keyword
MENU ▼
Read by QxMD icon Read
search

C-H activation

keyword
https://www.readbyqxmd.com/read/28651382/assignment-of-sigma-factors-of-rna-polymerase-to-promoters-in-corynebacterium-glutamicum
#1
Hana Dostálová, Jiří Holátko, Tobias Busche, Lenka Rucká, Andrey Rapoport, Petr Halada, Jan Nešvera, Jörn Kalinowski, Miroslav Pátek
Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σ(A)), the primary-like σ(B) and five alternative sigma factors (σ(C), σ(D), σ(E), σ(H) and σ(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28651296/early-discharge-hospital-at-home
#2
REVIEW
Daniela C Gonçalves-Bradley, Steve Iliffe, Helen A Doll, Joanna Broad, John Gladman, Peter Langhorne, Suzanne H Richards, Sasha Shepperd
BACKGROUND: Early discharge hospital at home is a service that provides active treatment by healthcare professionals in the patient's home for a condition that otherwise would require acute hospital inpatient care. This is an update of a Cochrane review. OBJECTIVES: To determine the effectiveness and cost of managing patients with early discharge hospital at home compared with inpatient hospital care. SEARCH METHODS: We searched the following databases to 9 January 2017: the Cochrane Effective Practice and Organisation of Care Group (EPOC) register, Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, Embase, CINAHL, and EconLit...
June 26, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28651288/development-of-a-high-performance-liquid-chromatographic-method-for-asiaticoside-quantification-in-different-skin-layers-after-topical-application-of-a-centella-asiatica-extract
#3
Priscila Bianca Rodrigues da Rocha, Bruno Dos Santos Souza, Lígia Marquez Andrade, Ricardo Neves Marreto, Eliana Martins Lima, Stephânia Fleury Taveira
The topical application of Centella asiatica extract has been commonly used for many different purposes but especially for cosmetic use in the treatment of gynoid lipodystrophy. Asiaticoside, the most active component in this extract, is responsible for its therapeutic activities. However, little is known to date about asiaticoside skin penetration. Thus, an analytical method for asiaticoside quantification in different skin layers after the topical application of C. asiatica extract was developed and skin permeation studies were performed with the plant extract to apply the analytical method developed...
June 26, 2017: Planta Medica
https://www.readbyqxmd.com/read/28651109/separation-of-furostanol-saponins-by-supercritical-fluid-chromatography
#4
Jie Yang, Lingling Zhu, Yang Zhao, Yongwei Xu, Qinglong Sun, Shuchen Liu, Chao Liu, Baiping Ma
Supercritical fluid chromatography (SFC) has good separation efficiency and is suitable for separating weakly polar compounds. Furostanol saponins, as an important kind of steroidal saponins, generally have two sugar chains, which are polar and hydrophilic. The hydroxyl group at the C-22 position of furostanol saponins is active and easily reacts with lower alcohols under appropriate conditions. The separation of hydrophilic furostanol saponins was tested by SFC in this study. The effects of chromatographic conditions on the separation of the mixed furostanol saponins and their hydroxyl derivatives at the C-22 position were studied...
June 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28650956/in-vitro-and-in-vivo-effects-of-kisspeptin-antagonists-p234-p271-p354-and-p356-on-gpr54-activation
#5
C H J Albers-Wolthers, J de Gier, M Walen, P J S van Kooten, C B Lambalk, P A J Leegwater, B A J Roelen, A C Schaefers-Okkens, V P M G Rutten, R P M Millar, H S Kooistra
Kisspeptins (KPs) and their receptor (GPR54 or KiSS1R) play a key-role in regulation of the hypothalamic-pituitary-gonadal axis and are therefore interesting targets for therapeutic interventions in the field of reproductive endocrinology. As dogs show a rapid and robust LH response after the administration of KP10, they can serve as a good animal model for research concerning KP signaling. The aims of the present study were to test the antagonistic properties of KP analogs p234, p271, p354, and p356 in vitro, by determining the intracellular Ca2+ response of CHEM1 cells that stably express human GPR54, and to study the in vivo effects of these peptides on basal plasma LH concentration and the KP10-induced LH response in female dogs...
2017: PloS One
https://www.readbyqxmd.com/read/28650654/evaluation-of-a-rapid-method-for-screening-heat-stress-tolerance-using-three-korean-wheat-triticum-aestivum-l-cultivars
#6
Hai An Truong, Chan Young Jeong, Won Je Lee, Byung Cheon Lee, Namhyun Chung, Chon-Sik Kang, Young-Keun Cheong, Suk-Whan Hong, Hojoung Lee
Thermotolerance in plants is a topic of concern given the current trends in global warming. Here, we aimed to develop a rapid and reproducible screening method for selection of heat stress- tolerant wheat varieties to expedite the breeding process. We tested the robustness of the screen in three Korean wheat cultivars, 'BackJung', 'KeumKang', and 'ChoKyeong'. We showed that 4-day-old seedlings of 'KeumKang' had the highest survival rates after a 45C treatment for 20 h. Moreover, the ability to retain chlorophyll and antioxidant activity was also highest in 'KeumKang'...
June 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28650507/stabilisation-of-gold-nanoparticles-by-n-heterocyclic-thiones
#7
Leonardo C Moraes, Bertrand Lacroix, Rute C Figueiredo, Patricia Lara, Javier Rojo, Salvador Conejero
Gold nanoparticles (Au-NPs) have been prepared using N-heterocyclic thiones (NHTs) as ligand stabilisers. These Au-NPs have been shown to be very stable, even in air, and have been characterized by a combination of several techniques (TEM, HR-TEM, STEM-HAADF, EDX, DLS, elemental analysis and (1)H NMR). These nanoparticles are active in the catalytic reduction of nitroarenes to anilines.
June 26, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28650494/chemical-characteristics-of-an-ilex-kuding-tea-polysaccharide-and-its-protective-effects-against-high-fructose-induced-liver-injury-and-vascular-endothelial-dysfunction-in-mice
#8
Xichuan Zhai, Daoyuan Ren, Yiyang Luo, Yuanyuan Hu, Xingbin Yang
The present study was designed to investigate the protective effects of Ilex Kuding tea polysaccharides (IKTP) on high fructose (HF)-induced liver injury and vascular endothelial dysfunction in mice. IKTP were identified as acidic heteropolysaccharides by FT-IR and HPLC. Healthy male Kunming mice fed 20% fructose in drinking water for 8 consecutive weeks significantly displayed dyslipidemia, hepatic steatosis, oxidative stress and vascular endothelial dysfunction. However, continuous administration of IKTP at 200, 400 and 800 mg per kg bw in HF-fed mice could prevent the damage caused by HF-diets, especially at dosages of 400 and 800 mg per kg bw (p < 0...
June 26, 2017: Food & Function
https://www.readbyqxmd.com/read/28650468/mkp-1-suppresses-parp-1-degradation-to-mediate-cisplatin-resistance
#9
J Wang, D H Kho, J-Y Zhou, R J Davis, G S Wu
Understanding the mechanisms of platinum compound resistance, including cisplatin resistance, has important implications for improving cancer treatments. Previous studies identified a potential role for mitogen-activated protein kinase phosphatase-1 (MKP-1) in cisplatin resistance. This work focuses on the regulation of poly(ADP-ribose) polymerase-1 (PARP-1) expression by MKP-1. We found that MKP-1 overexpression stimulates PARP-1 and poly(ADP-ribose) (PAR) protein expression and cisplatin resistance while its downregulation suppresses PARP-1 and PAR protein expression and cisplatin resistance...
June 26, 2017: Oncogene
https://www.readbyqxmd.com/read/28650465/mcu-dependent-mitochondrial-ca-2-inhibits-nad-sirt3-sod2-pathway-to-promote-ros-production-and-metastasis-of-hcc-cells
#10
T Ren, H Zhang, J Wang, J Zhu, M Jin, Y Wu, X Guo, L Ji, Q Huang, H Zhang, H Yang, J Xing
Mitochondrial Ca(2+) signaling, which is strongly dependent on the mitochondrial Ca(2+) uniporter (MCU) complex, has a series of key roles in physiopathological processes, including energy metabolism, reactive oxygen species (ROS) production and cell apoptosis. However, a mechanistic understanding of how the mitochondrial Ca(2+) signaling is remodeled and its functional roles remains greatly limited in cancers, especially in hepatocellular carcinoma. Here we demonstrated that the MCU complex was dysregulated in hepatocellular carcinoma (HCC) cells and significantly correlated with metastasis and poor prognosis of HCC patients...
June 26, 2017: Oncogene
https://www.readbyqxmd.com/read/28649839/mechanistic-investigation-of-rh-i-catalyzed-cycloisomerization-of-benzylallene-internal-alkynes-via-c-h-activation
#11
Yasuaki Kawaguchi, Shigeo Yasuda, Chisato Mukai
Treatment of the benzylallene-internal alkynes with [RhCl(CO)2]2 effected a cycloisomerization via a Csp2-H bond activation to produce the tricyclo[9.4.0.0(3,8)]pentadecapentaene skeleton. The reaction mechanism via formation of the rhodabicyclo[4.3.0] intermediates and σ-bond metathesis between the Csp2-H bond on the benzene ring and the Csp2-Rh(III) bond was proposed. In addition, a plausible alternative mechanism for the previously reported cycloisomerization of the benzylallene-terminal alkynes could also be proposed...
June 26, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28649797/powder-catalyst-fixation-for-post-electrolysis-structural-characterisation-of-nife-layered-double-hydroxide-based-oxygen-evolution-reaction-electrocatalysts
#12
Corina Andronescu, Stefan Barwe, Edgar Ventosa, Justus Masa, Eugeniu Vasile, Bharathi Konkena, Sandra Möller, Wolfgang Schuhmann
Highly active electrocatalysts for the oxygen evolution reaction are in most cases powder nanomaterials which undergo substantial changes upon applying the high potentials required for high current density oxygen evolution. Due to the vigorous gas evolution the durability under OER conditions is disappointingly low for most powder electrocatalysts due to missing strategies to securely fix powder catalysts to electrode surfaces. Thus, reliable studies of catalysts during or after OER are often impaired. We propose composites of precursors of polybenzoxazines and organophilic modified NiFe layered double hydroxides (LDHs) to form a stable and highly conducting catalyst layer, which allows the study of the catalyst before and after electrocatalysis...
June 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28649715/mild-hydrogenation-of-amides-to-amines-over-platinum-vanadium-bimetallic-catalyst
#13
Takato Mitsudome, Kazuya Miyagawa, Zen Maeno, Tomoo Mizugaki, Koichiro Jitsukawa, Jun Yamasaki, Yasutaka Kitagawa, Kiyotomi Kaneda
Hydrogenation of amides to amines is an important reaction, but the need for high temperatures and H₂ pressures is a problem. No catalysts that are effective under mild conditions, i.e., lower than 30 bar H₂/70 °C, have yet been reported. Here, the mild hydrogenation of amides was achieved for the first time, using a Pt-V bimetallic catalyst. Amide hydrogenation under just 1 bar H₂ at 70 °C or 5 bar H₂ at room temperature was achieved using the bimetallic catalyst. The mild reaction conditions enabled highly selective hydrogenation of various amides to the corresponding amines, while inhibiting arene-hydrogenation...
June 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28649683/surface-assisted-ullmann-coupling
#14
M Lackinger
Surface-assisted Ullmann coupling is the workhorse of on-surfaces synthesis. Among the various couplings that were successfully transferred from solution to solid surfaces, Ullmann coupling is arguably the most reliable, controllable, and widespread coupling reaction. The basic reaction scheme is straightforward: halogenated precursors are deposited onto solid surfaces, normally of coinage metals. In the adsorbed state the halogen substitutents are split off by virtue of the surface's reactivity, thereby generating acitvated species that subsequently recombine by forming C-C bonds...
June 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28649682/rhodium-catalyzed-redox-neutral-coupling-of-phenidones-with-alkynes
#15
Zhoulong Fan, Heng Lu, Wei Li, Kaijun Geng, Ao Zhang
A switchable synthesis of N-substituted indole derivatives from phenidones via rhodium-catalyzed redox-neutral C-H activation has been achieved. In this protocol, we firstly disclosed that the reactivity of Rh(iii) catalysis could be enhanced through employing palladium acetate as an additive. Some representative features include external oxidant-free, applicable to terminal alkynes, short reaction time and operational simplicity. The utility of this method is further showcased by the economical synthesis of potent anticancer PARP-1 inhibitors...
June 26, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28649614/integrated-molecular-landscape-of-parkinson-s-disease
#16
C J H M Klemann, G J M Martens, M Sharma, M B Martens, O Isacson, T Gasser, J E Visser, G Poelmans
Parkinson's disease is caused by a complex interplay of genetic and environmental factors. Although a number of independent molecular pathways and processes have been associated with familial Parkinson's disease, a common mechanism underlying especially sporadic Parkinson's disease is still largely unknown. In order to gain further insight into the etiology of Parkinson's disease, we here conducted genetic network and literature analyses to integrate the top-ranked findings from thirteen published genome-wide association studies of Parkinson's disease (involving 13...
2017: NPJ Parkinson's Disease
https://www.readbyqxmd.com/read/28649063/isolation-of-phytoconstituents-and-evaluation-of-anticancer-and-antioxidant-potential-of-launaea-mucronata-forssk-muschl-subsp
#17
Eman R Elsharkawy
Traditional Medicine is widely used in clinical research due to its low toxicity, low number of side effects, and low cost. Many components of common fruits and vegetables play well-documented roles as chemo preventive or chemotherapeutic agents that suppress tumor genesis. The present study was aimed to identify the DPPH (2, 2-diphenyl-1-picrylhydrazyl) free-radical scavenging constituents from methanol extract of Launaea mucronata using bioassay-guided fractionation. The methanolic extract revealed a strong antioxidant activity, the IC50 values (the concentration with scavenging activity of 50%) was 25...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28648820/selective-erasure-of-distinct-forms-of-long-term-synaptic-plasticity-underlying-different-forms-of-memory-in-the-same-postsynaptic-neuron
#18
Jiangyuan Hu, Larissa Ferguson, Kerry Adler, Carole A Farah, Margaret H Hastings, Wayne S Sossin, Samuel Schacher
Generalization of fear responses to non-threatening stimuli is a feature of anxiety disorders. It has been challenging to target maladaptive generalized memories without affecting adaptive memories. Synapse-specific long-term plasticity underlying memory involves the targeting of plasticity-related proteins (PRPs) to activated synapses. If distinct tags and PRPs are used for different forms of plasticity, one could selectively remove distinct forms of memory. Using a stimulation paradigm in which associative long-term facilitation (LTF) occurs at one input and non-associative LTF at another input to the same postsynaptic neuron in an Aplysia sensorimotor preparation, we found that each form of LTF is reversed by inhibiting distinct isoforms of protein kinase M (PKM), putative PRPs, in the postsynaptic neuron...
June 12, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28648718/an-implantable-two-axis-micromanipulator-made-with-a-3d-printer-for-recording-neural-activity-in-free-swimming-fish
#19
Loranzie S Rogers, Jacey C Van Wert, Allen F Mensinger
BACKGROUND: Chronically implanted electrodes allow monitoring neural activity from free moving animals. While a wide variety of implanted headstages, microdrives and electrodes exist for terrestrial animals, few have been developed for use with aquatic animals. NEW METHOD: A two axis micromanipulator was fabricated with a Formlabs 3D printer for implanting electrodes into free-swimming oyster toadfish (Opsanus tau). The five piece manipulator consisted of a base, body, electrode holder, manual screw drive and locking nut...
June 22, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28648667/simultaneous-recordings-of-intrinsic-cardiac-nerve-activities-and-skin-sympathetic-nerve-activities-from-human-patients-during-the-postoperative-period
#20
Mark J Shen, Arthur C Coffey, Susan Straka, David E Adams, David B Wagner, Richard J Kovacs, Michael Clark, Changyu Shen, Lan S Chen, Thomas H Everett, Shien-Fong Lin, Peng-Sheng Chen
BACKGROUND: Intrinsic cardiac nerve activities (ICNA) and the skin nerve activities (SKNA) are both associated with cardiac arrhythmias in dogs. OBJECTIVE: To test the hypothesis that ICNA and SKNA correlate with postoperative cardiac arrhythmias in humans. METHODS: Eleven patients (60±13 years old; 4 female) were enrolled in this study. Electrical signals were simultaneously recorded from electrocardiogram (ECG) patch electrodes on the chest wall and from two temporary pacing wires placed during open heart surgery on the left atrial epicardial fat pad...
June 22, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
keyword
keyword
105563
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"