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https://www.readbyqxmd.com/read/27916788/effects-of-dietary-oregano-essential-oil-and-vitamin-e-supplementation-on-meat-quality-stress-response-and-intestinal-morphology-in-pigs-following-transport-stress
#1
Yi Zou, Xiao Ming Hu, Ting Zhang, Hong Kui Wei, Yuan Fei Zhou, Zhong Xin Zhou, Jian Peng
This study investigates the effects of dietary oregano essential oil (OEO) and vitamin E (Vit E) supplementation on meat quality, stress response and intestinal morphology in pigs following transport stress. A total of 288 finishing pigs were randomly assigned to three groups: a basal diet or a basal diet supplemented either with 200 mg/kg Vit E or 25 mg/kg OEO. After a 28-day feeding trial, total of 132 finishing pigs according diet and transport stress were assigned to one of four treatment groups: 1) control treatment without transport stress (Control group), 2) control treatment with 5-hr transport stress (Negative group), 3) Vit E treatment with 5-hr transport stress and 4) OEO treatment with 5-hr transport stress...
December 2, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27916661/tetrameric-assembly-of-k-channels-requires-er-located-chaperone-proteins
#2
Kai Li, Qiang Jiang, Xue Bai, Yi-Feng Yang, Mei-Yu Ruan, Shi-Qing Cai
Tetrameric assembly of channel subunits in the endoplasmic reticulum (ER) is essential for surface expression and function of K(+) channels, but the molecular mechanism underlying this process remains unclear. In this study, we found through genetic screening that ER-located J-domain-containing chaperone proteins (J-proteins) are critical for the biogenesis and physiological function of ether-a-go-go-related gene (ERG) K(+) channels in both Caenorhabditis elegans and human cells. Human J-proteins DNAJB12 and DNAJB14 promoted tetrameric assembly of ERG (and Kv4...
November 30, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27908578/water-pollution-causes-ultrastructural-and-functional-damages-in-pellia-neesiana-gottsche-limpr
#3
Adriana Basile, Sergio Sorbo, Marco Lentini, Barbara Conte, Sergio Esposito
The aim of this work is to evaluate the effects of freshwater pollution in the heavily contaminated Sarno River (Campania, South Italy), using Pellia neesiana (Pelliaceae Metzgeriales) in order to propose this liverwort as a potential bioindicator, able to record the effects of water pollution, particularly the one related to metal (loid) contamination. Samples of P. neesiana in nylon bags were disposed floating for one week on the waters of Sarno River in three sites characterised by an increasing pollution...
November 25, 2016: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27902749/risk-factors-associated-with-renal-involvement-in-childhood-henoch-sch%C3%A3-nlein-purpura-a-meta-analysis
#4
Han Chan, Yan-Ling Tang, Xiao-Hang Lv, Gao-Fu Zhang, Mo Wang, Hai-Ping Yang, Qiu Li
BACKGROUND AND OBJECTIVE: Henoch-Schönlein purpura (HSP) is an important cause of chronic kidney disease in children. This meta-analysis identified risk factors associated with renal involvement in childhood HSP. METHODS: PubMed, Embase, and Web of Science were searched. The quality of all eligible studies was assessed using the Newcastle-Ottawa scale criteria. An analysis of possible risk factors was conducted to report the odds ratio (OR) and weighted mean difference (WMD)...
2016: PloS One
https://www.readbyqxmd.com/read/27900999/evaluating-anti-smd1-amino-acid-83-119-peptide-reactivity-in-children-with-systemic-lupus-erythematosus-and-other-immunological-diseases
#5
Hai-Ou Yang, Xiao-Qing Zhang, Qi-Hua Fu
BACKGROUND: SmD1-amino-acid 83-119 peptide (SmD183-119) is the major epitope of Smith (Sm) antigen, which is specific for adult systemic lupus erythematosus (SLE). The anti-SmD183-119 antibody has exhibited higher sensitivity and specificity than anti-Sm antibody in diagnosing adult SLE. However, the utility of anti-SmD183-119antibodies remains unclear in children with SLE (cSLE). This study aimed to assess the characteristics of anti-SmD183-119antibody in the diagnosis of cSLE. METHODS: Samples from 242 children with different rheumatological and immunological disorders, including autoimmune diseases (SLE [n = 46] and ankylosing spondylitis [AS, n = 11]), nonautoimmune diseases (Henoch-Schonlein purpura [HSP, n = 60], idiopathic thrombocytopenia purpura [n = 27], hematuria [n = 59], and arthralgia [n = 39]) were collected from Shanghai Children's Medical Center from March 6, 2012 to February 27, 2014...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27900018/influence-of-ezrin-shrna-in-combination-with-hsp70-on-the-apoptosis-and-proliferation-of-osteosarcoma-cells
#6
Qin Yao, Yihua Pei, Huiqin Zhuo, Bozhen Xie
Ezrin and heat shock protein (HSP)70 have been reported to regulate cell apoptosis and tumor development of osteosarcoma. However, there has not been reported the synergy effect of knocking down ezrin and overexpressing HSP70. In the present study, two vectors, pGFP-V-RS-shRNA and pGFP-V-RS-shRNA-HSP70, were constructed and transfected into LM8 cells [denoted as small hairpin (sh)RNA group and dual group, respectively]. The apoptosis rates in these two transfected groups were significantly higher than those in the control group (empty vector) (P=0...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27879375/the-role-of-oxidative-stress-in-koenimbine-induced-dna-damage-and-heat-shock-protein-modulation-in-hepg2-cells
#7
Yahya Hasan Hobani
Murraya koenigii (L.) Spreng, is a significant herb of traditional Ayurvedic system of medicine. Koenimbine, a carbazole alkaloid isolated from this plant holds antiproliferative and apoptotic effects. The aim of this study was to assess koenimbine-induced DNA damage and to clarify the role of free radicals in cell death mechanisms, using HepG2 cells. Methods The level of cytotoxicity was assayed by MTT assay. To elucidate the role of glutathione (GSH), the intracellular GSH level was analyzed. The effect of koenimbine in the cell mitochondria was evaluated using mitochondrial membrane potential (MMP) changes...
November 21, 2016: Integrative Cancer Therapies
https://www.readbyqxmd.com/read/27830002/comparative-analysis-of-the-interaction-of-hsps-in-dendritic-cells-macrophages-rgm-1-cells-infected-by-helicobacter-pylori
#8
Yongliang Yao, Jianhong Wu, Tao Gu, Yang Cheng, Guangxin Li
Helicobacter pylori may cause chronic gastritis, even gastric cancer, however, antigen-presenting cells (APCs) are most important immune cells involved in the induction and expression of the underlying inflammatory responses to resist H. pylori. To study the interaction of HSPs in dendritic cells (DCs), macrophages and RGM-1 cells infected with H. pylori, HSP-27, HSP-60, HSP-70 and HSP-90 proteins were analyzed in the mucosa tissue or serum of gastritis patients caused by H. pylori, and in cell supernatant of DCs, macrophages, RGM-1 infected by H...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27829973/common-functions-of-the-chloroplast-and-mitochondrial-co-chaperones-cpdnajl-cdf1-and-mtdnaj-pam16-in-protein-import-and-ros-scavenging-in-arabidopsis-thaliana
#9
REVIEW
John Gray, Sachin Rustgi, Diter von Wettstein, Christiane Reinbothe, Steffen Reinbothe
As semi-autonomous cell organelles that contain only limited coding information in their own DNA, chloroplasts and mitochondria must import the vast majority of their protein constituents from the cytosol. Respective protein import machineries have been identified that mediate the uptake of chloroplast and mitochondrial proteins and interact with molecular chaperones of the HEAT-SHOCK PROTEIN (HSP) 70 family operating as import motors. Recent work identified unexpected new functions of 2 DnaJ co-chaperones in mitochondrial and chloroplast protein translocation and suggest a common mechanism of reactive oxygen species (ROS) scavenging that shall be discussed here...
2016: Communicative & Integrative Biology
https://www.readbyqxmd.com/read/27825870/short-term-molecular-and-physiological-responses-to-heat-stress-in-neritic-copepods-acartia-tonsa-and-eurytemora-affinis
#10
Janina Rahlff, Janna Peters, Marta Moyano, Ole Pless, Carsten Claussen, Myron A Peck
Invertebrates inhabiting shallow water habitats represent particularly appropriate organisms for studying the acclimation potential to environmental stress, since they naturally experience large fluctuations in key abiotic factors such as temperature and salinity. We quantified the biochemical- (mRNA transcripts of 78-kDa glucose-regulated protein (grp78), 70-kDa heat shock protein (hsp70), 90-kDa heat shock protein (hsp90), protein synthesis of HSP70) and organismal- (oxygen consumption rates) level responses to acute heat stress on two neritic copepods (Acartia tonsa and Eurytemora affinis) with special emphasis on the role of short-term acclimation...
November 5, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27802836/involvement-of-heat-shock-proteins-on-mn-induced-toxicity-in-caenorhabditis-elegans
#11
Daiana Silva Avila, Alexandre Benedetto, Catherine Au, Julia Bornhorst, Michael Aschner
BACKGROUND: All living cells display a rapid molecular response to adverse environmental conditions, and the heat shock protein family reflects one such example. Hence, failing to activate heat shock proteins can impair the cellular response. In the present study, we evaluated whether the loss of different isoforms of heat shock protein (hsp) genes in Caenorhabditis elegans would affect their vulnerability to Manganese (Mn) toxicity. METHODS: We exposed wild type and selected hsp mutant worms to Mn (30 min) and next evaluated further the most susceptible strains...
November 2, 2016: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27799162/protein-stabilization-improves-stat3-function-in-autosomal-dominant-hyper-ige-syndrome
#12
Claire E Bocchini, Karen Nahmod, Panagiotis Katsonis, Sang Kim, Moses M Kasembeli, Alexandra Freeman, Olivier Lichtarge, George Makedonas, David J Tweardy
AD-HIES is caused by dominant negative mutations in STAT3; however, the molecular basis for mutant STAT3 allele dysfunction is unclear and treatment remains supportive. We hypothesized that AD-HIES mutations decrease STAT3 protein stability and that mutant STAT3 activity can be improved by agents that increase chaperone protein activity. We used computer modeling to characterize the effect of STAT3 mutations on protein stability. We measured STAT3 protein half-life (t1/2) and determined levels of STAT3 phosphorylated on tyrosine (Y) 705 (pY-STAT3) and mRNA levels of STAT3 gene targets in EBV-transformed B (EBV) cells, human peripheral blood mononuclear cells (PBMC), and mouse splenocytes incubated without or with chaperone protein modulators-HSF1A, a small-molecule TRiC modulator, or geranylgeranylacetone (GGA), a drug that upregulates heat shock protein (HSP) 70 and HSP90...
October 31, 2016: Blood
https://www.readbyqxmd.com/read/27789295/effect-of-stress-on-heat-shock-protein-levels-immune-response-and-survival-to-fungal-infection-of-mamestra-brassicae-larvae
#13
E H Richards, M P Dani, Y Lu, T Butt, R J Weaver
Although the utilisation of fungal biological control agents to kill insect pests is desirable, it is known that the outcome of infection may be influenced by a number of criteria, including whether or not the target insect is stressed. In the current work, topical treatment of larvae of the lepidopteran pest, Mamestra brassicae, with conidia of Beauveria bassiana, followed by a heat stress (HS; 37°C for 1h) 48h later, resulted in a similar level of larval survival to that occurring for no heat stress (No-HS), fungus-treated larvae...
October 24, 2016: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27771117/cellular-thermotolerance-is-inheritable-from-holstein-cattle-cloned-with-ooplasts-of-taiwan-native-yellow-cattle
#14
Piyawit Kesorn, Jai-Wei Lee, Hung-Yi Wu, Jyh-Cherng Ju, Shao-Yu Peng, Shyh-Shyan Liu, Hsi-Hsun Wu, Perng-Chih Shen
We have previously demonstrated that the somatic cells from cattle cloned with Holstein (H) donor cells and Taiwan native yellow cattle (Y) ooplasm (Yo-Hd) had better thermotolerance than those from cattle cloned with both Holstein donor cells and ooplasm (Ho-Hd). The present study aimed to investigate whether the cellular thermotolerance of these cloned cattle is transmissible to their offspring (Ho-Hd-F1 and Yo-Hd-F1). Thermotolerance of ear fibroblasts derived from these cloned cattle and their offspring were analyzed...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27769261/steroid-resistance-in-copd-is-associated-with-impaired-molecular-chaperone-hsp90-expression-by-pro-inflammatory-lymphocytes
#15
Greg Hodge, Eugene Roscioli, Hubertus Jersmann, Hai B Tran, Mark Holmes, Paul N Reynolds, Sandra Hodge
BACKGROUND: Corticosteroid resistance is a major barrier to effective treatment of COPD. We have shown that the resistance is associated with decreased expression of glucocorticoid receptor (GCR) by senescent CD28nullCD8+ pro-inflammatory lymphocytes in peripheral blood of COPD patients. GCR must be bound to molecular chaperones heat shock proteins (Hsp) 70 and Hsp90 to acquire a high-affinity steroid binding conformation, and traffic to the nucleus. We hypothesized a loss of Hsp70/90 from these lymphocytes may further contribute to steroid resistance in COPD...
October 21, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27737678/effect-of-helium-pre-or-postconditioning-on-signal-transduction-kinases-in-patients-undergoing-coronary-artery-bypass-graft-surgery
#16
Kirsten F Smit, Daniel Brevoord, Stefan De Hert, Bas A de Mol, Raphaela P Kerindongo, Susan van Dieren, Wolfgang S Schlack, Markus W Hollmann, Nina C Weber, Benedikt Preckel
BACKGROUND: The noble gas helium induces pre- and postconditioning in animals and humans. Volatile anesthetics induce cardioprotection in humans undergoing coronary artery bypass graft (CABG) surgery. We hypothesized that helium induces pre- and postconditioning in CABG-patients, affecting signaling molecules protein kinase C-epsilon (PKC-ε), p38 mitogen activated protein kinase (p38 MAPK), extracellular signal-regulated kinase 1/2 (ERK-1/2) and heat shock protein 27 (HSP-27) within cardiac tissue, and reducing postoperative troponin levels...
October 14, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27708256/ard1-mediated-hsp70-acetylation-balances-stress-induced-protein-refolding-and-degradation
#17
Ji Hae Seo, Ji-Hyeon Park, Eun Ji Lee, Tam Thuy Lu Vo, Hoon Choi, Jun Yong Kim, Jae Kyung Jang, Hee-Jun Wee, Hye Shin Lee, Se Hwan Jang, Zee Yong Park, Jaeho Jeong, Kong-Joo Lee, Seung-Hyeon Seok, Jin Young Park, Bong Jin Lee, Mi-Ni Lee, Goo Taeg Oh, Kyu-Won Kim
Heat shock protein (Hsp)70 is a molecular chaperone that maintains protein homoeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. However, the mechanisms by which Hsp70 balances these opposing functions under stress conditions remain unknown. Here, we demonstrate that Hsp70 preferentially facilitates protein refolding after stress, gradually switching to protein degradation via a mechanism dependent on ARD1-mediated Hsp70 acetylation. During the early stress response, Hsp70 is immediately acetylated by ARD1 at K77, and the acetylated Hsp70 binds to the co-chaperone Hop to allow protein refolding...
October 6, 2016: Nature Communications
https://www.readbyqxmd.com/read/27706716/expression-patterns-of-two-heat-shock-cognate-70-genes-during-immune-responses-and-larval-development-of-the-chinese-mitten-crab-eriocheir-sinensis
#18
P Li, X F Jiang, W B Guo, J Yan, K Y Zhou
Two heat-shock protein (HSP) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as EsHSC70-5 and EsHSC70-3, respectively), were isolated from the Chinese mitten crab Eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adult crabs. The HSPs exhibited 45-89% identity with other heat-shock proteins, and they shared similar structural features. EsHSC70 mRNA expression was detected not only during infection but also during the developmental larval stages...
September 16, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27701059/exploring-hazards-of-acute-exposure-of-acephate-in-drosophila-melanogaster-and-search-for-l-ascorbic-acid-mediated-defense-in-it
#19
Prem Rajak, Moumita Dutta, Salma Khatun, Moutushi Mandi, Sumedha Roy
This study reveals protective role of l-ascorbic acid (25, 50 and 100μg/mL) against toxic impacts of acute sub-lethal exposure of Acephate (5μg/mL) in a non-target organism Drosophila melanogaster. Organismal effect was evident from increased impairment in climbing activities (9 folds) of treated individuals who also manifested altered ocular architecture. These anomalies were reduced with l-ascorbic acid (l-AA) supplementation. Acephate induced apoptotic lesions in eye imaginal discs and gut confirmed tissue damage that also reduced with l-AA co-treatment...
September 28, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27696080/determining-protein-biomarkers-for-dlbcl-using-ffpe-tissues-from-hiv-negative-and-hiv-positive-patients
#20
Pumza Magangane, Raveendra Sookhayi, Dhirendra Govender, Richard Naidoo
DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and specificity. In addition, these biomarkers have not been studied in the HIV context. Therefore, the identification of new biomarkers for HIV negative and HIV positive DLBCL, may lead to a better understanding of the disease pathology and better therapeutic design...
October 1, 2016: Journal of Molecular Histology
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