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Ernst R Werner, Markus A Keller, Sabrina Sailer, Daniele Seppi, Georg Golderer, Gabriele Werner-Felmayer, Raphael A Zoeller, Katrin Watschinger
Plasmanylethanolamine desaturase (E.C. introduces the 1-prime double bond into plasmalogens, one of the most abundant phospholipids in the human body. This labile membrane enzyme has not been purified and its coding sequence is unknown. Previous assays for this enzyme used radiolabeled substrates followed by multistep processing. We describe here a straight-forward method for the quantification of plasmanylethanolamine desaturase in enzyme incubation mixtures using pyrene-labeled substrates and reversed phase high performance liquid chromatography (HPLC) with fluorescence detection...
March 14, 2018: Journal of Lipid Research
Marek J Noga, Ronald Zielman, Robin M van Dongen, Sabine Bos, Amy Harms, Gisela M Terwindt, Arn M J M van den Maagdenberg, Thomas Hankemeier, Michel D Ferrari
Introduction: Metabolic profiling of cerebrospinal fluid (CSF) is a promising technique for studying brain diseases. Measurements should reflect the in vivo situation, so ex vivo metabolism should be avoided. Objective: To investigate the effects of temperature (room temperature vs. 4 °C), centrifugation and ethanol, as anti-enzymatic additive during CSF sampling on concentrations of glutamic acid, glutamine and other endogenous amines. Methods: CSF samples from 21 individuals were processed using five different protocols...
2018: Metabolomics: Official Journal of the Metabolomic Society
William Gustavo Lima, Mara Cristina Alves, Waleska Stephanie Cruz, Magna Cristina Paiva
Acinetobacter baumannii is an opportunistic pathogen associated with nosocomial and community infections of great clinical relevance. Its ability to rapidly develop resistance to antimicrobials, especially carbapenems, has re-boosted the prescription and use of polymyxins. However, the emergence of strains resistant to these antimicrobials is becoming a critical issue in several regions of the world because very few of currently available antibiotics are effective in these cases. This review summarizes the most up-to-date knowledge about chromosomally encoded and plasmid-mediated polymyxins resistance in A...
March 9, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Sue C Nang, Faye C Morris, Michael J McDonald, Mei-Ling Han, Jiping Wang, Richard A Strugnell, Tony Velkov, Jian Li
Objectives: The discovery of mobile colistin resistance mcr-1, a plasmid-borne polymyxin resistance gene, highlights the potential for widespread resistance to the last-line polymyxins. In the present study, we investigated the impact of mcr-1 acquisition on polymyxin resistance and biological fitness in Klebsiella pneumoniae. Methods: K. pneumoniae B5055 was used as the parental strain for the construction of strains carrying vector only (pBBR1MCS-5) and mcr-1 recombinant plasmids (pmcr-1)...
March 5, 2018: Journal of Antimicrobial Chemotherapy
Yi-Hsiang Cheng, Tzu-Lung Lin, Yi-Tsung Lin, Jin-Town Wang
Background: Colistin is one of the last-resort antibiotics used to treat carbapenem-resistant Klebsiella pneumoniae infection. Our previous studies indicated that clinical strains encoding CrrB with amino acid substitutions exhibited higher colistin resistance (MICs ≥512 mg/L) than did colistin-resistant strains encoding mutant MgrB, PmrB or PhoQ. Objectives: CrrAB may regulate another unknown mechanism(s) contributing to colistin resistance, besides modifications of LPS with 4-amino-4-deoxy-l-arabinose and phosphoethanolamine...
February 28, 2018: Journal of Antimicrobial Chemotherapy
Robert G E Krause, J P Dean Goldring
BACKGROUND: Plasmodium knowlesi is recognised as the main cause of human malaria in Southeast Asia. The disease is often misdiagnosed as P. falciparum or P. malariae infections by microscopy, and the disease is difficult to eliminate due to its presence in both humans and monkeys. P. knowlesi infections can rapidly cause severe disease and require prompt diagnosis and treatment. No protein biomarker exists for the rapid diagnostic test (RDT) detection of P. knowlesi infections. Plasmodium knowlesi infections can be diagnosed by PCR...
2018: PloS One
Yasuhiro Horibata, Orly Elpeleg, Ayelet Eran, Yoshio Hirabayashi, David Savitzki, Galit Tal, Hanna Mandel, Hiroyuki Sugimoto
Ethanolamine phosphotransferase 1 (EPT1), also known as selenoprotein 1 (SELENOI), is an enzyme that transfers phosphoethanolamine from cytidine diphosphate (CDP)-ethanolamine to lipid acceptors to produce ethanolamine glycerophospholipids such as diacyl-linked phosphatidylethanolamine (PE) and ether-linked plasmalogen (plasmenyl-PE). However, to date there has been no analysis of the metabolomic consequence of the mutation of EPT1 on the concentration of ethanolamine glycerophospholipids in mammalian cells...
March 2, 2018: Journal of Lipid Research
Zerrin Sezgin-Bayindir, Ayse Eser Elcin, Mahmut Parmaksiz, Yasar Murat Elcin, Nilufer Yuksel
Medication during pregnancy is often a necessity for women to treat their acute or chronic diseases. The goal of this study is to evaluate the potential of micelle-like nanoparticles (MNP) for providing safe drug usage in pregnancy and protect both fetus and mother from medication side effects. Clonazepam loaded MNP were prepared from copolymers (polystyrene-poly(acrylic acid) (PS-PAA), poly(ethylene glycol)-b-poly(lactic acid) (PEG-PLA) and distearyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-poly(ethylene glycol) (PEG-DSPE)) with varying monomer ratios and their drug loading efficiency, drug release ratio, particle size, surface charge and morphology were characterized...
March 1, 2018: Journal of Microencapsulation
Roland Hillmann, Martina Viefhues, Lukas Gött-Zink, Dominic Gilzer, Thomas Hellweg, Armin Gölzhäuser, Tilman Kottke, Dario Anselmetti
Free-standing lipid membranes are promising for many biophysical applications as a model system to mimic biological cell membranes. To improve the mechanical properties of lipid membranes UV-polymerized lipids have been introduced. We investigated free-standing as well as substrate-supported monolayers of 1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (PTPE) and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) and characterized them with respect to their structure, morphology and stability...
February 27, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Usha Yadav, Mohd Ashraf Khan
The GPI (Glycosylphosphatidylinositol) biosynthetic pathway is a multistep conserved pathway in eukaryotes that culminates in the generation of GPI glycolipid which in turn anchors many proteins (GPI-APs) to the cell surface. In spite of the overall conservation of the pathway, there still exist subtle differences in the GPI pathway of mammals and other eukaryotes which holds a great promise so far as the development of drugs/inhibitors against specific targets in the GPI pathway of pathogens is concerned. Many of the GPI structures and their anchored proteins in pathogenic protozoans and fungi act as pathogenicity factors...
February 27, 2018: Pathogens and Global Health
Mohamed Hanchi, Marie-Christine Thibaud, Bertrand Légeret, Keiko Kuwata, Nathalie Pochon, Fred Beisson, Aiqin Cao, Laura Cuyas, Pascale David, Peter Doerner, Ali Ferjani, Fan Lai, Yonghua Li-Beisson, Jérôme Mutterer, Michel Philibert, Kashchandra G Raghothama, Corinne Rivasseau, David Secco, James Whelan, Laurent Nussaume, Helene Javot
Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis. However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes...
February 23, 2018: Plant Physiology
Hou-Wen Cheng, Hsiao-Wen Wang, Tsung-Yun Wong, Hsien-Wei Yeh, Yi-Chun Chen, Der-Zen Liu, Pi-Hui Liang
S-NeuAc-α(2-6)-di-LacNAc (5) was efficiently synthesized by a [2+2] followed by a [1+4] glycosylation, and later conjugated with 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE) to form both single-layer and multi-layer homogeneous liposomes in the presence of dipalmitoyl phosphatidylcholine (DPPC) and cholesterol. These liposomes were found to be weak inhibitors in both the influenza virus entry assay and the hemagglutination inhibition assay. The single layer liposome was found to more efficiently interfere with the entry of the H1N1 influenza virus into MDCK cells than the multilayer liposome containing 5...
February 12, 2018: Bioorganic & Medicinal Chemistry
Emma K Eriksson, Víctor Agmo Hernández, Katarina Edwards
Ubiquinone-10 (Q10) plays a pivotal role as electron-carrier in the mitochondrial respiratory chain, and is also well known for its powerful antioxidant properties. Recent findings suggest moreover that Q10 could have an important membrane stabilizing function. In line with this, we showed in a previous study that Q10 decreases the permeability to carboxyfluorescein (CF) and increases the mechanical strength of 1-palmitoyl-2-oleyl-sn-glycero-phosphocholine (POPC) membranes. In the current study we report on the effects exerted by Q10 in membranes having a more complex lipid composition designed to mimic that of the inner mitochondrial membrane (IMM)...
February 19, 2018: Biochimica et Biophysica Acta
Jingwen Huang, Xiangyu Zhang, Zhihao Wu, Yufeng Wu, Xiaojie Wu, Yunguang Wang, Hao Jiang, Jing Ma, Zelai He
(Arginine-Glycine-Aspartic)-methoxy polyethylene glycol-(1,2-distearoyl-sn-glycero-3-phosphoethanolaMine-N) (abbreviation: RGD-PEG2000-DSPE or RGD-PD) was successfully synthesized and verified by 1H-NMR and MALDI-TOF MS. Polyethylene glycol-poly-L-lysine/RGD-PD/phospholipid/calcium phosphate nanoparticles (PEG-PLL/RGD-PD/PL/CaP NPs or MNPs) were prepared using a novel, simple method conducted at room temperature. Transmission electron microscopy (TEM) analysis showed that the MNPs were spheres of uniform size, with a diameter of ∼30 nm, and smooth surface...
January 1, 2018: Journal of Biomedical Nanotechnology
Zheng Huang, Dong-Mei Zhao, Xuan Deng, Ji Zhang, Yi-Mei Zhang, Xiao-Qi Yu
The studies of bolaamphiphile-based nanoparticles as delivery vectors are still rudimentary and under development. In this study, several asymmetric bolaamphiphiles containing lysine and another moiety with special functions, such as pH-sensitive or cell-targeting property, were designed and synthesized. The potentials of these bolaamphiphile-based nanoparticles as versatile vectors for both nucleic acids and chemical drugs were studied. With the presence of 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), these amphiphiles could be prepared into bolasomes, which showed good DNA binding ability and could condense plasmid DNA into nanoparticles with appropriate size and surface potential...
February 17, 2018: Nanomaterials
Yongchang Xu, Jingxia Lin, Tao Cui, Swaminath Srinivas, Youjun Feng
Polymyxins such as colistin are antibiotics used as a final line of defense in the management of infections with multidrug-resistant Gram-negative bacteria. Although natural resistance to polymyxins is rare, the discovery of a mobilized colistin resistance gene ( mcr-1 ) in gut bacteria has raised significant concern. As an intramembrane enzyme, MCR-1 catalyzes the transfer of phosphoethanolamine (PEA) to the 1 (or 4')-phosphate group of the lipid A moiety of lipopolysaccharide (LPS), thereby conferring colistin resistance...
February 9, 2018: Journal of Biological Chemistry
Juan M Vanegas, Frank Heinrich, David M Rogers, Bryan D Carson, Sadie La Bauve, Briana C Vernon, Bulent Akgun, Sushil Satija, Aihua Zheng, Margaret Kielian, Susan B Rempe, Michael S Kent
The envelope (E) protein of Dengue virus rearranges to a trimeric hairpin to mediate fusion of the viral and target membranes, which is essential for infectivity. Insertion of E into the target membrane serves to anchor E and possibly also to disrupt local order within the membrane. Both aspects are likely to be affected by the depth of insertion, orientation of the trimer with respect to the membrane normal, and the interactions that form between trimer and membrane. In the present work, we resolved the depth of insertion, the tilt angle, and the fundamental interactions for the soluble portion of Dengue E trimers (sE) associated with planar lipid bilayer membranes of various combinations of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-glycerol (POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), and cholesterol (CHOL) by neutron reflectivity (NR) and by molecular dynamics (MD) simulations...
February 12, 2018: Biochimica et Biophysica Acta
Arthur Cássio de Lima Luna, José Roberto de Assis Santos Filho, Henrique Hesse, Salvador Claro Neto, Gilberto Orivaldo Chierice, Durvanei Augusto Maria
OBJECTIVE: We aimed to evaluate the potential of DODAC/PHO-S liposomes on the modulation of the expression of pro-apoptotic proteins, loss of lysosomal integrity and the mitochondrial electrical potential, compared with phosphoethanolamine. RESULTS: The results of this study demonstrate that DODAC/PHO-S liposomes have exhibited broad cytotoxic potential in B16F10 murine melanoma cells, with significantly greater proportions than treatment with PHO-S. The treatment with the DODAC/PHO-S 2...
February 14, 2018: BMC Research Notes
C Fonta, J-P Salles
The pathophysiology of the neuromuscular manifestations of hypophosphatasia (HPP) remains unknown. Pyridoxine-sensitive seizures characterize severe forms of infantile HPP. Young children and infants affected with severe forms of HPP, but also adults often present with myopathy characterized by hypotonia or muscle weakness. Chronic pain, of unclear mechanism is also often present. Tissue-non-specific alkaline phosphatase (Alkaline Phosphatase-Liver/Bone/Kidney [ALPL]) is expressed in brain neuronal cell and in muscle cells during development and adulthood...
May 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
Liujiang Song, Xiaojun Tao, Li Lin, Chao Chen, Hui Yao, Guangchun He, Guangyang Zou, Zhong Cao, Shichao Yan, Lu Lu, Huimei Yi, Di Wu, Siyuan Tan, Wanxin Ouyang, Zhifei Dai, Xiyun Deng
Triple-negative breast cancer (TNBC) is a subtype of breast cancer with a higher risk in younger women and a poorer prognosis and without targeted therapies available currently. Cancer stem cells (CSCs) are increasingly recognized as the main cause of treatment failure and tumor recurrence. The present paper reported the encapsulation of lovastatin (LV) into cerasomes incorporating distearoyl phosphatidylcholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N -polyethylene glycol 2000 (DSPE-PEG2000) through thin film hydration method in combination with sol-gel reaction and self-assembly process...
February 6, 2018: ACS Applied Materials & Interfaces
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