Marina Martinez-Garcia, Pedro Robles Naharro, Marnie W Skinner, Kerstin A Baran, Laura I Lascarez-Lagunas, Saravanapriah Nadarajan, Nara Shin, Carlos G Silva-García, Takamune T Saito, Sara Beese-Sims, Brianna N Diaz-Pacheco, Elizaveta Berson, Ana B Castañer, Sarai Pacheco, Enrique Martinez-Perez, Philip W Jordan, Monica P Colaiácovo
Chromosome movements and licensing of synapsis must be tightly regulated during early meiosis to ensure accurate chromosome segregation and avoid aneuploidy, although how these steps are coordinated is not fully understood. Here we show that GRAS-1, the worm homolog of mammalian GRASP/Tamalin and CYTIP, coordinates early meiotic events with cytoskeletal forces outside the nucleus. GRAS-1 localizes close to the nuclear envelope (NE) in early prophase I and interacts with NE and cytoskeleton proteins. Delayed homologous chromosome pairing, synaptonemal complex (SC) assembly, and DNA double-strand break repair progression are partially rescued by the expression of human CYTIP in gras-1 mutants, supporting functional conservation...
February 21, 2023: PLoS Genetics