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Non muscle myosin IIA

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https://www.readbyqxmd.com/read/28711870/symmetrical-retrograde-actin-flow-in-the-actin-fusion-structure-is-involved-in-osteoclast-fusion
#1
Jiro Takito, Hirotada Otsuka, Satoshi Inoue, Tsubasa Kawashima, Masanori Nakamura
The aim of this study was to elucidate the role of the zipper-like structure (ZLS), a podosome-related structure that transiently appears at the cell contact zone, in osteoclast fusion. Live-cell imaging of osteoclasts derived from RAW264.7 cells transfected with EGFP-actin revealed consistent symmetrical retrograde actin flow in the ZLS, but not in the podosome cluster, the podosome ring or the podosome belt. Confocal imaging showed that the distributions of F-actin, vinculin, paxillin and zyxin in the ZLS were different from those in the podosome belt...
July 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28606474/regulation-of-intracellular-trafficking-and-secretion-of-adiponectin-by-myosin-ii
#2
Deepa Bedi, John C Dennis, Edward E Morrison, Tim D Braden, Robert L Judd
Adiponectin is a protein secreted by white adipocytes that plays an important role in insulin action, energy homeostasis and the development of atherosclerosis. The intracellular localization and trafficking of GLUT4 and leptin in adipocytes has been well studied, but little is known regarding the intracellular trafficking of adiponectin. Recent studies have demonstrated that constitutive adiponectin secretion is dependent on PIP2 levels and the integrity of cortical F-actin. Non-muscle myosin II is an actin-based motor that is associated with membrane vesicles and participates in vesicular trafficking in mammalian cells...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28539835/the-myosin-ii-inhibitor-blebbistatin-ameliorates-fecl3-induced-arterial-thrombosis-via-the-gsk3%C3%AE-nf-%C3%AE%C2%BAb-pathway
#3
Yuanyuan Zhang, Long Li, Yazheng Zhao, Han Han, Yang Hu, Di Liang, Boyang Yu, Junping Kou
Arterial thrombosis and its related diseases are major healthcare problems worldwide. Blebbistatin is an inhibitor of myosin II, which plays an important role in thrombosis. The aim of our study is to explore the effect and potential mechanism of blebbistatin on arterial thrombosis. A ferric chloride (FeCl3) solution at a concentration of 5% was used to induce carotid artery thrombosis in mice. Immunohistochemistry and immunofluorescence staining were used to detect the expression or activation of non-muscle myosin heavy chain IIA (NMMHC IIA), tissue factor (TF), GSK3β and NF-κB...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28526784/trpv4-mediates-the-ca-2-influx-required-for-the-interaction-between-flightless-1-and-non-muscle-myosin-and-collagen-remodeling
#4
Pamma D Arora, Madeleine Di Gregorio, Pei He, Christopher A McCulloch
Fibroblasts remodel extracellular matrix collagen, in part, through phagocytosis. This process requires formation of cell extensions, which in turn involves interaction of the actin-binding protein flightless-1 (FliI) with non-muscle myosin IIA (NMMIIA; heavy chain encoded by MYH9) at cell-matrix adhesion sites. As Ca(2+) plays a central role in controlling actomyosin-dependent functions, we examined how Ca(2+) controls the generation of cell extensions and collagen remodeling. Ratio fluorimetry demonstrated localized Ca(2+) influx at the extensions of fibroblasts...
July 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28361956/role-for-formin-like-1-dependent-acto-myosin-assembly-in-lipid-droplet-dynamics-and-lipid-storage
#5
Simon G Pfisterer, Gergana Gateva, Peter Horvath, Juho Pirhonen, Veijo T Salo, Leena Karhinen, Markku Varjosalo, Samppa J Ryhänen, Pekka Lappalainen, Elina Ikonen
Lipid droplets (LDs) are cellular organelles specialized in triacylglycerol (TG) storage undergoing homotypic clustering and fusion. In non-adipocytic cells with numerous LDs this is balanced by poorly understood droplet dissociation mechanisms. We identify non-muscle myosin IIa (NMIIa/MYH-9) and formin-like 1 (FMNL1) in the LD proteome. NMIIa and actin filaments concentrate around LDs, and form transient foci between dissociating LDs. NMIIa depletion results in decreased LD dissociations, enlarged LDs, decreased hydrolysis and increased storage of TGs...
March 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/28345668/c9orf135-encodes-a-membrane-protein-whose-expression-is-related-to-pluripotency-in-human-embryonic-stem-cells
#6
Shixin Zhou, Yinan Liu, Yumin Ma, Xiaoyan Zhang, Yang Li, Jinhua Wen
Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency factors OCT4, SOX2, and NANOG. Human ESCs express two C9ORF135 isoforms, the longer of which encodes a membrane-associated protein, as determined by immunostaining and western blotting of fractionated cell lysates...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303576/non-muscle-nm-myosin-heavy-chain-phosphorylation-regulates-the-formation-of-nm-myosin-filaments-adhesome-assembly-and-smooth-muscle-contraction
#7
Wenwu Zhang, Susan J Gunst
KEY POINTS: Non-muscle (NM) and smooth muscle (SM) myosin II are both expressed in smooth muscle tissues, however the role of NM myosin in SM contraction is unknown. Contractile stimulation of tracheal smooth muscle tissues stimulates phosphorylation of the NM myosin heavy chain on Ser1943 and causes NM myosin filament assembly at the SM cell cortex. Expression of a non-phosphorylatable NM myosin mutant, NM myosin S1943A, in SM tissues inhibits ACh-induced NM myosin filament assembly and SM contraction, and also inhibits the assembly of membrane adhesome complexes during contractile stimulation...
July 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28216317/tropomyosin-isoforms-specify-functionally-distinct-actin-filament-populations-in%C3%A2-vitro
#8
Gergana Gateva, Elena Kremneva, Theresia Reindl, Tommi Kotila, Konstantin Kogan, Laurène Gressin, Peter W Gunning, Dietmar J Manstein, Alphée Michelot, Pekka Lappalainen
Actin filaments assemble into a variety of networks to provide force for diverse cellular processes [1]. Tropomyosins are coiled-coil dimers that form head-to-tail polymers along actin filaments and regulate interactions of other proteins, including actin-depolymerizing factor (ADF)/cofilins and myosins, with actin [2-5]. In mammals, >40 tropomyosin isoforms can be generated through alternative splicing from four tropomyosin genes. Different isoforms display non-redundant functions and partially non-overlapping localization patterns, for example within the stress fiber network [6, 7]...
March 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28199848/discoidin-domain-receptor-1-mediates-myosin-dependent-collagen-contraction
#9
Nuno M Coelho, Pamma D Arora, Sander van Putten, Stellar Boo, Petar Petrovic, Alyna Xue Lin, Boris Hinz, Christopher A McCulloch
Discoidin domain receptor 1 (DDR1) is a tyrosine kinase collagen adhesion receptor that mediates cell migration through association with non-muscle myosin IIA (NMIIA). Because DDR1 is implicated in cancer fibrosis, we hypothesized that DDR1 interacts with NMIIA to enable collagen compaction by traction forces. Mechanical splinting of rat dermal wounds increased DDR1 expression and collagen alignment. In periodontal ligament of DDR1 knockout mice, collagen mechanical reorganization was reduced >30%. Similarly, cultured cells with DDR1 knockdown or expressing kinase-deficient DDR1d showed 50% reduction of aligned collagen...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28160562/the-tumor-suppressor-capability-of-p53-is-dependent-on-non-muscle-myosin-iia-function-in-head-and-neck-cancer
#10
Sonya D Coaxum, Jessica Tiedeken, Elizabeth Garrett-Mayer, Jeffrey Myers, Steven A Rosenzweig, David M Neskey
Over 300,000 patients develop squamous cell carcinoma of the head and neck (HNSCC) worldwide with 25-30% of patients ultimately dying from their disease. Currently, molecular biomarkers are not used in HNSCC but several genes have been identified including mutant TP53 (mutp53). Our recent work has identified an approach to stratify patients with tumors harboring high or low risk TP53 mutations. Non-muscle Myosin IIA (NMIIA) was recently identified as a tumor suppressor in HNSCC. We now demonstrate that low MYH9 expression is associated with decreased survival in patients with head and neck cancer harboring low-risk mutp53 but not high-risk mutp53...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28039206/endoplasmic-reticulum-chaperone-gp96-controls-actomyosin-dynamics-and-protects-against-pore-forming-toxins
#11
Francisco Sarmento Mesquita, Cláudia Brito, Maria J Mazon Moya, Jorge Campos Pinheiro, Serge Mostowy, Didier Cabanes, Sandra Sousa
During infection, plasma membrane (PM) blebs protect host cells against bacterial pore-forming toxins (PFTs), but were also proposed to promote pathogen dissemination. However, the details and impact of blebbing regulation during infection remained unclear. Here, we identify the endoplasmic reticulum chaperone Gp96 as a novel regulator of PFT-induced blebbing. Gp96 interacts with non-muscle myosin heavy chain IIA (NMHCIIA) and controls its activity and remodelling, which is required for appropriate coordination of bleb formation and retraction...
February 2017: EMBO Reports
https://www.readbyqxmd.com/read/27693494/bradykinin-promotes-migration-and-invasion-of-hepatocellular-carcinoma-cells-through-trpm7-and-mmp2
#12
Yijie Chen, Yang Yu, Shuya Sun, Zhaotong Wang, Peiqing Liu, Shenglan Liu, Jianmin Jiang
Tumor metastasis is the main reason of death for hepatocellular carcinoma (HCC) patients. Cell migration and invasion are two prerequisites for tumor metastasis, in which TRPM7 and MMPs play an important role. In our study, we found that bradykinin (BK) could upregulate the expression of TRPM7 and dynamically regulate the phosphorylation of non-muscle myosin IIA heavy chain (NMHC-IIA) Ser-1943 in HepG2 cells. The influx of Ca(2+) via TRPM7 was necessary for elevating the activity of m-calpain and μ-calpain...
November 15, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27669196/non-invasive-assessment-of-skeletal-muscle-myosin-heavy-chain-expression-in-trained-and-untrained-men
#13
Andrew C Fry, Terry J Housh, Joel B Cramer, Joseph P Weir, Travis W Beck, Brian K Schilling, Jonathan D Miller, Justin X Nicoll
Numerous conditions and types of physical activity (e.g., exercise, aging, muscle-related diseases) can influence muscle fiber types and the proteins expressed. To date, muscle fibers can only be characterized by actually obtaining a tissue sample using the invasive muscle biopsy procedure. Mechanomyography (MMG) is the assessment of the vibration properties of contracting skeletal muscle, and has been proposed as a possible non-invasive method for muscle fiber analysis. Therefore, the purpose of this project was to examine the feasibility of using MMG and muscle performance measures to non-invasively assess muscle fiber characteristics...
September 20, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27634189/nmmhc-iia-dependent-nuclear-location-of-cxcr4-promotes-migration-and-invasion-in-renal-cell-carcinoma
#14
Zhipeng Xu, Peng Li, Dan Wei, Zhixiang Wang, Yi Bao, Jipeng Sun, Le Qu, Linhui Wang
The chemokine receptor cysteine (C)-X-C receptor (CXCR4) is a G-protein-coupled receptor that exerts a vital role in distant metastasis of renal cell carcinoma (RCC). Emerging evidence demonstrates that CXCR4 as the cytomembrane receptor translocated into the nucleus to facilitate cell migration and, therefore, determine the prognosis of several types of malignancies. However, the biological mechanism of nuclear location of CXCR4 remains unclear. In the present study, we confirmed the significant implications of the putative nuclear localization sequence (NLS) '146RPRK149̓ on CXCR4 subcellular localization and metastatic potential by point-mutation assay in RCC cell lines...
November 2016: Oncology Reports
https://www.readbyqxmd.com/read/27535804/the-role-of-rab6a-and-phosphorylation-of-non-muscle-myosin-iia-tailpiece-in-alcohol-induced-golgi-disorganization
#15
Armen Petrosyan, Carol A Casey, Pi-Wan Cheng
Abnormalities in the Golgi apparatus function are important to the development of alcoholic liver injury. We recently reported that Golgi disorganization in ethanol (EtOH)-treated hepatocytes is caused by impaired dimerization of the largest Golgi matrix protein, giantin. However, little is known about the mechanism which forces fragmentation. Here, in both HepG2 cells overexpressing alcohol dehydrogenase and in rat hepatocytes, we found that EtOH administration reduces the complex between giantin and Rab6a GTPase and results in the S1943 phosphorylation of non-muscle Myosin IIA (NMIIA) heavy chain, thus facilitating NMIIA association with Golgi enzymes, as detected by biochemical approaches and 3D Structured Illumination Microscopy...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27478065/appl1-promotes-glucose-uptake-in-response-to-mechanical-stretch-via-the-pkc%C3%AE-non-muscle-myosin-iia-pathway-in-c2c12-myotubes
#16
Tsugumichi Saito, Shuichi Okada, Yoko Shimoda, Yuko Tagaya, Aya Osaki, Eijiro Yamada, Ryo Shibusawa, Yasuyo Nakajima, Atsushi Ozawa, Tetsurou Satoh, Masatomo Mori, Masanobu Yamada
Expression of adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper containing 1 (APPL1) promoted glucose transporter 4 (GLUT4) translocation and glucose uptake in adipose and muscle tissues in response to stimulation with insulin, adiponectin, or exercise. In response to mechanical stretch, knockdown of APPL1 in C2C12 myotubes suppressed glucose uptake. APPL1-induced increased glucose uptake was mediated by protein kinase C (PKC) ζ but not AKT, AMPK, or calmodulin-dependent protein kinase...
November 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27418229/multi-level-changes-in-protein-dynamics-upon-complex-formation-of-the-calcium-loaded-s100a4-with-a-non-muscle-myosin-iia-tail-fragment
#17
Gyula Pálfy, Bence Kiss, László Nyitray, Andrea Bodor
Dysregulation in Ca2+-binding S100 proteins playsimportant role in various diseases. The asymmetric complex of Ca2+-loaded S100A4 with non-muscle myosin IIA has highstability and highly increased Ca2+-affinity. Here weinvestigated the possible causes of this allosteric effect by NMR spectroscopic approaches. Chemical shift basedsecondary structure analysis did not show substantial changes for the complex. Backbone dynamics revealed slow time-scale local motions in the H1 helices of the homodimeric S100A4,which diminished in the complex and could be accompanied by an increase in dimer stability...
July 15, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27398909/mechanical-regulation-of-transcription-controls-polycomb-mediated-gene-silencing-during-lineage%C3%A2-commitment
#18
Huy Quang Le, Sushmita Ghatak, Ching-Yan Chloé Yeung, Frederik Tellkamp, Christian Günschmann, Christoph Dieterich, Assa Yeroslaviz, Bianca Habermann, Ana Pombo, Carien M Niessen, Sara A Wickström
Tissue mechanics drive morphogenesis, but how forces are sensed and transmitted to control stem cell fate and self-organization remains unclear. We show that a mechanosensory complex of emerin (Emd), non-muscle myosin IIA (NMIIA) and actin controls gene silencing and chromatin compaction, thereby regulating lineage commitment. Force-driven enrichment of Emd at the outer nuclear membrane of epidermal stem cells leads to defective heterochromatin anchoring to the nuclear lamina and a switch from H3K9me2,3 to H3K27me3 occupancy at constitutive heterochromatin...
August 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27393652/genetic-association-of-myh-genes-with-hereditary-hearing-loss-in-korea
#19
Sang-Joo Kim, Seokwon Lee, Hong-Joon Park, Tae-Hun Kang, Borum Sagong, Jeong-In Baek, Se-Kyung Oh, Jae Young Choi, Kyu-Yup Lee, Un-Kyung Kim
BACKGROUND: Myosin is a key protein involved in regulating the shape and motility of cells. The MYH9 and MYH14 genes, which encode non-muscle myosin heavy chain IIA (NMMHC II-A) and IIC (NMMHC II-C), respectively, are expressed in the inner ear. These myosin genes are known to be associated with autosomal dominant non-syndromic hearing loss (ADNSHL); however, genetic studies in patients with ADNSHL in Korea have rarely been reported. METHODS: We analyzed the MYH9 and MYH14 genes in 75 Korean patients with ADNSHL...
October 10, 2016: Gene
https://www.readbyqxmd.com/read/27374701/dt-13-attenuates-human-lung-cancer-metastasis-via-regulating-nmiia-activity-under-hypoxia-condition
#20
Xiao-Hui Wei, Sen-Sen Lin, Yang Liu, Ren-Ping Zhao, Ghulam Jilany Khan, Hong-Zhi Du, Ting-Ting Mao, Bo-Yang Yu, Rui-Ming Li, Sheng-Tao Yuan, Li Sun
Cancer metastasis plays a major role in tumor deterioration. Metastatic processes are known to be regulated by hypoxic microenvironment and non-muscle myosin IIA (NMIIA). DT-13, a bioactive saponin monomer isolated from Ophiopogon japonicus, has been reported to inhibit various cancer metastasis, but whether NMIIA is involved in the anti-metastatic activity of DT-13 under hypoxia remains to be determined. Thus, this study aims to clarify the role of DT-13 in regulating 95D cell metastasis under hypoxic microenvironment and to further investigate whether NMIIA is involved in the anti-metastatic mechanism of DT-13...
August 2016: Oncology Reports
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