keyword
MENU ▼
Read by QxMD icon Read
search

Canine spinal

keyword
https://www.readbyqxmd.com/read/28527111/human-placenta-derived-mesenchymal-stem-cells-loaded-on-linear-ordered-collagen-scaffold-improves-functional-recovery-after-completely-transected-spinal-cord-injury-in-canine
#1
Sufang Han, Zhifeng Xiao, Xing Li, Huan Zhao, Bin Wang, Zhixue Qiu, Zhi Li, Xin Mei, Bai Xu, Caixia Fan, Bing Chen, Jin Han, Yanzheng Gu, Huilin Yang, Qin Shi, Jianwu Dai
Traumatic spinal cord injury (SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold (LOCS) and human placenta-derived mesenchymal stem cells (hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment (control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans (CSPGs) expression...
May 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28511287/generation-and-characterization-of-highly-purified-canine-schwann-cells-from-spinal-nerve-dorsal-roots-as-potential-new-candidates-for-transplantation-strategies
#2
Nicole Steffensen, Annika Lehmbecker, Ingo Gerhauser, Yimin Wang, Regina Carlson, Andrea Tipold, Wolfgang Baumgärtner, Veronika M Stein
Schwann cells (SCs) are promising candidates for transplantation-strategies in the central nervous system by promoting axonal regeneration. The dog represents a translational model for human spinal cord injury (SCI) for studies with new repair strategies after intervertebral disk herniation (IVDH). To overcome the necessity for an additional surgical procedure, for the first time a protocol for isolation, and purification of canine SCs from spinal-nerve-biopsies during standard hemilaminectomy in IVDH affected paraplegic dogs for potential transplantation was developed...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28471303/bone-marrow-derived-mesenchymal-stem-cells-as-autologous-therapy-in-dogs-with-naturally-occurring-intervertebral-disc-disease-feasibility-safety-and-preliminary-results
#3
Frank Steffen, Lucas Smolders, Anne Roentgen, Alessandro Bertolo, Jivko Stoyanov
The intradiscal application of mesenchymal stem cells (MSC) is considered a promising strategy for intervertebral disc (IVD) regeneration. Although many studies have been published, the feasibility and regenerative effects of intradiscal MSC application has not been evaluated in an animal model suffering from naturally occurring degenerative disc disease. Six German Shepherd Dogs suffering from naturally occurring degenerative IVD disease were included. Autologous MSC were isolated from bone marrow (iliac crest) and cultured for 3 weeks...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28459053/epidural-gas-accumulation-in-connection-with-canine-degenerative-lumbosacral-disease
#4
Ditte Skytte, Hugo Schmökel
Three dogs were presented with lumbosacral hyperesthesia. Computerized tomography scans were performed in all the cases, and magnetic resonance imaging was also performed in cases 1 and 3. There was intervertebral disc (IVD) protrusion causing nerve root compression and epidural gas accumulation in all the three cases. The gas-filled cystic structures in cases 1 and 3 were within the spinal canal; in case 2, the gas was within the disc protrusion. The IVD vacuum phenomenon is relatively common in dogs, but the formation of an epidural gas accumulation in cases of a lumbar disc protrusion is rare...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28429414/evaluation-of-t2-weighted-versus-short-tau-inversion-recovery-sagittal-sequences-in-the-identification-and-localization-of-canine-intervertebral-disc-extrusion-with-low-field-magnetic-resonance-imaging
#5
Daniel Housley, Abby Caine, Giunio Cherubini, Olivier Taeymans
Sagittal T2-weighted sequences (T2-SAG) are the foundation of spinal protocols when screening for the presence of intervertebral disc extrusion. We often utilize sagittal short-tau inversion recovery sequences (STIR-SAG) as an adjunctive screening series, and experience suggests that this combined approach provides superior detection rates. We hypothesized that STIR-SAG would provide higher sensitivity than T2-SAG in the identification and localization of intervertebral disc extrusion. We further hypothesized that the parallel evaluation of paired T2-SAG and STIR-SAG series would provide a higher sensitivity than could be achieved with either independent sagittal series when viewed in isolation...
April 20, 2017: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/28401575/new-insights-into-the-neural-differentiation-potential-of-canine-adipose-tissue-derived-mesenchymal-stem-cells
#6
D Blecker, M I Elashry, M Heimann, S Wenisch, S Arnhold
Adipose tissue-derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control...
June 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/28358123/molecular-therapy-for-degenerative-disc-disease-clues-from-secretome-analysis-of-the-notochordal-cell-rich-nucleus-pulposus
#7
Ajay Matta, M Zia Karim, David E Isenman, W Mark Erwin
Degenerative disc disease (DDD) is associated with spinal pain often leading to long-term disability. However, the non-chondrodystrophic canine intervertebral disc is protected from the development of DDD, ostensibly due to its retention of notochordal cells (NC) in the nucleus pulposus (NP). In this study, we hypothesized that secretome analysis of the NC-rich NP will lead to the identification of key proteins that delay the onset of DDD. Using mass-spectrometry, we identified 303 proteins including components of TGFβ- and Wnt-signaling, anti-angiogeneic factors and proteins that inhibit axonal ingrowth in the bioactive fractions of serum free, notochordal cell derived conditioned medium (NCCM)...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28346006/magnetic-resonance-imaging-susceptibility-artifacts-in-the-cervical-vertebrae-and-spinal-cord-related-to-monocortical-screw-polymethylmethacrylate-implants-in-canine-cadavers
#8
Brian G Jones, Geoffrey T Fosgate, Eric M Green, Amy M Habing, Bianca F Hettlich
OBJECTIVE To characterize and compare MRI susceptibility artifacts related to titanium and stainless steel monocortical screws in the cervical vertebrae and spinal cord of canine cadavers. SAMPLE 12 canine cadavers. PROCEDURES Cervical vertebrae (C4 and C5) were surgically stabilized with titanium or stainless steel monocortical screws and polymethylmethacrylate. Routine T1-weighted, T2-weighted, and short tau inversion recovery sequences were performed at 3.0 T. Magnetic susceptibility artifacts in 20 regions of interest (ROIs) across 4 contiguous vertebrae (C3 through C6) were scored by use of an established scoring system...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28279204/a-study-to-compare-the-efficacy-of-polyether-ether-ketone-rod-device-with-titanium-devices-in-posterior-spinal-fusion-in-a-canine-model
#9
Nanxiang Wang, Huanxin Xie, Chunyang Xi, Han Zhang, Jinglong Yan
BACKGROUND: The benefits of posterior lumbar fusion surgery with orthotopic paraspinal muscle-pediculated bone flaps are well established. However, the problem of non-union due to mechanical support is not completely resolved. The aim of the study was to compare the efficacy of polyether ether ketone (PEEK) rod device with conventional titanium devices in the posterior lumbar fusion surgery with orthotopic paraspinal muscle-pediculated bone flaps. METHODS: This was a randomized controlled study with an experimental animal model...
March 9, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28262732/transplantation-of-huc-mscs-seeded-collagen-scaffolds-reduces-scar-formation-and-promotes-functional-recovery-in-canines-with-chronic-spinal-cord-injury
#10
Xing Li, Jun Tan, Zhifeng Xiao, Yannan Zhao, Sufang Han, Dingyang Liu, Wen Yin, Jing Li, Juan Li, Siyi Wanggou, Bing Chen, Caiping Ren, Xingjun Jiang, Jianwu Dai
Spinal cord injury (SCI) can lead to locomotor deficits, and the repair of chronic SCI is considered one of the most challenging clinical problems. Although extensive studies have evaluated treatments for acute SCI in small animals, comparatively fewer studies have been conducted on large-animal SCI in the chronic phase, which is more clinically relevant. Here, we used a collagen-based biomaterial, named the NeuroRegen scaffold, loaded with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in a canine chronic SCI model...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28253289/comparison-of-harmonic-blade-versus-traditional-approach-in-canine-patients-undergoing-spinal-decompressive-surgery-for-naturally-occurring-thoracolumbar-disk-extrusion
#11
Bianca F Hettlich, Laurie Cook, Cheryl London, Geoffrey T Fosgate
OBJECTIVES: To assess feasibility of the harmonic Osteovue blade (HOB) for use in the soft tissue approach for dogs undergoing hemilaminectomy and to compare outcomes between dogs undergoing HOB or traditional approach (TRAD). METHODS: A prospective randomized clinical trial was performed using 20 client-owned dogs with thoracolumbar intervertebral disk extrusion requiring hemilaminectomy. Dogs were randomly assigned to HOB or TRAD. Neurologic function and pain scores were assessed pre-operatively...
2017: PloS One
https://www.readbyqxmd.com/read/28240957/evaluation-of-gene-expression-and-dna-copy-number-profiles-of-adipose-tissue-derived-stromal-cells-and-consecutive-neurosphere-like-cells-generated-from-dogs-with-naturally-occurring-spinal-cord-injury
#12
Ji-Hey Lim, Sehwon Koh, Rachael Thomas, Matthew Breen, Natasha J Olby
OBJECTIVE To evaluate gene expression and DNA copy number in adipose tissue-derived stromal cells (ADSCs) and in ADSC-derived neurosphere-like cell clusters (ADSC-NSCs) generated from tissues of chronically paraplegic dogs. ANIMALS 14 client-owned paraplegic dogs. PROCEDURES Dorsal subcutaneous adipose tissue (< 1 cm(3)) was collected under general anesthesia; ADSCs were isolated and cultured. Third-passage ADSCs were cultured in neural cell induction medium to generate ADSC-NSCs. Relative gene expression of mesenchymal cell surface marker CD90 and neural progenitor marker nestin was assessed in ADSCs and ADSC-NSCs from 3 dogs by quantitative real-time PCR assay; expression of these and various neural lineage genes was evaluated for the same dogs by reverse transcription PCR assay...
March 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28230415/targeting-translational-successes-through-cansort-sci-using-pet-dogs-to-identify-effective-treatments-for-spinal-cord-injury
#13
Sarah A Moore, Nicolas Granger, Natasha J Olby, Ingo Spitzbarth, Nick D Jeffery, Andrea Tipold, Yvette S Nout-Lomas, Ronaldo C da Costa, Veronika M Stein, Linda J Noble-Haeusslein, Andrew R Blight, Robert G Grossman, D Michele Basso, Jonathan M Levine
Translation of therapeutic interventions for spinal cord injury (SCI) from laboratory to clinic has been historically challenging, highlighting the need for robust models of injury that more closely mirror the human condition. The high prevalence of acute, naturally occurring SCI in pet dogs provides a unique opportunity to evaluate expeditiously promising interventions in a population of animals that receive diagnoses and treatment clinically in a manner similar to persons with SCI, while adhering to National Institutes of Health guidelines for scientific rigor and transparent reporting...
March 22, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28192461/canine-neuroanatomy-development-of-a-3d-reconstruction-and-interactive-application-for-undergraduate-veterinary-education
#14
Hazel Raffan, Julien Guevar, Matthieu Poyade, Paul M Rea
Current methods used to communicate and present the complex arrangement of vasculature related to the brain and spinal cord is limited in undergraduate veterinary neuroanatomy training. Traditionally it is taught with 2-dimensional (2D) diagrams, photographs and medical imaging scans which show a fixed viewpoint. 2D representations of 3-dimensional (3D) objects however lead to loss of spatial information, which can present problems when translating this to the patient. Computer-assisted learning packages with interactive 3D anatomical models have become established in medical training, yet equivalent resources are scarce in veterinary education...
2017: PloS One
https://www.readbyqxmd.com/read/28190488/effect-of-a-titanium-cage-as-a-stand-alone-device-on-biomechanical-stability-in-the-lumbosacral-spine-of-canine-cadavers
#15
M Teunissen, A J van der Veen, T H Smit, M A Tryfonidou, B P Meij
Degenerative lumbosacral stenosis is a common disease in dogs characterised by intervertebral disc herniation, loss of disc height and stenosis. Decompressive dorsal laminectomy and partial discectomy can cause spinal instability and worsen foraminal stenosis. Pedicle screw and rod fixation (PSRF) with an intervertebral body cage allows for distraction and restoration of disc height and restores foraminal apertures. The aim of this study was to evaluate the ex vivo biomechanical properties of a titanium intervertebral cage alone and in combination with PSRF in the lumbosacral spine of dogs...
February 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28173745/characterization-of-chronic-axonal-degeneration-using-diffusion-tensor-imaging-in-canine-spinal-cord-injury-a-quantitative-analysis-of-dti-parameters-according-to-histopathological-differences
#16
Hakyoung Yoon, Jaehwan Kim, Won-Jin Moon, Sang-Soep Nahm, Jun Zhao, Hyoung-Moon Kim, Kidong Eom
Diffusion tensor imaging (DTI) is more sensitive than conventional magnetic resonance imaging (MRI) for the identification of axonal degeneration. However, no study to date has used DTI to evaluate the severity of axonal degeneration in canine spinal cord injury. Therefore, the aim of this study was to characterize multi-grade axonal degeneration (mild, moderate, and severe) in a canine model of spinal cord compression injury using DTI. MRI data were obtained from six normal dogs and five dogs with lumbar spinal cord injury 78 days after spinal cord injury (L1-L3) using a 3T MRI scanner...
February 7, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28132596/comparison-of-two-moldable-calcium-phosphate-based-bone-graft-materials-in-a-noninstrumented-canine-interspinous-implantation-model
#17
Davide Barbieri, Huipin Yuan, Ali Sait Ismailoğlu, Joost D de Bruijn
There is a continuing search for novel synthetic materials as an alternative to autologous bone grafting. Different technologies are explored to promote bone formation, which include the addition of BioGlass™ particles in calcium phosphate (CaP)-based materials and the use of surface modification in the form of submicron surface topographies. In this work, we aimed at comparing the bone formation in a noninstrumented canine interspinous model of moldable formulations of a submicron-surface structured tricalcium phosphate/alkylene oxide copolymer (CaP/AOC) or a tricalcium phosphate/BioGlass/collagen (CaP/BG/C) bone graft material...
February 28, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28093103/urinary-3-hydroxypropyl-mercapturic-acid-3-hpma-concentrations-in-dogs-with-acute-spinal-cord-injury-due-to-intervertebral-disc-herniation
#18
A M Sangster, L Zheng, R T Bentley, R Shi, R A Packer
The aim of this study was to investigate urinary 3-hydroxypropyl mercapturic acid (3-HPMA), a metabolite of acrolein, as a novel biomarker in acute spinal cord injury (ASCI) due to intervertebral disc herniation in dogs. Urine from 10 client-owned dogs with ASCI collected at presentation and 10 control dogs was analyzed for 3-HPMA. The median urinary 3-HPMA concentration in ASCI dogs was significantly higher than in control dogs, but was not correlated with the severity of ASCI. The median urinary 3-HPMA concentration in intact dogs was higher than in neutered dogs...
January 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28092739/an-emg-driven-biomechanical-model-of-the-canine-cervical-spine
#19
M Alizadeh, G G Knapik, J S Dufour, C Zindl, M J Allen, J Bertran, N Fitzpatrick, W S Marras
Due to the frequency of cervical spine injuries in canines, the purpose of this effort was to develop an EMG-driven dynamic model of the canine cervical spine to assess a biomechanical understanding that enables one to investigate the risk of neck disorders. A canine subject was recruited in this investigation in order to collect subject specific data. Reflective markers and a motion capture system were used for kinematic measurement; surface electrodes were used to record electromyography signals, and with the aid of force plate kinetics were recorded...
February 2017: Journal of Electromyography and Kinesiology
https://www.readbyqxmd.com/read/28074710/canine-spinal-cord-glioma
#20
Daniel R Rissi, Renee Barber, Annabelle Burnum, Andrew D Miller
Spinal cord glioma is uncommonly reported in dogs. We describe the clinicopathologic and diagnostic features of 7 cases of canine spinal cord glioma and briefly review the veterinary literature on this topic. The median age at presentation was 7.2 y. Six females and 1 male were affected and 4 dogs were brachycephalic. The clinical course lasted from 3 d to 12 wk, and clinical signs were progressive and associated with multiple suspected neuroanatomic locations in the spinal cord. Magnetic resonance imaging of 6 cases revealed T2-weighted hyperintense lesions with variable contrast enhancement in the spinal cord...
January 2017: Journal of Veterinary Diagnostic Investigation
keyword
keyword
105928
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"