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https://www.readbyqxmd.com/read/27932775/-experience-in-transumbilical-endoscopic-surgery-diagnosis-for-3-cases-of-pseudomyxoma-peritonei
#1
Li Tian, Anliu Tang, Fen Liu, Shourong Shen, Xiaoyan Wang
Three patients of pseudomyxoma peritonei who were diagnozed by transumbilical endoscopic surgery (TUES) were reviewed retrospectively from September 2014 to November 2014. Three cases of ascites patients underwent TUES were diagnozed as pseudomyxoma peritonei. All operations were successful. No open surgery or laparoscopic surgery was required. The mean operative time was (45±16) min; the mean intraoperative blood loss was 510 mL; the mean hospital stay time was 3 days. During the follow up of 911 months, no obvious scar was observed...
November 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27929521/efficient-footprint-free-human-ipsc-genome-editing-by-consolidation-of-cas9-crispr-and-piggybac-technologies
#2
Gang Wang, Luhan Yang, Dennis Grishin, Xavier Rios, Lillian Y Ye, Yong Hu, Kai Li, Donghui Zhang, George M Church, William T Pu
Genome editing of human induced pluripotent stem cells (hiPSCs) offers unprecedented opportunities for in vitro disease modeling and personalized cell replacement therapy. The introduction of Cas9-directed genome editing has expanded adoption of this approach. However, marker-free genome editing using standard protocols remains inefficient, yielding desired targeted alleles at a rate of ∼1-5%. We developed a protocol based on a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon to enable robust and highly efficient Cas9-directed genome editing, so that a parental line can be expeditiously engineered to harbor many separate mutations...
January 2017: Nature Protocols
https://www.readbyqxmd.com/read/27919731/nerve-sparing-laparoscopic-colposacropexy-using-a-percutaneous-surgical-system-a-case-report
#3
Federico Romano, Francesco Legge, Giovanni Scambia, Maurizio Guido
STUDY OBJECTIVE: To evaluate the feasibility, efficacy and safeness of nerve-sparing laparoscopic colposacropexy performed with a minimally invasive approach by using 2,9 mm Percuvance(TM) Percutaneous Surgical System (PSS) (The Percuvance(TM) System, Teleflex Inc., USA). DESIGN: Step-by-step video demonstration of the surgical technique. The Internal Department Committee approved this study. The patient was adequately informed about the possible risks and benefits of this experimental technique and a written consent agreeing to undergo the described procedure was signed...
December 2, 2016: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/27917649/percutaneous-externally-assembled-laparoscopic-peal-versus-laparoendoscopic-single-site-less-nephrectomy-in-a-porcine-model-a-prospective-randomized-blinded-study
#4
Mohamed Keheila, Jim Ken-Chie Shen, Daniel Faaborg, Patrick Yang, Salim Cheriyan, Samuel R Abourbih, Nazih Khater, Michael Hill, D Duane Baldwin
OBJECTIVE: To compare the outcomes between Laparoendoscopic single-site (LESS) and a novel Percutaneous Externally Assembled Laparoscopic (PEAL) nephrectomy in an in vivo porcine model. MATERIALS AND METHODS: Ten female farm pigs were randomized to LESS nephrectomy (5) or PEAL nephrectomy (5). Operative times, estimated blood loss, intraoperative and postoperative complications were compared. The surgeons used a Likert scale to grade difficulty of peritoneal access, port placement, tool assembly, hilar dissection, closure, and overall difficulty of surgery...
December 4, 2016: Journal of Endourology
https://www.readbyqxmd.com/read/27867546/transoral-endoscopic-mediastinal-surgery-toems-results-of-a-first-clinical-study-for-scarless-mediastinal-lymph-node-biopsies
#5
Wolfram Klemm, Steffen Frese, Gunda Leschber, André Nemat, Thomas Wilhelm
BACKGROUND: Video-assisted mediastinoscopy (VAM) represents the standard procedure for mediastinal lymph node biopsies. This operation results in a scar at prominent position at the anterior neck. Since there is a trend to less invasive procedures, natural orifice transluminal endoscopic surgery (NOTES) was introduced to different fields of surgery. Based on NOTES we developed a new approach for mediastinoscopy: transoral endoscopic mediastinal surgery (TOEMS). In previous studies using human cadavers and living pigs the feasibility of TOEMS was shown...
October 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27827431/evidence-for-a-dynamic-role-for-mononuclear-phagocytes-during-endometrial-repair-and-remodelling
#6
Fiona L Cousins, Phoebe M Kirkwood, Philippa T K Saunders, Douglas A Gibson
In women, endometrial breakdown, which is experienced as menstruation, is characterised by high concentrations of inflammatory mediators and immune cells which account for ~40% of the stromal compartment during tissue shedding. These inflammatory cells are known to play a pivotal role in tissue breakdown but their contribution to the rapid scarless repair of endometrium remains poorly understood. In the current study we used a mouse model of menstruation to investigate dynamic changes in mononuclear phagocytes during endometrial repair and remodelling...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27777621/genome-editing-of-clostridium-autoethanogenum-using-crispr-cas9
#7
Shilpa Nagaraju, Naomi Kathleen Davies, David Jeffrey Fraser Walker, Michael Köpke, Séan Dennis Simpson
BACKGROUND: Impactful greenhouse gas emissions abatement can now be achieved through gas fermentation using acetogenic microbes for the production of low-carbon fuels and chemicals. However, compared to traditional hosts like Escherichia coli or yeast, only basic genetic tools exist for gas-fermenting acetogens. To advance the process, a robust genetic engineering platform for acetogens is essential. RESULTS: In this study, we report scarless genome editing of an industrially used model acetogen, Clostridium autoethanogenum, using the CRISPR/Cas9 system...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27768643/transcanalicular-laser-assisted-dacryocystorhinostomy-with-endonasal-augmentation-in-primary-nasolacrimal-duct-obstruction-our-experience
#8
Ruchi Goel, Smriti Nagpal, Sushil Kumar, Ravi Meher, Saurabh Kamal, Sonam Garg
PURPOSE: To evaluate and compare the success rate of transcanalicular laser-assisted dacryocystorhinostomy with endonasal augmentation, with and without intubation, in patients suffering from primary acquired nasolacrimal duct obstruction, at 1 year of follow up. METHODS: A prospective, randomized interventional pilot study was conducted at a tertiary care center, in accordance with the guidelines of Declaration of Helsinki. Sixty eyes of 60 adult patients with primary acquired nasolacrimal duct obstruction were included...
October 20, 2016: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27765807/recombineering-and-i-scei-mediated-pseudomonas-putida-kt2440-scarless-gene-deletion
#9
Zhongqiu Chen, Wen Ling, Guangdong Shang
Pseudomonas putida KT2440 is a saprophytic, generally recognized as safe microorganism that plays important roles in the biodegradation and production of value-added chemicals. Chromosomal gene deletion of P. putida KT2440 usually involves time-consuming gene coning, conjugal transfer and counterselection. Recently, we developed a P. putida KT2440 markerless gene deletion method based on recombineering and Cre/loxP site-specific recombination. PCR-based λ Red recombineering circumvents the tedious cloning steps and is more amenable to high-throughput manipulation...
October 7, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27736907/scarless-gene-tagging-with-one-step-transformation-and-two-step-selection-in-saccharomyces-cerevisiae-and-schizosaccharomyces-pombe
#10
Dirk Landgraf, Dann Huh, Erinc Hallacli, Susan Lindquist
Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the gene of interest without disrupting regulatory sequences including the 5' and 3' untranslated region (UTR) and without introducing any extraneous unwanted "scar" sequences, which may create unpredictable transcriptional or translational effects. We present a reliable, low-cost, and highly efficient method for the construction of such scarless C-terminal and N-terminal fusions with fluorescent proteins in yeast...
2016: PloS One
https://www.readbyqxmd.com/read/27720988/salt-inducible-protein-splicing-in-cis-and-trans-by-inteins-from-extremely-halophilic-archaea-as-a-novel-protein-engineering-tool
#11
Annika Ciragan, A Sesilja Aranko, Igor Tascon, Hideo Iwaï
Intervening protein sequences (inteins) from extremely halophilic haloarchaea can be inactive under low salinity but could be activated by increasing salt contents to a specific concentration for each intein. The halo-obligatory inteins confer high solubility under both low and high salinity conditions. We showed the broad utility of salt-dependent protein splicing in cis and trans by demonstrating backbone cyclization, self-cleavage for purification, and scarless protein ligation for segmental isotopic labeling...
October 6, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27704196/scarless-epicanthoplasty-and-concomitant-double-eyelidplasty-in-chinese-eyelids
#12
Fufang Ni, Sai Luo, Dongmei Yu, Yangdong Zhu, Yong Shang, Yida Chen, Haiqian Xu, Qing Wang, Lijun Hao
BACKGROUND: The presence of mild or moderate medial epicanthus is typical in Asian patients. Numerous epicanthoplasty techniques have been described previously. However, these methods usually leave obvious scars in the medial canthal area. The aim of this report is to introduce a novel epicanthoplasty technique and a concomitant double eyelidplasty which avoid leaving scars in the medial canthal region. METHODS: From July 2013 to July 2015, 252 patients received epicanthoplasty and concomitant double eyelidplasty with this new technique...
December 2016: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/27686264/single-incision-laparoscopic-appendicectomy-versus-conventional-three-port-laparoscopic-appendicectomy-a-systematic-review-and-meta-analysis
#13
Omar E Aly, Douglas H Black, Haroon Rehman, Irfan Ahmed
BACKGROUND: Appendicectomy is a well-established surgical procedure used in the management of acute appendicitis. The operation can be performed with minimally invasive surgery or as an open procedure. A further development in the minimally invasive appendicectomy technique has been the introduction of single incision laparoscopic surgery (SILA). AIM: To ascertain any differences in outcomes from available trials comparing SILA with conventional multi-incision laparoscopic appendicectomy (CLA)...
October 2, 2016: International Journal of Surgery
https://www.readbyqxmd.com/read/27665369/fibromodulin-is-essential-for-fetal-type-scarless-cutaneous-wound-healing
#14
Zhong Zheng, Xinli Zhang, Catherine Dang, Steven Beanes, Grace X Chang, Yao Chen, Chen-Shuang Li, Kevin S Lee, Kang Ting, Chia Soo
In contrast to adult and late-gestation fetal skin wounds, which heal with scar, early-gestation fetal skin wounds display a remarkable capacity to heal scarlessly. Although the underlying mechanism of this transition from fetal-type scarless healing to adult-type healing with scar has been actively investigated for decades, in utero restoration of scarless healing in late-gestation fetal wounds has not been reported. In this study, using loss- and gain-of-function rodent fetal wound models, we identified that fibromodulin (Fm) is essential for fetal-type scarless wound healing...
November 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27614717/outcome-of-122-delayed-breast-reconstruction-following-post-mastectomy-radiotherapy-the-scarless-latissimus-dorsi-flap-with-tissue-expansion-technique
#15
A de Runz, D Boccara, F Bekara, M Chaouat, M Mimoun
BACKGROUND: Delayed breast reconstruction with tissue expansion may be risky after radiotherapy, due to the poor skin quality. To permit the use of the tissue expansion procedure, we propose a scarless latissimus dorsi flap to bring tissue trophicity, by a healthy vascularized muscular interface with no donor scar and no patch effect. The objective of this study is to assess the outcome of the tissue expansion technique with scarless latissimus dorsi flap after post-mastectomy radiotherapy...
September 7, 2016: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/27574691/-scarless-world-or-scar-less-world-expedition-on-new-perspectives-on-management-of-post-burn-hypertrophic-scar
#16
Cecilia W P Li-Tsang
No abstract text is available yet for this article.
2016: Burns and trauma
https://www.readbyqxmd.com/read/27522509/il-22-mediates-the-oral-mucosal-wound-healing-via-stat3-in-keratinocytes
#17
Ran Yu, Yumei Ding, Lijuan Zhu, Yinying Qu, Chenguang Zhang, Lin Liu, Lili Chen
OBJECTIVE: Wounds are common in the oral cavity. During wound healing, several cytokines are released, which are probably helpful in providing wound debridement, removal of damaged tissues and microbes. Most of the target cells of IL-22 are epithelial cells, which play an important role in mucosa immunity. DESIGN: The function of IL-22 in oral diseases is not well understood. We investigated the expression level of IL-22, collagen I and p-stat3 (Tyr705) via a mice tongue wound model in vivo and detected the effect of IL-22 on the expression of MMP-1, type I collagen and p-stat3 in keratinocytes...
August 2, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27515511/a-programmable-cas9-serine-recombinase-fusion-protein-that-operates-on-dna-sequences-in-mammalian-cells
#18
Brian Chaikind, Jeffrey L Bessen, David B Thompson, Johnny H Hu, David R Liu
We describe the development of 'recCas9', an RNA-programmed small serine recombinase that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic domain of Gin recombinase using an optimized fusion architecture. The resulting recCas9 system recombines DNA sites containing a minimal recombinase core site flanked by guide RNA-specified sequences. We show that these recombinases can operate on DNA sites in mammalian cells identical to genomic loci naturally found in the human genome in a manner that is dependent on the guide RNA sequences...
November 16, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27513730/functional-differences-between-neonatal-and-adult-fibroblasts-and-keratinocytes-donor-age-affects-epithelial-mesenchymal-crosstalk-in-vitro
#19
Rosana Mateu, Veronika Živicová, Eliška Drobná Krejčí, Miloš Grim, Hynek Strnad, Čestmír Vlček, Michal Kolář, Lukáš Lacina, Peter Gál, Jiří Borský, Karel Smetana, Barbora Dvořánková
Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermore, functional assays (proliferation, migration, scratch wound closure) including mutual epithelial‑mesenchymal interactions were also performed to complete the series of experiments. Positivity for nestin and α smooth muscle actin was higher in neonatal fibroblasts (NFs) when compared with their adult counterparts (adult fibroblasts; AFs)...
October 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27510554/the-methylome-and-transcriptome-of-fetal-skin-implications-for-scarless-healing
#20
Justyna Podolak-Popinigis, Anna Ronowicz, Monika Dmochowska, Agnieszka Jakubiak, Paweł Sachadyn
AIM: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition. MATERIALS & METHODS: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach. RESULTS & CONCLUSION: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after...
October 2016: Epigenomics
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