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Diagnostic of TB

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https://www.readbyqxmd.com/read/27924983/detection-of-the-tuberculosis-antigenic-marker-mannose-capped-lipoarabinomannan-in-pretreated-serum-by-surface-enhanced-raman-scattering
#1
Alexis C Crawford, Lars B Laurentius, Timothy S Mulvihill, Jennifer H Granger, John S Spencer, Delphi Chatterjee, Kimberly E Hanson, Marc D Porter
The ability to detect tuberculosis (TB) continues to be a global health care priority. This paper describes the development and preliminary assessment of the clinical accuracy of a heterogeneous immunoassay that integrates a serum pretreatment process with readout by surface-enhanced Raman scattering (SERS) for the low-level detection of mannose-capped lipoarabinomannan (ManLAM). ManLAM is a major virulence factor in the infectious pathology of Mycobacterium tuberculosis (Mtb) that has been found in the serum and other body fluids of infected patients...
December 7, 2016: Analyst
https://www.readbyqxmd.com/read/27919693/performance-evaluation-of-xpert-mtb-rif-in-a-moderate-tuberculosis-incidence-compared-with-taqman-mtb-and-trcrapid-m-tb
#2
Kazunari Tsuyuguchi, Hideaki Nagai, Kenji Ogawa, Tomoshige Matsumoto, Kozo Morimoto, Akiko Takaki, Satoshi Mitarai
Xpert MTB/RIF is an automated nucleic acid amplification test (NAT) that can detect the presence of Mycobacterium tuberculosis complex (MTC) in clinical specimens as well as rifampicin (RIF) resistance resulting from rpoB mutation. Despite its high sensitivity and specificity for diagnosing tuberculosis (TB) with or without RIF resistance, the clinical performance of the test is variable. In this study, we evaluated the performance of Xpert MTB/RIF in a setting of moderate TB burden and high medical resources...
December 2, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/27913043/evaluation-of-the-genexpert-mtb-rif-assay-on-extrapulmonary-and-respiratory-samples-other-than%C3%A2-sputum-a-low-burden-country-experience
#3
Sushil Pandey, Jacob Congdon, Bradley McInnes, Alina Pop, Christopher Coulter
The aim of this study was to assess the performance of the GeneXpert MTB/RIF assay on extrapulmonary (EP) and respiratory (non-sputum) clinical samples of patients suspected of having tuberculosis (TB) from Queensland, Australia. A total of 269 EP and respiratory (non-sputum) clinical samples collected from Qld patients who were suspected of having TB were subjected to the GeneXpert MTB/RIF analysis, Ziehl-Neelsen (ZN) staining, Mycobacterium tuberculosis (MTB) culture and drug susceptibility testing. Phenotypic and genotypic data were compared...
November 29, 2016: Pathology
https://www.readbyqxmd.com/read/27911922/nucleic-acid-amplification-testing-and-sequencing-combined-with-acid-fast-staining-in-needle-biopsy-lung-tissues-for-the-diagnosis-of-smear-negative-pulmonary-tuberculosis
#4
Faming Jiang, Weiwei Huang, Ye Wang, Panwen Tian, Xuerong Chen, Zongan Liang
BACKGROUND: Smear-negative pulmonary tuberculosis (PTB) is common and difficult to diagnose. In this study, we investigated the diagnostic value of nucleic acid amplification testing and sequencing combined with acid-fast bacteria (AFB) staining of needle biopsy lung tissues for patients with suspected smear-negative PTB. METHODS: Patients with suspected smear-negative PTB who underwent percutaneous transthoracic needle biopsy between May 1, 2012, and June 30, 2015, were enrolled in this retrospective study...
2016: PloS One
https://www.readbyqxmd.com/read/27903602/whole-genome-sequencing-of-mycobacterium-tuberculosis-provides-insight-into-the-evolution-and-genetic-composition-of-drug-resistant-tuberculosis-in-belarus
#5
Kurt R Wollenberg, Christopher A Desjardins, Aksana Zalutskaya, Vervara Slodovnikova, Andrew J Oler, Mariam Quiñones, Thomas Abeel, Sinead B Chapman, Michael Tartakovsky, Andrei Gabrielian, Sven Hoffner, Aliaksandr Skrahin, Bruce W Birren, Alexander Rosenthal, Alena Skrahina, Ashlee M Earl
The emergence and spread of drug resistant Mycobacterium tuberculosis (DR-TB) is a critical global health issue. Eastern Europe has some of the highest incidences of DR-TB, particularly multidrug-resistant (MDR) and extensively drug-resistant (XDR) TB. To better understand the genetic composition and evolution of MDR- and XDR-TB in the region, we sequenced and analyzed the genomes of 138 M. tuberculosis isolates from 97 patients sampled between 2010 and 2013 in Minsk, Belarus. MDR and XDR-TB isolates were significantly more likely to belong to the Beijing lineage than to the Euro-American lineage, and known resistance-conferring loci accounted for the majority of phenotypic resistance to first- and second-line drugs in MDR and XDR-TB...
November 30, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27895634/cytosolic-proteome-profiling-of-aminoglycosides-resistant-mycobacterium-tuberculosis-clinical-isolates-using-maldi-tof-ms
#6
Divakar Sharma, Manju Lata, Rananjay Singh, Nirmala Deo, Krishnamurthy Venkatesan, Deepa Bisht
Emergence of extensively drug resistant tuberculosis (XDR-TB) is the consequence of the failure of second line TB treatment. Aminoglycosides are the important second line anti-TB drugs used to treat the multi drug resistant tuberculosis (MDR-TB). Main known mechanism of action of aminoglycosides is to inhibit the protein synthesis by inhibiting the normal functioning of ribosome. Primary target of aminoglycosides are the ribosomal RNA and its associated proteins. Various mechanisms have been proposed for aminoglycosides resistance but still some are unsolved...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27894266/tuberculosis-drug-resistance-in-bamako-mali-from-2006-to-2014
#7
B Diarra, D Goita, S Tounkara, M Sanogo, B Baya, A C G Togo, M Maiga, Y S Sarro, A Kone, B Kone, O M'Baye, N Coulibaly, H Kassambara, A Cisse, M Belson, M A Polis, J Otu, F Gehre, M Antonio, S Dao, S Siddiqui, R L Murphy, B C de Jong, S Diallo
BACKGROUND: Although Drug resistance tuberculosis is not a new phenomenon, Mali remains one of the "blank" countries without systematic data. METHODS: Between 2006 and 2014, we enrolled pulmonary TB patients from local TB diagnostics centers and a university referral hospital in several observational cohort studies. These consecutive patients had first line drug susceptibility testing (DST) performed on their isolates. A subset of MDR was subsequently tested for second line drug resistance...
November 28, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27888179/predicting-tuberculosis-risk-in-the-foreign-born-population-of-british-columbia-canada-study-protocol-for-a-retrospective-population-based-cohort-study
#8
Lisa A Ronald, Jonathon R Campbell, Robert F Balshaw, David Z Roth, Kamila Romanowski, Fawziah Marra, Victoria J Cook, James C Johnston
INTRODUCTION: Improved understanding of risk factors for developing active tuberculosis (TB) will better inform decisions about diagnostic testing and treatment for latent TB infection (LTBI) in migrant populations in low-incidence regions. We aim to examine TB risk factors among the foreign-born population in British Columbia (BC), Canada, and to create and validate a clinically relevant multivariate risk score to predict active TB. METHODS AND ANALYSIS: This retrospective population-based cohort study will include all foreign-born individuals who acquired permanent resident status in Canada between 1 January 1985 and 31 December 2013 and acquired healthcare coverage in BC at any point during this period...
November 25, 2016: BMJ Open
https://www.readbyqxmd.com/read/27888000/enhanced-laboratory-capacity-development-a-boost-for-effective-tuberculosis-control-in-resource-limited-settings
#9
Abraham Sunday Alabi, Afsatou Ndama Traoré, Marguerite Massinga Loembe, Ulysse Ateba-Ngoa, Matthias Frank, Ayola Akim Adegnika, Bertrand Lell, Jocelyn Mahoumbou, Carsten Köhler, Peter Gottfried Kremsner, Martin Peter Grobusch
Both routine and research tuberculosis (TB) laboratory capacity urgently need to be expanded in large parts of Sub-Saharan Africa. In 2009, the Centre de Recherches Médicales de Lambaréné (CERMEL) took a strategic decision to expand its activities by building TB laboratory capacity to address research questions and to improve routine diagnostic and treatment capacity. Over the past 7 years, a standard laboratory has been developed that is contributing significantly to TB diagnosis, treatment, and control in Gabon; training has also been provided for TB research staff in Central Africa...
November 22, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27887611/the-performance-of-interferon-gamma-release-assay-in-nontuberculous-mycobacterial-diseases-a-retrospective-study-in-china
#10
Mao-Shui Wang, Jun-Li Wang, Xin-Feng Wang
BACKGROUND: The interferon-gamma release assay (IGRA) is more specific than the tuberculin skin test to discriminate between tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases. Here we performed a retrospective study to evaluate the performance of the T-SPOT.TB in patients with NTM diseases. METHODS: Between March, 2013 and Nov, 2015, a total of 58 patients with NTM diseases had a T-SPOT.TB performed were enrolled, 30 patients had definite NTM diseases, 28 had probable diseases...
November 25, 2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27882115/retrospective-analysis-of-seven-breast-tuberculosis-cases
#11
Beyhan Çakar, Aydin Çiledağ
The present study aimed to evaluate the demographic data, diagnostic methods, therapeutic regimens and duration of therapy in 7 breast tuberculosis (BTB) cases. The data of BTB cases treated between January 2006 and December 2013 were retrospectively evaluated, with a total of 648 tuberculosis (TB) cases recorded during the 8-year period. Among these cases, 296 patients (50%) suffered from pulmonary TB, 278 (43%) from non-pulmonary TB and 45 (7%) from PTB plus NPTB. In total, 7 BTB were diagnosed, which constituted 1...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27872016/tuberculosis-eradication-versus-control
#12
Marco Schito, Debra Hanna, Alimuddin Zumla
According to the World Health Organization (WHO), 10.4 million people succumbed to tuberculosis (TB) in 2015 and is now the number one cause of death world-wide from a preventable infectious disease. Bold vision is needed from global leaders and plans have been proposed to end the TB epidemic. However enthusiasm must be matched by tangible and achievable goals based on the science and available funding. In order to reach the target and goals set by the WHO's End-TB Strategy, the challenges for TB eradication need to be addressed...
November 18, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27865395/innovative-tuberculosis-symposium-held-during-cuba-salud-2015
#13
EDITORIAL
Helena J Chapman, Luisa Armas Pérez
The fourth Tuberculosis (TB) Symposium, held during the Cuba Salud 2015 International Convention, highlighted advancements in research on TB and Mycobacterium tuberculosis (Mtb) by interdisciplinary teams from academic and federal institutions in Cuba, Colombia, Mexico, and the Dominican Republic. Delegates focused on the targets presented in the World Health Organization End TB Strategy for 2016-2035 and elaborated on four primary themes: 1) attention to vulnerable populations such as immunocompromised individuals, health care workers, and residents of long-term institutions such as prisons and nursing homes; 2) identification of active and latent TB cases through contact investigations; 3) spread and control of drug-resistant Mtb strains; and 4) advancements in the development of novel vaccines or "booster" immunizations...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27865378/development-of-immune-biomarkers-of-pulmonary-tuberculosis-in-a-rabbit-model
#14
Ali Dehnad, Resmi Ravindran, Selvakumar Subbian, Imran H Khan
Tuberculosis (TB) causes extensive morbidity and mortality worldwide with approximately 10 million new cases of active disease emerging mostly from a pool of two billion individuals latently infected with Mycobacterium tuberculosis (M. tb) every year. The underlying host immune responses that drive M. tb infection to active disease or latency are not well understood. We propose that identification and characterization of host immune biomarkers will be helpful to better understand the mechanisms that drive this process, and may, in addition, lead to the development of better diagnostic tools for TB...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27865236/pcr-targeting-is6110-in-diagnosing-tuberculosis-in-children-in-comparison-to-mgit-culture
#15
Rajeshwar Dayal, Dipti Agarwal, Harish Pathak, Shehraj Feroz, Manoj Kumar, Devendra S Chauhan, Rakesh Bhatia
BACKGROUND: Diagnosis of tuberculosis (TB) in children is difficult in children especially in extrapulmonary tuberculosis (EPTB). This study was conducted to evaluate the use of polymerase chain reaction (PCR) targeting IS6110 in the diagnosis of TB in children with pulmonary TB and EPTB and also to compare its performance with MGIT 960 culture and conventional microscopy. METHODS: A total of 142 cases (50 pulmonary, 92 extrapulmonary) of suspected TB patients <15 years of age were included in the study...
July 2016: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/27863623/tuberculous-lymphadenitis-of-the-head-and-neck-in-canadian-children-experience-from-a-low-burden-region
#16
Jason J Xu, Shazia Peer, Blake C Papsin, Ian Kitai, Evan J Propst
BACKGROUND: Children are more likely than adults to develop extra-pulmonary tuberculosis (EPTB), which often presents as cervical lymphadenopathy. The role of surgery in management is uncertain. We reviewed all head and neck EPTB cases presenting to our tertiary care pediatric institution over a twelve-year period. METHODS: All children 18 years of age and younger with EPTB involving the head and neck were included. We recorded clinical data and age at diagnosis, birth country, BCG vaccination status, as well as radiographic, surgical, histological, and microbiological results...
December 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/27863556/oral-vaccination-of-cattle-with-heat-inactivated-mycobacterium-bovis-does-not-compromise-bovine-tb-diagnostic-tests
#17
Gareth J Jones, Sabine Steinbach, Iker A Sevilla, Joseba M Garrido, Ramon Juste, H Martin Vordermeier
In this study we investigated whether oral uptake of a heat inactivated M. bovis wildlife vaccine by domestic cattle induced systemic immune responses that compromised the use of tuberculin or defined antigens in diagnostic tests for bovine TB. Positive skin test and blood-based IFN-γ release assay (IGRA) results were observed in all calves vaccinated via the parenteral route (i.e. intramuscular). In contrast, no positive responses to tuberculin or defined antigens were observed in either the skin test or IGRA test when performed in calves vaccinated via the oral route...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27863555/test-performance-of-three-serological-assays-for-the-detection-of-mycobacterium-bovis-infection-in-common-warthogs-phacochoerus-africanus
#18
Eduard O Roos, Peter Buss, Lin-Mari de Klerk-Lorist, Jennie Hewlett, Guy A Hausler, Leana Rossouw, Alicia J McCall, David Cooper, Paul D van Helden, Sven D C Parsons, Michele A Miller
Sporadic cases of bovine tuberculosis (bTB) have been reported in warthogs in Southern Africa and confirmed through mycobacterial culture. However, there are no validated ante-mortem tests currently available for bTB in warthogs. In this study, we evaluated the use of three serological assays for the detection of Mycobacterium bovis infection in warthogs; an indirect enzyme-linked immunosorbent assay (ELISA) using bovine purified protein derivative (PPDb) as a capture antigen (indirect PPD ELISA), as well as two commercial assays, the TB ELISA-VK(®) and DPP(®) VetTB Assay...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27858862/utility-of-t-cell-interferon-%C3%AE-release-assays-for-the-diagnosis-of-female-genital-tuberculosis-in-a-tertiary-referral-hospital-in-beijing-china
#19
Xiaoqing Liu, Sainan Bian, Xinhe Cheng, Wenze Wang, Qinjie Tian, Lifan Zhang, Yueqiu Zhang, Xiaochun Shi, Yao Zhang, Zhiyong Liang
Diagnosis of female genital tuberculosis (FGTB) remains a challenge. The aim of this study was to evaluate the diagnostic value of T-SPOT.TB on peripheral blood mononuclear cells (PBMCs) for diagnosing FGTB in an area with high TB burden.Patients with suspected FGTB were enrolled consecutively between August 2010 and August 2015. T-SPOT.TB on PBMCs and histopathology were performed in all patients. T-SPOT.TB results were evaluated against patients' final diagnosis of FGTB which was made based on clinical manifestations, radiology, microbiological and histopathological evaluation, and response to anti-TB treatment...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27857829/diagnostic-delay-among-adults-with-pulmonary-tuberculosis-in-a-high-gross-domestic-product-per-capita-country-reasons-and-magnitude-of-the-problem
#20
Wanis H Ibrahim, Faraj H Alousi, Abdulatif Al-Khal, Abdulbari Bener, Ahmed AlSalman, Aaiza Aamer, Ahmed Khaled, Tasleem Raza
BACKGROUND: To determine the mean and median delays in pulmonary tuberculosis (PTB) diagnosis among adults in one of the world's highest gross domestic product per capita countries and identify patient and health system-related reasons for these delays. METHODS: This is a cross-sectional, face-to-face, prospective study of 100 subjects with confirmed PTB, conducted at main tuberculosis (TB) admitting facilities in Qatar. The mean and median diagnostic delays were measured...
2016: International Journal of Preventive Medicine
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