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https://www.readbyqxmd.com/read/29330334/detection-of-heterozygous-mutation-in-hook-microtubule-tethering-protein-1-in-three-patients-with-decapitated-and-decaudated-spermatozoa-syndrome
#1
Huixing Chen, Yong Zhu, Zijue Zhu, Erlei Zhi, Keming Lu, Xiaobo Wang, Feng Liu, Zheng Li, Weiliang Xia
BACKGROUND: The mechanism of intramanchette transport is crucial to the transformation of sperm tail and the nuclear condensation during spermiogenesis. Although few dysfunctional proteins could result in abnormal junction between the head and tail of spermatozoon, little is known about the genetic cues in this process. OBJECTIVE: Based on patients with severe decapitated and decaudated spermatozoa (DDS) syndrome, the study aimed to validate whether new mutation exists on their Hook microtubule-tethering protein 1 (HOOK1) genes and follow their results of assisted reproduction treatment (ART)...
January 12, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29297236/cleavage-stage-embryo-micromanipulation-in-the-clinical-setting
#2
Iman Halvaei, Shahin Ghazali, Stefania A Nottola, Mohammad Ali Khalili
Embryo micromanipulation was developed after introduction of microinjection to overcome infertility. Embryo micromanipulation may be performed at any embryo stage from pronuclear to blastocyst. The technique started out as basic and turned out to be increasingly more complex. Embryo micromanipulation at the cleavage-stage includes a wide range of techniques, from opening the zona pellucida in order to improve the chance of implantation, to removing detrimental components from the embryo to enhance embryo development or blastomeres for preimplantation genetic diagnosis and embryo splitting...
January 3, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29241919/does-the-freeze-all-policy-allow-for-a-better-outcome-in-assisted-reproductive-techniques-than-the-use-of-fresh-embryo-transfers-a%C3%A2-retrospective-study-on-cumulative-live-birth-rates
#3
Jui-Chun Chang, Ming-Jer Chen, Hwa-Fen Guu, Ya-Fang Chen, Yu-Chiao Yi, Hsiao-Fan Kung, Li-Yu Chen, Min-Min Chou
OBJECTIVE: There are increasing concerns regarding the adverse effects associated with control ovarian hyperstimulation (COH) in both endometrial and uterine environments. With the "segmentation treatment policy" of assisted reproductive techniques (ART), endometrial problems may be obviated through embryo cryopreservation. However, it remains unclear if the "freeze-all policy" offers a better outcome when compared with fresh embryo transfer (ET). To clarify this, we compared the cumulative live birth rates (CLBRs) between these two patient populations...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29237055/the-epigenetic-control-of-transposable-elements-and-imprinted-genes-in-newborns-is-affected-by-the-mode-of-conception-art-versus-spontaneous-conception-without-underlying-infertility
#4
C Choux, C Binquet, V Carmignac, C Bruno, C Chapusot, J Barberet, M Lamotte, P Sagot, D Bourc'his, P Fauque
STUDY QUESTION: Do assisted reproductive technologies alter DNA methylation and/or transcription of transposable elements and imprinted genes in cord blood and placenta? SUMMARY ANSWER: After ART, DNA methylation and/or transcription changes of some transposable elements and imprinted genes were found in placenta samples while transcription modifications for some transposable elements were also discovered in cord blood. WHAT IS KNOWN ALREADY: Recent studies have confirmed the increased risk of placenta-related adverse pregnancy outcomes and the excess of imprinted disorders with abnormal methylation patterns after ART, which raises the issue of a potential ART-induced epigenetic risk...
December 11, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29229446/clinical-utility-of-decorin-in-follicular-fluid-as-a-biomarker-of-oocyte-potential
#5
Yuki Sawada, Takeshi Sato, Chieko Saito, Fumiko Ozawa, Yasuhiko Ozaki, Mayumi Sugiura-Ogasawara
This study investigated the concentration of decorin (DCN) in mature follicular fluid and the existence in the granulosa cells. It also investigated whether DCN is useful as a biomarker for outcomes of assisted reproductive technology (ART). A retrospective cohort study was performed involving 130 oocytes of 88 patients treated with ART because of unexplained infertility. The concentration of DCN in the follicular fluid (F-DCN) was 39.26ng/ml (median value); it was higher than that in serum. F-DCN of the oocytes fertilized by intracytoplasmic sperm injection (ICSI) was significantly lower than that of oocytes that were not fertilized (33...
December 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29216009/the-first-trimester-aneuploidy-biochemical-markers-in-ivf-icsi-patients-have-no-additional-benefit-compared-to-spontaneous-conceptions-in-the-prediction-of-pregnancy-complications
#6
Iwona Szymusik, Przemyslaw Kosinski, Katarzyna Kosinska-Kaczynska, Damian Warzecha, Anetta Karwacka, Bartosz Kaczynski, Miroslaw Wielgos
OBJECTIVES: The aim of this study was to determine if the levels of biochemical aneuploidy markers in in vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI) pregnancies differ from those in spontaneous pregnancies and to verify if biochemical markers could predict pregnancy outcome in IVF/ICSI gestations. METHODS: This was a prospective observational study performed in a group of 551 patients who underwent a combined first trimester prenatal screening (ultrasound scan and serum markers)...
December 7, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/29199464/sperm-rna-elements-as-markers-of-health
#7
Rayanne B Burl, Stephanie Clough, Edward Sendler, Molly Estill, Stephen A Krawetz
Idiopathic infertility, an etiology not identified as part of standard clinical assessment, represents approximately 20% of all infertility cases. Current male infertility diagnosis focuses on the concentration, motility, and morphology of spermatozoa. This is of limited value when predicting birth success and of limited utility when selecting the optimum treatment. At fertilization, spermatozoa provide their genomic contribution, as well as a set of RNAs and proteins that have distinct roles in development...
December 3, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29196493/intracytoplasmic-sperm-injection-in-domestic-and-wild-mammals
#8
Daniel Felipe Salamone, Natalia G Canel, Maria Belén Rodriguez
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical applications in the horse breeding industry. However, both ICSI blastocyst and offspring production continues to be limited for most farm and wild species. This article reviews technical differences of ICSI performance among species, possible biological and methodological reasons for the variable efficiency and potential strategies to improve the outcomes. One of the major applications of ICSI in animal production is the reproduction of high value specimens...
December 1, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29195015/does-using-testicular-sperm-retrieval-rather-than-ejaculated-spermatozoa-improve-reproductive-outcomes-in-couples-with-previous-art-failure-and-poor-ovarian-response-a-case-controlled-study
#9
A R Gilman, G Younes, S Tannus, W Y Son, P Chan, W Buckett
The objective of this study was to assess whether testicular-retrieved spermatozoa improve reproductive outcomes compared to fresh ejaculate in women with poor ovarian response and a history of previous ART failure. The study was performed as a retrospective case-control study at a university-based reproductive center in Montreal, Quebec, Canada. Eighteen poor-responder patients were matched 3 : 1 with 54 controls. Poor responders were defined as those with ≤3 oocytes retrieved at oocyte pickup. Cases were identified as poor responders, and only those with previous IVF failure(s) as an indication for testicular-retrieved spermatozoa were included...
January 2018: Andrology
https://www.readbyqxmd.com/read/29164811/congenital-heart-defects-in-ivf-icsi-pregnancy-a-systematic-review-and-meta-analysis
#10
REVIEW
Veronica Giorgione, Fabio Parazzini, Vlasta Fesslova, Sonia Cipriani, Massimo Candiani, Annalisa Inversetti, Cristina Sigismondi, Francesca Tiberio, Paolo Cavoretto
OBJECTIVE: There is no consensus in current practice guidelines whether IVF/ICSI conception represents an indication for performing a fetal echocardiogram. The aim of the study is to assess whether CHD occurs more often with the use of in-vitro-fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) techniques as compared to spontaneous pregnancies. METHODS: A systematic search for studies in PubMed/MEDLINE, Embase and Scopus from inception to September 2017 was conducted...
November 22, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29158352/intracytoplasmic-sperm-injection-state-of-the-art-in-humans
#11
REVIEW
G D Palermo, C L O'Neill, S Chow, S Cheung, A Parrella, N Pereira, Z Rosenwaks
Among infertile couples, 25% involve both male and female factors, while male factor alone accounts for another 25% due to oligo-, astheno-, teratozoospermia, a combination of the three, or even a complete absence of sperm cells in the ejaculate and can lead to a poor prognosis even with the help of assisted reproductive technology (ART). Intracytoplasmic sperm injection (ICSI) has been with us now for a quarter of a century and in spite of the controversy generated since its inception, it remains in the forefront of the techniques utilized in ART...
December 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29136648/imprinting-alterations-in-sperm-may-not-significantly-influence-art-outcomes-and-imprinting-patterns-in-the-cord-blood-of-offspring
#12
Li Tang, Zichao Liu, Ruopeng Zhang, Cunmei Su, Wenjuan Yang, Youlin Yao, Shuhua Zhao
An increase in imprinting disorders in children conceived though assisted reproductive technologies (ARTs) has been the subject of several reports. The transmission of imprinting errors from the sperm of infertile fathers is believed to be a possible reason for the increased occurrence of these disorders. However, whether the imprinting alterations in sperm affect ART outcomes and the imprinting of offspring is unclear. In the current study, we analyzed the methylation of H19, SNRPN and KCNQ1OT1 by pyrosequencing sperm samples from 97 infertile patients and 31 proven fertile males as well as cord blood samples from 13 infantswho were conceived by infertile parents through intracytoplasmic sperm injection (ICSI) and 30 healthy newborns who were conceived naturally...
2017: PloS One
https://www.readbyqxmd.com/read/29124676/cost-effectiveness-analysis-of-the-gonadotropin-treatments-hp-hmg-and-rfsh-for-assisted-reproductive-technology-in-france-a-markov-model-analysis
#13
Paul Barriere, Géraldine Porcu-Buisson, Samir Hamamah
OBJECTIVES: The objectives of this study were to assess (1) the expected cost of a live birth (LB) after in vitro fertilization with two different gonadotropin treatments [high purified human menopausal gonadotropin (HP-hMG) and recombinant follicle-stimulating hormone (rFSH)] as the single cost variable, and (2) the cost effectiveness of HP-hMG relative to rFSH in the context of the routine practice of assisted reproductive technology (ART) in France. METHODS: A Markov model was developed to simulate the therapeutic management, the in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) courses, and the effects of complications in hypothetical cohorts of 30,000 patients undergoing IVF/ICSI with fresh embryo transfer (up to four attempts) using data from the MERIT and MEGASET clinical trials or from French routine ART practice...
November 9, 2017: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/29117383/assisted-reproductive-technology-in-europe-2013-results-generated-from-european-registers-by-eshre
#14
C Calhaz-Jorge, C De Geyter, M S Kupka, J de Mouzon, K Erb, E Mocanu, T Motrenko, G Scaravelli, C Wyns, V Goossens
STUDY QUESTION: Are there any changes in the treatments involving ART and IUI initiated in Europe during 2013 compared with previous years? SUMMARY ANSWER: An increase in the overall number of ART cycles resulting from a higher number of countries reporting data was evident, the pregnancy rates (PRs) in 2013 remained stable compared with those reported in 2012, the number of transfers with multiple embryos (3+) was lower than ever before yet the multiple delivery rates (DRs) remained unchanged, and IUI activity and success rates were similar to those of last years...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29107981/prevalence-and-risk-factors-of-monochorionic-diamniotic-twinning-after-assisted-reproduction-a-six-year-experience-base-on-a-large-cohort-of-pregnancies
#15
Bing Song, Zhao-Lian Wei, Xiao-Feng Xu, Xue Wang, Xiao-Jin He, Huan Wu, Ping Zhou, Yun-Xia Cao
OBJECTIVE: To characterize the incidence and risk factors for monochorionic diamniotic(MC-DA) twinning after assisted reproductive technologies (ART). DESIGN: Retrospective population-based study. SETTING: The study was conducted in China; Department of Reproductive Medicine Center at The First Affiliated Hospital of Anhui Medical University. POPULATION: A cohort of 8860 clinical pregnancies after embryo transfer (ET) carried out in our center between 2011 and 2016 were retrospectively analyzed for the incidence of MC-DA twinning...
2017: PloS One
https://www.readbyqxmd.com/read/29073627/gene-expression-and-apoptosis-levels-in-cumulus-cells-of-patients-with-polymorphisms-of-fshr-and-lhb-undergoing-in-vitro-fertilization-program
#16
Liana Bosco, Giovanni Ruvolo, Claudio Luparello, Stefania Ferrari, Domenico Valerio, Daniele Santi, Paola Piomboni, Elena Sarcina, Monica Lispi, Maria Carmela Roccheri
BACKGROUND/AIMS: FSH receptor (FSHR) Ala307Thr and Asn680Ser and LHβ chain (LHB) Trp28Arg and Ile35Thr polymorphisms affect the response to pharmacological ovarian stimulation with r-FSH in women undergoing assisted reproductive treatment (ART). Here, we evaluated the expression level of selected genes involved in follicle maturation and the possible onset of apoptosis in cumulus cells of patients with single and double FSHR and LHB polymorphisms, as potential markers of oocyte competence...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29063502/arabian-peninsula-ethnicity-is-associated-with-lower-ovarian-reserve-and-ovarian-response-in-women-undergoing-fresh-icsi-cycles
#17
Aya M Tabbalat, Nigel Pereira, Devon Klauck, Clara Melhem, Rony T Elias, Zev Rosenwaks
PURPOSE: Recent studies have demonstrated that ethnicity can be an independent determinant of assisted reproductive technology (ART) outcomes. In this context, we investigate whether ART outcomes differ between Arabian Peninsula and Caucasian women. METHODS: This is a retrospective cohort study of women undergoing fresh intracytoplasmic sperm injection (ICSI)-embryo transfer (ET) cycles for male factor infertility. The study cohort was divided into 2 groups based on ethnicity-Arabian Peninsula or Caucasian...
October 23, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29051002/men-with-severe-oligospermia-appear-to-benefit-from-varicocele-repair-a-cost-effectiveness-analysis-of-assisted-reproductive-technology
#18
Justin M Dubin, Aubrey B Greer, Taylor P Kohn, Thomas A Masterson, Lunan Ji, Ranjith Ramasamy
OBJECTIVE: To evaluate the outcomes of men who underwent varicocelectomy for TMSC < 2 million and to perform a cost effectiveness analysis of IUI and IVF/ICSI after varicocelectomy. METHODS: We prospectively collected data of men with TMSC less than 2 million who underwent microsurgical subinguinal varicocelectomy. Men with azoospermia were excluded. Serum testosterone, luteinizing hormone, and follicle stimulating hormone were measured pre-operatively. Post-operative semen analysis was collected first at 3 months and then every 3 months thereafter...
October 16, 2017: Urology
https://www.readbyqxmd.com/read/29047006/extended-culture-of-poor-quality-supernumerary-embryos-improves-art-outcomes
#19
Amira Sallem, Pietro Santulli, Virginie Barraud-Lange, Nathalie Le Foll, Lucile Ferreux, Chloé Maignien, Mathilde Bourdon, Charles Chapron, Dominique de Ziegler, Jean-Philippe Wolf, Khaled Pocate-Cheriet
PURPOSE: The aims of this study were to investigate the possible benefits of extending the culture of poor-quality day-2 embryos (PQE) versus good-quality embryos (GQE) and to identify factors associated with pregnancy and live birth when transferring frozen-thawed blastocysts originating from GQE and PQE. METHODS: This is a retrospective cohort follow-up study performed between November 2012 and February 2015 at the IVF Laboratory Unit of Cochin University Hospital (Paris, France) including 3108 day-2 supernumerary embryos resulting from 1237 IVF/ICSI cycles...
October 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29043707/congenital-malformations-in-singleton-infants-conceived-by-assisted-reproductive-technologies-and-singleton-infants-by-natural-conception-in-tehran-iran
#20
Ramin Mozafari Kermani, Mansoureh Farhangniya, Seyed Abolhassan Shahzadeh Fazeli, Pezhman Bagheri, Mahnaz Ashrafi, Ahmad Vosough Taqi Dizaj
Background: Multiple pregnancies occur more frequently in assisted reproductive technology (ART) compared to normal conception (NC). It is known that the risk of congenital malformations in a multiple pregnancy are higher than single pregnancy. The aim of this study is to compare congenital malformations in singleton infants conceived by ART to singleton infants conceived naturally. MATERIALS AND METHODS: In this historical cohort study, we performed a historical cohort study of major congenital malformations (MCM) in 820 singleton births from January 2012 to December 2014...
January 2018: International Journal of Fertility & Sterility
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