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https://www.readbyqxmd.com/read/28238868/seroprevalence-isolation-and-co-infection-of-multiple-toxoplasma-gondii-strains-in-individual-bobcats-lynx-rufus-from-mississippi-usa
#1
Shiv K Verma, Amy R Sweeny, Matthew J Lovallo, Rafael Calero-Bernal, Oliver C Kwok, Tiantian Jiang, Chunlei Su, Michael E Grigg, Jitender P Dubey
Toxoplasma gondii causes lifelong chronic infection in both feline definitive hosts and intermediate hosts. Multiple exposures to the parasite are likely to occur in nature due to high environmental contamination. Here, we present data of high seroprevalence and multiple T. gondii strain co-infections in individual bobcats (Lynx rufus). Unfrozen samples (blood, heart, tongue and feces) were collected from 35 free ranging wild bobcats from Mississippi, USA. Toxoplasma gondii antibodies were detected in serum by the modified agglutination test (1:≥200) in all 35 bobcats...
February 23, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28235974/acute-effects-of-glucagon-like-peptide-1-glp-19-36-amide-and-exenatide-on-mesenteric-blood-flow-cardiovascular-parameters-and-biomarkers-in-healthy-volunteers
#2
Lasse Bremholm, Ulrik B Andersen, Mads Hornum, Linda Hilsted, Simon Veedfald, Bolette Hartmann, Jens Juul Holst
Glucagon-like peptide-1 (GLP-1, GLP-17-36amide) and its sister peptide glucagon-like peptide 2 (GLP-2) influence numerous intestinal functions and GLP-2 greatly increases intestinal blood flow. We hypothesized that GLP-1 also stimulates intestinal blood flow and that this would impact on the overall digestive and cardiovascular effects of the hormone. To investigate the influence of GLP-1 receptor agonism on mesenteric and renal blood flow and cardiovascular parameters, we carried out a double-blinded randomized clinical trial...
February 2017: Physiological Reports
https://www.readbyqxmd.com/read/28231703/effects-of-water-addition-to-total-mixed-ration-on-water-intake-nutrient-digestibility-wool-cortisol-and-blood-indices-in-corriedale-ewes
#3
Jalil Ghassemi Nejad, Byong-Wan Kim, Bae-Hun Lee, Kyung Il Sung, Ji-Yung Kim
Objective: The objective of this study was to determine the effect of adding water to total mixed ration (TMR) on fresh water intake, nutrient digestibility, wool cortisol, and blood indices in Corriedale ewes under hot and humid conditions. Methods: Nine non-pregnant Corriedale ewes (ave. BW = 41 ± 3.5 kg) were individually fed diets based on maintenance requirements in metabolic crates. Ewes were assigned to three treatment groups according to a triplicate 3×3 Latin Square design for 3 periods of 21 days duration each (9 ewes per treatment, 27 replications)...
February 23, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28226409/effects-of-xylanase-supplementation-on-growth-performance-nutrient-digestibility-blood-parameters-fecal-microbiota-fecal-score-and-fecal-noxious-gas-emission-of-weaning-pigs-fed-corn-soybean-meal-based-diet
#4
Ruixia Lan, Tianshui Li, Inho Kim
This study was conducted to evaluate the effects of xylanase supplementation on nutrient digestibility, growth performance, blood parameters, fecal microflora shedding, fecal score and fecal noxious gas emission of weaning pigs fed corn-soybean meal based diet. A total of 150 weaning pigs with an average initial body weight (BW) of 7.85 ± 0.93 kg were randomly allocated to three treatments based on BW and sex (10 replicate pens with five pigs, two gilts and three barrows) were used in this 42-day trial...
February 22, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28226348/-the-key-points-of-prevention-for-special-surgical-complications-after-radical-operation-of-gastric-cancer
#5
Hao Xu, Weizhi Wang, Panyuan Li, Diancai Zhang, Li Yang, Zekuan Xu
Incidence of gastric cancer is high in China and standard radical operation is currently the main treatment for gastric cancer. Postoperative complications, especially some special complications, can directly affect the prognosis of patients, even result in the increase of mortality. But the incidences of these special complications are low, so these complications are often misdiagnosed and delayed in treatment owing to insufficient recognition of medical staff. These special complications include (1) Peterson hernia: It is an abdominal hernia developed in the space between Roux loop and transverse colon mesentery after Roux-Y reconstruction of digestive tract...
February 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28221766/proteoform-profile-mapping-of-the-human-serum-complement-component-c9-reveals-unexpected-new-features-of-n-o-and-c-glycosylation
#6
Vojtech Franc, Yang Yang, Albert J R Heck
The human complement C9 protein (~65 kDa) is a member of the complement pathway. It plays an essential role in the membrane attack complex (MAC), which forms a lethal pore on the cellular surface of pathogenic bacteria. Here, we charted in detail the structural micro-heterogeneity of C9 purified from human blood serum, using an integrative workflow combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The proteoform profile of C9 was acquired by high-resolution native mass spectrometry, which revealed the co-occurrence of ~50 distinct MS signals...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28216610/pulmonary-kaposi-sarcoma-with-osseous-metastases-in-an-human-immunodeficiency-virus-hiv-patient-a-remarkable-response-to-highly-active-antiretroviral-therapy
#7
Ahmed Dirweesh, Muhammad Yasir Khan, Shaikh Fawad Hamiz, Nigahus Karabulut
BACKGROUND Kaposi sarcoma (KS) is known to involve the mucocutaneous tissues and the aero-digestive tracts. In acquired immune deficiency syndrome (AIDS) patients, KS has an aggressive course and carries poor prognosis. We present a case of pulmonary KS with osseous metastases as the first presentation of human immunodeficiency virus (HIV) infection in a young male. The lesions impressively decreased in size and numbers following initiation of highly active antiretroviral therapy (HAART). CASE REPORT A 34-year-old heterosexual male presented with a one month history of cough and 15-20 pound weight loss within six months...
February 20, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28215154/stress-and-salivary-glands
#8
Beáta Kerémi, Anita Beck, Tibor Károly Fábián, Gábor Fábián, Géza Szabó, Ákos Nagy, Gábor Varga
Salivary glands produce a bicarbonate-rich fluid containing digestive and protective proteins and other components to be delivered into the gastrointestinal tract. Its function is under strict control of the autonomic nervous system. Salivary electrolyte and fluid secretion is primarily controlled by parasympathetic activity, while protein secretion is primaily triggered by sympathetic stimulation. Stress activates the hypothalamic - pituitary - adrenal axis. The peripheral limb of this axis is the efferent sympathetic/adrenomedullary system...
February 15, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28214066/identification-of-g-170g-a-and-g-332g-a-mutations-in-exon-3-of-leptin-gene-bcnl-and-cail-and-their-association-with-semen-quality-and-testicular-dimensions-in-sanjabi-rams
#9
Roya Bakhtiar, Alireza Abdolmohammadi, Hadi Hajarian, Zahra Nikousefat, Davood Kalantar-Neyestanaki
The purpose of this study was to investigate leptin gene polymorphisms and their relationships with the characteristics of sperm quality and testicular dimensions. Semen samples were collected from 96 Sanjabi rams during autumn and spring seasons over two years. Simultaneously, the dimensions of length, width and scrotal circumference were measured. Blood samples were taken from the jugular vein to extract DNA. PCR was performed to amplify a 463bp fragment including exon 3 of leptin gene. PCR products were digested by Bcnl and Cail restriction enzymes to identify 170G>A and 332G>A mutations in exon 3, respectively...
February 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28212491/food-ingestion-in-an-upright-sitting-position-increases-post-prandial-amino-acid-availability-when-compared-to-food-ingestion-in-a-lying-down-position
#10
Andrew M Holwerda, Kaatje Lenaerts, Jörgen Bierau, Will K W H Wodzig, Luc J C van Loon
Dietary protein digestion and absorption kinetics determine the post-prandial increase in muscle protein synthesis. We recently demonstrated that body position during feeding can modulate the post-prandial rise in plasma amino acid availability. Here we investigated whether protein ingestion in an upright sitting body position accelerates gastric emptying and improves dietary protein digestion and subsequent amino acid absorption compared with feeding in a supine lying body position. In a crossover design, eight young males (26±1 y, 24...
February 17, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28212301/the-phytocomplex-from-fucus-vesiculosus-and-ascophyllum-nodosum-controls-postprandial-plasma-glucose-levels-an-in-vitro-and-in-vivo-study-in-a-mouse-model-of-nash
#11
Daniela Gabbia, Stefano Dall'Acqua, Iole Maria Di Gangi, Sara Bogialli, Valentina Caputi, Laura Albertoni, Ilaria Marsilio, Nicola Paccagnella, Maria Carrara, Maria Cecilia Giron, Sara De Martin
Edible seaweeds have been consumed by Asian coastal communities since ancient times. Fucus vesiculosus and Ascophyllum nodosum extracts have been traditionally used for the treatment of obesity and several gastrointestinal diseases. We evaluated the ability of extracts obtained from these algae to inhibit the digestive enzymes α-amylase and α-glucosidase in vitro, and control postprandial plasma glucose levels in a mouse model of non-alcoholic steatohepatitis (NASH); a liver disease often preceding the development of Type 2 diabetes (T2DM)...
February 15, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28209316/intestinal-adaptations-after-bariatric-surgery-consequences-on-glucose-homeostasis
#12
REVIEW
Jean-Baptiste Cavin, André Bado, Maude Le Gall
The gastrointestinal (GI) tract can play a direct role in glucose homeostasis by modulating the digestion and absorption of carbohydrates and by producing the incretin hormones. In recent years, numerous studies have focused on intestinal adaptation following bariatric surgeries. Changes in the number of incretin (glucagon-like peptide 1 and glucose-dependent insulinotropic polypeptide) producing cells have been reported, which could result in the modified hormonal response seen after surgery. In addition, the rate of absorption and the intestinal regions exposed to sugars may affect the time course of appearance of glucose in the blood...
February 10, 2017: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/28208686/identification-of-autophagy-related-genes-and-their-regulatory-mirnas-associated-with-celiac-disease-in-children
#13
Sergio Comincini, Federico Manai, Cristina Meazza, Sara Pagani, Carolina Martinelli, Noemi Pasqua, Gloria Pelizzo, Marco Biggiogera, Mauro Bozzola
Celiac disease (CD) is a severe genetic autoimmune disorder, affecting about one in 100 people, where the ingestion of gluten leads to damage in the small intestine. Diagnosing CD is quite complex and requires blood tests and intestinal biopsy examinations. Controversy exists regarding making the diagnosis without biopsy, due to the large spectrum of manifesting symptoms; furthermore, small-intestinal gastroscopy examinations have a relatively complex management in the pediatric population. To identify novel molecular markers useful to increase the sensitivity and specificity in the diagnosis of pediatric CD patients, the expression levels of two key autophagy executor genes (ATG7 and BECN1) and their regulatory validated miRNAs (miR-17 and miR-30a, respectively) were analyzed by relative quantitative real-time-PCR on a cohort of confirmed CD patients compared to age-related controls...
February 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28208252/high-confidence-qualitative-identification-of-organophosphorus-nerve-agent-adducts-to-human-butyrylcholinesterase
#14
Thomas P Mathews, Melissa D Carter, Darryl Johnson, Samantha L Isenberg, Leigh Ann Graham, Jerry D Thomas, Rudolph C Johnson
In this study, a data-dependent, high-resolution tandem mass spectrometry (ddHRMS/MS) method capable of detecting all organophosphorus nerve agent (OPNA) adducts to human butyrylcholinesterase (BChE) was developed. After an exposure event, immunoprecipitation from blood with a BChE-specific antibody and digestion with pepsin produces a nine amino acid peptide containing the OPNA adduct. Signature product ions of this peptic BChE nonapeptide (FGES*AGAAS) offer a route to broadly screen for OPNA exposure. Taking this approach on an HRMS instrument identifies biomarkers, including unknowns, with high mass accuracy...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28203019/-investigation-of-risk-factors-of-acute-kidney-injury-after-off-pump-coronary-artery-bypass-grafting-and-3-years-follow-up
#15
X H Li, F Xiao, S Y Zhang
OBJECTIVE: To investigate the incidence rate and risk factors of acute kidney injury (AKI) after off-pump coronary artery bypass grafting (CABG),and to compare the effects of AKI on complications after operation and major adverse cardiovascular and cerebrovascular events (MACCE) after 3 years' follow-up. METHODS: In the study, 299 consecutive patients who underwent scheduled off-pump CABG from January 2010 to March 2012 were included. The patients were divided into AKI group with AKI and control group without AKI after operation...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28196225/blood-vessel-basement-membrane-alterations-in-human-retinal-microaneurysms-during-aging
#16
Mariana López-Luppo, Victor Nacher, David Ramos, Joana Catita, Marc Navarro, Ana Carretero, Alfonso Rodriguez-Baeza, Luísa Mendes-Jorge, Jesús Ruberte
Purpose: Microaneurysms, considered a hallmark of retinal vascular disease, are present in aged retinas. Here, the basement membrane of human retinal microaneurysms has been analyzed during aging. Methods: Retinas were obtained from 17 nondiabetic donors. Whole mount retinas and paraffin sections were marked immunohistochemically with antibodies against the main components of the blood basement membrane. Trypsin digestion and transmission electron microscopy also were performed...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28195969/acute-mesenteric-ischemia-among-postcardiac-surgery-patients-presenting-with-multiple-organ-failure
#17
Alexis Guillaume, Sébastien Pili-Floury, Sidney Chocron, Eric Delabrousse, Bénédicte De Parseval, Stephane Koch, Emmanuel Samain, Gilles Capellier, Gaël Piton
BACKGROUND: Acute mesenteric ischemia (AMI) is a rare but severe complication after cardiac surgery. However, AMI is likely to be more frequent in the subgroup of patients presenting with multiple organ failure after a cardiac surgery. The primary objective of this study was to identify AMI risk factors among patients requiring intensive care unit (ICU) admission after cardiac surgery. METHODS: Retrospective observational study of all the patients requiring admission to two ICUs in a large university hospital after a cardiac surgery procedure...
March 2017: Shock
https://www.readbyqxmd.com/read/28193252/proteases-of-haematophagous-arthropod-vectors-are-involved-in-blood-feeding-yolk-formation-and-immunity-a-review
#18
REVIEW
Paula Beatriz Santiago, Carla Nunes de Araújo, Flávia Nader Motta, Yanna Reis Praça, Sébastien Charneau, Izabela M Dourado Bastos, Jaime M Santana
Ticks, triatomines, mosquitoes and sand flies comprise a large number of haematophagous arthropods considered vectors of human infectious diseases. While consuming blood to obtain the nutrients necessary to carry on life functions, these insects can transmit pathogenic microorganisms to the vertebrate host. Among the molecules related to the blood-feeding habit, proteases play an essential role. In this review, we provide a panorama of proteases from arthropod vectors involved in haematophagy, in digestion, in egg development and in immunity...
February 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28191332/angiotensin-ii-type-1-receptor-gene-a1166c-polymorphism-was-not-associated-with-acute-coronary-syndrome-in-an-iranian-population
#19
Navid Delshad, Majid Ghayour-Mobarhan, Hamed Mirzaei, Kamal Razavi-Azarkhiavi, Mohsen Moohebati, Mitra Hassany, Jamal Kasaian, Mohammad Reza Etemadzadeh, Maryam Sadat Alavi, Javad Behravan
BACKGROUND: There are very limited data for Iranian populations on the predisposing genetic factors for acute coronary syndrome (ACS). OBJECTIVES: The objective of the present study was to investigate the association of the angiotensin II type 1 receptor (AT1R) gene polymorphism and ACS in an Iranian population. PATIENTS AND METHODS: This cross-sectional study was conducted among 263 subjects (97 men and 166 women). Patients (n = 128) aged 30 - 80 years with chest pain were recruited from the emergency department of Ghaem Hospital (Mashhad, Iran)...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28188891/3-2-deoxy-%C3%AE-d-erythro-pentafuranosyl-pyrimido-1-2-%C3%AE-purin-10-3h-one-deoxyguanosine-adducts-of-workers-exposed-to-asbestos-fibers
#20
Stefano Bonassi, Filippo Cellai, Armelle Munnia, Donatella Ugolini, Alfonso Cristaudo, Monica Neri, Mirta Milić, Alessandra Bonotti, Roger W Giese, Marco E M Peluso
Asbestos is the commercial name for a group of silicate minerals naturally occurring in the environment and widely used in the industry. Asbestos exposure has been associated with pulmonary fibrosis, mesothelioma, and malignancies, which may appear after a period of latency of 20-40 years. Mechanisms involved in the carcinogenic effects of asbestos are still not fully elucidated, although the oxidative stress theory suggests that phagocytic cells produce large amounts of reactive oxygen species, due to their inability to digest asbestos fiber...
February 7, 2017: Toxicology Letters
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