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https://www.readbyqxmd.com/read/29224829/plasticity-of-oral-mucosal-cell-sheets-for-accelerated-and-scarless-skin-wound-healing
#1
Jong-Lyel Roh, Jaewang Lee, Eun Hye Kim, Daiha Shin
OBJECTIVES: Wound healing is generally faster and associated with less scarring in the oral mucosa than in the skin. Although rarely studied, oral mucosa equivalents may contribute to rapid, scarless cutaneous wound healing. Therefore, we examined the potential utility of our newly developed oral mucosal cell sheet in skin wound healing. MATERIALS AND METHODS: Oral mucosa and skin samples were obtained from surgical patients and Sprague-Dawley rats. Keratinocytes and fibroblasts were primarily cultured for in vitro cell expansion...
December 2017: Oral Oncology
https://www.readbyqxmd.com/read/29137618/afeap-cloning-a-precise-and-efficient-method-for-large-dna-sequence-assembly
#2
Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong, Yibin Lin
BACKGROUND: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allows scarless, modular, and reliable construction of biological pathways and circuits from basic genetic parts. METHODS: The AFEAP method requires two-round of PCRs followed by ligation of the sticky ends of DNA fragments...
November 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29118424/methods-for-scarless-selection-free-generation-of-human-cells-and-allele-specific-functional-analysis-of-disease-associated-snps-and-variants-of-uncertain-significance
#3
Nicole B Coggins, Jacob Stultz, Henriette O'Geen, Luis G Carvajal-Carmona, David J Segal
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public health. Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pipeline utilizing KASP (Kompetitive Allele-Specific PCR) genotyping technology to create scarless isogenic cell models of cancer variants in ~1 month. We successfully modeled two novel variants previously identified by our lab in the PALB2 gene in HEK239 cells, resulting in isogenic cells representing all three genotypes for both variants...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29078599/from-full-thoracotomy-to-uniportal-video-assisted-thoracic-surgery-lessons-learned
#4
REVIEW
Eliseo Passera, Gaetano Rocco
Over the last two decades, conventional video-assisted thoracic surgery (VATS) has established itself as the preferred approach for almost all thoracic surgical procedures. The procedure provides a safe and easy approach with undisputed patient benefit at a cost acceptable to the healthcare system all over the world, in large hospitals as well as underprivileged rural areas. VATS has effectively addressed the patients' right to less scarring, trauma (both of access and intrathoracic manipulation), medication, pain, hospitalization, and early return home and work...
2017: Journal of visualized surgery
https://www.readbyqxmd.com/read/29074845/connexin-43-hemichannels-regulate-the-expression-of-wound-healing-associated-genes-in-human-gingival-fibroblasts
#5
Rana Tarzemany, Guoqiao Jiang, Jean X Jiang, Hannu Larjava, Lari Häkkinen
Connexin 43 (Cx43) is the most ubiquitous connexin in various cells, and presents as hemichannels (HCs) and gap junctions (GJs) on the cell membrane. We have recently shown that Cx43 abundance was strongly reduced in fibroblasts of human gingival wounds, and blocking Cx43 function in cultured human gingival fibroblasts (GFBLs) strongly regulated the expression of wound healing-related genes. However, it is not known whether these responses involved Cx43 HCs or GJs. Here we show that Cx43 assembled into distinct GJ and HC plaques in GFBLs both in vivo and in vitro...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29069877/is-robot-assisted-surgery-really-scarless-surgery-immediate-reconstruction-with-a-jejunal-free-flap-for-esophageal-rupture-after-robot-assisted-thyroidectomy
#6
Seong Hoon Park, Joo Hyun Kim, Jun Won Lee, Hii Sun Jeong, Dong Jin Lee, Byung Chun Kim, In Suck Suh
Esophageal perforation is a rare but potentially fatal complication of robot-assisted thyroidectomy (RAT). Herein, we report the long-term outcome of an esophageal reconstruction with a jejunal free flap for esophageal rupture after RAT. A 33-year-old woman developed subcutaneous emphysema and hoarseness on postoperative day1 following RAT. Esophageal rupture was diagnosed by computed tomography and endoscopy, and immediate surgical exploration confirmed esophageal rupture, as well as recurrent laryngeal nerve injury...
November 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/29045533/liposuction-augmentation-mammaplasty
#7
Ryan Harris, Peter Raphael, Scott W Harris
Background: The female breast loses superior fullness and becomes more ptotic over time. Women often present to their plastic surgeon requesting reversal of this evolution. While liposuction alone has been proven to lift the breast, no solution combining augmentation and liposuction has been reported. Objectives: Herein, we introduce a technique called liposuction-augmentation mammaplasty (LAM) that can achieve "scarless" lifting or simply volume equalization prior to inserting same-sized implants...
October 16, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29042658/exosomes-secreted-by-human-adipose-mesenchymal-stem-cells-promote-scarless-cutaneous-repair-by-regulating-extracellular-matrix-remodelling
#8
Lu Wang, Li Hu, Xin Zhou, Zehuan Xiong, Chenguang Zhang, Hassan M A Shehada, Bo Hu, Jinlin Song, Lili Chen
Scar formation is an intractable medical problem that appears after skin wounds have healed. Recent research has shown that exosomes secreted by human adipose mesenchymal stem cells (ASC-Exos) can benefit wound healing. To further explore the therapeutic potential of ASC-Exos, we investigated their effects on mitigating scar formation, and the underlying mechanisms of these effects. We found that intravenous injection of ASC-Exos decreased the size of scars and increased the ratio of collagen III to collagen I in murine incisional wounds...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038745/the-contractile-phenotype-of-dermal-fetal-fibroblasts-in-scarless-wound-healing
#9
Aron Parekh, Patricia A Hebda
PURPOSE OF REVIEW: Injured skin in the mammalian fetus can heal regeneratively due to the ability of fetal fibroblasts to effectively reorganize the extracellular matrix (ECM). This process occurs without fetal fibroblasts differentiating into highly contractile myofibroblasts which cause scarring and fibrosis in adult wounds. Here, we provide a brief review of fetal wound healing and the evidence supporting a unique contractile phenotype in fetal fibroblasts. Furthermore, we discuss the biomechanical role of the ECM in driving myofibroblast differentiation in wound healing and the implications for new clinical modalities based on the biophysical properties of fetal fibroblasts...
September 2017: Current Pathobiology Reports
https://www.readbyqxmd.com/read/29036023/percutaneous-mesh-expansion-pme-a-regenerative-wound-closure-alternative
#10
Kimberly S Khouri, Eufemiano Cardoso, Roger K Khouri
BACKGROUND: Puncture wounds in the 1-mm range usually heal without scars. Stacking rows of these punctures offers a scarless method to generate tissue by mesh expansion. We developed a percutaneous mesh expansion (PME) procedure and present our experience for its wound closure application. METHODS: Over a six-year period we applied PME to 65 consecutive patients 58-101 years-old (mean, 72) with 67 full thickness calvarial defects 2.5x3-7x8cm (mean, 14cm2) that would have all required flaps for closure...
October 12, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28990101/exogenous-peripheral-blood-mononuclear-cells-affect-the-healing-process-of-deep%C3%A2-degree-burns
#11
Guanying Yu, Yaonan Li, Lan Ye, Xinglei Wang, Jixun Zhang, Zhengxue Dong, Duyin Jiang
The regenerative repair of deep‑degree (second degree) burned skin remains a notable challenge in the treatment of burn injury, despite improvements being made with regards to treatment modality and the emergence of novel therapies. Fetal skin constitutes an attractive target for investigating scarless healing of burned skin. To investigate the inflammatory response during scarless healing of burned fetal skin, the present study developed a nude mouse model, which was implanted with normal human fetal skin and burned fetal skin...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28968490/a-novel-tool-for-microbial-genome-editing-using-the-restriction-modification-system
#12
Hua Bai, Aihua Deng, Shuwen Liu, Di Cui, Qidi Qiu, Laiyou Wang, Zhao Yang, Jie Wu, Xiuling Shang, Yun Zhang, Tingyi Wen
Scarless genetic manipulation of genomes is an essential tool for biological research. The restriction-modification (R-M) system is a defense system in bacteria that protects against invading genomes on the basis of its ability to distinguish foreign DNA from self DNA. Here, we designed an R-M system-mediated genome editing (RMGE) technique for scarless genetic manipulation in different microorganisms. For bacteria with Type IV REase, an RMGE technique using the inducible DNA methyltransferase gene, bceSIIM (RMGE-bceSIIM), as the counter-selection cassette was developed to edit the genome of Escherichia coli...
October 12, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28947323/one-trocar-needlescopic-assisted-inguinal-hernia-repair-in-children-a-novel-technique
#13
Rafik Shalaby, Ibrahim Elsayaad, Omar Alsamahy, Refaat Ibrahem, Adham El-Saied, Maged Ismail, Abdelmoniem Shamseldin, Sameh Shehata, Mohamad Magid
BACKGROUND: Inguinal hernia repair using a percutaneous internal ring suturing technique is an effective alternative technique to conventional laparoscopic hernia repair. It is one of the most commonly used approaches for laparoscopic hernia repair in children. However, most percutaneous techniques have utilized extracorporeal knotting of the suture and burying the knot subcutaneously. This approach has several drawbacks. The aim of this study is to present a modified technique for single cannula needlescopic assisted hernia repair in children...
August 31, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28945013/pro-regenerative-hydrogel-restores-scarless-skin-during-cutaneous-wound-healing
#14
Guoming Sun
The transformation of fibrotic healing process to regenerative one has great potential to fully restore wounded skin. The M2 macrophage phenotype promotes constructive tissue remodeling and instructs tissue repair in a regenerative manner. It is hypothesized that hydrogels that can establish robustness of endogenous cells to regulate M2 phenotype will promote constructive dermal remodeling. Toward this end, a series of dextran-based bioabsorbable hydrogels are developed and self-crosslinkable dextran-isocyanatoethyl methacrylate-ethylamine (DexIEME) is identified as the potential scaffold...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28932043/gingival-mesenchymal-stem-cells
#15
Deepak Venkatesh, K P Mohan Kumar, Jyoti B Alur
The human gingiva, characterized by its outstanding scarless wound healing properties, is a unique tissue and a pivotal component of the periodontal apparatus, investing and surrounding the teeth in their sockets in the alveolar bone. In the last year's gingival mesenchymal stem/progenitor cells (GMSCs), with promising regenerative and immunomodulatory properties, have been isolated and characterized from the gingival lamina propria. These cells, in contrast to other mesenchymal stem/progenitor cell (MSC) sources, are abundant, readily accessible and easily obtainable through minimally invasive cell isolation techniques...
May 2017: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/28910979/crispr-cas9-based-efficient-genome-editing-in-staphylococcus-aureus
#16
Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun, Sheng Yang, Daijie Chen
Staphylococcus aureus is an important pathogenic bacterium prevalent in nosocomial infections and associated with high morbidity and mortality rates, which arise from the significant pathogenicity and multi-drug resistance. However, the typical genetic manipulation tools used to explore the relevant molecular mechanisms of S. aureus have multiple limitations: leaving a scar in the genome, comparatively low gene-editing efficiency, and prolonged experimental period. Here, we present a single-plasmid based on the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system which allows rapid and efficient chromosomal manipulation in S...
September 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28901389/analysis-of-dermal-fibroblasts-isolated-from-neonatal-and-child-cleft-lip-and-adult-skin-developmental-implications-on-reconstructive-surgery
#17
Veronika Živicová, Lukáš Lacina, Rosana Mateu, Karel Smetana, Radana Kavková, Eliška Drobná Krejčí, Miloš Grim, Alena Kvasilová, Jiří Borský, Hynek Strnad, Miluše Hradilová, Jana Šáchová, Michal Kolář, Barbora Dvořánková
The nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing of reparative surgery. Based on our previous results on crosstalk between neonatal fibroblasts and adult keratinocytes, the present study focused on characterization of fibroblasts prepared from cleft lip tissue samples of neonates and older children, and compared them with samples isolated from normal adult skin (face and breast) and scars. Although subtle variances in expression profiles of children and neonates were observed, the two groups differed significantly from adult cells...
November 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28898797/scarless-surgery-for-a-huge-liver-cyst-a-case-report
#18
Hiroyuki Kashiwagi, Jun Kawachi, Naoko Isogai, Masanori Ishii, Katsunori Miyake, Rai Shimoyama, Ryota Fukai, Hidemitsu Ogino
INTRODUCTION: Symptomatic or complicated liver cysts sometimes require surgical intervention and laparoscopic fenestration is the definitive treatment for these cysts. We performed minimally invasive surgery, hybrid natural orifice transluminal endoscopic surgery (NOTES) without scarring, for a huge liver cyst. PRESENTATION OF CASE: An 82-year-old female presented with a month-long history of right upper abdominal pain. We diagnosed her condition as a huge liver cyst by morphological studies...
2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28891961/visible-light-cured-glycol-chitosan-hydrogel-containing-a-beta-cyclodextrin-curcumin-inclusion-complex-improves-wound-healing-in-vivo
#19
Sun-Jung Yoon, Hoon Hyun, Deok-Won Lee, Dae Hyeok Yang
Scarless wound healing is ideal for patients suffering from soft tissue defects. In this study, we prepared a novel wet dressing (β-CD-ic-CUR/GC) based on the visible light-cured glycol chitosan (GC) hydrogel and inclusion complex between beta-cyclodextrin (β-CD) and curcumin (CUR). We also evaluated its efficacy in the acceleration of wound healing as compared to that of CUR-loaded GC (CUR/GC). The conjugation of glycidyl methacrylate (GM) to GC for photo-curing was confirmed by ¹H-NMR measurement, and the photo-cured GC hydrogel was characterized by the analyses of rheology, swelling ratio, SEM and degradation rate...
September 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28891857/modified-pull-through-technique-with-a-delayed-high-coloanal-anastomosis-no-stoma-and-scarless-surgery-for-low-rectal-cancer
#20
Francesco Bianco, Armando Falato, Andrea Belli, Silvia De Franciscis, Jesus David De Leon Valdez, Giovanni Maria Romano
No abstract text is available yet for this article.
October 2017: Diseases of the Colon and Rectum
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