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Phosphoethanolamine

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https://www.readbyqxmd.com/read/28068143/adaptations-to-excess-choline-in-insulin-resistant-and-pcyt2-deficient-skeletal-muscle
#1
Adrian Taylor, Laila Cigana Schenkel, Maiya Yokich, Marica Bakovic
It was hypothesized that choline supplementation in insulin resistant (IR) CTP:phosphoethanolamine cytidylyltransferase deficient (Pcyt2(+/-)) mice would ameliorate muscle function by remodeling glucose and fatty acid (FA) metabolism. Pcyt2(+/-) mice either received no treatment or were allowed access to 2 mg/mL choline in drinking water for 4 weeks. Skeletal muscle was harvested from choline treated and untreated mice. Lipid analysis and metabolic gene expression and signaling pathways were compared between untreated Pcyt2(+/-) mice, treated Pcyt2(+/-) mice, and Pcyt2(+/+) mice...
September 6, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28059088/insights-into-the-mechanistic-basis-of-plasmid-mediated-colistin-resistance-from-crystal-structures-of-the-catalytic-domain-of-mcr-1
#2
Philip Hinchliffe, Qiu E Yang, Edward Portal, Tom Young, Hui Li, Catherine L Tooke, Maria J Carvalho, Neil G Paterson, Jürgen Brem, Pannika R Niumsup, Uttapoln Tansawai, Lei Lei, Mei Li, Zhangqi Shen, Yang Wang, Christopher J Schofield, Adrian J Mulholland, Jianzhong Shen, Natalie Fey, Timothy R Walsh, James Spencer
The polymixin colistin is a "last line" antibiotic against extensively-resistant Gram-negative bacteria. Recently, the mcr-1 gene was identified as a plasmid-mediated resistance mechanism in human and animal Enterobacteriaceae, with a wide geographical distribution and many producer strains resistant to multiple other antibiotics. mcr-1 encodes a membrane-bound enzyme catalysing phosphoethanolamine transfer onto bacterial lipid A. Here we present crystal structures revealing the MCR-1 periplasmic, catalytic domain to be a zinc metalloprotein with an alkaline phosphatase/sulphatase fold containing three disulphide bonds...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28050958/non-invasive-prediction-of-blastocyst-implantation-ongoing-pregnancy-and-live-birth-by-mass-spectrometry-lipid-fingerprinting
#3
Edson Borges, Daniela P A F Braga, Amanda Souza Setti, Daniela A Montanni, Elaine Cristina Cabral, Marcos N Eberlin, Edson G Lo Turco, Assumpto Iaconelli
OBJECTIVE: To identify lipid markers of blastocyst implantation and ongoing pregnancy by day three culture medium mass spectrometry (MS) fingerprinting. METHODS: For this study, 33 culture media samples were harvested on day three, from 22 patients undergoing day five embryo transfers. All embryos achieved the blastocyst stage and were split into groups based on their implantation (Negative Implantation, n= 14 and Positive Implantation, n= 19). The positive implantation cycles resulted in successful ongoing pregnancies...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28032377/proton-and-phosphorus-magnetic-resonance-spectroscopy-of-the-healthy-human-breast-at-7%C3%A2-t
#4
Wybe J M van der Kemp, Bertine L Stehouwer, Vincent O Boer, Peter R Luijten, Dennis W J Klomp, Jannie P Wijnen
In vivo water- and fat-suppressed (1) H magnetic resonance spectroscopy (MRS) and (31) P magnetic resonance adiabatic multi-echo spectroscopic imaging were performed at 7 T in duplicate in healthy fibroglandular breast tissue of a group of eight volunteers. The transverse relaxation times of (31) P metabolites were determined, and the reproducibility of (1) H and (31) P MRS was investigated. The transverse relaxation times for phosphoethanolamine (PE) and phosphocholine (PC) were fitted bi-exponentially, with an added short T2 component of 20 ms for adenosine monophosphate, resulting in values of 199 ± 8 and 239 ± 14 ms, respectively...
December 28, 2016: NMR in Biomedicine
https://www.readbyqxmd.com/read/28027535/hyaluronic-acid-decorated-pluronic-p85-solid-lipid-nanoparticles-as-a-potential-carrier-to-overcome-multidrug-resistance-in-cervical-and-breast-cancer
#5
Fang Wang, Li Li, Bo Liu, Zhen Chen, Changzhong Li
This work aimed to develop hyaluronic acid (HA) decorated pluronic 85 (P85) coated solid lipid nanoparticles (SLN) loaded with paclitaxel (HA-PTX-P85-SLN) and to evaluate its potential to overcome drug resistance and to increase antitumor efficacy in mice bearing cervical and breast tumor. P85-Distearoyl Phosphoethanolamine (DSPE) was synthesized from P85 and DSPE by coupling in the presence of 1,10-carbonyldiimidazole (CDI) as a catalyst. The SLN were prepared by the hot homogenization technique and electrostatic interaction...
December 24, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28005445/preparation-and-in-vivo-safety-evaluations-of-antileukemic-homoharringtonine-loaded-pegylated-liposomes
#6
Dong Liu, Jing Xing, Fei Xiong, Fang Yang, Ning Gu
In order to improve the in vivo safety and specific delivery efficiency of the antileukemic homoharringtonine (HHT) at the targets, the long-circulating PEGylated liposomes loaded with HHT (LCLipo-HHT) were prepared. Their physical characteristics, in vitro drug release, in vivo pharmacokinetic properties and elementary toxicity were evaluated. The mean diameter of the prepared LCLipo-HHT is 75.6 ± 3.2 nm and the zeta potential is -16.9 ± 2.5 mV. The entrapment efficiency (EE) of HHT in the liposomes is 69...
December 22, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28001031/molecular-dynamics-simulation-and-experimental-studies-of-gold-nanoparticle-templated-hdl-like-nanoparticles-for-cholesterol-metabolism-therapeutics
#7
Cheng-Tsung Lai, Wangqiang Sun, Rohun U Palekar, C Shad Thaxton, George C Schatz
High-density lipoprotein (HDL) plays an important role in the transport and metabolism of cholesterol. Mimics of HDL are being explored as potentially powerful therapeutic agents for removing excess cholesterol from arterial plaques. Gold nanoparticles (AuNPs) functionalized with apolipoprotein A-I and with the lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio)propionate] have been demonstrated to be robust acceptors of cellular cholesterol...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28000749/high-resolution-crystal-structure-of-the-catalytic-domain-of-mcr-1
#8
Guixing Ma, Yifan Zhu, Zhicheng Yu, Ashfaq Ahmad, Hongmin Zhang
The newly identified mobile colistin resistant gene (mcr-1) rapidly spread among different bacterial strains and confers colistin resistance to its host, which has become a global concern. Based on sequence alignment, MCR-1 should be a phosphoethanolamine transferase, members of the YhjW/YjdB/YijP superfamily and catalyze the addition of phosphoethanolamine to lipid A, which needs to be validated experimentally. Here we report the first high-resolution crystal structure of the C-terminal catalytic domain of MCR-1 (MCR-1C) in its native state...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27999446/antimicrobial-activity-and-interactions-of-cationic-peptides-derived-from-galleria-mellonella-cecropin-d-like-peptide-with-model-membranes
#9
José Oñate-Garzón, Marcela Manrique-Moreno, Steven Trier, Chad Leidy, Rodrigo Torres, Edwin Patiño
Antimicrobial peptides are effector molecules of the innate immune system against invading pathogens. The cationic charge in their structures has a strong correlation with antimicrobial activity, being responsible for the initial electrostatic interaction between peptides and the anionic microbial surface. This paper contains evidence that charge modification in the neutral peptide Gm cecropin D-like (WT) improved the antimicrobial activity of the modified peptides. Two cationic peptides derived from WT sequence named as ΔM1 and ΔM2, with net charge of +5 and +9, respectively, showed at least an eightfold increase in their antimicrobial activity in comparison to WT...
December 21, 2016: Journal of Antibiotics
https://www.readbyqxmd.com/read/27998076/microwave-triggered-smart-drug-release-from-liposomes-co-encapsulating-doxorubicin-and-salt-for-local-combined-hyperthermia-and-chemotherapy-of-cancer
#10
Yushen Jin, Xiaolong Liang, Yunkun An, Zhifei Dai
The microwave and temperature sensitive liposomes were fabricated successfully from 1,2-dipalmityol-sn-glycero-3-phosphocholine (DPPC), cholesterol, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG2000) with a molar ratio of 4:1:0.26 by co-encapsulating NaCl and doxorubicin (DOX) through the thin-film hydration method to externally manipulate drug release at a predetermined location in the body at a desired time in the right dosage for combination microwave hyperthermia and chemotherapy of cancer to afford a synergistic therapeutic effect...
December 21, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27993732/fast-therapeutic-dna-internalization-a-high-potential-transfection-system-based-on-a-peptide-mimicking-cationic-lipid
#11
Christopher Janich, Shashank Reddy Pinnapireddy, Frank Erdmann, Thomas Groth, Andreas Langner, Udo Bakowsky, Christian Wölk
The delivery of nucleic acids into cells is a determining factor for successful gene therapy. In this study we investigate the uptake and time dependent processing of a lipid-based non-viral nucleic acid delivery system composed of a peptide-mimicking cationic lipid (N-{6-amino-1-[N-(9Z)-octadec-9-enylamino]-1-oxohexan-(2S)-2-yl}-N'-{2-[N,N-bis(2-aminoethyl)amino]ethyl}-2-hexadecylpropandiamide - OH4) and a phospholipid (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine - DOPE). Studies by confocal laser scanning microscopy (CLSM) indicate a rapid internalization of fluorescent labelled DNA within 1h...
December 18, 2016: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27958270/crystal-structure-of-escherichia-coli-originated-mcr-1-a-phosphoethanolamine-transferase-for-colistin-resistance
#12
Menglong Hu, Jiubiao Guo, Qipeng Cheng, Zhiqiang Yang, Edward Wai Chi Chan, Sheng Chen, Quan Hao
MCR-1 is a phosphoethanolamine (pEtN) transferase that modifies the pEtN moiety of lipid A, conferring resistance to colistin, which is an antibiotic belonging to the class of polypeptide antibiotics known as polymyxins and is the last-line antibiotic used to treat multidrug resistant bacterial infections. Here we determined the crystal structure of the catalytic domain of MCR-1 (MCR-1-ED), which is originated in Escherichia coli (E. coli). MCR-1-ED was found to comprise several classical β-α-β-α motifs that constitute a "sandwich" conformation...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27927196/the-topology-structure-and-pe-interaction-of-litaf-underpin-a-charcot-marie-tooth-disease-type-1c
#13
Anita K Ho, Jane L Wagstaff, Paul T Manna, Lena Wartosch, Seema Qamar, Elspeth F Garman, Stefan M V Freund, Rhys C Roberts
BACKGROUND: Mutations in Lipopolysaccharide-induced tumour necrosis factor-α factor (LITAF) cause the autosomal dominant inherited peripheral neuropathy, Charcot-Marie-Tooth disease type 1C (CMT1C). LITAF encodes a 17 kDa protein containing an N-terminal proline-rich region followed by an evolutionarily-conserved C-terminal 'LITAF domain', which contains all reported CMT1C-associated pathogenic mutations. RESULTS: Here, we report the first structural characterisation of LITAF using biochemical, cell biological, biophysical and NMR spectroscopic approaches...
December 7, 2016: BMC Biology
https://www.readbyqxmd.com/read/27917409/gadolinium-functionalized-peptide-amphiphile-micelles-for-multimodal-imaging-of-atherosclerotic-lesions
#14
Sang Pil Yoo, Federico Pineda, John C Barrett, Christopher Poon, Matthew Tirrell, Eun Ji Chung
The leading causes of morbidity and mortality globally are cardiovascular diseases, and nanomedicine can provide many improvements including disease-specific targeting, early detection, and local delivery of diagnostic agents. To this end, we designed fibrin-binding, peptide amphiphile micelles (PAMs), achieved by incorporating the targeting peptide cysteine-arginine-glutamic acid-lysine-alanine (CREKA), with two types of amphiphilic molecules containing the gadoliniuim (Gd) chelator diethylenetriaminepentaacetic acid (DTPA), DTPA-bis(stearylamide)(Gd), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[(poly(ethylene glycol) (PEG))-2000]-DTPA(Gd) (DSPE-PEG2000-DTPA(Gd))...
November 30, 2016: ACS Omega
https://www.readbyqxmd.com/read/27899954/phosphoethanolamine-and-the-danger-of-unproven-drugs
#15
Noam Pondé, Evandro de Azambuja, Felipe Ades
The use of unproven forms of therapy in cancer treatment is very common. In Brazil, the distribution by researchers to patients of an investigational agent called phophoethanolamine (PHOS) has led to a widely publicized scientific scandal. PHOS is a precursor to components of the cell membrane, with some published pre-clinical studies suggesting cytotoxic activity in cancer cells. The willingness of courts and of legislators to guarantee access to PHOS in spite of the lack of any clinical data and against the recommendations of scientific and medical organisations underscores the risks that unproven agents pose to regulatory authorities, health care systems and patients, and bears resemblance to other cases such as the controversy surrounding the approval of zidovudine for AIDS treatment by the FDA...
2016: Ecancermedicalscience
https://www.readbyqxmd.com/read/27893806/hplc-based-mass-spectrometry-characterizes-the-phospholipid-alterations-in-ether-linked-lipid-deficiency-models-following-oxidative-stress
#16
Robin Drechsler, Shaw-Wen Chen, Blair C R Dancy, Lena Mehrabkhani, Carissa Perez Olsen
Despite the fact that the discovery of ether-linked phospholipids occurred nearly a century ago, many unanswered questions remain concerning these unique lipids. Here, we characterize the ether-linked lipids of the nematode with HPLC-MS/MS and find that more than half of the phosphoethanolamine-containing lipids are ether-linked, a distribution similar to that found in mammalian membranes. To explore the biological role of ether lipids in vivo, we target fatty acyl-CoA reductase (fard-1), an essential enzyme in ether lipid synthesis, with two distinct RNAi strategies...
2016: PloS One
https://www.readbyqxmd.com/read/27877240/ros-responsive-mitochondria-targeting-blended-nanoparticles-chemo-and-photodynamic-synergistic-therapy-for-lung-cancer-with-on-demand-drug-release-upon-irradiation-with-a-single-light-source
#17
Caixia Yue, Yuming Yang, Chunlei Zhang, Gabriel Alfranca, Shangli Cheng, Lijun Ma, Yanlei Liu, Xiao Zhi, Jian Ni, Weihua Jiang, Jie Song, Jesús M de la Fuente, Daxiang Cui
Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for cancer. Here we report, for the first time, synthesis of a new mitochondria-targeting drug delivery system, ZnPc/CPT-TPPNPs. To synthesize this novel compound, polyethylene glycol was functionalized with thioketal linker-modified camptothecin (TL-CPT) and triphenylphosphonium to form the block copolymer, TL-CPT-PEG1K-TPP...
2016: Theranostics
https://www.readbyqxmd.com/read/27863688/determination-of-the-activity-of-maleimide-functionalized-phospholipids-during-preparation-of-liposomes
#18
Mira Oswald, Simon Geissler, Achim Goepferich
Numerous examples exist in the literature for the use of maleimide-thiol-reactions in the area of functionalized nanoparticles. Although the hydrolysis tendency of maleimides is well-known, qualitative and quantitative information on the stability and reactivity of maleimide groups during preparation and in final formulations are missing. This is surprising, since hydrolysis of maleimides prevents nanoparticle functionalization and results in an increase of negative surface charge due to the hydrolysis product maleic acid...
November 30, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27863184/lipase-sensitive-transfersomes-based-on-photosensitizer-polymerizable-lipid-conjugate-for-selective-antimicrobial-photodynamic-therapy-of-acne
#19
Hyung Park, Jonghwan Lee, Songhee Jeong, Byeong Nam Im, Min-Kyoung Kim, Su-Geun Yang, Kun Na
Acne vulgaris is a common skin problem affecting nearly 90% of adolescents and its development is associated with a colonization of Propionibacterium acnes (P. acnes). Although antibiotics have commonly been used to treat acne, antibiotic resistance of P. acnes is an emerging issue to be solved. In this study, a new way of photodynamic acne therapy is developed using P. acnes lipase-sensitive transfersome (DSPE-PEG-Pheo A (DPP) transfersome). For enhanced selectivity and skin penetration efficiency, DPP transfersomes are prepared from 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000], pheophorbide A (Pheo A), cholesterol, and Tween-80...
December 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27843311/cd20-monoclonal-antibody-targeted-nanoscale-drug-delivery-system-for-doxorubicin-chemotherapy-an-in-vitro-study-of-cell-lysis-of-cd20-positive-raji-cells
#20
Shuang Jiang, Xiaobo Wang, Zhiran Zhang, Lan Sun, Yunzhu Pu, Hongjuan Yao, Jingcao Li, Yan Liu, Yingge Zhang, Weijing Zhang
A monoclonal antibody targeted nanoscale drug delivery system (NDDS) for chemotherapy was evaluated in CD20-positive Raji cells in vitro. Nanoparticles were formed by the assembly of an amphiphilic polymer consisting of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxypolyethyleneglycol-2000 (DSPE-PEG2000). Active carbon nanoparticles (ACNP) were conjugated to the chemotherapeutic agent, doxorubicin (DOX), and the nanoliposome carrier, DSPE-PEG2000 and DSPE-PEG2000-NH2 conjugated to the human anti-CD20 monoclonal antibody that targets B-lymphocytes...
2016: International Journal of Nanomedicine
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