Read by QxMD icon Read


Xiaoye Liu, Shuangyang Ding, Peijie Shi, Richard Dietrich, Erwin Märtlbauer, Kui Zhu
Bacillus cereus is an opportunistic pathogen that often causes foodborne infectious diseases and food poisoning. Non-haemolytic enterotoxin (Nhe) is the major toxin found in almost all enteropathogenic B. cereus and B. thuringiensis isolates. However, little is known about the cellular response after Nhe triggered pore formation on cell membrane. Here we demonstrate that Nhe induced cell cycle arrest at G0 /G1 phase and provoked apoptosis in Vero cells, most likely associated with mitogen-activated protein kinase (MAPK) and death receptor pathways...
October 20, 2016: Cellular Microbiology
Min Ji Bak, Van-Long Truong, Se-Yeon Ko, Xuan Ngan Giang Nguyen, Mira Jun, Soon-Gi Hong, Jong-Won Lee, Woo-Sik Jeong
BACKGROUND: The induction of cellular defensive genes such as phase II detoxifying and antioxidant enzymes is a highly effective strategy for protection against carcinogenesis as well as slowing cancer development. Transcription factor Nrf2 (nuclear factor E2-related factor 2) is responsible for activation of phase II enzymes induced by natural chemopreventive compounds. METHODS: Red ginseng oil (RGO) was extracted using a supercritical CO2 extraction system and chemical profile of RGO was investigated by GC/MS...
October 2016: Journal of Ginseng Research
Er-Wen Huang, Can-Zhao Liu, Si-Jia Liang, Zheng Zhang, Xiao-Fei Lv, Jie Liu, Jia-Guo Zhou, Yong-Bo Tang, Yong-Yuan Guan
BACKGROUND AND AIMS: Macrophage-derived foam cell formation (MFCF) is a crucial step in the pathogenesis of atherosclerosis. Uptake of oxidized low-density lipoprotein (oxLDL) by scavenger receptors is indispensable for MFCF. Endophilin-A2 has been reported to regulate clathrin-mediated endocytosis (CME). In this study, we tested the hypothesis that endophilin-A2 regulates oxLDL uptake and MFCF by mediating CME of oxLDL-scavenger receptor complexes. METHODS: In vitro MFCF was induced by oxLDL treatment...
October 6, 2016: Atherosclerosis
Youguang Luo, Jie Ran, Songbo Xie, Yunfan Yang, Jie Chen, Shanshan Li, Wenqing Shui, Dengwen Li, Min Liu, Jun Zhou
Orientation and positioning of the mitotic spindle are involved in dictating cell division axis and cleavage site, and play important roles in cell fate determination and tissue morphogenesis. However, how spindle movement is controlled to achieve a defined alignment within the dividing cell is not fully understood. Here, we describe an unexpected role for apoptosis signal-regulating kinase 1 (ASK1) in regulating spindle behavior. We find that ASK1 is required for proper mitotic progression and daughter cell adhesion to the substratum...
2016: Cell Discovery
Anna Ju, Young-Chang Cho, Ba Reum Kim, Sung Goo Park, Jeong-Hoon Kim, Kwonseop Kim, Jaehwi Lee, Byoung Chul Park, Sayeon Cho
Mitogen-activated protein kinases (MAPKs) are involved in a variety of intracellular events such as gene expression, cell proliferation, and programmed cell death. MAPKs are activated by dual phosphorylation on threonine and tyrosine residues through sequential activation of protein kinases. Recent studies have shown that the protein kinases involved in MAPK signal transductions might be organized into signaling complexes by scaffold proteins. These scaffold proteins are essential regulators that function by assembling the relevant molecular components in mammalian cells...
2016: PloS One
Chihiro Sakauchi, Hiroaki Wakatsuki, Hidenori Ichijo, Kazuki Hattori
BACKGROUND: Apoptosis signal-regulating kinase 1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5), has the potential to induce cellular apoptosis under various physiological conditions. It has long been suggested that ASK1 is highly sensitive to oxidative stress and contributes substantially to apoptosis. However, recent studies have indicated that ASK1 has pleiotropic roles in living organisms through other mechanisms in addition to apoptosis. SCOPE OF THE REVIEW: This review describes the physiological functions of ASK1 in living organisms, focusing on the regulatory mechanisms of ASK1 activity and its importance in the pathogenesis of various diseases...
September 30, 2016: Biochimica et Biophysica Acta
So Y Cheon, Kyoung J Cho, So Y Kim, Eun H Kam, Jong E Lee, Bon-Nyeo Koo
Conditions of increased oxidative stress including cerebral ischemia can lead to blood-brain barrier dysfunction via matrix metalloproteinase (MMP). It is known that MMP-9 in particular is released from brain endothelial cells is involved in the neuronal cell death that occurs after cerebral ischemia. In the intracellular signaling network, apoptosis signal-regulating kinase 1 (ASK1) is the main activator of the oxidative stress that is part of the pathogenesis of cerebral ischemia. ASK1 also promotes apoptotic cell death and brain infarction after ischemia and is associated with vascular permeability and the formation of brain edema...
2016: Frontiers in Cellular Neuroscience
Namiko Yamaguchi, Shiori Sekine, Isao Naguro, Yusuke Sekine, Hidenori Ichijo
Systemic inflammatory response syndrome (SIRS) is a form of fatal acute inflammation for which there is no effective treatment. Here, we revealed that the ablation of Kelch domain containing 10 (KLHDC10), which we had originally identified as an activator of Apoptosis Signal-regulating Kinase 1 (ASK1), protects mice against TNFα-induced SIRS. The disease development of SIRS is mainly divided into two stages. The early stage is characterized by TNFα-induced systemic necroptosis, a regulated form of necrosis mediated by Receptor-interacting protein (RIP) 1/3 kinases...
2016: PloS One
Atsuko Kimura, Kazuhiko Namekata, Xiaoli Guo, Chikako Harada, Takayuki Harada
Glaucoma is a neurodegenerative disease of the eye and it is one of the major causes of blindness. Glaucoma is usually associated with elevated intraocular pressure (IOP) and the current therapy focuses on reduction of IOP. However, neuroprotective strategies could also be beneficial for treatment of glaucoma because the pathology of the disease involves retinal ganglion cell (RGC) death and damage to the optic nerve. Dedicator of cytokinesis 3 (Dock3) is an atypical guanine exchange factor (GEF) that belongs to a family of Dock proteins, Dock1-11...
September 9, 2016: Histology and Histopathology
Fuminori Yamaguchi, Mitsumasa Tsuchiya, Seiko Shimamoto, Tomohito Fujimoto, Hiroshi Tokumitsu, Masaaki Tokuda, Ryoji Kobayashi
Oxidative stress is the consequence of an imbalance between the production of harmful reactive oxygen species and the cellular antioxidant system for neutralization, and it activates multiple intracellular signaling pathways, including apoptosis signal-regulating kinase 1 (ASK1). Protein phosphatase 5 (PP5) is a serine/threonine phosphatase involved in oxidative stress responses. Previously, we reported that S100 proteins activate PP5 in a calcium-dependent manner. S100 proteins belong to a family of small EF-hand calcium-binding proteins involved in many processes such as cell proliferation, differentiation, apoptosis, and inflammation...
2016: Tohoku Journal of Experimental Medicine
Salome Kylarova, Dalibor Kosek, Olivia Petrvalska, Katarina Psenakova, Petr Man, Jaroslav Vecer, Petr Herman, Veronika Obsilova, Tomas Obsil
Apoptosis signal-regulating kinase 1 (ASK1, MAP3K5) activates p38 mitogen-activated protein kinase and the c-Jun N-terminal kinase in response to proinflammatory and stress signals. In non-stress conditions, ASK1 is inhibited by association with thioredoxin (TRX) which binds to the TRX-binding domain (ASK1-TBD) at the N-terminus of ASK1. TRX dissociates in response to oxidative stress allowing the ASK1 activation. However, the molecular basis for the ASK1:TRX1 complex dissociation is still not fully understood...
September 2, 2016: FEBS Journal
Olivia Petrvalska, Dalibor Kosek, Zdenek Kukacka, Zdenek Tosner, Petr Man, Jaroslav Vecer, Petr Herman, Veronika Obsilova, Tomas Obsil
Apoptosis signal-regulating kinase 1 (ASK1, also known as MAP3K5), a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, regulates diverse physiological processes. The activity of ASK1 is triggered by various stress stimuli and is involved in the pathogenesis of cancer, neurodegeneration, inflammation, and diabetes. ASK1 forms a high molecular mass complex whose activity is, under non-stress conditions, suppressed through interaction with thioredoxin and the scaffolding protein 14-3-3...
September 23, 2016: Journal of Biological Chemistry
Stefan Donath, Junfeng An, Sabrina Lin Lin Lee, Karen Gertz, Anna Lena Datwyler, Ulrike Harms, Susanne Müller, Tracy Deanne Farr, Martina Füchtemeier, Gisela Lättig-Tünnemann, Janet Lips, Marco Foddis, Larissa Mosch, René Bernard, Ulrike Grittner, Mustafa Balkaya, Golo Kronenberg, Ulrich Dirnagl, Matthias Endres, Christoph Harms
UNLABELLED: The aim of this study was to explore the signaling and neuroprotective effect of transactivator of transcription (TAT) protein transduction of the apoptosis repressor with CARD (ARC) in in vitro and in vivo models of cerebral ischemia in mice. In mice, transient focal cerebral ischemia reduced endogenous ARC protein in neurons in the ischemic striatum at early reperfusion time points, and in primary neuronal cultures, RNA interference resulted in greater neuronal susceptibility to oxygen glucose deprivation (OGD)...
August 3, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Siraj M El Jamal, Erin B Taylor, Zakaria Y Abd Elmageed, Abdulhadi A Alamodi, Denis Selimovic, Abdulaziz Alkhateeb, Matthias Hannig, Sofie Y Hassan, Simeon Santourlidis, Paul L Friedlander, Youssef Haikel, Srinivasan Vijaykumar, Emad Kandil, Mohamed Hassan
BACKGROUND: Tumor response to immunotherapy is the consequence of a concerted crosstalk between cytokines and effector cells. Interferon gamma (IFNγ) is one of the common cytokines coordinating tumor immune response and the associated biological consequences. Although the role of IFNγ in the modulation of tumor immunity has been widely documented, the mechanisms regulating IFNγ-induced cell death, during the course of immune therapy, is not described in detail. RESULTS: IFNγ triggered apoptosis of CLS-354 and RPMI 2650 cells, enhanced the protein expression and activation of indoleamine 2,3-dioxygenase (IDO), and suppressed the basal expression of heme oxygenase-1(HO-1)...
2016: Cell Division
Ruifeng Yao, Zhenhua Ming, Liming Yan, Suhua Li, Fei Wang, Sui Ma, Caiting Yu, Mai Yang, Li Chen, Linhai Chen, Yuwen Li, Chun Yan, Di Miao, Zhongyuan Sun, Jianbin Yan, Yuna Sun, Lei Wang, Jinfang Chu, Shilong Fan, Wei He, Haiteng Deng, Fajun Nan, Jiayang Li, Zihe Rao, Zhiyong Lou, Daoxin Xie
Classical hormone receptors reversibly and non-covalently bind active hormone molecules, which are generated by biosynthetic enzymes, to trigger signal transduction. The α/β hydrolase DWARF14 (D14), which hydrolyses the plant branching hormone strigolactone and interacts with the F-box protein D3/MAX2, is probably involved in strigolactone detection. However, the active form of strigolactone has yet to be identified and it is unclear which protein directly binds the active form of strigolactone, and in which manner, to act as the genuine strigolactone receptor...
August 25, 2016: Nature
Juhyun Song, Mira Jun, Mok-Ryeon Ahn, Oh Yoen Kim
BACKGROUND/OBJECTIVES: Resveratrol, a natural polyphenol, has multiple functions in cellular responses including apoptosis, survival, and differentiation. It also participates in the regulation of inflammatory response and oxidative stress. MicroRNA-Let-7A (miR-Let7A), known as a tumor suppressor miRNA, was recently reported to play a crucial role in both inflammation and apoptosis. Therefore, we examined involvement of miR-Let7A in the modulation of inflammation and cell survival/apoptosis regulated by resveratrol...
August 2016: Nutrition Research and Practice
Jiexia Zhang, Ying Liang, Yongbin Lin, Yuanbin Liu, YouYou, Weiqiang Yin
BACKGROUND: CSTMP, a Tetramethylpyrazine (TMP) analogue, is designed and synthesized based on the pharmacophores of TMP and resveratrol. Recent studies showed that CSTMP had strong protective effects in endothelial cells apoptosis by its anti-oxidant activity. However, the pharmacological function of CSTMP in cancer have not been elucidated to date. The objective of this study was to investigate the anti-cancer effect of CSTMP against human non-small cell lung cancer (NSCLC) A549 cells and the underlying mechanisms...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bing Pei, Miaomiao Yang, Xiaoyan Qi, Xin Shen, Xing Chen, Fayong Zhang
Cerebral ischemia/reperfusion (I/R) is a major cause of severe disability and death all worldwide. However, therapeutic options to minimize the detrimental effects of cerebral I/R injury are limited. Recent research has demonstrated that quercetin mediates neuroprotective effects associated with the activation of the Akt signaling pathway in the cerebral I/R brain. Therefore, the aim of this study was to further investigate the mechanisms of cognitive deficits induced by cerebral I/R injury and the effects of quercetin on these mechanisms...
September 9, 2016: Biochemical and Biophysical Research Communications
Xiaoyu Zhang, Shuchen Li, Mingrong Li, Haiying Huang, Jingyuan Li, Changwei Zhou
Hypoxia-inducible factor-1α (HIF-1α) and toll-like receptor 4 (TLR4) are involved in numerous mechanisms of cancer biology, including cell proliferation and survival; however the interaction of the two factors under hypoxic conditions remains unclear. The present study investigated the in vitro mechanism that results in the suppression of tumor cell growth and cellular functions when HIF-1α is silenced. In the present study, the human hepatocellular carcinoma HepG2 cell line was transfected with short hairpin RNA (shRNA) against HIF-1α and cultured under hypoxic conditions (1% O2 for 24 h)...
August 2016: Oncology Letters
Kaikai Shen, Fangfang Lu, Jianling Xie, Minfeng Wu, Bo Cai, Yurong Liu, Hong Zhang, Hongsheng Tan, Yingyi Pan, Hongxi Xu
Cambogin, a bioactive polycyclic polyprenylated acylphoroglucinol (PPAP) derived from the Garcinia genus, possesses proapoptotic effect in medulloblastoma and breast cancer cells. We have previously demonstrated that the proapoptotic effect of cambogin is driven by the production of reactive oxygen species (ROS). Here we have shown that the inhibitory effect of cambogin on cell proliferation is associated with the loss of mitochondrial transmembrane potential (ΔΨm) and mitochondrial fragmentation. Cambogin also promotes the mutual complex formation of the membrane-bound subunit p22phox of NADPH oxidase 1 (NOX1), as well as the phosphorylation of the cytosolic subunit p47phox, subsequently enhancing membrane-bound NOX1 activity, which leads to increases in intracellular and mitochondrial levels of O2...
July 13, 2016: Oncotarget
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"