keyword
MENU ▼
Read by QxMD icon Read
search

Phosphoethanolamine

keyword
https://www.readbyqxmd.com/read/29148380/high-rate-of-mcr-1-producing-escherichia-coli-and-klebsiella-pneumoniae-among-pigs-portugal
#1
Nicolas Kieffer, Marta Aires-de-Sousa, Patrice Nordmann, Laurent Poirel
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1-producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29146539/hyaluronan-decorated-liposomes-as-drug-delivery-systems-for-cutaneous-administration
#2
Silvia Franzé, Alessandro Marengo, Barbara Stella, Paola Minghetti, Silvia Arpicco, Francesco Cilurzo
The work aimed to evaluate the feasibility to design hyaluronic acid (HA) decorated flexible liposomes to enhance the skin penetration of nifedipine. Egg phosphatidylcholine (e-PC) based transfersomes (Tween 80) and transethosomes (ethanol) were prepared. HA was reacted with 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (HA-DPPE) and two molar ratios (0.5 and 3%) of conjugate with respect to e-PC were tested. The presence of HA significantly increased the packing order of the bilayer (as verified by differential scanning calorimetry), reducing both the encapsulation efficiency and the flexibility of the decorated liposomes in a dose-dependent manner...
November 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29138303/cxc-chemokines-exhibit-bactericidal-activity-against-multidrug-resistant-gram-negative-pathogens
#3
Matthew A Crawford, Debra J Fisher, Lisa M Leung, Sara Lomonaco, Christine Lascols, Antonio Cannatelli, Tommaso Giani, Gian Maria Rossolini, Yohei Doi, David R Goodlett, Marc W Allard, Shashi K Sharma, Erum Khan, Robert K Ernst, Molly A Hughes
The continued rise and spread of antimicrobial resistance among bacterial pathogens pose a serious challenge to global health. Countering antimicrobial-resistant pathogens requires a multifaceted effort that includes the discovery of novel therapeutic approaches. Here, we establish the capacity of the human CXC chemokines CXCL9 and CXCL10 to kill multidrug-resistant Gram-negative bacteria, including New Delhi metallo-beta-lactamase-1-producing Klebsiella pneumoniae and colistin-resistant members of the family Enterobacteriaceae that harbor the mobile colistin resistance protein MCR-1 and thus possess phosphoethanolamine-modified lipid A...
November 14, 2017: MBio
https://www.readbyqxmd.com/read/29131643/electrophysiology-of-epithelial-sodium-channel-enac-embedded-in-supported-lipid-bilayer-using-a-single-nanopore-chip
#4
Muhammad Shuja Khan, Noura Sayed Dosoky, Ghulam Mustafa, Darayas Patel, Bakhrom K Berdiev, John Dalton Williams
Nanopore-based technologies are highly adaptable supports for developing label-free sensor chips to characterize lipid bilayers, membrane proteins and nucleotides. We utilized a single nanopore chip to study electrophysiology of epithelial Na+ channel (ENaC) incorporated in supported lipid membrane (SLM). An isolated nanopore was developed inside the silicon cavity followed by fusing large unilamellar vesicles (LUVs) of DPPS (1,2-dipalmitoyl-sn-glycero-3-phosphoserine) and DPPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine) to produce a solvent-free SLM with giga-ohm (GΩ) sealed impedance...
November 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29120820/development-of-pegylated-aspartic-acid-modified-liposome-as-a-bone-targeting-carrier-for-the-delivery-of-paclitaxel-and-treatment-of-bone-metastasis
#5
Shugo Yamashita, Hidemasa Katsumi, Nozomi Hibino, Yugo Isobe, Yumiko Yagi, Yuka Tanaka, Saki Yamada, Chihiro Naito, Akira Yamamoto
To prevent bone metastasis, we developed polyethylene glycol (PEG)-conjugated aspartic acid (Asp)-modified liposomes (PEG-Asp-Lipo) as a bone-targeting carrier of paclitaxel (PTX) by using Asp-modified 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-Asp). The affinity of Asp-modified liposomes to hydroxyapatite increased as the concentration of DPPE-Asp increased. The bone accumulation of [(3)H]-labeled PEG(2)-Asp(33)-Lipo was approximately 24.6% 360 min after intravenous injection in mice, in contrast to 5...
October 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/29095596/1-6-diphenyl-1-3-5-hexatriene-dph-as-a-novel-matrix-for-maldi-ms-imaging-of-fatty-acids-phospholipids-and-sulfatides-in-brain-tissues
#6
Hanadi Ibrahim, Kristina Jurcic, Jasmine S-H Wang, Shawn Narain Whitehead, Ken K-C Yeung
1,6-Diphenyl-1,3,5-hexatriene (DPH) is a commonly-used fluorescence probe for studying cell membrane-lipids due to its affinity towards the acyl chains in the phospholipid bilayers. In this work, we investigated its use in matrix-assisted laser desorption/ionization (MALDI) as a new matrix for mass spectrometry imaging (MSI) of mouse and rat brain tissue. DPH exhibits very minimal matrix-induced background signals for the analysis of small molecules (below m/z of 1000). In the negative ion mode, DPH permits the highly sensitive detection of small fatty acids (m/z 200 to 350) as well as a variety of large lipids up to m/z of 1000, including lyso-phospholipid, phosphatidic acid (PA), phosphoethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), and sulfatides (ST)...
November 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29095434/a-new-molecular-mechanism-underlying-the-egcg-mediated-autophagic-modulation-of-afp-in-hepg2-cells
#7
Lin Zhao, Shengtang Liu, Jiaying Xu, Wei Li, Guangxin Duan, Haichao Wang, Huilin Yang, Zaixing Yang, Ruhong Zhou
Epigallocatechingallate (EGCG) is a major bioactive component of green tea and is associated with health benefits against multiple diseases including cancer. As an indicator of hepatocellular carcinoma (HCC), high levels of α-fetal protein (AFP) are related to malignant differentiation and poor prognosis of cancer cells. In this study, EGCG can effectively reduce AFP secretion and simultaneously induce AFP aggregation in human HCC HepG2 cells. EGCG-stimulated autophagy induces the degradation of AFP aggregates in HepG2 cells...
November 2, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29091227/mcr-1-and-mcr-2-variant-genes-identified-in-moraxella-species-isolated-from-pigs-in-great-britain-from-2014-to-2015
#8
Manal AbuOun, Emma J Stubberfield, Nick A Duggett, Miranda Kirchner, Luisa Dormer, Javier Nunez-Garcia, Luke P Randall, Fabrizio Lemma, Derrick W Crook, Christopher Teale, Richard P Smith, Muna F Anjum
Objectives: To determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain. Methods: Gram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS. Results: Variants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29089903/intravesicular-phosphatase-phospho1-function-in-enamel-mineralization-and-prism-formation
#9
Mirali Pandya, Lauren Rosene, Colin Farquharson, José L Millán, Thomas G H Diekwisch
The transport of mineral ions from the enamel organ-associated blood vessels to the developing enamel crystals involves complex cargo packaging and carriage mechanisms across several cell layers, including the ameloblast layer and the stratum intermedium. Previous studies have established PHOSPHO1 as a matrix vesicle membrane-associated phosphatase that interacts with matrix vesicles molecules phosphoethanolamine and phosphocholine to initiate apatite crystal formation inside of matrix vesicles in bone. In the present study, we sought to determine the function of Phospho1 during amelogenesis...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29079699/substrate-analog-interaction-with-mcr-1-offers-insight-into-the-rising-threat-of-the-plasmid-mediated-transferable-colistin-resistance
#10
Pengcheng Wei, Guangji Song, Mengyang Shi, Yafei Zhou, Yang Liu, Jun Lei, Peng Chen, Lei Yin
Colistin is considered a last-resort antibiotic against most gram-negative bacteria. Recent discoveries of a plasmid-mediated, transferable colistin-resistance gene (mcr-1) on all continents have heralded the imminent emergence of pan-drug-resistant superbacteria. The inner-membrane protein MCR-1 can catalyze the transfer of phosphoethanolamine (PEA) to lipid A, resulting in colistin resistance. However, little is known about the mechanism, and few drugs exist to address this issue. We present crystal structures revealing the MCR-1 catalytic domain (cMCR-1) as a monozinc metalloprotein with ethanolamine (ETA) and d-glucose, respectively, thus highlighting 2 possible substrate-binding pockets in the MCR-1-catalyzed PEA transfer reaction...
October 27, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29046930/asfotase-alfa-treatment-for-1%C3%A2-year-in-a-16%C3%A2-year-old-male-with-severe-childhood-hypophosphatasia
#11
S A Bowden, B H Adler
We describe the clinical outcome of asfotase alfa therapy in a 16-year-old boy with severe childhood hypophosphatasia (HPP), who began therapy at age 15 years. The patient was diagnosed with HPP at age 2 years when he presented with genu varum and premature loss of primary teeth. He had a history of multiple fractures requiring 16 orthopedic surgeries with rod and pin placement in his lower extremities. He had chronic skeletal pain and used cane to ambulate with great difficulty. His height Z score at age 15 years was - 5...
October 18, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/29038064/e-selectin-targeted-immunoliposomes-for-rapamycin-delivery-to-activated-endothelial-cells
#12
Shima Gholizadeh, Ganesh Ram R Visweswaran, Gert Storm, Wim E Hennink, Jan A A M Kamps, Robbert J Kok
Activated endothelial cells play a pivotal role in the pathology of inflammatory disorders and thus present a target for therapeutic intervention by drugs that intervene in inflammatory signaling cascades, such as rapamycin (mammalian target of rapamycin (mTOR) inhibitor). In this study we developed anti-E-selectin immunoliposomes for targeted delivery to E-selectin over-expressing tumor necrosis factor-α (TNF-α) activated endothelial cells. Liposomes composed of 1,2-dipalmitoyl-sn-glycero-3.;hosphocholine (DPPC), Cholesterol, and 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000]-maleimide (DSPE-PEG-Mal) were loaded with rapamycin via lipid film hydration, after which they were further functionalized by coupling N-succinimidyl-S-acetylthioacetate (SATA)-modified mouse anti human E-selectin antibodies to the distal ends of the maleimidyl (Mal)-PEG groups...
October 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29028412/the-bacteroid-periplasm-in-soybean-nodules-is-an-interkingdom-symbiotic-space
#13
Kent N Strodtman, Severin E Stevenson, James K Waters, Thomas P Mawhinney, Jay J Thelen, Joseph C Polacco, David W Emerich
The functional role of the periplasm of nitrogen-fixing bacteroids has not been determined. Proteins were isolated from the periplasm and cytoplasm of Bradyrhizobium diazoefficiens bacteroids and were analyzed using liquid chromatography tandem mass spectrometry proteomics. Identification of bacteroid periplasmic proteins was aided by periplasm prediction programs. Approximately 40% of all the proteins identified as periplasmic in the B. diazoefficiens genome were found expressed in the bacteroid form of the bacteria, indicating the periplasm is a metabolically active symbiotic space...
December 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29019691/ph-induced-changes-in-the-surface-viscosity-of-unsaturated-phospholipids-monitored-using-active-interfacial-microrheology
#14
Saba Ghazvini, Ryan Alonso, Nabil Alhakamy, Prajnaparamita Dhar
Lipid membranes, a major component of cells, are subjected to significant changes in pH depending on their location in the cell: the outer leaflet of the cell membrane is exposed to a pH of 7.4 whereas lipid membranes that make up late endosomes and lysosomes are exposed to a pH of as low as 4.4. The purpose of this study is to evaluate how changes in the environmental pH within cells alter the fluidity of phospholipid membranes. Specifically, we studied pH-induced alterations in the surface arrangement of monounsaturated lipids with zwitterionic headgroups (phosphoethanolamine (PE) and phosphocholine (PC)) that are abundant in plasma membranes as well as anionic lipids (phosphatidylserine (PS) and phosphatidylglycerol (PG)) that are abundant in inner membranes using a combination of techniques including surface tension vs area measurements, interfacial microrheology, and fluorescence/atomic force microscopy...
November 9, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28983683/in-vivo-metabolic-profiles-as-determined-by-31-p-and-short-te-1-h-mr-spectroscopy-no-difference-between-patients-with-idh-wildtype-and-idh-mutant-gliomas
#15
Katharina J Wenger, Elke Hattingen, Kea Franz, Joachim Steinbach, Oliver Bähr, Ulrich Pilatus
PURPOSE: Previous ex vivo spectroscopic data from tissue samples revealed differences in phospholipid metabolites between isocitrate dehydrogenase mutated (IDHmut) and IDH wildtype (IDHwt) gliomas. We investigated whether these changes can be found in vivo using (1)H-decoupled (31)P magnetic resonance spectroscopic imaging (MRSI) with 3D chemical shift imaging (CSI) at 3 T in patients with low and high-grade gliomas. METHODS: The study included 33 prospectively enrolled, mostly untreated patients who met spectral quality criteria according to the World Health Organization (WHO II n = 7, WHO III n = 17, WHO IV n = 9; 25 patients IDHmut, 8 patients IDHwt)...
October 5, 2017: Clinical Neuroradiology
https://www.readbyqxmd.com/read/28981289/using-curvature-power-to-map-the-domain-of-inverse-micellar-cubic-phases-the-case-of-aliphatic-aldehydes-in-1-2-dioleoyl-sn-glycero-3-phosphoethanolamine
#16
Jamie Burrell, Marcus K Dymond, Richard James Gillams, Duncan J Parker, G John Langley, Ana Maria Labrador, Tommy Nylander, George S Attard
Oxylipins, or fatty aldehydes, are a class of molecules produced from membrane lipids as a result of oxidative stress or enzyme-mediated peroxidation. Here we report the effects of two biologically important fatty aldehydes, trans, trans-2,4-decanedienal (DD) and cis-11-hexadecenal (HD), on the phase behaviour of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) in water. We compare the phase behaviour of DD/DOPE and HD/DOPE mixtures to the phase behaviour of oleic acid/ DOPE mixtures and show that DD, HD and oleic acid have similar effects on the phase diagrams of DOPE...
October 5, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28975359/urine-metabolomics-insight-into-acute-kidney-injury-point-to-oxidative-stress-disruptions-in-energy-generation-and-h2s-availability
#17
Marta Martin-Lorenzo, Laura Gonzalez-Calero, Angeles Ramos-Barron, Maria D Sanchez-Niño, Carlos Gomez-Alamillo, Juan Manuel García-Segura, Alberto Ortiz, Manuel Arias, Fernando Vivanco, Gloria Alvarez-Llamas
Acute kidney injury (AKI) is one of the main complications in acute care medicine and a risk factor for chronic kidney disease (CKD). AKI incidence has increased; however, its diagnosis has limitations and physiopathological mechanisms are underexplored. We investigated urine samples, aiming to identify major metabolite changes during human AKI evolution. Metabolic signatures found were further explored for a potential link to severity of injury. Twenty-four control subjects and 38 hospitalized patients with AKI were recruited and urine samples were collected at the time of diagnosis, during follow-up and at discharge...
December 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28962028/identification-of-a-novel-transposon-associated-phosphoethanolamine-transferase-gene-mcr-5-conferring-colistin-resistance-in-d-tartrate-fermenting-salmonella-enterica-subsp-enterica-serovar-paratyphi-b
#18
Maria Borowiak, Jennie Fischer, Jens A Hammerl, Rene S Hendriksen, Istvan Szabo, Burkhard Malorny
Objectives: Plasmid-mediated mobilized colistin resistance is currently known to be caused by phosphoethanolamine transferases termed MCR-1, MCR-2, MCR-3 and MCR-4. However, this study focuses on the dissection of a novel resistance mechanism in mcr-1 -, mcr-2 - and mcr-3 -negative d -tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B ( Salmonella Paratyphi B d Ta+) isolates with colistin MIC values >2 mg/L. Methods: A selected isolate from the strain collection of the German National Reference Laboratory for Salmonella was investigated by WGS and bioinformatical analysis to identify novel phosphoethanolamine transferase genes involved in colistin resistance...
September 18, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28960955/improving-tumor-specificity-and-anticancer-activity-of-dasatinib-by-dual-targeted-polymeric-micelles
#19
Qing Yao, Jong Hoon Choi, Zhi Dai, Jiao Wang, Dongin Kim, Xing Tang, Lin Zhu
To improve tumor targetability and drug efficacy and decrease drug resistance of dasatinib (DSB), the multifunctional micellar nanoparticles that combined the matrix metalloproteinase 2 (MMP2)-sensitive tumor (site) targeting with folate receptor-mediated tumor (cell) targeting were developed. Two major functional polymers, polyethylene glycol (5000 Da)-MMP2-sensitive peptide-phosphoethanolamine (PEG5k-pp-PE) and folic acid-polyethylene glycol (2000 Da)-phosphoethanolamine (FA-PEG2k-PE), were synthesized to construct the dual-targeted micellar nanoparticles (MMP/FR micelles)...
October 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28958150/synthesis-and-characterization-of-tethered-lipid-assemblies-for-membrane-protein-reconstitution-review
#20
Rémi Veneziano, Claire Rossi, Alexandre Chenal, Catherine Brenner, Daniel Ladant, Joël Chopineau
Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extracellular environment and intracellular membranes. Biological membranes also play a major role in metabolism regulation and cellular physiology (e.g., mitochondrial membranes). The elaboration of membrane based biomimetic systems allows us to reconstitute and investigate, in controlled conditions, biological events occurring at the membrane interface...
September 28, 2017: Biointerphases
keyword
keyword
105685
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"