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https://www.readbyqxmd.com/read/28330469/preclinical-evaluation-of-a-tex101-protein-elisa-test-for-the-differential-diagnosis-of-male-infertility
#1
Dimitrios Korbakis, Christina Schiza, Davor Brinc, Antoninus Soosaipillai, Theano D Karakosta, Christine Légaré, Robert Sullivan, Brendan Mullen, Keith Jarvi, Eleftherios P Diamandis, Andrei P Drabovich
BACKGROUND: TEX101 is a cell membrane protein exclusively expressed by testicular germ cells and shed into seminal plasma. We previously verified human TEX101 as a biomarker for the differential diagnosis of azoospermia, and developed a first-of-its-kind TEX101 ELISA. To demonstrate the clinical utility of TEX101, in this work we aimed at evaluating ELISA performance in a large population of fertile, subfertile, and infertile men. METHODS: Mass spectrometry, size-exclusion chromatography, ultracentrifugation, and immunohistochemistry were used to characterize TEX101 protein as an analyte in seminal plasma...
March 23, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28238811/the-current-peer-review-system-is-unsustainable-awaken-the-paid-reviewer-force
#2
Eleftherios P Diamandis
No abstract text is available yet for this article.
February 23, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28157690/variant-peptide-detection-utilizing-mass-spectrometry-laying-the-foundations-for-proteogenomic-identification-and-validation
#3
Lampros Dimitrakopoulos, Ioannis Prassas, Els M J J Berns, John A Foekens, Eleftherios P Diamandis, George S Charames
BACKGROUND: Proteogenomics is an emerging field at the intersection of genomics and proteomics. Many variant peptides corresponding to single nucleotide variations (SNVs) are associated with specific diseases. The aim of this study was to demonstrate the feasibility of proteogenomic-based variant peptide detection in disease models and clinical specimens. METHODS: We sought to detect p53 single amino acid variant (SAAV) peptides in breast cancer tumor samples that have been previously subjected to sequencing analysis...
February 3, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28115917/a-new-enzyme-linked-immunosorbent-assay-elisa-for-human-free-and-bound-kallikrein-9
#4
Panagiota Filippou, Dimitrios Korbakis, Sofia Farkona, Antoninus Soosaipillai, Theano Karakosta, Eleftherios P Diamandis
BACKGROUND: Kallikrein 9 (KLK9) is a member of the human kallikrein-related peptidases family, whose physiological role and implications in disease processes remain unclear. The active form of the enzyme is predicted to have chymotryptic activity. In the present study, we produced for the first time the active recombinant protein and monoclonal antibodies, and developed novel immunoassays for the quantification of free and bound KLK9 in biological samples. METHODS: The coding sequence of mature KLK9 isoform (mat-KLK9) was expressed in an Expi293F mammalian system and the synthesized polypeptide was purified through a two-step protocol...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28095327/proteomic-and-peptidomic-analysis-of-human-sweat-with-emphasis-on-proteolysis
#5
Yijing Yu, Ioannis Prassas, Carla M J Muytjens, Eleftherios P Diamandis
Sweat is produced by eccrine and apocrine glands and represents a biological fluid with established roles in thermo-regulation and host infection defense. The composition of sweat is highly dynamic and alters significantly in various skin and other disorders. Therefore, in-depth profiling of sweat protein composition is expected to augment our understanding of the pathobiology of several skin diseases and may lead to the identification of useful sweat-based disease biomarkers. We here reported an in-depth analysis of the human sweat proteome and peptidome...
January 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28088919/the-journal-impact-factor-is-under-attack-use-the-capci-factor-instead
#6
EDITORIAL
Eleftherios P Diamandis
The uses and misuses of the Journal Impact Factor (JIF) have been thoroughly discussed in the literature. A few years ago, I predicted that JIF would soon be replaced, while another colleague argued the opposite. Over the past few months, attacks on JIF have intensified, with some publishing organizations gradually removing the indicator from their journals' websites. Here, I argue that most, if not all of the misuses of JIF are related to its name. The word "impact" should be removed, since it implies an influential attribute, either for the journals, their published papers, or their authors...
January 16, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28062795/silac-based-proteomics-of-primary-human-kidney-cells-reveals-a-novel-link-between-male-sex-hormones-and-impaired-energy-metabolism-in-diabetic-kidney-disease
#7
Sergi Clotet, Maria Jose Soler, Marta Riera, Julio Pascual, Fei Fang, Joyce Zhou, Ihor Batruch, Stella K Vasiliou, Apostolos Dimitromanolakis, Clara Barrios, Eleftherios Diamandis, James Scholey, Ana Konvalinka
Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17β-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated...
January 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28054463/kallikrein-related-peptidase-expression-in-odontogenic-cysts-and-tumors-an-immunohistochemical-comparative-study
#8
Mark Roger Darling, Rebecca Woodford, Karl Kevin Cuddy, Linda Jackson-Boeters, Alyssa Hayter, Jeyanth Inkaran, Eleftherios P Diamandis, Zia Khan
AIM: The aim of the present study was to profile the expression of human kallikrein (KLK)-related peptidases (KLK) in odontogenic lesions. METHODS: Paraffin-embedded, formalin-fixed, non-odontogenic (control) and odontogenic lesions were stained for KLK using a standard immunohistochemical technique. The intensity and proportion of epithelial cells stained was scored. Reverse transcription-polymerase chain reaction was utilized to evaluate KLK 1-15 mRNA expression in ameloblastomas...
January 4, 2017: Journal of Investigative and Clinical Dentistry
https://www.readbyqxmd.com/read/27993938/direct-to-consumer-testing
#9
Michelle Li, Eleftherios P Diamandis, David Grenache, Michael J Joyner, Daniel T Holmes, Rodger Seccombe
No abstract text is available yet for this article.
December 19, 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27852253/genomic-profiling-for-copy-number-changes-in-plasma-of-ovarian-cancer-patients-a-new-era-for-cancer-diagnostics
#10
EDITORIAL
Vathany Kulasingam, Eleftherios P Diamandis
A blood test that can detect human malignancy with high clinical sensitivity and specificity is highly desirable. To achieve this, a tumor marker is needed that correlates with tumor burden and that can be measured with high analytical sensitivity and specificity. Over the past decades, a number of different types of tumor markers have emerged, including proteins such as enzymes, glycoproteins, and oncofetal antigens. Besides proteins, genetic abnormalities such as mutations, amplifications, and circulating tumor DNA have served as tumor markers...
November 17, 2016: BMC Medicine
https://www.readbyqxmd.com/read/27679436/the-use-of-targeted-therapies-for-precision-medicine-in-oncology
#11
Nicole White-Al Habeeb, Vathany Kulasingam, Eleftherios P Diamandis, George M Yousef, Gregory J Tsongalis, Louis Vermeulen, Ziqiang Zhu, Suzanne Kamel-Reid
No abstract text is available yet for this article.
December 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27603220/putative-functions-of-tissue-kallikrein-related-peptidases-in-vaginal-fluid
#12
REVIEW
Carla M J Muytjens, Stella K Vasiliou, Katerina Oikonomopoulou, Ioannis Prassas, Eleftherios P Diamandis
Cervical-vaginal fluid (CVF) is a complex biological fluid that hydrates the mucosa of the lower female reproductive system. In-depth proteomic and biochemical studies on CVF have revealed that it contains large amounts of endogenous proteases and protease inhibitors, including an abundance of several members of the tissue kallikrein-related peptidase (KLK) family. Despite their ubiquitous presence in human tissues and fluids, KLK expression levels vary considerably, with maximum expression observed in reproduction-related tissues and fluids...
October 2016: Nature Reviews. Urology
https://www.readbyqxmd.com/read/27574855/theranos-phenomenon-part-5-theranos-presentation-at-the-american-association-for-clinical-chemistry-annual-conference-2016
#13
Eleftherios P Diamandis, Mario Plebani
No abstract text is available yet for this article.
October 1, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27551006/crispr-cas9-system-opportunities-and-concerns
#14
Stella K Vasiliou, Eleftherios P Diamandis, George M Church, Henry T Greely, Françoise Baylis, Charis Thompson, Gerold Schmitt-Ulms
No abstract text is available yet for this article.
October 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27499720/quantification-of-angiotensin-ii-regulated-proteins-in-urine-of-patients-with-polycystic-and-other-chronic-kidney-diseases-by-selected-reaction-monitoring
#15
Ana Konvalinka, Ihor Batruch, Tomas Tokar, Apostolos Dimitromanolakis, Shelby Reid, Xuewen Song, York Pei, Andrei P Drabovich, Eleftherios P Diamandis, Igor Jurisica, James W Scholey
BACKGROUND: Angiotensin-II (Ang II) mediates progression of autosomal-dominant polycystic kidney disease (ADPKD) and other chronic kidney diseases (CKD). However, markers of kidney Ang II activity are lacking. We previously defined 83 Ang II-regulated proteins in vitro, which reflected kidney Ang II activity in vivo. METHODS: In this study, we developed selected reaction monitoring (SRM) assays for quantification of Ang II-regulated proteins in urine of ADPKD and CKD patients...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27469586/major-milestones-in-translational-oncology
#16
EDITORIAL
Tommaso A Dragani, Antoni Castells, Vathany Kulasingam, Eleftherios P Diamandis, Helena Earl, Wade T Iams, Christine M Lovly, J P Michiel Sedelaar, Jack A Schalken
Translational oncology represents a bridge between basic research and clinical practice in cancer medicine. Today, translational research in oncology benefits from an abundance of knowledge resulting from genome-scale studies regarding the molecular pathways involved in tumorigenesis. In this Forum article, we highlight the state of the art of translational oncology in five major cancer types. We illustrate the use of molecular profiling to subtype colorectal cancer for both diagnosis and treatment, and summarize the results of a nationwide screening program for ovarian cancer based on detection of a tumor biomarker in serum...
July 28, 2016: BMC Medicine
https://www.readbyqxmd.com/read/27448593/validation-of-a-novel-biomarker-panel-for-the-detection-of-ovarian-cancer
#17
Felix Leung, Marcus Q Bernardini, Marshall D Brown, Yingye Zheng, Rafael Molina, Robert C Bast, Gerard Davis, Stefano Serra, Eleftherios P Diamandis, Vathany Kulasingam
BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy. Our integrated -omics approach to ovarian cancer biomarker discovery has identified kallikrein 6 (KLK6) and folate-receptor 1 (FOLR1) as promising candidates but these markers require further validation. METHODS: KLK6, FOLR1, CA125, and HE4 were investigated in three independent serum cohorts with a total of 20 healthy controls, 150 benign controls, and 216 ovarian cancer patients. The serum biomarker levels were determined by ELISA or automated immunoassay...
September 2016: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/27406708/the-pathology-of-incipient-polymicrogyria
#18
Phedias Diamandis, David Chitayat, Ants Toi, S Blaser, Patrick Shannon
OBJECTIVE: To characterise the early tissue changes of post encephaloclastic polymicrogyria in the human fetus. METHODS: We identified and reviewed the clinical histories and autopsy pathology of post ischemic fetal cerebral cortical injury at less than 30weeks gestational age (GA). The histology of local cortical abnormalities was examined with neuronal, glial, microglial and vascular immunohistochemical markers. RESULTS: We identified eight cases ranging from 18 to 29weeks GA: 5 cases show full thickness cortical infarcts and 3 show periSylvian post-ischemic necrosis of the cerebral cortex...
January 2017: Brain & Development
https://www.readbyqxmd.com/read/27394047/an-integrated-proteomic-and-peptidomic-assessment-of-the-normal-human-urinome
#19
Ashley Di Meo, Ihor Batruch, Arsani G Yousef, Maria D Pasic, Eleftherios P Diamandis, George M Yousef
BACKGROUND: Urine represents an ideal source of clinically relevant biomarkers as it contains a large number of proteins and low molecular weight peptides. The comprehensive characterization of the normal urinary proteome and peptidome can serve as a reference for future biomarker discovery. Proteomic and peptidomic analysis of urine can also provide insight into normal physiology and disease pathology, especially for urogenital diseases. METHODS: We developed an integrated proteomic and peptidomic analytical protocol in normal urine...
February 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27371727/quantification-of-human-kallikrein-related-peptidases-in-biological-fluids-by-multiplatform-targeted-mass-spectrometry-assays
#20
Theano D Karakosta, Antoninus Soosaipillai, Eleftherios P Diamandis, Ihor Batruch, Andrei P Drabovich
Human kallikrein-related peptidases (KLKs) are a group of 15 secreted serine proteases encoded by the largest contiguous cluster of protease genes in the human genome. KLKs are involved in coordination of numerous physiological functions including regulation of blood pressure, neuronal plasticity, skin desquamation, and semen liquefaction, and thus represent promising diagnostic and therapeutic targets. Until now, quantification of KLKs in biological and clinical samples was accomplished by enzyme-linked immunosorbent assays (ELISA)...
September 2016: Molecular & Cellular Proteomics: MCP
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