Yaqiang Hong, Luyao Bie, Tao Zhang, Xiaohan Yan, Guangpu Jin, Zhuo Chen, Yang Wang, Xiufeng Li, Gaofeng Pei, Yongyan Zhang, Yantao Hong, Liang Gong, Pilong Li, Wei Xie, Yanfen Zhu, Xiaohua Shen, Nian Liu
Long interspersed element-1 (LINE-1 or L1) comprises 17% of the human genome, continuously generates genetic variations, and causes disease in certain cases. However, the regulation and function of L1 remain poorly understood. Here, we uncover that L1 can enrich RNA polymerase IIs (RNA Pol IIs), express L1 chimeric transcripts, and create contact domain boundaries in human cells. This impact of L1 is restricted by a nuclear matrix protein scaffold attachment factor B (SAFB) that recognizes transcriptionally active L1s by binding L1 transcripts to inhibit RNA Pol II enrichment...
April 2, 2024: Molecular Cell