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Streptococcus mutans

Stefanie Amend, Roland Frankenberger, Susanne Lücker, Eugen Domann, Norbert Krämer
OBJECTIVES: The present study aimed to establish Lactobacillus casei in a completely automated Streptococcus mutans-based artificial mouth model and to investigate secondary caries inhibiting properties of glass ionomer cements / resin composite groups in vitro. METHODS: Sixty extracted, caries-free human third molars were used for preparation of standardized class-V-cavities. Specimens were restored with a resin-modified (Photac Fil; PF) as well as a conventional glass ionomer cement (Ketac Molar; KM) and one resin composite bonded with and without conduction of etch-and-rinse technique (Prime&Bond NT, Ceram X mono; C+ERT, C)...
March 12, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Amo Dal Piva, Lpc Contreras, F C Ribeiro, L C Anami, Sea Camargo, Aoc Jorge, M A Bottino
INTRODUCTION: This study evaluated the morphology, biofilm formation, and viability of human gingival fibroblasts in contact with two monolithic ceramics after two different finishing techniques: polishing or glazing. For this, 92 blocks (4.5 × 4.5 × 1.5 mm) of each ceramic were made using high translucency zirconia partially stabilized by yttrium (YZHT) and lithium silicate reinforced by zirconium (ZLS). METHODS AND MATERIALS: Blocks were sintered and then divided into glazing (g) or polishing (p) surface finish...
March 13, 2018: Operative Dentistry
Pei Lin Zhuang, Li Xia Yu, Juan Kun Liao, Yan Zhou, Huan Cai Lin
BACKGROUND: The VicRK two-component signalling system regulates virulence and cariogenicity in Streptococcus mutans (S. mutans). The purpose of this study was to explore the genetic polymorphisms of the vicR and vicK genes, which are associated with dental caries in children with S. mutans. METHODS: In this study, 121 (from each group) clinical S. mutans strains were isolated from caries-free children and children with high-severity caries to sequence the vicR and vicK genes...
March 12, 2018: BMC Oral Health
Thamires Priscila Cavazana, Juliano Pelim Pessan, Thayse Yumi Hosida, Douglas Roberto Monteiro, Alberto Carlos Botazzo Delbem
OBJECTIVE: This study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans. DESIGN: Biofilms were developed for 96 h, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 min. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 min after removal...
March 6, 2018: Archives of Oral Biology
Alejandro Avilés-Reyes, Irlan Almeida Freires, Jessica K Kajfasz, Dicler Barbieri, James H Miller, José A Lemos, Jacqueline Abranches
We report the whole genome sequence (WGS) of the serotype e Cbm+ strain LAR01 of Streptococcus mutans, a dental pathogen frequently associated with extra-oral infections. The LAR01 genome is a single circular chromosome of 2.1 Mb with a GC content of 36.96%. The genome contains 15 PTS gene clusters, 7 cell wall-anchored (LPxTG) proteins, all genes required for the development of natural competence and genes coding for mutacins VI and K8. Interestingly, the cbm gene is genetically linked to a putative type VII secretion system that has been found in Mycobacteria and few other gram-positive bacteria...
March 9, 2018: Molecular Oral Microbiology
Nancy J Lin, Courtney Keeler, Alison M Kraigsley, Jing Ye, Sheng Lin-Gibson
OBJECTIVE: Resin-based composites are known to elute leachables that include unincorporated starting materials. The objective of this work was to determine the effect of common dental monomers and initiators on Streptococcus mutans biofilm metabolic activity and biomass. METHODS: S. mutans biofilms were inoculated in the presence of bisphenol A glycerolate dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), camphorquinone (CQ), and ethyl 4-(dimethylamino)benzoate (4E) at 0...
March 6, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Josiane de Almeida, Andrea L Pimenta, Ulisses A Pereira, Luiz C A Barbosa, Michel A Hoogenkamp, Suzette V van der Waal, Wim Crielaard, Wilson T Felippe
This study evaluated the inhibitory effects of lactams on Streptococcus mutans, Enterococcus faecalis, and Candida glabrata multispecies biofilm formation. γ-Alkylidene-γ-lactams 1, 2, and 3 [solubilized in 3.5% dimethyl sulfoxide (DMSO)] were tested. Glass coverslips were conditioned with either the lactams or 3.5% DMSO (control) for 1 h, inoculated with microbial cultures, and incubated for 48 h. To assess the effect of the lactams on biofilm formation, the following parameters were determined: the biofilm biomass (by both crystal violet staining and protein determination); the amount of insoluble polysaccharides of the extracellular matrix; and the number of viable and total cells [by both colony-forming unit counting and quantitative real-time PCR (qPCR)]...
March 8, 2018: European Journal of Oral Sciences
Fei-Long Li, Ying Shi, Jiu-Xun Zhang, Jian Gao, Ye-Wang Zhang
A novel nicotinamide adenine dinucleotide (NADH) oxidase from Streptococcus mutans ATCC 25175 (SmNox) was cloned and overexpressed in Escherichia coli BL21 (DE3). Sequence analysis revealed an open reading frame of 1374 bp, capable of encoding a polypeptide of 457 amino acid residues. The molecular mass of the purified SmNox was estimated to be ∼49.9 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified SmNox had the highest specific activity of 281.2 U·mg-1 at optimal pH and temperature of 7...
March 4, 2018: International Journal of Biological Macromolecules
Jonatas Rafael de Oliveira, Kely Karina Belato, Felipe Eduardo de Oliveira, Antonio Olavo Cardoso Jorge, Samira Esteves Afonso Camargo, Luciane Dias de Oliveira
This study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours...
March 2018: General Dentistry
Meng-Ying Mao, Meng Li, Lei Lei, Jia-Xin Yin, Ying-Ming Yang, Tao Hu
Streptococcus mutans is an important factor in the etiology and pathogenesis of dental caries, largely owing to its ability to form a stable biofilm. Previous animal studies have indicated that rnc could decrease the amount of sulcal caries, and that the downregulation of cariogenicity might be due to its capacity to disrupt biofilm formation. However, the biofunctions by which rnc is involved in biofilm formation remain to be elucidated. In this study, we further investigate the role of rnc based on the study of mature biofilm...
March 6, 2018: Caries Research
Pratick Khara, Saswat Sourav Mohapatra, Indranil Biswas
Streptococcus mutans, the primary aetiological agent of dental caries, is one of the major bacteria of the human oral cavity. The pathogenicity of this bacterium is attributed not only to the expression of virulence factors, but also to its ability to respond and adapt rapidly to the ever-changing conditions of the oral cavity. The two-component signal transduction system (TCS) CovR/S plays a crucial role in virulence and stress response in many streptococci. Surprisingly, in S. mutans the response regulator CovR appears to be an orphan, as the cognate sensor kinase, CovS, is absent in all the strains...
March 5, 2018: Microbiology
Yufei Wang, Xiuqing Wang, Wentao Jiang, Kun Wang, Junyuan Luo, Wei Li, Xuedong Zhou, Linglin Zhang
Cariogenic virulence factors of Streptococcus mutans include acidogenicity, aciduricity, and extracellular polysaccharides (EPS) synthesis. The de novo designed antimicrobial peptide GH12 has shown bactericidal effects on S. mutans , but its interaction with virulence and regulatory systems of S. mutans remains to be elucidated. The objectives were to investigate the effects of GH12 on virulence factors of S. mutans , and further explore the function mechanisms at enzymatic and transcriptional levels. To avoid decrease in bacterial viability, we limited GH12 to subinhibitory levels...
2018: Journal of Oral Microbiology
Shuhei Naka, Kaoruko Wato, Rina Hatakeyama, Rena Okawa, Ryota Nomura, Kazuhiko Nakano
Background : We previously reported that intravenous administration of Streptococcus mutans strain TW871 caused typical non-alcoholic steatohepatitis (NASH)-like findings in a high-fat diet (HFD) mouse model at 16 weeks after initiating the experiment. Objective : The purpose of the present study was to analyse mice administered S. mutans TW871 fed a HFD for various periods of time. Methods : First, 6-week-old C57BL/6J mice were fed an HFD for 4 weeks, then TW871 (1 × 107 CFU) or phosphate-buffered saline (PBS) were intravenously administered...
2018: Journal of Oral Microbiology
Meng Zhang, Yuqiao Zheng, Yuhong Li, Han Jiang, Yuping Huang, Minquan Du
Acid resistance is one of key properties assisting the survival of cariogenic bacteria in a dental caries environment, but only a few genes conferring acid resistance have been identified to data. Functional metagenomics provides a systematic method for investigating commensal DNA to identify genes that encode target functions. Here, the host strain Escherichia coli DH10B and a constructed bidirectional transcription vector pSKII+ -lacZ contributed to the construction of a metagenomic library, and 46.6 Mb of metagenomic DNA was cloned from carious supragingival plaque of 8children along with screening for lethal functionality...
2018: Journal of Oral Microbiology
Xanthippi Chatzistavrou, Anna Lefkelidou, Lambrini Papadopoulou, Eleni Pavlidou, Konstantinos M Paraskevopoulos, J Christopher Fenno, Susan Flannagan, Carlos González-Cabezas, Nikos Kotsanos, Petros Papagerakis
Aim: Antimicrobial and bioactive restorative materials are needed to develop a bacteria free environment and tight bond with the surrounding tissue, preventing the spread of secondary caries and thus extending the lifetime of dental restorations. The characteristic properties of new dental bioactive and antibacterial composites are presented in this work. The new composites have been microstructurally characterized and both long and short term properties have been studied. Methods: The Ag-doped sol-gel derived bioactive glass (Ag-BG) was incorporated into resin composite in concentrations 5, 10, and 15 wt...
2018: Frontiers in Physiology
Zhuan-Ling Li, Xiao-Hu Xu, Xuan Chen, Xin-Yu Wu, Wang-Hong Zhao
OBJECTIVE: To evaluate the effect of SMU.2055 gene on acid resistance of Streptococcus mutans. METHODS: A SMU.2055-dificient mutant strain of S. mutans was constructed using homologous recombination technique. The growth of the wild-type and mutant strains was monitored in both normal and acidic conditions. The lethal pH level, glycolysis, proton permeability, cell permeability and biofilm formation of the two strains were compared. RESULTS: PCR and sequence analyses verified the successful construction of the SMU...
February 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Marsha A Voelker, Kimberly S Bray, Sally A Elledge, Julie Sutton, JoAnna M Scott
Purpose: To compare the effectiveness of an antigen-specific Streptococcus mutans (S. mutans) chairside test to a culture based S. mutans test. Methods: Fifty-three patients receiving dental hygiene care at the University of Missouri-Kansas City, School of Dentistry were enrolled in the study. Stimulated saliva was collected from the patients and utilized for both bacteria tests. The antigen-specific test was compared to the culture-based bacteria test and to a caries risk assessment measuring sensitivity and specificity...
February 2018: Journal of Dental Hygiene: JDH
Fabrizio Pantanella, Valerio Iebba, Francesco Mura, Luciana Dini, Valentina Totino, Bruna Neroni, Giulia Bonfiglio, Trancassini Maria, Claudio Passariello, Serena Schippa
The present study aimed to characterize the behavior of Bdellovibrio bacteriovorus in the presence of Staphylococcus aureus. B. bacteriovorus was co-cultured with S. aureus or Pseudomonas aeruginosa or Streptococcus mutans, in planktonic and sessile conditions. Co-cultures were studied by Field-Emission Scanning Electron Microscopy (FESEM), Scanning Transmission Electron Microscopy (STEM), turbidimetry, quantitative PCR (qPCR), and sequencing of gene Bd0108 of B. bacteriovorus. Results indicated that B. bacteriovorus comparably inhibited planktonic growth of P...
March 2, 2018: New Microbiologica
Lee-Yee Chong, Jan E Clarkson, Lorna Dobbyn-Ross, Smriti Bhakta
BACKGROUND: Slow-release fluoride devices have been investigated as a potentially cost-effective method of reducing dental caries in people with high risk of disease. This is the second update of the Cochrane Review first published in 2006 and previously updated in 2014. OBJECTIVES: To evaluate the effectiveness and safety of different types of slow-release fluoride devices on preventing, arresting, or reversing the progression of carious lesions on all surface types of primary (deciduous) and permanent teeth...
March 1, 2018: Cochrane Database of Systematic Reviews
Mohammad Hossein Toodehzaeim, Hengameh Zandi, Hamidreza Meshkani, Azadeh Hosseinzadeh Firouzabadi
Statement of the Problem: Orthodontic appliances facilitate microbial plaque accumulation and increase the chance of white spot lesions. There is a need for new plaque control methods independent of patient's cooperation. Purpose: The aim of this study was to determine the effects of incorporating copper oxide (CuO) nanoparticles on antimicrobial properties and bond strength of orthodontic adhesive. Materials and Method: CuO nanoparticles were added to the composite transbond XT at concentrations of 0...
March 2018: Journal of Dentistry
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