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https://www.readbyqxmd.com/read/29053877/trpv4-regulates-vascular-endothelial-permeability-during-colonic-inflammation-in-dextran-sulphate-sodium-induced-murine-colitis
#1
Kenjiro Matsumoto, Riho Yamaba, Ken Inoue, Daichi Utsumi, Takuya Tsukahara, Kikuko Amagase, Makoto Tominaga, Shinichi Kato
BACKGROUND AND PURPOSE: The transient receptor potential vanilloid 4 (TRPV4) is a nonselective cation channel involved in physical sensing in various tissue types. The present study aimed to elucidate the function and expression of TRPV4 in colonic vascular endothelial cells during dextran sulphate sodium (DSS)-induced colitis. EXPERIMENTAL APPROACH: The role of TRPV4 in the progression of colonic inflammation was examined in the 2 % DSS-induced murine colitis model using immunohistochemical analysis, western blotting, and Evans blue dye extrusion assay...
October 20, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29053791/pharmacological-targeting-of-apelin-impairs-glioblastoma-growth
#2
Elizabeth Harford-Wright, Gwennan Andre-Gregoire, Kathryn A Jacobs, Lucas Treps, Sophie Le Gonidec, Heloise M Leclair, Sara Gonzalez-Diest, Quentin Roux, François Guillonneau, Delphine Loussouarn, Lisa Oliver, François M Vallette, Fabienne Foufelle, Philippe Valet, Anthony P Davenport, Robert C Glen, Nicolas Bidere, Julie Gavard
Glioblastoma are highly aggressive brain tumours that are associated with an extremely poor prognosis. Within these tumours exists a subpopulation of highly plastic self-renewing cancer cells that retain the ability to expand ex vivo as tumourspheres, induce tumour growth in mice, and have been implicated in radio- and chemo-resistance. Although their identity and fate are regulated by external cues emanating from endothelial cells, the nature of such signals remains unknown. Here, we used a mass spectrometry proteomic approach to characterize the factors released by brain endothelial cells...
October 3, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29053761/the-transcription-factor-prdm16-marks-a-single-retinal-ganglion-cell-subtype-in-the-mouse-retina
#3
Sergio Groman-Lupa, Joseph Adewumi, Ko Uoon Park, Joseph A Brzezinski Iv
Purpose: Retinal ganglion cells (RGC) can be categorized into roughly 30 distinct subtypes. How these subtypes develop is poorly understood, in part because few unique subtype markers have been characterized. We tested whether the Prdm16 transcription factor is expressed by RGCs as a class or within particular ganglion cell subtypes. Methods: Embryonic and mature retinal sections and flatmount preparations were examined by immunohistochemistry for Prdm16 and several other cell type-specific markers...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29053606/lung-toxicity-of-condensed-aerosol-from-e-cig-liquids-influence-of-the-flavor-and-the-in-vitro-model-used
#4
Rossella Bengalli, Emanuele Ferri, Massimo Labra, Paride Mantecca
The diffusion of e-cigarette (e-CIG) opens a great scientific and regulatory debate about its safety. The huge number of commercialized devices, e-liquids with almost infinite chemical formulations and the growing market demand for a rapid and efficient toxicity screen system that is able to test all of these references and related aerosols. A consensus on the best protocols for the e-CIG safety assessment is still far to be achieved, since the huge number of variables characterizing these products (e.g., flavoring type and concentration, nicotine concentration, type of the device, including the battery and the atomizer)...
October 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29053592/platelets-and-multi-organ-failure-in-sepsis
#5
REVIEW
Elisabetta Greco, Enrico Lupia, Ornella Bosco, Barbara Vizio, Giuseppe Montrucchio
Platelets have received increasing attention for their role in the pathophysiology of infectious disease, inflammation, and immunity. In sepsis, a low platelet count is a well-known biomarker for disease severity and more recently authors have focused their attention on the active role of platelets in the pathogenesis of multi-organ failure. Septic shock is characterised by a dysregulated inflammatory response, which can impair the microcirculation and lead to organ injury. Being at the crossroads between the immune system, clotting cascade, and endothelial cells, platelets seem to be an appealing central mediator and possible therapeutic target in sepsis...
October 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29053562/predictive-factors-for-corneal-clearance-after-descemetorhexis-without-endothelial-keratoplasty
#6
Emma Davies, Ula Jurkunas, Roberto Pineda
PURPOSE: To study preoperative factors that possibly contribute to corneal clearance after Descemetorhexis without endothelial keratoplasty (DWEK) and to determine the most successful surgical technique for the procedure. METHODS: Retrospective chart review of 17 eyes of 13 patients with Fuchs corneal endothelial dystrophy that underwent central 4-mm DWEK. RESULTS: Baseline age, pachymetry, and endothelial cell count were not statistically significantly different between eyes that cleared 0 to 2 months, 3 to 5 months, 6 to 8 months, or that failed to clear...
October 17, 2017: Cornea
https://www.readbyqxmd.com/read/29053257/orthogonal-screening-of-anti-cancer-drugs-using-an-open-access-microfluidic-tissue-array-system
#7
Dongguo Lin, Peiwen Li, Jinqiong Lin, Bowen Shu, Weixin Wang, Qiong Zhang, Na Yang, Dayu Liu, Banglao Xu
Screening for potential drug combinations presents significant challenges to the current microfluidic cell culture systems, due to the requirement of flexibility in liquid handling. To overcome this limitation, we present here an open-access microfluidic tissue array system specifically designed for drug combination screening. The microfluidic chip features a key structure in which a nanoporous membrane is sandwiched by a cell culture chamber array layer and a corresponded media reservoir array layer. The microfluidic approach takes advantage of the characteristics of the nanoporous membrane: on one side, this membrane permits the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution; on the other side, it allows diffusion-based media exchange and thus, mimics the endothelial layer...
October 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29052766/decreased-diabetes-risk-over-9%C3%A2-year-after-18-month-oral-l-arginine-treatment-in-middle-aged-subjects-with-impaired-glucose-tolerance-and-metabolic-syndrome-extension-evaluation-of-l-arginine-study
#8
Lucilla D Monti, Elena Galluccio, Valentina Villa, Barbara Fontana, Serena Spadoni, Pier Marco Piatti
PURPOSE: This study aimed to determine whether L-arginine supplementation lasting for 18 months maintained long-lasting effects on diabetes incidence, insulin secretion and sensitivity, oxidative stress, and endothelial function during 108 months among subjects at high risk of developing type 2 diabetes. METHODS: One hundred and forty-four middle-aged subjects with impaired glucose tolerance and metabolic syndrome were randomized in 2006 to an L-arginine supplementation (6...
October 20, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/29052569/analysis-of-the-expression-of-angioarchitecture-related-factors-in-patients-with-cerebral-arteriovenous-malformation
#9
Guang-Zhong Chen, Yu Ke, Kun Qin, Meng-Qi Dong, Shao-Jian Zeng, Xiao-Feng Lin, Sheng-Quan Zhan, Kai Tang, Chao Peng, Xiao-Wen Ding, Dong Zhou
BACKGROUND: Cerebral arteriovenous malformation (cAVM) is a type of vascular malformation associated with vascular remodeling, hemodynamic imbalance, and inflammation. We detected four angioarchitecture-related cytokines to make a better understanding of the potential aberrant signaling in the pathogenesis of cAVM and found useful proteins in predicting the risk of cerebral hemorrhage. METHODS: Immunohistochemical analysis was conducted on specimens from twenty patients with cAVM diagnosed via magnetic resonance imaging and digital subtraction angiography and twenty primary epilepsy controls using antibodies against vascular endothelial growth factor receptor-2 (VEGFR-2), matrix metalloproteinase-9 (MMP-9), vascular cell adhesion molecule (VCAM-1), and endothelial nitric oxide synthase (eNOS)...
October 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/29052507/metabolic-shift-in-density-dependent-stem-cell-differentiation
#10
Simar J Singh, William Turner, Drew E Glaser, Kara E McCloskey, Fabian V Filipp
BACKGROUND: Vascular progenitor cells (VPCs) derived from embryonic stem cells (ESCs) are a valuable source for cell- and tissue-based therapeutic strategies. During the optimization of endothelial cell (EC) inductions from mouse ESCs using our staged and chemically-defined induction methods, we found that cell seeding density but not VEGF treatment between 10 ng/mL and 40 ng/mL was a significant variable directing ESCs into FLK1(+) VPCs during stage 1 induction. Here, we examine potential contributions from cell-to-cell signaling or cellular metabolism in the production of VPCs from ESCs seeded at different cell densities...
October 20, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29052437/5-6-dehydrokawain-from-the-rhizome-of-alpinia-mutica-roxb-induced-proangiogenic-tumour-derived-vegf-of-ht-29-colorectal-cancer
#11
Ibrahim Malami, Aliyu Muhammad, Ibrahim B Abubakar, Imaobong C Etti, Peter M Waziri, Ramadan M Abubakar, Halilu E Mshelia
Vascular endothelial growth factor (VEGF) is a glycoprotein vital to the regulation of vascular endothelial cells proliferation, migration and angiogenesis. The expression of VEGF is required for the formation of new blood vessels critical in supplying oxygen and nutrition in the course of tumorigenesis. The present study investigated the effect of 5,6-dehydrokawain isolated from the rhizomes of Alpinia mutica on VEGF expression in vitro using HT-29 cell line. The results revealed that 5,6-dehydrokawain induced the expression of proangiogenic tumour-derived VEGF of HT-29 cells, which may explain the inability of 5,6-dehydrokawain in suppressing cancer cells proliferation...
October 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29052398/-protective-effect-and-mechanism-of-scutellarin-ethyl-ester-on-focal-cerebral-ischemia-induced-by-ligation-of-middle-cerebral-artery-in-rats
#12
Xiao-Jing Ma, Lan Wang, Xiao-Jie Yin, Zhi-Min Wang, Xiao-Qian Liu, Lei-Lei Gong, Liang-Mian Chen, Ri-Xin Liang, Hui-Min Gao
To observe the protective effect of scutellarin ethyl ester on focal cerebral ischemia injury induced by middle cerebral artery occlusion in rats(MCAOR), and explore its mechanism. Totally 84 male SD rats were randomly divided into seven groups: sham-operated group, model group,positive drug group(niomdipine,12 mg•kg⁻¹), Brevisapin tablets group(48 mg•kg⁻¹), and high, middle and low-dose scutellarin ethyl ester groups(100, 50, 25 mg•kg⁻¹). The MCAOR model was prepared by using thread embolism method to observe the neurological function of rats, the area of cerebral infarction was measured with TTC, and the levels of MDA, SOD and NO in serum were detected with semiautomatic biochemistry analyzer...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052380/-action-mechanism-of-total-flavonoids-of-hippophae-rhamnoides-in-treatment-of-myocardial-ischemia-based-on-network-pharmacology
#13
Zhi-Cheng Wei, Dong Tong, Juan Yang, Ke-Hui Zhao, Xian-Li Meng, Yi Zhang
In this study, a network pharmacological screening method was adopted to further study the active ingredients and action mechanism of total flavonoids of Hippophae rhamnoides(TFH) for the treatment of myocardial ischemia. Firstly TCMSP database and PubChem database were searched, and then the data were combined with oral bioavailability and drug analysis to screen flavonoids of H.rhamnoides compounds. Then predictive analysis was conducted for the 7 screened compounds by ChemMapper server.The obtained potential targets were imported into MAS 3...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052367/decellularized-caprine-liver-extracellular-matrix-as-a-2d-substrate-coating-and-3d-hydrogel-platform-for-vascularized-liver-tissue-engineering
#14
Tarun Agarwal, Rajan Narayan, Somnath Maji, Sudip Kumar Ghosh, Tapas Kumar Maiti
Development of a vascularized liver tissue construct is a need of an hour to circumvent the current demand of liver transplantation in healthcare sector. An appropriate matrix must support liver cell viability, functionality, and development of microvasculature. With this perspective, here, we report the use of decellularized Caprine liver ECM (CLECM) derived hydrogel for tissue engineering applications. First, CLECM was used as a substrate coating material for 2D hepatocyte culture. HepG2 cells cultured on CLECM coated surface showed higher albumin, urea, glycogen and GAGs synthesis in comparison to collagen coated surface (taken as control for the study)...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29052177/prognostic-value-of-osteoprotegerin-and-srankl-in-bronchoalveolar-lavage-fluid-of-patients-with-advanced-non-small-cell-lung-cancer
#15
Wojciech Naumnik, I Płońska, M Ossolińska, J Nikliński, B Naumnik
Osteoprotegerin (OPG) is a soluble decoy receptor for receptor activator of nuclear factor-kappa B ligand (sRANKL). OPG promotes endothelial cell survival and neoangiogenesis. Dysregulation of the OPG/RANKL system has been detected in several tumors. In the present study, we evaluated the clinical usefulness of OPG and sRANKL assessment in bronchoalveolar lavage fluid (BALF) of patients with advanced non-small cell lung cancer (NSCLC). We measured the concentration of OPG and sRANKL in BALF of 44 NSCLC patients and 15 healthy volunteers taken as control subjects...
October 20, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29052078/carotid-artery-reactivity-during-sympathetic-activation-following-acute-resistance-exercise
#16
Kevin S Heffernan, Wesley K Lefferts, Eun Sun Yoon, Soo Hyun Park, Yong Hee Lee, Sae Young Jae
OBJECTIVE: Acute resistance exercise has been shown to reduce brachial endothelial function. Whether there are concomitant reductions in carotid endothelial function remains unexplored. METHODS: Cold pressor test-mediated vasodilation of the carotid artery was used to assess carotid endothelial function in 15 young and healthy participants (age 26 ± 1 years, body mass index 24 ± 1 kg/m(2)) after acute resistance exercise or an inactive time control condition...
October 19, 2017: Clinical Autonomic Research: Official Journal of the Clinical Autonomic Research Society
https://www.readbyqxmd.com/read/29051976/late-gadolinium-enhancement-on-cardiac-magnetic-resonance-imaging-is-associated-with-coronary-endothelial-dysfunction-in-patients-with-dilated-cardiomyopathy
#17
Mina Nakayama, Megumi Yamamuro, Seiji Takashio, Tomoaki Uemura, Naoki Nakayama, Kyoko Hirakawa, Seitaro Oda, Daisuke Utsunomiya, Koichi Kaikita, Seiji Hokimoto, Yasuyuki Yamashita, Yukiko Morita, Kazuo Kimura, Kouichi Tamura, Kenichi Tsujita
Myocardial fibrosis and coronary endothelial dysfunction are important determinants of outcome in patients with heart failure. However, the relationship of these factors in patients with dilated cardiomyopathy (DCM) is not fully understood. This study aimed to investigate the relationship between endothelium-dependent coronary vasomotor abnormality and late gadolinium enhancement (LGE) on cardiac magnetic resonance imaging (CMR) in patients with DCM. We examined 38 consecutive patients with DCM. All patients underwent CMR and the acetylcholine (ACh) provocation test using cardiac catheterization...
October 19, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/29051803/midostaurin-a-novel-therapeutic-agent-for-patients-with-flt3-mutated-acute-myeloid-leukemia-and-systemic-mastocytosis
#18
REVIEW
Molly M Gallogly, Hillard M Lazarus, Brenda W Cooper
The development of FLT3-targeted inhibitors represents an important paradigm shift in the management of patients with highly aggressive fms-like tyrosine kinase 3-mutated (FLT3-mut) acute myeloid leukemia (AML). Midostaurin is an orally administered type III tyrosine kinase inhibitor which in addition to FLT3 inhibits c-kit, platelet-derived growth factor receptors, src, and vascular endothelial growth factor receptor. Midostaurin is the first FLT3 inhibitor that has been shown to significantly improve survival in younger patients with FLT3-mut AML when given in combination with standard cytotoxic chemotherapy based on the recently completed RATIFY study...
September 2017: Therapeutic Advances in Hematology
https://www.readbyqxmd.com/read/29051760/interplay-of-gtpases-and-cytoskeleton-in-cellular-barrier-defects-during-gut-inflammation
#19
REVIEW
Rocío López-Posadas, Michael Stürzl, Imke Atreya, Markus F Neurath, Nathalie Britzen-Laurent
An essential role of the intestine is to build and maintain a barrier preventing the luminal gut microbiota from invading the host. This involves two coordinated physical and immunological barriers formed by single layers of intestinal epithelial and endothelial cells, which avoid the activation of local immune responses or the systemic dissemination of microbial agents, and preserve tissue homeostasis. Accordingly, alterations of epithelial and endothelial barrier functions have been associated with gut inflammation, for example during inflammatory bowel disease (IBD)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29051590/association-between-corneal-endothelial-cell-densities-and-elevated-cytokine-levels-in-the-aqueous-humor
#20
Yukari Yagi-Yaguchi, Takefumi Yamaguchi, Kazunari Higa, Terumasa Suzuki, Naohiko Aketa, Murat Dogru, Yoshiyuki Satake, Jun Shimazaki
Annual reduction rate of corneal endothelial cell density (ECD) varies among etiologies, however, the cause of chronic endothelial cell loss is still unknown. We recently reported the elevation of inflammatory cytokines in the aqueous humor (AqH) in eyes with bullous keratopathy and low ECD. To evaluate the association between ECD and aqueous cytokine levels, we collected a total of 157 AqH samples prospectively. The AqH levels of cytokines were measured and multivariate analyses were conducted to find the correlation between ECD, aqueous cytokine levels and clinical factors, such as number of previous intraocular surgeries and protein concentration in AqH...
October 19, 2017: Scientific Reports
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