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https://www.readbyqxmd.com/read/26724455/a-three-dimensional-micropatterned-tumor-model-for-breast-cancer-cell-migration-studies
#1
Nitish Peela, Feba S Sam, Wayne Christenson, Danh Truong, Adam W Watson, Ghassan Mouneimne, Robert Ros, Mehdi Nikkhah
Breast cancer cell invasion is a highly orchestrated process driven by a myriad of complex microenvironmental stimuli, making it difficult to isolate and assess the effects of biochemical or biophysical cues (i.e. tumor architecture, matrix stiffness) on disease progression. In this regard, physiologically relevant tumor models are becoming instrumental to perform studies of cancer cell invasion within well-controlled conditions. Herein, we explored the use of photocrosslinkable hydrogels and a novel, two-step photolithography technique to microengineer a 3D breast tumor model...
March 2016: Biomaterials
https://www.readbyqxmd.com/read/26436359/a-simple-diagnostic-index-comprising-epithelial-membrane-antigen-and-fibronectin-for-hepatocellular-carcinoma
#2
Abdelfattah M Attallah, Mohamed El-Far, Camelia A Abdel Malak, Khaled Farid, Mohamed M Omran, Raida S Yahya, Entsar A Saad, Mohamed S Albannan, Ahmed A Attallah, Mohamed A El Basuni, Islam S Ali, Safaa B Abed, Mohamed A El Naggar
UNLABELLED: Background and rationale for the study. Continuing search for suitable tumor-markers is of clinical value in managing patients with various malignancies. These markers may be presented as intracellular substances in tissues or may be released into the circulation and appear in serum. Therefore, this work is concerned with identification and quantitative determination of epithelial membrane antigen (EMA) and fibronectin and estimating their performances as surrogate markers for identifying hepatocellular carcinoma (HCC)...
November 2015: Annals of Hepatology
https://www.readbyqxmd.com/read/24811383/fragile-x-mental-retardation-protein-regulates-synaptic-and-behavioral-plasticity-to-repeated-cocaine-administration
#3
Laura N Smith, Jakub P Jedynak, Miles R Fontenot, Carly F Hale, Karen C Dietz, Makoto Taniguchi, Feba S Thomas, Benjamin C Zirlin, Shari G Birnbaum, Kimberly M Huber, Mark J Thomas, Christopher W Cowan
Repeated cocaine exposure causes persistent, maladaptive alterations in brain and behavior, and hope for effective therapeutics lies in understanding these processes. We describe here an essential role for fragile X mental retardation protein (FMRP), an RNA-binding protein and regulator of dendritic protein synthesis, in cocaine conditioned place preference, behavioral sensitization, and motor stereotypy. Cocaine reward deficits in FMRP-deficient mice stem from elevated mGluR5 (or GRM5) function, similar to a subset of fragile X symptoms, and do not extend to natural reward...
May 7, 2014: Neuron
https://www.readbyqxmd.com/read/24501639/prokaryotic-super-program-advisory-committee-doe-joint-genome-institute-walnut-creek-ca-march-27-2013
#4
George M Garrity, Jill Banfield, Jonathan Eisen, Niels van der Lelie, Trina McMahon, Doug Rusch, Edward Delong, Mary Ann Moran, Cameron Currie, Jed Furhman, Steve Hallam, Phil Hugenholtz, Nancy Moran, Karen Nelson, Richard Roberts, Ramunas Stepanauskas
The Prokaryotic Super Program Advisory Committee met on March 27, 2013 for their annual review the Prokaryotic Super Program at the DOE Joint Genome Institute. As is the case with any site visit or program review, the objective is to evaluate progress in meeting organizational objectives, provide feedback to from the user-community and to assist the JGI in formulating plans for the coming year. The advisors want to commend the JGI for its central role in developing new technologies and capabilities, and for catalyzing the formation of new collaborative user communities...
July 30, 2013: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/23923600/knowledge-and-attitude-of-nursing-personnel-regarding-patient-safety-and-rights-in-hospitals-of-delhi
#5
Feba Geevarghese
Nursing ethics apart, the increased awareness towards the treatment procedures being adopted and the standards of hygiene in the hospital setting necessitates proper knowledge and attitude of the personnel attending the patients. The present study, conducted in government and private hospitals of Delhi covered 200 samples (100 each from government and private), using Structured Knowledge Questionnaire. It was revealed that majority of nursing staff working in private hospitals had greater knowledge about patient safety and rights; they also had better patient-friendly attitude...
November 2012: Nursing Journal of India
https://www.readbyqxmd.com/read/22196984/the-stress-induced-cytokine-interleukin-6-decreases-the-inhibition-excitation-ratio-in-the-rat-temporal-cortex-via-trans-signaling
#6
Francisco Garcia-Oscos, Humberto Salgado, Shawn Hall, Feba Thomas, George E Farmer, Jorge Bermeo, Luis Charles Galindo, Ruben D Ramirez, Santosh D'Mello, Stefan Rose-John, Marco Atzori
BACKGROUND: Although it is known that stress elevates the levels of pro-inflammatory cytokines and promotes hyper-excitable central conditions, a causal relationship between these two factors has not yet been identified. Recent studies suggest that increases in interleukin 6 (IL-6) levels are specifically associated with stress. We hypothesized that IL-6 acutely and directly induces cortical hyper-excitability by altering the balance between synaptic excitation and inhibition. METHODS: We used patch-clamp to determine the effects of exogenous or endogenous IL-6 on electrically evoked postsynaptic currents on a cortical rat slice preparation...
April 1, 2012: Biological Psychiatry
https://www.readbyqxmd.com/read/22128537/effect-of-water-matrices-on-removal-of-veterinary-pharmaceuticals-by-nanofiltration-and-reverse-osmosis-membranes
#7
Davor Dolar, Ana Vuković, Danijela Asperger, Kresimir Kosutić
This study explored the removal of five veterinary pharmaceuticals (VPs) (sulfamethoxazole (SMETOX), trimethoprim (TMP), ciprofloxacin (CIPRO), dexamethasone (DEXA) and febantel (FEBA)) from different water matrices (Milli-Q water, model water, tap water and real pharmaceutical wastewater using four types of nanofiltration (NF) membranes (NF90, NF270, NF and HL) and two reverse osmosis (RO) membranes (LFC-1 and XLE). All VPs were added to different water matrices at a concentration of 10 mg/L. Rejections of VPs and water flux were measured...
2011: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/20206209/nicotinic-modulation-of-auditory-attentional-shift-in-the-rat
#8
Dewey C Brown, Justin A Nichols, Feba Thomas, Lu Dinh, Marco Atzori
Numerous studies have demonstrated cognitive improvements resulting from the application of nicotine, especially in those tasks aimed at measuring attention. While the neuro-pharmacological relationship between nicotine and acetylcholine-driven attentional processes has been examined, studies tend to focus on the duration of time in which a subject can attend to a specific stimulus or series of stimuli rather than on the subjects' adaptive attentional capabilities. The present study addresses the possibility that the cholinergic agonist nicotine could improve performance on a task testing the ability to shift attention between sensory modalities under both normal and pharmacologically impaired conditions...
July 11, 2010: Behavioural Brain Research
https://www.readbyqxmd.com/read/15925235/rapid-determination-of-the-antiparasitic-drugs-flubendazole-and-febantel-in-feeds-by-hplc-with-ultraviolet-detection
#9
Guglielmo Dusi, Valentina Gamba, Elena Faggionato
A rapid and very effective analytical procedure for the simultaneous HPLC determination of two anthelmintics, Flubendazole (FLUB) and Febantel (FEBA), in swine and poultry feeds was developed and tested. The ground feed samples were extracted using dimethylformamide. The extracts were directly analyzed, without any purification, on a reversed-phase ODS column (150 mm x 4.6 mm, 5 microm) with acetonitrile-phosphate buffer (pH 3; 0.017 M) as mobile phase. Ultraviolet detection of FLUB and FEBA was carried out at 300 nm...
June 15, 2005: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/15547678/adrenomedullin-and-endothelin-1-stimulate-in-vitro-expansion-of-cord-blood-hematopoietic-stem-cells
#10
Sergio De Angeli, Laura Del Pup, Erica Febas, Maria Teresa Conconi, Mara Tommasini, Rosa Di Liddo, Giovanna Albertin, Pier Paolo Parnigotto, Gastone G Nussdorfer
The improvement of techniques for in vitro expansion of cord blood (CB) hematopoietic stem cells (SCs) is, at present, one main task of tissue engineering. Hence, we investigated whether endothelin-1 (ET-1) and adrenomedullin (AM), two regulatory peptides exerting growth promoting action on several cell systems, favor the in vitro expansion of CB SCs in liquid culture. CB hematopoietic cell middle-term expansion was carried out in a stroma-free liquid culture medium in the presence of ET-1, AM and three different cytokine combinations...
December 2004: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/11406272/feba-a-gene-for-eukaryotic-translation-initiation-factor-4e-binding-protein-4e-bp-in-dictyostelium-discoideum
#11
COMPARATIVE STUDY
T Morio, H Yasukawa, H Urushihara, T Saito, H Ochiai, I Takeuchi, M Maeda, Y Tanaka
We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammalian 4E-BPs. Northern blot analysis revealed that febA was expressed at a high level in the vegetative growth phase but the level of expression decreased during late development. The gene was shown to be non-essential since disruption of the gene had no severe effect; the null mutant proliferated normally and formed normal fruiting bodies...
May 28, 2001: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/5684115/-irradiation-of-mitoticly-active-root-end-cells-of-vicia-feba-with-3-gev-photons
#12
F Stähler, F Zywietz, H A Künkel
No abstract text is available yet for this article.
July 1968: Strahlentherapie
https://www.readbyqxmd.com/read/2116124/isolation-and-characterization-of-feeding-behavior-mutants-in-drosophila-melanogaster
#13
N Fujishiro, H Kijima, Y Miyakawa
Feeding behavior mutants of the fruit fly Drosophila melanogaster having point-mutations were isolated. The defects were analyzed in order to study the mechanism of feeding behavior. Point-mutations named febB (three allele) and febA (three allele) were located between the X-chromosome markers cho and cv and between the markers cv and v, respectively. The febB mutants showed a higher acceptance threshold of the proboscis extension reflex than wild-type flies and showed a normal chemosensory response to the electrophysiological test...
May 1990: Behavior Genetics
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