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Yu Zhao, Hongqi Zhang
The effects of propofol vs. sevoflurane combined with remifentanil on the pain index, inflammatory factors and postoperative cognitive function in spine fracture patients were studied and analyzed. A total of 62 patients with vertebral fracture were randomly divided into the propofol group (P group, n=41) and the sevoflurane group (S group, n=41). P group used induction anesthesia with propofol, and maintained anesthesia via intravenous injection of remifentanil. While patients in S group received induction anesthesia with sevoflurane, and also remifentanil as the maintained anesthesia...
April 2018: Experimental and Therapeutic Medicine
Jacob Rozmus, Sabine Ivison, Amina Kariminia, Vivian M Leung, Susanna Sung, Peter Subrt, Stephanie J Lee, Eric Boilard, Irwin Walker, Ronan Foley, Jeff Lipton, Geneviève Gallagher, Stephen Couban, Kirk R Schultz
Toll-like receptor-9 (TLR9) responsive B cells have previously been associated with the onset of extensive chronic graft-versus-host disease (cGvHD). We hypothesized that the onset of cGvHD associated with a higher level of plasma-free mitochondrial DNA (mtDNA), a putative TLR9 agonist. Plasma cell-free mtDNA levels were measured in 39 adult patients post-HSCT with and without cGvHD. mtDNA was isolated from plasma and quantified by Q-PCR amplification. We correlated B cell responsiveness to CpG-DNA, a prototypical TLR9 agonist, and previously identified cGVHD biomarkers with mtDNA levels...
March 21, 2018: Bone Marrow Transplantation
Jian-Wei Lou, Liang-Liang Cao, Qiao Zhang, Dong-Jing Jiang, Wei-Feng Yao, Bei-Hua Bao, Yu-Dan Cao, Yu-Ping Tang, Li Zhang, Kun Wang, Guan-Cheng Dai
ETHNOPHARMACOLOGICAL RELEVANCE: Kansui, the root of Euphorbia kansui S.L.Liou ex S.B.Ho (E.kansui), is a classical traditional Chinese medicine (TCM) with certain toxicity. According to the theory of TCM, kansui fry-baked with vinegar (VEK) possesses low toxicity and mild diuretic and purgative efficacy. In clinical practice, it is commonly used for the treatment of ascites and oliguria. The present study aimed to evaluate the toxicity and efficacy of different fractions of VEK and reveal the underlying material basis by employing an animal model of malignant ascites effusion (MAE) in rats...
March 17, 2018: Journal of Ethnopharmacology
Fei Yan, Yu Sun, Yang Mao, Meiying Wu, Zhiting Deng, Shuai Li, Xin Liu, Li Xue, Hairong Zheng
Cardiovascular diseases resulting from atherosclerosis have become a serious threat to human health. It is well-known that an ongoing inflammatory response is involved during atherosclerosis progression that ultimately results in the accumulation of lipids and formation of plaques. Monitoring the pathological changes during the inflammatory response will be of great significance for early diagnosis and therapeutic evaluation of atherosclerosis. Targeted contrast-enhanced ultrasonography has been shown to be a promising noninvasive imaging technique for evaluating the degree of atherosclerosis and may potentially be translated to clinical imaging in the future...
2018: Theranostics
Pallavi Gurung, Suhrid Banskota, Nikita Katila, Jaya Gautam, Tara Man Kadayat, Dong-Young Choi, Eung Seok Lee, Tae Cheon Jeong, Jung-Ae Kim
The pathogenesis of inflammatory bowel disease (IBD) is associated with production of immense pro-inflammatory cytokines including TNF-α. Once generated, TNF-α stimulates production of various pro-inflammatory cytokines and disrupts mucosal barrier by inducing inflamed mucosal epithelial cell death. In the present study, we investigated inhibitory effects of TI-1-162, a hydroxyindenone derivative, against TNF-α-induced and TNBS-induced colon inflammation. TI-1-162 showed inhibitory effect on the TNF-α-induced adhesion of U937 monocytic cells to HT-29 colonic epithelial cells (IC50 = 0...
March 16, 2018: European Journal of Pharmacology
C Incorvaia, S Masieri, C Cavaliere, E Makri, B Sposato, F Frati
A large amount of data show that AR and asthma are associated both epidemiologically and clinically, introducing the definition of “united airway disease”. The mechanisms underlying such association were initially suggested to start from the nose, including the loss of the protective and homeostatic effects of nasal function, the activation of a naso-bronchial reflex and the spread of allergic inflammation from the nose to the lower airways. Later, other factors such as microbial stimuli and systemic inflammatory mechanisms, involving bloodstream and bone marrow, were advocated...
January 2018: Journal of Biological Regulators and Homeostatic Agents
M I Maiorino, G Bellastella, D Giugliano, K Esposito
Metabolic diseases are associated with chronic low-grade inflammation, which has been indicated as a potential mediator of endothelial dysfunction and cardiovascular disease. Visceral adiposity is thought to be the starting condition of the inflammatory state through the release of inflammatory cytokines, including TNF-alpha, CRP, and IL-6, which in turn promote endothelial dysfunction, endothelial expression of chemokines (IL-1) and adhesion molecules (ICAM-1, VCAM-1, and P-selectin), and the inhibition of anti-atherogenic factors (adiponectin)...
March 16, 2018: Journal of Endocrinological Investigation
Nicholas Beazley-Long, Catherine Elizabeth Moss, William Robert Ashby, Samuel Marcus Bestall, Fatimah Almahasneh, Alexandra Margaret Durrant, Andrew Vaughan Benest, Zoe Blackley, Kurt Ballmer-Hofer, Masanori Hirashima, Richard Phillip Hulse, David Owen Bates, Lucy Frances Donaldson
Chronic pain can develop in response to conditions such as inflammatory arthritis. The central mechanisms underlying the development and maintenance of chronic pain in humans are not well elucidated although there is evidence for a role of microglia and astrocytes. However in pre-clinical models of pain, including models of inflammatory arthritis, there is a wealth of evidence indicating roles for pathological glial reactivity within the CNS. In the spinal dorsal horn of rats with painful inflammatory arthritis we found both a significant increase in CD11b+ microglia-like cells and GFAP+ astrocytes associated with blood vessels, and the number of activated blood vessels expressing the adhesion molecule ICAM-1, indicating potential glio-vascular activation...
March 13, 2018: Brain, Behavior, and Immunity
Kirtikar Shukla, Himangshu Sonowal, Ashish Saxena, Kota V Ramana
Although didymin, a flavonoid-O-glycosides compound naturally found in the citrus fruits, has been reported to be a potent anticancer agent in the prevention of various cancers, its role in the prevention of cardiovascular complications is unclear. Most importantly, its effect in the prevention of endothelial dysfunction, a pathological process involved in the atherogenesis, is unknown. We have examined the efficacy of didymin in preventing the high glucose (HG; 25mM)-induced human umbilical vein endothelial cells (HUVECs) dysfunction...
March 13, 2018: Biochemical Pharmacology
Qingdong Guan, Yun Li, Tanner Shpiruk, Swaroop Bhagwat, Donna A Wall
AIM: Establishment of a potency assay in the manufacturing of clinical-grade mesenchymal stromal cells (MSCs) has been a challenge due to issues of relevance to function, timeline and variability of responder cells. In this study, we attempted to develop a potency assay for MSCs. METHODS: Clinical-grade bone marrow-derived MSCs were manufactured. The phenotype and immunosuppressive functions of the MSCs were evaluated based on the International Society for Cellular Therapy guidelines...
March 13, 2018: Cytotherapy
Lei Zhang, Jie Ren, Peidian Shi, Dong Lu, Chengxue Zhao, Yanxin Su, Lilin Zhang, Jinhai Huang
B4GALT5, also known as β-1, 4 galactosyltransferase V, is one of the members of β-1, 4 galactosyltransferase gene (B4GALT) family, which was concerned with embryonic development, tumor generation, other malignant diseases. In this study, we firstly cloned porcine B4GALT (pB4GALT5) from porcine alveolar macrophages, and predicted the structural domain and function of seven porcine β-1, 4 galactosyltransferase (I-VII) based on transcriptome analysis of PRRSV infected cells. Additionally, the upregulated porcine B4GALT5 expression was detected from PRRSV infected porcine alveolar macrophage (PAM) cells...
2018: Frontiers in Cellular and Infection Microbiology
Nicolas S Merle, Anne Grunenwald, Marie-Lucile Figueres, Sophie Chauvet, Marie Daugan, Samantha Knockaert, Tania Robe-Rybkine, Remi Noe, Olivia May, Marie Frimat, Nathan Brinkman, Thomas Gentinetta, Sylvia Miescher, Pascal Houillier, Veronique Legros, Florence Gonnet, Olivier P Blanc-Brude, Marion Rabant, Regis Daniel, Jordan D Dimitrov, Lubka T Roumenina
Intravascular erythrocyte destruction, accompanied by the release of pro-oxidative and pro-inflammatory components hemoglobin and heme, is a common event in the pathogenesis of numerous diseases with heterogeneous etiology and clinical features. A frequent adverse effect related to massive hemolysis is the renal injury and inflammation. Nevertheless, it is still unclear whether heme--a danger-associated molecular pattern--and ligand for TLR4 or upstream hemolysis-derived products are responsible for these effects...
2018: Frontiers in Immunology
Antoine Abou Jaoude, Lise Badiqué, Mohamed Mlih, Sara Awan, Sunning Guo, Alexandre Lemle, Clauda Abboud, Sophie Foppolo, Lionel Host, Jérôme Terrand, Hélène Justiniano, Joachim Herz, Rachel L Matz, Philippe Boucher
ShcA is an adaptor protein that binds to the cytoplasmic tail of receptor tyrosine kinases and of the Low Density Lipoprotein-related receptor 1 (LRP1), a trans-membrane receptor that protects against atherosclerosis. Here, we examined the role of endothelial ShcA in atherosclerotic lesion formation. We found that atherosclerosis progression was markedly attenuated in mice deleted for ShcA in endothelial cells, that macrophage content was reduced at the sites of lesions, and that adhesion molecules such as the intercellular adhesion molecule-1 (ICAM-1) were severely reduced...
March 14, 2018: Scientific Reports
Huisheng Wu, Chaoliang Tang, Lydia Wai Tai, Weifeng Yao, Peipei Guo, Junmou Hong, Xin Yang, Xinyi Li, Zhao Jin, Jianjuan Ke, Yanlin Wang
Background & Aims:  Ischemic stroke has been ranked the second cause of death in patients worldwide.Inflammation which is activated during cerebral ischemia/reperfusion is an important mechanism leading to brain injury. This study aimed to investigate the effect of flurbiprofen axetil on cerebral I/R injury and the role of inflammation in this process. Methods:  Rats were subjected to sham operation or global cerebral I/R with or without flurbiprofen axetil (5 mg/kg or 10 mg/kg). Global cerebral ischemia was achieved by occlusion of bilateral common carotid arteries combined with hypotension for 20 min followed by reperfusion for 72 h...
March 14, 2018: Bioscience Reports
Miho Ibi, Sawa Horie, Seiko Kyakumoto, Naoyuki Chosa, Mariko Yoshida, Masaharu Kamo, Masato Ohtsuka, Akira Ishisaki
Many inflammatory cells are known to home to inflamed temporomandibular joint (TMJ) tissues by stimulation with cytokines and chemokines produced by inflammatory lesions in the TMJ. However, how the inflammatory cells affect the progression of inflammation in TMJ synovial tissues after their homing to inflamed TMJ site is still uncertain. Here, we isolated and cultured TMJ synoviocyte-like cells (TMJSCs) from murine TMJ tissues. We demonstrated that interleukin 1b (IL-1β) upregulated expression of monocyte chemoattractant protein 1 (MCP-1) in TMJSCs...
March 13, 2018: Bioscience Reports
Kung-Yen Chen, Jui-An Lin, Han-Yun Yao, An-Chih Hsu, Yu-Ting Tai, Jui-Tai Chen, Mao-Chih Hsieh, Tang-Long Shen, Ren-Yi Hsu, Hong-Tan Wu, Guey Horng Wang, Bing-Ying Ho, Yu-Pei Chen
Arctigenin (ATG), a lignin extracted from Arctium lappa (L.), exerts antioxidant and anti-inflammatory effects. We hypothesized that ATG exerts a protective effect on hepatocytes by preventing nonalcoholic fatty liver disease (NAFLD) progression associated with lipid oxidation-associated lipotoxicity and inflammation. We established an in vitro NAFLD cell model by using normal WRL68 hepatocytes to investigate oleic acid (OA) accumulation and the potential bioactive role of ATG. The results revealed that ATG inhibited OA-induced lipid accumulation, lipid peroxidation, and inflammation in WRL68 hepatocytes, as determined using Oil Red O staining, thiobarbituric acid reactive substance assay, and inflammation antibody array assays...
February 11, 2018: Nutrition Research
Xianyao Li, Yaqin Tang, Binbin Ma, Zheng Wang, Jinying Jiang, Shengjie Hou, Shuhang Wang, Jie Zhang, Meichun Deng, Zhigui Duan, Xing Tang, Alex F Chen, Liping Jiang
OBJECTIVE: The peptide lycosin-I has anti-bacterial and anti-cancer capacities. However, the anti-inflammatory activity of lycosin-I remains unknown. We investigated whether lycosin-I could attenuate inflammation. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with lycosin-I before exposure to tumor necrosis factor-α (TNF-α). The expression of intercellular cell adhesion molecule-1 (ICAM-1), nuclear transcription factor-kappa B (NF-κB) p65 and inhibitory subunit of NF-κB alpha (IκBα) was evaluated by western blot...
March 10, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Mónica Muñoz-Vega, Felipe Massó, Araceli Páez, Elizabeth Carreón-Torres, Hector A Cabrera-Fuentes, José Manuel Fragoso, Nonanzit Pérez-Hernández, Laurent O Martinez, Souad Najib, Gilberto Vargas-Alarcón, Óscar Pérez-Méndez
BACKGROUND: Primary cultures endothelial cells have been used as models of endothelial related diseases such atherosclerosis. Biological behavior of primary cultures is donor-dependent and data could not be easily reproducible; endothelial cell lines are emerging options, particularly, human dermal microvascular endothelial cells (HMEC-1), that should be validated to substitute primary cultures for the study of HDL functions. METHODS: Morphology, size and granularity of cells were assessed by phase contrast microscopy and flow cytometry of HMEC-1...
March 9, 2018: Lipids in Health and Disease
Roxane J Hillier, Elvis Ojaimi, David T Wong, Michael Y K Mak, Alan R Berger, Radha P Kohly, Peter J Kertes, Farzin Forooghian, Shelley R Boyd, Kenneth Eng, Filiberto Altomare, Louis R Giavedoni, Rosane Nisenbaum, Rajeev H Muni
Importance: Variability in response to anti-vascular endothelial growth factor (VEGF) treatment in diabetic macular edema (DME) remains a significant clinical challenge. Biomarkers could help anticipate responses to anti-VEGF therapy. Objectives: To investigate aqueous humor cytokine level changes in response to intravitreal ranibizumab therapy for the management of DME, and to determine the association between baseline aqueous levels and anatomic response. Design, Setting, and Participants: In this prospective multicenter cohort study, 49 participants with diabetes mellitus complicated by center-involving DME, with a central subfield thickness of 310 μm or greater on spectral-domain optical coherence tomography (SD-OCT), were recruited from December 22, 2011, to June 13, 2013 and statistical analysis were performed from March 1, 2017, to June 1, 2017...
March 8, 2018: JAMA Ophthalmology
F Santamaria-Martos, I Benítez, C Girón, F Barbé, M A Martínez-García, L Hernández, J M Montserrat, E Nagore, A Martorell-Calatayud, F Campos-Rodriguez, J Corral, V Cabriada, J Abad, O Mediano, M F Troncoso, I Cano-Pumarega, A M Fortuna Gutierrez, T Diaz-Cambriles, M Somoza-Gonzalez, I Almendros, R Farre, D Gozal, M Sánchez-de-la-Torre
The goal of this study was to assess the relationship between the severity of OSA and the levels of carcinogenesis- and tumour growth-related biomarkers, in patients with cutaneous melanoma (CM).This multicentre observational study included patients who were newly diagnosed with melanoma. The patients were classified as non-OSA (apnoea-hypopnoea index (AHI); 0-5 events/h), mild-OSA (AHI; 5-15 events/h), and moderate to severe OSA (AHI>15 events/h). Enzyme-linked immunosorbent assays (ELISAs) were performed to analyse the serum levels of hypoxia- and tumour adhesion-related biomarkers (VEGF, IL8, ICAM-1, VCAM-1), and markers of tumour aggressiveness (S100B, MIA)...
March 9, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
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