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https://www.readbyqxmd.com/read/28523221/reading-lines-within-the-cocaine-addicted-brain
#1
Glenn A Doyle, Tara T Doucet-O'Hare, Matthew J Hammond, Richard C Crist, Adam D Ewing, Thomas N Ferraro, Deborah C Mash, Haig H Kazazian, Wade H Berrettini
INTRODUCTION: Long interspersed element (LINE)-1 (L1) is a type of retrotransposon capable of mobilizing into new genomic locations. Often studied in Mendelian diseases or cancer, L1s may also cause somatic mutation in the developing central nervous system. Recent reports showed L1 transcription was activated in brains of cocaine-treated mice, and L1 retrotransposition was increased in cocaine-treated neuronal cell cultures. We hypothesized that the predisposition to cocaine addiction may result from inherited L1s or somatic L1 mobilization in the brain...
May 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28521351/the-endoscopic-endonasal-transmaxillary-approach-to-meckel-s-cave-through-the-inferior-orbital-fissure
#2
Xin Zhang, Halima Tabani, Ivan El-Sayed, Matthew Russell, Xuequan Feng, Arnau Benet
BACKGROUND: Surgical access to Meckel's Cave (MC) is challenging due to its deep location and surrounding important neurovascular structures. Currently existing endoscopic endonasal (EE) approaches require dissecting near the internal carotid artery (ICA) or require transposition of the pterygopalatine neurovascular bundle. OBJECTIVE: To describe a novel approach to access the anterolateral aspect of the MC using a minimally invasive EE route. METHODS: The EE transmaxillary transinferior orbital fissure approach was simulated in 10 specimens...
June 1, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/28516305/arsenic-toxicity-and-epimutagenecity-the-new-lineage
#3
REVIEW
Somnath Paul, Pritha Bhattacharjee, Ashok K Giri, Pritha Bhattacharjee
Global methylation pattern regulates the normal functioning of a cell. Research have shown arsenic alter these methylation landscapes within the genome leading to aberrant gene expression and inducts various pathophysiological outcomes. Long interspersed nuclear elements (LINE-1) normally remains inert due to heavy methylation of it's promoters, time and various environmental insults, they lose these methylation signatures and begin retro-transposition that has been associated with genomic instability and cancerous outcomes...
May 17, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28496070/trace-elemental-determination-using-a-portable-total-reflection-x-ray-fluorescence-spectrometer-with-a-collodion-film-sample-holder
#4
Shinsuke Kunimura, Tomoki Shinkai
Using a portable total-reflection X-ray fluorescence (TXRF) spectrometer with a collodion film sample holder, a spectrum of an analyte containing 50 ng of aluminum was measured. The Al Kα line (1.49 keV) that partially overlaps with the Si Kα line (1.74 keV) from a quartz glass substrate usually used as a sample holder for TXRF analysis, was clearly detected when using the collodion film sample holder. To investigate the quantitative performance of the portable spectrometer with a collodion film sample holder, the concentrations of Cr, Mn, and Fe in a certified reference material of river water (JSAC 0302-3b), whose certified values are 10...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28495587/dose-response-analysis-of-epigenetic-metabolic-and-apical-endpoints-after-short-term-exposure-to-experimental-hepatotoxicants
#5
Isabelle R Miousse, Lynea A Murphy, Haixia Lin, Melissa R Schisler, Jinchun Sun, Marie-Cecile G Chalbot, Radhakrishna Sura, Kamin Johnson, Matthew J LeBaron, Ilias G Kavouras, Laura K Schnackenberg, Richard D Beger, Reza J Rasoulpour, Igor Koturbash
Identification of sensitive and novel biomarkers or endpoints associated with toxicity and carcinogenesis is of a high priority. There is increasing interest in the incorporation of epigenetic and metabolic biomarkers to complement apical data; however, a number of questions, including the tissue specificity, dose-response patterns, early detection of those endpoints, and the added value need to be addressed. In this study, we investigated the dose-response relationship between apical, epigenetic, and metabolomics endpoints following short-term exposure to experimental hepatotoxicants, clofibrate (CF) and phenobarbital (PB)...
May 8, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28489894/analysis-of-global-dna-methylation-changes-in-primary-human-fibroblasts-in-the-early-phase-following-x-ray-irradiation
#6
Anna Maierhofer, Julia Flunkert, Marcus Dittrich, Tobias Müller, Detlev Schindler, Indrajit Nanda, Thomas Haaf
Epigenetic alterations may contribute to the generation of cancer cells in a multi-step process of tumorigenesis following irradiation of normal body cells. Primary human fibroblasts with intact cell cycle checkpoints were used as a model to test whether X-ray irradiation with 2 and 4 Gray induces direct epigenetic effects (within the first cell cycle) in the exposed cells. ELISA-based fluorometric assays were consistent with slightly reduced global DNA methylation and hydroxymethylation, however the observed between-group differences were usually not significant...
2017: PloS One
https://www.readbyqxmd.com/read/28487269/the-function-of-line-1-encoded-reverse-transcriptase-in-tumorigenesis
#7
Ye Zhongjie, Liu Qipeng, Cen Shan, Li Xiaoyu
The retrotransposon LINE-1 is the largest transposon family of humans with an estimated 500 000 copies representing 17% of the human genome. Meanwhile, it is also the sole autonomous transposon in humans. The reverse transcriptase encoded by LINE-1 is the key component required for its transposition process. Recent evidence suggests that the LINE-1-encoded reverse transcriptase is involved in many important physiological and pathological processes including tumorigenesis. The inhibition of LINE-1-encoded reverse transcriptase inhibits tumor progression, restores cancer cell differentiation and reprograms global transcription profiles...
May 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28484492/relationships-between-global-dna-methylation-in-circulating-white-blood-cells-and-breast-cancer-risk-factors
#8
REVIEW
Nayha Chopra-Tandon, Haotian Wu, Kathleen F Arcaro, Susan R Sturgeon
It is not yet clear whether white blood cell DNA global methylation is associated with breast cancer risk. In this review we examine the relationships between multiple breast cancer risk factors and three markers of global DNA methylation: LINE-1, 5-mdC, and Alu. A literature search was conducted using Pubmed up to April 1, 2016, using combinations of relevant outcomes such as "WBC methylation," "blood methylation," "blood LINE-1 methylation," and a comprehensive list of known and suspected breast cancer risk factors...
2017: Journal of Cancer Epidemiology
https://www.readbyqxmd.com/read/28483779/heritable-l1-retrotransposition-in-the-mouse-primordial-germline-and-early-embryo
#9
Sandra R Richardson, Patricia Gerdes, Daniel J Gerhardt, Francisco J Sanchez-Luque, Gabriela-Oana Bodea, Martin Munoz-Lopez, J Samuel Jesuadian, Marie-Jeanne H C Kempen, Patricia E Carreira, Jeffrey A Jeddeloh, Jose L Garcia-Perez, Haig H Kazazian, Adam D Ewing, Geoffrey J Faulkner
LINE-1 (L1) retrotransposons are a noted source of genetic diversity and disease in mammals. To expand its genomic footprint, L1 must mobilize in cells that will contribute their genetic material to subsequent generations. Heritable L1 insertions may therefore arise in germ cells and in pluripotent embryonic cells, prior to germline specification, yet the frequency and predominant developmental timing of such events remain unclear. Here, we applied mouse retrotransposon capture sequencing (mRC-seq) and whole-genome sequencing (WGS) to pedigrees of C57BL/6J animals, and uncovered an L1 insertion rate of ≥1 event per 8 births...
May 8, 2017: Genome Research
https://www.readbyqxmd.com/read/28470866/cho-cells-engineered-for-fluorescence-read-out-of-cell-cycle-and-growth-rate-in-real-time
#10
Grischa Fuge, Yaeseong Hong, Kristoffer Riecken, An-Ping Zeng, Uwe Jandt
For efficient production of recombinant proteins by mammalian cells in a bioreactor, optimal growth rates are required and represent the most important process parameter. We present the first successful attempt to monitor the growth behavior and cell cycle state of a mammalian production relevant cell line under bioreactor cultivation conditions up to 1.2 l, utilizing a fluorescent read-out without the need of additional staining or marking. For this purpose, we developed two new production relevant cell line derivatives (CHO-K1 FUCCI CM & CHO-K1 FUCCI CN) and corresponding analytical methods...
May 4, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28465795/the-comparison-of-modified-semilunar-technique-in-conjunction-with-connective-tissue-and-subepithelial-connective-tissue-graft-for-root-coverage-a-randomized-controlled-trial
#11
Niloofar Jenabian, Nafiseh Khanjani, Ali Bijani
BACKGROUND: Gingival recession may result in aesthetically unfavourable effects, difficulty in plaque control, increased susceptibility to root caries, and dentin hypersensitivity. OBJECTIVE: The aim of this study was to compare the use of modified semilunar techniques with connective tissue and subepithelial connective tissue grafts (Langer) for denuded root surface coverage. METHODS: In this randomized clinical trial, fourteen localized recessions of Miller class I to II were treated in 5 subjects...
February 2017: Electronic Physician
https://www.readbyqxmd.com/read/28458013/aberrant-methylation-of-runx3-is-present-in-aflatoxin-b1-induced-transformation-of-the-l02r-cell-line
#12
Shan Wang, Zhini He, Daochuan Li, Bo Zhang, Miao Li, Wenxue Li, Wei Zhu, Xiumei Xing, Xiaowen Zeng, Qing Wang, Guanghui Dong, Yongmei Xiao, Wen Chen, Liping Chen
Chronic exposure to aflatoxin B1 (AFB1) is linked to the development of hepatocellular carcinoma (HCC). To identify differentially methylated genes involved in AFB1-induced cell transformation, we analyzed DNA methylation patterns in immortal human hepatocyte L02 cells expressing an oncogenic H-Ras allele (L02R cells) and AFB1-transformed L02R (L02RT-AFB1) cells by performing genome-wide methylation profiling. We treated L02R cells with 0.3μM AFB1 weekly and observed a transformed phenotype at the 17th week post-treatment...
April 27, 2017: Toxicology
https://www.readbyqxmd.com/read/28446631/combined-vitamin-b-12-and-balanced-protein-energy-supplementation-affect-homocysteine-remethylation-in-the-methionine-cycle-in-pregnant-south-indian-women-of-low-vitamin-b-12-status
#13
Sarita Devi, Arpita Mukhopadhyay, Pratibha Dwarkanath, Tinku Thomas, Julian Crasta, Annamma Thomas, C N Sheela, Jean W Hsu, Grace J Tang, Farook Jahoor, Anura V Kurpad
Background: Low-quality dietary protein intake and vitamin B-12 deficiency could interact to decrease methionine transmethylation and remethylation rates during pregnancy and may affect epigenetic modifications of the fetal genome.Objective: The objective of this randomized, partially open-labeled intervention trial was to examine the effect of supplemental high-quality protein and vitamin B-12 on third-trimester methionine kinetics in pregnant Indian women with a low vitamin B-12 status.Methods: Pregnant women with low serum vitamin B-12 concentrations (<200 pmol/L) were randomly assigned to 1 of 3 groups: the first group received balanced protein-energy supplementation of 500 mL milk/d plus a 10-μg vitamin B-12 tablet/d (M+B-12 group; n = 30), the second group received milk (500 mL/d) plus a placebo tablet (M+P group; n = 30), and the third group received a placebo tablet alone (P group; n = 33)...
April 26, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28431148/truncated-orf1-proteins-can-suppress-line-1-retrotransposition-in-trans
#14
Mark Sokolowski, May Chynces, Dawn deHaro, Claiborne M Christian, Victoria P Belancio
Long interspersed element 1 (L1) is an autonomous non-LTR retroelement that is active in mammalian genomes. Although retrotranspositionally incompetent and functional L1 loci are present in the same genomes, it remains unknown whether non-functional L1s have any trans effect on mobilization of active elements. Using bioinformatic analysis, we identified over a thousand of human L1 loci containing at least one stop codon in their ORF1 sequence. RNAseq analysis confirmed that many of these loci are expressed...
May 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28408706/embryo-culture-in-presence-of-oviductal-fluid-induces-dna-methylation-changes-in-bovine-blastocysts
#15
Antonio Daniel Barrera, Elina Vanesa García, Meriem Hamdi, Maria-Jesús Sánchez Calabuig, Angela Patricia Lopez-Cardona, Noelia Fonseca Balvís, Dimitrios Rizos, Alfonso Gutierrez-Adan
During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1...
April 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28403857/hypermethylation-of-cdkn2a-exon-2-in-tumor-tumor-adjacent-and-tumor-distant-tissues-from-breast-cancer-patients
#16
Melanie Spitzwieser, Elisabeth Entfellner, Bettina Werner, Walter Pulverer, Georg Pfeiler, Stefan Hacker, Margit Cichna-Markl
BACKGROUND: Breast carcinogenesis is a multistep process involving genetic and epigenetic changes. Tumor tissues are frequently characterized by gene-specific hypermethylation and global DNA hypomethylation. Aberrant DNA methylation levels have, however, not only been found in tumors, but also in tumor-surrounding tissue appearing histologically normal. This phenomenon is called field cancerization. Knowledge of the existence of a cancer field and its spread are of clinical relevance...
April 12, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28390408/a-comprehensive-hybridization-model-allows-whole-herv-transcriptome-profiling-using-high-density-microarray
#17
Jérémie Becker, Philippe Pérot, Valérie Cheynet, Guy Oriol, Nathalie Mugnier, Marine Mommert, Olivier Tabone, Julien Textoris, Jean-Baptiste Veyrieras, François Mallet
BACKGROUND: Human endogenous retroviruses (HERVs) have received much attention for their implications in the etiology of many human diseases and their profound effect on evolution. Notably, recent studies have highlighted associations between HERVs expression and cancers (Yu et al., Int J Mol Med 32, 2013), autoimmunity (Balada et al., Int Rev Immunol 29:351-370, 2010) and neurological (Christensen, J Neuroimmune Pharmacol 5:326-335, 2010) conditions. Their repetitive nature makes their study particularly challenging, where expression studies have largely focused on individual loci (De Parseval et al...
April 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28389878/the-effects-of-different-hinge-positions-on-posterior-tibial-slope-in-medial-open-wedge-high-tibial-osteotomy
#18
Ho-Seung Jo, Jin-Sung Park, June-Ho Byun, Young-Bok Lee, Young-Lac Choi, Seong-Hee Cho, Dong-Kyu Moon, Sang-Hyuk Lee, Sun-Chul Hwang
PURPOSE: The purpose of this study was to determine the standard hinge position to minimize effects from medial open-wedge high tibial osteotomy (HTO) on the posterior tibial slope. METHODS: Sixteen cadaveric knees underwent medial open-wedge osteotomy using either the standard or the low hinge position. To define the standard hinge position, a line 3 cm inferior to the medial tibial plateau towards the fibular head and located its intersection with a longitudinal line 1 cm medial to the fibular shaft was drawn...
April 7, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28377395/integrated-approach-of-nutritional-and-molecular-epidemiology-mineralogical-and-chemical-pollutant-characterisation-the-protocol-of-a-cross-sectional-study-in-women
#19
Martina Barchitta, Annalisa Quattrocchi, Andrea Maugeri, Germana Barone, Paolo Mazzoleni, Alfio Catalfo, Guido De Guidi, Maria Iemmolo, Nunzio Crimi, Antonella Agodi
INTRODUCTION: Environmentally-related health and disease are the result of the exposome, the totality of a person's environmental exposures, from all sources and routes, across their lifespan. Epigenetic phenomena, including DNA methylation, can be potentially modified by environmental and lifestyle factors, and result in environmental reprogramming of the genome for exposed individuals and for future generations of offspring. OBJECTIVE: The objective of the project is to evaluate the risk of DNA hypomethylation due to air pollution, Mediterranean diet adherence, folate intake, and demographic and socioeconomic factors, in healthy women living in the metropolitan area of Catania, Italy...
April 4, 2017: BMJ Open
https://www.readbyqxmd.com/read/28360946/associations-of-lep-crh-icam-1-and-line-1-methylation-measured-in-saliva-with-waist-circumference-body-mass-index-and-percent-body-fat-in-mid-childhood
#20
Jocelyn Dunstan, Joseph P Bressler, Timothy H Moran, Jonathan S Pollak, Annemarie G Hirsch, Lisa Bailey-Davis, Thomas A Glass, Brian S Schwartz
BACKGROUND: Genetics explains a small proportion of variance in body mass index at the population level. Epigenetics, commonly measured by gene methylation, holds promise for understanding obesity risk factors and mechanisms. METHODS: Participants were 431 adolescents aged 10-15 years. BMI z-score, waist circumference z-score, and percent body fat were measured. Saliva samples were collected and methylation of promoter regions of four candidate genes or sequences (LEP, ICAM-1, CRH, and LINE-1) were measured in 3-4 CpG sites each...
2017: Clinical Epigenetics
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