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Sergi Clotet, Maria Jose Soler, Marta Riera, Julio Pascual, Fei Fang, Joyce Zhou, Ihor Batruch, Stella K Vasiliou, Apostolos Dimitromanolakis, Clara Barrios, Eleftherios Diamandis, James Scholey, Ana Konvalinka
Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17β-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated...
January 4, 2017: Molecular & Cellular Proteomics: MCP
Mark Roger Darling, Rebecca Woodford, Karl Kevin Cuddy, Linda Jackson-Boeters, Alyssa Hayter, Jeyanth Inkaran, Eleftherios P Diamandis, Zia Khan
AIM: The aim of the present study was to profile the expression of human kallikrein (KLK)-related peptidases (KLK) in odontogenic lesions. METHODS: Paraffin-embedded, formalin-fixed, non-odontogenic (control) and odontogenic lesions were stained for KLK using a standard immunohistochemical technique. The intensity and proportion of epithelial cells stained was scored. Reverse transcription-polymerase chain reaction was utilized to evaluate KLK 1-15 mRNA expression in ameloblastomas...
January 4, 2017: Journal of Investigative and Clinical Dentistry
Michelle Li, Eleftherios P Diamandis, David Grenache, Michael J Joyner, Daniel T Holmes, Rodger Seccombe
No abstract text is available yet for this article.
December 19, 2016: Clinical Chemistry
Vathany Kulasingam, Eleftherios P Diamandis
A blood test that can detect human malignancy with high clinical sensitivity and specificity is highly desirable. To achieve this, a tumor marker is needed that correlates with tumor burden and that can be measured with high analytical sensitivity and specificity. Over the past decades, a number of different types of tumor markers have emerged, including proteins such as enzymes, glycoproteins, and oncofetal antigens. Besides proteins, genetic abnormalities such as mutations, amplifications, and circulating tumor DNA have served as tumor markers...
November 17, 2016: BMC Medicine
Nicole White-Al Habeeb, Vathany Kulasingam, Eleftherios P Diamandis, George M Yousef, Gregory J Tsongalis, Louis Vermeulen, Ziqiang Zhu, Suzanne Kamel-Reid
No abstract text is available yet for this article.
December 2016: Clinical Chemistry
Carla M J Muytjens, Stella K Vasiliou, Katerina Oikonomopoulou, Ioannis Prassas, Eleftherios P Diamandis
Cervical-vaginal fluid (CVF) is a complex biological fluid that hydrates the mucosa of the lower female reproductive system. In-depth proteomic and biochemical studies on CVF have revealed that it contains large amounts of endogenous proteases and protease inhibitors, including an abundance of several members of the tissue kallikrein-related peptidase (KLK) family. Despite their ubiquitous presence in human tissues and fluids, KLK expression levels vary considerably, with maximum expression observed in reproduction-related tissues and fluids...
October 2016: Nature Reviews. Urology
Eleftherios P Diamandis, Mario Plebani
No abstract text is available yet for this article.
October 1, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Stella K Vasiliou, Eleftherios P Diamandis, George M Church, Henry T Greely, Françoise Baylis, Charis Thompson, Gerold Schmitt-Ulms
No abstract text is available yet for this article.
October 2016: Clinical Chemistry
Ana Konvalinka, Ihor Batruch, Tomas Tokar, Apostolos Dimitromanolakis, Shelby Reid, Xuewen Song, York Pei, Andrei P Drabovich, Eleftherios P Diamandis, Igor Jurisica, James W Scholey
BACKGROUND: Angiotensin-II (Ang II) mediates progression of autosomal-dominant polycystic kidney disease (ADPKD) and other chronic kidney diseases (CKD). However, markers of kidney Ang II activity are lacking. We previously defined 83 Ang II-regulated proteins in vitro, which reflected kidney Ang II activity in vivo. METHODS: In this study, we developed selected reaction monitoring (SRM) assays for quantification of Ang II-regulated proteins in urine of ADPKD and CKD patients...
2016: Clinical Proteomics
Tommaso A Dragani, Antoni Castells, Vathany Kulasingam, Eleftherios P Diamandis, Helena Earl, Wade T Iams, Christine M Lovly, J P Michiel Sedelaar, Jack A Schalken
Translational oncology represents a bridge between basic research and clinical practice in cancer medicine. Today, translational research in oncology benefits from an abundance of knowledge resulting from genome-scale studies regarding the molecular pathways involved in tumorigenesis. In this Forum article, we highlight the state of the art of translational oncology in five major cancer types. We illustrate the use of molecular profiling to subtype colorectal cancer for both diagnosis and treatment, and summarize the results of a nationwide screening program for ovarian cancer based on detection of a tumor biomarker in serum...
July 28, 2016: BMC Medicine
Felix Leung, Marcus Q Bernardini, Marshall D Brown, Yingye Zheng, Rafael Molina, Robert C Bast, Gerard Davis, Stefano Serra, Eleftherios P Diamandis, Vathany Kulasingam
BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy. Our integrated -omics approach to ovarian cancer biomarker discovery has identified kallikrein 6 (KLK6) and folate-receptor 1 (FOLR1) as promising candidates but these markers require further validation. METHODS: KLK6, FOLR1, CA125, and HE4 were investigated in three independent serum cohorts with a total of 20 healthy controls, 150 benign controls, and 216 ovarian cancer patients. The serum biomarker levels were determined by ELISA or automated immunoassay...
September 2016: Cancer Epidemiology, Biomarkers & Prevention
Phedias Diamandis, David Chitayat, Ants Toi, S Blaser, Patrick Shannon
OBJECTIVE: To characterise the early tissue changes of post encephaloclastic polymicrogyria in the human fetus. METHODS: We identified and reviewed the clinical histories and autopsy pathology of post ischemic fetal cerebral cortical injury at less than 30weeks gestational age (GA). The histology of local cortical abnormalities was examined with neuronal, glial, microglial and vascular immunohistochemical markers. RESULTS: We identified eight cases ranging from 18 to 29weeks GA: 5 cases show full thickness cortical infarcts and 3 show periSylvian post-ischemic necrosis of the cerebral cortex...
January 2017: Brain & Development
Ashley Di Meo, Ihor Batruch, Arsani G Yousef, Maria D Pasic, Eleftherios P Diamandis, George M Yousef
BACKGROUND: Urine represents an ideal source of clinically relevant biomarkers as it contains a large number of proteins and low molecular weight peptides. The comprehensive characterization of the normal urinary proteome and peptidome can serve as a reference for future biomarker discovery. Proteomic and peptidomic analysis of urine can also provide insight into normal physiology and disease pathology, especially for urogenital diseases. METHODS: We developed an integrated proteomic and peptidomic analytical protocol in normal urine...
February 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
Theano D Karakosta, Antoninus Soosaipillai, Eleftherios P Diamandis, Ihor Batruch, Andrei P Drabovich
Human kallikrein-related peptidases (KLKs) are a group of 15 secreted serine proteases encoded by the largest contiguous cluster of protease genes in the human genome. KLKs are involved in coordination of numerous physiological functions including regulation of blood pressure, neuronal plasticity, skin desquamation, and semen liquefaction, and thus represent promising diagnostic and therapeutic targets. Until now, quantification of KLKs in biological and clinical samples was accomplished by enzyme-linked immunosorbent assays (ELISA)...
September 2016: Molecular & Cellular Proteomics: MCP
Katerina Oikonomopoulou, Herbert Yu, Zhanwei Wang, Stella K Vasiliou, Davor Brinc, Georgios Christofi, Marilena Theodorou, Pavlos Pavlou, Andreas Hadjisavvas, Christiana A Demetriou, Kyriacos Kyriacou, Eleftherios P Diamandis
BACKGROUND: Infections from microorganisms and parasites have been connected with either increased or decreased cancer risk. The objective of this study was to investigate whether infection by Echinococcus granulosus is associated with cancer risk. METHODS: We assembled a pilot retrospective cohort of patients who were diagnosed as being infected by E. granulosus in Cyprus between 1930 and 2011. Age/gender-matched non-infected family members and neighbors were selected as references...
June 21, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Felix Leung, Vathany Kulasingam, Eleftherios P Diamandis, Dave S B Hoon, Kenneth Kinzler, Klaus Pantel, Catherine Alix-Panabières
No abstract text is available yet for this article.
August 2016: Clinical Chemistry
Natasha Musrap, Eleftherios P Diamandis
BACKGROUND: Since its discovery in the 1970s, prostate-specific antigen (PSA) has become widely known as a biomarker of prostate cancer in males but has often been overlooked in female malignancies. Although the serum concentration of PSA differs between men and women by about 1000-fold, studies have suggested that PSA concentrations drastically differ among healthy females and those who exhibit increased androgen production. CONTENT: There have been reports of increased PSA expression in women exhibiting hyperandrogenic states, including polycystic ovary syndrome and hirsutism, as well as marked increases in a subset of breast cancer patients...
August 2016: Clinical Chemistry
Eleftherios P Diamandis
No abstract text is available yet for this article.
August 1, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Ilijana Begcevic, Davor Brinc, Andrei P Drabovich, Ihor Batruch, Eleftherios P Diamandis
BACKGROUND: Cerebrospinal fluid (CSF) is a proximal fluid which communicates closely with brain tissue, contains numerous brain-derived proteins and thus represents a promising fluid for discovery of biomarkers of central nervous system (CNS) diseases. The main purpose of this study was to generate an extensive CSF proteome and define brain-related proteins identified in CSF, suitable for development of diagnostic assays. METHODS: Six non-pathological CSF samples from three female and three male individuals were selected for CSF analysis...
2016: Clinical Proteomics
Alain Mangé, Lampros Dimitrakopoulos, Antoninus Soosaipillai, Peter Coopman, Eleftherios P Diamandis, Jérôme Solassol
UNLABELLED: Secreted proteins constitute a relevant source of putative cancer biomarkers. Here, we compared the secretome of a series of four genetically-related breast cancer cell lines as a model of aggressiveness using quantitative mass spectrometry. 537 proteins (59.5% of the total identified proteins) predicted to be released or shed from cells were identified. Using a scoring system based on i) iTRAQ value, ii) breast cancer tissue mRNA expression levels, and iii) immunohistochemical staining (public database), a short list of 10 candidate proteins was selected...
June 16, 2016: Journal of Proteomics
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