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https://www.readbyqxmd.com/read/29789443/low-intensity-pulsed-ultrasound-stimulation-facilitates-in-vitro-osteogenic-differentiation-of-human-adipose-derived-stem-cells-via-up-regulation-of-heat-shock-protein-hsp-70-hsp90-and-bone-morphogenetic-protein-bmp-signaling-pathway
#1
Zhonglei Zhang, Yalin Ma, Shaowen Guo, Yi He, Gang Bai, Wenjun Zhang
Low-intensity pulsed ultrasound (LIPUS) has positive effects on osteogenic differentiation. However, the effect of LIPUS on osteogenic differentiation of human adipose-derived stem cells (hASCs) is unclear. In the present study, we investigated whether LIPUS could promote the proliferation and osteogenic differentiation of hASCs. hASCs were isolated and osteogenically induced with LIPUS stimulation at 20 and 30 mW cm-2 for 30 min day-1 Cell proliferation and osteogenic differentiation potential of hASCs were respectively analyzed by cell counting kit-8 assay, Alizarin Red S staining, real-time polymerase chain reaction, and Western blotting...
May 29, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29785894/developmental-and-molecular-responses-of-buffalo-bubalus-bubalis-cumulus-oocyte-complex-matured-in-vitro-under-heat-shock-conditions
#2
Ashraf El-Sayed, Rehab Nagy, Amal K El-Asheeri, Liala N Eid
SummaryTo investigate the effects of physiologically relevant heat shock during oocyte maturation, buffalo cumulus-oocyte complexes (COCs) were cultured at 38.5°C (control) or were exposed to 39.5°C (T1) or 40.5°C (T2) for the first 6 h of in vitro maturation (IVM), followed by 38.5°C through the next 18 h/IVM and early embryonic development up to the blastocyst stage. Gene expression analysis was performed on selected target genes (HSF-1, HSF-2, HSP-70, HSP-90, BAX, p53, SOD1, COX1, MAPK14) in denuded oocytes and their isolated cumulus cells resulting from control COCs as well as from COCs exposed to a temperature of 39...
May 22, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29775289/mass-spectral-detection-of-diethoxyphospho-tyrosine-adducts-on-proteins-from-hek293-cells-using-monoclonal-antibody-depy-for-enrichment
#3
Seda Onder, Lawrence M Schopfer, Ozden Tacal, Thomas A Blake, Rudolph C Johnson, Oksana Lockridge
Chronic illness from exposure to organophosphorus toxicants is hypothesized to involve modification of unknown proteins. Tyrosine in proteins that have no active site serine readily reacts with organophosphorus toxicants. We developed a monoclonal antibody, depY, that specifically recognizes diethoxyphospho-tyrosine in proteins and peptides, independent of the surrounding amino acid sequence. Our goal in the current study was to identify diethoxyphosphorylated proteins in human HEK293 cell lysate treated with chlorpyrifos oxon...
May 18, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29749547/salidroside-inhibits-the-proliferation-and-migration-of-gastric-cancer-cells-via-suppression-of-src%C3%A2-associated-signaling-pathway-activation-and-heat-shock-protein-70-expression
#4
Zhilin Qi, Tuo Tang, Lili Sheng, Yunfei Ma, Yinhua Liu, Liang Yan, Shimei Qi, Liefeng Ling, Yao Zhang
Salidroside, an active ingredient extracted from the Rhodiola rosea plant, has potential anti‑tumor effects. However, the effects of salidroside on gastric cancer cell proliferation and migration remain unclear. In the present study, the inhibitory effects of salidroside on gastric cancer cell proliferation, migration and invasion and the molecular mechanisms underlying these effects were investigated. The human gastric cancer cell line, BGC‑823, was treated with different concentrations of salidroside (200, 400 and 600 µg/ml)...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29743123/characterization-of-three-heat-shock-protein-70-genes-from-liriomyza-trifolii-and-expression-during-thermal-stress-and-insect-development
#5
Y-W Chang, X-X Zhang, J-Y Chen, M-X Lu, W-R Gong, Y-Z Du
Heat shock proteins (HSPs) participate in diverse physiological processes in insects, and HSP70 is one of the most highly conserved proteins in the HSP family. In this study, full-length cDNAs of three HSP70 genes (Lthsc70, Lthsp701, and Lthsp702) were cloned and characterized from Liriomyza trifolii, an important invasive pest of vegetable crops and horticultural crops worldwide. These three HSP70s exhibited signature sequences and motifs that are typical of the HSP70 family. The expression patterns of the three Lthsp70s during temperature stress and in different insect development stages were studied by real-time quantitative PCR...
May 10, 2018: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29737836/intelligent-mno2-cu2-xs-for-multimode-imaging-diagnostic-and-advanced-single-laser-irradiated-photothermal-photodynamic-therapy
#6
Yu Cao, Xiangdan Meng, Dongdong Wang, Kai Zhang, Wenhao Dai, HaiFeng Dong, Xueji Zhang
Lately, photothermal therapy (PTT) and photodynamic therapy (PDT) dual-modal therapy has attracted much attention in cancer therapy as a synergistic therapeutic model. However, the integration of PDT and PTT in single nanoagent for cancer therapy is still a challenging task. Herein, an intelligent MnO2/Cu2-xS-siRNA nanoagent simultaneously overcoming inherent limitations of PDT and PTT with remarkable PTT/PDT therapeutic efficiency enabling multimode accurate tumor imaging diagnostic is designed. We first develop a general method to decorate Cu2-xS on the surface of MnO2 nanosheet (MnO2/Cu2-xS), then, it was loaded with heat shock protein (HSP) 70 siRNA to obtain MnO2/Cu2-xS-siRNA...
May 8, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29733865/microrna-146-5p-mir-let-7c-5p-mir-221-and-mir-345-5p-are-differentially-expressed-in-respiratory-syncytial-virus-rsv-persistently-infected-hep-2-cells
#7
Eilam-Frenkel B, Naaman H, Berkic G, I Veksler-Lublinsky, G Rall, Shemer Y, Gopas J
Many viruses can establish non-cytolytic, chronic infections in host cells. Beyond the intrinsically interesting questions of how this long-term parasitism is achieved, persistently infected cells can be useful to study virus-host interactions. MicroRNAs (miRNAs) are a class of noncoding RNAs transcribed from the genomes of all multicellular organisms and some viruses. Individual miRNAs may regulate several hundred genes. In this research we have studied the expression of a selective group of host-cell encoded miRNAs, as expressed in a Respiratory Syncytial Virus (RSV) persistently infected HEp-2 cell line (HEp-2+RSV-GFP)...
May 4, 2018: Virus Research
https://www.readbyqxmd.com/read/29733104/fractional-co-2-laser-treatment-for-vaginal-laxity-a-preclinical-study
#8
Tae-Rin Kwon, Jong Hwan Kim, Joon Seok, Jae Min Kim, Dong-Ho Bak, Mi-Ji Choi, Seok Kyun Mun, Chan Woong Kim, Seungwon Ahn, Beom Joon Kim
BACKGROUND AND OBJECTIVE: Various studies have investigated treatment for vaginal laxity with microablative fractional carbon dioxide CO2 laser in humans; however, this treatment has not yet been studied in an animal model. Herein, we evaluate the therapeutic effects of fractional CO2 laser for tissue remodeling of vaginal mucosa using a porcine model, with the aim of improving vaginal laxity. STUDY DESIGN/MATERIALS AND METHODS: The fractional CO2 laser enables minimally invasive and non-incisional procedures...
May 7, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29728821/moringa-oleifera-leaves-extract-protects-titanium-dioxide-nanoparticles-induced-nephrotoxicity-via-nrf2-ho-1-signaling-and-amelioration-of-oxidative-stress
#9
K H Abdou, Walaa A Moselhy, Hanaa M Mohamed, El-Shaymaa El-Nahass, Ahlam G Khalifa
The efficacy of Moringa oleifera leaf extract (MO) in alleviating nephrotoxicity induced by titanium dioxide nanoparticles (TiO2 NPs) was studied. Rats were divided into four groups. Group I received distilled water. Group II received TiO2 NPs. Group III received both TiO2 NPs suspension beside MO. Group IV received MO only. Kidney KIM-1, NF-кB TNF-α, and HSP-70 expression were significantly upregulated while both Nrf2 and HO-1were significantly downregulated in TiO2 NPs-treated rats. MO decreases expression of KIM-1, NF-кB, TNF-α, and HSP-70...
May 4, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29718504/proteomic-based-approach-to-the-proteins-involved-in-1-deoxynojirimycin-accumulation-in-silkworm-bombyx-mori-lepidoptera-bombycidae
#10
Hongxia Chen, Yueqin Liu, Wenbing Wang, Opeyemi J Olatunji, Gang Pan, Zhen Ouyang
1-Deoxynojirimycin (DNJ) is the most abundant poly-hydroxylated alkaloid in the latex of mulberry leaves and it protects mulberry from insect predation. However, silkworms can survive the poisoning effect of DNJ and accumulate DNJ by consumption of the mulberry leaves. In order to determine the molecular mechanism of DNJ accumulation in silkworm, comparative proteomic analysis was employed to evaluate protein expression in two groups of silkworm bodies (the third instar silkworm bodies had the maximum content of DNJ throughout life, and the newly hatched silkworm bodies had no DNJ)...
March 1, 2018: Journal of Insect Science
https://www.readbyqxmd.com/read/29715465/up-regulation-of-mir-122-protects-against-neuronal-cell-death-in-ischemic-stroke-through-the-heat-shock-protein-70-dependent-nf-%C3%AE%C2%BAb-pathway-by-targeting-foxo3
#11
Dong Guo, Ji Ma, Tengfei Li, Lei Yan
Dysfunction of the microRNA (miR) network has been emerging as a main regulator in ischemic stroke. Recently, studies have linked the deregulation of miR-122 to ischemic stroke. However, the specific role and molecular mechanism of miR-122 in ischemic stroke remain to be further investigated. Here, we found that miR-122 was decreased in mouse N2A neuroblastoma (N2A) cells after oxygen-glucose deprivation (OGD) and mouse brain after transient middle cerebral artery occlusion (MCAO). OGD treatment significantly increased N2A cell death and Caspase-3 activity, and decreased Bcl-2 protein expression...
April 28, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29679484/the-role-of-autoimmune-reactivity-induced-by-heat-shock-protein-70-in-the-pathogenesis-of-essential-hypertension
#12
REVIEW
Bernardo Rodriguez-Iturbe, Miguel A Lanaspa, Richard J Johnson
Autoimmunity is increasingly recognized as having a central role in essential hypertension. Heat shock proteins are immunodominant molecules with high interspecies homology and autoimmune reactivity directed against HSP70 may play a role in the pathogenesis of hypertension. Autoimmunity to HSP70 may result from molecular mimicry between human HSP and bacterial HSP, or alternatively, as a response to HSP70-peptide complexes generated during cellular stress and delivered to the major histocompatibility complex in antigen presenting cells...
April 21, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29675023/increased-plasma-levels-of-danger-associated-molecular-patterns-are-associated-with-immune-suppression-and-postoperative-infections-in-patients-undergoing-cytoreductive-surgery-and-hyperthermic-intraperitoneal-chemotherapy
#13
Guus P Leijte, Hettie Custers, Jelle Gerretsen, Amon Heijne, Johannes Roth, Thomas Vogl, Gert J Scheffer, Peter Pickkers, Matthijs Kox
Introduction: Danger-associated molecular patterns (DAMPs) can elicit immune responses and may subsequently induce an immune-suppressed state. Previous work showed that increased plasma levels of DAMPs are associated with immune suppression and increased susceptibility toward infections in trauma patients. Like trauma, major surgical procedures, such as cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC), are also thought to cause profound DAMP release...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29622083/role-of-drug-transporters-and-heat-shock-proteins-during-ethanol-exposure-to-human-neural-precursor-cells-and-its-lineages
#14
Sandeep Kumar Vishwakarma, Nusrath Fatima, Chandrakala Lakkireddy, Nagarapu Raju, Avinash Bardia, A Sandhya, Syed Ameer Basha Paspala, Vishnupriya Satti, Aleem Ahmed Khan
INTRODUCTION: Ethanol exposure to developing brain may alter the growth and differentiation of neurological cells resulting in unfavorable pathologies. Earlier studies have provided very limited mechanistic insights of cellular and molecular mechanisms which do not mimic with human situation due to varying cell types and poses potential challenges for investigation. Therefore, the present study was undertaken to evaluate the role of ABC transporters and heat shock proteins mediated response in human neural precursor cells (NPCs) and its lineages during proliferation and lineage differentiation against ethanol exposure...
April 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29621162/toxic-effects-of-bisphenol-a-propyl-paraben-and-triclosan-on-caenorhabditis-elegans
#15
María Cecilia García-Espiñeira, Lesly Patricia Tejeda-Benítez, Jesus Olivero-Verbel
Bisphenol A (BPA) is a ubiquitous plasticizer which is absorbed by ingestion and dermal contact; propyl paraben (PPB) inhibits the microbiome and extends the shelf life of many personal care products, whereas triclosan (TCS) is commonly found in antiseptics, disinfectants, or additives. In this work, Caenorhabditis elegans was used as a biological model to assess the toxic effects of BPA, PPB, and TCS. The wild type strain, Bristol N2, was used in bioassays with the endpoints of lethality, growth, and reproduction; green fluorescent protein (GFP) transgenic strains with the hsp-3 , hsp-4 , hsp-16...
April 5, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29604402/effects-of-aquatic-and-land-based-exercises-on-amyloid-beta-heat-shock-protein-27-and-pulse-wave-velocity-in-elderly-women
#16
Ji-Hyeon Kim, Young-Suk Jung, Jong-Won Kim, Min-Seong Ha, Soo-Min Ha, Do-Yeon Kim
BACKGROUND: Alzheimer's disease is a neurodegenerative brain disease resulting from the deterioration of neuronal cells and vascular dementia, the latter of which results from cerebrovascular disorders. Exercise is effective in preventing and treating degenerative brain diseases as it activates blood flow to the brain, increases nerve production in the hippocampus, and promotes the expression of synaptic plasticity-related proteins. Therefore, this study investigated the effects of 16-week aquatic and land-based exercise programs on amyloid beta (Aβ), heat shock protein (HSP) 27 levels, and pulse wave velocity (PWV)...
March 28, 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29568083/identification-of-highly-effective-target-genes-for-rnai-mediated-control-of-emerald-ash-borer-agrilus-planipennis
#17
Thais B Rodrigues, Jian J Duan, Subba R Palli, Lynne K Rieske
Recent study has shown that RNA interference (RNAi) is efficient in emerald ash borer (EAB), Agrilus planipennis, and that ingestion of double-stranded RNA (dsRNA) targeting specific genes causes gene silencing and mortality in neonates. Here, we report on the identification of highly effective target genes for RNAi-mediated control of EAB. We screened 13 candidate genes in neonate larvae and selected the most effective target genes for further investigation, including their effect on EAB adults and on a non-target organism, Tribolium castaneum...
March 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29561941/analyzing-the-neuropsychological-characteristics-and-changes-in-serum-markers-of-patients-with-chronic-cerebral-circulation-insufficiency
#18
Jianhua Tang, Yuqing Zhen, Ling Yu, Cui Lv, Juan Zheng, Hui Liang
OBJECTIVE: To investigate the neuropsychological characteristics and changes in CRP, S100B, MBP, HSP-7, and NSE in serum. METHOD: Sixty-six (66) patients treated in our hospital as CCCI group were chosen for our study, and 90 patients with depression were selected as the depression group. The patients in both groups were examined with CT perfusion, depression, anxiety and cognition evaluation. Their serum CRP, S100B, MBP, HSP-70 and NSE levels were detected. Neuropsychological and serum markers characteristics were compared...
January 2018: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/29541984/effects-of-silver-nanoparticles-on-stress-biomarkers-of-channa-striatus-immuno-protective-or-toxic
#19
Neeraj Kumar, Kishore Kumar Krishnani, Sanjay Kumar Gupta, Narendra Pratap Singh
Nanotechnology is a novel arena with promising applications in the field of medicine, industry, and agriculture including fisheries. Cross-disciplinary interactions and the application of this technology in biological systems have led to the innovation of novel nanoparticle antioxidants, which are the subject of our study. In context with above background, we designed an experiment on nano-silver to elucidate its role for mitigation of abiotic and biotic stress. Three diets were formulated viz. silver nanoparticles (Ag-NPs) incorporated at 0...
March 14, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29528531/a-novel-heterozygous-variant-in-erlin2-causes-autosomal-dominant-pure-hereditary-spastic-paraplegia
#20
S L Rydning, A Dudesek, F Rimmele, C Funke, S Krüger, S Biskup, M D Vigeland, H S Hjorthaug, Y Sejersted, C Tallaksen, K K Selmer, C Kamm
BACKGROUND AND PURPOSE: Hereditary spastic paraplegias (HSPs) are clinically and genetically heterogeneous monogenic disorders. To date, nearly 70 genes are known to be causative. The aim of this project was to identify the genetic cause of autosomal dominantly inherited pure HSP in two large, unrelated non-consanguineous families. METHODS: The two families were characterized clinically and selected members underwent whole exome sequencing. Potentially disease-causing variants were confirmed by Sanger sequencing and their functional consequences on protein function were predicted by bioinformatic prediction tools...
March 12, 2018: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
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