keyword
MENU ▼
Read by QxMD icon Read
search

Bone marrow engraftment

keyword
https://www.readbyqxmd.com/read/28817977/a-short-and-efficient-transduction-protocol-of-mouse-hematopoietic-stem-cells-with-lentiviral-vectors
#1
Maria Fernandez-Garcia, Cristina Mesa-Nuñez, Elena Almarza, Juan Bueren, Rosa Yañez
Transduction of hematopoietic stem and progenitor cells (HSPC) with therapeutic lentiviral vectors (LVs) constitutes a new therapeutic option for the treatment of different monogenic diseases affecting the lympho-hematopoietic system. The development of detailed preclinical studies of gene therapy in animal disease models constitutes an essential step in expanding the application of gene therapy in a wide variety of inherited and acquired diseases. Here we describe an efficient protocol to transduce HSPCs from wild-type and Fanconi anemia mice with either gene marking or therapeutic LVs...
August 17, 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/28816238/acute-myeloid-leukemia-transforms-the-bone-marrow-niche-into-a-leukemia-permissive-microenvironment-through-exosome-secretion
#2
B Kumar, M Garcia, L Weng, X Jung, J L Murakami, X Hu, T McDonald, A Lin, A R Kumar, D L DiGiusto, A S Stein, V A Pullarkat, S K Hui, N Carlesso, Y-H Kuo, R Bhatia, G Marcucci, C-C Chen
Little is known about how leukemia cells alter the bone marrow (BM) niche to facilitate their own growth and evade chemotherapy. Here, we provide evidence that acute myeloid leukemia (AML) blasts remodel the BM niche into a leukemia-growth-permissive and normal-hematopoiesis-suppressive microenvironment through exosome secretion. Either engrafted AML cells or AML-derived exosomes increased mesenchymal stromal progenitors and blocked osteolineage development and bone formation in vivo. Pre-conditioning with AML-derived exosomes 'primed' the animals for accelerated AML growth...
August 17, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28809828/bioengineering-of-humanized-bone-marrow-microenvironments-in-mouse-and-their-visualization-by-live-imaging
#3
Diana Passaro, Ander Abarrategi, Katie Foster, Linda Ariza-McNaughton, Dominique Bonnet
Human hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, an intricate, multifactorial network of components producing cytokines, growth factors, and extracellular matrix. The ability of HSCs to remain quiescent, self-renew or differentiate, and acquire mutations and become malignant depends upon the complex interactions they establish with different stromal components. To observe the crosstalk between human HSCs and the human BM niche in physiological and pathological conditions, we designed a protocol to ectopically model and image a humanized BM niche in immunodeficient mice...
August 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28800741/erythrocyte-depletion-from-bone-marrow-performance-evaluation-after-50-clinical-scale-depletions-with-spectra-optia-bmc
#4
Soo-Zin Kim-Wanner, Gesine Bug, Juliane Steinmann, Salem Ajib, Nadine Sorg, Carolin Poppe, Milica Bunos, Eva Wingenfeld, Christiane Hümmer, Beate Luxembourg, Erhard Seifried, Halvard Bonig
BACKGROUND: Red blood cell (RBC) depletion is a standard graft manipulation technique for ABO-incompatible bone marrow (BM) transplants. The BM processing module for Spectra Optia, "BMC", was previously introduced. We here report the largest series to date of routine quality data after performing 50 clinical-scale RBC-depletions. METHODS: Fifty successive RBC-depletions from autologous (n = 5) and allogeneic (n = 45) BM transplants were performed with the Spectra Optia BMC apheresis suite...
August 11, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28798321/crispr-cas9-mediated-deletion-of-foxn1-in-nod-scid-il2rg-mice-results-in-severe-immunodeficiency
#5
Xinru Wei, Yunxin Lai, Baiheng Li, Le Qin, Youdi Xu, Simiao Lin, Suna Wang, Qiting Wu, Qiubin Liang, Guohua Huang, Qiuhua Deng, Pentao Liu, Donghai Wu, Liangxue Lai, Yao Yao, Peng Li
Immunodeficient mice engrafted with either normal or cancerous human cells are widely used in basic and translational research. In particular, NOD/SCID/IL2rg(-/-) mice can support the growth of various types of human cancer cells. However, the hairs of these mice interfere with the observation and imaging of engrafted tissues. Therefore, novel hairless strains exhibiting comparable immunodeficiency would be beneficial. Recently, the CRISPR/Cas9 system has been used for efficient multiplexed genome editing. In the present study, we generated a novel strain of nude NOD/SCID/IL2rg(-/-) (NSIN) mice by knocking out Foxn1 from NOD/SCID/IL2rg(-/-) (NSI) mice using the CRISPR/Cas9 system...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28782281/systemic-administration-of-bone-marrow-derived-cells-leads-to-better-uterine-engraftment-than-use-of-uterine-derived-cells-or-local-injection
#6
Ying Liu, Reshef Tal, Nicola Pluchino, Ramanaiah Mamillapalli, Hugh S Taylor
Stem cells are recruited to the uterus where they differentiate into endometrial cells and have been suggested as potential therapy for uterine injury such as Asherman's syndrome. However, it is unknown whether local intrauterine injection may result in better stem cell engraftment of the uterus compared with systemic administration, and whether uterine-derived cells (UDCs) may confer an advantage over BM-derived cells (BMDCs). Mice underwent local injury to a single uterine horn. Green fluorescent protein (GFP)-expressing BMDCs, UDCs or saline (control) were injected either intravenously or locally (uterine lumen) into wild-type recipients...
August 7, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28777945/long-term-engraftment-of-primary-bone-marrow-stromal-cells-repairs-niche-damage-and-improves-hematopoietic-stem-cell-transplantation
#7
Jean-Paul Abbuehl, Zuzana Tatarova, Werner Held, Joerg Huelsken
Hematopoietic stem cell (HSC) transplantation represents a curative treatment for various hematological disorders. However, delayed reconstitution of innate and adaptive immunity often causes fatal complications. HSC maintenance and lineage differentiation are supported by stromal niches, and we now find that bone marrow stroma cells (BMSCs) are severely and permanently damaged by the pre-conditioning irradiation required for efficient HSC transplantation. Using mouse models, we show that stromal insufficiency limits the number of donor-derived HSCs and B lymphopoiesis...
August 3, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28771276/downregulation-of-hypoxia-inducible-factor-1%C3%AE-contributes-to-impaired-megakaryopoiesis-in-immune-thrombocytopenia
#8
Jiaqian Qi, Tao You, Tingting Pan, Qi Wang, Li Zhu, Yue Han
Impaired megakaryocyte maturation and exaggerated platelet destruction play a pivotal role in the pathogenesis of immune thrombocytopenia (ITP). Previous studies have shown that HIF-1α promotes the homing and engraftment of haematopoietic stem cells (HSCs), thereby stimulating HSC differentiation. However, whether HIF-1α plays a role in megakaryocytic maturation and platelet destruction in ITP remains elusive. Using enzyme-linked immunosorbent assays (ELISAs), we demonstrated that there were lower HIF-1α levels in the bone marrow (BM) of ITP patients than in that of healthy donors and patients with chemotherapy-related thrombocytopenia...
August 3, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28770214/cardiac-stem-cells-for-myocardial-regeneration-they-are-not-alone
#9
REVIEW
Yin Yee Leong, Wai Hoe Ng, Georgina M Ellison-Hughes, Jun Jie Tan
Heart failure is the number one killer worldwide with ~50% of patients dying within 5 years of prognosis. The discovery of stem cells, which are capable of repairing the damaged portion of the heart, has created a field of cardiac regenerative medicine, which explores various types of stem cells, either autologous or endogenous, in the hope of finding the "holy grail" stem cell candidate to slow down and reverse the disease progression. However, there are many challenges that need to be overcome in the search of such a cell candidate...
2017: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28769930/anti-interleukin-6-promotes-allogeneic-bone-marrow-engraftment-and-prolonged-graft-survival-in-an-irradiation-free-murine-transplant-model
#10
Nicolas Granofszky, Andreas M Farkas, Moritz Muckenhuber, Benedikt Mahr, Lukas Unger, Svenja Maschke, Nina Pilat, Raimund Holly, Mario Wiletel, Heinz Regele, Thomas Wekerle
Transfer of recipient regulatory T cells (Tregs) induces mixed chimerism and tolerance in an irradiation-free bone marrow (BM) transplantation (BMT) model involving short-course co-stimulation blockade and mTOR inhibition. Boosting endogenous Tregs pharmacologically in vivo would be an attractive alternative avoiding the current limitations of performing adoptive cell therapy in the routine clinical setting. Interleukin-6 (IL-6) potently inhibits Treg differentiation and its blockade was shown to increase Treg numbers in vivo...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28764810/cd106-is-a-novel-mediator-of-bone-marrow-mesenchymal-stem-cells-via-nf-%C3%AE%C2%BAb-in-the-bone-marrow-failure-of-acquired-aplastic-anemia
#11
Shihong Lu, Meili Ge, Yizhou Zheng, Jianping Li, Xiaoming Feng, Sizhou Feng, Jinbo Huang, Ying Feng, Donglin Yang, Jun Shi, Fang Chen, Zhongchao Han
BACKGROUND: Acquired aplastic anemia (AA) is characterized by deficiency or dysfunction of the bone marrow (BM) microenvironment. However, little is known about the impairment of BM-derived mesenchymal stem cells (MSCs) in AA patients. METHODS: We used Illumina HiSeqTM 2000 sequencing, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry (FCM), and Western blotting to test the expression of CD106 gene (vascular cell adhesion molecule 1 (VCAM1)) and CD106 protein of BM-MSCs...
August 1, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28760119/autologous-stem-cell-rescue-for-graft-failure-of-second-allogeneic-stem-cell-transplant-after-engraftment-of-primary-allogeneic-transplant
#12
Atsushi Watanabe, Takeshi Inukai, Koshi Akahane, Shinpei Somazu, Hiroko Oshiro, Kumiko Goi, Keiichi Koizumi, Norikazu Harii, Kenichi Matsuda, Kanji Sugita
Here, we describe a case of primary graft failure with severe sepsis in a boy who experienced frequent relapses of osteosarcoma. The patient had undergone haploidentical bone marrow transplant after engraftment of unrelated cord blood transplant performed 10 months earlier. Considering his severe condition, we transfused autologous peripheral stem cells along with a single dose of etoposide (50 mg/m2). Granulocyte engraftment was confirmed on human leukocyte antigen-microsatellite analysis of bone marrow on day 14...
July 31, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28757877/trophic-activity-and-phenotype-of-adipose-tissue-derived-mesenchymal-stem-cells-as-a-background-of-their-regenerative-potential
#13
REVIEW
Beata Kocan, Aleksandra Maziarz, Jacek Tabarkiewicz, Takahiro Ochiya, Agnieszka Banaś-Ząbczyk
There has been an increased interest in mesenchymal stem cells from adipose tissue, due to their abundance and accessibility with no ethical concerns. Their multipotent properties make them appropriate for regenerative clinical applications. It has been shown that adipose-derived stem cells (ASCs) may differ between the origin sites. Moreover, a variety of internal and external factors may affect their biological characteristics, as what we aimed to highlight in this review. It has been demonstrated that ASCs secrete multiple trophic factors that are capable of stimulating cell proliferation and differentiation and migration of various cell types...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28754835/in-vivo-functional-selection-identifies-cardiotrophin-1-as-a-cardiac-engraftment-factor-for-mesenchymal-stromal-cells
#14
Francesca Bortolotti, Giulia Ruozi, Antonella Falcione, Sara Doimo, Matteo Dal Ferro, Pierluigi Lesizza, Lorena Zentilin, Lawrence Banks, Serena Zacchigna, Mauro Giacca
Background -Transplantation of cells into the infarcted heart has significant potential to improve myocardial recovery; however, low efficacy of cell engraftment still limits therapeutic benefit. Here we describe a method for the unbiased, in vivo selection of cytokines that improve mesenchymal stromal cell (MSC) engraftment into the heart in both normal conditions and after myocardial infarction. Methods -An arrayed library of 80 secreted factors, including most of the currently known interleukins and chemokines, were individually cloned into adeno-associated viral (AAV) vectors...
July 28, 2017: Circulation
https://www.readbyqxmd.com/read/28752258/allogeneic-reduced-intensity-hematopoietic-stem-cell-transplantation-for-chronic-granulomatous-disease-a-single-center-prospective-trial
#15
Mark Parta, Corin Kelly, Nana Kwatemaa, Narda Theobald, Diane Hilligoss, Jing Qin, Douglas B Kuhns, Christa Zerbe, Steven M Holland, Harry Malech, Elizabeth M Kang
PURPOSE: The purpose of this study was to evaluate engraftment and adverse events with a conditioning and prophylactic regimen intended to achieve high rates of engraftment with minimal graft-versus-host disease (GVHD) in allogeneic transplantation for chronic granulomatous disease in a single center. METHODS: Forty patients, 37 male, with chronic granulomatous disease were transplanted. Transplant products were matched sibling peripheral blood stem cells (PBSCs) in four and matched unrelated donor (MUD) bone marrow in three, and one patient received mismatched unrelated PBSCs...
July 28, 2017: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/28747915/t-cell-lymphoma-and-leukemia-in-severe-combined-immunodeficiency-pigs-following-bone-marrow-transplantation-a-case-report
#16
Ellis J Powell, Jared Graham, N M Ellinwood, Jesse Hostetter, Michael Yaeger, Chak-Sum Ho, Lynden Gault, Veronica Norlin, Elizabeth N Snella, Jackie Jens, Emily H Waide, Adeline N Boettcher, Maureen Kerrigan, Raymond R R Rowland, Jason W Ross, Jack C M Dekkers, Christopher K Tuggle
After the discovery of naturally occurring severe combined immunodeficiency (SCID) within a selection line of pigs at Iowa State University, we found two causative mutations in the Artemis gene: haplotype 12 (ART12) and haplotype 16 (ART16). Bone marrow transplants (BMTs) were performed to create genetically SCID and phenotypically immunocompetent breeding animals to establish a SCID colony for further characterization and research utilization. Of nine original BMT transfer recipients, only four achieved successful engraftment...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28739140/the-il-4-receptor-%C3%AE-has-a-critical-role-in-bone-marrow-derived-fibroblast-activation-and-renal-fibrosis
#17
Hua Liang, Zhengmao Zhang, Jingyin Yan, Yuguo Wang, Zhaoyong Hu, William E Mitch, Yanlin Wang
Renal fibrosis is a common pathway leading to the progression of chronic kidney disease, and bone marrow-derived fibroblasts contribute significantly to the development of renal fibrosis. However, the signaling mechanisms underlying the activation of these fibroblasts are not completely understood. Here, we examined the role of IL-4 receptor α (IL-4Rα) in the activation of myeloid fibroblasts in two experimental models of renal fibrosis. Compared with wild-type mice, IL-4Rα knockout mice accumulated fewer bone marrow-derived fibroblasts and myofibroblasts in their kidneys...
July 21, 2017: Kidney International
https://www.readbyqxmd.com/read/28726516/targeting-nsg-mice-engrafting-cells-with-a-clinically-applicable-lentiviral-vector-corrects-osteoclasts-in-infantile-malignant-osteopetrosis
#18
Ilana Moscatelli, Henrik Löfvall, Christian Schneider Thudium, Michael Rothe, Carmen Montano, Zsuzsanna Kertész, Mehtap Sirin, Ansgar Schulz, Axel Schambach, Kim Henriksen, Johan Richter
Infantile malignant osteopetrosis (IMO) is a rare, lethal, autosomal recessive disorder characterized by nonfunctional osteoclasts. More than 50% of the patients have mutations in the TCIRG1 gene, encoding for a subunit of the osteoclast proton pump. The aim of this study was to develop a clinically applicable lentiviral vector expressing TCIRG1 to correct osteoclast function in IMO. We compared two mammalian promoters: elongation factor 1α short promoter (EFS) and chimeric myeloid promoter (ChimP). EFS was chosen for continued experiments as it performed better...
July 20, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28713641/higher-serum-alanine-transaminase-levels-in-male-urokinase-type-plasminogen-activator-transgenic-mice-are-associated-with-improved-engraftment-of-hepatocytes-but-not-liver-sinusoidal-endothelial-cells
#19
Marina E Fomin, Ashley I Beyer, Jean Publicover, Kai Lu, Sonia Bakkour, Graham Simmons, Marcus O Muench
The effects of sex on the degree of liver damage and human cell engraftment were investigated in immunodeficient urokinase-type plasminogen activator-transgenic (uPA-NOG) mice. Liver damage, measured by serum alanine transaminase (ALT) levels, was compared in male and female uPA-NOG mice of different ages. Male mice had significantly higher ALT levels than females with a median of 334 versus 158 U/L in transgenic homozygous mice, respectively. Mice were transplanted with human adult hepatocytes or fetal liver cells and analyzed for any correlation of engraftment of hepatocytes, liver sinusoidal endothelial cells (LSECs), and hematopoietic cells with the degree of liver damage...
2017: Cell Medicine
https://www.readbyqxmd.com/read/28713424/prolonged-survival-of-subcutaneous-allogeneic-islet-graft-by-donor-chimerism-without-immunosuppressive-treatment
#20
Brend Ray-Sea Hsu, Shin-Huei Fu, Aline Yen Ling Wang
The aim of this study was to investigate whether tolerance-induced protection of islets in the renal subcapsular space can also prevent subcutaneous allogeneic islets from being rejected. We used bone marrow stem cells from C57BL/6 (H2(b)) mice to construct donor chimerism in conditioned diabetic BALB/c (H2(d)) mice and investigated the effect of donor chimerism on engraftment and survival of subcutaneously transplanted allogeneic islets in streptozotocin-induced diabetic mice. We also studied the anti-inflammatory effect of mesenchymal stem cell on islet engraftment...
2017: International Journal of Endocrinology
keyword
keyword
105775
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"