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protamine influence

Bruno P Meloni, Diego Milani, Jane L Cross, Vince W Clark, Adam B Edwards, Ryan S Anderton, David J Blacker, Neville W Knuckey
We have demonstrated that arginine-rich and poly-arginine peptides possess potent neuroprotective properties with arginine content and peptide positive charge being particularly critical for neuroprotective efficacy. In addition, the presence of other amino acids within arginine-rich peptides, as well as chemical modifications, peptide length and cell-penetrating properties also influence the level of neuroprotection. Against this background, we have examined the neuroprotective efficacy of arginine-rich protamine peptides, a cyclic (R12-c) poly-arginine peptide and a R22 poly-arginine peptide, as well as arginine peptides containing tryptophan or other amino acids (phenylalanine, tyrosine, glycine or leucine) in in vitro glutamic acid excitotoxicity and in vivo rat permanent middle cerebral artery occlusion models of stroke...
May 18, 2017: Neuromolecular Medicine
Arumugam Kumaresan, Anders Johannisson, Sarah Nordqvist, Karin Kårehed, Helena Åkerud, Karin Lindgren, Jane Morrell
The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or HRG C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n=118) undergoing IVF or ICSI. Sperm DNA integrity was analyzed by flow cytometry using three fluorescent probes (Acridine Orange, Monobromobimane and Chromomycin A3). Principal component analysis was used to identify the parameters that most influenced fertility...
March 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
Wolfgang Rathmann, Marcin Czech, Edward Franek, Karel Kostev
AIMS: To compare short-term basal insulin therapy persistence and its predictors in Poland and Germany. METHODS: Persistence was defined as proportions of patients remaining on the initial basal insulin (analogs: Poland: n = 6,889, Germany: n = 454,067; neutral protamine Hagedorn (NPH) insulins: Poland: n = 50,761, Germany: n = 226,064) over 2 years based on nationwide prescription databases (LRx; IMS Health) in Poland and Germany from 2013 to 2015. Persistence was evaluated by Kaplan-Meier curves (log-rank tests)...
February 2017: International Journal of Clinical Pharmacology and Therapeutics
Bernhard Scheicher, Cornelia Lorenzer, Katrin Gegenbauer, Julia Partlic, Fritz Andreae, Alexander H Kirsch, Alexander R Rosenkranz, Oliver Werzer, Andreas Zimmer
Since therapeutic peptides and oligonucleotides are gathering interests as active pharmaceutical ingredients (APIs), nanoparticulate drug delivery systems are becoming of great importance. Thereby, the possibility to design drug delivery systems according to the therapeutic needs of APIs enhances clinical implementation. Over the last years, the focus of our group was laid on protamine-oligonucleotide-nanoparticles (so called proticles), however, the possibility to modify the size, zeta potential or loading efficiencies was limited...
2016: PloS One
B H Kipper, J T Trevizan, J T Carreira, I R Carvalho, G Z Mingoti, M E Beletti, S H V Perri, D A Franciscato, J C Pierucci, M B Koivisto
The aim of this study was to evaluate the chromatin packing and sperm head morphometry of cryopreserved semen of Nelore bulls (Bos taurus indicus) of different ages. Furthermore, the influence of the degree of chromatin compaction on in vitro embryo production (IVP) was investigated. Forty bulls were divided into three groups: young (1.8-2 years), adult (3.5-7 years), and senile (8-14.3 years). The ejaculates were frozen according to standards established by the Artificial Insemination Center located in the Southeast of Brazil...
January 1, 2017: Theriogenology
Xinliang Guan, Jiachen Li, Ming Gong, Feng Lan, Hongjia Zhang
Coagulopathy is still a frequent complication in the surgical treatment of acute aortic dissection. However, the physiopathology of surgically induced coagulopathy has never been systematically and comprehensively studied in patients with acute aortic dissection. The aim of the present study was to describe the perioperative hemostatic system in patients with acute aortic dissection.The 87 patients who underwent aortic arch surgery for acute Stanford type A aortic dissection from January 2013 to September 2015 were enrolled in this study...
September 2016: Medicine (Baltimore)
M I Meesters, G Kuiper, A B A Vonk, S A Loer, C Boer
Point-of-care coagulation monitoring can be used for the guidance of haemostasis management. However, the influence of time on point-of-care prothrombin time testing following protamine administration after cardiopulmonary bypass has not been investigated. Bland-Altman and error grid analysis were used to analyse the level of agreement between prothrombin time measurements from point-of-care and laboratory tests before cardiopulmonary bypass, and then 3 min, 6 min and 10 min after protamine administration. Prothrombin times were expressed as International Normalised Ratios...
October 2016: Anaesthesia
Caroline Van Aelbrouck, Sebastian Jorquera-Vasquez, Ingrid Beukinga, Olivier Pradier, Brigitte Ickx, Luc Barvais, Luc Van Obbergh, David Faraoni
This study sought to compare the effect of tranexamic acid (TXA) administration on cardiopulmonary bypass-induced platelet dysfunction in patients who received preoperative aspirin or not. We performed a prospective, randomized, double-blind pilot study, including patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB). Patients without aspirin interruption were enrolled in the 'group ASPIRIN' (n = 18) and those who had never been treated with aspirin were included in the 'group NO ASPIRIN' (n = 10)...
December 2016: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
Undraga Schagdarsurengin, Patrick Western, Klaus Steger, Andreas Meinhardt
Male gamete development begins with the specification of primordial cells in the epiblast of the early embryo and is not complete until spermatozoa mature in the epididymis of adult males. This protracted developmental process involves extensive alteration of the paternal germline epigenome. Initially, epigenetic reprogramming in fetal germ cells results in removal of most DNA methylation, including parent-specific epigenetic information. The germ cells then establish sex-specific epigenetic information through de novo methylation and undergo spermatogenesis...
June 17, 2016: Seminars in Immunopathology
Michael I Meesters, Dennis Veerhoek, Fellery de Lange, Jacob-Willem de Vries, Jan R de Jong, Johannes W A Romijn, Hilde Kelchtermans, Dana Huskens, Robin van der Steeg, Pepijn W A Thomas, David T M Burtman, Laurentius J M van Barneveld, Alexander B A Vonk, Christa Boer
While experimental data state that protamine exerts intrinsic anticoagulation effects, protamine is still frequently overdosed for heparin neutralisation during cardiac surgery with cardiopulmonary bypass (CPB). Since comparative studies are lacking, we assessed the influence of two protamine-to-heparin dosing ratios on perioperative haemostasis and bleeding, and hypothesised that protamine overdosing impairs the coagulation status following cardiac surgery. In this open-label, multicentre, single-blinded, randomised controlled trial, patients undergoing on-pump coronary artery bypass graft surgery were assigned to a low (0...
August 1, 2016: Thrombosis and Haemostasis
Mark A Chaney, J Devin Roberts, Kristen Wroblewski, Sajid Shahul, Ross Gaudet, Valuvan Jeevanandam
OBJECTIVE: The method of protamine administration may influence adverse reactions. The authors investigated the effects of 3 different methods of protamine administration on cardiopulmonary function. DESIGN: Prospective, randomized clinical study. SETTING: Single university hospital. PARTICIPANTS: Human volunteer patients. INTERVENTIONS: Ninety-five patients undergoing cardiac surgery were randomized prospectively into 3 groups...
June 2016: Journal of Cardiothoracic and Vascular Anesthesia
Majid Ghahraman Afshar, Gastón A Crespo, Eric Bakker
We report here on the development of a chronopotentiometric readout for ion-selective electrodes that allows one to record transition times in continuous flow conditions without the necessity to stop the flow. A sample plug of 150 μL is injected into the carrier solution (0.5 mM NaCl) and subsequently transported to the detection cell (∼20 μL) at moderate flow rates (∼0.5 mL min(-1)), where a short current pulse (5s) is applied between the ionophore-based working electrode and a biocompatible and nonpolarizable Donnan exclusion anion-exchanger membrane reference/counter electrode...
April 5, 2016: Analytical Chemistry
Ya G Voronkova, T N Popova, A A Agarkov, M V Skulachev
An influence of 10-(6'-plastoquinonyl)decyltriphenylphosphonium (SkQ1) on oxidative status and activity of some antioxidant enzymes in the liver and blood serum from rats was examined during experimental hyperglycemia developed after injecting protamine sulfate. It was found that SkQ1 lowered glycemic level in rats treated with protamine sulfate. Moreover, it was also accompanied by restoration of the normal range of biochemiluminescence parameters indicating the rate of ongoing free radical processes, magnitude of primary products of lipid peroxidation such as diene conjugates, activity of aconitate hydratase, and level of citrate in rat liver and blood...
December 2015: Biochemistry. Biokhimii︠a︡
Lena Lüke, Maximiliano Tourmente, Eduardo R S Roldan
Protamines have a crucial role in male fertility. They are involved in sperm chromatin packaging and influence the shape of the sperm head and, hence, are important for sperm performance. Protamine structure is basic with numerous arginine-rich DNA-binding domains. Postcopulatory sexual selection is thought to play an important role in protamine sequence evolution and expression. Here, we analyze patterns of evolution and sexual selection (in the form of sperm competition) acting on protamine 1 gene sequence in 237 mammalian species...
January 2016: Molecular Biology and Evolution
Philippe Grieshaber, Tamam Bakchoul, Jochen Wilhelm, Alexander Wagner, Matthias Wollbrück, Andreas Böning, Ulrich Sachs
OBJECTIVES: Platelet-activating antibodies against protamine-heparin-complexes were described in patients undergoing cardiac surgery, but their clinical consequences remain unclear. This prospective single-center observational study aimed to describe the prevalence and clinical consequences of protamine-heparin-complex antibodies in patients undergoing cardiac surgery with cardiopulmonary bypass. METHODS: A total of 200 patients undergoing cardiac surgery with cardiopulmonary bypass were included...
October 2015: Journal of Thoracic and Cardiovascular Surgery
Lien M Davidson, Kate Millar, Celine Jones, Muhammad Fatum, Kevin Coward
Worldwide obesity rates have nearly doubled since 1980 and currently over 10% of the population is obese. In 2008, over 1.4 billion adults aged 20 years and older had a body mass index or BMI above a healthy weight and of these, over 200 million men and nearly 300 million women were obese. While obesity can have many ramifications upon adult life, one growing area of concern is that of reproductive capacity. Obesity affects male infertility by influencing the hypothalamic-pituitary-gonadal axis, thus causing detrimental effects upon spermatogenesis and subsequent fertility...
September 2015: Human Fertility: Journal of the British Fertility Society
Michael Isaäc Meesters, Marcus Daniel Lancé, Robin van der Steeg, Christa Boer
UNLABELLED: The thromboelastometry INTEM clotting time (CT) with heparinase (HEPTEM) is frequently used to detect residual heparin after cardiopulmonary bypass (CPB) in cardiac surgery. This study investigated whether the HEPTEM CT reflects the presence of residual heparin and the association of the protamine-to-heparin ratio to the INTEM and HEPTEM CT. We retrospectively evaluated thromboelastometry data that were obtained before CPB and after protamine infusion following CPB in two tertiary hospitals...
November 2015: Thrombosis and Haemostasis
Saeed Kolahian, Hassan Sadri, Amir Ali Shahbazfar, Morvarid Amani, Anis Mazadeh, Mehdi Mirani
Diabetes mellitus is a major cause of serious micro- and macrovascular diseases that affect nearly every system in the body, including the respiratory system. Non-enzymatic protein glycation due to hyperglycaemic stress has fundamental implications due to the large capillary network and amount of connective tissue in the lung. The current study was designed to determine whether leucine, zinc, and chromium supplementations influence the function and histological structure of the respiratory tract in a rat model of type 2 diabetes...
2015: PloS One
Vladimir Radulovic, Anna Laffin, Kenny M Hansson, Erika Backlund, Fariba Baghaei, Anders Jeppsson
BACKGROUND: Bleeding complications are common in cardiac surgery. Perioperative handling of heparin and protamine may influence the haemostasis. We hypothesized that heparin and protamine dosing based on individual titration curves would improve haemostasis in comparison to standard dosing. SUBJECTS AND METHODS: Sixty patients scheduled for first time elective coronary artery bypass grafting or valve surgery were included in a prospective randomized study. The patients were randomized to heparin and protamine dosing with Hepcon HMS Plus device or to standard weight and activated clotting time (ACT) based dosing...
2015: PloS One
Ali A Ensafi, N Kazemifard, B Rezaei
A novel fluorescent biosensor is developed, based on glutathione-capped CdTe quantum dots aggregation, for the determination of trace amount of an important drug, protamine. In this method with increasing the protamine concentration, the fluorescence of the quantum dots was quenched due to their aggregation. Different parameters affect the sensitivity, such as pH and the amount of the quantum dots, were optimized. Using the new optical biosensor, under the optimized conditions, protamine could be measured in the range of 2...
September 15, 2015: Biosensors & Bioelectronics
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