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Phosphoethanolamine

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https://www.readbyqxmd.com/read/29348238/phosphoethanolamine-cellulose-a-naturally-produced-chemically-modified-cellulose
#1
Wiriya Thongsomboon, Diego O Serra, Alexandra Possling, Chris Hadjineophytou, Regine Hengge, Lynette Cegelski
Cellulose is a major contributor to the chemical and mechanical properties of plants and assumes structural roles in bacterial communities termed biofilms. We find that Escherichia coli produces chemically modified cellulose that is required for extracellular matrix assembly and biofilm architecture. Solid-state nuclear magnetic resonance spectroscopy of the intact and insoluble material elucidates the zwitterionic phosphoethanolamine modification that had evaded detection by conventional methods. Installation of the phosphoethanolamine group requires BcsG, a proposed phosphoethanolamine transferase, with biofilm-promoting cyclic diguanylate monophosphate input through a BcsE-BcsF-BcsG transmembrane signaling pathway...
January 19, 2018: Science
https://www.readbyqxmd.com/read/29342854/impact-of-prolonged-blood-incubation-and-extended-serum-storage-at-room-temperature-on-the-human-serum-metabolome
#2
Beate Kamlage, Sebastian Neuber, Bianca Bethan, Sandra González Maldonado, Antje Wagner-Golbs, Erik Peter, Oliver Schmitz, Philipp Schatz
Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along with quality assurance, methods to assess sample quality regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of the human serum metabolome to pre-analytical variations comprising prolonged blood incubation and extended serum storage at room temperature by using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) -based metabolomics...
January 13, 2018: Metabolites
https://www.readbyqxmd.com/read/29338975/critical-effects-of-polar-fluorescent-probes-on-the-interaction-of-dha-with-popc-supported-lipid-bilayers
#3
Kiera R Flynn, Alessandra Sutti, Lisandra L Martin, M Leigh Ackland, Angel A J Torriero
The understanding of lipid bilayer structure and function has been advanced by the application of molecular fluorophores. However, the effects of these probe molecules on the physicochemical properties of membranes being studied are poorly understood. A quartz crystal microbalance with dissipation monitoring instrument was used in this work to investigate the impact of two commonly used fluorescent probes, 1-palmitoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphocholine (NBD-PC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine-B-sulfonyl) (Lis-Rhod PE), on the formation and physicochemical properties of a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine supported lipid bilayer (POPC-SLB)...
January 12, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29330556/evolution-of-the-phosphatidylcholine-biosynthesis-pathways-in-green-algae-combinatorial-diversity-of-methyltransferases
#4
Takashi Hirashima, Masakazu Toyoshima, Takashi Moriyama, Naoki Sato
Phosphatidylcholine (PC) is one of the most common phospholipids in eukaryotes, although some green algae such as Chlamydomonas reinhardtii are known to lack PC. Recently, we detected PC in four species in the genus Chlamydomonas: C. applanata NIES-2202, C. asymmetrica NIES-2207, C. debaryana NIES-2212, and C. sphaeroides NIES-2242. To reveal the PC biosynthesis pathways in green algae and the evolutionary scenario involved in their diversity, we analyzed the PC biosynthesis genes in these four algae using draft genome sequences...
January 12, 2018: Journal of Molecular Evolution
https://www.readbyqxmd.com/read/29316727/cloning-and-functional-analysis-of-phosphoethanolamine-methyltransferase-promoter-from-maize-zea-mays-l
#5
Gai-Li Niu, Wei Gou, Xiang-Long Han, Cheng Qin, Li-Xin Zhang, Abd El-Fatah Abomohra, Muhammad Ashraf
Betaine, a non-toxic osmoprotectant, is believed to accumulate considerably in plants under stress conditions to maintain the osmotic pressure and promote a variety of processes involved in growth and development. Phosphoethanolamine N-methyltransferase (PEAMT), a key enzyme for betaine synthesis, is reported to be regulated by its upstream promoter. In the present investigation, by using the transgenic approach, a 1048 bp long promoter region of ZmPEAMT gene from Zea mays was cloned and functionally characterized in tobacco...
January 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29313660/enhancing-the-efficacy-and-safety-of-doxorubicin-against-hepatocellular-carcinoma-through-a-modular-assembly-approach-the-combination-of-polymeric-prodrug-design-nanoparticle-encapsulation-and-cancer-cell-specific-drug-targeting
#6
Li Xu, Shengjun Xu, Hangxiang Wang, Jun Zhang, Zun Chen, Linhui Pan, Jianguo Wang, Xuyong Wei, Hai-Yang Xie, Lin Zhou, Shusen Zheng, Xiao Xu
Intervention is urgently required to improve the therapeutic outcome for patients with unresectable hepatocellular carcinomas (HCCs). However, current chemotherapeutics, such as sorafenib and doxorubicin (DOX), provide only limited therapeutic benefits for this devastating disease. In this context, we present a modular assembly approach to the construction of a systemically injectable nanotherapeutic that can efficiently and safely deliver DOX in vivo. To achieve this goal, we covalently attached DOX to a polylactide (PLA) building block (Mw = 2600, n = 36), yielding DOX-PLA conjugate 1...
January 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29305141/dequalinium-based-functional-nanosomes-show-increased-mitochondria-targeting-and-anticancer-effect
#7
Yoonhee Bae, Min Kyo Jung, Seulgi Lee, Su Jeong Song, Ji Young Mun, Eric S Green, Jin Han, Kyung Soo Ko, Joon Sig Choi
Mitochondria are targets with great potential for therapeutics for many human disorders. However, drug delivery systems for such therapeutics remain in need of more efficient mitochondrial-targeting carriers. In this study, we report that nanosomes composed of Dequalinium/DOTAP (1,2-dioleoyl-3-trimethylammonium-propane)/DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), called DQA80s, can act in the dual role of mitochondrial-targeting carrier and anticancer agent for therapeutic interventions against mitochondrial diseases...
January 2, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29302821/optimization-of-weight-ratio-for-dspe-peg-tpgs-hybrid-micelles-to-improve-drug-retention-and-tumor-penetration
#8
Ya Jin, Zimei Wu, Caibin Li, Weisai Zhou, John P Shaw, Bruce C Baguley, Jianping Liu, Wenli Zhang
PURPOSE: To enhance therapeutic efficacy and prevent phlebitis caused by Asulacrine (ASL) precipitation post intravenous injection, ASL-loaded hybrid micelles with size below 40 nm were developed to improve drug retention and tumor penetration. METHODS: ASL-micelles were prepared using different weight ratios of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethyleneglycol-2000 (DSPE-PEG2000) and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) polymers...
January 4, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29288722/comparative-analysis-of-phosphoethanolamine-transferases-involved-in-polymyxin-resistance-across-ten-clinically-relevant-gram-negative-bacteria
#9
Jiayuan Huang, Yan Zhu, Meiling Han, Mengyao Li, Jiangning Song, Tony Velkov, Chen Li, Jian Li
The rapid emergence of Gram-negative 'superbugs' has become a significant threat to human health globally and polymyxins become a last-line therapy for these very problematic pathogens. Polymyxins exhibit their antibacterial killing by the initial interaction with lipid A in Gram-negative bacteria. Polymyxin resistance can be mediated by phosphoethanolamine (PEA) modification of lipid A that abolishes the initial electrostatic interaction with polymyxins. Both chromosome-encoded (e.g. EptA, EptB and EptC) and plasmid-encoded PEA transferases (e...
December 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29288241/covering-their-bases-the-phosphobase-methylation-pathway-in-plants
#10
Joseph J Barycki
Phosphoethanolamine methyltransferases add three methyl groups successively to their substrate to produce phosphocholine, an important precursor for phospholipid biosynthesis in diverse organisms. New work from Lee and Jez reveals critical domain movements that explain how multiple methylation reactions are uniquely coordinated by plant methyltransferases and provides insights into the evolution of this class of enzymes. As opposed to closely related family members, the intermediates in this pathway are likely shuttled between two tethered domains to ensure complete methylation...
December 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29247685/comparison-among-different-revealers-in-the-study-of-accelerated-blood-clearance-phenomenon
#11
Kaifan Liang, Lirong Wang, Yuqing Su, Mengyang Liu, Rui Feng, Yanzhi Song, Yihui Deng
The markers are the "revealers" of accelerated blood clearance (ABC) phenomenon. PEGylated nanocarriers labeled with various markers have been used to explore the mechanism of ABC. However, different markers were labeled on different nanocarriers, and the influence of different markers on ABC phenomenon is questionable. In this study, tocopheryl nicotinate (TN), N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (NBD-DPPE), and 1,1'-dioctadecyl-3,3,3',3'-tetra-methylindotricarbocyanine iodide (DiR) were selected as markers...
December 13, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29215148/31-p-t2-s-of-phosphomonoesters-phosphodiesters-and-inorganic-phosphate-in-the-human-brain-at-7t
#12
Wybe J M van der Kemp, Dennis W J Klomp, Jannie P Wijnen
PURPOSE: To determine the phosphorus-31 T2 s of phosphomonoesters, phosphodiesters, and inorganic phosphate in the healthy human brain at 7T. METHODS: A 3D chemical shift imaging multi-echo sequence with composite block pulses for refocusing was used to measure one free induction decay (FID) and seven full echoes with an echo spacing of 45 ms on the brain of nine healthy volunteers (age range 22-45 years; average age 27 ± 8 years). Spectral fitting was used to determine the change in metabolic signal amplitude with echo time...
December 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29190918/plasma-lipid-profiling-and-diagnostic-biomarkers-for-oral-squamous-cell-carcinoma
#13
Lina Wang, Xin Wang, Ying Li, Yan Hou, Fengyu Sun, Shuang Zhou, Chunming Li, Bin Zhang
Biological requirements for tumor cell proliferation include the sustained increase of structural, energetic, signal transduction and biosynthetic precursors. Because lipids participate in membrane construction, energy storage, and cell signaling. We hypothesized that the differences in lipids between malignant carcinoma and normal controls could be reflected in the bio-fluids. A total of 100 pre-operative plasma samples were collected from 50 oral squamous cell carcinoma (OSCC), 50 normal patients and characterize by lipid profiling using ultra performance liquid chromatography/electro spray ionization mass spectrometry (UPLC-MS)...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29172154/specific-binding-of-human-c-reactive-protein-towards-supported-monolayers-of-binary-and-engineered-phospholipids
#14
Tatsuro Goda, Yuji Miyahara
Circulating C-reactive protein (CRP) recognizes altered plasma membranes and activates complements systems in the acute phase of inflammation and infection in human. We have shown previously the calcium-independent adsorption of CRP toward 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and lysophosphatidylcholine (LPC) on supported phospholipid monolayers. Here, we extended our study to other phospholipids and additives to elucidate the pattern recognition of CRP using a surface plasmon resonance biosensor...
November 20, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29148380/high-rate-of-mcr-1-producing-escherichia-coli-and-klebsiella-pneumoniae-among-pigs-portugal
#15
Nicolas Kieffer, Marta Aires-de-Sousa, Patrice Nordmann, Laurent Poirel
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1-producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29146539/hyaluronan-decorated-liposomes-as-drug-delivery-systems-for-cutaneous-administration
#16
Silvia Franzé, Alessandro Marengo, Barbara Stella, Paola Minghetti, Silvia Arpicco, Francesco Cilurzo
The work aimed to evaluate the feasibility to design hyaluronic acid (HA) decorated flexible liposomes to enhance the skin penetration of nifedipine. Egg phosphatidylcholine (e-PC) based transfersomes (Tween 80) and transethosomes (ethanol) were prepared. HA was reacted with 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (HA-DPPE) and two molar ratios (0.5 and 3%) of conjugate with respect to e-PC were tested. The presence of HA significantly increased the packing order of the bilayer (as verified by differential scanning calorimetry), reducing both the encapsulation efficiency and the flexibility of the decorated liposomes in a dose-dependent manner...
November 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29138303/cxc-chemokines-exhibit-bactericidal-activity-against-multidrug-resistant-gram-negative-pathogens
#17
Matthew A Crawford, Debra J Fisher, Lisa M Leung, Sara Lomonaco, Christine Lascols, Antonio Cannatelli, Tommaso Giani, Gian Maria Rossolini, Yohei Doi, David R Goodlett, Marc W Allard, Shashi K Sharma, Erum Khan, Robert K Ernst, Molly A Hughes
The continued rise and spread of antimicrobial resistance among bacterial pathogens pose a serious challenge to global health. Countering antimicrobial-resistant pathogens requires a multifaceted effort that includes the discovery of novel therapeutic approaches. Here, we establish the capacity of the human CXC chemokines CXCL9 and CXCL10 to kill multidrug-resistant Gram-negative bacteria, including New Delhi metallo-beta-lactamase-1-producing Klebsiella pneumoniae and colistin-resistant members of the family Enterobacteriaceae that harbor the mobile colistin resistance protein MCR-1 and thus possess phosphoethanolamine-modified lipid A...
November 14, 2017: MBio
https://www.readbyqxmd.com/read/29131643/electrophysiology-of-epithelial-sodium-channel-enac-embedded-in-supported-lipid-bilayer-using-a-single-nanopore-chip
#18
Muhammad Shuja Khan, Noura Sayed Dosoky, Ghulam Mustafa, Darayas Patel, Bakhrom K Berdiev, John Dalton Williams
Nanopore-based technologies are highly adaptable supports for developing label-free sensor chips to characterize lipid bilayers, membrane proteins and nucleotides. We utilized a single nanopore chip to study electrophysiology of epithelial Na+ channel (ENaC) incorporated in supported lipid membrane (SLM). An isolated nanopore was developed inside the silicon cavity followed by fusing large unilamellar vesicles (LUVs) of DPPS (1,2-dipalmitoyl-sn-glycero-3-phosphoserine) and DPPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine) to produce a solvent-free SLM with giga-ohm (GΩ) sealed impedance...
November 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29120820/development-of-pegylated-aspartic-acid-modified-liposome-as-a-bone-targeting-carrier-for-the-delivery-of-paclitaxel-and-treatment-of-bone-metastasis
#19
Shugo Yamashita, Hidemasa Katsumi, Nozomi Hibino, Yugo Isobe, Yumiko Yagi, Yuka Tanaka, Saki Yamada, Chihiro Naito, Akira Yamamoto
To prevent bone metastasis, we developed polyethylene glycol (PEG)-conjugated aspartic acid (Asp)-modified liposomes (PEG-Asp-Lipo) as a bone-targeting carrier of paclitaxel (PTX) by using Asp-modified 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-Asp). The affinity of Asp-modified liposomes to hydroxyapatite increased as the concentration of DPPE-Asp increased. The bone accumulation of [3H]-labeled PEG(2)-Asp(33)-Lipo was approximately 24.6% 360 min after intravenous injection in mice, in contrast to 5...
October 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/29095596/1-6-diphenyl-1-3-5-hexatriene-dph-as-a-novel-matrix-for-maldi-ms-imaging-of-fatty-acids-phospholipids-and-sulfatides-in-brain-tissues
#20
Hanadi Ibrahim, Kristina Jurcic, Jasmine S-H Wang, Shawn Narain Whitehead, Ken K-C Yeung
1,6-Diphenyl-1,3,5-hexatriene (DPH) is a commonly-used fluorescence probe for studying cell membrane-lipids due to its affinity towards the acyl chains in the phospholipid bilayers. In this work, we investigated its use in matrix-assisted laser desorption/ionization (MALDI) as a new matrix for mass spectrometry imaging (MSI) of mouse and rat brain tissue. DPH exhibits very minimal matrix-induced background signals for the analysis of small molecules (below m/z of 1000). In the negative ion mode, DPH permits the highly sensitive detection of small fatty acids (m/z 200 to 350) as well as a variety of large lipids up to m/z of 1000, including lyso-phospholipid, phosphatidic acid (PA), phosphoethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylinositol (PI), and sulfatides (ST)...
November 2, 2017: Analytical Chemistry
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