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Phosphoethanolamine

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https://www.readbyqxmd.com/read/28926278/planar-optical-nano-antennas-resolve-cholesterol-dependent-nanoscale-heterogeneities-in-the-plasma-membrane-of-living-cells
#1
Raju Regmi, Pamina Martina Winkler, Valentin Flauraud, Kyra Borgman, Carlo Manzo, Juergen Brugger, Herve Rigneault, Jerome Wenger, Maria F Garcia-Parajo
Optical nano-antennas can efficiently confine light into nanoscopic hotspots enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform to investigate the dynamics of individual biomolecules in living cell membranes and their partitioning into cholesterol-dependent lipid nanodomains. Here, we present optical nano-antenna arrays with accessible surface hotspots to study the characteristic diffusion dynamics of phosphoethanolamine (PE) and sphingomyelin (SM) in the plasma membrane of living cells at the nanoscale...
September 19, 2017: Nano Letters
https://www.readbyqxmd.com/read/28923651/high-resolution-magic-angle-spinning-nuclear-magnetic-resonance-spectroscopy-for-the-metabolic-assessment-of-acute-rejection-after-cardiac-transplantation-in-rats
#2
C W Lee, J S Lee, C W Woo, S Kim
PURPOSE: To evaluate the potential of high-resolution magic angle spinning (HR-MAS) (1)H nuclear magnetic resonance (NMR) spectroscopy for metabolite characterization and the differentiation of acute rejection after heart transplantation in rat models. METHODS: We transplanted syngeneic heart grafts from Lewis rats (n = 4) and allogeneic heart grafts from F344 rats (n = 4) heterotopically into Lewis recipients. On day 7 postoperatively, the transplanted hearts were harvested for ex vivo (1)H NMR spectroscopy and HR-MAS (1)H NMR spectroscopy...
October 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28912328/complete-genome-sequence-of-salmonella-enterica-subsp-enterica-serovar-paratyphi-b-sequence-type-28-harboring-mcr-1
#3
Maria Borowiak, Jens A Hammerl, Jennie Fischer, Istvan Szabo, Burkhard Malorny
In 2015, plasmid-mediated colistin resistance was reported to be caused by a mobilized phosphoethanolamine transferase gene (mcr-1) in Enterobacteriaceae Here, we announce the complete genome sequence of the earliest d-tartrate-fermenting Salmonella enterica subsp. enterica serovar Paratyphi B isolate harboring mcr-1 from the collection of the German National Reference Laboratory for Salmonella.
September 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28906060/novel-coordination-of-lipopolysaccharide-modifications-in-vibrio-cholerae-promotes-camp-resistance
#4
Carmen M Herrera, Jeremy C Henderson, Alexander A Crofts, M Stephen Trent
In the environment and during infection, the human intestinal pathogen Vibrio cholerae must overcome noxious compounds that damage the bacterial outer membrane. The El Tor and classical biotypes of O1 V. cholerae show striking differences in their resistance to membrane disrupting cationic antimicrobial peptides (CAMPs), such as polymyxins. The classical biotype is susceptible to CAMPs, but current pandemic El Tor biotype isolates gain CAMP resistance by altering the net charge of their cell surface through glycine modification of lipid A...
September 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28888701/direct-mass-spectrometry-differentiation-of-ectopic-and-eutopic-endometrium-in-patients-with-endometriosis
#5
Leila Adamyan, Natalia Starodubtseva, Anna Borisova, Assia Stepanian, Vitaliy Chagovets, Dinara Salimova, Zhihao Wang, Alexey Kononikhin, Igor Popov, Anna Bugrova, Konstantin Chingin, Andrey Kozachenko, Huanwen Chen, Vladimir Frankevich
STUDY OBJECTIVE: To introduce a method for rapid assessment of endometriotic tissues using direct mass spectrometry (MS)-based lipidomics. DESIGN: Prospective observational cohort study. DESIGN CLASSIFICATION: II 2. SETTING: Department of Operative Gynecology of the Research Centre for Obstetrics, Gynecology and Perinatology. PATIENTS: Fifty patients with ovarian cysts and peritoneal endometriosis who underwent laparoscopic surgery between 2014 and 2016...
September 6, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28874446/characterization-of-two-novel-lipopolysaccharide-phosphoethanolamine-transferases-in-pasteurella-multocida-and-their-role-in-resistance-to-cathelicidin-2
#6
Marina Harper, Amy Wright, Frank St Michael, Jianjun Li, Deanna Deveson Lucas, Mark Ford, Ben Adler, Andrew D Cox, John D Boyce
The lipopolysaccharide (LPS) produced by the Gram-negative bacterial pathogen Pasteurella multocida has phosphoethanolamine (PEtn) residues attached to lipid A, 3-deoxy-D-manno-octulosonic acid (Kdo), heptose and galactose. In this study, we show that PEtn is transferred to lipid A by the P. multocida EptA homologue, PetL, and is transferred to galactose by a novel PEtn transferase that is unique to P. multocida called PetG. Transcriptomic analyses indicated that petL expression was positively regulated by the global regulator Fis and negatively regulated by an Hfq-dependent small RNA...
September 5, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28867349/engineering-escherichia-coli-membrane-phospholipid-head-distribution-improves-tolerance-and-production-of-biorenewables
#7
Zaigao Tan, Pouyan Khakbaz, Yingxi Chen, Jeremy Lombardo, Jong Moon Yoon, Jacqueline V Shanks, Jeffery B Klauda, Laura R Jarboe
Economically competitive microbial production of biorenewable fuels and chemicals is often impeded by toxicity of the product to the microbe. Membrane damage is often identified as a major mechanism of this toxicity. Prior efforts to strengthen the microbial membrane by changing the phospholipid distribution have largely focused on the fatty acid tails. Herein, a novel strategy of phospholipid head engineering is demonstrated in Escherichia coli. Specifically, increasing the expression of phosphatidylserine synthase (+pssA) was found to significantly increase both the tolerance and production of octanoic acid, a representative membrane-damaging solvent...
September 1, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28864658/pla2-and-enpp6-may-act-in-concert-to-generate-phosphocholine-from-the-matrix-vesicle-membrane-during-skeletal-mineralization
#8
Alan J Stewart, Darren T K Leong, Colin Farquharson
Mineralization is a key process in the formation of bone and cartilage in vertebrates, involving the deposition of calcium and phosphate containing hydroxyapatite (HA) mineral within a collagenous matrix. Inorganic phosphate (Pi) accumulation within matrix vesicles (MVs) is a fundamental stage in the precipitation of HA, with PHOSPHO1 being identified as the principal enzyme acting to produce Pi PHOSPHO1 is a dual-specific phosphocholine/phosphoethanolamine phosphatase enriched in mineralizing cells and within MVs...
September 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28845737/biodistribution-study-of-99m-tc-gemcitabine-loaded-spherulites-in-sprague-dawley-rats-by-gamma-scintigraphy-to-investigate-its-lung-targeting-potential
#9
Rahul Dhande, Amit Tyagi, Rakesh Kumar Sharma, Hetal Thakkar
Gemcitabine Hydrochloride (GCH) is drug of choice for treatment of Non-Small Cell Lung Cancer. This project aims to formulate GCH loaded Spherulites for lung targeting using Soyabean Phosphatidylcholine, Cholesterol and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]. Vesicles were characterized for size, entrapment efficiency, drug release and in vitro cytotoxicity. Radiolabeling of GCH was done using reduced technetium-99m to study biodistribution in Sprague Dawley rats...
August 28, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28804244/development-of-new-contrast-agents-for-imaging-function-and-metabolism-by-magnetic-resonance-imaging
#10
Alexandra Carvalho, M Clara Gonçalves, M Luísa Corvo, M Bárbara F Martins
Liposomes are interesting nanosystems with a wide range of medical application. One particular application is their ability to enhance contrast in magnetic resonance images; when properly loaded with magnetic/superparamagnetic nanoparticles, this means to act as contrast agents. The design of liposomes loaded with magnetic particles, magnetoliposomes, presents a large number of possibilities depending on the application from image function to metabolism. More interesting is its double function application as theranostics (diagnostics and therapy)...
2017: Magnetic Resonance Insights
https://www.readbyqxmd.com/read/28780184/emergence-of-a-colistin-resistant-escherichia-coli-clinical-isolate-harboring-mcr-1-in-japan
#11
Tatsuya Tada, Kohei Uechi, Isamu Nakasone, Kayo Shimada, Masashi Nakamatsu, Teruo Kirikae, Jiro Fujita
The mcr-1 is a gene encoding a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. We describe here the emergence of a colistin-resistant Escherichia coli clinical isolate harboring plasmid-mediated mcr-1 in Japan. The isolate belonged to ST5702 and is suspected to come from livestock and transmitted to human. This is the first report of a clinical isolate harboring mcr-1 in Japan.
August 2, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28777086/1-12%C3%A2-%C3%A3-resolution-crystal-structure-of-the-catalytic-domain-of-the-plasmid-mediated-colistin-resistance-determinant-mcr-2
#12
Katie Coates, Timothy R Walsh, James Spencer, Philip Hinchliffe
MCR-2 confers resistance to colistin, a `last-line' antibiotic against extensively resistant Gram-negative pathogens. It is a plasmid-encoded phosphoethanolamine transferase that is closely related to MCR-1. To understand the diversity in the MCR family, the 1.12 Å resolution crystal structure of the catalytic domain of MCR-2 was determined. Variable amino acids are located distant from both the di-zinc active site and the membrane-proximal face. The exceptionally high resolution will provide an accurate starting model for further mechanistic studies...
August 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28763774/properties-of-popc-pope-supported-lipid-bilayers-modified-with-hydrophobic-quantum-dots-on-polyelectrolyte-cushions
#13
Marta Kolasinska-Sojka, Magdalena Wlodek, Michal Szuwarzynski, Sami Kereiche, Lubomir Kovacik, Piotr Warszynski
The formation and properties of supported lipid bilayers (SLB) containing hydrophobic nanoparticles (NP) was studied in relation to underlying cushion obtained from selected polyelectrolyte multilayers. Lipid vesicles were formed from zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and negatively charged 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) in phosphate buffer (PBS). As hydrophobic nanoparticles - quantum dots (QD) with size of 3.8nm (emission wavelength of 420nm) were used...
July 23, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28762066/zeta-potential-a-case-study-of-cationic-anionic-and-neutral-liposomes
#14
Mackensie C Smith, Rachael M Crist, Jeffrey D Clogston, Scott E McNeil
Zeta potential is often used to approximate a nanoparticle's surface charge, i.e., cationic, anionic, or neutral character, and has become a standard characterization technique to evaluate nanoparticle surfaces. While useful, zeta potential values provide only very general conclusions about surface charge character. Without a thorough understanding of the measurement parameters and limitations of the technique, these values can become meaningless. This case study attempts to explore the sensitivity of zeta potential measurement using specifically formulated cationic, anionic, and neutral liposomes...
July 31, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28756864/preparation-and-characterization-of-carbonic-anhydrase-conjugated-liposomes-for-catalytic-synthesis-of-calcium-carbonate-particles
#15
Keisuke Maeshima, Makoto Yoshimoto
The biomimetic approach using immobilized enzymes is useful for the synthesis of structurally defined inorganic materials. In this work, carbonic anhydrase (CA) from bovine erythrocytes was covalently conjugated at 25°C to the liposomes composed of 15mol% 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine-N-(glutaryl) (NG-POPE), and the zwitterionic and anionic phospholipids with the same acyl chains as NG-POPE. For the conjugation, the carboxyl groups of liposomal NG-POPE were activated with 11mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 4...
October 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28748758/protozoan-parasites-glycosylphosphatidylinositol-anchors-structures-functions-and-trends-for-drug-discovery
#16
Ana Luísa Malaco Morotti, Maristela Braga Martins-Teixeira, Ivone Carvalho
Glycosylphosphatidylinositol (GPI) anchors are complex molecules that support certain proteins in the outer leaflet of the cell membrane. The GPI anchor scaffold is comprised of a glycan core which contains a phosphoethanolamine linker and a phospholipid chain. GPI-anchored proteins are structurally and functionally diverse and play essential roles in several biological processes, in particular cell-cell interaction. Although all eukaryotes possess GPI anchors in their cell membrane, protozoan parasites use this anchorage much more frequently than higher eukaryotes...
July 27, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28731702/drude-polarizable-force-field-for-molecular-dynamics-simulations-of-saturated-and-unsaturated-zwitterionic-lipids
#17
Hui Li, Janamejaya Chowdhary, Lei Huang, Xibing He, Alexander D MacKerell, Benoît Roux
Additive force fields are designed to account for induced electronic polarization in a mean-field average way, using effective empirical fixed charges. The limitation of this approximation is cause for serious concerns, particularly in the case of lipid membranes, where the molecular environment undergoes dramatic variations over microscopic length scales. A polarizable force field based on the classical Drude oscillator offers a practical and computationally efficient framework for an improved representation of electrostatic interactions in molecular simulations...
September 12, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28724033/ethical-issues-on-the-synthetic-phosphoethanolamine-clinical-trial
#18
Francisco José Roma Paumgartten
Notwithstanding its approval by the National Committee for Ethics in Research (Conep) on April 19, 2016, a trial of the so-called "synthetic" phosphoethanolamine (syn-phospho) pill in cancer patients raises ethical concerns. An analysis by a laboratory contracted by the Ministry of Science, Technology and Innovation (MCTI) revealed that syn-phospho contained a great amount of impurities and did not meet standards of pharmaceutical quality required for an investigational drug. Cytotoxicity against human tumor cell lines and in vivo rodent xenograft tumor assays consistently failed to demonstrate a potential anticancer activity of syn-phospho...
May 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28683522/role-of-eptc-in-biofilm-formation-by-campylobacter-jejuni-nctc11168-on-polystyrene-and-glass-surfaces
#19
Eun Seob Lim, Joo-Sung Kim
The complex roles of cell surface modification in the biofilm formation of Campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are poorly understood. In a screen of mutants from random transposon mutagenesis, an insertional mutation in eptC gene (cj0256) resulted in a significant decrease in C. jejuni NCTC11168 biofilm formation (<20%) on major food contact surfaces, such as polystyrene and borosilicate glass, compared with wild-type cells (p < 0.05). In C. jejuni strain 81-176, the protein encoded by eptC modifies cell surface structures, such as lipid A, the inner core of lipooligosaccharide, and the flagellar rod protein (FlgG), by attaching phosphoethanolamine...
July 7, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28683347/composite-liposome-pei-nucleic-acid-lipopolyplexes-for-safe-and-efficient-gene-delivery-and-gene-knockdown
#20
Shashank Reddy Pinnapireddy, Lili Duse, Boris Strehlow, Jens Schäfer, Udo Bakowsky
Cytotoxicity is a major drawback impeding the therapeutic use of gene delivery and gene down-regulation vehicles. Apart from cytotoxicity, rapid degradation and low cellular uptake are other major factors affecting therapeutic use. Considering the above factors, formulation and development of PEI (Polyethylenimine) based, liposome encapsulated delivery vehicles with improved transfection efficiency and low cytotoxicity which can be used for gene delivery and gene knockdown. DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine), DPPC (Dipalmitoylphosphatidylcholine) and cholesterol have been considered as lipids of choice bearing in mind various factors such as rigidness and surface charge which greatly influence the formation of liposomes, polyplex encapsulation and transfection efficiency...
June 21, 2017: Colloids and Surfaces. B, Biointerfaces
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