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brucella canis

Jing Qian, Zhaoyang Bu, Xulong Lang, Guangmou Yan, Yanling Yang, Xiuran Wang, Xinglong Wang
Canine brucellosis, caused by Brucella canis, is a persistent infectious reproductive disease in dogs. The absence of effective treatment to the intracellular pathogen and the irreversible consequence of infection makes the need of a specific vaccine urgent. Bacterial ghosts (BGs) are the empty envelopes of bacteria with no genome content inside, which emerge as a proper vaccine candidate due to its intact outer antigen. It is generally derived from a genetically engineered strain, through the expression of Bacteriophage phiX174 lysis E gene upon induction...
May 2017: Veterinary Microbiology
Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li
BACKGROUND: Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. RESULTS: A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively...
May 15, 2017: BMC Microbiology
Guo-Zhong Tian, Bu-Yun Cui, Dong-Ri Piao, Hong-Yan Zhao, Lan-Yu Li, Xi Liu, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Hai Jiang, Zhen-Jun Li
BACKGROUND: Brucellosis was a common human and livestock disease caused by Brucella strains, the category B priority pathogens by the US Center for Disease Control (CDC). Identified as a priority disease in human and livestock populations, the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking. METHODS: A total of 600 Brucella isolates collected during 60 years (from 1953 to 2013) in China were genotyped by multiple locus variable-number tandem repeat analysis (MLVA) and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index (HGDI) values...
May 2, 2017: Infectious Diseases of Poverty
Yi Yang, Yin Wang, Elizabeth Poulsen, Russell Ransburgh, Xuming Liu, Baoyan An, Nanyan Lu, Gary Anderson, Chengming Wang, Jianfa Bai
Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B...
April 21, 2017: Scientific Reports
(no author information available yet)
No abstract text is available yet for this article.
April 15, 2017: Veterinary Record
Tanya J Purvis, Donna Krouse, Dawn Miller, Julia Livengood, Nagaraja R Thirumalapura, Deepanker Tewari
Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
L B Keid, D P Chiebao, M C A Batinga, T Faita, J A Diniz, T M F de S Oliveira, H L Ferreira, R M Soares
Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. This study aimed to investigate the occurrence of B. canis infection in dogs of commercial breeding kennels located in São Paulo State, Brazil. A total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis through blood culture...
March 13, 2017: Transboundary and Emerging Diseases
Erika Abe, Kazue Ohishi, Tsuyoshi Ishinazaka, Kei Fujii, Tadashi Maruyama
Brucella infection in Hokkaido was serologically surveyed in four species of pinnipeds inhabiting Cape Erimo during 2008-2013 and the Shiretoko Peninsula in 1999 by ELISA using Brucella abortus and B. canis as antigens. Anti-Brucella positive sera showed higher absorbance to B. abortus than B. canis in almost all samples. Anti-B. abortus antibodies were detected in serum samples from 24% (n = 55) of Western Pacific harbor seals (Phoca vitulina stejnegeri) in Cape Erimo and from 66% (n = 41) of spotted seals (P...
April 2017: Microbiology and Immunology
Maria Clausse, Alejandra G Díaz, Romina P Pardo, Vanesa Zylberman, Fernando A Goldbaum, Silvia M Estein
Polymeric antigen BLSOmp31 is an immunogenic vaccine candidate that confers protection against Brucella canis in mice. In this preliminary study, the immunogenicity and safety of BLSOmp31 adsorbed to aluminum hydroxide gel (BLSOmp31-AH) were evaluated in Beagle dogs. In addition, the potential to elicit serum antibodies with complement-dependent bactericidal activity and/or to enhance phagocytosis by neutrophils were analyzed. Dogs were immunized three times with BLSOmp31-AH by subcutaneous route, followed by an annual booster...
February 2017: Veterinary Immunology and Immunopathology
Isis Indaiara Gonçalves Granjeiro Taques, Tatiane Rodrigues Barbosa, Andresa de Cássia Martini, Letícia Camara Pitchenin, Ísis Assis Braga, Andréia Lima Tomé de Melo, Luciano Nakazato, Valéria Dutra, Daniel Moura de Aguiar
Given the fact that numerous microbial species can be detected in pregnant female dogs, the objective of this study was to assess the transplacental transmission of Brucella canis, Ehrlichia canis, Neospora caninum and Toxoplasma gondii in stillborn puppies. This study involved 41 stillborn puppies, 78.6% of which were positive for T. gondii, 52.4% for N. caninum and 59.5% for B. canis. E. canis was not detected in any of the analyzed puppies. Pregnancy is an important physiological condition for the transmission of infectious agents to puppies and transplacental transmission may be epidemiologically relevant in the spread of these opportunistic agents...
December 2016: Comparative Immunology, Microbiology and Infectious Diseases
Serap Ural, Behiye Özer, Fazıl Gelal, Derya Dirim Erdoğan, Nurbanu Sezak, Recep Balık, Tuna Demirdal, Metin Korkmaz
Toxocariasis caused by Toxocara canis or less frequently by T.catis is a common parasitic infection worldwide. Clinical spectrum in humans can vary from asymptomatic infection to serious organ disfunction depending on the load of parasite, migration target of the larva and the inflammatory response of the host. Transverse myelitis (TM) due to toxocariasis is an uncommon illness identified mainly as case reports in literature. In this report, a case of TM who was diagnosed as neurotoxocariasis by serological findings has been presented...
July 2016: Mikrobiyoloji Bülteni
Lauren L Keleher, Jerod A Skyberg
Brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus Brucella. While many species of Brucella exist, Brucella melitensis, Brucella abortus, and Brucella suis are the most common pathogens of humans and livestock. The virulence of Brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. The aim of this study was to characterize the bovine neutrophil response to virulent Brucella spp...
September 2016: Veterinary Immunology and Immunopathology
Hiroshi Ikeda, Keisuke Nagamine
We herein report on a 62-year-old man who presented with symptoms of intermittent fever that persisted after returning from a trip to France. During his trip, he had eaten natural cheese. Although no bacteria could be isolated from blood culture, the serum agglutination test showed a positive antibody titer of 1 : 160 for Brucella canis. The patient responded well to combination antibiotic therapy consisting of gentamicin, rifampicin, and doxycycline, and his symptoms improved. He became antibody-negative after antibiotic therapy...
March 2016: Kansenshōgaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
Murat Kutlu, Nural Cevahir, Sevil Erdenliğ-Gürbilek, Şerife Akalın, Mehmet Uçar, Selda Sayın-Kutlu
BACKGROUND: Brucella melitensis and B. abortus are the species generally isolated from human samples in Turkey. Several studies have also demonstrated the presence of antibodies against B. canis. CASE REPORT AND STUDY: Brucella spp. was isolated from blood culture from a 35-year-old male with clinical signs and symptoms of acute meningitis, including fever lasting for 1 week. Multiplex PCR demonstrated B. suis, and biochemical features indicated biovar 1. CONCLUSIONS: This report is the first emphasizing that B...
September 2016: Journal of Infection and Public Health
Lucía Paula Alvarez, Guillermo García-Effrón, Carlos Alejandro Robles
Brucellosis caused by Brucella ovis is one of the most important infectious diseases of sheep. The aim of this study was to determine the presence of genes both inside and outside the specific B. ovis pathogenicity island 1 (BOPI-1) in a large collection of field isolates of B. ovis and other Brucella spp. from Argentina. The BOV_A0500 gene from B. ovis BOPI-1 was identified in all 104 B. ovis isolates studied. The BOPI-1 complete sequence was found to be conserved in 10 B. ovis strains from the collection, for which whole genome sequencing was performed...
March 2016: Veterinary Journal
A Peña, K Abarca, T Weitzel, J Gallegos, J Cerda, P García, J López
Although pets provide physiological and psychological benefits to their owners, they are a potential source of zoonotic infections, especially for vulnerable individuals such as immunocompromised patients. During 1 year, we therefore performed a pilot project, which included 32 immunocompromised Chilean children and their family pets (35 dogs and 9 cats) with the aim of detecting, treating and preventing zoonotic infections. Children were examined by Infectious Diseases paediatricians and demographical and clinical information related to zoonotic infections were recorded...
August 2016: Zoonoses and Public Health
Mariana Malzoni Furtado, Solange Maria Gennari, Cassia Yumi Ikuta, Anah Tereza de Almeida Jácomo, Zenaide Maria de Morais, Hilda Fátima de Jesus Pena, Grasiela Edith de Oliveira Porfírio, Leandro Silveira, Rahel Sollmann, Gisele Oliveira de Souza, Natália Mundim Tôrres, José Soares Ferreira Neto
This study investigated the exposure of jaguar populations and domestic animals to smooth Brucella, Leptospira spp. and Toxoplasma gondii in the Cerrado, Pantanal and Amazon biomes of Brazil. Between February 2000 and January 2010, serum samples from 31 jaguars (Panthera onca), 1,245 cattle (Bos taurus), 168 domestic dogs (Canis lupus familiaris) and 29 domestic cats (Felis catus) were collected and analysed by rose bengal test for smooth Brucella, microscopic agglutination test for Leptospira spp. and modified agglutination test for T...
2015: PloS One
Afshar Etemady, Mohsen Mohammdi, Majid Esmaelizad, Saeed Alamian, Fatemeh Vahedi, Khosro Aghaeipour, Ali Mohammad Behrozikhah, Ebrahim Faghihloo, Davoud Afshar, Sajad Firuzyar, Arian Rahimi
INTRODUCTION: Brucella spp. are gram-negative, facultative intracellular bacteria pathogens responsible for brucellosis, a zoonotic disease that can cause abortion, fetal death, and genital infections in animals and undulant fever in humans. Lipopolysaccharide (LPS) is known as a major virulence factor of Brucella spp. The wboA gene is capable of encoding a glycosyltransferase that appears to play a major role in LPS biosynthesis. Hence, the characterization of this gene can help in the clarification of the pathogenicity of Brucella spp...
October 2015: Electronic Physician
L B Keid, J A Diniz, T M F S Oliveira, H L Ferreira, R M Soares
This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2-mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups: B...
December 2015: Reproduction in Domestic Animals, Zuchthygiene
Sanjay Kapil
A large breeding kennel of Bulldogs (n = 57) experienced several Canid herpesvirus 1 (CHV-1)-related diseases in older puppies (9 weeks of age) in Arkansas. CHV-1 has been repeatedly confirmed in the kennel in several animals for 3 years (January 2012-February 2015) using various virology tests. I was able to detect a partial sequence of CHV DNA (~120 bp) in archived formalin-fixed, paraffin-embedded tissue blocks after 3 years of storage. CHV-1 is persistently circulating in this kennel in spite of high serum antibody titers in the adult dogs...
November 2015: Journal of Veterinary Diagnostic Investigation
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