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https://www.readbyqxmd.com/read/29935277/impairment-of-spatial-working-memory-and-oxidative-stress-induced-by-repeated-crack-cocaine-inhalation-in-rats
#1
Ingryd Fortes Souza Lipaus, Elisa Fraga Gomes, Cleciane Waldetário Martins, Cristina Martins E Silva, Rita Gomes Wanderley Pires, Fernanda Malgarin, Patrícia Fernanda Schuck, Ester Miyuki Nakamura Palacios, Lívia Carla de Melo Rodrigues
Crack cocaine is a highly toxic drug with great potential to induce addiction. It produces more intense effects than cocaine powder, with its use having grown worldwide. However, few studies have focused on the cognitive and biochemical consequences that result from crack cocaine inhalation. This study examined the effects of direct crack cocaine inhalation on spatial working memory and brain oxidative stress parameters in rats. Male adult Wistar rats, well-trained in an eight-arm radial maze (8-RM), underwent five sessions of crack cocaine inhalation (crack cocaine group) once a day or inhalation simulation (sham group), being tested in 1-h delayed tasks 24 h after the last inhalation...
June 20, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29935098/identifying-the-best-body-weight-status-index-associated-with-metabolic-risk-in-youth
#2
Thayse Natacha Gomes, Alan Nevill, Peter T Katzmarzyk, Sara Pereira, Marcos Moura Dos Santos, Rojapon Buranarugsa, Fernanda Dos Santos, Michele Souza, Raquel Chaves, José Maia
This study investigated the association of six different anthropometric markers with metabolic syndrome to find the most suited to predict children at risk. Sample comprises 1324 Portuguese youth (701 girls, 623 boys), aged 10-17 years. Six anthropometric markers were included: body mass index (BMI), BMI z-score, tri-ponderal index (TPI), waist circumference (WC), WC/height ratio (WC/H), and WC/H adjusted ratio (WC/Hadj ). A standardized metabolic risk score (zMR) was computed by summing of standardized values for fasting glucose, triglycerides, high density lipoprotein cholesterol, and mean arterial blood pressure...
June 23, 2018: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29934882/molecular-and-immnune-diagnosis-further-testing-for-human-strongyloidiasis
#3
Larissa R Bosqui, Priscilla D Marques, Gessica B de Melo, Maria do Rosário F Gonçalves-Pires, Fernanda M Malta, Wander R Pavanelli, Ivete Conchon-Costa, Julia M Costa-Cruz, Fabiana M Paula, Idessania N Costa
INTRODUCTION: Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. OBJECTIVE: Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. METHODS: Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests...
June 21, 2018: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/29934712/subacute-exposure-to-residual-oil-fly-ash-rofa-increases-ehsp70-content-and-extracellular-to-intracellular-hsp70-ratio-a-relation-with-oxidative-stress-markers
#4
Fernanda Giesel Baldissera, Analú Bender Dos Santos, Maicon Machado Sulzbacher, Pauline Brendler Goettems-Fiorin, Matias Nunes Frizzo, Mirna Stela Ludwig, Claudia Ramos Rhoden, Thiago Gomes Heck
The purpose of this study was to evaluate whether exposure to particles induces an imbalance in 70-kDa heat shock proteins (HSP70). Since intracellularly (iHSP70) it has anti-inflammatory roles whereas extracellularly (eHSP70) it has pro-inflammatory roles, we evaluate the effect of residual oil fly ash (ROFA) exposure on eHSP70-to-iHSP70 ratio (H index), a biomarker of inflammatory status that is related to oxidative stress in plasma and lymphoid tissue. Wistar rats that received ROFA suspension for three consecutive days (750 μg) showed an increase in plasma eHSP70 levels (mainly the 72-kDa inducible form)...
June 22, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29934500/cysteine-allows-ovarian-cancer-cells-to-adapt-to-hypoxia-and-to-escape-from-carboplatin-cytotoxicity
#5
Sofia C Nunes, Cristiano Ramos, Filipa Lopes-Coelho, Catarina O Sequeira, Fernanda Silva, Sofia Gouveia-Fernandes, Armanda Rodrigues, António Guimarães, Margarida Silveira, Sofia Abreu, Vítor E Santo, Catarina Brito, Ana Félix, Sofia A Pereira, Jacinta Serpa
Ovarian cancer is the second most common gynaecologic malignancy and the main cause of death from gynaecologic cancer, due to late diagnosis and chemoresistance. Studies have reported the role of cysteine in cancer, by contributing for hydrogen sulphide (H2 S) generation and as a precursor of glutathione (GSH). However, the role of cysteine in the adaptation to hypoxia and therapy response remains unclear. We used several ovarian cancer cell lines, ES2, OVCAR3, OVCAR8, A2780 and A2780cisR, to clarify cysteine relevance in ovarian cancer cells survival upon hypoxia and carboplatin...
June 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29934256/role-of-self-expanding-stents-in-the-treatment-of-intrathoracic-dehiscence-after-ivor-lewis-esophagectomy
#6
David Ruiz de Angulo, María Ángeles Ortiz, Vicente Munitiz, Luisa Fernanda Martínez de Haro, Fernando Alberca, Andrés Serrano, Juan Egea, Pascual Parrilla
INTRODUCTION: The role that self-expanding stents play in the treatment of dehiscence after transthoracic esophagectomy is not well defined and controversial. Our aim is to describe the experience in a tertiary care hospital using these devices for treating dehiscence after Ivor Lewis esophagectomy. METHODS: Descriptive observational study of patients who suffered anastomotic dehiscence after a transthoracic esophagectomy, and especially those treated with stents, in the period between 2011-2016 at our hospital...
June 19, 2018: Cirugía Española
https://www.readbyqxmd.com/read/29933023/effects-of-static-magnetic-fields-on-natural-or-magnetized-mesenchymal-stromal-cells-repercussions-for-magnetic-targeting
#7
Luisa H A Silva, Sueli M da Silva, Emilia C D Lima, Renata C Silva, Daniel J Weiss, Marcelo M Morales, Fernanda F Cruz, Patricia R M Rocco
The magnetic targeting (MT) technique improves delivery of mesenchymal stromal cells (MSCs) to target sites. However, the moderate-intensity static magnetic fields (SMF) used for MT may exert adverse effects on MSCs. Thus, we aimed to evaluate the effects of SMF on MSCs in vitro. Cells were initially magnetized using citrate-coated magnetite nanoparticles. Then, control and magnetized MSCs were transferred to an in vitro MT system and exposed to 0.3-0.45 Tesla SMFs. MSC viability, morphology, ultrastructure, proliferation rates, differentiation, and immunomodulation were evaluated after 24 and 48hours of exposure...
June 19, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29932988/inhibition-of-the-anxa1-fpr1-autocrine-axis-reduces-mda-mb-231-breast-cancer-cell-growth-and-aggressiveness-in-vitro-and-in-vivo
#8
Lara Vecchi, Mariana Alves Pereira Zoia, Tiago Goss Santos, Adriano de Oliveira Beserra, Cristiano Manuel Colaço Ramos, Bruna França Matias Colombo, Yara Cristina Paiva Maia, Victor Piana de Andrade, Sara Teixeira Soares Mota, Thaise Gonçalves de Araújo, Fernanda Van Petten de Vasconcelos Azevedo, Fernando Augusto Soares, Sonia Maria Oliani, Luiz Ricardo Goulart
Breast Cancer (BC) is a highly heterogeneous disease whose most aggressive behavior is displayed by triple-negative breast cancer (TNBC), which lacks an efficient targeted therapy. Despite its controversial role, one of the proteins that having been linked with BC is Annexin A1 (AnxA1), which is a Ca+2 binding protein that acts modulating the immune system, cell membrane organization and vesicular trafficking. In this work we analyzed tissue microarrays of BC samples and observed a higher expression of AnxA1 in TNBCs and in lymph node metastasis...
June 19, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29932894/fructose-1-6-bisphosphate-preserves-glucose-metabolism-integrity-and-reduces-reactive-oxygen-species-in-the-brain-during-experimental-sepsis
#9
Anderson V Catarina, Carolina Luft, Samuel Greggio, Gianina T Venturin, Fernanda Ferreira, Eduardo P Marques, Letícia Rodrigues, Krista Wartchow, Marina C Leite, Carlos A Gonçalves, Angela T S Wyse, Jaderson C Da Costa, Jarbas R De Oliveira, Gisele Branchini, Fernanda B Nunes
Sepsis is one of the main causes of hospitalization and mortality in Intensive Care Units. One of the first manifestations of sepsis is encephalopathy, reported in up to 70% of patients, being associated with higher mortality and morbidity. The factors that cause sepsis-associated encephalopathy (SAE) are still not well known, and may be multifactorial, as perfusion changes, neuroinflammation, oxidative stress and glycolytic metabolism alterations. Fructose-1,6-bisphosphate (FBP), a metabolite of the glycolytic route, has been reported as neuroprotective agent...
June 19, 2018: Brain Research
https://www.readbyqxmd.com/read/29932819/biochemical-characterization-and-evaluation-of-invertases-produced-from-saccharomyces-cerevisiae-cat-1-and-rhodotorula-mucilaginosa-for-the-production-of-fructooligosaccharides
#10
Paula Mirella Gomes Barbosa, Tobias Pereira de Morais, Cinthia Aparecida de Andrade Silva, Flávia Regina da Silva Santos, Nayara Fernanda Lisbo Garcia, G G Fonseca, Rodrigo Simões Ribeiro Leite, Marcelo Fossa da Paz
Invertases are used for several purposes; one among these is the production of fructooligosaccharides. The aim of this study was to biochemically characterize invertase from industrial Saccharomyces cerevisiae CAT-1 and Rhodotorula mucilaginosa isolated from Cerrado soil. The optimum pH and temperature were 4.0 and 70 °C for Rhodotorula mucilaginosa invertase and 4.5 and 50 °C for Saccharomyces cerevisiae invertase. The pH and thermal stability from 3.0 to 10.5 and 75 °C for R. mucilaginosa invertase, respectively...
June 22, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29932491/intake-of-protein-hydrolysates-and-phenolic-fractions-isolated-from-flaxseed-ameliorates-tnbs-induced-colitis
#11
Fernanda G D E Silva, Lisiery N Paiatto, Aureo T Yamada, Flavia M Netto, Patrícia U Simioni, Wirla M S C Tamashiro
SCOPE: In an attempt to develop new therapeutic treatments for colitis, we evaluated the effects of phenolic compounds and protein hydrolysates fractions of flaxseed on the in vitro production of pro-inflammatory mediators created by macrophages and on the course of experimental colitis. METHODS AND RESULTS: The anti-inflammatory effects of phenolic compounds (Phi) and protein hydrolysates (phr-FPH) from flaxseeds were studied in RAW264.7 cells and in TNBS colitis model...
June 22, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29931604/dietary-supplementation-for-santa-in%C3%A3%C2%AAs-hair-ewes-on-pasture-at-pre-and-postpartum-periods-dry-matter-intake-digestibility-milk-production-and-mineral-metabolism
#12
Sheila Vilarindo de Sousa, Marcos Jácome de Araújo, Tairon Panunzio Dias E Silva, Carlo Aldrovandi Torreão Marques, Jacira Neves da Costa Torreão, Leilson Rocha Bezerra, Isak Samir de Sousa Lima, Fernanda Patrícia Gottardi
The aim of this study was to evaluate the impact of different levels of dietary supplementation and reproductive stages on dry matter intake, digestibility, milk production, and mineral metabolism in Santa Inês hair ewes. Two dietary supplement levels of 0.5 and 1.5%, based on body weight, were used. A total of 12 hair ewes (six subjected to 0.5 and six subjected to 1.5% of concentrate supplementation based on body weight-BW) of the Santa Inês breed were evaluated in a completely randomized design with fixed effects of supplementation level, period, and its interactions...
June 22, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29931152/early-application-of-microsurgical-flaps-in-the-electric-burns-of-extremities-a-two-institutional-case-series
#13
Júlio C Castro, Pedro S Coltro, Lincoln S Millan, Fernanda B Corrêa, Jayme A Farina Junior
Some groups have avoided early microsurgical flaps in electrical burns under the pretext of injury to the microvasculature, which could increase vascular thrombosis. However, this option frequently results in amputation of the extremity. This study aims to evaluate the early application of microsurgical flaps for the treatment of electrical burns of extremities. A case series was retrospectively evaluated including patients with electrical trauma in extremities undergoing early microsurgical reconstruction...
December 4, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/29930680/compara%C3%A3-%C3%A3-o-entre-cirurgia-aberta-e-endovascular-no-tratamento-do-aneurisma-da-art%C3%A3-ria-popl%C3%A3-tea-uma-revis%C3%A3-o
#14
REVIEW
Ana Fernanda Fagundes Gonçalves, Carlos Augusto Pelek, Lorena Slusarz Nogueira, Renan Francisco de Carvalho, Matheo Augusto Morandi Stumpf, Ricardo Zanetti Gomes, Ana Claudia Garabeli Cavalli Kluthcovsky
No abstract text is available yet for this article.
January 2018: Jornal Vascular Brasileiro
https://www.readbyqxmd.com/read/29930648/adolescentes-comportamento-e-risco-cardiovascular
#15
Ivelise Fhrideraid Alves Furtado da Costa, Carla Campos Muniz Medeiros, Fernanda Dayenne Alves Furtado da Costa, Camilla Ribeiro Lima de Farias, Diogo Rodrigues Souza, Wellington Sabino Adriano, Mônica Oliveira da Silva Simões, Danielle Franklin Carvalho
No abstract text is available yet for this article.
July 2017: Jornal Vascular Brasileiro
https://www.readbyqxmd.com/read/29930250/author-correction-omega-3-docosahexaenoic-acid-induces-pyroptosis-cell-death-in-triple-negative-breast-cancer-cells
#16
Nathalia Pizato, Beatriz Christina Luzete, Larissa Fernanda Melo Vasconcelos Kiffer, Luís Henrique Corrêa, Igor de Oliveira Santos, José Antônio Fagundes Assumpção, Marina Kiyomi Ito, Kelly Grace Magalhães
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
June 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29930047/genome-sequence-of-a-staphylococcus-epidermidis-strain-gth12-associated-with-candida-albicans-sc5314-cultured-under-hypoxia-at-37%C3%A2-c-in-glycerol-for-12-weeks
#17
Danielle do Carmo Ferreira Bruno, Thais Fernanda Bartelli, Marcelo R S Briones
Polymicrobial infections with mixed-species biofilms are important health problems because of increased antimicrobial resistance and worse patient outcomes than with monomicrobial infections. Here, we present the whole-genome sequence of Staphylococcus epidermidis strain GTH12, which was cocultured with the yeast Candida albicans SC5314 (generating C. albicans strain SC5314 GTH12), thus providing genomic information on polymicrobial infections.
June 21, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29929845/niobium-addition-to-sol-gel-derived-bioactive-glass-powders-and-scaffolds-in-vitro-characterization-and-effect-on-pre-osteoblastic-cell-behavior
#18
Gabriela de Souza Balbinot, Fabricio Mezzomo Collares, Fernanda Visioli, Priscilla Barbosa Ferreira Soares, Antonio Shigueaki Takimi, Susana Maria Werner Samuel, Vicente Castelo Braco Leitune
No abstract text is available yet for this article.
June 18, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29928182/a-novel-mgp-gene-mutation-causing-keutel-syndrome-in-a-brazilian-patient
#19
Eduardo Perrone, Kelin Chen, Marco Ramos, Maria Fernanda Milanezi, Viviane Nakano, Ariane Falconi, Juliana Silva, Jamille Campos, Celia M C Silva, Joao B O Filho, Ana B A Perez
Keutel syndrome is caused by mutations in the matrix gamma-carboxyglutamic acid ( MGP ) gene (OMIM 154870) and is inherited in an autosomal recessive fashion. It is characterized by brachydactyly, pulmonary artery stenosis, a distinctive facial phenotype, and cartilage calcification. To date, only 36 cases have been reported worldwide. We describe clinical and molecular findings of the first Brazilian patient with Keutel syndrome. Keutel syndrome was suspected based on clinical and morphological evaluation, so we sequenced the MGP gene using the TruSight One Sequencing Panel (Illumina)...
May 2018: Molecular Syndromology
https://www.readbyqxmd.com/read/29928016/in-silico-and-in-vitro-analysis-of-boap3d1-protein-interaction-with-bovine-leukaemia-virus-gp51
#20
Adriana Patricia Corredor, Janneth González, Luis Alfredo Baquero, Hernando Curtidor, Nury Nathalia Olaya-Galán, Manuel Alfonso Patarroyo, María Fernanda Gutiérrez
The envelope glycoprotein 51 (gp51) is essential for bovine leukaemia virus (BLV) entry to bovine B-lymphocytes. Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools...
2018: PloS One
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