mahesh Lakhe, Anjali Shetty, Rajeev Soman
No abstract text is available yet for this article.
February 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27525395/-investigation-of-mycobacterium-bovis-subsp-bovis-among-the-strains-of-mycobacterium-tuberculosis-complex-isolated-in-d%C3%A3-zce-province-turkey
#17
Cihadiye Elif Öztürk, İdris Şahin, Şükrü Öksüz, Nida Kılıç, Özge Kılınçel, Leyla Aydın, Dursun Atik, Emine Afşin
Throughout the history of mankind, tuberculosis (TB) has caused serious illness and still continues to do so. Archaeobiological studies indicated that TB in humans dates back to 4000-8000 BC, and cases were shown to be due to Mycobacterium bovis subsp.bovis rather than Mycobacterium tuberculosis. Moreover, this situation was thought to begin with domestication of animals, consumption of their milk, and living together in the same environment with them. Over time, with the consumption of boiled milk and with the establishment of separate animal shelters, M...
July 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27389290/-application-of-mass-spectrometry-in-mycobacteria
#18
Fernando Alcaide, Begoña Palop-Borrás, Diego Domingo, Griselda Tudó
To date, more than 170 species of mycobacteria have been described, of which more than one third may be pathogenic to humans, representing a significant workload for microbiology laboratories. These species must be identified in clinical practice, which has long been a major problem due to the shortcomings of conventional (phenotypic) methods and the limitations and complexity of modern methods largely based on molecular biology techniques. The aim of this review was to briefly describe different aspects related to the use of MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry (MS) for the identification of mycobacteria...
June 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27333026/diagnostic-molecular-mycobacteriology-in-regions-with-low-tuberculosis-endemicity-combining-real-time-pcr-assays-for-detection-of-multiple-mycobacterial-pathogens-with-line-probe-assays-for-identification-of-resistance-mutations
#19
Vanessa Deggim-Messmer, Guido V Bloemberg, Claudia Ritter, Antje Voit, Rico Hömke, Peter M Keller, Erik C Böttger
Molecular assays have not yet been able to replace time-consuming culture-based methods in clinical mycobacteriology. Using 6875 clinical samples and a study period of 35months we evaluated the use of PCR-based assays to establish a diagnostic workflow with a fast time-to-result of 1-2days, for 1. detection of Mycobacterium tuberculosis complex (MTB), 2. detection and identification of nontuberculous mycobacteria (NTM), and 3. identification of drug susceptible MTB. MTB molecular-based detection and culture gave concordant results for 97...
July 2016: EBioMedicine
https://www.readbyqxmd.com/read/27256154/active-tuberculosis-in-psoriasis-patients-treated-with-tnf-antagonists-a-french-nationwide-retrospective-study
#20
E Guinard, C Bulai Livideanu, H Barthélémy, M Viguier, Z Reguiai, M A Richard, D Jullien, N Beneton, C Bara, P Vabres, A Grandvuillemin, M C Marguery, F Amelot, M P Konstantinou, H Bagheri, C Paul
BACKGROUND: There is limited information about active tuberculosis (TB) occurring in psoriasis patients treated with Tumor necrosis factor (TNF) antagonists. OBJECTIVE: To describe the clinical characteristics of TB in psoriasis patients treated with TNF antagonists. METHODS: Nationwide retrospective study of psoriasis patients having experienced TB. Cases of TB were collected via three methods: search in the national pharmacosurveillance database, questionnaire to members of the French psoriasis research group, the college of French dermatology professors...
August 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
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