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https://www.readbyqxmd.com/read/28641961/wnt5a-and-its-signaling-pathway-in-angiogenesis
#1
REVIEW
Ya-Ning Shi, Neng Zhu, Chan Liu, Hong-Tao Wu, Yu Gui, Duan-Fang Liao, Li Qin
Wnt5a, a secreted glycoprotein, belongs to the noncanonical Wnt family involved in a wide range of organism development and tissue homeostasis. Wnt5a and its signaling pathway can regulate fundamental cellular processes, including specification of cell fate, proliferation, and survival. Accumulating evidence indicates that Wnt5a exhibits dual effects on angiogenesis. The formation of new blood vessels derives from pre-existing vessels via canonical and non-canonical Wnt pathways, depending on cell types, receptors, downstream effectors, and microenvironment...
June 19, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28634819/establishment-of-a-tagged-variant-of-lgr4-receptor-suitable-for-functional-and-expression-studies-in-the-mouse
#2
Vitezslav Kriz, Michaela Krausova, Petra Buresova, Jan Dobes, Dusan Hrckulak, Olga Babosova, Jiri Svec, Vladimir Korinek
Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4) is produced in a broad spectrum of mouse embryonic and adult tissues and its deficiency results in embryonal or perinatal lethality. The LGR4 function was mainly related to its potentiation of canonical Wnt signaling; however, several recent studies associate LGR4 with additional signaling pathways. To obtain a suitable tool for studying the signaling properties of Lgr4, we generated a tagged variant of the Lgr4 receptor using gene targeting in the mouse oocyte...
June 20, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28628652/microrna-433-3p-promotes-osteoblast-differentiation-through-targeting-dkk1-expression
#3
Xiaolin Tang, Jiantao Lin, Guanhai Wang, Jianlin Lu
Dickkopf-1 (DKK1) is a powerful antagonist of canonical WNT signaling pathway, and is regarded as a biomarker for osteoporosis. Its expression is highly correlated with bone mass and osteoblasts maturation. In this study, mouse primary bone marrow cells and osteoblast cell lines were used. Luciferase reporter assay and western blotting methods were employed to validate if miRNA-433-3p epigenetically regulated DKK1 translation. Rat bone marrow derived osteoblasts were infected with lentivirus vector in which miR-433-3p was constructed...
2017: PloS One
https://www.readbyqxmd.com/read/28627829/mir-29a-and-messenger-rna-expression-of-bone-turnover-markers-in-canonical-wnt-pathway-in-patients-with-ankylosing-spondylitis
#4
Jinxian Huang, Guoxiang Song, Zhihua Yin, Zhongchao Fu, Zhizhong Ye
BACKGROUND: Recent studies showed that the canonical Wnt pathway and miR-29a play important roles in the pathogenesis of bone formation. We studied the levels of miR-29a and messenger RNA (mRNA) of bone turnover markers in the canonical Wnt pathway in ankylosing spondylitis (AS). METHODS: The levels of miR-29a and mRNA of bone turnover markers in canonical Wnt pathway from peripheral blood mononuclear cells were determined by real-time quantitative polymerase chain reaction in 38 patients with AS and 32 healthy controls...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28627706/the-wnt-inhibitor-lgk-974-enhances-radiosensitivity-of-hepg2-cells-by-modulating-nrf2-signaling
#5
Dan Tian, Ying Shi, Dexi Chen, Qiang Liu, Feiyue Fan
Nuclear factor (erythroid-derived 2)-like 2 (NRF2) is a master regulator of antioxidant and detoxification activities that can eliminate reactive oxygen species (ROS) produced via irradiation. However, Nrf2 overexpression in liver cancer cells may cause both radioresistance and chemoresistance. Reducing Nrf2 levels can enhance the radiosensitivity of HepG2 cells. Wingless/int-3A (Wnt3A) is a Wnt family protein that mainly activates the canonical Wnt signaling pathway. Recent studies showed that the Axin1-GSK-3β protein complex, a component of the canonical Wnt signaling pathway, can capture Nrf2 and facilitate its ubiquitination and proteasomal degradation in the cytoplasm...
June 14, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28627665/n-acetylcysteine-ameliorates-repetitive-stereotypic-behavior-due-to-its-antioxidant-properties-without-activation-of-the-canonical-wnt-pathway-in-a-valproic-acid-induced-rat-model-of-autism
#6
Yinghua Zhang, Weigang Cui, Qianqian Zhai, Tianran Zhang, Xiaojun Wen
N-acetylcysteine (NAC) is widely used as an antioxidant, and previous studies have suggested that it may have potential as an alternative therapeutic strategy for the treatment of patients with autism. However, the exact effects of NAC administration on the development of autism, as well as the molecular mechanisms underlying its actions, have yet to be fully elucidated. The present study aimed to investigate the effects of NAC on the oxidative status of rats in a valproic acid (VPA)‑induced model of autism, and to examine the involvement of the canonical Wnt signaling pathway in the actions of NAC...
June 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627630/gene-expression-profiling-of-human-blastocysts-from-in%C3%A2-vivo-and-rescue-ivm-with-or-without-melatonin-treatment
#7
Yan Hao, Zhiguo Zhang, Dan Han, Yunxia Cao, Ping Zhou, Zhaolian Wei, Mingrong Lv, Dawei Chen
To evaluate the effect of melatonin supplementation in maturation medium for human 'rescue IVM' and investigate differences in transcriptomic profile of blastocysts developed from oocytes matured in vitro with/without melatonin treatment and in vivo, a total of 314 GV oocytes and 320 MI oocytes were collected from 200 patients younger than 35 years old undergoing ICSI cycle. The oocytes were randomly distributed in the control group (no melatonin) and four other groups of varying melatonin concentrations (10‑11, 10‑9, 10‑7, 10‑5 mol/l)...
June 9, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28626941/binding-of-canonical-wnt-ligands-to-their-receptor-complexes-occurs-in-ordered-plasma-membrane-environments
#8
Erdinc Sezgin, Yagmur Azbazdar, Xue Wen Ng, Cathleen Teh, Kai Simons, Gilbert Weidinger, Thorsten Wohland, Christian Eggeling, Gunes Ozhan
While the cytosolic events of Wnt/β-catenin signaling (canonical Wnt signaling) pathway have been widely studied, only little is known about the molecular mechanisms involved in Wnt binding to its receptors at the plasma membrane. Here, we reveal the influence of the immediate plasma membrane environment on the canonical Wnt-receptor interaction. While the receptors are distributed both in ordered and disordered environments, Wnt binding to its receptors selectively occurs in more ordered membrane environments which appear to co-internalize with the Wnt-receptor complex...
June 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28625976/a-genome-wide-crispr-screen-identifies-genes-critical-for-resistance-to-flt3-inhibitor-ac220
#9
Panpan Hou, Chao Wu, Yuchen Wang, Rui Qi, Dheeraj Bhavanasi, Zhixiang Zuo, Cedric Dos Santos, Shuliang Chen, Yu Chen, Hong Zheng, Hong Wang, Alexander E Perl, Deyin Guo, Jian Huang
Acute myeloid leukemia (AML) is a malignant hematopoietic disease and the most common type of acute leukemia in adults. The mechanisms underlying drug resistance in AML are poorly understood. Activating mutations in FMS-like tyrosine kinase 3 (FLT3) are the most common molecular abnormality in AML. Quizartinib (AC220) is a potent and selective second-generation inhibitor of FLT3. It is in clinical trials for the treatment of relapsed or refractory FLT3-ITD-positive and -negative AML patients and as maintenance therapy...
June 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/28620832/the-pro-renin-receptor-and-its-interaction-partners
#10
REVIEW
Jörg Peters
The prorenin receptor was originally discovered as a receptor that binds renin and prorenin, thereby inducing pro-fibrotic intracellular signal cascades. These effects are partially mediated in vitro by angiotensin (ANG) and partially independent of ANG. Consequently, inhibitors of the interaction between the prorenin and the (pro)renin receptor (PRR) were designed hoping that they may prevent fibrotic tissue damage, for instance, in the kidney. However, this concept was challenged by the fact that overexpression of the PRR was not harmful at all, whereas depletion of the PRR was lethal or markedly detrimental...
June 15, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28620312/thermodynamics-in-gliomas-interactions-between-the-canonical-wnt-beta-catenin-pathway-and-ppar-gamma
#11
REVIEW
Alexandre Vallée, Yves Lecarpentier, Rémy Guillevin, Jean-Noël Vallée
Gliomas cells are the site of numerous metabolic and thermodynamics abnormalities with an increasing entropy rate which is characteristic of irreversible processes driven by changes in Gibbs energy, heat production, intracellular acidity, membrane potential gradient, and ionic conductance. We focus our review on the opposing interactions observed in glioma between the canonical WNT/beta-catenin pathway and PPAR gamma and their metabolic and thermodynamic implications. In gliomas, WNT/beta-catenin pathway is upregulated while PPAR gamma is downregulated...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28618949/iq-domain-gtpase-activating-protein-1-promotes-the-malignant-phenotype-of-invasive-ductal-breast-carcinoma-via-canonical-wnt-pathway
#12
Huan-Yu Zhao, Yang Han, Jian Wang, Lian-He Yang, Xiao-Ying Zheng, Jiang Du, Guang-Ping Wu, En-Hua Wang
IQ-domain GTPase-activating protein 1 is a scaffolding protein with multidomain which plays a role in modulating dishevelled (Dvl) nuclear translocation in canonical Wnt pathway. However, the biological function and mechanism of IQ-domain GTPase-activating protein 1 in invasive ductal carcinoma (IDC) remain unknown. In this study, we found that IQ-domain GTPase-activating protein 1 expression was elevated in invasive ductal carcinoma, which was positively correlated with tumor grade, lymphatic metastasis, and poor prognosis...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28606794/antifibrotic-actions-of-peroxisome-proliferator-activated-receptor-%C3%AE-ligands-in-corneal-fibroblasts-are-mediated-by-%C3%AE-catenin-regulated-pathways
#13
Kye-Im Jeon, Richard P Phipps, Patricia J Sime, Krystel R Huxlin
Wound healing after corneal injury typically involves fibrosis, with transforming growth factor β1 (TGF-β1) as one of its strongest mediators. A class of small molecules-peroxisome proliferator-activated receptor γ (PPARγ) ligands-exert potent antifibrotic effects in the cornea by blocking phosphorylation of p38 mitogen-activated protein kinase (MAPK). However, why this blocks fibrosis remains unknown. Herein, we show that PPARγ ligands (rosiglitazone, troglitazone, and 15-deoxy-Δ12,14-prostaglandin J2) decrease levels of β-catenin...
June 10, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28605700/a-novel-resveratrol-salinomycin-combination-sensitizes-er-positive-breast-cancer-cells-to-apoptosis
#14
Rajkumar Venkatadri, Anand Krishnan V Iyer, Vivek Kaushik, Neelam Azad
BACKGROUND: Resveratrol is a dietary compound that has been widely reported for its anticancer activities. However, successful extrapolation of its effects to pre-clinical studies is met with limited success due to inadequate bioavailability. We investigated the potential of combination therapy to improve the efficacy of resveratrol in a more physiologically relevant dose range. METHODS: The effect of resveratrol on canonical Wnt signaling was evaluated by Western blotting...
April 7, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28605600/activin-and-bmp4-signaling-converge-on-wnt-activation-during-odontogenesis
#15
H-J E Kwon, S Jia, Y Lan, H Liu, R Jiang
Previous studies show that both activin and Bmp4 act as crucial mesenchymal odontogenic signals during early tooth development. Remarkably, mice lacking activin-βA ( Inhba(-/-)) and mice with neural crest-specific inactivation of Bmp4 ( Bmp4(ncko/ncko)) both exhibit bud-stage developmental arrest of the mandibular molar tooth germs while their maxillary molar tooth germs completed morphogenesis. In this study, we found that, whereas expression of Inhba and Bmp4 in the developing tooth mesenchyme is independent of each other, Bmp4(ncko/ncko)Inhba(-/-) compound mutant mice exhibit early developmental arrest of all tooth germs...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28603941/higher-expression-of-wnt5a-protein-in-oral-squamous-cell-carcinoma-compared-with-dysplasia-and-oral-mucosa-with-a-normal-appearance
#16
Zdenka Prgomet, Tommy Andersson, Pia Lindberg
WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis...
June 12, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/28602974/fzd8-a-target-of-p53-promotes-bone-metastasis-in-prostate-cancer-by-activating-canonical-wnt-%C3%AE-catenin-signaling
#17
Qiji Li, Liping Ye, Zhang Xin, Min Wang, Chuyong Lin, Shuai Huang, Wei Guo, Yingrong Lai, Hong Du, Jun Li, Libing Song, Xinsheng Peng
Prostate cancer (PCa) is the second most frequently diagnosed cancer among men and exhibits a high propensity to metastasize to bone. Currently, bone metastasis remains incurable, and therapies are limited. A better understanding of the molecular mechanisms involved in PCa bone metastasis is needed to develop more effective therapeutics for this disease. Herein, we reported that among the FZD family, FZD8 was robustly upregulated in bone-metastastic PCa cell lines and tissues. High levels of FZD8 expression were significantly positively associated with clinical tumor progression and bone metastasis...
June 7, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28589433/overexpression-of-dishevelled-2-is-involved-in-tumor-metastasis-and-is-associated-with-poor-prognosis-in-hepatocellular-carcinoma
#18
C Zhang, C Li, X Chen, Y Zhou, B Yin, R Ni, Y Zhang, J Liu
PURPOSE: Although hepatocellular carcinoma (HCC) is one of the most common malignant tumors, its molecular mechanism is still unknown. Dishevelled 2 (Dvl2) is one of the downstream targets of non-canonical Wnt signaling, which has been demonstrated to be of great importance in the progression of cancers. Nevertheless, the expression mechanisms and physiological significance of Dvl2 in HCC remain unclear. METHODS: Western blotting and immunohistochemistry were used to measure Dvl2 protein expression in HCC and adjacent normal tissues of 101 patients...
June 6, 2017: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/28588489/fh535-suppresses-osteosarcoma-growth-in-vitro-and-inhibits-wnt-signaling-through-tankyrases
#19
Carl T Gustafson, Tewodros Mamo, Kristen L Shogren, Avudaiappan Maran, Michael J Yaszemski
Osteosarcoma (OS) is an aggressive primary bone tumor which exhibits aberrantly activated Wnt signaling. The canonical Wnt signaling cascade has been shown to drive cancer progression and metastasis through the activation of β-catenin. Hence, small molecule inhibitors of Wnt targets are being explored as primary or adjuvant chemotherapy. In this study, we have investigated the ability of FH535, an antagonist of Wnt signaling, to inhibit the growth of OS cells. We found that FH535 was cytotoxic in all OS cell lines which were tested (143b, U2OS, SaOS-2, HOS, K7M2) but well tolerated by normal human osteoblast cells...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28587781/wnt5a-induces-catabolic-signaling-and-matrix-metalloproteinase-production-in-human-articular-chondrocytes
#20
G Huang, S Chubinskaya, W Liao, R F Loeser
OBJECTIVE: Aberrant Wnt signaling may contribute to osteoarthritis (OA) but the Wnt family members involved have not been fully identified. The purpose of this study was to investigate the role of Wnt5a as a potential mediator of cartilage destruction in OA. DESIGN: Immunohistochemistry to detect Wnt5a was performed using normal and OA human articular cartilage. Cultured normal human chondrocytes were treated with fibronectin fragments (FN-f) as a catabolic stimulus or recombinant Wnt5a protein with or without pretreatment using a panel of signaling inhibitors...
June 3, 2017: Osteoarthritis and Cartilage
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