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https://www.readbyqxmd.com/read/28632489/the-effects-of-platelet-gel-on-cultured-human-retinal-pigment-epithelial-cells
#1
Sahar Balagholi, Shaban Alizadeh, Abouzar Bagheri, Yashar Amizadeh, Mozhgan Rezaei Kanavi
The positive role of platelet gel (PG) in tissue regeneration is well known, however, other characteristics of PG still remain to be determined. We investigated cellular and molecular changes in cultured human retinal pigment epithelial (hRPE) cells when treated with different concentrations of PG named PG1, PG2, and PG3. hRPE cells were isolated from donor eyes of two newborn children, within 24 hours after their death. The cells were treated with three concentrations of PG for 7 days: 3 × 104/ml (PG1), 6 × 104/ml (PG2), and 9 × 104/ml (PG3)...
June 20, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28626076/cardiac-fibroblast-transcriptome-analyses-support-a-role-for-interferogenic-profibrotic-and-inflammatory-genes-in-anti-ssa-ro-associated-congenital-heart-block
#2
Robert M Clancy, Androo J Markham, Tanisha Jackson, Sara E Rasmussen, Miroslav Blumenberg, Jill P Buyon
The signature lesion of SSA/Ro autoantibody-associated congenital heart block (CHB) is fibrosis and a macrophage infiltrate, supporting an experimental focus on cues influencing the fibroblast component. The transcriptomes of human fetal cardiac fibroblasts were analyzed using two complementary approaches. Cardiac injury conditions were simulated in vitro by incubating human fetal cardiac fibroblasts with supernatants from macrophages transfected with the SSA/Ro-associated hY3. The top ten upregulated transcripts in the stimulated fibroblasts reflected a type I interferon (IFN) response (e...
June 16, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28625011/liver-epithelioid-progenitor-cells-derived-from-fetal-luxi-bovine-alleviate-liver-fibrosis
#3
Kunfu Wang, Hao Liu, Jinjuan Yang, Caiyun Ma, Zebiao Zhang, Dong Zheng, Weijun Guan
Liver epithelioid progenitor cells (LEPCs) have important roles in liver therapy because of their hepatic differentiation potency in vitro and in vivo. Despite many researches on humans, mice, and rats, equivalent progenitor cells derived from bovine are relatively rare. The purpose of our current study is to characterize bovine LEPCs, and research on the cure potency of this heteroplastic progenitor cells on mice liver fibrosis. We have used collagenase IV digesting and differential adhesion method to isolate slabstone shape, EpCAM, LGR5, NCAM1 and SOX9 positive progenitor cells from fetal Luxi bovine liver...
June 17, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28623970/localization-of-the-placental-bcrp-abcg2-transporter-to-lipid-rafts-role-for-cholesterol-in-mediating-efflux-activity
#4
John T Szilagyi, Anna M Vetrano, Jeffrey D Laskin, Lauren M Aleksunes
INTRODUCTION: The breast cancer resistance protein (BCRP/ABCG2) is an efflux transporter in the placental barrier. By transporting chemicals from the fetal to the maternal circulation, BCRP limits fetal exposure to a range of drugs, toxicants, and endobiotics such as bile acids and hormones. The purpose of the present studies was to 1) determine whether BCRP localizes to highly-ordered, cholesterol-rich lipid raft microdomains in placenta microvillous membranes, and 2) determine the impact of cholesterol on BCRP-mediated placental transport in vitro...
July 2017: Placenta
https://www.readbyqxmd.com/read/28615711/identification-and-characterization-of-a-rich-population-of-cd34-mesenchymal-stem-stromal-cells-in-human-parotid-sublingual-and-submandibular-glands
#5
Padma Priya Togarrati, Robson T Sasaki, Mohamed Abdel-Mohsen, Nuntana Dinglasan, Xutao Deng, Shivani Desai, Elaine Emmerson, Elizabeth Yee, William R Ryan, Marcelo C P da Silva, Sarah M Knox, Satish K Pillai, Marcus O Muench
Mesenchymal stem/stromal cells (MSCs) play crucial roles in maintaining tissue homeostasis during physiological turnovers and injuries. Very little is known about the phenotype, distribution and molecular nature of MSCs in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. Our results demonstrate that the cell adhesion molecule CD34 was widely expressed by the MSCs of human major SGs, namely parotid (PAG), sublingual (SLG) and submandibular (SMG) glands...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28614916/gestational-diabetes-affects-the-growth-and-functions-of-perivascular-stem-cells
#6
Borim An, Eunbi Kim, Haengseok Song, Kwon-Soo Ha, Eun-Taek Han, Won Sun Park, Tae Gyu Ahn, Se-Ran Yang, Sunghun Na, Seok-Ho Hong
Gestational diabetes mellitus (GDM), one of the common metabolic disorders of pregnancy, leads to functional alterations in various cells including stem cells as well as some abnormalities in fetal development. Perivascular stem cells (PVCs) have gained more attention in recent years, for the treatment of various diseases. However, the effect of GDM on PVC function has not been investigated. In our study, we isolated PVCs from umbilical cord of normal pregnant women and GDM patients and compared their phenotypes and function...
June 13, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28612833/variant-in-the-x-chromosome-spliceosomal-gene-gpkow-causes-male-lethal-microcephaly-with-intrauterine-growth-restriction
#7
Renée Carroll, Raman Kumar, Marie Shaw, Jennie Slee, Vera M Kalscheuer, Mark A Corbett, Jozef Gecz
Congenital microcephaly, with or without additional developmental defects, is a heterogeneous disorder resulting from impaired brain development during early fetal life. The majority of causative genetic variants identified thus far are inherited in an autosomal recessive manner and impact key cellular pathways such as mitosis, DNA damage response and repair, apoptosis and splicing. Here, we report a novel donor splice site variant in the G-patch domain and KOW motifs (GPKOW) gene (NG_021310.2:g.6126G>A, NM_015698...
June 14, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28609368/derivation-and-characterization-of-a-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line
#8
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
OBJECTIVES: The aim of this study was to identify an epithelial cell line isolated from the spontaneous differentiation of totipotent pig epiblast cells. METHODS: PICM-31 and its colony-cloned derivative cell line, PICM-31A, were established from the culture and differentiation of an epiblast mass isolated from an 8-day-old pig blastocyst. The cell lines were analyzed by transmission electron microscopy, marker gene expression, and mass spectroscopy-based proteomics...
July 2017: Pancreas
https://www.readbyqxmd.com/read/28609144/therapeutic-benefit-of-mesenchymal-stem-cells-in-pregnant-rats-with-angiotensin-receptor-agonistic-autoantibody-induced-hypertension-implications-for-immunomodulation-and-cytoprotection
#9
Dan Zhang, Lihua Fu, Leilei Wang, Lin Lin, Lihong Yu, Lijun Zhang, Tao Shang
Immunomodulation by mesenchymal stem cells (MSCs) is potentially important for maintaining peripheral tolerance. Preeclampsia may be due to maternal immune rejection of the genetically foreign fetus. This study aimed to investigate the biological function of human umbilical cord-derived mesenchymal stem cells (HU-MSCs) for the treatment of angiotensin receptor agonistic autoantibody (AT1-AA)-induced hypertension during pregnancy. HU-MSCs were isolated, cultured, and labeled in vitro. AT1-AA and HU-MSCs were administered to pregnant rats...
June 13, 2017: Hypertension in Pregnancy
https://www.readbyqxmd.com/read/28604823/the-chromosome-analysis-of-the-miscarriage-tissue-miscarried-embryo-fetal-crown-rump-length-crl-measurement-a-practical-use
#10
Silvia D'ippolito, Nicoletta Di Simone, Daniela Orteschi, Maria Grazia Pomponi, Maurizio Genuardi, Leuconoe Grazia Sisti, Roberta Castellani, Esther Diana Rossi, Giovanni Scambia, Marcella Zollino
OBJECTIVE: To investigate whether miscarried embryo/fetal crown rump length (CRL) measurement may yield a practical application for predicting a conclusive result at the cytogenetic analysis of miscarriage tissue. Our study might help in improving the cytogenetic method, the results of which may be affected by maternal cell contamination (MCC). In particular, we aimed at establishing whether the miscarried embryo/fetal CRL measurement shows accuracy in predicting the possibility of MCC and the scan cut-off value useful to this purpose and, as a result, suggest a multi-step procedure for the genetic ascertainment...
2017: PloS One
https://www.readbyqxmd.com/read/28599968/the-possible-role-of-amh-in-shortening-the-gubernacular-cord-in-testicular-descent-a-reappraisal-of-the-evidence
#11
John M Hutson, Francisco A Lopez-Marambio
BACKGROUND/AIM: Anti-Müllerian hormone (AMH), also called Müllerian inhibiting substance (MIS), is glycoprotein hormone secreted by the fetal Sertoli cells to regulate regression of the Müllerian ducts, the anlagen of the uterus, fallopian tubes, and upper vagina. After its existence was predicted in 1946 and its isolation and purification in the 1970's, a huge amount of information has been gathered on its molecular biology and function in the last 30-40years. Once thought to be a locally acting factor in the male fetus during sexual differentiation, it is now recognized as an endocrine hormone present in both sexes and with functions throughout life...
May 27, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28583065/application-of-nonsense-mediated-primer-exclusion-nope-for-preparation-of-unique-molecular-barcoded-libraries
#12
Dmitriy A Shagin, Maria A Turchaninova, Irina A Shagina, Mikhail Shugay, Andrew R Zaretsky, Olga I Zueva, Dmitriy A Bolotin, Sergey Lukyanov, Dmitriy M Chudakov
BACKGROUND: Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3'-end of unwanted amplification primer (NOPE oligo) simultaneously provides a template for its elongation. This elongation disrupts specificity of unwanted primer, preventing its further participation in PCR. The suggested approach allows to rationally manage the course of PCR reactions in order to facilitate analysis of complex DNA mixtures as well as to perform multistage PCR bypassing intermediate purification steps...
June 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28582444/selection-and-characterization-of-specific-nanobody-against-bovine-virus-diarrhea-virus-bvdv-e2-protein
#13
Tiansen Li, Meiling Huang, Hongran Xiao, Guoqi Zhang, Jinhua Ding, Peng Wu, Hui Zhang, Jinliang Sheng, Chuangfu Chen
Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into Escherichia coli TG1-competent cells, to construct an initial anti-BVDV nanobody gene library. We obtained a BVDV-E2 antigen epitope bait protein by prokaryotic expression using the nucleotide sequence of the E2 gene of the BVDV-NADL strain published in GenBank...
2017: PloS One
https://www.readbyqxmd.com/read/28578005/isolation-characterization-and-differentiation-of-multipotent-neural-progenitor-cells-from-human-cerebrospinal-fluid-in-fetal-cystic-myelomeningocele
#14
Mario Marotta, Alejandra Fernández-Martín, Marc Oria, Cesar G Fontecha, Carles Giné, Vicente Martínez-Ibáñez, Elena Carreras, Michael A Belfort, Gloria Pelizzo, Jose L Peiró
Despite benefits of prenatal in utero repair of myelomeningocele, a severe type of spina bifida aperta, many of these patients will still suffer mild to severe impairment. One potential source of stem cells for new regenerative medicine-based therapeutic approaches for spinal cord injury repair is neural progenitor cells (NPCs) in cerebrospinal fluid (CSF). To this aim, we extracted CSF from the cyst surrounding the exposed neural placode during the surgical repair of myelomeningocele in 6 fetuses (20 to 26weeks of gestation)...
May 15, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28570820/human-milk-oligosaccharides-exhibit-antimicrobial-and-anti-biofilm-properties-against-group-b-streptococcus
#15
Dorothy L Ackerman, Ryan S Doster, Jörn-Hendrik Weitkamp, David Aronoff, Jennifer A Gaddy, Steven D Townsend
Streptococcus agalactiae (Group B Streptococcus, GBS) is a Gram-positive bacterial pathogen that causes invasive infections of both children and adults. During pregnancy, GBS is a significant cause of infection of the fetal membranes (chorioamnionitis), which can lead to intra-amniotic infection, preterm birth, stillbirth, and neonatal sepsis. Recently, breastfeeding has been thought to represent a potential mode of GBS transmission from mother to newborn, which might increase the risk for late-onset sepsis...
June 1, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28566515/a-microfluidics-assay-to-study-invasion-of-human-placental-trophoblast-cells
#16
Yassen Abbas, Carolin Melati Oefner, William J Polacheck, Lucy Gardner, Lydia Farrell, Andrew Sharkey, Roger Kamm, Ashley Moffett, Michelle L Oyen
Pre-eclampsia, fetal growth restriction and stillbirth are major pregnancy disorders throughout the world. The underlying pathogenesis of these diseases is defective placentation characterized by inadequate invasion of extravillous placental trophoblast cells into the uterine arteries. How trophoblast invasion is controlled remains an unanswered question but is influenced by maternal uterine immune cells called decidual natural killer cells. Here, we describe an in vitro microfluidic invasion assay to study the migration of primary human trophoblast cells...
May 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28552604/evidence-of-in%C3%A2-vitro-preservation-of-human-nephrogenesis-at-the-single-cell-level
#17
Naomi Pode-Shakked, Rotem Gershon, Gal Tam, Dorit Omer, Yehudit Gnatek, Itamar Kanter, Sarit Oriel, Guy Katz, Orit Harari-Steinberg, Tomer Kalisky, Benjamin Dekel
During nephrogenesis, stem/progenitor cells differentiate and give rise to early nephron structures that segment to proximal and distal nephron cell types. Previously, we prospectively isolated progenitors from human fetal kidney (hFK) utilizing a combination of surface markers. However, upon culture nephron progenitors differentiated and could not be robustly maintained in vitro. Here, by culturing hFK in a modified medium used for in vitro growth of mouse nephron progenitors, and by dissection of NCAM(+)/CD133(-) progenitor cells according to EpCAM expression (NCAM(+)/CD133(-)/EpCAM(-), NCAM(+)/CD133(-)/EpCAM(dim), NCAM(+)/CD133(-)/EpCAM(bright)), we show at single-cell resolution a preservation of uninduced and induced cap mesenchyme as well as a transitioning mesenchymal-epithelial state...
May 25, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28550448/towards-xeno-free-cultures-of-human-limbal-stem-cells-for-ocular-surface-reconstruction
#18
Zala Lužnik, Claudia Breda, Vanessa Barbaro, Stefano Ferrari, Angelo Migliorati, Enzo Di Iorio, Barbara Ferrari, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Marina Bertolin
Isolated limbal epithelial stem cells (LESCs) were cultured with or without a 3T3 murine fibroblast feeder-layer (FL) in 4 different culture media on culture plates or on denuded human amniotic membrane (AM) support and fibrin gel support: (1) control medium supplemented with fetal bovine serum; (2) control medium supplemented with the synthetic serum "XerumFree™ XF205" (XF); (3) CnT-20 medium supplemented with "XerumFree™ XF205" (CnT-XF) and (4) CnT-20 medium supplemented with human AB serum (CnT-AB). The three xenogeneic media were compared to standard condition (control + FL) and parameters assessed included cell morphology, proliferative potential, number of passages, assessment of clonogenic and abortive colonies, life span, ∆Np63α expression and epithelial morphology on AM...
May 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28549829/impact-of-chlorpyrifos-on-human-villous-trophoblasts-and-chorionic-villi
#19
M E Ridano, A C Racca, J B Flores-Martin, R Fretes, C L Bandeira, L Reyna, E Bevilacqua, S Genti-Raimondi, G M Panzetta-Dutari
Placental barrier regulates maternal-fetal interchange protecting the baby from damage caused by substances found in the uterine environment or circulating in the vascular system. Organophosphate (OP) pesticides are a paramount group of environmental pollutants used in intensive agriculture for protection against diseases and pests. While many studies have reported an increased risk of pregnancy alterations in pregnant women exposed to OPs, few have analyzed the effects caused by these pesticides in the placenta...
May 23, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28542561/impaired-functional-capacity-of-fetal-endothelial-cells-in-preeclampsia
#20
Lars Brodowski, Jennifer Burlakov, Sarah Hass, Constantin von Kaisenberg, Frauke von Versen-Höynck
OBJECTIVES: Preeclampsia is one of the main contributers to maternal and fetal morbidity and mortality during pregnancy. A history of preeclampsia puts mother and offspring at an increased cardiovascular risk in later life. We hypothesized that at the time of birth functional impairments of fetal endothelial cells can be detected in pregnancies complicated by preeclampsia and that a therapeutic intervention using 1,25 (OH)2 vitamin D3 can reverse the adverse effects of preeclampsia on cell function...
2017: PloS One
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