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https://www.readbyqxmd.com/read/28324838/influence-of-multi-walled-carbon-nanotubes-on-the-effects-of-roxithromycin-in-crucian-carp-carassius-auratus-in-the-presence-of-natural-organic-matter
#1
Zhenhua Yan, Guanghua Lu, Hongwei Sun, Binni Ma
Carbon nanotubes are increasingly entering the aquatic environment and may interact with other co-existing contaminants, such as antibiotics. However, whether these interactions may affect their bioavailability in aquatic organisms is the subject of considerable debate. The primary objective of this study was to assess the risks arising from the coexistence of roxithromycin (ROX) and multi-walled carbon nanotubes (MWCNTs) in waters containing natural organic matter (NOM), focusing on the distribution and bioaccumulation of ROX in crucian carp (Carassius auratus), and the related biochemical status...
March 11, 2017: Chemosphere
https://www.readbyqxmd.com/read/28288446/hybrid-detection-of-target-sequence-dna-based-on-phosphorescence-resonance-energy-transfer
#2
Yanming Miao, Jinzhi Lv, Guiqin Yan
The severe background fluorescence and scattering light of real biological samples or environmental samples largely reduce the sensitivity and accuracy of fluorescence resonance energy transfer sensors based on fluorescent quantum dots (QDs). To solve this problem, we designed a novel target sequence DNA biosensor based on phosphorescent resonance energy transfer (PRET). This sensor relied on Mn-doped ZnS (Mn-ZnS) room-temperature phosphorescence (RTP) QDs/poly-(diallyldimethylammonium chloride) (PDADMAC) nanocomposite (QDs(+)) as the energy donor and the single-strand DNA-ROX as the energy receptor...
March 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28252677/increasing-clinical-faculty-engagement-in-problem-driven-research-the-magic-wand-initiative-at-massachusetts-general-hospital
#3
Lilit Garibyan, R Rox Anderson
No abstract text is available yet for this article.
March 2, 2017: JAMA Dermatology
https://www.readbyqxmd.com/read/28250665/erratum-bioactivity-of-diterpens-from-the-ethyl-acetate-extract-of-kingiodendron-pinnatum-rox-hams
#4
(no author information available yet)
[This corrects the article on p. 287 in vol. 8, PMID: 27695270.].
January 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28244201/overexpression-of-oserf48-causes-regulation-of-oscml16-a-calmodulin-like-protein-gene-that-enhances-root-growth-and-drought-tolerance
#5
Harin Jung, Pil Joong Chung, Su-Hyun Park, Mark Christian Felipe Reveche Redillas, Youn Shic Kim, Joo-Won Suh, Ju-Kon Kim
The AP2/ERF family is a plant-specific transcription factor (TF) family whose members have been associated with various developmental processes and stress tolerance. Here, we functionally characterized the drought-inducible OsERF48, a group Ib member of the rice ERF family with four conserved motifs, CMI-1, -2, -3 and -4. A transactivation assay in yeast revealed that the C-terminal CMI-1 motif was essential for OsERF48 transcriptional activity. When OsERF48 was overexpressed in an either a root-specific (ROX(OsERF48) ) or whole-body (OX(OsERF48) ) manner, transgenic plants showed a longer and denser root phenotype compared to the nontransgenic (NT) controls...
February 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28225598/novel-aptasensor-platform-based-on-ratiometric-surface-enhanced-raman-spectroscopy
#6
Yan Wu, Fubing Xiao, Zhaoyang Wu, Ruqin Yu
A novel aptasensor platform has been developed for quantitative detection of adenosine triphosphate (ATP) based on a ratiometric surface-enhanced Raman scattering (SERS) strategy. The thiolated 3'-Rox-labeled complementary DNA (cDNA) is first immobilized on the gold nanoparticle (AuNP) surface and then hybridizes with the 3'-Cy5-labeled ATP-binding aptamer probe (Cy5-aptamer) to form a rigid double-stranded DNA (dsDNA), in which the Cy5 and Rox Raman labels are used to produce the ratiometric Raman signals...
February 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28219238/public-health-risk-of-arsenic-species-in-chicken-tissues-from-live-poultry-markets-of-guangdong-province-china
#7
Yuanan Hu, Wenfeng Zhang, Hefa Cheng, Shu Tao
Arsenic-based feed additives, such as roxarsone (ROX), are still legally and widely used in food animal production in many countries. This study was conducted to systematically characterize the content and speciation of arsenic in chicken tissues from live poultry markets and in commercial chicken feeds in Guangdong, a major poultry production and consumption province in China, and to assess the corresponding public health risk. The total arsenic contents in the commercial feeds could be modeled as a mixture of two log-normal distributions (geometric means: 0...
March 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28117942/the-effect-of-simulated-inflammatory-conditions-and-fenton-chemistry-on-the-electrochemistry-of-cocrmo-alloy
#8
Yangping Liu, Jeremy L Gilbert
Inflamed conditions may develop in total joint replacement applications and may impact on the corrosion of metallic biomaterials like CoCrMo alloy. The influence of simulated inflammatory (SI) conditions on the corrosion behavior of CoCrMo alloy was investigated. A range of SI solutions, based on phosphate buffered saline with H2 O2 , HCl, and Fe(3+) additions, were investigated. Open circuit potential (OCP), corrosion currents and impedance of the oxide film surface were all significantly (p < 0.05) affected by increases in H2 O2 concentration and decrease of pH...
January 24, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28089757/disorazoles-block-group-a-streptococcal-invasion-into-epithelial-cells-via-interference-with-the-host-factor-ezrin
#9
Katharina Rox, Manfred Rohde, Gursharan Singh Chhatwal, Rolf Müller
Bacterial pathogens use invasion into human cells as a strategy to escape not only the host's immune response, but also anti-bacterial treatment. This often leads to persistence and enables reinitiation of the infection process at a later time point. Here, we show that a family of myxobacterial metabolites, disorazoles, block invasion of group A Streptococcus (GAS) into human epithelial cells. Mechanistically, disorazoles target ezrin, a host protein involved in linking microfilaments to the membrane, and affect invasion most likely by interfering with dynamic phosphorylation of ezrin...
January 5, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28089399/a-mass-balance-model-to-estimate-the-rate-of-composting-methane-oxidation-and-anaerobic-digestion-in-soil-covers-and-shallow-waste-layers
#10
Reza Rafiee, Lizanne Obersky, Sihuang Xie, William P Clarke
Although CH4 oxidation in landfill soil covers is widely studied, the extent of composting and CH4 oxidation in underlying waste layers has been speculated but not measured. The objective of this study was to develop and validate a mass balance model to estimate the simultaneous rates of anaerobic digestion (rAD), CH4 oxidation (rOX) and composting (rCOM) in environments where O2 penetration is variable and zones of aerobic and anaerobic activity are intermingled. The modelled domain could include, as an example, a soil cover and the underlying shallow waste to a nominated depth...
January 12, 2017: Waste Management
https://www.readbyqxmd.com/read/28035495/molecular-cloning-phylogenetic-analysis-and-expression-patterns-of-lateral-suppressor-like-and-regulator-of-axillary-meristem-formation-like-genes-in-sunflower-helianthus-annuus-l
#11
Marco Fambrini, Mariangela Salvini, Claudio Pugliesi
The wild sunflower (Helianthus annuus) plants develop a highly branched form with numerous small flowering heads. The origin of a no branched sunflower, producing a single large head, has been a key event in the domestication process of this species. The interaction between hormonal factors and several genes organizes the initiation and outgrowth of axillary meristems (AMs). From sunflower, we have isolated two genes putatively involved in this process, LATERAL SUPPRESSOR (LS)-LIKE (Ha-LSL) and REGULATOR OF AXILLARY MERISTEM FORMATION (ROX)-LIKE (Ha-ROXL), encoding for a GRAS and a bHLH transcription factor (TF), respectively...
December 29, 2016: Development Genes and Evolution
https://www.readbyqxmd.com/read/28007166/comparative-cytotoxicity-of-fourteen-trivalent-and-pentavalent-arsenic-species-determined-using-real-time-cell-sensing
#12
Birget Moe, Hanyong Peng, Xiufen Lu, Baowei Chen, Lydia W L Chen, Stephan Gabos, Xing-Fang Li, X Chris Le
The occurrence of a large number of diverse arsenic species in the environment and in biological systems makes it important to compare their relative toxicity. The toxicity of arsenic species has been examined in various cell lines using different assays, making comparison difficult. We report real-time cell sensing of two human cell lines to examine the cytotoxicity of fourteen arsenic species: arsenite (As(III)), monomethylarsonous acid (MMA(III)) originating from the oxide and iodide forms, dimethylarsinous acid (DMA(III)), dimethylarsinic glutathione (DMAG(III)), phenylarsine oxide (PAO(III)), arsenate (As(V)), monomethylarsonic acid (MMA(V)), dimethylarsinic acid (DMA(V)), monomethyltrithioarsonate (MMTTA(V)), dimethylmonothioarsinate (DMMTA(V)), dimethyldithioarsinate (DMDTA(V)), 3-nitro-4-hydroxyphenylarsonic acid (Roxarsone, Rox), and 4-aminobenzenearsenic acid (p-arsanilic acid, p-ASA)...
November 2016: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28006942/propofol-induced-mitochondrial-and-contractile-dysfunction-of-the-rat-ventricular-myocardium
#13
M Grundmanová, D Jarkovská, A Süß, Z Tůma, M Marková, Z Grundman, A El-Kadi, M Čedíková, M Štengl, J Kuncová
Propofol is a short-acting hypnotic agent used in human medicine for sedation and general anesthesia. Its administration can be associated with serious cardiovascular side-effects that include decrease in arterial blood pressure and cardiac output. The aim of the present study was to evaluate propofol effects on mitochondrial respiration, myocardial contractility and electrophysiology in the same samples isolated from the heart ventricles of adult rats. Mitochondrial oxygen consumption was measured in permeabilized samples dissected from free walls of both ventricles using high-resolution respirometry...
December 22, 2016: Physiological Research
https://www.readbyqxmd.com/read/28003251/effect-of-arteriovenous-anastomosis-on-blood-pressure-reduction-in-patients-with-isolated-systolic-hypertension-compared-with-combined-hypertension
#14
Christian Ott, Melvin D Lobo, Paul A Sobotka, Felix Mahfoud, Alice Stanton, John Cockcroft, Neil Sulke, Eamon Dolan, Markus van der Giet, Joachim Hoyer, Stephen S Furniss, John P Foran, Adam Witkowski, Andrzej Januszewicz, Danny Schoors, Konstantinos Tsioufis, Benno J Rensing, Manish Saxena, Benjamin Scott, G André Ng, Stephan Achenbach, Roland E Schmieder
BACKGROUND: Options for interventional therapy to lower blood pressure (BP) in patients with treatment-resistant hypertension include renal denervation and the creation of an arteriovenous anastomosis using the ROX coupler. It has been shown that BP response after renal denervation is greater in patients with combined hypertension (CH) than in patients with isolated systolic hypertension (ISH). We analyzed the effect of ROX coupler implantation in patients with CH as compared with ISH...
December 21, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27997018/biomedical-optics-centers-forty-years-of-multidisciplinary-clinical-translation-for-improving-human-health
#15
Bruce J Tromberg, R Rox Anderson, Reginald Birngruber, Ralf Brinkmann, Michael W Berns, John A Parrish, Gabriela Apiou-Sbirlea
No abstract text is available yet for this article.
December 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27995349/the-essential-drosophila-clamp-protein-differentially-regulates-non-coding-rox-rnas-in-male-and-females
#16
Jennifer A Urban, Caroline A Doherty, William T Jordan, Jacob E Bliss, Jessica Feng, Marcela M Soruco, Leila E Rieder, Maria A Tsiarli, Erica N Larschan
Heterogametic species require chromosome-wide gene regulation to compensate for differences in sex chromosome gene dosage. In Drosophila melanogaster, transcriptional output from the single male X-chromosome is equalized to that of XX females by recruitment of the male-specific lethal (MSL) complex, which increases transcript levels of active genes 2-fold. The MSL complex contains several protein components and two non-coding RNA on the X ( roX) RNAs that are transcriptionally activated by the MSL complex. We previously discovered that targeting of the MSL complex to the X-chromosome is dependent on the chromatin-linked adapter for MSL proteins (CLAMP) zinc finger protein...
December 19, 2016: Chromosome Research
https://www.readbyqxmd.com/read/27916881/optimization-of-cyanine-dye-stability-and-analysis-of-fret-interaction-on-dna-microarrays
#17
Marcel von der Haar, Christopher Heuer, Martin Pähler, Kathrin von der Haar, Patrick Lindner, Thomas Scheper, Frank Stahl
The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores' susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS)...
November 30, 2016: Biology
https://www.readbyqxmd.com/read/27861257/pulmonary-phototherapy-to-treat-carbon-monoxide-poisoning-in-rats
#18
Luca Zazzeron, Chen Liu, Walfre Franco, Akito Nakagawa, William A Farinelli, Donald B Bloch, R Rox Anderson, Warren M Zapol
BACKGROUND: Carbon monoxide (CO) poisoning is a common cause of poison-related mortality. CO binds to hemoglobin in the blood to form carboxyhemoglobin (COHb), impairing oxygen delivery to peripheral tissues. Current treatment of CO poisoned patients involves oxygen administration to rapidly remove CO and restore oxygen delivery. Light dissociates CO from COHb with high efficiency. Exposure of murine lungs to visible laser-generated light improved the CO elimination rate in vivo. The aims of this study were to apply pulmonary phototherapy to a larger animal model of CO poisoning, to test novel approaches to light delivery, and to examine the effect of chemiluminescence-generated light on the CO elimination rate...
November 16, 2016: Shock
https://www.readbyqxmd.com/read/27835937/fractional-laser-assisted-topical-delivery-leads-to-enhanced-accelerated-and-deeper-cutaneous-5-fluorouracil-uptake
#19
Emily Wenande, Uffe H Olesen, Mette M B Nielsen, Christian Janfelt, Steen Honoré Hansen, R Rox Anderson, Merete Haedersdal
BACKGROUND: Topical 5-Fluorouracil (5-FU) exhibits suboptimal efficacy for non-melanoma skin cancer, attributed to insufficient intracutaneous penetration. This study investigates the impact of ablative fractional laser (AFXL) at different laser-channel depths on cutaneous 5-FU pharmacokinetics and biodistribution. METHODS: In vitro porcine skin underwent AFXL-exposure using a fractional 10,600 nm CO2-laser, generating microscopic ablation zones (MAZ) reaching the dermoepidermal junction (MAZ-ED), superficial-(MAZ-DS), or mid-dermis(MAZ-DM)...
November 28, 2016: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/27814537/phytotoxicity-and-uptake-of-roxarsone-by-wheat-triticum-aestivum-l-seedlings
#20
Qing-Long Fu, Lee Blaney, Dong-Mei Zhou
Roxarsone (ROX), the primary aromatic arsenical additive (AAA) used in animal feeding operations, is of increasing concern to environmental and human health due to land application of ROX-laden animal manure. Few studies have investigated the phytotoxicity, uptake mechanisms, and speciation of AAA in crop plants. In this study, wheat seedlings were employed to address these issues under hydroponic conditions. Compared to inorganic arsenic, ROX was less toxic to wheat root elongation. Wheat roots were more sensitive to ROX stress than shoots...
December 2016: Environmental Pollution
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