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brucella canis

Serap Ural, Behiye Özer, Fazıl Gelal, Derya Dirim Erdoğan, Nurbanu Sezak, Recep Balık, Tuna Demirdal, Metin Korkmaz
Toxocariasis caused by Toxocara canis or less frequently by T.catis is a common parasitic infection worldwide. Clinical spectrum in humans can vary from asymptomatic infection to serious organ disfunction depending on the load of parasite, migration target of the larva and the inflammatory response of the host. Transverse myelitis (TM) due to toxocariasis is an uncommon illness identified mainly as case reports in literature. In this report, a case of TM who was diagnosed as neurotoxocariasis by serological findings has been presented...
July 2016: Mikrobiyoloji Bülteni
Lauren L Keleher, Jerod A Skyberg
Brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus Brucella. While many species of Brucella exist, Brucella melitensis, Brucella abortus, and Brucella suis are the most common pathogens of humans and livestock. The virulence of Brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. The aim of this study was to characterize the bovine neutrophil response to virulent Brucella spp...
September 2016: Veterinary Immunology and Immunopathology
Hiroshi Ikeda, Keisuke Nagamine
We herein report on a 62-year-old man who presented with symptoms of intermittent fever that persisted after returning from a trip to France. During his trip, he had eaten natural cheese. Although no bacteria could be isolated from blood culture, the serum agglutination test showed a positive antibody titer of 1 : 160 for Brucella canis. The patient responded well to combination antibiotic therapy consisting of gentamicin, rifampicin, and doxycycline, and his symptoms improved. He became antibody-negative after antibiotic therapy...
March 2016: Kansenshōgaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
Murat Kutlu, Nural Cevahir, Sevil Erdenliğ-Gürbilek, Şerife Akalın, Mehmet Uçar, Selda Sayın-Kutlu
BACKGROUND: Brucella melitensis and B. abortus are the species generally isolated from human samples in Turkey. Several studies have also demonstrated the presence of antibodies against B. canis. CASE REPORT AND STUDY: Brucella spp. was isolated from blood culture from a 35-year-old male with clinical signs and symptoms of acute meningitis, including fever lasting for 1 week. Multiplex PCR demonstrated B. suis, and biochemical features indicated biovar 1. CONCLUSIONS: This report is the first emphasizing that B...
September 2016: Journal of Infection and Public Health
Lucía Paula Alvarez, Guillermo García-Effrón, Carlos Alejandro Robles
Brucellosis caused by Brucella ovis is one of the most important infectious diseases of sheep. The aim of this study was to determine the presence of genes both inside and outside the specific B. ovis pathogenicity island 1 (BOPI-1) in a large collection of field isolates of B. ovis and other Brucella spp. from Argentina. The BOV_A0500 gene from B. ovis BOPI-1 was identified in all 104 B. ovis isolates studied. The BOPI-1 complete sequence was found to be conserved in 10 B. ovis strains from the collection, for which whole genome sequencing was performed...
March 2016: Veterinary Journal
A Peña, K Abarca, T Weitzel, J Gallegos, J Cerda, P García, J López
Although pets provide physiological and psychological benefits to their owners, they are a potential source of zoonotic infections, especially for vulnerable individuals such as immunocompromised patients. During 1 year, we therefore performed a pilot project, which included 32 immunocompromised Chilean children and their family pets (35 dogs and 9 cats) with the aim of detecting, treating and preventing zoonotic infections. Children were examined by Infectious Diseases paediatricians and demographical and clinical information related to zoonotic infections were recorded...
August 2016: Zoonoses and Public Health
Mariana Malzoni Furtado, Solange Maria Gennari, Cassia Yumi Ikuta, Anah Tereza de Almeida Jácomo, Zenaide Maria de Morais, Hilda Fátima de Jesus Pena, Grasiela Edith de Oliveira Porfírio, Leandro Silveira, Rahel Sollmann, Gisele Oliveira de Souza, Natália Mundim Tôrres, José Soares Ferreira Neto
This study investigated the exposure of jaguar populations and domestic animals to smooth Brucella, Leptospira spp. and Toxoplasma gondii in the Cerrado, Pantanal and Amazon biomes of Brazil. Between February 2000 and January 2010, serum samples from 31 jaguars (Panthera onca), 1,245 cattle (Bos taurus), 168 domestic dogs (Canis lupus familiaris) and 29 domestic cats (Felis catus) were collected and analysed by rose bengal test for smooth Brucella, microscopic agglutination test for Leptospira spp. and modified agglutination test for T...
2015: PloS One
Afshar Etemady, Mohsen Mohammdi, Majid Esmaelizad, Saeed Alamian, Fatemeh Vahedi, Khosro Aghaeipour, Ali Mohammad Behrozikhah, Ebrahim Faghihloo, Davoud Afshar, Sajad Firuzyar, Arian Rahimi
INTRODUCTION: Brucella spp. are gram-negative, facultative intracellular bacteria pathogens responsible for brucellosis, a zoonotic disease that can cause abortion, fetal death, and genital infections in animals and undulant fever in humans. Lipopolysaccharide (LPS) is known as a major virulence factor of Brucella spp. The wboA gene is capable of encoding a glycosyltransferase that appears to play a major role in LPS biosynthesis. Hence, the characterization of this gene can help in the clarification of the pathogenicity of Brucella spp...
October 2015: Electronic Physician
L B Keid, J A Diniz, T M F S Oliveira, H L Ferreira, R M Soares
This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2-mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups: B...
December 2015: Reproduction in Domestic Animals, Zuchthygiene
Sanjay Kapil
A large breeding kennel of Bulldogs (n = 57) experienced several Canid herpesvirus 1 (CHV-1)-related diseases in older puppies (9 weeks of age) in Arkansas. CHV-1 has been repeatedly confirmed in the kennel in several animals for 3 years (January 2012-February 2015) using various virology tests. I was able to detect a partial sequence of CHV DNA (~120 bp) in archived formalin-fixed, paraffin-embedded tissue blocks after 3 years of storage. CHV-1 is persistently circulating in this kennel in spite of high serum antibody titers in the adult dogs...
November 2015: Journal of Veterinary Diagnostic Investigation
Carlos Chacón-Díaz, Pamela Altamirano-Silva, Gabriela González-Espinoza, María-Concepción Medina, Alejandro Alfaro-Alarcón, Laura Bouza-Mora, César Jiménez-Rojas, Melissa Wong, Elías Barquero-Calvo, Norman Rojas, Caterina Guzmán-Verri, Edgardo Moreno, Esteban Chaves-Olarte
Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. Studies of B. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. A virulent B. canis strain isolated from those outbreaks readily replicated in different organs of mice for a protracted period...
December 2015: Infection and Immunity
Angelika Silbereisen, Marco Tamborrini, Matthias Wittwer, Nadia Schürch, Gerd Pluschke
BACKGROUND: Brucella, a Gram-negative bacterium, is classified as a potential bioterrorism agent mainly due to the low dose needed to cause infection and the ability to transmit the bacteria via aerosols. Goats/sheep, cattle, pigs, dogs, sheep and rodents are infected by B. melitensis, B. abortus, B. suis, B. canis, B. ovis and B. neotomae, respectively, the six classical Brucella species. Most human cases are caused by B. melitensis and B. abortus. Our aim was to specifically detect Brucellae with 'smooth' lipopolysaccharide (LPS) using a highly sensitive monoclonal antibody (mAb) based immunological assay...
2015: BMC Microbiology
Quang Lam Truong, Youngjae Cho, Kiju Kim, Bo-Kyoung Park, Tae-Wook Hahn
Brucella abortus attenuated strain RB51 vaccine (RB51) is widely used in prevention of bovine brucellosis. Although vaccination with this strain has been shown to be effective in conferring protection against bovine brucellosis, RB51 has several drawbacks, including residual virulence for animals and humans. Therefore, a safe and efficacious vaccine is needed to overcome these disadvantages. In this study, we constructed several gene deletion mutants (ΔcydC, ΔcydD and ΔpurD single mutants, and ΔcydCΔcydD and ΔcydCΔpurD double mutants) of RB51 with the aim of increasing the safety of the possible use of these mutants as vaccine candidates...
November 2015: Microbiology
Zsuzsanna Rónai, Zsuzsa Kreizinger, Ádám Dán, Kevin Drees, Jeffrey T Foster, Krisztián Bányai, Szilvia Marton, Levente Szeredi, Szilárd Jánosi, Miklós Gyuranecz
BACKGROUND: Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary. RESULTS: The B...
2015: BMC Veterinary Research
Consuelo Borie, Nicolás Galarce
No abstract text is available yet for this article.
April 2015: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Peter Lasch, Tara Wahab, Sandra Weil, Bernadett Pályi, Herbert Tomaso, Sabine Zange, Beathe Kiland Granerud, Michal Drevinek, Branko Kokotovic, Matthias Wittwer, Valentin Pflüger, Antonino Di Caro, Maren Stämmler, Roland Grunow, Daniela Jacob
In the case of a release of highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, and genotyping of HPB. In the present study, the results of a joint exercise with 11 partner institutions from nine European countries are presented. In this exercise, 10 distinct microbial samples, among them five HPB, Bacillus anthracis, Brucella canis, Burkholderia mallei, Burkholderia pseudomallei, and Yersinia pestis, were characterized under blinded conditions...
August 2015: Journal of Clinical Microbiology
Gwen Jankowski, Michael J Adkesson, Jeremiah T Saliki, Susana Cárdenas-Alayza, Patricia Majluf
The Peruvian population of the South American fur seal ( Arctocephalus australis ) is a distinct evolutionarily significant unit that is endangered. One of the largest rookeries for this species in Peru is located within the Punta San Juan marine protected area (15°22'S, 75°12'W). To better understand the current health status of this population, exposure to 10 pinniped pathogens was evaluated in adult female fur seals (n=29) via serology and polymerase chain reaction (PCR) techniques in November 2010. The results suggest this population is naïve to canine and phocine distemper viruses (serum neutralization test), five Leptospira interrogans serovars (microscopic agglutination test), and Brucella canis (card test)...
June 2015: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
M Colter Chitwood, Morgan B Swingen, Marcus A Lashley, James R Flowers, Maria B Palamar, Charles S Apperson, Colleen Olfenbuttel, Christopher E Moorman, Christopher S DePerno
Coyotes (Canis latrans) have expanded recently into the eastern US and can serve as a source of pathogens to domestic dogs (Canis lupus familiaris), livestock, and humans. We examined free-ranging coyotes from central North Carolina, US, for selected parasites and prevalence of antibodies against viral and bacterial agents. We detected ticks on most (81%) coyotes, with Amblyomma americanum detected on 83% of those with ticks. Fifteen (47%) coyotes were positive for heartworms (Dirofilaria immitis), with a greater detection rate in adults (75%) than juveniles (22%)...
July 2015: Journal of Wildlife Diseases
Miryan Margot Sánchez-Jiménez, Juan Pablo Isaza, Juan F Alzate, Martha Olivera-Angel
Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years. In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing-CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2...
July 2015: Genomics
Jama'ayah Mohamed Zahidi, Tay Bee Yong, Rohaidah Hashim, Azura Mohd Noor, Siti Hawa Hamzah, Norazah Ahmad
Molecular approaches have been investigated to overcome difficulties in identification and differentiation of Brucella spp. using conventional phenotypic methods. In this study, high-resolution melt (HRM) analysis was used for rapid identification and differentiation of members of Brucella genus. A total of 41 Brucella spp. isolates from human brucellosis were subjected to HRM analysis using 4 sets of primers, which identified 40 isolates as Brucella melitensis and 1 as Brucella canis. The technique utilized low DNA concentration and was highly reproducible...
April 2015: Diagnostic Microbiology and Infectious Disease
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