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https://www.readbyqxmd.com/read/29643450/author-correction-methods-for-scarless-selection-free-generation-of-human-cells-and-allele-specific-functional-analysis-of-disease-associated-snps-and-variants-of-uncertain-significance
#1
Nicole B Coggins, Jacob Stultz, Henriette O'Geen, Luis G Carvajal-Carmona, David J Segal
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29616178/scarless-breast-reconstruction-indications-and-techniques-for-optimizing-aesthetic-outcomes-in-autologous-breast-reconstruction
#2
Wojciech Dec
Breast reconstruction that leaves no visible scars on the breast is possible for a subset of patients. This article reviews a cohort of 10 patients who underwent 14 autologous breast reconstructions. To achieve a reconstruction without visible breast scars, the mastectomy and autologous reconstruction are carried out through a periareolar incision. At the completion of the reconstruction, a small skin paddle is externalized through the mastectomy incision and in a subsequent stage entirely incorporated into a nipple areola reconstruction...
February 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29609045/crispr-cas9-editing-genome-of-extremophile-halomonas-spp
#3
Qin Qin, Chen Ling, Yiqing Zhao, Tian Yang, Jin Yin, Yingying Guo, Guo Qiang Chen
Extremophiles are suitable chassis for developing the next generation industrial biotechnology (NGIB) due to their resistance to microbial contamination. However, engineering extremophiles are not an easy task. Halomonas, an industrially interesting halophile able to grow under unsterile and continuous conditions in large-scale processes, can only be engineered using suicide plasmid-mediated two-step homologous recombination which is very laborious and time-consuming (up to half a year). A convenient approach for the engineering of halophiles that can possibly be extended to other extremophiles is therefore urgently required...
March 30, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29596891/connexin-43-regulates-the-expression-of-wound-healing-related-genes-in-human-gingival-and-skin-fibroblasts
#4
Rana Tarzemany, Guoqiao Jiang, Jean X Jiang, Corrie Gallant-Behm, Colin Wiebe, David A Hart, Hannu Larjava, Lari Häkkinen
Fibroblasts are the most abundant connective tissue cells and play an important role in wound healing. It is possible that faster and scarless wound healing in oral mucosal gingiva relative to skin may relate to the distinct phenotype of the fibroblasts residing in these tissues. Connexin 43 (Cx43) is the most ubiquitous Cx in skin (SFBLs) and gingival fibroblasts (GFBLs), and Cx43 assembles into hemichannels (HCs) and gap junctions (GJs) on the cell membrane. We hypothesized that SFBLs and GFBLs display distinct expression or function of Cx43, and that this may partly underlie the different wound healing outcomes in skin and gingiva...
March 26, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29595723/inner-scar-umbilicus-new-horizons-for-vertical-abdominoplasty
#5
Flavio H Mendes, Fausto Viterbo, Ana L A P Luna
BACKGROUND: It is reasonable that deflated tissue in massive weight loss patients may demand not only lifting but also some extent of central body tightening, by the fleur-de-lis procedure. Although achieving nice contouring results, poor visible scars, including umbilical complications, have restricted the indications for the anterior vertical approach. The purpose of this article is to present the authors' experience with the inner scar umbilical reconstruction, reflecting overall results in vertical abdominoplasties...
April 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29593327/two-succeeding-fibroblastic-lineages-drive-dermal-development-and-the-transition-from-regeneration-to-scarring
#6
Dongsheng Jiang, Donovan Correa-Gallegos, Simon Christ, Ania Stefanska, Juan Liu, Pushkar Ramesh, Vijayanand Rajendran, Martina M De Santis, Darcy E Wagner, Yuval Rinkevich
During fetal development, mammalian back-skin undergoes a natural transition in response to injury, from scarless regeneration to skin scarring. Here, we characterize dermal morphogenesis and follow two distinct embryonic fibroblast lineages, based on their history of expression of the engrailed 1 gene. We use single-cell fate-mapping, live three dimensional confocal imaging and in silico analysis coupled with immunolabelling to reveal unanticipated structural and regional complexity and dynamics within the dermis...
April 2018: Nature Cell Biology
https://www.readbyqxmd.com/read/29552744/re-laparoscopic-2port-varicocelectomy-with-scarless-periumblical-mini-incision-initial-experience-in-approach-and-outcomes
#7
Amir H Kashi
No abstract text is available yet for this article.
March 18, 2018: Urology Journal
https://www.readbyqxmd.com/read/29529861/3d-protein-based-bilayer-artificial-skin-for-guided-scarless-healing-of-3rd-degree-burn-wounds-in-vivo
#8
Mazaher Gholipourmalekabadi, Alexander M Seifalian, Aleksandra M Urbanska, Mir Davood Omrani, John G Hardy, Zahra Madjd, Seyed Mahmoud Hashemi, Hossein Ghanbarian, Peiman Brouki Milan, Masoud Mozafari, Rui L Reis, Subhas C Kundu, Ali Samadikuchaksaraei
Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds...
March 12, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29521753/facial-defect-reconstruction-using-the-true-scarless-pre-expanded-forehead-flap
#9
Shan Zhu, Yuanbo Liu, Mengqing Zang, Bo Chen, Shanshan Li, Bingjian Xue, Tinglu Han
OBJECTIVE: This clinical study describes a reconstructive method for facial soft-tissue defects that uses the pre-expanded forehead flap and minimizes donor site morbidities. METHODS: The surgery was subdivided into 3 stages. First stage, an appropriately sized expander was buried underneath the forehead. Second stage, after adequate inflation of the expander, a forehead flap based on the frontal branches of the superficial temporal artery was raised, and the distal portion of the flap was used to reconstruct the facial defect...
March 8, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29507284/microhomology-assisted-scarless-genome-editing-in-human-ipscs
#10
Shin-Il Kim, Tomoko Matsumoto, Harunobu Kagawa, Michiko Nakamura, Ryoko Hirohata, Ayano Ueno, Maki Ohishi, Tetsushi Sakuma, Tomoyoshi Soga, Takashi Yamamoto, Knut Woltjen
Gene-edited induced pluripotent stem cells (iPSCs) provide relevant isogenic human disease models in patient-specific or healthy genetic backgrounds. Towards this end, gene targeting using antibiotic selection along with engineered point mutations remains a reliable method to enrich edited cells. Nevertheless, integrated selection markers obstruct scarless transgene-free gene editing. Here, we present a method for scarless selection marker excision using engineered microhomology-mediated end joining (MMEJ)...
March 5, 2018: Nature Communications
https://www.readbyqxmd.com/read/29493878/developing-a-piggybac-transposon-system-and-compatible-selection-markers-for-insertional-mutagenesis-and-genome-engineering-in-yarrowia-lipolytica
#11
James M Wagner, Eden V Williams, Hal S Alper
Yarrowia lipolytica is a non-conventional yeast of interest to the biotechnology industry. However, the physiology, metabolism, and genetic regulation of Y. lipolytica diverge significantly from more well-studied and characterized yeasts such as Saccharomyces cerevisiae. To develop additional genetic tools for this industrially relevant host, we adapt the piggyBac transposon system to enable efficient generation of genome-wide insertional mutagenesis libraries and introduction of scarless, footprint-free genomic modifications in Y...
March 1, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29420124/embryonic-skin-development-and-repair
#12
Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker
Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds. This article reviews the embryonic development of the skin and outlines the structural and functional differences in adult and fetal wound healing phenotypes...
February 8, 2018: Organogenesis
https://www.readbyqxmd.com/read/29417340/trans-umbilical-two-port-laparoscopic-suturing-of-the-inguinal-hernia-defect-with-percutaneous-assistance-a-safe-and-efficient-scarless-surgery-for-pediatric-inguinal-hernia-repair
#13
T Shou, H Zhong, F Wang
PURPOSE: This study was aimed to describe the technique of the modified trans-umbilical two-port laparoscopic suturing (M-TTLS), and show its feasibility and efficacy to treat pediatric inguinal hernia (PIH). METHODS: After general anesthesia, the patient was placed in the Trendelenburg position. Two trocars were set on umbilicus and a pneumoperitoneum was established at 8-12 mmHg. Under direct vision, a long syringe needle was punctured into the preperitoneal space above the internal inguinal ring (IIR)...
February 7, 2018: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/29392093/mechanical-forces-in-cutaneous-wound-healing-emerging-therapies-to-minimize-scar-formation
#14
REVIEW
Leandra A Barnes, Clement D Marshall, Tripp Leavitt, Michael S Hu, Alessandra L Moore, Jennifer G Gonzalez, Michael T Longaker, Geoffrey C Gurtner
Significance: Excessive scarring is major clinical and financial burden in the United States. Improved therapies are necessary to reduce scarring, especially in patients affected by hypertrophic and keloid scars. Recent Advances: Advances in our understanding of mechanical forces in the wound environment enable us to target mechanical forces to minimize scar formation. Fetal wounds experience much lower resting stress when compared with adult wounds, and they heal without scars. Therapies that modulate mechanical forces in the wound environment are able to reduce scar size...
February 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29375275/gibson-deletion-a-novel-application-of-isothermal-in-vitro-recombination
#15
Swara Kalva, Jef D Boeke, Paolo Mita
Background: Recombinant DNA technology is today a fundamental tool for virtually all biological research fields. Among the many techniques available for the construction of a "custom DNA" molecule, the isothermal in vitro assembly, or Gibson assembly, allows for an efficient, one-step, scarless recombination-based assembly. Results: Here, we apply and characterize the use of Gibson assembly for the deletion of DNA sequences around a DNA cut. This method, that we named "Gibson Deletion", can be used to easily substitute or delete one or more restriction sites within a DNA molecule...
2018: Biological Procedures Online
https://www.readbyqxmd.com/read/29369151/autologous-fat-graft-for-the-treatment-of-sighted-posttraumatic-enophthalmos-and-sunken-upper-eyelid
#16
Hui Chen, Qian Zhang, Qiu Qiu, Zhenglin Yang
PURPOSE: The treatment of enophthalmos and sunken upper eyelid is challenging. Although autologous fat graft has been widely used in breast augmentation, buttock contouring, and facial rejuvenation, its application in enophthalmos and sunken upper eyelid is not yet widely utilized. The clinical safety and value of autologous fat graft in sighted patients with enophthalmos and sunken upper eyelid are unclear. This study retrospectively analyzed the cosmetic results and safety of autologous fat graft in the correction of sighted traumatic enophthalmos and sunken upper eyelid...
January 23, 2018: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29353464/laparoscopic-2port-varicocelectomy-with-scarless-periumblical-mini-incision-initial-experience-in-approach-and-outcomes
#17
Won Ik Seo, Jong Kyou Kwon, Pil Moon Kang, Wansuk Kim, Jang Ho Yoon, Taek Sang Kim, Jae Seung Chung, Cheol Kyu Oh
PURPOSE: As with other areas, there have been many efforts for minimally invasive surgery in varicocelectomy. We present our initial experience with laparoscopic varicocelectomy with a two-port scarless periumbilical mini-incision. MATERIALS AND METHODS: The study enrolled 18 patients who underwent laparoscopic varicocelectomy with a twoportscarless periumbilical mini-incision from February 2012 to April 2013. The laparoscopic varicocelectomy was performed using two 5-mm ports at periumbilical sites in skin creases...
March 18, 2018: Urology Journal
https://www.readbyqxmd.com/read/29334788/latissimus-dorsi-flap-in-breast-reconstruction-recent-innovations-in-the-workhorse-flap
#18
Rachita Sood, Jeena M Easow, Geoffrey Konopka, Zubin J Panthaki
BACKGROUND: Surgeons employ the latissimus dorsi flap (LDF) for reconstruction of a large variety of breast cancer surgery defects, including quadrantectomy, lumpectomy, modified radical mastectomy, and others. The LDF may be used in delayed or immediate reconstruction, in combination with tissue expanders for a staged reconstruction, with implant-based immediate reconstruction, or alone as an autogenous flap. METHODS: The authors discuss the historical uses and more recent developments in the LDF...
January 2018: Cancer Control: Journal of the Moffitt Cancer Center
https://www.readbyqxmd.com/read/29316315/scarless-wound-healing-transitioning-from-fetal-research-to-regenerative-healing
#19
REVIEW
Alessandra L Moore, Clement D Marshall, Leandra A Barnes, Matthew P Murphy, Ryan C Ransom, Michael T Longaker
Since the discovery of scarless fetal skin wound healing, research in the field has expanded significantly with the hopes of advancing the finding to adult human patients. There are several differences between fetal and adult skin that have been exploited to facilitate scarless healing in adults including growth factors, cytokines, and extracellular matrix substitutes. However, no one therapy, pathway, or cell subtype is sufficient to support scarless wound healing in adult skin. More recently, products that contain or mimic fetal and adult uninjured dermis were introduced to the wound healing market with promising clinical outcomes...
March 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29310861/never-too-old-to-regenerate-wound-induced-hair-follicle-neogenesis-after-secondary-intention-healing-in-a-geriatric-patient
#20
Tak-Wah Wong, Michael Hughes, Szu-Han Wang
Wound healing is a natural process to restore the structure and function of injured or diseased tissues. Repair of a skin wound usually leads to a scar while regeneration implies fully recovery of function and structure of the damaged tissue. Adult skin wound usually heals with scar while fetal skin heals scarless. Hair regeneration in elderly scalp wound has never been observed. We reported an 80-year-old patient with a large wound on the scalp after excision of a basal cell carcinoma healed by secondary intention wound healing...
January 4, 2018: Journal of Tissue Viability
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