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fetal cell isolation

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https://www.readbyqxmd.com/read/29661028/development-of-large-scale-manufacturing-of-adipose-derived-stromal-cells-for-clinical-applications-using-bioreactors-and-human-platelet-lysate
#1
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/29650045/characterization-of-the-interaction-between-human-decidua-parietalis-mesenchymal-stem-stromal-cells-and-natural-killer-cells
#2
M H Abumaree, E Bahattab, A Alsadoun, A Al Dosaimani, F M Abomaray, T Khatlani, B Kalionis, M F El-Muzaini, A O Alawad, A S AlAskar
BACKGROUND: Human decidua parietalis mesenchymal stem/multipotent stromal cells (DPMSCs) have unique phenotypic and functional properties that make them promising candidates for cell-based therapy. Here, we investigated DPMSC interaction with natural killer (NK) cells, and the effects of this interaction on NK cell phenotypic characteristics and functional activities. METHODS: DPMSCs isolated from the decidua parietalis of human fetal membranes were cultured with interleukin (IL)-2-activated and IL-2-unactivated NK cells isolated from healthy human peripheral blood...
April 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29626747/non-invasive-prenatal-paternity-testing-using-cell-free-fetal-dna-from-maternal-plasma-dna-isolation-and-genetic-marker-studies
#3
REVIEW
Shanshan Zhang, Shuyi Han, Maoxiu Zhang, Yunshan Wang
Invasive prenatal paternity tests can result in miscarriage and congenital malformations; therefore, a non-invasive method of testing is preferable. However, little progress could be made in this field until the introduction of cell-free fetal DNA (cffDNA) in 2009. In this review, two aspects regarding the history and development of non-invasive prenatal paternity testing (NIPAT) are summarized: (1) extraction and enrichment of cffDNA and (2) genetic marker-based studies. Although column-based kits are used widely for NIPAT, some researchers have suggested that an automated method, such as magnetic extraction, generally has a higher cffDNA yield than that of manual column-based extraction; therefore, its popularity might increase in the near future...
April 1, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29617759/regulation-of-gene-expression-by-thyroid-hormone-in-primary-astrocytes-factors-influencing-the-genomic-response
#4
Beatriz Morte, Pilar Gil-Ibáñez, Juan Bernal
Astrocytes mediate the action of thyroid hormone in the brain on other neural cells through the production of the active hormone triiodothyronine (T3) from its precursor thyroxine (T4). T3 has also many effects on the astrocytes in vivo and in culture, but whether these actions are directly mediated by transcriptional regulation is not clear. In this work we have analyzed the genomic response to T3 of cultured astrocytes isolated from the postnatal mouse cerebral cortex, using RNA sequencing. Cultured astrocytes express relevant genes of thyroid hormone metabolism and action encoding type 2 deiodinase (Dio2), Mct8 transporter (Slc16a2), T3 receptors (Thra1 and Thrb), and nuclear corepressor (Ncor1) and coactivator (Ncoa1)...
April 2, 2018: Endocrinology
https://www.readbyqxmd.com/read/29615915/application-of-mesenchymal-stem-cells-for-therapeutic-agent-delivery-in-anti-tumor-treatment
#5
REVIEW
Daria S Chulpanova, Kristina V Kitaeva, Leysan G Tazetdinova, Victoria James, Albert A Rizvanov, Valeriya V Solovyeva
Mesenchymal stem cells (MSCs) are non-hematopoietic progenitor cells, which can be isolated from different types of tissues including bone marrow, adipose tissue, tooth pulp, and placenta/umbilical cord blood. There isolation from adult tissues circumvents the ethical concerns of working with embryonic or fetal stem cells, whilst still providing cells capable of differentiating into various cell lineages, such as adipocytes, osteocytes and chondrocytes. An important feature of MSCs is the low immunogenicity due to the lack of co-stimulatory molecules expression, meaning there is no need for immunosuppression during allogenic transplantation...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29608700/trophoblast-retrieval-and-isolation-from-the-cervix-origins-of-cervical-trophoblasts-and-their-potential-value-for-risk-assessment-of-ongoing-pregnancies
#6
Gerit Moser, Sascha Drewlo, Berthold Huppertz, D Randall Armant
BACKGROUND: Early during human development, the trophoblast lineage differentiates to commence placentation. Where the placenta contacts the uterine decidua, extravillous trophoblast (EVT) cells differentiate and invade maternal tissues. EVT cells, identified by expression of HLA-G, invade into uterine blood vessels (endovascular EVT), as well as glands (endoglandular EVT), and open such luminal structures towards the intervillous space of the placenta. Endoglandular invasion diverts the contents of uterine glands to the intervillous space, while glands near the margin of the placenta that also contain endoglandular EVT cells open into the reproductive tract...
March 28, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29600283/zika-virus-infection-preferentially-counterbalances-human-peripheral-monocyte-and-or-nk-cell-activity
#7
Fok-Moon Lum, David Lee, Tze-Kwang Chua, Jeslin J L Tan, Cheryl Y P Lee, Xuan Liu, Yongxiang Fang, Bernett Lee, Wearn-Xin Yee, Natasha Y Rickett, Po-Ying Chia, Vanessa Lim, Yee-Sin Leo, David A Matthews, Julian A Hiscox, Lisa F P Ng
Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways...
March 2018: MSphere
https://www.readbyqxmd.com/read/29596530/establishment-and-characterization-of-pygmy-killer-whale-feresa-attenuata-dermal-fibroblast-cell-line
#8
Sun Yajing, Imran Rashid Rajput, Huang Ying, Yu Fei, Edmond Sanganyado, Li Ping, Wang Jingzhen, Liu Wenhua
The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate...
2018: PloS One
https://www.readbyqxmd.com/read/29594155/effect-of-a-histone-demethylase-inhibitor-on-equine-herpesvirus-1-activity-in-vitro
#9
Rebecca L Tallmadge, Emilija Žygelytė, Gerlinde R Van de Walle, Thomas M Kristie, M Julia B Felippe
Equine herpesvirus type 1 (EHV-1) is a ubiquitous and highly contagious pathogen that causes a range of disease severities with outbreaks of notable economic impact. Given the limitations in immune protection of current vaccines and the limited effectiveness of antiviral drugs on EHV-1 infections in vivo , improved treatment measures are needed to control disease. The use of drugs that alter the epigenetic state of herpes simplex virus genome has been shown to limit viral primary infection and reactivation both in vitro and in vivo ...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29587824/distinctive-epigenomes-characterize-glioma-stem-cells-and-their-response-to-differentiation-cues
#10
Dan Zhou, Bonnie M Alver, Shuang Li, Ryan A Hlady, Joyce J Thompson, Mark A Schroeder, Jeong-Heon Lee, Jingxin Qiu, Philip H Schwartz, Jann N Sarkaria, Keith D Robertson
BACKGROUND: Glioma stem cells (GSCs) are a subpopulation of stem-like cells that contribute to glioblastoma (GBM) aggressiveness, recurrence, and resistance to radiation and chemotherapy. Therapeutically targeting the GSC population may improve patient survival, but unique vulnerabilities need to be identified. RESULTS: We isolate GSCs from well-characterized GBM patient-derived xenografts (PDX), characterize their stemness properties using immunofluorescence staining, profile their epigenome including 5mC, 5hmC, 5fC/5caC, and two enhancer marks, and define their transcriptome...
March 27, 2018: Genome Biology
https://www.readbyqxmd.com/read/29579271/interleukin-34-is-present-at-the-fetal-maternal-interface-and-induces-immunoregulatory-macrophages-of-a-decidual-phenotype-in-vitro
#11
R Lindau, R B Mehta, G E Lash, G Papapavlou, R Boij, G Berg, M C Jenmalm, J Ernerudh, J Svensson-Arvelund
STUDY QUESTION: Is the newly discovered cytokine interleukin (IL)-34 expressed at the human fetal-maternal interface in order to influence polarization of monocytes into macrophages of a decidual immunoregulatory phenotype? SUMMARY ANSWER: IL-34 was found to be present at the fetal-maternal interface, in both fetal placenta and maternal decidua, and it was able to polarize monocytes into macrophages of a decidual phenotype. WHAT IS KNOWN ALREADY: IL-34 was shown to bind to the same receptor as macrophage-colony stimulating factor (M-CSF), which has an important immunomodulatory role at the fetal-maternal interface, for example by polarizing decidual macrophages to an M2-like regulatory phenotype...
April 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29568109/mitochondrial-inefficiency-in-infants-born-to-overweight-african-american-mothers
#12
Manjusha Abraham, Christina A Collins, Scott Flewelling, Maraya Camazine, Alison Cahill, W Todd Cade, Jennifer G Duncan
BACKGROUND: Currently 20-35% of pregnant women are obese, posing a major health risk for mother and fetus. It is postulated that an abnormal maternal-fetal nutritional environment leads to adverse metabolic programming, resulting in altered substrate metabolism in the offspring and predisposing to risks of obesity and diabetes later in life. Data indicate that oocytes from overweight animals have abnormal mitochondria. We hypothesized that maternal obesity is associated with altered mitochondrial function in healthy neonatal offspring...
March 3, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29563048/stem-cells-their-source-potency-and-use-in-regenerative-therapies-with-focus-on-adipose-derived-stem-cells-a-review
#13
REVIEW
Lucie Bacakova, Jana Zarubova, Martina Travnickova, Jana Musilkova, Julia Pajorova, Petr Slepicka, Nikola Slepickova Kasalkova, Vaclav Svorcik, Zdenka Kolska, Hooman Motarjemi, Martin Molitor
Stem cells can be defined as units of biological organization that are responsible for the development and the regeneration of organ and tissue systems. They are able to renew their populations and to differentiate into multiple cell lineages. Therefore, these cells have great potential in advanced tissue engineering and cell therapies. When seeded on synthetic or nature-derived scaffolds in vitro, stem cells can be differentiated towards the desired phenotype by an appropriate composition, by an appropriate architecture, and by appropriate physicochemical and mechanical properties of the scaffolds, particularly if the scaffold properties are combined with a suitable composition of cell culture media, and with suitable mechanical, electrical or magnetic stimulation...
March 18, 2018: Biotechnology Advances
https://www.readbyqxmd.com/read/29562772/in-utero-transplantation-of-placenta-derived-mesenchymal-stromal-cells-for-potential-fetal-treatment-of-hemophilia-a
#14
Priyadarsini Kumar, Kewa Gao, Chuwang Wang, Christopher Pivetti, Lee Lankford, Diana Farmer, Aijun Wang
Hemophilia A (HA) is an X-linked recessive disorder caused by mutations in the factor VIII ( FVIII) gene leading to deficient blood coagulation. The current standard of care is frequent infusions of plasma-derived FVIII or recombinant B-domain-deleted FVIII (BDD-FVIII). While this treatment is effective, many patients eventually develop FVIII inhibitors that limit the effectiveness of the infused FVIII. As a monogenic disorder, HA is an ideal target for gene or cell-based therapy. Several studies have investigated allogeneic stem cell therapy targeting in utero or postnatal treatment of HA but have not been successful in completely correcting HA...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29557201/cell-loaded-carboxymethylcellulose-microspheres-sustain-viability-and-proliferation-of-atdc5-cells
#15
Yu Ke, Caikun Liu, Yanting Wang, Meng Xiao, Jiachen Fan, Pengcheng Fu, Shuhao Wang, Gang Wu
Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular weight, high CMC-Ph concentration (>0.8 g/ml) or long culturing period had obvious inhibition effect on ATDC5 proliferation, but low horseradish peroxidase concentration (HRP, <0.4 mg/ml) did not. CMC-Ph gels being obtained through HRP/H2 O2 reaction showed an enhancing strength and decreasing break stain as the molecular weight of CMC-Ph increased, along with a decreasing gelation time...
March 20, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29545257/advanced-whole-genome-sequencing-and-analysis-of-fetal-genomes-from-amniotic-fluid
#16
Qing Mao, Robert Chin, Weiwei Xie, Yuqing Deng, Wenwei Zhang, Huixin Xu, Rebecca Yu Zhang, Quan Shi, Erin E Peters, Natali Gulbahce, Zhenyu Li, Fang Chen, Radoje Drmanac, Brock A Peters
BACKGROUND: Amniocentesis is a common procedure, the primary purpose of which is to collect cells from the fetus to allow testing for abnormal chromosomes, altered chromosomal copy number, or a small number of genes that have small single- to multibase defects. Here we demonstrate the feasibility of generating an accurate whole-genome sequence of a fetus from either the cellular or cell-free DNA (cfDNA) of an amniotic sample. METHODS: cfDNA and DNA isolated from the cell pellet of 31 amniocenteses were sequenced to approximately 50× genome coverage by use of the Complete Genomics nanoarray platform...
March 15, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29540503/a-niche-of-trophoblast-progenitor-cells-identified-by-integrin-%C3%AE-2-is-present-in-first-trimester-human-placentas
#17
Cheryl Q E Lee, Margherita Turco, Lucy Gardner, Benjamin Simons, Myriam Hemberger, Ashley Moffett
During pregnancy the trophoblast cells of the placenta are the only fetal cells in direct contact with maternal blood and decidua. Their functions include transport of nutrients and oxygen, secretion of pregnancy hormones, remodelling the uterine arteries, and communicating with maternal cells. Despite the importance of trophoblast cells in placental development and successful pregnancy, little is known about the identity, location and differentiation of human trophoblast progenitors. We identify a proliferative trophoblast niche at the base of the cytotrophoblast cell columns in first trimester placentas that is characterised by integrin α2 (ITGA2) expression...
March 14, 2018: Development
https://www.readbyqxmd.com/read/29536580/clinical-application-of-targeted-next-generation-sequencing-on-fetuses-with-congenital-heart-defects
#18
Ping Hu, Fengchang Qiao, Yan Wang, Lulu Meng, Xiuqing Ji, Chunyu Luo, Tianhui Xu, Ran Zhou, Jingjing Zhang, Bin Yu, Leilei Wang, Ting Wang, Qiong Pan, Dingyuan Ma, Dong Liang, Zhengfeng Xu
OBJECTIVES: This study aimed to determine the diagnostic yield of targeted next-generation sequencing (NGS) in prenatal diagnosis of congenital heart defects (CHDs) and for investigating the possible genetic etiology of prenatal CHD cases. METHODS: Forty-four fetuses with CHDs and normal molecular karyotypes underwent targeted NGS in this study. Fetal genomic DNA was directly extracted from amniotic fluid cells in each prenatal case. A customized targeted NGS panel containing 77 CHD-associated genes was designed to detect variants in the coding regions and the splicing sites of these genes...
March 13, 2018: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29533390/human-pluripotent-stem-cell-derived-hlc-transcriptome-data-enables-molecular-dissection-of-hepatogenesis
#19
Wasco Wruck, James Adjaye
Induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) differentiated into hepatocyte-like cells (HLCs) provide a defined and renewable source of cells for drug screening, toxicology and regenerative medicine. We previously reprogrammed human fetal foreskin fibroblast cells (HFF1) into iPSCs employing an episomal plasmid-based integration-free approach, this iPSC-line and the hESC lines H1 and H9 were used to model hepatogenesis in vitro. Biochemical characterisation confirmed glycogen storage, ICG uptake and release, urea and bile acid production, as well as CYP3A4 activity...
March 13, 2018: Scientific Data
https://www.readbyqxmd.com/read/29513649/human-hippocampal-neurogenesis-drops-sharply-in-children-to-undetectable-levels-in-adults
#20
Shawn F Sorrells, Mercedes F Paredes, Arantxa Cebrian-Silla, Kadellyn Sandoval, Dashi Qi, Kevin W Kelley, David James, Simone Mayer, Julia Chang, Kurtis I Auguste, Edward F Chang, Antonio J Gutierrez, Arnold R Kriegstein, Gary W Mathern, Michael C Oldham, Eric J Huang, Jose Manuel Garcia-Verdugo, Zhengang Yang, Arturo Alvarez-Buylla
New neurons continue to be generated in the subgranular zone of the dentate gyrus of the adult mammalian hippocampus. This process has been linked to learning and memory, stress and exercise, and is thought to be altered in neurological disease. In humans, some studies have suggested that hundreds of new neurons are added to the adult dentate gyrus every day, whereas other studies find many fewer putative new neurons. Despite these discrepancies, it is generally believed that the adult human hippocampus continues to generate new neurons...
March 15, 2018: Nature
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