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https://www.readbyqxmd.com/read/29131159/enhancing-the-precision-of-genetic-lineage-tracing-using-dual-recombinases
#1
Lingjuan He, Yan Li, Yi Li, Wenjuan Pu, Xiuzhen Huang, Xueying Tian, Yue Wang, Hui Zhang, Qiaozhen Liu, Libo Zhang, Huan Zhao, Juan Tang, Hongbin Ji, Dongqing Cai, Zhibo Han, Zhongchao Han, Yu Nie, Shengshou Hu, Qing-Dong Wang, Ruilin Sun, Jian Fei, Fengchao Wang, Ting Chen, Yan Yan, Hefeng Huang, William T Pu, Bin Zhou
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing...
November 13, 2017: Nature Medicine
https://www.readbyqxmd.com/read/29107578/facile-detection-of-microrna-based-on-phosphorescence-resonance-energy-transfer-and-duplex-specific-nuclease-assisted-signal-amplification
#2
Jia-Jia Yang, Zhi-Feng Zhang, Gui-Qin Yan
MicroRNAs (miRNAs) play an important role in many biological processes, and its level in plasma and other biological fluids is closely related to many diseases. In this work, a selective room-temperature phosphorescence (RTP) detection method for miRNA was developed based on a duplex-specific nuclease (DSN) -assisted signal amplification strategy and phosphorescence resonance energy transfer (PRET) between poly-diallyldimethylammonium chloride-modified quantum dots (QDs@PDDA) and 6-carboxy-X-rhodamine-modified miRNA sequences complementary oligonucleotide (ROX-ssDNA)...
October 28, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29084002/central-arteriovenous-anastomosis-to-treat-resistant-hypertension
#3
Vikas Kapil, Paul A Sobotka, Mel D Lobo, Roland E Schmieder
PURPOSE OF REVIEW: Novel, interventional treatments have emerged in the last decade for the treatment of resistant hypertension. This review focuses on a unique device that creates a fixed calibre, central iliac arteriovenous anastomosis that is significantly different in haemodynamic and safety profile from traditional haemodialysis fistulae. The background, physiology, and clinical data to date will be presented. RECENT FINDINGS: The single, randomized, controlled clinical trial using the ROX coupler in patients with resistant hypertension demonstrated substantial reductions in both office and ambulatory blood pressure to 12-month postimplantation...
October 27, 2017: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/29081897/mitochondrial-respiration-in-intact-peripheral-blood-mononuclear-cells-and-sirtuin-3-activity-in-patients-with-movement-disorders
#4
Slawomir Michalak, Jolanta Florczak-Wyspiańska, Joanna Rybacka-Mossakowska, Wojciech Ambrosius, Krystyna Osztynowicz, Aleksandra Baszczuk, Wojciech Kozubski, Ewa Wysocka
OBJECTIVE: Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders. Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties. The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the SIRT3 activity in patients with movement disorders. METHODS: Mitochondrial respiration was analyzed in intact PBMCs using the ROUTINE, LEAK, electron transfer system (ETS), and residual oxygen consumption (ROX) protocol by means of high-resolution respirometry...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29074235/transformation-of-roxarsone-in-the-anoxic-oxic-process-when-treating-the-livestock-wastewater
#5
Yue Yin, Junfeng Wan, Shaozhen Li, Hongli Li, Christophe Dagot, Yan Wang
In order to evaluate the influence of roxarsone (ROX) on the livestock wastewater treatment, a lab-scale pilot employing an anoxic-oxic (A-O) process was investigated by adding different concentrations of ROX at different periods. The mass balance of arsenic (As) in the A-O system was established through the analysis of As speciation and As migration in the gas, liquid and solid phases. The results showed that around 80% of total ROX (initial concentration was 50mgROXL(-1)) was eliminated in the anoxic reactor (R1) in which at least about 11% of total ROX was transformed to inorganic As(v) (iAs(v)) due to the direct breaking of the C-As bond of ROX...
October 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29066499/a-mutually-exclusive-stem-loop-arrangement-in-rox2-rna-is-essential-for-x-chromosome-regulation-in-drosophila
#6
Ibrahim Avsar Ilik, Daniel Maticzka, Plamen Georgiev, Noel Marie Gutierrez, Rolf Backofen, Asifa Akhtar
The X chromosome provides an ideal model system to study the contribution of RNA-protein interactions in epigenetic regulation. In male flies, roX long noncoding RNAs (lncRNAs) harbor several redundant domains to interact with the ubiquitin ligase male-specific lethal 2 (MSL2) and the RNA helicase Maleless (MLE) for X-chromosomal regulation. However, how these interactions provide the mechanics of spreading remains unknown. By using the uvCLAP (UV cross-linking and affinity purification) methodology, which provides unprecedented information about RNA secondary structures in vivo, we identified the minimal functional unit of roX2 RNA...
October 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/29061728/central-iliac-arteriovenous-anastomosis-for-uncontrolled-hypertension-one-year-results-from-the-rox-control-htn-trial
#7
Melvin D Lobo, Christian Ott, Paul A Sobotka, Manish Saxena, Alice Stanton, John R Cockcroft, Neil Sulke, Eamon Dolan, Markus van der Giet, Joachim Hoyer, Stephen S Furniss, John P Foran, Adam Witkowski, Andrzej Januszewicz, Danny Schoors, Konstantinos Tsioufis, Benno J Rensing, Benjamin Scott, G André Ng, Roland E Schmieder
Creation of a central iliac arteriovenous anastomosis using a novel nitinol coupler device results in an immediate, significant reduction of blood pressure (BP). We present efficacy and safety findings at 12 months post-coupler insertion. This open-label, multicenter, prospective, randomized trial enrolled patients with a baseline office systolic BP ≥140 mm Hg and average daytime ambulatory BP ≥135/85 mm Hg. Subjects were randomly allocated to coupler implantation and continuing previous pharmacotherapy or to maintain previous treatment alone...
October 23, 2017: Hypertension
https://www.readbyqxmd.com/read/29061721/hypertension-on-the-rox-durable-blood-pressure-lowering-with-central-iliac-arteriovenous-anastomosis
#8
EDITORIAL
Markus P Schlaich, Omar Azzam, Yusuke Sata
No abstract text is available yet for this article.
October 23, 2017: Hypertension
https://www.readbyqxmd.com/read/29058788/significant-skin-tightening-by-closure-of-fractional-ablative-laser-holes
#9
Elisabeth Russe, Martin Purschke, Wikunda Limpiangkanan, William A Farinelli, Ying Wang, Apostolos G Doukas, Fernanda H Sakamoto, Gottfried Wechselberger, Richard Rox Anderson
BACKGROUND AND OBJECTIVE: Ablative fractional laser treatment uses thousands of very small laser beam wounds to damage a fraction of the skin, which stimulates tissue remodeling. Each open micro-wound heals without scarring, but the amount of skin tightening achieved is limited. This animal study was performed to test the hypothesis that immediate temporary closure of fractional laser wounds could increase skin tightening after fractional ablative laser treatment. MATERIALS AND METHODS: Four adult swine were used for the study; 98 square test sites (3 × 3 cm) were tattooed on the abdomen and flanks of each pig...
October 23, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29045384/disorder-in-convergent-floral-nanostructures-enhances-signalling-to-bees
#10
Edwige Moyroud, Tobias Wenzel, Rox Middleton, Paula J Rudall, Hannah Banks, Alison Reed, Greg Mellers, Patrick Killoran, M Murphy Westwood, Ullrich Steiner, Silvia Vignolini, Beverley J Glover
Diverse forms of nanoscale architecture generate structural colour and perform signalling functions within and between species. Structural colour is the result of the interference of light from approximately regular periodic structures; some structural disorder is, however, inevitable in biological organisms. Is this disorder functional and subject to evolutionary selection, or is it simply an unavoidable outcome of biological developmental processes? Here we show that disordered nanostructures enable flowers to produce visual signals that are salient to bees...
October 26, 2017: Nature
https://www.readbyqxmd.com/read/29019392/one-step-synthesis-of-rox-dna-functionalized-cdzntes-quantum-dots-for-the-visual-detection-of-hydrogen-peroxide-and-blood-glucose
#11
Guobin Mao, Qin Cai, Fubing Wang, Changliang Luo, Xinghu Ji, Zhike He
As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of H2O2 and glucose...
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28952731/elucidating-the-stimulatory-and-inhibitory-effects-of-dissolved-organic-matter-from-poultry-litter-on-photodegradation-of-antibiotics
#12
Kiranmayi P Mangalgiri, Lee Blaney
This study examined the photolytic fate of the chlortetracycline (CTC), ciprofloxacin (CIP), roxarsone (ROX), and sulfamethoxazole (SMX) antibiotics in agriculturally relevant matrices. The observed photodegradation kinetics for antibiotics in solutions containing dissolved organic matter (DOM) from three poultry litter extracts was modeled to identify contributions from direct and indirect photolysis. Suwannee River natural organic matter (SRN) was used as a surrogate DOM standard. Poultry litter-derived DOM generated lower concentrations of reactive species compared to SRN...
November 7, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28951240/skin-surface-temperatures-measured-by-thermal-imaging-aid-in-the-diagnosis-of-cellulitis
#13
Lauren N Ko, Adam B Raff, Anna C Garza-Mayers, Allison S Dobry, Antonio Ortega-Martinez, R Rox Anderson, Daniela Kroshinsky
Warmth is a characteristic but non-diagnostic feature of cellulitis. We assessed the diagnostic utility of skin surface temperature in differentiating cellulitis from pseudocellulitis. Adult patients presenting to the emergency department of a large urban hospital with presumed cellulitis were enrolled. Patients were randomized to dermatology consultation(n=40) versus standard of care(n=32). Thermal images of affected and unaffected skin were obtained for each patient. Analysis was performed on dermatology consultation patients to establish a predictive model for cellulitis, which was then validated in the other cohort...
September 23, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28936873/biochemical-characterization-of-arsi-a-novel-c-as-lyase-for-degradation-of-environmental-organoarsenicals
#14
Shashank S Pawitwar, Venkadesh S Nadar, Ashoka Kandegedara, Timothy L Stemmler, Barry P Rosen, Masafumi Yoshinaga
Organoarsenicals such as the methylarsenical methylarsenate (MAs(V)) and aromatic arsenicals including roxarsone (4-hydroxy-3-nitrobenzenearsenate or Rox(V)) have been extensively used as an herbicide and growth enhancers in animal husbandry, respectively. They undergo environmental degradation to more toxic inorganic arsenite (As(III)) that contaminates crops and drinking water. We previously identified a bacterial gene (arsI) responsible for aerobic demethylation of methylarsenite (MAs(III)). The gene product, ArsI, is an Fe(II)-dependent extradiol dioxygenase that cleaves the carbon-arsenic (C-As) bond in MAs(III) and in trivalent aromatic arsenicals...
September 22, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28924140/linoleic-and-palmitoleic-acid-block-streptokinase-mediated-plasminogen-activation-and-reduce-severity-of-invasive-group-a-streptococcal-infection
#15
Katharina Rox, Rolf Jansen, Torsten G Loof, Christine M Gillen, Steffen Bernecker, Mark J Walker, Gursharan Singh Chhatwal, Rolf Müller
In contrast to mild infections of Group A Streptococcus (GAS) invasive infections of GAS still pose a serious health hazard: GAS disseminates from sterile sites into the blood stream or deep tissues and causes sepsis or necrotizing fasciitis. In this case antibiotics do not provide an effective cure as the bacteria are capable to hide from them very quickly. Therefore, new remedies are urgently needed. Starting from a myxobacterial natural products screening campaign, we identified two fatty acids isolated from myxobacteria, linoleic and palmitoleic acid, specifically blocking streptokinase-mediated activation of plasminogen and thereby preventing streptococci from hijacking the host's plasminogen/plasmin system...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28892855/oxidizing-capacity-of-the-rural-atmosphere-in-hong-kong-southern-china
#16
Zeyuan Li, Likun Xue, Xue Yang, Qiaozhi Zha, Yee Jun Tham, Chao Yan, Peter K K Louie, Connie W Y Luk, Tao Wang, Wenxing Wang
Atmospheric oxidizing capacity (AOC), dominated by the hydroxyl radical (OH), is an important index of the self-cleaning capacity of atmosphere and plays a vital role in the tropospheric chemistry. To better understand the key processes governing the chemistry of rural atmosphere of southern China, we analyzed the oxidation capacity and radical chemistry at a regional background site in Hong Kong from 23 August to 22 December 2012, which covered the summer, autumn and winter seasons. A chemical box model built on the latest Master Chemical Mechanism (v3...
January 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28882037/reusable-silicon-based-surface-enhanced-raman-scattering-ratiometric-aptasensor-with-high-sensitivity-specificity-and-reproducibility
#17
Huayi Shi, Na Chen, Yuanyuan Su, Houyu Wang, Yao He
Rapid, sensitive, and accurate detection of adenosine triphosphate (ATP), the primary energy molecule, is critical for the elucidation of its unique roles in cell signaling and many cellular reactions. Up to date, a major challenge is still remaining for fabricating surface-enhanced Raman scattering (SERS) aptamer sensors (aptasensors) suitable for accurate and reliable quantification of ATP. Herein, we develop the ratiometric silicon SERS aptasensor for ATP detection, which is made of uniform silver nanoparticles (Ag NPs)-modified silicon wafer (Ag NPs@Si), followed by the functionalization with double-stranded DNA (dsDNA I)...
September 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28851915/poisson-plus-quantification-for-digital-pcr-systems
#18
Nivedita Majumdar, Swapnonil Banerjee, Michael Pallas, Thomas Wessel, Patricia Hegerich
Digital PCR, a state-of-the-art nucleic acid quantification technique, works by spreading the target material across a large number of partitions. The average number of molecules per partition is estimated using Poisson statistics, and then converted into concentration by dividing by partition volume. In this standard approach, identical partition sizing is assumed. Violations of this assumption result in underestimation of target quantity, when using Poisson modeling, especially at higher concentrations. The Poisson-Plus Model accommodates for this underestimation, if statistics of the volume variation are well characterized...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28843197/novel-ratiometric-surface-enhanced-raman-spectroscopy-aptasensor-for-sensitive-and-reproducible-sensing-of-hg-2
#19
Yan Wu, Tingting Jiang, Zhaoyang Wu, Ruqin Yu
It is important to precisely monitor mercury (II) ions (Hg(2+)) for environment protection and human health monitoring. Although many strategies have been developed in the past decades, there still remains a challenge for developing an ultrasensitive, simple and reliable approach to detect Hg(2+). Herein, we report a ratiometric surface-enhanced Raman scattering (SERS) aptasensor by employing aptamer-modified Au@Ag core-shell nanoparticles (Au@Ag NPs) as highly functional sensing probes, allowing for ultrasensitive detection of Hg(2+)...
August 19, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28831103/a-novel-fluorescent-imaging-technique-for-assessment-of-cerebral-vasospasm-after-experimental-subarachnoid-hemorrhage
#20
Diane J Aum, Ananth K Vellimana, Itender Singh, Eric Milner, James W Nelson, Byung Hee Han, Gregory J Zipfel
Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye...
August 22, 2017: Scientific Reports
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