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Plasma membrane ceramide

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https://www.readbyqxmd.com/read/28795093/anandamide-ceramide-interactions-in-a-membrane-environment-molecular-dynamic-simulations-data
#1
Coralie Di Scala, Morgane Mazzarino, Nouara Yahi, Karine Varini, Nicolas Garmy, Jacques Fantini, Henri Chahinian
Anandamide is a lipid neurotransmitter that interacts with various plasma membrane lipids. The data here consists of molecular dynamics simulations of anandamide, C18-ceramide and cholesterol performed in vacuo and within a hydrated palmitoyl-oleoyl-phosphatidylcholine (POPC)/cholesterol membrane. Several models of anandamide/cholesterol and anandamide/ceramide complexes are presented. The energy of interaction and the nature of the intermolecular forces involved in each of these complexes are detailed. The impact of water molecules hydrating the POPC/cholesterol membrane for the stability of the anandamide/cholesterol and anandamide/ceramide complexes is also analyzed...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28747072/regulation-of-staphylococcus-aureus-infection-of-macrophages-by-cd44-reactive-oxygen-species-and-acid-sphingomyelinase
#2
Cao Li, Yuqing Wu, Veronique Orian-Rousseau, Yang Zhang, Erich Gulbins, Heike Grassme
AIMS: Staphylococcus aureus plays an important role in sepsis, pneumonia, and wound infections. Acid sphingomyelinase (Asm)-deficient mice are highly susceptible to pulmonary S. aureus infections. Here, we investigated the role of CD44 as a molecule that mediates important aspects of the infection of macrophages with S. aureus. RESULTS: We showed that CD44 activation by S. aureus stimulated Asm via the formation of reactive oxygen species (ROS), resulting in ceramide release, clustering of CD44 in ceramide-enriched membrane platforms, CD44/Asm-dependent activation of Rho family GTPases, translocation of phospho-ezrin/radixin/moesin to the plasma-membrane, and a rapid rearrangement of the actin cytoskeleton with cortical actin polymerization...
July 26, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28743366/the-enzymatic-sphingomyelin-to-ceramide-conversion-increases-the-shear-membrane-viscosity-at-the-air-water-interface
#3
REVIEW
Elisa R Catapano, Paolo Natale, Francisco Monroy, Iván López-Montero
Whereas most of lipids have viscous properties and they do not have significant elastic features, ceramides behave as very rigid solid assemblies, displaying viscoelastic behaviour at physiological temperatures. The present review addresses the surface rheology of lipid binary mixtures made of sphingomyelin and ceramide. However, ceramide is formed by the enzymatic cleavage of sphingomyelin in cell plasma membranes. The consequences of the enzymatically-driven ceramide formation involve mechanical alterations of the embedding membrane...
July 19, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28607453/specific-skeletal-muscle-sphingolipid-compounds-in-energy-expenditure-regulation-and-weight-gain-in-native-americans-of-southwestern-heritage
#4
S Heinitz, P Piaggi, K L Vinales, A Basolo, J Spranger, D Piomelli, J Krakoff, R Jumpertz von Schwartzenberg
BACKGROUND/OBJECTIVES: In animal models, a role in the regulation of energy expenditure (EE) has been ascribed to sphingolipids, active components of cell membranes participating in cellular signaling. In humans, it is unknown whether sphingolipids have a role in the modulation of EE and, consequently, influence weight gain. The present study investigated the putative association of EE and weight gain with sphingolipid levels in the human skeletal muscle, a component of fat-free mass (the strongest determinant of EE), in adipose tissue and plasma...
June 13, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28597878/incorporation-studies-of-clickable-ceramides-in-jurkat-cell-plasma-membranes
#5
T Walter, J Schlegel, A Burgert, A Kurz, J Seibel, M Sauer
The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues varied in the acyl chain length and the position of the functional group for click chemistry. Fluorescence microscopy studies including anisotropy and quenching experiments showed significant differences in the accessibility of the functional group indicating different incorporation properties into the plasma membrane.
June 9, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28591572/hepatic-diacylglycerol-associated-protein-kinase-c%C3%AE%C2%B5-translocation-links-hepatic-steatosis-to-hepatic-insulin-resistance-in-humans
#6
Kasper W Ter Horst, Pim W Gilijamse, Ruth I Versteeg, Mariette T Ackermans, Aart J Nederveen, Susanne E la Fleur, Johannes A Romijn, Max Nieuwdorp, Dongyan Zhang, Varman T Samuel, Daniel F Vatner, Kitt F Petersen, Gerald I Shulman, Mireille J Serlie
Hepatic lipid accumulation has been implicated in the development of insulin resistance, but translational evidence in humans is limited. We investigated the relationship between liver fat and tissue-specific insulin sensitivity in 133 obese subjects. Although the presence of hepatic steatosis in obese subjects was associated with hepatic, adipose tissue, and peripheral insulin resistance, we found that intrahepatic triglycerides were not strictly sufficient or essential for hepatic insulin resistance. Thus, to examine the molecular mechanisms that link hepatic steatosis to hepatic insulin resistance, we comprehensively analyzed liver biopsies from a subset of 29 subjects...
June 6, 2017: Cell Reports
https://www.readbyqxmd.com/read/28576432/anticeramide-antibody-and-butyrylcholinesterase-in-peripheral-neuropathies
#7
Aparna Sykam, V R Gutlapalli, Sandeep P Tenali, A K Meena, Priscilla Chandran, Sujai Suneetha, Lavanya M Suneetha
Ceramide is a glycosphingolipid, a component of nerve and non neuronal cell membrane and plays a role in maintaining the integrity of neuronal tissue. Butyrylcholinesterase (BChE) is a multifunctional enzyme, its involvement in neurodegenerative diseases has been well established. Anticeramide antibody (Ab-Cer) and enzyme BChE have been implicated in peripheral neuropathies. The present study investigates whether there is an association between Ab-Cer and BChE activities and peripheral neuropathies. Patients included: human immunodeficiency virus associated peripheral neuropathy (HIV-PN, n=39), paucibacillary leprosy (PB-L, n=36), multibacillary leprosy (MB-L, n=52), diabetic neuropathy (DN, n=22), demyelinating sensory motor polyneuropathy (DSMN, n=13) and chronic inflammatory demyelinating polyneuropathy (CIDP, n=10)...
August 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28544772/ganglioside-gm3-content-in-skeletal-muscles-is-increased-in-type-2-but-decreased-in-type-1-diabetes-rat-models-implications-of-glycosphingolipid-metabolism-in-pathophysiology-of-diabetes
#8
Josko Bozic, Anita Markotic, Vedrana Cikes-Culic, Anela Novak, Josip Andelo Borovac, Hrvoje Vucemilovic, Gorana Trgo, Tina Ticinovic Kurir
BACKGROUND: Ganglioside GM3 is an abundant constituent of the plasma membrane where its accumulation attenuates insulin receptor signaling. Considering the role of skeletal muscles for insulin-stimulated glucose uptake, the aim of this study was to determine the expression of GM3 and its precursors in skeletal muscle of diabetes mellitus type 1 (DM1) and type 2 (DM2) rat models. METHODS: Diabetes was induced by streptozotocin (55 mg/kg for DM1 and 35 mg/kg for DM2) injection to male Sprague-Dawley rats which were fed with normal pellet diet (DM1 type) or high-fat diet (DM2 type)...
May 22, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/28542880/suicidal-death-of-erythrocytes-in-cancer-and-its-chemotherapy-a-potential-target-in-the-treatment-of-tumor-associated-anemia
#9
REVIEW
Elisabeth Lang, Rosi Bissinger, Syed M Qadri, Florian Lang
In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis characterized by cell shrinkage and cell membrane scrambling. Eryptotic erythrocytes are rapidly cleared from circulating blood and may adhere to the vascular wall. Stimulation of eryptosis thus impairs microcirculation and leads to anemia as soon as the loss of erythrocytes cannot be fully compensated by enhanced erythropoiesis. Signaling stimulating eryptosis includes increase of cytosolic Ca(2+) -activity, ceramide, caspases, calpain, p38-kinase, protein-kinase C, Janus-activated kinase 3, casein-kinase 1α, and cyclin-dependent kinase 4...
May 23, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28468968/tolerogenic-versus-immunogenic-lipidomic-profiles-of-cd11c-immune-cells-and-control-of-immunogenic-dendritic-cell-ceramide-dynamics
#10
Carlos Ocaña-Morgner, Susanne Sales, Manuela Rothe, Andrej Shevchenko, Rolf Jessberger
Lipids affect the membrane properties determining essential biological processes. Earlier studies have suggested a role of switch-activated protein 70 (SWAP-70) in lipid raft formation of dendritic cells. We used lipidomics combined with genetic and biochemical assays to analyze the role of SWAP-70 in lipid dynamics. TLR activation using LPS as a ligand represented a pathogenic immunogenic stimulus, physical disruption of cell-cell contacts a tolerogenic stimulus. Physical disruption, but not LPS, caused an increase of phosphatidylcholine ether and cholesteryl esters in CD11c(+) immune cells...
June 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28411183/membrane-permeation-of-arginine-rich-cell-penetrating-peptides-independent-of-transmembrane-potential-as-a-function-of-lipid-composition-and-membrane-fluidity
#11
Rike Wallbrecher, Tobias Ackels, R Alis Olea, Marco J Klein, Lucie Caillon, Jürgen Schiller, Petra H Bovée-Geurts, Toin H van Kuppevelt, Anne S Ulrich, Marc Spehr, Merel J W Adjobo-Hermans, Roland Brock
Cell-penetrating peptides (CPPs) are prominent delivery vehicles to confer cellular entry of (bio-) macromolecules. Internalization efficiency and uptake mechanism depend, next to the type of CPP and cargo, also on cell type. Direct penetration of the plasma membrane is the preferred route of entry as this circumvents endolysosomal sequestration. However, the molecular parameters underlying this import mechanism are still poorly defined. Here, we make use of the frequently used HeLa and HEK cell lines to address the role of lipid composition and membrane potential...
June 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28409208/inhibitors-of-ceramide-de-novo-biosynthesis-rescue-damages-induced-by-cigarette-smoke-in-airways-epithelia
#12
Aida Zulueta, Anna Caretti, Giuseppe Matteo Campisi, Andrea Brizzolari, Jose Luis Abad, Rita Paroni, Paola Signorelli, Riccardo Ghidoni
Exposure to cigarette smoke represents the most important risk factor for the development of chronic obstructive pulmonary disease (COPD). COPD is characterized by chronic inflammation of the airways, imbalance of proteolytic activity resulting in the destruction of lung parenchyma, alveolar hypoxia, oxidative stress, and apoptosis. Sphingolipids are structural membrane components whose metabolism is altered during stress. Known as apoptosis and inflammation inducer, the sphingolipid ceramide was found to accumulate in COPD airways and its plasma concentration increased as well...
April 13, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28405720/ceramide-synthase-2-deficiency-aggravates-aom-dss-induced-colitis-in-mice-role-of-colon-barrier-integrity
#13
Stephanie Oertel, Klaus Scholich, Andreas Weigert, Dominique Thomas, Julia Schmetzer, Sandra Trautmann, Marthe-Susanna Wegner, Heinfried H Radeke, Natalie Filmann, Bernhard Brüne, Gerd Geisslinger, Irmgard Tegeder, Sabine Grösch
Loss of intestinal barrier functions is a hallmark of inflammatory bowel disease like ulcerative colitis. The molecular mechanisms are not well understood, but likely involve dysregulation of membrane composition, fluidity, and permeability, which are all essentially regulated by sphingolipids, including ceramides of different chain length and saturation. Here, we used a loss-of-function model (CerS2(+/+) and CerS2(-/-) mice) to investigate the impact of ceramide synthase 2, a key enzyme in the generation of very long-chain ceramides, in the dextran sodium salt (DSS) evoked model of UC...
August 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28379629/characterization-of-plasma-membrane-ceramides-by-super-resolution-microscopy
#14
Anne Burgert, Jan Schlegel, Jérôme Bécam, Sören Doose, Erhard Bieberich, Alexandra Schubert-Unkmeir, Markus Sauer
The sphingolipid ceramide regulates cellular processes such as differentiation, proliferation, growth arrest, and apoptosis. Ceramide-rich membrane areas promote structural changes within the plasma membrane that segregate membrane receptors and affect membrane curvature and vesicle formation, fusion, and trafficking. Ceramides were labeled by immunocytochemistry to visualize their distribution on the plasma membrane of different cells with virtually molecular resolution by direct stochastic optical reconstruction microscopy (dSTORM)...
May 22, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28336574/switching-head-group-selectivity-in-mammalian-sphingolipid-biosynthesis-by-active-site-engineering-of-sphingomyelin-synthases
#15
Matthijs Kol, Radhakrishnan Panatala, Mirjana Nordmann, Leoni Swart, Leonie van Suijlekom, Birol Cabukusta, Angelika Hilderink, Tanja Grabietz, John G M Mina, Pentti Somerharju, Sergei Korneev, Fikadu G Tafesse, Joost C M Holthuis
SM is a fundamental component of mammalian cell membranes that contributes to mechanical stability, signaling, and sorting. Its production involves the transfer of phosphocholine from phosphatidylcholine onto ceramide, a reaction catalyzed by SM synthase (SMS)1 in the Golgi and SMS2 at the plasma membrane. Mammalian cells also synthesize trace amounts of the SM analog, ceramide phosphoethanolamine (CPE), but the physiological relevance of CPE production is unclear. Previous work revealed that SMS2 is a bifunctional enzyme producing both SM and CPE, whereas a closely related enzyme, SMS-related protein (SMSr)/SAMD8, acts as a monofunctional CPE synthase in the endoplasmic reticulum...
May 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28275055/altered-expression-of-ganglioside-gm3-molecular-species-and-a-potential-regulatory-role-during-myoblast-differentiation
#16
Shinji Go, Shiori Go, Lucas Veillon, Maria Grazia Ciampa, Laura Mauri, Chihiro Sato, Ken Kitajima, Alessandro Prinetti, Sandro Sonnino, Jin-Ichi Inokuchi
Gangliosides (sialic acid-containing glycosphingolipids) help regulate many important biological processes, including cell proliferation, signal transduction, and differentiation, via formation of functional microdomains in plasma membranes. The structural diversity of gangliosides arises from both the ceramide moiety and glycan portion. Recently, differing molecular species of a given ganglioside are suggested to have distinct biological properties and regulate specific and distinct biological events. Elucidation of the function of each molecular species is important and will provide new insights into ganglioside biology...
April 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28238856/gemcitabine-kills-proliferating-endothelial-cells-exclusively-via-acid-sphingomyelinase-activation
#17
Albert J van Hell, Adriana Haimovitz-Friedman, Zvi Fuks, William D Tap, Richard Kolesnick
Gemcitabine is a widely-used anti-cancer drug with a well-defined mechanism of action in normal and transformed epithelial cells. However, its effect on endothelial cells is largely unknown. Acid sphingomyelinase (ASMase) is highly expressed in endothelial cells, converting plasma membrane sphingomyelin to pro-apoptotic ceramide upon activation by diverse stresses. In the current study, we investigated gemcitabine impact in primary cultures of endothelial cells. We find baseline ASMase increases markedly in bovine aortic endothelial cells (BAEC) as they transit from a proliferative to a confluent growth-arrested state...
June 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28223543/role-of-caspases-in-cd95-induced-biphasic-activation-of-acid-sphingomyelinase
#18
Mario Stephan, Bärbel Edelmann, Supandi Winoto-Morbach, Ottmar Janssen, Uwe Bertsch, Cristiana Perrotta, Stefan Schütze, Jürgen Fritsch
Acid sphingomyelinase (A-SMase) plays an important role in the initiation of CD95 signaling by forming ceramide-enriched membrane domains that enable clustering and activation of the death receptors. In TNF-R1 and TRAIL-R1/R2 signaling, A-SMase also contributes to the lysosomal apoptosis pathway triggered by receptor internalization. Here, we investigated the molecular mechanism of CD95-mediated A-SMase activation, demonstrating that A-SMase is located in internalized CD95-receptosomes and is activated by the CD95/CD95L complex in a biphasic manner...
March 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28214863/stimulation-of-erythrocyte-cell-membrane-scrambling-by-c-reactive-protein
#19
Majed Abed, Christian Thiel, Syeda T Towhid, Kousi Alzoubi, Sabina Honisch, Florian Lang, Alfred Königsrainer
BACKGROUND: Eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine-translocation, is triggered by fever and inflammation. Signaling includes increased cytosolic Ca2+-activity ([Ca2+]i), caspase activation, and ceramide. Inflammation is associated with increased plasma concentration of C-reactive protein (CRP). The present study explored whether CRP triggers eryptosis. METHODS: Phosphatidylserine abundance at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, ceramide abundance and caspase-3-activity utilizing FITC-conjugated antibodies...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28179144/plasma-membrane-reorganization-links-acid-sphingomyelinase-ceramide-to-p38-mapk-pathways-in-endothelial-cells-apoptosis
#20
Colin Niaudet, Stéphanie Bonnaud, Maëva Guillonneau, Sébastien Gouard, Marie-Hélène Gaugler, Soizic Dutoit, Natacha Ripoche, Nolwenn Dubois, Valérie Trichet, Isabelle Corre, François Paris
The p38 MAPK signaling pathway is essential in the cellular response to stress stimuli, in particular in the endothelial cells that are major target of external stress. The importance of the bioactive sphingolipid ceramide generated by acid sphingomyelinase is also firmly established in stress-induced endothelial apoptotic cell death. Despite a suggested link between the p38 MAPK and ceramide pathways, the exact molecular events of this connection remain elusive. In the present study, by using two different activators of p38 MAPK, namely anisomycin and ionizing radiation, we depicted how ceramide generated by acid sphingomyelinase was involved in p38 MAPK-dependent apoptosis of endothelial cells...
February 6, 2017: Cellular Signalling
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