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fetal cell isolation

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https://www.readbyqxmd.com/read/29447985/selected-terpenes-from-leaves-of-ocimum-basilicum-l-induce-hemoglobin-accumulation-in-human-k562-cells
#1
Feriotto Giordana, Marchetti Nicola, Costa Valentina, Piera Torricelli, Simone Beninati, Federico Tagliati, Carlo Mischiati
Re-expression of fetal hemoglobin (HbF) was proposed as a possible therapeutic strategy for β-haemoglobinopathies. Although several inducers of HbF were tested in clinical trials, only hydroxyurea (HU) received FDA approval. Despite it produced adequate HbF levels only in half of HU-treated SCD patients, and was ineffective at all in β-thalassemia patients, beneficial effects of this approach suggested to continue in this direction identifying further molecules capable of inducing HbF. We tested the potential of essential oil isolated from Ocimum basilicum L...
February 12, 2018: Fitoterapia
https://www.readbyqxmd.com/read/29446677/how-to-integrate-cell-free-dna-screening-with-sonographic-markers-for-aneuploidy-an-update
#2
Thomas C Winter, Nancy C Rose
OBJECTIVE: The sonologist detects a so-called "soft marker" during approximately 10% of routine second-trimester anatomy examinations and is often uncertain about what further management is appropriate. This article will specifically address the management of patients with sonographic markers for six common entities: choroid plexus cysts (CPCs), ventriculomegaly (VM), echogenic intracardiac focus (EIF), urinary tract dilation (UTD), fetal echogenic bowel (FEB), and femoral and humeral shortening...
February 15, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29439091/evaluation-of-galectin-1-and-galectin-3-as-prospective-biomarkers-in-keratoconus
#3
Frans Eberth Costa Andrade, Joyce Luciana Covre, Lisandra Ramos, Rossen Mihaylov Hazarbassanov, Myrna Serapião Dos Santos, Mauro Campos, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate the expression of β-galactoside-binding proteins galectin (Gal)-1 and Gal-3 in patients with keratoconus (KC) and postcorneal collagen cross-linking (CXL) treatment in vitro. METHODS: Tear fluid, cornea samples and conjunctival impression cytology specimens from control and KC patients were used to evaluate Gal-1 and Gal-3 expressions. Primary keratocytes were isolated by collagenase digestion from surgically removed corneas of five normal or KC human corneal buttons and cultured in Dulbecco's modified eagle medium/Ham's F12 medium supplemented with 2% fetal bovine serum...
February 8, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29438480/human-trophoblast-epithelial-mesenchymal-transition-in-abnormally-invasive-placenta
#4
Sonia DaSilva-Arnold, Stacy Zamudio, Abdulla Al-Khan, Jesus Alvarez-Perez, Ciaran Mannion, Christopher Koenig, Davlyn Luke, Anisha Perez, Margaret Petroff, Manuel Alvarez, Nicholas P Illsley
Differentiation of first trimester human placental cytotrophoblast from an anchorage-dependent epithelial phenotype into the mesenchymal-like invasive extravillous trophoblast is crucial in the development of the maternal-fetal interface. We showed previously that differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition (EMT). Here we compare the epithelial-mesenchymal characteristics of cytotrophoblast (CTB) and extravillous trophoblast (EVT) derived from normal third trimester placenta or placenta previa versus abnormally invasive placenta (AIP)...
February 9, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29434243/isolation-and-expansion-of-multipotent-progenitors-from-human-trabecular-meshwork
#5
Yuan Zhang, Subo Cai, Scheffer C G Tseng, Ying-Ting Zhu
To expand multi-potent progenitors from human trabecular meshwork (TM), we have created a new optimized method on two-dimensional (2D) followed by three-dimensional (3D) Matrigel in modified embryonic stem cell medium supplemented with 5% fetal bovine serum (MESCM + 5% FBS). The expanded TM cells were small cuboidal cells expressing TM markers such as AQP1, MGP, CHI3L1, and AnkG, embryonic stem cell (ESC) markers such as Oct4, Sox2, Nanog, and ABCG2, and neural crest (NC) markers such as p75NTR, FOXD3, Sox9, Sox10, and MSX1...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29429517/in-vitro-identification-of-a-stem-cell-population-from-canine-hair-follicle-bulge-region
#6
Raquel V G de Castro, Mariana R Tavares, Fabiana F Bressan, Naira C G Pieri, Amanda Baracho Trindade Hill, Aline F Souza, Nathan da R N Cruz, Daniele S Martins, Carlos E Ambrósio, Flávio V Meirelles, Joaquim M Garcia
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2- testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ)...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29423788/evaluation-of-extraction-methods-for-methylated-cell-free-fetal-dna-from-maternal-plasma
#7
Ji Hyae Lim, Bom Yi Lee, Jin Woo Kim, You Jung Han, Jin Hoon Chung, Min Hyoung Kim, Dong Wook Kwak, So Yeon Park, Hee Back Choi, Hyun Mee Ryu
PURPOSE: Recently, fetal placenta-specific epigenetic regions (FSERs) have been identified for quantification of cell-free fetal DNA (cff-DNA) for non-invasive prenatal testing (NIPT). The aim of this study was to evaluate the efficiencies of a column-based kit and magnetic bead-based kit for quantification of methylated FSERs from maternal plasma. METHODS: Maternal plasma was extracted from normal pregnant women within the gestational age of 10~13 weeks (n = 24)...
February 8, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29422500/the-tumour-microenvironment-creates-a-niche-for-the-self-renewal-of-tumour-promoting-macrophages-in-colon-adenoma
#8
Irene Soncin, Jianpeng Sheng, Qi Chen, Shihui Foo, Kaibo Duan, Josephine Lum, Michael Poidinger, Francesca Zolezzi, Klaus Karjalainen, Christiane Ruedl
Circulating CCR2+ monocytes are crucial for maintaining the adult tissue-resident F4/80hiMHCIIhi macrophage pool in the intestinal lamina propria. Here we show that a subpopulation of CCR2-independent F4/80hiMHCIIlow macrophages, which are the most abundant F4/80hi cells in neonates, gradually decline in number in adulthood; these macrophages likely represent the fetal contribution to F4/80hi cells. In colon adenomas of ApcMin/+ mice, F4/80hiMHCIIlow macrophages are not only preserved, but become the dominant subpopulation among tumour-resident macrophages during tumour progression...
February 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29420637/isolation-and-characterization-of-mesenchymal-stem-cells-from-human-fetus-heart
#9
Venkata Naga Srikanth Garikipati, Saurabh Pratap Singh, Yamuna Mohanram, Ashwani Kumar Gupta, Deepa Kapoor, Soniya Nityanand
BACKGROUND: Mesenchymal stem cells (MSCs) are promising cells for cardiovascular regenerative medicine. However, their potential may be limited, because of their restricted cardiovascular differentiation potential and decline in their number and functional characteristics with increasing donor age. We have previously shown that rat fetus heart harbors primitive MSCs and administration of these cells improved left ventricular (LV) function after ischemia/reperfusion injury in rats. To evaluate their potential as a new cell type for clinical cardiovascular cell therapy, we have undertaken this study on the isolation and characterization of human fetal cardiac MSCs (hfC-MSCs)...
2018: PloS One
https://www.readbyqxmd.com/read/29402326/treatment-of-gingival-defects-with-gingival-mesenchymal-stem-cells-derived-from-human-fetal-gingival-tissue-in-a-rat-model
#10
Jing Li, Shi-Qing Xu, Kai Zhang, Wen-Jian Zhang, Hong-Lin Liu, Zhen Xu, Hong Li, Jin-Ning Lou, Li-Hong Ge, Bao-Hua Xu
BACKGROUND: The study aimed to evaluate the efficacy and safety of gingival mesenchymal stem cells (GMSCs) from human fetal gingival tissue used for treating gingival defects in a rat model. METHODS: GMSCs were isolated from human fetal gingival tissue and identified by flow cytometry for nestin, Oct4, vimentin, NANOG, CD105, and CD90. The immunogenicity of GMSCs was analyzed by mixed lymphocyte reactions; the tumorigenicity of GMSCs was evaluated by xenotransplanting into nude mice...
February 5, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29402311/hepatic-stem-cells-with-self-renewal-and-liver-repopulation-potential-are-harbored-in-cdcp1-positive-subpopulations-of-human-fetal-liver-cells
#11
Ran-Ran Zhang, Yun-Wen Zheng, Bin Li, Yun-Zhong Nie, Yasuharu Ueno, Tomonori Tsuchida, Hideki Taniguchi
BACKGROUND: Mature human hepatocytes are critical in preclinical research and therapy for liver disease, but are difficult to manipulate and expand in vitro. Hepatic stem cells (HpSCs) may be an alternative source of functional hepatocytes for cell therapy and disease modeling. Since these cells play an import role in regenerative medicine, the precise characterization that determines specific markers used to isolate these cells as well as whether they contribute to liver regeneration still remain to be shown...
February 5, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29396076/-what-are-the-real-purpose-and-scope-of-screening-for-aneuploidy
#12
T Quibel, P Rozenberg
In France, the recommended method for Down syndrome screening is the first trimester combined test, the risk assessment, based on maternal age, ultrasound measurement of fetal nuchal translucency and maternal serum markers (free β-hCG and PAPP-A). The Down syndrome detection rate is 78.7% at a screen positive rate of 5%. However, the best screening test is the integrated test using a combination of first trimester combined test and second trimester quadruple test (serum α-fetoprotein, human chorionic gonadotropin, unconjugated E3, and dimeric inhibin-A) and being able to achieve a detection rate for Down syndrome of approximately 96% at a screen-positive rate of 5%...
January 27, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/29394956/establishment-of-xenogeneic-serum-free-culture-methods-for-handling-human-dental-pulp-stem-cells-using-clinically-oriented-in-vitro-and-in-vivo-conditions
#13
Mai Mochizuki, Taka Nakahara
BACKGROUND: Currently, ex-vivo handling of stem cells, including transport after harvest and therapeutic preparation, is generally done in culture media containing fetal bovine serum (FBS), which promotes cell attachment, proliferation, and differentiation. However, because of safety concerns associated with the use of FBS, including potential transmission of zoonotic agents and transplant rejection because of the incorporation of foreign proteins into the stem cells, there is a need for xenogeneic serum-free culture media for clinical handling of stem cells...
February 3, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29388134/use-of-a%C3%A2-hollow-fiber-bioreactor-to-collect-extracellular-vesicles-from-cells-in-culture
#14
Irene K Yan, Neha Shukla, David A Borrelli, Tushar Patel
Current approaches for collection of extracellular vesicles (EV) are based on classical cell culture media production. This involves collection from cells grown in flasks, and can require multiple rounds of centrifugation or filtration, followed by ultracentrifugation or density gradient centrifugation. There are several limitations of these approaches, for example, they require a large input volume, the yield and concentration is low, and the process is time consuming. Most cell cultures require the use of fetal bovine serum which contains a large amount of endogenous EV that can contaminate isolations of cell-derived EVs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29384053/viral-infections-in-pregnancy-a-focus-on-ebola-virus
#15
Nicole S Olgun
During gestation, the immune response of the placenta to viruses and other pathogens plays an important role in determining a pregnant woman's vulnerability toward infectious diseases. Located at the maternal- fetal interface, trophoblast cells serve to minimize the spread of viruses between the host and developing fetus through an intricate system of innate antiviral immune signaling. Adverse pregnancy outcomes, ranging from learning disabilities to preterm birth and fetal death, are all documented results of a viral breach in the placental barrier...
January 30, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29382068/higher-cytopathic-effects-of-a-zika-virus-brazilian-isolate-from-bahia-compared-to-a-canadian-imported-thai-strain
#16
Sergio P Alpuche-Lazcano, Craig R McCullogh, Olivier Del Corpo, Elodie Rance, Robert J Scarborough, Andrew J Mouland, Selena M Sagan, Mauro M Teixeira, Anne Gatignol
Zika virus (ZIKV) is an emerging pathogen from the Flaviviridae family. It represents a significant threat to global health due to its neurological and fetal pathogenesis (including microcephaly and congenital malformations), and its rapid dissemination across Latin America in recent years. The virus has spread from Africa to Asia, the Pacific islands and the Americas with limited knowledge about the pathogenesis associated with infection in recent years. Herein, we compared the ability of the Canadian-imported Thai strain PLCal_ZV and the Brazilian isolate HS-2015-BA-01 from Bahia to produce infectious ZIKV particles and cytopathic effects in a cell proliferation assay...
January 27, 2018: Viruses
https://www.readbyqxmd.com/read/29379691/isolation-and-culture-of-primary-adult-skin-fibroblasts-from-the-asian-elephant-elephas-maximus
#17
Puntita Siengdee, Sarisa Klinhom, Chatchote Thitaram, Korakot Nganvongpanit
Background: Primary cultures from Asian elephants (Elephas maximus) allow scientists to obtain representative cells that have conserved most of their original characteristics, function, physiology and biochemistry. This technique has thus gained significant importance as a foundation for further cellular, cell biology and molecular research. Therefore, the aim of this study was to describe conditions for the successful establishment of primary adult fibroblasts from Asian elephant carcasses...
2018: PeerJ
https://www.readbyqxmd.com/read/29378552/optimized-dna-electroporation-for-primary-human-t-cell-engineering
#18
Zhang Zhang, Shunfang Qiu, Xiaopeng Zhang, Wei Chen
BACKGROUND: Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the most popular strategies for genetic material transduction. Compared with viral-particle-mediated approaches, electro-transfection is theoretically safer, because it does not promote transgene integration into the host genome. Additionally, the simplicity and speed of the procedure increases the attractiveness of electroporation...
January 30, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29377389/modified-methylated-dna-immunoprecipitation-protocol-for-noninvasive-prenatal-diagnosis-of-down-syndrome
#19
Fatemeh Karami, Mohammad R Noori-Daloii, Kobra Omidfar, Mina Tabrizi, Seddigheh Hantooshzadeh, Ashraf Aleyasin, Maryam Daneshpour, Mohammad H Modarressi
AIM: Methylated DNA immunoprecipitation real-time quantitative polymerase chain reaction (MeDIP-real-time qPCR) has been introduced as noninvasive prenatal test that has shown absolute detection rate in the screening of Down syndrome. Herein, we aimed to propose a novel modification of MeDIP-qPCR and assess its potential to alleviate the overall cost of the test, being used in very early weeks of pregnancy, and develop it to a noninvasive prenatal diagnosis biosensor in future researches...
January 26, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29375906/fetal-ultrasound-early-diagnosis-and-natural-evolution-of-proximal-femoral-focal-deficiency
#20
Eugenio O Gerscovich, Simran Sekhon, Thomas W Loehfelm, Adam Greenspan
Proximal femoral focal deficiency is an extremely rare congenital anomaly with only a few case reports in the literature. This case illustrates one diagnosed by prenatal ultrasound. The diagnosis may be isolated or associated with other abnormalities and syndromes. This report describes the early obstetrical ultrasound diagnosis, its evolution and associated findings throughout pregnancy. To the best of our knowledge, it is the first report associating this diagnosis with sickle cell trait. Another finding in our patient was a concomitant intrauterine growth restriction that we attribute to placental infarctions and a retro placental hemorrhage, also on the basis of sickle cell trait...
December 2017: Journal of Ultrasonography
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