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https://www.readbyqxmd.com/read/29778645/screening-and-selection-of-artificial-riboswitches
#1
Svetlana V Harbaugh, Jennifer Martin, Jenna Weinstein, Grant Ingram, Nancy Kelley-Loughnane
Synthetic riboswitches are engineered to regulate gene expression in response to a variety of non-endogenous small molecules, and a challenge to select this engineered response requires robust screening tools. A new synthetic riboswitch can be created by linking an in vitro-selected aptamer library with a randomized expression platform followed by in vivo selection and screening. In order to determine response to analyte, we developed a dual-color reporter comprising elements of the E. coli fimbriae phase variation system: recombinase FimE controlled by a synthetic riboswitch and an invertible DNA segment (fimS) containing a constitutively active promoter placed between two fluorescent protein genes...
May 17, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29778641/subregional-volumes-of-the-hippocampus-in-relation-to-cognitive-function-and-risk-of-dementia
#2
Tavia E Evans, Hieab H H Adams, Silvan Licher, Frank J Wolters, Aad van der Lugt, M Kamran Ikram, Michael J O'Sullivan, Meike W Vernooij, M Arfan Ikram
BACKGROUND: Total hippocampal volume has been consistently linked to cognitive function and dementia. Yet, given its complex and parcellated internal structure, the role of subregions of the hippocampus in cognition and risk of dementia remains relatively underexplored. We studied subregions of the hippocampus in a large population-based cohort to further understand their role in cognitive impairment and dementia risk. METHODS: We studied 5035 dementia- and stroke-free persons from the Rotterdam Study, aged over 45 years...
May 17, 2018: NeuroImage
https://www.readbyqxmd.com/read/29769381/tosr-mediated-regulation-of-adhesins-and-biofilm-formation-in-uropathogenic-escherichia-coli
#3
Courtney L Luterbach, Valerie S Forsyth, Michael D Engstrom, Harry L T Mobley
Uropathogenic Escherichia coli strains utilize a variety of adherence factors that assist in colonization of the host urinary tract. TosA (<u>t</u>ype <u>o</u>ne <u>s</u>ecretion <u>A</u>) is a nonfimbrial adhesin that is predominately expressed during murine urinary tract infection (UTI), binds to kidney epithelial cells, and promotes survival during invasive infections. The tosRCBDAEF operon encodes the secretory machinery necessary for TosA localization to the E...
June 27, 2018: MSphere
https://www.readbyqxmd.com/read/29767133/intestinal-challenge-with-enterotoxigenic-escherichia-coli-in-pigs-and-nutritional-intervention-to-prevent-postweaning-diarrhea
#4
REVIEW
Yawang Sun, Sung Woo Kim
Gut health of nursery pigs immediately after weaning is tightly associated with their growth performance and economic values. Postweaning diarrhea (PWD) is one of the major concerns related to gut health of nursery pigs which often is caused by infections of enterotoxigenic Escherichia coli (ETEC), mainly including F4 (K88)+ and F18+ E. coli. The main virulence factors of ETEC are adhesins (fimbriae or pili) and enterotoxins. The common types of fimbriae on ETEC from PWD pigs are F18+ and F4+ . Typically, PWD in pigs is associated with both F18+ and F4+ ETEC infections whereas pre-weaning diarrhea in pigs is associated with F4+ ETEC infection...
December 2017: Animal nutrition
https://www.readbyqxmd.com/read/29765378/epithelial-c5ar1-signaling-enhances-uropathogenic-escherichia-coli-adhesion-to-human-renal-tubular-epithelial-cells
#5
Yun Song, Kun-Yi Wu, Weiju Wu, Zhao-Yang Duan, Ya-Feng Gao, Liang-Dong Zhang, Tie Chong, Malgorzata A Garstka, Wuding Zhou, Ke Li
Recent work in a murine model of ascending urinary tract infection has suggested that C5a/C5aR1 interactions play a pathogenic role in the development of renal infection through enhancement of bacterial adhesion/colonization to renal tubular epithelial cells (RTECs). In the present study, we extended these observations to human. We show that renal tubular epithelial C5aR1 signaling is involved in promoting uropathogenic Escherichia coli (UPEC) adhesion/invasion of host cells. Stimulation of primary cultures of RTEC with C5a resulted in significant increases in UPEC adhesion/invasion of the RTEC...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29745531/-hippocampal-subfield-volume-alteration-in-post-traumatic-stress-disorder-a-magnetic-resonance-imaging-study
#6
Lu Lu, Lianqing Zhang, Xinyu Hu, Xiaoxiao Hu, Lingjiang Li, Qiyong Gong, Xiaoqi Huang
In the current study, we aim to investigate whether post-traumatic stress disorder (PTSD) is associated with structural alterations in specific subfields of hippocampus comparing with trauma-exposed control (TC) in a relatively large sample. We included 67 PTSD patients who were diagnosed under Diagnostic and Statistical Manual of Mental Disorders (4th Edition) (DSM-Ⅳ) criteria and 78 age- and sex-matched non-PTSD adult survivors who experienced similar stressors. High resolution T1 weighted images were obtained via a GE 3...
April 1, 2018: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29735381/escherichia-coli-isolates-from-patients-with-inflammatory-bowel-disease-expec-virulence-and-colicin-determinants-are-more-frequent-compared-to-healthy-controls
#7
Lenka Micenková, Lucia Frankovičová, Iva Jaborníková, Juraj Bosák, Petr Dítě, Jan Šmarda, Martin Vrba, Alena Ševčíková, Marta Kmeťová, David Šmajs
A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. The frequency of bacteriocinogenic isolates (66...
May 1, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29731507/primary-papillary-serous-carcinoma-of-the-fallopian-tube-presenting-as-a-vaginal-mass-a-case-report-and-review-of-the-literature
#8
Einav Kadour-Peero, Lena Sagi-Dain, Gil Cohen, Roman Korobochka, Abed Agbarya, Jacob Bejar, Shlomi Sagi
BACKGROUND There is now evidence to support that some cases of high-grade serous papillary carcinoma arise from the fallopian tubes rather than the ovaries. Common symptoms at presentation include abdominal pain and swelling, vomiting, altered bowel habit and urinary symptoms. To our knowledge, this is the first case of serous papillary carcinoma presenting as a vaginal mass lesion. CASE REPORT A 41-year-old woman was referred to the Bnai-Zion Medical Center with the main complaint of irregular vaginal bleeding, vaginal mucous discharge, and suspected pelvic mass...
May 7, 2018: American Journal of Case Reports
https://www.readbyqxmd.com/read/29730168/adenosine-a-2a-receptor-blockade-attenuates-spatial-memory-deficit-and-extent-of-demyelination-areas-in-lyolecithin-induced-demyelination-model
#9
Atefeh Akbari, Mohsen Khalili-Fomeshi, Manouchehr Ashrafpour, Ali Akbar Moghadamnia, Maryam Ghasemi-Kasman
In recent years, inactivation of A2A adenosine receptors has been emerged as a novel strategy for treatment of several neurodegenerative diseases. Although numerous studies have shown the beneficial effects of A2A receptors blockade on spatial memory, the impacts of selective adenosine A2A receptors on memory performance has not yet been examined in the context of demyelination. In the present study, we evaluated the effect of A2A receptor antagonist SCH58261 on spatial memory and myelination in an experimental model of focal demyelination in rat fimbria...
May 3, 2018: Life Sciences
https://www.readbyqxmd.com/read/29729028/the-sensitivity-of-a-deep-sea-fish-species-anoplopoma-fimbria-to-oil-associated-aromatic-compounds-dispersant-and-alaskan-north-slope-crude-oil
#10
Megan M McConville, John P Roberts, Myrina Boulais, Benjamin Woodall, Joshua D Butler, Aaron D Redman, Thomas F Parkerton, W Ray Arnold, Julian Guyomarch, Stéphane LeFloch, Jenny Bytingsvik, Lionel Camus, Aswani Volety, Susanne M Brander
A predominant concern following oil spills is toxicity to aquatic organisms. However, few data are available on effects in deep-sea cold water fishes. This study had three major objectives. First, investigate the relative sensitivity of the deep-sea species Anoplopoma fimbria (sablefish) to acute effects of three aromatic compounds (toluene, 2-methylnaphthalene, phenanthrene), dispersant alone and chemically enhanced water accommodated fractions (CEWAF) of Alaskan North Slope crude oil. Second, determine the critical target lipid body burden (CTLBB) for sablefish by fitting aromatic hydrocarbon toxicity data to the target lipid model (TLM), which then enabled expressing CEWAF exposures in terms of dissolved oil toxic units (TUs)...
May 5, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29712844/-escherichia-coli-cultures-maintain-stable-subpopulation-structure-during-long-term-evolution
#11
Megan G Behringer, Brian I Choi, Samuel F Miller, Thomas G Doak, Jonathan A Karty, Wanfeng Guo, Michael Lynch
How genetic variation is generated and maintained remains a central question in evolutionary biology. When presented with a complex environment, microbes can take advantage of genetic variation to exploit new niches. Here we present a massively parallel experiment where WT and repair-deficient ( ∆mutL ) Escherichia coli populations have evolved over 3 y in a spatially heterogeneous and nutritionally complex environment. Metagenomic sequencing revealed that these initially isogenic populations evolved and maintained stable subpopulation structure in just 10 mL of medium for up to 10,000 generations, consisting of up to five major haplotypes with many minor haplotypes...
April 30, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29712730/pulmonary-surfactant-promotes-virulence-gene-expression-and-biofilm-formation-in-klebsiella-pneumoniae
#12
Graham G Willsey, Sebastian Ventrone, Kristin C Schutz, Aaron M Wallace, John W Ribis, Benjamin T Suratt, Matthew J Wargo
The interactions between K lebsiella pneumoniae and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional response of K. pneumoniae MGH 78578 to purified pulmonary surfactant. This work revealed changes within the K. pneumoniae transcriptome that likely contribute to host colonization, adaptation, and virulence in vivo Notable transcripts expressed under these conditions include genes involved in capsule synthesis, LPS modification, antibiotic resistance, biofilm formation, and metabolism...
April 30, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29708991/dna-microarray-based-assessment-of-virulence-potential-of-shiga-toxin-gene-carrying-escherichia-coli-o104-h7-isolated-from-feedlot-cattle-feces
#13
Pragathi B Shridhar, Isha R Patel, Jayanthi Gangiredla, Lance W Noll, Xiaorong Shi, Jianfa Bai, Christopher A Elkins, Nancy Strockbine, T G Nagaraja
Escherichia coli O104:H4, a hybrid pathotype reported in a large 2011 foodborne outbreak in Germany, has not been detected in cattle feces. However, cattle harbor and shed in the feces other O104 serotypes, particularly O104:H7, which has been associated with sporadic cases of diarrhea in humans. The objective of our study was to assess the virulence potential of Shiga toxin-producing E. coli (STEC) O104:H7 isolated from feces of feedlot cattle using DNA microarray. Six strains of STEC O104:H7 isolated from cattle feces were analyzed using FDA-E...
2018: PloS One
https://www.readbyqxmd.com/read/29696375/genomic-characterization-of-nitrospirillum-amazonense-strain-cbamc-a-nitrogen-fixing-bacterium-isolated-from-surface-sterilized-sugarcane-stems
#14
Stefan Schwab, Leonardo Araujo Terra, José Ivo Baldani
Nitrospirillum amazonense is a nitrogen-fixing bacterium that shows potential to promote plant growth when inoculated into sugarcane and rice plants. This microorganism has been the subject of biochemical and genetic characterization to elucidate important functions related to host plant interaction and growth promotion, including the determination of draft genome sequences of two strains, Y2 and CBAmC, the second of which is the aim of the present study. CBAmC has been isolated from sugarcane (Saccharum spp...
April 25, 2018: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29692906/phenotypic-and-molecular-characterization-of-klebsiella-spp-isolates-causing-community-acquired-infections
#15
U Garza-Ramos, H Barrios-Camacho, S Moreno-Domínguez, J Toribio-Jiménez, D Jardón-Pineda, J Cuevas-Peña, A Sánchez-Pérez, J Duran-Bedolla, J Olguín-Rodriguez, A Román-Román
Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013-2014). The biochemical tests identified all as being K. pneumoniae . The molecular multiplex-PCR test identified 36 (92.4%) K. pneumoniae isolates and one (2.5%) K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1%) belonging to K. quasipneumoniae subsp. quasipneumoniae and K...
May 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29688339/4-5-years-within-patient-evolution-of-a-colistin-resistant-kpc-producing-klebsiella-pneumoniae-st258
#16
Agnès B Jousset, Rémy A Bonnin, Isabelle Rosinski-Chupin, Delphine Girlich, Gaëlle Cuzon, Nicolas Cabanel, Hélène Frech, Eric Farfour, Laurent Dortet, Philippe Glaser, Thierry Naas
Background: KPC-producing Klebsiella pneumoniae (KPC-Kp) have emerged globally over the last decade as a major nosocomial pathogen that threatens patient's care. These highly resistant bacteria are mostly associated with a single Kp clonal group (CG) CG258, but the reasons for its host and hospital adaptation remain largely unknown. Methods: We analyzed the in vivo evolution of a colistin-resistant KPC-Kp-CG258 strain that contaminated a patient following an endoscopy, and was responsible for a fatal bacteraemia 4...
April 21, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29684078/ovarian-mast-cells-migrate-toward-ovary-fimbria-connection-in-neonatal-mrl-mpj-mice
#17
Teppei Nakamura, Masataka Chihara, Osamu Ichii, Saori Otsuka-Kanazawa, Ken-Ichi Nagasaki, Yaser Hosny Ali Elewa, Osamu Tatsumi, Yasuhiro Kon
MRL/MpJ mice have abundant ovarian mast cells (MCs) as compared with other strains at postnatal day 0 (P0); however, they sharply decrease after birth. These ovarian MCs, particularly beneath the ovarian surface epithelium (SE), which express mucosal MC (MMC) marker, might participate in early follicular development. This study investigated the changes in spatiotemporal distribution of MCs in the perinatal MRL/MpJ mouse ovaries. At P0 to P7, the MCs were densely localized to the ovary, especially their caudomedial region around the ovary-fimbria connection...
2018: PloS One
https://www.readbyqxmd.com/read/29662840/activation-of-the-nlrp3-inflammasome-pathway-by-uropathogenic-escherichia-coli-is-virulence-factor-dependent-and-influences-colonization-of-bladder-epithelial-cells
#18
Isak Demirel, Alexander Persson, Annelie Brauner, Eva Särndahl, Robert Kruse, Katarina Persson
The NLRP3 inflammasome and IL-1β release have recently been suggested to be important for the progression of urinary tract infection (UTI). However, much is still unknown regarding the interaction of UPEC and the NLRP3 inflammasome. The purpose of this study was to elucidate what virulence factors uropathogenic Escherichia coli (UPEC) use to modulate NLRP3 inflammasome activation and subsequent IL-1β release and the role of NLRP3 for UPEC colonization of bladder epithelial cells. The bladder epithelial cell line 5637, CRISPR/Cas9 generated NLRP3, caspase-1 and mesotrypsin deficient cell lines and transformed primary bladder epithelial cells (HBLAK) were stimulated with UPEC isolates and the non-pathogenic MG1655 strain...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29627505/genome-analysis-of-urease-positive-serratia-marcescens-co-producing-srt-2-and-aac-6-ic-with-multidrug-efflux-pumps-for-antimicrobial-resistance
#19
Vijaya Bharathi Srinivasan, Govindan Rajamohan
In this study, we present the genome sequence of Serratia marcescens SM03, recovered from a human gut in India. The final assembly consists of 26 scaffolds (4620 coding DNA sequences, 5.08 Mb, 59.6% G + C ratio) and 79 tRNA genes. Analysis identified novel genes associated with lactose utilization, virulence, P-loop GTPases involved in urease production, CFA/I fimbriae apparatus and Yersinia - type CRISPR proteins. Antibiotic susceptibility testing indicated drug tolerant phenotype and inhibition assays demonstrated involvement of extrusion in resistance...
April 5, 2018: Genomics
https://www.readbyqxmd.com/read/29625657/multicellular-behavior-of-environmental-escherichia-coli-isolates-grown-under-nutrient-poor-and-low-temperature-conditions
#20
Laura Di Sante, Armanda Pugnaloni, Francesca Biavasco, Eleonora Giovanetti, Carla Vignaroli
The multicellular behavior designated "red dry and rough" (rdar) morphotype-characterized by production of extracellular matrix mainly comprising curli fimbriae and cellulose-is a potential survival strategy of Escherichia coli outside the host. This study documents the ability of Escherichia cryptic clades, which have recently been recognized as new lineages genetically divergent from E. coli, to grow in unfavorable conditions through expression of distinct phenotypes. Growth under low-temperature and nutrient-poor conditions induced the rdar morphotype in all cryptic clade strains tested, especially after preincubation in broth supplemented with uracil...
May 2018: Microbiological Research
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