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https://www.readbyqxmd.com/read/28221358/ionic-solutions-of-two-dimensional-materials
#1
Patrick L Cullen, Kathleen M Cox, Mohammed K Bin Subhan, Loren Picco, Oliver D Payton, David J Buckley, Thomas S Miller, Stephen A Hodge, Neal T Skipper, Vasiliki Tileli, Christopher A Howard
Strategies for forming liquid dispersions of nanomaterials typically focus on retarding reaggregation, for example via surface modification, as opposed to promoting the thermodynamically driven dissolution common for molecule-sized species. Here we demonstrate the true dissolution of a wide range of important 2D nanomaterials by forming layered material salts that spontaneously dissolve in polar solvents yielding ionic solutions. The benign dissolution advantageously maintains the morphology of the starting material, is stable against reaggregation and can achieve solutions containing exclusively individualized monolayers...
March 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28219189/-effects-of-phentolamine-on-haemodynamics-of-patients-with-severe-sepsis
#2
W Yang, X B Li, Y Zhou, X M Mo
Objective: To explore the effects of phentolamine on hemodynamic of patients with severe sepsis . Methods: From January 2015 to August 2016, using random number table, 59 patients with severe sepsis were divided into research group and the control group by conventional treatment in Department of Emergency, the Second People's Hospital of Nanning City. The patients of the research group were given phentolamine injection. The CI, ITBVI, EVLWI, SVRI of the patients were monitored by PICCO on 6, 12, 24, 48, 72 h before and after treatment...
February 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28215339/precise-tracking-of-the-dynamics-of-multiple-proteins-in-endocytic-events
#3
A Picco, M Kaksonen
Endocytosis is a complex and dynamic process that involves dozens of different proteins to define the site of endocytosis, form a membrane invagination, and pinch off a membrane vesicle into the cytoplasm. Fluorescent light microscopy is a powerful tool to visualize the dynamic behaviors of the proteins taking part in the endocytic process. The resolution of light microscopy is, however, a serious limitation. Here, we detail a fluorescence microscope method that we have developed to visualize the dynamics of the clathrin-mediated endocytic protein machinery in yeast cells...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28208323/upper-and-lower-critical-decay-exponents-of-ising-ferromagnets-with-long-range-interaction
#4
Toshiki Horita, Hidemaro Suwa, Synge Todo
We investigate the universality class of the finite-temperature phase transition of the two-dimensional Ising model with the algebraically decaying ferromagnetic long-range interaction, J_{ij}=|r[over ⃗]_{i}-r[over ⃗]_{j}|^{-(d+σ)}, where d (=2) is the dimension of the system and σ is the decay exponent, by means of the order-N cluster-algorithm Monte Carlo method. In particular, we focus on the upper and lower critical decay exponents, the boundaries between the mean-field-universality, intermediate, and short-range-universality regimes...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28205080/assessment-of-cu-zn-edta-parenteral-toxicity-in-calves
#5
Luis Emilio Fazzio, Diana Esther Rosa, Sebastian Julio Picco, Guillermo Alberto Mattioli
Copper (Cu) parenteral administration is used in a beef cow-calf operations to prevent or correct Cu deficiency in bovines. At present, Zinc (Zn) salts have been incorporated to complement Cu antioxidant effect. A risk of hepatotoxicity generated by overdose is a negative consequence of injectable Cu application. Cu-Zn EDTA appears as an alternative; however, data about its toxicity is unknown. The aim of this study was to assess toxicity risk of different doses of Cu-Zn EDTA in calves. Thirty two Aberdeen Angus calves of 162 (±20) kg BW were assigned to 4 groups (n = 8), homogeneous in weight, sex, and age...
February 15, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28188408/cross-comparisons-of-trending-accuracies-of-continuous-cardiac-output-measurements-pulse-contour-analysis-bioreactance-and-pulmonary-artery-catheter
#6
Bouchra Lamia, Hyung Kook Kim, Donald A Severyn, Michael R Pinsky
We compared the similarity of cardiac-output (CO) estimates between available bolus thermodilution pulmonary-artery catheters (PAC), arterial pulse-contour analysis (LiDCOplus(™), FloTrac(™) and PiCCOplus(™)), and bioreactance (NICOM(™)). Repetitive simultaneous estimates of CO obtained from the above devices were compared in 21 cardiac-surgery patients during the first 2 h post-surgery. Mean and absolute values for CO across the devices were compared by ANOVA, Bland-Altman, Pearson moment, and linear-regression analyses...
February 10, 2017: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/28162781/intra-articular-corticosteroids-versus-intra-articular-corticosteroids-plus-methotrexate-in-oligoarticular-juvenile-idiopathic-arthritis-a-multicentre-prospective-randomised-open-label-trial
#7
Angelo Ravelli, Sergio Davì, Giulia Bracciolini, Angela Pistorio, Alessandro Consolaro, Evert Hendrik Pieter van Dijkhuizen, Bianca Lattanzi, Giovanni Filocamo, Sara Verazza, Valeria Gerloni, Maurizio Gattinara, Irene Pontikaki, Antonella Insalaco, Fabrizio De Benedetti, Adele Civino, Giuseppe Presta, Luciana Breda, Valentina Marzetti, Serena Pastore, Silvia Magni-Manzoni, Maria Cristina Maggio, Franco Garofalo, Donato Rigante, Marco Gattorno, Clara Malattia, Paolo Picco, Stefania Viola, Stefano Lanni, Nicolino Ruperto, Alberto Martini
BACKGROUND: Little evidence-based information is available to guide the treatment of oligoarticular juvenile idiopathic arthritis. We aimed to investigate whether oral methotrexate increases the efficacy of intra-articular corticosteroid therapy. METHODS: We did this prospective, open-label, randomised trial at ten hospitals in Italy. Using a concealed computer-generated list, children younger than 18 years with oligoarticular-onset disease were randomly assigned (1:1) to intra-articular corticosteroids alone or in combination with oral methotrexate (15 mg/m(2); maximum 20 mg)...
February 2, 2017: Lancet
https://www.readbyqxmd.com/read/28129539/the-in%C3%A2-vivo-architecture-of-the-exocyst-provides-structural-basis-for-exocytosis
#8
Andrea Picco, Ibai Irastorza-Azcarate, Tanja Specht, Dominik Böke, Irene Pazos, Anne-Sophie Rivier-Cordey, Damien P Devos, Marko Kaksonen, Oriol Gallego
The structural characterization of protein complexes in their native environment is challenging but crucial for understanding the mechanisms that mediate cellular processes. We developed an integrative approach to reconstruct the 3D architecture of protein complexes in vivo. We applied this approach to the exocyst, a hetero-octameric complex of unknown structure that is thought to tether secretory vesicles during exocytosis with a poorly understood mechanism. We engineered yeast cells to anchor the exocyst on defined landmarks and determined the position of its subunit termini at nanometer precision using fluorescence microscopy...
January 26, 2017: Cell
https://www.readbyqxmd.com/read/28113244/stem-cell-plasticity-and-niche-dynamics-in-cancer-progression
#9
Noemi Picco, Robert A Gatenby, Alexander R A Anderson
OBJECTIVE: Cancer stem cells (CSCs) have been hypothesized to initiate and drive tumor growth and recurrence due to their self-renewal ability. If correct, this hypothesis implies that successful therapy must focus primarily on eradication of this CSC fraction. However, recent evidence suggests stemness is niche dependent and may represent one of many phenotypic states that can be accessed by many cancer genotypes when presented with specific environmental cues. A better understanding of the relationship of stemness to niche-related phenotypic plasticity could lead to alternative treatment strategies...
September 8, 2016: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28113098/gender-differences-in-positive-mental-health-among-individuals-with-schizophrenia
#10
Anitha Jeyagurunathan, Janhavi Ajit Vaingankar, Edimansyah Abdin, Rajeswari Sambasivam, Esmond Seow, Shirlene Pang, Louisa Picco, Siow Ann Chong, Mythily Subramaniam
OBJECTIVE: This article aims to investigate the gender differences in positive mental health among outpatients with schizophrenia. METHODS: 142 outpatients (aged 21-65 years) with schizophrenia spectrum disorders were recruited from a tertiary psychiatric hospital. They were administered the following instruments: The Positive Mental Health (PMH) instrument, Patient Health Questionnaire (PHQ)-8 and Generalized Anxiety Disorder (GAD)-7, and the Global Assessment of Functioning (GAF) scale...
January 12, 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/28100566/loss-of-axin1-drives-acquired-resistance-to-wnt-pathway-blockade-in-colorectal-cancer-cells-carrying-rspo3-fusions
#11
Gabriele Picco, Consalvo Petti, Alessia Centonze, Erica Torchiaro, Giovanni Crisafulli, Luca Novara, Andrea Acquaviva, Alberto Bardelli, Enzo Medico
In colorectal cancer (CRC), WNT pathway activation by genetic rearrangements of RSPO3 is emerging as a promising target. However, its low prevalence severely limits availability of preclinical models for in-depth characterization. Using a pipeline designed to suppress stroma-derived signal, we find that RSPO3 "outlier" expression in CRC samples highlights translocation and fusion transcript expression. Outlier search in 151 CRC cell lines identified VACO6 and SNU1411 cells as carriers of, respectively, a canonical PTPRK(e1)-RSPO3(e2) fusion and a novel PTPRK(e13)-RSPO3(e2) fusion...
January 18, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28097933/inflammatory-myopathy-in-a-patient-with-collagen-vi-mutations
#12
R Papa, C Fiorillo, C Malattia, F Minoia, R Caorsi, S Assereto, M Iacomino, M Savarese, V Nigro, C Bruno, C Minetti, P Picco
No abstract text is available yet for this article.
January 18, 2017: Scandinavian Journal of Rheumatology
https://www.readbyqxmd.com/read/28059783/improved-cerebrospinal-fluid-based-discrimination-between-alzheimer-s-disease-patients-and-controls-after-correction-for-ventricular-volumes
#13
Linda J C van Waalwijk van Doorn, Juan D Gispert, H Bea Kuiperij, Jurgen A H R Claassen, Andrea Arighi, Inês Baldeiras, Kaj Blennow, Marco Bozzali, Miguel Castelo-Branco, Enrica Cavedo, Derya D Emek-Savaş, Erden Eren, Paolo Eusebi, Lucia Farotti, Chiara Fenoglio, Juan Fortea Ormaechea, Yvonne Freund-Levi, Giovanni B Frisoni, Daniela Galimberti, Sermin Genc, Viviana Greco, Harald Hampel, Sanna-Kaisa Herukka, Yawu Liu, Albert Lladó, Alberto Lleó, Flavio M Nobili, Kader K Oguz, Lucilla Parnetti, João Pereira, Agnese Picco, Maria Pikkarainen, Catarina Resende de Oliveira, Esen Saka, Nicola Salvadori, Raquel Sanchez-Valle, Isabel Santana, Elio Scarpini, Philip Scheltens, Hilkka Soininen, Roberto Tarducci, Charlotte Teunissen, Magda Tsolaki, Andrea Urbani, Eduard Vilaplana Martinez, Pieter Jelle Visser, Asa K Wallin, Görsev Yener, José L Molinuevo, Olga Meulenbroek, Marcel M Verbeek
Cerebrospinal fluid (CSF) biomarkers may support the diagnosis of Alzheimer's disease (AD). We studied if the diagnostic power of AD CSF biomarker concentrations, i.e., Aβ42, total tau (t-tau), and phosphorylated tau (p-tau), is affected by differences in lateral ventricular volume (VV), using CSF biomarker data and magnetic resonance imaging (MRI) scans of 730 subjects, from 13 European Memory Clinics. We developed a Matlab-algorithm for standardized automated segmentation analysis of T1 weighted MRI scans in SPM8 for determining VV, and computed its ratio with total intracranial volume (TIV) as proxy for total CSF volume...
December 3, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28052020/the-rna-binding-protein-esrp1-promotes-human-colorectal-cancer-progression
#14
Sharmila Fagoonee, Gabriele Picco, Francesca Orso, Arrigo Arrigoni, Dario L Longo, Marco Forni, Irene Scarfò, Adele Cassenti, Roberto Piva, Paola Cassoni, Lorenzo Silengo, Emanuela Tolosano, Silvio Aime, Daniela Taverna, Pier Paolo Pandolfi, Mara Brancaccio, Enzo Medico, Fiorella Altruda
Epithelial splicing regulatory protein 1 (ESRP1) is an epithelial cell-specific RNA binding protein that controls several key cellular processes, like alternative splicing and translation. Previous studies have demonstrated a tumor suppressor role for this protein. Recently, however, a pro-metastatic function of ESRP1 has been reported. We thus aimed at clarifying the role of ESRP1 in Colorectal Cancer (CRC) by performing loss- and gain-of-function studies, and evaluating tumorigenesis and malignancy with in vitro and in vivo approaches...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28050895/passive-leg-raise-testing-effectively-reduces-fluid-administration-in-septic-shock-after-correction-of-non-compliance-to-test-results
#15
Arjanne Rameau, Eldert de With, Evert Christiaan Boerma
BACKGROUND: Fluid resuscitation is considered a cornerstone of shock treatment, but recent data have underlined the potential hazards of fluid overload. The passive leg raise (PLR) test has been introduced as one of many strategies to predict 'fluid responsiveness.' The use of PLR testing is applicable to a wide range of clinical situations and has the potential to reduce fluid administration, since PLR testing is based upon (reversible) autotransfusion. Despite these theoretical advantages, data on the net effect on fluid balance as a result of PLR testing remain scarce...
December 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/28039242/non-invasive-assessment-of-fluid-responsiveness-using-cnap%C3%A2-technology-is-interchangeable-with-invasive-arterial-measurements-during-major-open-abdominal-surgery
#16
J Renner, M Gruenewald, M Hill, L Mangelsdorff, H Aselmann, C Ilies, M Steinfath, O Broch
BACKGROUND: Dynamic variables of fluid responsiveness (FR), such as pulse pressure variation (PPV), have been shown to predict the response to a fluid challenge accurately. A recently introduced non-invasive technology based on the volume-clamp method (CNAP™) offers the ability to measure PPV continuously (PPVCNAP). However, the accuracy regarding the prediction of FR in the operating room has to be proved. METHODS: We compared PPVCNAP with an invasive approach measuring PPV using the PiCCO technology (PPVPiCCO)...
January 2017: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/28017278/three-dimensional-transoesophageal-echocardiography-for-cardiac-output-in-critically-ill-patients-a-pilot-study-of-ultrasound-versus-the-thermodilution-method
#17
COMPARATIVE STUDY
Nadjib Hammoudi, Guillaume Hékimian, Florent Laveau, Marc Achkar, Richard Isnard, Alain Combes
BACKGROUND: Three-dimensional transoesophageal echocardiography (3D-TOE) is a new noninvasive tool for quantitative assessment of left ventricular (LV) volumes and ejection fraction. AIM: The objective of this pilot study was to evaluate the feasibility and accuracy of 3D-TOE for the estimation of cardiac output (CO), using transpulmonary thermodilution with the Pulse index Contour Continuous Cardiac Output (PiCCO) system as the reference method, in intensive care unit (ICU) patients...
January 2017: Archives of Cardiovascular Diseases
https://www.readbyqxmd.com/read/27927259/association-between-recognition-and-help-seeking-preferences-and-stigma-towards-people-with-mental-illness
#18
L Picco, E Abdin, S Pang, J A Vaingankar, A Jeyagurunathan, S A Chong, M Subramaniam
AIMS: The ability to recognise a mental illness has important implications as it can aid in timely and appropriate help-seeking, and ultimately improve outcomes for people with mental illness. This study aims to explore the association between recognition and help-seeking preferences and stigmatising attitudes, for alcohol abuse, dementia, depression, obsessive-compulsive disorder (OCD) and schizophrenia, using a vignette-based approach. METHODS: This was a population-based, cross-sectional survey conducted among Singapore Residents (n = 3006) aged 18-65 years...
December 8, 2016: Epidemiology and Psychiatric Sciences
https://www.readbyqxmd.com/read/27922142/prevalence-and-risk-factors-of-caregiver-dependence-among-older-adults-in-a-southeast-asian-population
#19
Louisa Picco, Edimansyah Abdin, Janhavi A Vaingankar, Shirlene Pang, Saleha Shafie, Rajeswari Sambasivam, Siow Ann Chong, Mythily Subramaniam
INTRODUCTION: Currently very little is known about the prevalence or magnitude of caregiver dependence in Singapore and thus, there is a need to fill this gap in this multiethnic ageing population. This study aims to determine the prevalence and risk factors of caregiver dependence among older adults in Singapore. MATERIALS AND METHODS: Data were used from the Well-being of the Singapore Elderly (WiSE) study, a nationally representative, cross-sectional survey among Singapore residents aged 60 years and above...
November 2016: Annals of the Academy of Medicine, Singapore
https://www.readbyqxmd.com/read/27893796/attitudes-to-mental-illness-and-its-demographic-correlates-among-general-population-in-singapore
#20
Qi Yuan, Edimansyah Abdin, Louisa Picco, Janhavi Ajit Vaingankar, Shazana Shahwan, Anitha Jeyagurunathan, Vathsala Sagayadevan, Saleha Shafie, Jenny Tay, Siow Ann Chong, Mythily Subramaniam
BACKGROUND: Public attitudes to mental illness could influence how the public interact with, provide opportunities for, and help people with mental illness. AIMS: This study aims to explore the underlying factors of the Attitudes to Mental Illness questionnaire among the general population in Singapore and the socio-demographic correlates of each factor. METHODS: From March 2014 to April 2015, a nation-wide cross-sectional survey on mental health literacy with 3,006 participants was conducted in Singapore...
2016: PloS One
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