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https://www.readbyqxmd.com/read/29420124/embryonic-skin-development-and-repair
#1
Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker
Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds. This article reviews the embryonic development of the skin and outlines the structural and functional differences in adult and fetal wound healing phenotypes...
February 8, 2018: Organogenesis
https://www.readbyqxmd.com/read/29417340/trans-umbilical-two-port-laparoscopic-suturing-of-the-inguinal-hernia-defect-with-percutaneous-assistance-a-safe-and-efficient-scarless-surgery-for-pediatric-inguinal-hernia-repair
#2
T Shou, H Zhong, F Wang
PURPOSE: This study was aimed to describe the technique of the modified trans-umbilical two-port laparoscopic suturing (M-TTLS), and show its feasibility and efficacy to treat pediatric inguinal hernia (PIH). METHODS: After general anesthesia, the patient was placed in the Trendelenburg position. Two trocars were set on umbilicus and a pneumoperitoneum was established at 8-12 mmHg. Under direct vision, a long syringe needle was punctured into the preperitoneal space above the internal inguinal ring (IIR)...
February 7, 2018: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/29392093/mechanical-forces-in-cutaneous-wound-healing-emerging-therapies-to-minimize-scar-formation
#3
REVIEW
Leandra A Barnes, Clement D Marshall, Tripp Leavitt, Michael S Hu, Alessandra L Moore, Jennifer G Gonzalez, Michael T Longaker, Geoffrey C Gurtner
Significance: Excessive scarring is major clinical and financial burden in the United States. Improved therapies are necessary to reduce scarring, especially in patients affected by hypertrophic and keloid scars. Recent Advances: Advances in our understanding of mechanical forces in the wound environment enable us to target mechanical forces to minimize scar formation. Fetal wounds experience much lower resting stress when compared with adult wounds, and they heal without scars. Therapies that modulate mechanical forces in the wound environment are able to reduce scar size...
February 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29375275/gibson-deletion-a-novel-application-of-isothermal-in-vitro-recombination
#4
Swara Kalva, Jef D Boeke, Paolo Mita
Background: Recombinant DNA technology is today a fundamental tool for virtually all biological research fields. Among the many techniques available for the construction of a "custom DNA" molecule, the isothermal in vitro assembly, or Gibson assembly, allows for an efficient, one-step, scarless recombination-based assembly. Results: Here, we apply and characterize the use of Gibson assembly for the deletion of DNA sequences around a DNA cut. This method, that we named "Gibson Deletion", can be used to easily substitute or delete one or more restriction sites within a DNA molecule...
2018: Biological Procedures Online
https://www.readbyqxmd.com/read/29369151/autologous-fat-graft-for-the-treatment-of-sighted-posttraumatic-enophthalmos-and-sunken-upper-eyelid
#5
Hui Chen, Qian Zhang, Qiu Qiu, Zhenglin Yang
PURPOSE: The treatment of enophthalmos and sunken upper eyelid is challenging. Although autologous fat graft has been widely used in breast augmentation, buttock contouring, and facial rejuvenation, its application in enophthalmos and sunken upper eyelid is not yet widely utilized. The clinical safety and value of autologous fat graft in sighted patients with enophthalmos and sunken upper eyelid are unclear. This study retrospectively analyzed the cosmetic results and safety of autologous fat graft in the correction of sighted traumatic enophthalmos and sunken upper eyelid...
January 23, 2018: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29353464/laparoscopic-2port-varicocelectomy-with-scarless-periumblical-mini-incision-initial-experience-in-approach-and-outcomes
#6
Won Ik Seo, Jong Kyou Kwon, Pil Moon Kang, Wansuk Kim, Jang Ho Yoon, Taek Sang Kim, Jae Seung Chung, Cheol Kyu Oh
PURPOSE: As with other areas, there have been many efforts for minimally invasive surgery in varicocelectomy. We present our initial experience with laparoscopic varicocelectomy with a two-port scarless periumbilical mini-incision. MATERIALS AND METHODS: The study enrolled 18 patients who underwent laparoscopic varicocelectomy with a twoportscarless periumbilical mini-incision from February 2012 to April 2013. The laparoscopic varicocelectomy was performed using two 5-mm ports at periumbilical sites in skin creases...
January 21, 2018: Urology Journal
https://www.readbyqxmd.com/read/29334788/latissimus-dorsi-flap-in-breast-reconstruction-recent-innovations-in-the-workhorse-flap
#7
Rachita Sood, Jeena M Easow, Geoffrey Konopka, Zubin J Panthaki
BACKGROUND: Surgeons employ the latissimus dorsi flap (LDF) for reconstruction of a large variety of breast cancer surgery defects, including quadrantectomy, lumpectomy, modified radical mastectomy, and others. The LDF may be used in delayed or immediate reconstruction, in combination with tissue expanders for a staged reconstruction, with implant-based immediate reconstruction, or alone as an autogenous flap. METHODS: The authors discuss the historical uses and more recent developments in the LDF...
January 2018: Cancer Control: Journal of the Moffitt Cancer Center
https://www.readbyqxmd.com/read/29316315/scarless-wound-healing-transitioning-from-fetal-research-to-regenerative-healing
#8
REVIEW
Alessandra L Moore, Clement D Marshall, Leandra A Barnes, Matthew P Murphy, Ryan C Ransom, Michael T Longaker
Since the discovery of scarless fetal skin wound healing, research in the field has expanded significantly with the hopes of advancing the finding to adult human patients. There are several differences between fetal and adult skin that have been exploited to facilitate scarless healing in adults including growth factors, cytokines, and extracellular matrix substitutes. However, no one therapy, pathway, or cell subtype is sufficient to support scarless wound healing in adult skin. More recently, products that contain or mimic fetal and adult uninjured dermis were introduced to the wound healing market with promising clinical outcomes...
January 9, 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29310861/never-too-old-to-regenerate-wound-induced-hair-follicle-neogenesis-after-secondary-intention-healing-in-a-geriatric-patient
#9
Tak-Wah Wong, Michael Hughes, Szu-Han Wang
Wound healing is a natural process to restore the structure and function of injured or diseased tissues. Repair of a skin wound usually leads to a scar while regeneration implies fully recovery of function and structure of the damaged tissue. Adult skin wound usually heals with scar while fetal skin heals scarless. Hair regeneration in elderly scalp wound has never been observed. We reported an 80-year-old patient with a large wound on the scalp after excision of a basal cell carcinoma healed by secondary intention wound healing...
January 4, 2018: Journal of Tissue Viability
https://www.readbyqxmd.com/read/29299733/genetic-tools-for-reliable-gene-expression-and-recombineering-in-pseudomonas-putida
#10
Taylor B Cook, Jacqueline M Rand, Wasti Nurani, Dylan K Courtney, Sophia A Liu, Brian F Pfleger
Pseudomonas putida is a promising bacterial host for producing natural products, such as polyketides and nonribosomal peptides. In these types of projects, researchers need a genetic toolbox consisting of plasmids, characterized promoters, and techniques for rapidly editing the genome. Past reports described constitutive promoter libraries, a suite of broad host range plasmids that replicate in P. putida, and genome-editing methods. To augment those tools, we have characterized a set of inducible promoters and discovered that IPTG-inducible promoter systems have poor dynamic range due to overexpression of the LacI repressor...
January 3, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29224829/plasticity-of-oral-mucosal-cell-sheets-for-accelerated-and-scarless-skin-wound-healing
#11
Jong-Lyel Roh, Jaewang Lee, Eun Hye Kim, Daiha Shin
OBJECTIVES: Wound healing is generally faster and associated with less scarring in the oral mucosa than in the skin. Although rarely studied, oral mucosa equivalents may contribute to rapid, scarless cutaneous wound healing. Therefore, we examined the potential utility of our newly developed oral mucosal cell sheet in skin wound healing. MATERIALS AND METHODS: Oral mucosa and skin samples were obtained from surgical patients and Sprague-Dawley rats. Keratinocytes and fibroblasts were primarily cultured for in vitro cell expansion...
December 2017: Oral Oncology
https://www.readbyqxmd.com/read/29137618/afeap-cloning-a-precise-and-efficient-method-for-large-dna-sequence-assembly
#12
Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong, Yibin Lin
BACKGROUND: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allows scarless, modular, and reliable construction of biological pathways and circuits from basic genetic parts. METHODS: The AFEAP method requires two-round of PCRs followed by ligation of the sticky ends of DNA fragments...
November 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29118424/methods-for-scarless-selection-free-generation-of-human-cells-and-allele-specific-functional-analysis-of-disease-associated-snps-and-variants-of-uncertain-significance
#13
Nicole B Coggins, Jacob Stultz, Henriette O'Geen, Luis G Carvajal-Carmona, David J Segal
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public health. Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pipeline utilizing KASP (Kompetitive Allele-Specific PCR) genotyping technology to create scarless isogenic cell models of cancer variants in ~1 month. We successfully modeled two novel variants previously identified by our lab in the PALB2 gene in HEK239 cells, resulting in isogenic cells representing all three genotypes for both variants...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29078599/from-full-thoracotomy-to-uniportal-video-assisted-thoracic-surgery-lessons-learned
#14
REVIEW
Eliseo Passera, Gaetano Rocco
Over the last two decades, conventional video-assisted thoracic surgery (VATS) has established itself as the preferred approach for almost all thoracic surgical procedures. The procedure provides a safe and easy approach with undisputed patient benefit at a cost acceptable to the healthcare system all over the world, in large hospitals as well as underprivileged rural areas. VATS has effectively addressed the patients' right to less scarring, trauma (both of access and intrathoracic manipulation), medication, pain, hospitalization, and early return home and work...
2017: Journal of Visualized Surgery
https://www.readbyqxmd.com/read/29074845/connexin-43-hemichannels-regulate-the-expression-of-wound-healing-associated-genes-in-human-gingival-fibroblasts
#15
Rana Tarzemany, Guoqiao Jiang, Jean X Jiang, Hannu Larjava, Lari Häkkinen
Connexin 43 (Cx43) is the most ubiquitous connexin in various cells, and presents as hemichannels (HCs) and gap junctions (GJs) on the cell membrane. We have recently shown that Cx43 abundance was strongly reduced in fibroblasts of human gingival wounds, and blocking Cx43 function in cultured human gingival fibroblasts (GFBLs) strongly regulated the expression of wound healing-related genes. However, it is not known whether these responses involved Cx43 HCs or GJs. Here we show that Cx43 assembled into distinct GJ and HC plaques in GFBLs both in vivo and in vitro...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29069877/is-robot-assisted-surgery-really-scarless-surgery-immediate-reconstruction-with-a-jejunal-free-flap-for-esophageal-rupture-after-robot-assisted-thyroidectomy
#16
Seong Hoon Park, Joo Hyun Kim, Jun Won Lee, Hii Sun Jeong, Dong Jin Lee, Byung Chun Kim, In Suck Suh
Esophageal perforation is a rare but potentially fatal complication of robot-assisted thyroidectomy (RAT). Herein, we report the long-term outcome of an esophageal reconstruction with a jejunal free flap for esophageal rupture after RAT. A 33-year-old woman developed subcutaneous emphysema and hoarseness on postoperative day1 following RAT. Esophageal rupture was diagnosed by computed tomography and endoscopy, and immediate surgical exploration confirmed esophageal rupture, as well as recurrent laryngeal nerve injury...
November 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/29045533/liposuction-augmentation-mammaplasty
#17
Ryan Harris, Peter Raphael, Scott W Harris
Background: The female breast loses superior fullness and becomes more ptotic over time. Women often present to their plastic surgeon requesting reversal of this evolution. While liposuction alone has been proven to lift the breast, no solution combining augmentation and liposuction has been reported. Objectives: Herein, we introduce a technique called liposuction-augmentation mammaplasty (LAM) that can achieve "scarless" lifting or simply volume equalization prior to inserting same-sized implants...
October 16, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29042658/exosomes-secreted-by-human-adipose-mesenchymal-stem-cells-promote-scarless-cutaneous-repair-by-regulating-extracellular-matrix-remodelling
#18
Lu Wang, Li Hu, Xin Zhou, Zehuan Xiong, Chenguang Zhang, Hassan M A Shehada, Bo Hu, Jinlin Song, Lili Chen
Scar formation is an intractable medical problem that appears after skin wounds have healed. Recent research has shown that exosomes secreted by human adipose mesenchymal stem cells (ASC-Exos) can benefit wound healing. To further explore the therapeutic potential of ASC-Exos, we investigated their effects on mitigating scar formation, and the underlying mechanisms of these effects. We found that intravenous injection of ASC-Exos decreased the size of scars and increased the ratio of collagen III to collagen I in murine incisional wounds...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038745/the-contractile-phenotype-of-dermal-fetal-fibroblasts-in-scarless-wound-healing
#19
Aron Parekh, Patricia A Hebda
PURPOSE OF REVIEW: Injured skin in the mammalian fetus can heal regeneratively due to the ability of fetal fibroblasts to effectively reorganize the extracellular matrix (ECM). This process occurs without fetal fibroblasts differentiating into highly contractile myofibroblasts which cause scarring and fibrosis in adult wounds. Here, we provide a brief review of fetal wound healing and the evidence supporting a unique contractile phenotype in fetal fibroblasts. Furthermore, we discuss the biomechanical role of the ECM in driving myofibroblast differentiation in wound healing and the implications for new clinical modalities based on the biophysical properties of fetal fibroblasts...
September 2017: Current Pathobiology Reports
https://www.readbyqxmd.com/read/29036023/percutaneous-mesh-expansion-a-regenerative-wound-closure-alternative
#20
Kimberly S Khouri, Eufemiano Cardoso, Roger K Khouri
BACKGROUND: Puncture wounds in the 1-mm range usually heal without scars. Stacking rows of these punctures offers a scarless method to generate tissue by mesh expansion. The authors developed a percutaneous mesh expansion procedure and present their experience for its wound closure application. METHODS: Over a 6-year period, the authors applied percutaneous mesh expansion to 65 consecutive patients aged 58 to 101 years (mean, 72 years) with 67 full-thickness calvarial defects ranging in size from 2...
February 2018: Plastic and Reconstructive Surgery
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