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brucella canis

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https://www.readbyqxmd.com/read/29524641/comparative-application-of-is711-based-polymerase-chain-reaction-pcr-and-loop-mediated-isothermal-amplification-lamp-for-canine-brucellosis-diagnosis
#1
Maria Cryskely Agra Batinga, Julia Teresa Ribeiro de Lima, Fabio Gregori, Jaqueline Assumpção Diniz, Kerstin Muner, Trícia M F S Oliveira, Helena Lage Ferreira, Rodrigo Martins Soares, Lara Borges Keid
Canine brucellosis is caused by Brucella canis, a gram negative and facultative intracellular bacterium that is commonly associated with reproductive failures in dogs. The accurate diagnosis of the infection relies on the use of serological tests associated with blood culturing to guarantee sensitivity. The polymerase chain reaction (PCR) can replace the culturing procedure for the direct diagnosis of the infection because of its speed, high specificity and sensitivity values; however, it depends on some laboratory infrastructure to be conducted...
March 7, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29491236/human-infection-with-anaplasma-phagocytophilum-in-inner-mongolia-china
#2
Gaowa, Wulantuya, Xuhong Yin, Minzhi Cao, Shengchun Guo, Chunlian Ding, Lu Yuhua, Luo Jianchang, Hiroki Kawabata, Shuji Ando, Hongru Su, Masahiko Shimada, Naoya Takamoto, Yuko Shimamura, Shuichi Masuda, Norio Ohashi
Anaplasma phagocytophilum is an obligate intracellular bacterium and causes febrile illness in humans and livestock. A 49-year-old woman was suffering from feverish symptom, fatigue, arthralgia, general body pain, and anorexia for 2 weeks. Later, she visited Bayan Nur Centers for Disease Control and Prevention Hospital in Inner Mongolia, China. Molecular-based diagnostic analysis of the patient's blood revealed that A. phagocytophilum p44 DNA was positive, but Brucella omp31, spotted fever group Rickettsia gltA, Orientia tsutsugamushi 16S rDNA, and Ehrlichia p28 were negative...
February 28, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29361960/a-rapid-minor-groove-binder-pcr-method-for-distinguishing-the-vaccine-strain-brucella-abortus-104m
#3
Wenlong Nan, Lide Qin, Yong Wang, Yueyong Zhang, Pengfei Tan, Yuqi Chen, Kairong Mao, Yiping Chen
BACKGROUND: Brucellosis is a widespread zoonotic disease caused by Gram-negative Brucella bacteria. Immunisation with attenuated vaccine is an effective method of prevention, but it can interfere with diagnosis. Live, attenuated Brucella abortus strain 104M has been used for the prevention of human brucellosis in China since 1965. However, at present, no fast and reliable method exists that can distinguish this strain from field strains. Single nucleotide polymorphism (SNP)-based assays offer a new approach for such discrimination...
January 24, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29302106/-brucella-canis-an-update-on-research-and-clinical-management
#4
REVIEW
Kevin L Cosford
In Canada, Brucella canis remains a potentially devastating infectious agent that is still considered uncommon, despite the increasing international movement of dogs. There may be a growing risk to the Canadian canine population due to a reliance on outdated seroprevalence studies and the lack of federal regulation. With the complex diagnostic and management challenges associated with Brucella canis, a One Health approach is necessary to address the need for ongoing research, including updating canine and human seroprevalence rates in Canada, elucidating the pathogenesis, and determining the most appropriate treatment and prevention strategies...
January 2018: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/29277251/-characterization-of-the-genetic-variability-of-field-strains-of-brucella-canis-isolated-in-antioquia
#5
Juana L Vidal Arboleda, Luisa F Ortiz Roman, Martha Olivera Angel
Brucella canis is a facultative intracellular pathogen responsible for canine brucellosis, a zoonotic disease that affects canines, causing abortions and reproductive failure; and the production of non-specific symptoms in humans. In 2005 the presence of B. canis in Antioquia was demonstrated and the strains were identified as type 2. The sequencing of the genome of a field strain denoted Brucella canis str. Oliveri, showed species-specific indel events, which led us to investigate the genomic characteristics of the B...
December 22, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29173617/identification-of-brucella-genus-and-eight-brucella-species-by-luminex-bead-based-suspension-array
#6
Tina S Lusk Pfefer, Ruth Timme, Julie A Kase
Globally, unpasteurized milk products are vehicles for the transmission of brucellosis, a zoonosis responsible for cases of foodborne illness in the United States and elsewhere. Existing PCR assays to detect Brucella species are restricted by the resolution of band sizes on a gel or the number of fluorescent channels in a single real-time system. The Luminex bead-based suspension array is performed in a 96-well plate allowing for high throughput screening of up to 100 targets in one sample with easily discernible results...
April 2018: Food Microbiology
https://www.readbyqxmd.com/read/29096717/variability-in-the-response-of-canine-and-human-dendritic-cells-stimulated-with-brucella-canis
#7
Myriam Pujol, Francisca Castillo, Carla Alvarez, Camila Rojas, Consuelo Borie, Arturo Ferreira, Rolando Vernal
Brucella canis is a small intracellular Gram-negative bacterium whose primary host is the dog, but it also can cause mild human brucellosis. One of the main causes of an inefficient immune response against other species of Brucella is their interaction with dendritic cells (DCs), which affects antigen presentation and impairs the development of an effective Th1 immune response. This study analysed the cytokine pattern production, by RT-qPCR and ELISA, in human and canine DCs against whole B. canis or its purified LPS...
November 2, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29036184/proinflammatory-response-of-canine-trophoblasts-to-brucella-canis-infection
#8
Andrea G Fernández, M Soledad Hielpos, Mariana C Ferrero, Carlos A Fossati, Pablo C Baldi
Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis-induced abortion are unknown, but heavily infected trophoblasts are consistently observed. As trophoblasts responses to other pathogens contribute to placental inflammation leading to abortion, the aim of the present study was to characterize the cytokine response of canine trophoblasts to B. canis infection. To achieve this, trophoblasts isolated from term placenta of healthy female dogs were infected with B...
2017: PloS One
https://www.readbyqxmd.com/read/29021156/safety-and-accuracy-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-highly-pathogenic-organisms
#9
James T Rudrik, Marty K Soehnlen, Michael J Perry, Maureen M Sullivan, Wanda Reiter-Kintz, Philip A Lee, Denise Pettit, Anthony Tran, Erin Swaney
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-plate formic acid, and ethanol/formic acid tube extraction methods, were evaluated for their ability to render highly pathogenic organisms nonviable and safe for handling in a biosafety level 2 laboratory. Of these, the tube extraction procedure was the most successful, with none of the tested strains surviving this sample preparation method. Tube extracts from several agents of bioterrorism and their near neighbors were analyzed in an eight-laboratory study to examine the utility of the Bruker Biotyper and Vitek MS MALDI-TOF MS systems and their in vitro diagnostic (IVD), research-use-only, and Security-Relevant databases, as applicable, to accurately identify these agents...
December 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28964545/diagnostic-imaging-of-discospondylitis
#10
REVIEW
Catherine M Ruoff, Sharon C Kerwin, Amanda R Taylor
Discospondylitis can affect dogs of any age and breed and may be seen in cats. Although radiography remains the gold standard, advanced imaging, such as CT and MRI, has benefits and likely allows earlier diagnosis and identification of concurrent disease. Because discospondylitis may affect multiple disk spaces, imaging of the entire spine should be considered. There is a lengthening list of causative etiologic agents, and successful treatment hinges on correct identification. Image-guided biopsy should be considered in addition to blood and urine cultures and Brucella canis screening and as an alternative to surgical biopsy in some cases...
January 2018: Veterinary Clinics of North America. Small Animal Practice
https://www.readbyqxmd.com/read/28888634/development-of-improved-enzyme-based-and-lateral-flow-immunoassays-for-rapid-and-accurate-serodiagnosis-of-canine-brucellosis
#11
María E Cortina, Analía Novak, Luciano J Melli, Sebastián Elena, Natalia Corbera, Juan E Romero, Ana M Nicola, Juan E Ugalde, Diego J Comerci, Andrés E Ciocchini
Brucellosis is a widespread zoonotic disease caused by Brucella spp. Brucella canis is the etiological agent of canine brucellosis, a disease that can lead to sterility in bitches and dogs causing important economic losses in breeding kennels. Early and accurate diagnosis of canine brucellosis is central to control the disease and lower the risk of transmission to humans. Here, we develop and validate enzyme and lateral flow immunoassays for improved serodiagnosis of canine brucellosis using as antigen the B...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28864247/comparison-of-three-methods-for-recovery-of-brucella-canis-dna-from-canine-blood-samples
#12
Maria Cryskely A Batinga, Jaíne C Dos Santos, Julia T R Lima, Maria Fernanda D Bigotto, Kerstin Muner, Thalita Faita, Rodrigo M Soares, David A V da Silva, Trícia M F S Oliveira, Helena L Ferreira, Jaqueline A Diniz, Lara B Keid
Brucella canis, a gram-negative, facultative intracellular and zoonotic bacterium causes canine brucellosis. Direct methods are the most appropriate for the detection of canine brucellosis and bacterial isolation from blood samples has been employed as gold-standard method. However, due to the delay in obtaining results and the biological risk of the bacterial culturing, the polymerase chain reaction (PCR) has been successfully used as an alternative method for the diagnosis of the infection. Sample preparation is a key step for successful PCR and protocols that provide high DNA yield and purity are recommended to ensure high diagnostic sensitivity...
August 31, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28532790/a-safe-and-molecular-tagged-brucella-canis-ghosts-confers-protection-against-virulent-challenge-in-mice
#13
Jing Qian, Zhaoyang Bu, Xulong Lang, Guangmou Yan, Yanling Yang, Xiuran Wang, Xinglong Wang
Canine brucellosis, caused by Brucella canis, is a persistent infectious reproductive disease in dogs. The absence of effective treatment to the intracellular pathogen and the irreversible consequence of infection makes the need of a specific vaccine urgent. Bacterial ghosts (BGs) are the empty envelopes of bacteria with no genome content inside, which emerge as a proper vaccine candidate due to its intact outer antigen. It is generally derived from a genetically engineered strain, through the expression of Bacteriophage phiX174 lysis E gene upon induction...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28506316/characterization-of-novel-omp31-antigenic-epitopes-of-brucella-melitensis-by-monoclonal-antibodies
#14
Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li
BACKGROUND: Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. RESULTS: A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28460642/multi-locus-variable-number-tandem-repeat-analysis-of-chinese-brucella-strains-isolated-from-1953-to-2013
#15
Guo-Zhong Tian, Bu-Yun Cui, Dong-Ri Piao, Hong-Yan Zhao, Lan-Yu Li, Xi Liu, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Hai Jiang, Zhen-Jun Li
BACKGROUND: Brucellosis was a common human and livestock disease caused by Brucella strains, the category B priority pathogens by the US Center for Disease Control (CDC). Identified as a priority disease in human and livestock populations, the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking. METHODS: A total of 600 Brucella isolates collected during 60 years (from 1953 to 2013) in China were genotyped by multiple locus variable-number tandem repeat analysis (MLVA) and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index (HGDI) values...
May 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28432327/genotyping-brucella-canis-isolates-using-a-highly-discriminatory-multilocus-variable-number-tandem-repeat-analysis-mlva-assay
#16
Yi Yang, Yin Wang, Elizabeth Poulsen, Russell Ransburgh, Xuming Liu, Baoyan An, Nanyan Lu, Gary Anderson, Chengming Wang, Jianfa Bai
Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28408516/-brucella-canis-in-a-dog-in-the-uk
#17
LETTER
(no author information available yet)
No abstract text is available yet for this article.
April 15, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28381135/detection-of-brucella-canis-infection-in-dogs-by-blood-culture-and-bacterial-identification-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#18
Tanya J Purvis, Donna Krouse, Dawn Miller, Julia Livengood, Nagaraja R Thirumalapura, Deepanker Tewari
Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B...
July 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28296215/brucella-canis-infection-in-dogs-from-commercial-breeding-kennels-in-brazil
#19
L B Keid, D P Chiebao, M C A Batinga, T Faita, J A Diniz, T M F de S Oliveira, H L Ferreira, R M Soares
Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. This study aimed to investigate the occurrence of B. canis infection in dogs of commercial breeding kennels located in São Paulo State, Brazil. A total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis through blood culture...
June 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28262966/serologic-evidence-of-brucella-infection-in-pinnipeds-along-the-coast-of-hokkaido-the-northernmost-main-island-of-japan
#20
Erika Abe, Kazue Ohishi, Tsuyoshi Ishinazaka, Kei Fujii, Tadashi Maruyama
Brucella infection in Hokkaido was serologically surveyed in four species of pinnipeds inhabiting Cape Erimo during 2008-2013 and the Shiretoko Peninsula in 1999 by ELISA using Brucella abortus and B. canis as antigens. Anti-Brucella positive sera showed higher absorbance to B. abortus than B. canis in almost all samples. Anti-B. abortus antibodies were detected in serum samples from 24% (n = 55) of Western Pacific harbor seals (Phoca vitulina stejnegeri) in Cape Erimo and from 66% (n = 41) of spotted seals (P...
April 2017: Microbiology and Immunology
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