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fetal cell isolation

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https://www.readbyqxmd.com/read/29021611/spatial-detection-of-fetal-marker-genes-expressed-at-low-level-in-adult-human-heart-tissue
#1
Michaela Asp, Fredrik Salmén, Patrik L Ståhl, Sanja Vickovic, Ulrika Felldin, Marie Löfling, José Fernandez Navarro, Jonas Maaskola, Maria J Eriksson, Bengt Persson, Matthias Corbascio, Hans Persson, Cecilia Linde, Joakim Lundeberg
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In many studies on heart failure, cardiac biopsies have been analyzed as uniform pieces of tissue with bulk techniques, but this homogenization approach can mask medically relevant phenotypes occurring only in isolated parts of the tissue. This study examines such spatial variations within and between regions of cardiac biopsies...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28975370/mesenchymal-stem-cells-from-preterm-to-term-newborns-undergo-a-significant-switch-from-anaerobic-glycolysis-to-the-oxidative-phosphorylation
#2
Silvia Ravera, Marina Podestà, Federica Sabatini, Chiara Fresia, Marta Columbaro, Silvia Bruno, Ezio Fulcheri, Luca Antonio Ramenghi, Francesco Frassoni
We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs...
October 3, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28962704/cigarette-smoke-condensate-affects-the-retinoid-pathway-in-human-amnion
#3
Marion Rouzaire, Aurélie Comptour, Corinne Belville, Damien Bouvier, Vincent Sapin, Denis Gallot, Loïc Blanchon
INTRODUCTION: The preterm premature rupture of membranes (PPROM) is a frequent pathology responsible of more than 30% of preterm births. Tobacco smoking is one of the most frequently described risk factors identified and contributes to the pre term weakening of fetal membranes. As previously demonstrated, all-trans retinoic acid (atRA) regulates several genes involved in the extracellular matrix dynamics, an essential actor in fetal membrane ruptures. We hypothesized that cigarette smoke may affect this pathway in human amnion...
October 2017: Placenta
https://www.readbyqxmd.com/read/28962583/engineering-human-ventricular-heart-muscles-based-on-a-highly-efficient-system-for-purification-of-human-pluripotent-stem-cell-derived-ventricular-cardiomyocytes
#4
Bin Li, Hui Yang, Xiaochen Wang, Yongkun Zhan, Wei Sheng, Huanhuan Cai, Haoyang Xin, Qianqian Liang, Ping Zhou, Chao Lu, Ruizhe Qian, Sifeng Chen, Pengyuan Yang, Jianyi Zhang, Weinian Shou, Guoying Huang, Ping Liang, Ning Sun
BACKGROUND: Most infarctions occur in the left anterior descending coronary artery and cause myocardium damage of the left ventricle. Although current pluripotent stem cells (PSCs) and directed cardiac differentiation techniques are able to generate fetal-like human cardiomyocytes, isolation of pure ventricular cardiomyocytes has been challenging. For repairing ventricular damage, we aimed to establish a highly efficient purification system to obtain homogeneous ventricular cardiomyocytes and prepare engineered human ventricular heart muscles in a dish...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28960647/long-term-fate-of-human-fetal-liver-progenitor-cells-transplanted-in-injured-mouse-livers
#5
Antony Irudayaswamy, Mark Muthiah, Lei Zhou, Hau Hung, Nur Halisah Bte Jumat, Jamil Haque, Narcissus Teoh, Geoffrey Farrell, Kimberly J Riehle, Jaymie Siqi Lin, Lin Lin Su, Jerry Ky Chan, Mahesh Choolani, Peng Cheang Wong, Aileen Wee, Seng Gee Lim, Jean Campbell, Nelson Fausto, Yock Young Dan
Liver progenitor cells have the potential to repair and regenerate a diseased liver. The success of any translational efforts, however, hinges on thorough understanding of the fate of these cells after transplant, especially in terms of long-term safety and efficacy. Here, we report transplantation of a liver progenitor population isolated from human fetal livers into immune-permissive mice with follow-up up to 36 weeks after transplant. We found that human progenitor cells engraft and differentiate into functional human hepatocytes in the mouse, producing albumin, alpha-1-antitrypsin, and glycogen...
September 28, 2017: Stem Cells
https://www.readbyqxmd.com/read/28946814/chd1l-promotes-neuronal-differentiation-in-human-embryonic-stem-cells-by-upregulating-pax6
#6
Dandan Dou, Hao Zhao, Zili Li, Liping Xu, Xifeng Xiong, Xingwu Wu, Yi Sun, Sicong Zeng, Qi Ouyang, Di Zhou, Ningfang Ma, Ge Lin, Liang Hu
Chromodomain helicase DNA binding protein 1-like gene (CHD1L) was initially isolated as a candidate oncogene in hepatocellular carcinoma, and it has been associated with many malignancies. Knockdown of Chd1l in zygote-stage mouse embryos resulted in developmental arrest, suggesting that Chd1l is required for mouse early development. However, the exact role of CHD1L in development, especially in humans, has not been reported. In this study, we found that overexpression of CHD1L in human embryonic cells (hESCs) upregulated the expression of ectoderm genes, especially PAX6...
September 25, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28936487/platelet-dense-granules-begin-to-selectively-accumulate-mepacrine-during-proplatelet-formation
#7
Hayley A Hanby, Jialing Bao, Ji-Yoon Noh, Danuta Jarocha, Mortimer Poncz, Mitchell J Weiss, Michael S Marks
Platelet dense granules (DGs) are storage organelles for calcium ions, small organic molecules such as ADP and serotonin, and larger polyphosphates that are secreted upon platelet stimulation to enhance platelet activation, adhesion, and stabilization at sites of vascular damage. DGs are thought to fully mature within megakaryocytes (MKs) prior to platelet formation. Here we challenge this notion by exploiting vital fluorescent dyes to distinguish mildly acidic DGs from highly acidic compartments by microscopy in platelets and MKs...
August 22, 2017: Blood Advances
https://www.readbyqxmd.com/read/28934223/ursodeoxycholic-acid-prevents-ventricular-conduction-slowing-and-arrhythmia-by-restoring-t-type-calcium-current-in-fetuses-during-cholestasis
#8
Oladipupo Adeyemi, Anita Alvarez-Laviada, Francisca Schultz, Effendi Ibrahim, Michael Trauner, Catherine Williamson, Alexey V Glukhov, Julia Gorelik
BACKGROUND: Increased maternal serum bile acid concentrations in intrahepatic cholestasis of pregnancy (ICP) are associated with fetal cardiac arrhythmias. Ursodeoxycholic acid (UDCA) has been shown to demonstrate anti-arrhythmic properties via preventing ICP-associated cardiac conduction slowing and development of reentrant arrhythmias, although the cellular mechanism is still being elucidated. METHODS: High-resolution fluorescent optical mapping of electrical activity and electrocardiogram measurements were used to characterize effects of UDCA on one-day-old neonatal and adult female Langendorff-perfused rat hearts...
2017: PloS One
https://www.readbyqxmd.com/read/28926198/serum-free-human-msc-medium-supports-consistency-in-human-but-not-in-equine-adipose-derived-multipotent-mesenchymal-stromal-cell-culture
#9
Susanna Schubert, Walter Brehm, Aline Hillmann, Janina Burk
For clinical applications of multipotent mesenchymal stromal cells (MSCs), serum-free culture is preferable to standardize cell products and prevent contamination with pathogens. In contrast to human MSCs, knowledge on serum-free culture of large animal MSCs is limited, despite its relevance for preclinical studies and development of veterinary cellular therapeutics. This study aimed to evaluate the suitability of a commercially available serum-free human MSC medium for culturing equine adipose-derived MSCs in comparison with human adipose MSCs...
September 19, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28923940/defective-decidualization-during-and-after-severe-preeclampsia-reveals-a-possible-maternal-contribution-to-the-etiology
#10
Tamara Garrido-Gomez, Francisco Dominguez, Alicia Quiñonero, Patricia Diaz-Gimeno, Mirhan Kapidzic, Matthew Gormley, Katherine Ona, Pablo Padilla-Iserte, Michael McMaster, Olga Genbacev, Alfredo Perales, Susan J Fisher, Carlos Simón
In preeclampsia (PE), cytotrophoblast (CTB) invasion of the uterus and spiral arteries is often shallow. Thus, the placenta's role has been a focus. In this study, we tested the hypothesis that decidual defects are an important determinant of the placental phenotype. We isolated human endometrial stromal cells from nonpregnant donors with a previous pregnancy that was complicated by severe PE (sPE). Compared with control cells, they failed to decidualize in vitro as demonstrated by morphological criteria and the analysis of stage-specific antigens (i...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28922831/the-transcription-factors-coup-tfi-and-coup-tfii-have-distinct-roles-in-arealisation-and-gabaergic-interneuron-specification-in-the-early-human-fetal-telencephalon
#11
Ayman Alzu'bi, Susan J Lindsay, Lauren F Harkin, Jack McIntyre, Steven N Lisgo, Gavin J Clowry
In human telencephalon at 8-12 postconceptional weeks, ribonucleic acid quantitative sequencing and immunohistochemistry revealed cortical chicken ovalbumin upstream promotor-transcription factor 1 (COUP-TFI) expression in a high ventro-posterior to low anterior gradient except for raised immunoreactivity in the anterior ventral pallium. Unlike in mouse, COUP-TFI and SP8 were extensively co-expressed in dorsal sensory neocortex and dorsal hippocampus whereas COUPTFI/COUPTFII co-expression defined ventral temporal cortex and ventral hippocampus...
October 1, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28911139/preeclampsia-downregulates-micrornas-in-fetal-endothelial-cells-roles-of-mir-29a-c-3p-in-endothelial-function
#12
Chi Zhou, Qing-Yun Zou, Hua Li, Rui-Fang Wang, Ai-Xia Liu, Ronald R Magness, Jing Zheng
Context: Preeclampsia is a leading cause of fetal and maternal morbidity and mortality during pregnancy. Although the etiology of preeclampsia is unknown, preeclampsia offspring have increased risks of developing cardiovascular disorders in adulthood, implicating that preeclampsia programs fetal vasculature in utero. Objective: We hypothesize that preeclampsia alters expression profiles of endothelial microRNAs (miRNAs) in fetal endothelial cells and disturbs the vascular endothelial growth factor A (VEGFA)- and fibroblast growth factor 2 (FGF2)-induced endothelial function...
September 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28906307/lc-i-pelvic-ring-injury-not-benign-to-the-developing-fetus
#13
John C Weinlein, Rakesh P Mashru, Edward A Perez, Sara E Johnson
OBJECTIVE: To determine if certain patterns of pelvic ring injury are associated with more frequent intra-uterine fetal demise (IUFD). DESIGN: Retrospective review SETTING:: Level 1 trauma center. PATIENTS/PARTICIPANTS: Of 44 pregnant patients with pelvic and/or acetabular fractures, 40 had complete records that allowed determination of fetal viability. Chi-square tests were used for categorical variables (Fisher's exact tests when expected cell counts were fewer than five) and t-tests were used for continuous variables...
September 11, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28900388/disc1-regulates-the-proliferation-and-migration-of-mouse-neural-stem-progenitor-cells-through-pax5-sox2-dll1-and-neurog2
#14
Qian Wu, Weiting Tang, Zhaohui Luo, Yi Li, Yi Shu, Zongwei Yue, Bo Xiao, Li Feng
Background: Disrupted-in-schizophrenia 1 (DISC1) regulates neurogenesis and is a genetic risk factor for major psychiatric disorders. However, how DISC1 dysfunction affects neurogenesis and cell cycle progression at the molecular level is still unknown. Here, we investigated the role of DISC1 in regulating proliferation, migration, cell cycle progression and apoptosis in mouse neural stem/progenitor cells (MNSPCs) in vitro. Methods: MNSPCs were isolated and cultured from mouse fetal hippocampi. Retroviral vectors or siRNAs were used to manipulate DISC1 expression in MNSPCs...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28898547/hlx-is-a-candidate-gene-for-a-pattern-of-anomalies-associated-with-congenital-diaphragmatic-hernia-short-bowel-and-asplenia
#15
Sandra A Farrell, Sandi Sodhi, Christian R Marshall, Andrea Guerin, Anne Slavotinek, Tara Paton, Karen Chong, Wilma L Sirkin, Stephen W Scherer, Félix-Antoine Bérubé-Simard, Nicolas Pilon
Isolated congenital diaphragmatic hernia is often a sporadic event with a low recurrence risk. However, underlying genetic etiologies, such as chromosome anomalies or single gene disorders, are identified in a small number of individuals. We describe two fetuses with a unique pattern of multiple congenital anomalies, including diaphragmatic hernia, short bowel and asplenia, born to first-cousin parents. Whole exome sequencing showed that both were homozygous for a missense variant, c.950A>C, predicting p...
September 12, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28898058/mechanoregulation-of-sm22%C3%AE-transgelin
#16
Rong Liu, M Moazzem Hossain, Xuequn Chen, Jian-Ping Jin
SM22α, also named transgelin, is an actin filament-associated protein in smooth muscle and fibroblasts. Three decades after its discovery, the biological function of SM22α remains under investigation. Here we report a novel finding that the expression and degradation of SM22α/transgelin are regulated by mechanical tension. Following a mass spectrometry identification of SM22α degradation in isolated and tension-unloaded mouse aorta, we developed specific monoclonal antibodies to study the regulation of SM22α in human fetal lung myofibroblast line MRC-5 and primary cultures of neonatal mouse skin fibroblasts...
September 27, 2017: Biochemistry
https://www.readbyqxmd.com/read/28891400/glycosylated-cell-surface-markers-for-the-isolation-of-human-cardiac-progenitors
#17
Asja T Moerkamp, Hau Wan Leung, Noortje A M Bax, Stephanie Holst, Kirsten Lodder, Thijs Berends, Calinda K E Dingenouts, Andre Boon Hwa Choo, Anke M Smits, Marie-Jose Goumans
The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor based cell therapy. However, the field of CPC biology suffers from the lack of human CPC-specific markers. Therefore, we raised a panel of monoclonal antibodies (mAb) against CPCs Of this panel of antibodies, we show that mAb C1096 recognizes a progenitor-like population in the fetal and adult human heart and partially co-localize with reported CPC populations in vitro...
September 11, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28862380/fetal-bone-marrow-derived-mesenchymal-stem-stromal-cells-enhance-humanization-and-bone-formation-of-bmp7-loaded-scaffolds
#18
Abbas Shafiee, Jeremy G Baldwin, Jatin Patel, Boris M Holzapfel, Nicholas M Fisk, Kiarash Khosrotehrani, Dietmar W Hutmacher
Tissue engineered constructs built with human cells capable of generating a bone-like organ within the mouse have attracted considerable interest over the past decade. Here, we aimed to compare the utility of human mesenchymal stem/stromal cells (MSC) isolated from fetal term placenta (fPL-MSC) and fetal first trimester bone marrow (fBM-MSC) in a polycaprolactone scaffold/BMP7-based model in nude mice. Furthermore, fPL-MSC were co-seeded with fetal placenta-derived endothelial colony forming cells (ECFC) to assess the impact of ECFC on fPL-MSC osteogenesis...
September 1, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28858636/damage-associated-molecular-pattern-markers-hmgb1-and-cell-free-fetal-telomere-fragments-in-oxidative-stressed-amnion-epithelial-cell-derived-exosomes
#19
Samantha Sheller-Miller, Rheanna Urrabaz-Garza, George Saade, Ramkumar Menon
Term labor in humans is associated with increased oxidative stress (OS) -induced senescence and damages to amnion epithelial cells (AECs). Senescent fetal cells release alarmin high-mobility group box 1 (HMGB1) and cell-free fetal telomere fragments (cffTF) which can be carried by exosomes to other uterine tissues to produce parturition-associated inflammatory changes. This study characterized AEC-derived exosomes under normal and OS conditions and their packaging of HMGB1 and cffTF. Primary AECs were treated with either standard media or oxidative stress-induced media (exposure to cigarette smoke extract for 48h)...
September 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/28851297/an-a%C3%AE-globin-g-a-gene-polymorphism-associated-with-%C3%AE-0-39-thalassemia-globin-gene-and-high-fetal-hemoglobin-production
#20
Giulia Breveglieri, Nicoletta Bianchi, Lucia Carmela Cosenza, Maria Rita Gamberini, Francesco Chiavilli, Cristina Zuccato, Giulia Montagner, Monica Borgatti, Ilaria Lampronti, Alessia Finotti, Roberto Gambari
BACKGROUND: Increase of the expression of γ-globin gene and high production of fetal hemoglobin (HbF) in β-thalassemia patients is widely accepted as associated with a milder or even asymptomatic disease. The search for HbF-associated polymorphisms (such as the XmnI, BCL11A and MYB polymorphisms) has recently gained great attention, in order to stratify β-thalassemia patients with respect to expectancy of the first transfusion, need for annual intake of blood, response to HbF inducers (the most studied of which is hydroxyurea)...
August 29, 2017: BMC Medical Genetics
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