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https://www.readbyqxmd.com/read/28469250/development-of-a-novel-zebrafish-model-for-type-2-diabetes-mellitus
#1
Liqing Zang, Yasuhito Shimada, Norihiro Nishimura
Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using an automated feeding system. We monitored the fasting blood glucose levels in the normal-fed group (one feed/day) and overfed group (six feeds/day) over an 8-week period. The fasting blood glucose level was significantly increased in DIO zebrafish compared with that of normal-fed zebrafish...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28457443/dextran-production-by-lactobacillus-sakei-mn1-coincides-with-reduced-autoagglutination-biofilm-formation-and-epithelial-cell-adhesion
#2
Montserrat Nácher-Vázquez, Iñaki Iturria, Kenza Zarour, Maria Luz Mohedano, Rosa Aznar, Miguel Ángel Pardo, Paloma López
In this work we have investigated two dextran-producing lactic acid bacteria, Lactobacillus sakei MN1 and Leuconostoc mesenteroides RTF10, isolated from fermented meat products. These bacteria synthesise dextran when sucrose, but not glucose, is present in the growth medium. The influence of dextran on bacterial aggregation, adhesion and biofilm formation was investigated in cultures challenged with sucrose or glucose. For Lb. sakei MN1, the synthesis of the dextran drastically impaired the three processes; in contrast it had no effect on Lc...
July 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28443032/fatty-acid-binding-protein-11a-is-required-for-brain-vessel-integrity-in-zebrafish
#3
Jie Zhang, Jialing Qi, Shuilong Wu, Lijiao Peng, Yunwei Shi, Jinxian Yang, Zhenhua Yin, Yu Gao, Chengniu Wang, Jie Gong, Haijun Zhang, Jingjing Zhang, Dong Liu
The monolayer of endothelial cells (ECs) lining the intima of all blood vessel wall forms a semipermeable barrier that regulates tissue-fluid homeostasis, transport of nutrients, and migration of blood cells across the barrier. A number of signaling pathways and molecules mediate endothelial permeability, which plays important roles in a variety of the physiological and pathological conditions. Fatty acid binding proteins (FABPs) are able to bind various hydrophobic molecules, such as long-chain fatty acids, prostaglandins and eicosanoids...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28441764/altered-adipogenesis-in-zebrafish-larvae-following-high-fat-diet-and-chemical-exposure-is-visualised-by-stimulated-raman-scattering-microscopy
#4
Marjo J den Broeder, Miriam J B Moester, Jorke H Kamstra, Peter H Cenijn, Valentina Davidoiu, Leonie M Kamminga, Freek Ariese, Johannes F de Boer, Juliette Legler
Early life stage exposure to environmental chemicals may play a role in obesity by altering adipogenesis; however, robust in vivo methods to quantify these effects are lacking. The goal of this study was to analyze the effects of developmental exposure to chemicals on adipogenesis in the zebrafish (Danio rerio). We used label-free Stimulated Raman Scattering (SRS) microscopy for the first time to image zebrafish adipogenesis at 15 days post fertilization (dpf) and compared standard feed conditions (StF) to a high fat diet (HFD) or high glucose diet (HGD)...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28394345/cripto-and-its-signaling-partner-grp78-drive-the-metastatic-phenotype-in-human-osteotropic-prostate-cancer
#5
E Zoni, L Chen, S Karkampouna, Z Granchi, E I Verhoef, F La Manna, J Kelber, R C M Pelger, M D Henry, E Snaar-Jagalska, G J L H van Leenders, L Beimers, P Kloen, P C Gray, G van der Pluijm, M Kruithof-de Julio
CRIPTO (CR-1, TDGF1) is a cell surface/secreted oncoprotein actively involved in development and cancer. Here, we report that high expression of CRIPTO correlates with poor survival in stratified risk groups of prostate cancer (PCa) patients. CRIPTO and its signaling partner glucose-regulated protein 78 (GRP78) are highly expressed in PCa metastases and display higher levels in the metastatic ALDH(high) sub-population of PC-3M-Pro4Luc2 PCa cells compared with non-metastatic ALDH(low). Coculture of the osteotropic PC-3M-Pro4Luc2 PCa cells with differentiated primary human osteoblasts induced CRIPTO and GRP78 expression in cancer cells and increases the size of the ALDH(high) sub-population...
April 10, 2017: Oncogene
https://www.readbyqxmd.com/read/28393070/assessment-of-toxicological-perturbations-and-variants-of-pancreatic-islet-development-in-the-zebrafish-model
#6
Karilyn E Sant, Haydee M Jacobs, Jiali Xu, Katrina A Borofski, Larry G Moss, Jennifer B Moss, Alicia R Timme-Laragy
The pancreatic islets, largely comprised of insulin-producing beta cells, play a critical role in endocrine signaling and glucose homeostasis. Because they have low levels of antioxidant defenses and a high perfusion rate, the endocrine islets may be a highly susceptible target tissue of chemical exposures. However, this endpoint, as well as the integrity of the surrounding exocrine pancreas, is often overlooked in studies of developmental toxicology. Disruption of development by toxicants can alter cell fate and migration, resulting in structural alterations that are difficult to detect in mammalian embryo systems, but that are easily observed in the zebrafish embryo model (Danio rerio)...
September 2016: Toxics
https://www.readbyqxmd.com/read/28391552/effect-of-empagliflozin-on-cardiac-biomarkers-in-a-zebrafish-model-of-heart-failure-clues-to-the-empa-reg-outcome-trial
#7
Xingjuan Shi, Subodh Verma, Junghwa Yun, Koroboshka Brand-Arzamendi, Krishna K Singh, Xiangdong Liu, Ankit Garg, Adrian Quan, Xiao-Yan Wen
The sodium-glucose cotransporter 2 (SGLT2) inhibitor empagliflozin was recently reported to reduce heart failure-associated hospitalizations and cardiovascular mortality amongst individuals with type 2 diabetes at high cardiovascular risk. We sought to elucidate the underlying mechanism(s) for these protective effects using a validated zebrafish heart failure model to evaluate the impact of empagliflozin on the expression of biomarkers of heart failure and mortality. We used aristolochic acid (AA) to induce heart failure in developing cmlc2::GFP transgenic zebrafish embryos and monitored BNP signaling in nppb::Luc transgenic zebrafish with a luciferase reporter assay...
April 8, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28386438/expression-and-function-of-atp-dependent-potassium-channels-in-zebrafish-islet-%C3%AE-cells
#8
Christopher H Emfinger, Alecia Welscher, Zihan Yan, Yixi Wang, Hannah Conway, Jennifer B Moss, Larry G Moss, Maria S Remedi, Colin G Nichols
ATP-sensitive potassium channels (KATP channels) are critical nutrient sensors in many mammalian tissues. In the pancreas, KATP channels are essential for coupling glucose metabolism to insulin secretion. While orthologous genes for many components of metabolism-secretion coupling in mammals are present in lower vertebrates, their expression, functionality and ultimate impact on body glucose homeostasis are unclear. In this paper, we demonstrate that zebrafish islet β-cells express functional KATP channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28327129/short-term-overfeeding-of-zebrafish-with-normal-or-high-fat-diet-as-a-model-for-the-development-of-metabolically-healthy-versus-unhealthy-obesity
#9
Kathrin Landgraf, Susanne Schuster, Andrej Meusel, Antje Garten, Thomas Riemer, Dorit Schleinitz, Wieland Kiess, Antje Körner
BACKGROUND: Obese individuals differ in their risk of developing metabolic and cardiovascular complications depending on fat distribution (subcutaneous versus visceral) and adipose tissue (AT) phenotype (hyperplasic versus hypertrophic). However, the exact mechanisms which determine whether an obese individual is metabolically healthy or unhealthy are not clear, and analyses of the underlying pathomechanisms are limited by the lack of suitable in vivo models in which metabolically healthy versus metabolically unhealthy AT accumulation can be specifically induced...
March 21, 2017: BMC Physiology
https://www.readbyqxmd.com/read/28284867/role-of-the-sugar-moiety-on-the-opioid-receptor-binding-and-conformation-of-a-series-of-enkephalin-neoglycopeptides
#10
Mònica Rosa, Verónica Gonzalez-Nunez, Katherine Barreto-Valer, Filipa Marcelo, Julia Sánchez-Sánchez, Luis P Calle, Juan C Arévalo, Raquel E Rodríguez, Jesús Jiménez-Barbero, Gemma Arsequell, Gregorio Valencia
Glycosylation by simple sugars is a drug discovery alternative that has been explored with varying success for enhancing the potency and bioavailability of opioid peptides. Long ago we described two O-glycosides having either β-Glucose and β-Galactose of (d-Met(2), Pro(5))-enkephalinamide showing one of the highest antinociceptive activities known. Here, we report the resynthesis of these two analogs and the preparation of three novel neoglycopeptide derivatives (α-Mannose, β-Lactose and β-Cellobiose)...
February 27, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28280764/lipid-quantitation-and-metabolomics-data-from-vitamin-e-deficient-and-sufficient-zebrafish-embryos-from-0-to-120-hours-post-fertilization
#11
Melissa McDougall, Jaewoo Choi, Hye-Kyeong Kim, Gerd Bobe, J Frederik Stevens, Enrique Cadenas, Robert Tanguay, Maret G Traber
The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28268202/adverse-effects-of-parental-zinc-deficiency-on-metal-homeostasis-and-embryonic-development-in-a-zebrafish-model
#12
Laura M Beaver, Yasmeen M Nkrumah-Elie, Lisa Truong, Carrie L Barton, Andrea L Knecht, Greg D Gonnerman, Carmen P Wong, Robert L Tanguay, Emily Ho
The high prevalence of zinc deficiency is a global public health concern, and suboptimal maternal zinc consumption has been associated with adverse effects ranging from impaired glucose tolerance to low birthweights. The mechanisms that contribute to altered development and poor health in zinc deficient offspring are not completely understood. To address this gap, we utilized the Danio rerio model and investigated the impact of dietary zinc deficiency on adults and their developing progeny. Zinc deficient adult fish were significantly smaller in size, and had decreases in learning and fitness...
May 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28217809/-generation-and-phenotype-analysis-of-zebrafish-mutations-of-obesity-related-genes-lepr-and-mc4r
#13
Fei Fei, Shao-Yang Sun, Yu-Xiao Yao, Xu Wang
Obesity has become a severe public health problem across the world, and seriously affects the health and life quality of human beings. Here we generated lepr and mc4r mutant zebrafish via the CRISPR/Cas9 technique, and performed morphological and functional characterizations of those mutants. We observed that there was no significant phenotypic difference between homozygous mutants and wild-type controls before 2.5 months post-fertilization (mpf). However, the adult lepr(-/-) and mc4r(-/-) individuals displayed increased food intake, heavier weight, and higher body fat percentage, the characteristics of obesity phenotypes...
February 25, 2017: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28184214/modeling-pancreatic-endocrine-cell-adaptation-and-diabetes-in-the-zebrafish
#14
REVIEW
Lisette A Maddison, Wenbiao Chen
Glucose homeostasis is an important element of energy balance and is conserved in organisms from fruit fly to mammals. Central to the control of circulating glucose levels in vertebrates are the endocrine cells of the pancreas, particularly the insulin-producing β-cells and the glucagon producing α-cells. A feature of α- and β-cells is their plasticity, an ability to adapt, in function and number as a response to physiological and pathophysiological conditions of increased hormone demand. The molecular mechanisms underlying these adaptive responses that maintain glucose homeostasis are incompletely defined...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28132765/zebrafish-akt2-is-essential-for-survival-growth-bone-development-and-glucose-homeostasis
#15
Dawei Zhang, Jing Wang, Chi Zhou, Wuhan Xiao
As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular processes in mammals. However, its role and underlying mechanisms in zebrafish remain largely unknown. To elucidate the function of akt2 in zebrafish, we generated zebrafish lacking akt2 gene via CRISPR/Cas9 technology. Akt2-null zebrafish exhibit partial lethality and severe growth deficiency, which is different from those observed in akt2-null mice. Furthermore, akt2-null zebrafish display deficiency in fin ray development, but their cartilage is not affected...
January 26, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28129847/analysis-of-pancreatic-disease-in-zebrafish
#16
S C Eames Nalle, K F Franse, M D Kinkel
One of the appeals of the zebrafish model is the relative ease of studying disease progression from embryonic or larval stages through to adulthood. Because of this, the zebrafish has become an important model for postembryonic pancreatic disease, particularly diabetes and pancreatic cancer. Here we present methods for using the adult zebrafish to analyze pancreas function and structure, with an emphasis on the endocrine pancreas and the beta cells. The methods include fasting, weighing adults, and anesthetizing adults, and intraperitoneal injection of glucose based on body weight...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28123325/metabolite-profiles-of-ginsenosides-rk1-and-rg5-in-zebrafish-using-ultraperformance-liquid-chromatography-quadrupole-time-of-flight-ms
#17
Wenwen Shen, Yingjie Wei, Daoquan Tang, Xiaobin Jia, Bin Chen
BACKGROUND: In the present study, metabolite profiles of ginsenosides Rk1 and Rg5 from red ginseng or red notoginseng in zebrafish were qualitatively analyzed with ultraperformance liquid chromatography/quadrupole-time-of-flight MS, and the possible metabolic were pathways proposed. METHODS: After exposing to zebrafish for 24 h, we determined the metabolites of ginsenosides Rk1 and Rg5. The chromatography was accomplished on UPLC BEH C18 column using a binary gradient elution of 0...
January 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28102299/systemic-regulation-of-l-carnitine-in-nutritional-metabolism-in-zebrafish-danio-rerio
#18
Jia-Min Li, Ling-Yu Li, Xun Qin, Li-Jun Ning, Dong-Liang Lu, Dong-Liang Li, Mei-Ling Zhang, Xin Wang, Zhen-Yu Du
Excess fat accumulation has been observed widely in farmed fish; therefore, efficient lipid-lowering factors have obtained high attention in the current fish nutrition studies. Dietary L-carnitine can increase fatty acid β-oxidation in mammals, but has produced contradictory results in different fish species. To date, the mechanisms of metabolic regulation of L-carnitine in fish have not been fully determined. The present study used zebrafish to investigate the systemic regulation of nutrient metabolism by dietary L-carnitine supplementation...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28095320/lethal-dysregulation-of-energy-metabolism-during-embryonic-vitamin-e-deficiency
#19
Melissa McDougall, Jaewoo Choi, Hye-Kyeong Kim, Gerd Bobe, J Frederik Stevens, Enrique Cadenas, Robert Tanguay, Maret G Traber
Vitamin E (α-tocopherol, VitE) was discovered in 1922 for its role in preventing embryonic mortality. We investigated the underlying mechanisms causing lethality using targeted metabolomics analyses of zebrafish VitE-deficient embryos over five days of development, which coincided with their increased morbidity and mortality. VitE deficiency resulted in peroxidation of docosahexaenoic acid (DHA), depleting DHA-containing phospholipids, especially phosphatidylcholine, which also caused choline depletion. This increased lipid peroxidation also increased NADPH oxidation, which depleted glucose by shunting it to the pentose phosphate pathway...
March 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28005350/responses-of-the-proteome-and-metabolome-in-livers-of-zebrafish-exposed-chronically-to-environmentally-relevant-concentrations-of-microcystin-lr
#20
Liang Chen, Yufei Hu, Jun He, Jun Chen, John P Giesy, Ping Xie
In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish [Formula: see text] (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 μg MC-LR/L for 90 days, and iTRAQ-based proteomics and (1)H NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 μg MC-LR/L...
January 3, 2017: Environmental Science & Technology
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