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https://www.readbyqxmd.com/read/29145645/waddlia-chondrophila-a-chlamydia-related-bacterium-has-a-negative-impact-on-human-spermatozoa
#1
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29144829/complications-assoicated-with-miragel-for-treatment-of-retinal-detachment
#2
Miin Roh, Nahyoung Grace Lee, John B Miller
MIRAgel (MIRA, Waltham, MA) is a hydrogel buckle that was introduced in 1979 as a new scleral implant for the treatment of retinal detachment. Long-term follow-up of more than 10 years revealed that the hydrolysis of the synthetic hydrophilic material leads to marked expansion of the substance, causing complications such as buckle extrusion and intrusion, eye motility disorder, cosmetic deformities, and periocular infections. Removal of the implant is the treatment of choice in cases with complications, but it is technically difficult due to the friability of the implant, severe scleral ectasia, and relatively high rate of redetachment after removal...
November 16, 2017: Seminars in Ophthalmology
https://www.readbyqxmd.com/read/29144232/saccharopolyspora-aidingensis-sp-nov-an-actinomycete-isolated-from-a-salt-lake
#3
Zhan-Feng Xia, Xiao-Xia Luo, Chuan-Xing Wan, Li-Li Zhang
A novel halophilic actinomycete strain, designated TRM 46074(T), was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074(T) was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose...
March 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29144018/navigated-retrograde-endoscopic-myotomy-rem-for-the-treatment-of-therapy-resistant-achalasia
#4
K D Pham, R F Havre, T Langø, E F Hofstad, G A Tangen, R Mårvik, T Pham, O H Gilja, J G Hatlebakk, A Viste
BACKGROUND: In achalasia, muscle spasm may involve the proximal esophagus. When the muscle spasm is located in the proximal esophagus, conventional per oral endoscopic myotomy (POEM) may not be sufficient to relieve symptoms. In this paper, we describe retrograde endoscopic myotomy (REM) as a novel approach to perform myotomy of the proximal esophagus, with the application of a navigation tool for anatomical guidance during REM. We aim to evaluate the feasibility and safety of REM and usefulness of the navigation during REM...
November 16, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29143963/expression-of-paraoxonase-types-1-2-and-3-in-reproductive-tissues-and-activity-of-paraoxonase-type-1-in-the-serum-and-seminal-plasma-of-bulls
#5
C E R Ferreira, C S Haas, K L Goularte, M T Rovani, F F Cardoso, A Schneider, B G Gasperin, T Lucia
The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT-PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls...
November 16, 2017: Andrologia
https://www.readbyqxmd.com/read/29143943/the-impact-of-ejaculatory-abstinence-on-semen-analysis-parameters-a-systematic-review
#6
REVIEW
Brent M Hanson, Kenneth I Aston, Tim G Jenkins, Douglas T Carrell, James M Hotaling
OBJECTIVE: The aim of this study was to evaluate recent publications and determine the impact of ejaculatory abstinence on semen analysis parameters as well as fertility outcomes. METHODS: This was a systematic review of 28 recent publications. The focus of this study was the impact of abstinence on semen parameters and fertility outcomes in papers published since the year 2000. The specific parameters evaluated were volume, sperm count, motility, morphology, pH, DNA fragmentation rate, viability, and pregnancy or fertilization rates following assisted reproduction...
November 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29143891/in-search-of-the-ideal-promotility-agent-optimal-use-of-currently-available-promotility-agents-for-nutrition-therapy-of-the-critically-ill-patient
#7
REVIEW
Sarah J Diamond, Endashaw Omer, Laszlo Kiraly
PURPOSE OF REVIEW: Enteral nutrition therapy is essential in the management of critically ill patients. Prokinetic agents have been used successfully to aid in the delivery of nutrition and improve feeding tolerance in patients in the intensive care unit (ICU). The aim of this report is to review the existing promotility agents available for use in the critically ill as well as outline the role of potential investigative drugs in order to provide a guide to the management of this difficult and important clinical dilemma...
November 16, 2017: Current Gastroenterology Reports
https://www.readbyqxmd.com/read/29143799/effect-of-a-partially-hydrolysed-whey-infant-formula-supplemented-with-starch-and-lactobacillus-reuteri-dsm-17938-on-regurgitation-and-gastric-motility
#8
Flavia Indrio, Giuseppe Riezzo, Paola Giordano, Maria Ficarella, Maria Paola Miolla, Silvia Martini, Luigi Corvaglia, Ruggiero Francavilla
Functional regurgitation (FR) is common in early infancy and represents a major drain on healthcare resources. This double-blind, randomized controlled trial investigated the effects of a formula containing partially hydrolysed, 100% whey protein, starch and Lactobacillus reuteri (DSM 17938) on gastric emptying rate (GErate) and regurgitation frequency in infants with FR. Enrolled infants were randomly allocated to receive either the test formula or a standard starter formula for four weeks. Ultrasound GErate assessment was performed at baseline (week 0) and at week 4; the number of regurgitations, feed volumes and potential adverse events were recorded in a daily diary...
October 28, 2017: Nutrients
https://www.readbyqxmd.com/read/29143769/senescent-nephropathy-the-new-renal-syndrome
#9
REVIEW
Florencia Aiello, Eliana P Dueñas, Carlos G Musso
Chronic kidney disease (CKD) is a condition characterized by progressive and irreversible deterioration of renal function due to the reduction of nephron mass for a period of at least three months. The prevalence of CKD is roughly 10% in the general population but increases with age, affecting more than one-third of people older than 65. Frailty is a condition usually found in elderly people, characterized by weakness, motility, and balance issues, with a declined ability to resist stressors leading to increased risks of adverse health outcomes including falls, fracture, hospitalization, institutionalization, disability, dependence, dementia, poor quality of life, and death...
October 28, 2017: Healthcare (Basel, Switzerland)
https://www.readbyqxmd.com/read/29143729/thalassotalea-coralli-sp-nov-isolated-from-the-torch-coral-euphyllia-glabrescens
#10
Der-Shyan Sheu, Shih-Yi Sheu, Pei-Bei Xie, Sen-Lin Tang, Wen-Ming Chen
Strain Eup a-8(T), isolated from a torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-8(T) were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly-β-hydroxybutyrate-containing, rod-shaped and formed white colonies. Optimal growth occurred at 25-30 °C, pH 7-8, and in the presence of 2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-8(T) belonged to the genus Thalassotalea and showed the highest levels of sequence similarity with respect to Thalassotalea ganghwensis JC2041(T) (97...
November 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29143656/does-evaluation-of-in-vitro-microfilarial-motility-reflect-the-resistance-status-of-dirofilaria-immitis-isolates-to-macrocyclic-lactones
#11
Mary J Maclean, Molly D Savadelis, Ruby Coates, Michael T Dzimianski, Corey Jones, Cynthia Benbow, Bobby E Storey, Ray M Kaplan, Andrew R Moorhead, Adrian J Wolstenholme
BACKGROUND: Several reports have confirmed that macrocyclic lactone-resistant isolates of Dirofilaria immitis are circulating in the United States; however, the prevalence and potential impact of drug resistance is unknown. We wished to assess computer-aided measurements of motility as a method for rapidly assessing the resistance status of parasite isolates. METHODS: Blood containing microfilariae (MF) from two clinical cases with a high suspicion of resistance was fed to mosquitoes and the resultant L3 injected into dogs that were then treated with six doses of Heartgard® Plus (ivermectin + pyrantel; Merial Limited) at 30-day intervals...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143545/taz-responds-to-fluid-shear-stress-to-regulate-the-cell-cycle
#12
Hyun Jung Lee, Adesuwa Ewere, Miguel F Diaz, Pamela L Wenzel
Physical forces associated with tumor growth and drainage alter cancer cell invasiveness and metastatic potential. We previously showed that fluid frictional force, or shear stress, typical of lymphatic flow induces YAP1/TAZ activation in prostate cancer cells to promote motility dependent upon YAP1 but not TAZ. Here, we show that shear stress elevates TAZ protein levels and promotes TAZ nuclear localization. Increased TAZ activity drives increased DNA synthesis and induces AMOTL2, ANKRD1, and CTGF gene transcription independently of YAP1...
November 16, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29143242/comparing-reactive-oxygen-species-and-dna-fragmentation-in-semen-samples-of-unexplained-infertile-and-healthy-fertile-men
#13
Zahra Zandieh, Akram Vatannejad, Mahmood Doosti, Sara Zabihzadeh, Mahnaz Haddadi, Leila Bajelan, Batool Rashidi, Saeid Amanpour
BACKGROUND: Male factor infertility has increased to more than 40% during the last decade. About 30% of these couples are diagnosed with unexplained infertility. In fact, reactive oxygen species (ROS), especially superoxide anion (O2(-)·) and hydrogen peroxide (H2O2), play a crucial role in regulation of physiological and pathological processes in spermatozoa. Moreover, since the diagnosis of unexplained infertility just through semen analysis is a matter of much controversy; we aimed to evaluate the levels of ROS and sperm DNA fragmentation in the semen samples of unexplained infertile and fertile control couples...
November 15, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29143195/normal-values-of-high-resolution-manometry-in-supine-and-upright-positions-in-a-thai-population
#14
Pitichote Hiranyatheb, Suriya Chakkaphak, Supphamat Chirnaksorn, Pattaraporn Lekhaka, Kaimuk Petsrikun, Kornkanok Somboonpun
BACKGROUND: Although cut-off values used in high-resolution manometry (HRM) to diagnose esophageal motility disorders are based on representative samples of the US population and assume a supine position, differences in population and body positioning can reportedly affect results. AIMS: To establish normal HRM values for Thai people in both supine and upright positions. METHODS: Forty-one healthy subjects were recruited, each of whom underwent solid-state HRM with ten 5-mL swallows of water in both the supine and upright positions...
November 15, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29142537/pneumatic-balloon-dilatation-for-achalasia-cardia-early-late-results-a-single-center-study
#15
S Waqar H Shah, Arshad K Butt, K Malik, Altaf Alam, Anwaar A Khan
Objective: Achalasia Cardia is treated by Pneumatic balloon dilatation, Heller's Myotomy and recently, by Peroral Esophagaeal Myotomy. This study reports the efficacy of pneumatic balloon dilatation as a non-surgical motility in achieving relief of dysphagia, clinical improvement and recurrence. Long-term complications were reported. Methods: Eight hundred ninety two adult achalasia patients of both genders were treated from January 1988 till December 2011, with pneumatic balloon (Rigiflex Microvasive(®)) dilatation, under fluoroscopy Barium swallow was obtained prior to and five minutes after dilatation to evaluate for efficacy of dilatation as well as for complications...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29142433/prosopis-juliflora-pods-alkaloid-rich-fraction-in-vitro-anthelmintic-activity-on-goat-gastrointestinal-parasites-and-its-cytotoxicity-on-vero-cells
#16
Helimar Gonçalves Lima, Danilo Cavalcante Gomes, Nathália Silva Santos, Êuder Reis Dias, Mariana Borges Botura, Maria José Moreira Batatinha, Alexsandro Branco
Background: This study was designed to assess the in vitro anthelmintic activity of the fraction containing alkaloid from Prosopis juliflora pods on goat gastrointestinal nematodes using the egg hatch assay (EHA), larval migration inhibition assay (LMIA), and larval motility assay (LMA). Materials and Methods: The alkaloid-rich fraction (AF) - content juliprosopine as major alkaloid - was obtained from ethyl acetate extract after fractionation in Sephadex LH-20 chromatography column and its characterization were made by nuclear magnetic resonance analysis together with literature data comparison...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142223/active-diffusion-and-advection-in-drosophila-oocytes-result-from-the-interplay-of-actin-and-microtubules
#17
Maik Drechsler, Fabio Giavazzi, Roberto Cerbino, Isabel M Palacios
Transport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progress in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use differential dynamic microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in the Drosophila oocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows...
November 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/29142162/chloroform-extract-of-turmeric-inhibits-biofilm-formation-eps-production-and-motility-in-antibiotic-resistant-bacteria
#18
Sumreen Hayat, Anjum N Sabri, Timothy D McHugh
In the form of biofilms, bacteria exhibit more resistance to antibiotics. Biofilm formers can withstand severe conditions and the host's defense system. Therefore, it is necessary to search for effective biofilm inhibitors. In this study, we investigated the effect of a chloroform extract of turmeric on biofilm formation against antibiotic resistant bacteria. The extract exhibited its antibiofilm effect by altering adherence, motility, extracellular polymeric substance (EPS) production and cell surface hydrophobicity; important attributes of biofilm formation...
November 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29142065/maintaining-centrosomes-and-cilia
#19
REVIEW
Sascha Werner, Ana Pimenta-Marques, Mónica Bettencourt-Dias
Centrosomes and cilia are present in organisms from all branches of the eukaryotic tree of life. These structures are composed of microtubules and various other proteins, and are required for a plethora of cell processes such as structuring the cytoskeleton, sensing the environment, and motility. Deregulation of centrosome and cilium components leads to a wide range of diseases, some of which are incompatible with life. Centrosomes and cilia are thought to be very stable and can persist over long periods of time...
November 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29141997/crispr-cas9-mediated-genome-editing-reveals-the-synergistic-effects-of-%C3%AE-defensin-family-members-on-sperm-maturation-in-rat-epididymis
#20
Chaobao Zhang, Yuchuan Zhou, Shengsong Xie, Qianqian Yin, Chunhua Tang, Zimei Ni, Jian Fei, Yonglian Zhang
The epididymis is a male reproductive organ involved in posttesticular sperm maturation and storage, but the mechanism underlying sperm maturation remains unclear. β-Defensins (Defbs) belong to a family of small, cysteine-rich, cationic peptides that are antimicrobial and modulate the immune response. A large number of Defb genes are expressed abundantly in the male reproductive tract, especially in the epididymis. We and other groups have shown the involvement of several Defb genes in regulation of sperm function...
November 15, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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