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Streptococcus mutans

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https://www.readbyqxmd.com/read/28930642/microbial-complexes-and-caries-in-17-year-olds-with-and-without-streptococcus-mutans
#1
L Eriksson, P Lif Holgerson, A Esberg, I Johansson
Streptococcus mutans is a key bacterial species in the caries process, which affects >90% of the population worldwide. However, other acidogenic and aciduric/acidophilic species may contribute to disease development. In Sweden, a country with low prevalences of caries and S. mutans, a significant portion of caries-affected adolescents lack detectable levels of S. mutans. The objectives of the present study were 1) to characterize the tooth biofilm and saliva microbiota of adolescents with caries disease, with or without detectable S...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28924033/rgpf-is-required-for-maintenance-of-stress-tolerance-and-virulence-in-streptococcus-mutans
#2
C J Kovacs, R C Faustoferri, R G Quivey
Bacterial cell wall dynamics have been implicated as important determinants of cellular physiology, stress tolerance and virulence. In Streptococcus mutans, the cell wall is composed primarily of a rhamnose-glucose polysaccharide (RGP) linked to the peptidoglycan. Despite extensive studies describing its formation and composition, the potential roles for RGP in S. mutans biology have not been well investigated. The present study characterizes the impact of RGP disruption as a result of deletion of rgpF, the gene encoding a rhamnosyltransferase involved in construction of the core poly-rhamnose backbone of RGP...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28922632/antibacterial-and-anti-biofouling-coating-on-hydroxyapatite-surface-based-on-peptide-modified-tannic-acid
#3
Xiao Yang, Pei Huang, Haihuan Wang, Shuang Cai, Yixue Liao, Ziqin Mo, Xinyuan Xu, Chunmei Ding, Changsheng Zhao, Jianshu Li
Hydroxyapatite-based implant is widely used in the fields of artificial teeth and bones. However, the problem of microbial communities on hydroxyapatite surfaces remain a great challenge. In order to inhibit bacterial adhesion and activity in a long term, a salivary acquired pellicle (SAP) bioinspired tannic acid (SAP3-TA) is synthesized and characterized. The SAP inspired peptide sequence DDDEEK has a good adsorption performance on the surface of hydroxyapatite (HA). Tannic acid (TA) is a natural polyphenolic compound that can be extracted from green tea, and it has been proved to have an effective antibacterial property...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28921941/the-lactococcus-lactis-kf147-nonribosomal-peptide-synthetase-polyketide-synthase-system-confers-resistance-to-oxidative-stress-during-growth-on-plant-leaf-tissue-lysate
#4
Benjamin L Golomb, Annabelle O Yu, Laurynne C Coates, Maria L Marco
Strains of Lactococcus lactis isolated from plant tissues possess adaptations that support their survival and growth in plant-associated microbial habitats. We previously demonstrated that genes coding for a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) system involved in production of an uncharacterized secondary metabolite are specifically induced in L. lactis KF147 during growth on plant tissues. Notably, this NRPS/PKS has only been identified in plant-isolated strains of L. lactis...
September 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28921547/a-clinical-randomized-study-on-the-influence-of-dental-whitening-on-streptococcus-mutans-population
#5
André Luiz Fraga Briso, Úrsula Aparecida Escalero Silva, Morganna Borges de Almeida Souza, Vanessa Rahal, Elerson Gaetti Jardim Júnior, Luciano Tavares Angelo Cintra
Dental whitening with peroxides has been popularized through the at-home technique, which employs low concentrations of peroxide applied on individual trays. However, there are few clinical trials reporting the effects of its continuous use on oral microbiota. Thus, the purpose of the present clinical, randomized study was to evaluate the influence of at-home whitening treatment on Streptococcus mutans in saliva, buccal mucosa, and subgingival and supragingival plaque. Thirty volunteers were randomly divided into 2 study groups (n=15) according to the whitening therapy: G CP- whitening using 10% carbamide peroxide 4 hours daily for 21 days and G HP- whitening using 6% hydrogen peroxide 1...
September 16, 2017: Australian Dental Journal
https://www.readbyqxmd.com/read/28920437/real-time-metabolic-interactions-between-two-bacterial-species-using-a-carbon-based-ph-microsensor-as-a-scanning-electrochemical-microscopy-secm-probe
#6
Vrushali Subhash Joshi, Partha Sarathi Sheet, Nyssa Cullin, Jens Kreth, Dipankar Koley
We have developed a carbon-based, fast-response potentiometric pH microsensor for use as a scanning electrochemical microscopy (SECM) chemical probe to quantitatively map the microbial metabolic exchange between two bacterial species, commensal Streptococcus gordonii and pathogenic Streptococcus mutans. The 25-μm diameter H+ ion-selective microelectrode or pH microprobe showed a Nernstian slope of 59 mV/pH and high selectivity against major ions such Na+, K+, Ca2+ and Mg2+. In addition, the unique conductive membrane composition aided us in performing an amperometric approach curve to position the probe and obtain a high-resolution pH map of the microenvironment produced by the lactate-producing S...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28916465/corrosive-effects-of-fluoride-on-titanium-under-artificial-biofilm
#7
Azusa Fukushima, Gen Mayanagi, Keiichi Sasaki, Nobuhiro Takahashi
PURPOSE: This study aimed to investigate the effect of sodium fluoride (NaF) on titanium corrosion using a biofilm model, taking environmental pH into account. METHODS: Streptococcus mutans cells were used as the artificial biofilm, and pH at the bacteria-titanium interface was monitored after the addition of 1% glucose with NaF (0, 225 or 900ppmF) at 37°C for 90min. In an immersion test, the titanium samples were immersed in the NaF solution (0, 225 or 900ppm F; pH 4...
September 12, 2017: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/28916389/removal-and-killing-of-multispecies-endodontic-biofilms-by-n-acetylcysteine
#8
Young-Suk Choi, Cheul Kim, Ji-Hoi Moon, Jin-Yong Lee
Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy...
September 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28916044/antibiotic-affinity-strategy-for-bioluminescent-detection-of-viable-gram-positive-bacteria-using-daptomycin-as-recognition-agent
#9
Mengyao Wang, Yue Wu, Yong He, Xiaoxiao Su, Hui Ouyang, Zhifeng Fu
A bioluminescent method was proposed for rapid detection of viable Gram-positive bacteria based on a novel antibiotic-affinity strategy on a magnetic beads (MBs) platform. Daptomycin, a highly efficient lipopeptide antibiotic for Gram-positive bacteria, was used as a recognition agent to functionalize MBs. The daptomycin-functionalized MBs showed high capture and concentration efficiency for Gram-positive bacteria due to the strong binding between daptomycin and bacterial cell membrane in the presence of Ca(2+) ion...
September 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28914242/assessment-of-plaque-regrowth-with-a-probiotic-toothpaste-containing-lactobacillus-paracasei-a-spectrophotometric-study
#10
Shruti Srinivasan, Bhojraj Nandlal, M Vsst Subba Rao
BACKGROUND: Probiotics are live microorganisms which when administered in adequate amounts confer health benefits on the host. Commonly, most of the organisms ascribed as having probiotic properties belong to the genera Lactobacillus and Bifidobacterium and milk is the most commonly used vehicle. OBJECTIVES: The study was aimed at analyzing the biofilm formation by plaque regrowth method upon the usage of a probiotic toothpaste containing Lactobacillus paracasei by measuring the optical density using a spectrophotometer...
October 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28914240/comparison-of-candida-species-isolated-from-children-with-and-without-early-childhood-caries-a-descriptive-cross-sectional-study
#11
M S Beena, Faizal C Peedikayil, M B GufranAfmed, T P Chandru, K Soni, N Dhanesh
BACKGROUND: Early childhood caries (ECC) is characterized by the presence of one or more decayed, missing (due to caries), or filled teeth surfaces in any primary tooth, in a child below 6 years of age. Although ECC is primarily associated with high levels of maternal Streptococcus mutans, there has been an increased interest in finding the relationship between oral fungal flora and dental caries. OBJECTIVE: The aim of the study is to identify and characterize the Candida species and to compare the candidal isolates in children with ECC and without ECC...
October 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28904323/effects-of-bittern-water-on-cariogenic-bacteria-and-saliva-secretion
#12
Mariko Ohtsu, Muneaki Tamura, Hideto Sasaki, Shuichi Sato, Masatake Asano
The effects of bittern water (BW), obtained from the ocean floor, on cariogenic bacteria and saliva secretion were examined. Streptococcus mutans was mixed with BW for 1, 3, 5, 10, and 20 min to explore the bactericidal effects of BW against cariogenic bacteria. Bacterial viability was calculated by counting the number of colony-forming units on Brain Heart Infusion agar plates. The results indicated a bacterial viability of more than 35% even after 20 min of incubation. Subsequently, the effects of BW on saliva secretion and the salivary concentration of secretory IgA (sIgA) were examined...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/28902637/development-of-fish-collagen-bioactive-glass-chitosan-composite-nanofibers-as-a-gtr-gbr-membrane-for-inducing-periodontal-tissue-regeneration
#13
Tian Zhou, Xin Liu, Baiyan Sui, Chao Liu, Xiumei Mo, Jiao Sun
The development of a guided tissue or bone regeneration (GTR/GBR) membrane with excellent performance has been a major challenge in the biomedical field. The present study was designed to prepare a biomimetic electrospun fish collagen/bioactive glass/chitosan (Col/BG/CS) composite nanofiber membrane and determine its structure, mechanical property, antibacterial activity, and biological effects on human periodontal ligament cells (HPDLCs). The effects of this composite membrane on inducing periodontal tissue regeneration were evaluated using a dog class II furcation defect model...
September 13, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28902253/bacterial-adhesion-on-conventional-and-self-ligating-metallic-brackets-after-surface-treatment-with-plasma-polymerized-hexamethyldisiloxane
#14
Rogerio Amaral Tupinambá, Cristiane Aparecida de Assis Claro, Cristiane Aparecida Pereira, Celestino José Prudente Nobrega, Ana Paula Rosifini Alves Claro
Introduction: Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion. Methods: Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment...
July 2017: Dental Press Journal of Orthodontics
https://www.readbyqxmd.com/read/28902250/effect-of-cpp-acp-paste-with-and-without-co2-laser-irradiation-on-demineralized-enamel-microhardness-and-bracket-shear-bond-strength
#15
Nasrin Farhadian, Loghman Rezaei-Soufi, Seyed Farzad Jamalian, Maryam Farhadian, Shahrzad Tamasoki, Milad Malekshoar, Bahareh Javanshir
Introduction: Many patients seeking orthodontic treatment already have incipient enamel lesions and should be placed under preventive treatments. The aim of this in vitro study was to evaluate the effect of CPP-ACP paste and CO2 laser irradiation on demineralized enamel microhardness and shear bond strength of orthodontic brackets. Methods: Eighty caries-free human premolars were subjected to a demineralization challenge using Streptococcus mutans. After demineralization, the samples were randomly divided into five equal experimental groups: Group 1 (control), the brackets were bonded without any surface treatment; Group 2, the enamel surfaces were treated with CPP-ACP paste for 4 minutes before bonding; Group 3, the teeth were irradiated with CO2 laser beams at a wavelength of 10...
July 2017: Dental Press Journal of Orthodontics
https://www.readbyqxmd.com/read/28898513/characterisation-of-a-sucrose-independent-in-vitro-biofilm-model-of-supragingival-plaque
#16
Kota Tsutsumi, Masato Maruyama, Akira Uchiyama, Ken-Ichiro Shibasaki
OBJECTIVES: Sugar consumption has been decreasing in Japan, suggesting higher rates of sucrose-independent supragingival plaque formation. For developing an in vitro biofilm model of sucrose-independent supragingival plaque, this study aimed to investigate the compositions and functions on contributing to cariogenicity in comparison with sucrose-dependent biofilm. MATERIALS AND METHODS: An in vitro multispecies biofilm containing Actinomyces naeslundii, Streptococcus gordonii, S...
September 12, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28898421/tooth-bleaching-with-low-temperature-plasma-lowers-surface-roughness-and-streptococcus-mutans-adhesion
#17
S-H Nam, S-M Ok, G-C Kim
AIM: To evaluate the structural-morphological changes in enamel surface roughness and Streptococcus mutans adhesion after tooth bleaching using plasma in combination with a low concentration of 15% carbamide peroxide (CP). METHODOLOGYS: Sixty pairs of premolars were randomly assigned to the treatment groups (n = 30; buccal surface, Groups 1A/2A) or controls (n = 30; palatal surface, Groups 1B/2B). Group 1A received a low concentration of 15% CP and low-temperature plasma...
September 12, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28894441/the-fitness-cost-of-fluoride-resistance-for-different-streptococcus-mutans-strains-in-biofilms
#18
Yanling Cai, Ying Liao, Bernd W Brandt, Xi Wei, Hongyan Liu, Wim Crielaard, Cor Van Loveren, Dong Mei Deng
The cariogenic bacterium Streptococcus mutans can develop stable resistance to fluoride through chromosomal mutations in vitro. Fluoride-resistant S. mutans has seldom been isolated in clinical settings, despite the wide application of fluoride in oral-care products. One explanation is that the fluoride-resistant S. mutans strains have decreased fitness. However, so far, there has been no conclusive evidence to support this idea. The aim of this study was to investigate the fitness cost of 48-h biofilms of two fluoride-resistant S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28893032/antibacterial-activity-of-freshly-prepared-ozonated-water-and-chlorhexidine-on-mutans-streptococcus-when-used-as-an-oral-rinse-a-randomised-clinical-study
#19
Lavanya Anumula, Kv Suneel Kumar, Chnv Murali Krishna, K Sree Lakshmi
INTRODUCTION: Dental caries is one of the most common causes of morbidity of the tooth. Attempts have been made to reduce the pathogen population size i.e., Mutans Streptococci (MS) to demote the incidence of caries and increase the resistance of the tooth to cariogenic attack. AIM: To evaluate the antibacterial efficacy of freshly prepared ozonated water, in proposing it as an alternative mouth rinse on MS in comparison to Chlorhexidine (CHX). MATERIALS AND METHODS: Subjects with high caries incidence and MS counts more than 105 Colony Forming Unit (CFU) were selected and divided by block randomization into two groups of 23 subjects each...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892899/first-detection-and-characterization-of-streptococcus-dentapri-from-caries-active-subject
#20
Hamzah Abdulrahman Salman, R Senthil Kumar, N Chaitanya Babu, Khalid Imran
INTRODUCTION: Mutans streptococci (MS) are a group of oral bacteria generally regarded as the principal agents in the pathogenesis of dental caries. AIM: The study aimed was characterize S. dentapri based on phylogenetic analysis and phenotypic methods from Caries Active Subject. MATERIALS AND METHODS: While sequencing MS species which were isolated from 65 caries active subjects, one strain of S. dentapri was detected. Dental plaque samples were processed and cultured on mitis salivarius bacitracin agar...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
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