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https://www.readbyqxmd.com/read/29917314/preparation-and-application-of-magnetic-molecularly-imprinted-polymers-for-the-isolation-of-chelerythrine-from-macleaya-cordata
#1
Ming Zhong, Yan-Hong Wang, Lu Wang, Rui-Qing Long, Chun-Lin Chen
A novel type of magnetic molecularly imprinted polymers was prepared for the selective enrichment and isolation of chelerythrine from Macleaya cordata (Willd) R. Br.. The magnetic molecularly imprinted polymers were prepared using functional Fe3 O4 @SiO2 as a magnetic support, chelerythrine as template, methacrylic acid as functional monomer, and ethylene glycol dimethacrylate as cross-linker. Density functional theory at the B3LYP/6-31G (d, p) level with Gaussian 09 software was applied to calculate the interaction energies of chelerythrine, methacrylic acid and the complexes formed from chelerythrine and methacrylic acid in different ratios...
June 19, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29917296/-experiences-of-the-burden-of-treatment-patient-reports-of-facilitated-subcutaneous-immunoglobulin-treatment-in-adults-with-immunodeficiency
#2
Christina Petersson, Ramona Fust, Carina Hagstedt, Per Vågström, Åsa Nilsdotter-Augustinsson
AIMS AND OBJECTIVES: To evaluate patient-reported experiences of facilitated subcutaneous immunoglobulin treatment in adults with primary or secondary immunodeficiency. BACKGROUND: Decreased levels of circulating antibodies (humoral immunodeficiency) are often associated with higher infection rates which cause problems in daily living, for example symptoms of severe and recurrent bacterial infections that may cause chronic lung diseases. For some diagnoses, treatment with immunoglobulin becomes critical and life-long...
June 19, 2018: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29917291/exploring-the-role-of-striatal-d1-and-d2-medium-spiny-neurons-in-action-selection-using-a-virtual-robotic-framework
#3
Jyotika Bahuguna, Philipp Weidel, Abigail Morrison
The basal ganglia have been hypothesized to be involved in action selection, i.e. resolving competition between simultaneously activated motor programs. It has been shown that the direct pathway facilitates action execution whereas the indirect pathway inhibits it. However, as the pathways are both active during an action, it remains unclear whether their role is co-operative or competitive. In order to investigate this issue, we designed a striatal model consisting of D1 and D2 medium spiny neurons (MSNs) and interfaced it to a simulated robot moving in an environment...
June 19, 2018: European Journal of Neuroscience
https://www.readbyqxmd.com/read/29917277/mechanism-of-mechanochemical-c-h-bond-activation-in-an-azobenzene-substrate-by-pd-ii-catalysts
#4
Manda Curic, Marina Juribašić Kulcar, Alen Bjelopetrović, Stipe Lukin, Ivan Halasz, Krunoslav Užarević, Ivica Đilović, Dajana Barišić, Ana Budimir
Mechanism of C-H bond activation by various Pd(II) catalysts under milling conditions has been studied by in situ Raman spectroscopy. Common Pd(II) precursors, i.e. PdCl₂, [Pd(OAc)₂]₃, PdCl₂(MeCN)₂ and [Pd(MeCN)₄][BF₄]₂, have been employed for the activation of one or two C-H bonds in an unsymmetrical azobenzene substrate. The C-H activation was achieved by all used Pd(II) precursors and their reactivity increases in the order [Pd(OAc)₂]₂ < PdCl₂(MeCN)₂ < PdCl₂ < [Pd(MeCN)₂][BF₄]₂...
June 19, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29917268/kap1-inhibits-the-raf-mek-erk-pathway-to-promote-tumorigenesis-in-a549-lung-cancer-cells
#5
Guo-Jin Wu, Jun Pen, Ying Huang, Su An, Ying Liu, Yang Yang, Qian Hao, Xiao-Xi Guo, Tian-Rui Xu
Aberrant activation of the Raf-MEK-ERK pathway has frequently been associated with various cancers, especially lung cancer. However, the key regulators of this pathway are largely unknown. Using functional proteomics screening, we found that KAP1 interacts with c-Raf. Knocking out KAP1 decreased c-Raf phosphorylation at serine 259 and increased its phosphorylation at serine 338, which activated MEK and ERK. We detected higher KAP1 expression in lung cancer tissues than in normal peri-tumoral tissues. KAP1 knockdown arrested A549 lung cancer cells in the G0/G1 phase of the cell cycle and attenuated cell growth, metastasis, the epithelial-mesenchymal transition, angiogenesis, stemness, and colony formation...
June 19, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29917223/shifts-to-earlier-selfing-in-sympatry-may-reduce-costs-of-pollinator-sharing
#6
April M Randle, Rachel B Spigler, Susan Kalisz
Coexisting plant congeners often experience strong competition for resources. Competition for pollinators can result in direct fitness costs via reduced seed set or indirect costs via heterospecific pollen transfer (HPT), causing subsequent gamete loss and unfit hybrid offspring production. Autonomous selfing may alleviate these costs, but to preempt HPT, selfing should occur early, before opportunities for HPT occur (i.e. "preemptive selfing hypothesis"). We evaluated conditions for this hypothesis in Collinsia sister species, C...
June 19, 2018: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/29917198/an-analysis-of-cognitive-functioning-of-children-and-youth-with-type-1-diabetes-t1dm-in-the-context-of-glycemic-control
#7
M Stanisławska-Kubiak, E Mojs, R W Wójciak, B Piasecki, M Matecka, J Sokalski, P Kopczyński, P Fichna
OBJECTIVE: The aim of the study was to evaluate the cognitive functioning of children and youth with type 1 diabetes (T1DM). PATIENTS AND METHODS: The study included 68 children with type 1 diabetes, aged 6-17 years, divided into 3 groups according to the level of glycated hemoglobin (HbA1c): group 1: HbA1c ≤ 6.0-7.5%; group 2: HbA1c 7.6-8.5%; group 3: HbA1c over 8.6%. Wechsler's intelligence scale (WISC-R), the Trail of 10 words and Brickenkamp's and Zillmer's d2 Test of Attention were used to assess cognitive functioning...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917179/mir-374b-promotes-osteogenic-differentiation-of-mscs-by-degrading-pten-and-promoting-fracture-healing
#8
J-B Ge, J-T Lin, H-Y Hong, Y-J Sun, Y Li, C-M Zhang
OBJECTIVE: To investigate whether miR-374b can promote the differentiation of MSCs into osteoblasts by mediating PTEN, thus promoting fracture healing. MATERIALS AND METHODS: Primary cultured mouse mesenchymal stem cells were obtained for following experiments. Flow cytometry was used to determine the expression of MSCs surface antigens to identify the purity. Alizarin red staining was used to detect whether MSCs could differentiate into osteoblasts. QRT-PCR was used to detect the expression of osteogenic marker genes as well as miR-374b and PTEN in bone marrow-derived MSCs from fractured mice model...
June 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29917172/-ten-years-with-the-who-checklist-for-safe-surgery-a-new-swedish-version-is-soon-to-be-introduced
#9
Pelle Gustafson, Anders Salomonsson, Per Svensson
The WHO Checklist for Safe Surgery has been in use in Swedish healthcare since 2009. Based on national and international experiences, we have developed the original checklist, with the aim to keep its strengths and remove the weaknesses. The most obvious difference is that each section of the original checklist has been split into a pure checklist and corresponding instructions. It is now also more obvious that the basis for the checklist is local guidelines/routines. It is primarily via these local guidelines/routines that local adaptations can be made...
June 19, 2018: Läkartidningen
https://www.readbyqxmd.com/read/29917170/-safe-surgery-saves-lives-10-years-of-swedish-experience
#10
Jon Ahlberg, Karin Pukk-Härenstam
In June 2008, WHO launched the Safe Surgery Checklist. Nine months later a Swedish translation and adaptation was launched and distributed to virtually all Swedish hospitals. It gained rapid acceptance; in the fall of 2009, 73 hospitals reported that the checklist had been implemented and that its use was part of standard routine. A questionnaire in 2010 revealed that this was true for many but not all hospitals. International studies have shown that proper use of the checklist has positive effects on postoperative morbidity and mortality...
June 19, 2018: Läkartidningen
https://www.readbyqxmd.com/read/29917168/evaluation-of-anticancer-agents-using-patient-derived-tumor-organoids-characteristically-similar-to-source-tissues
#11
Hirosumi Tamura, Arisa Higa, Hirotaka Hoshi, Gen Hiyama, Nobuhiko Takahashi, Masae Ryufuku, Gaku Morisawa, Yuka Yanagisawa, Emi Ito, Jun-Ichi Imai, Yuu Dobashi, Kiyoaki Katahira, Shu Soeda, Takafumi Watanabe, Keiya Fujimori, Shinya Watanabe, Motoki Takagi
Patient-derived tumor xenograft models represent a promising preclinical cancer model that better replicates disease, compared with traditional cell culture; however, their use is low-throughput and costly. To overcome this limitation, patient-derived tumor organoids (PDOs) were established from human lung, ovarian and uterine tumor tissues, among others, to accurately and efficiently recapitulate the tissue architecture and function. PDOs were able to be cultured for >6 months, and formed cell clusters with similar morphologies to their source tumors...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917166/sb225002-inhibits-prostate-cancer-invasion-and-attenuates-the-expression-of-bsp-opn-and-mmp%C3%A2-2
#12
Meng Xu, Huamao Jaing, Haiguang Wang, Jiajie Liu, Baohao Liu, Zhongqiang Guo
The mechanisms of malignant cell metastasis to secondary sites are complex and multifactorial. Studies have demonstrated that small integrin‑binding ligand N‑linked glycoproteins (SIBLINGs), particularly bone sialoprotein (BSP) and osteopontin (OPN), are involved in neoplastic growth and metastasis. SIBLINGs promote malignant cell invasion and metastasis by enhancing matrix metalloproteinase 2 (MMP‑2) and MMP‑9 expression. Moreover, BSP and OPN can combine with integrin, which is located on the tumor cell surface, to further promote the malignant behavior of tumor cells...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917164/long-non-coding-rna-bancr-regulates-cancer-stem-cell-markers-in-papillary-thyroid-cancer-via-the-raf-mek-erk-signaling-pathway
#13
Yuanyuan Wang, Xiangde Lin, Xinghao Fu, Wei Yan, Fusheng Lin, Penghao Kuang, Yezhe Luo, Ende Lin, Xiaoquan Hong, Guoyang Wu
Thyroid cancer is one of the most common malignant tumors of the endocrine system. Among all thyroid cancers, papillary thyroid carcinoma (PTC) is the most common type. The BRAF-activated non-coding RNA (BANCR) is a 693-bp nucleotide transcript which was first identified in melanoma. However, the role of BANCR in the development of thyroid cancer remains unclear. Therefore, the present study investigated the potential involvement of BANCR in the development of thyroid cancer in vitro using patient tissue samples and a panel of thyroid cancer cell lines, and in vivo using a xenograft mouse model...
June 18, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29917149/transitions-in-dna-polymerase-%C3%AE-%C3%AE-s-ms-dynamics-related-to-substrate-binding-and-catalysis
#14
Eugene F DeRose, Thomas W Kirby, Geoffrey A Mueller, William A Beard, Samuel H Wilson, Robert E London
DNA polymerase β (pol β) plays a central role in the DNA base excision repair pathway and also serves as an important model polymerase. Dynamic characterization of pol β from methyl-TROSY 13C-1H multiple quantum CPMG relaxation dispersion experiments of Ile and Met sidechains and previous backbone relaxation dispersion measurements, reveals transitions in μs-ms dynamics in response to highly variable substrates. Recognition of a 1-nt-gapped DNA substrate is accompanied by significant backbone and sidechain motion in the lyase domain and the DNA binding subdomain of the polymerase domain, that may help to facilitate binding of the apoenzyme to the segments of the DNA upstream and downstream from the gap...
June 18, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29917123/mental-health-nurses-encounters-with-occupational-health-services
#15
J Oates, J Jones, N Drey
Background: Staff well-being is vital to the functioning of the UK National Health Service (NHS). Mental health nurses (MHNs) with personal experience of mental illness can offer a professionally and personally informed insight into the occupational health (OH) service offered by their employer. Aims: To investigate MHNs' views of OH provision in the NHS, based on their personal experience. Methods: A qualitative interview study using a purposive sample of MHNs with personal experience of mental illness...
June 16, 2018: Occupational Medicine
https://www.readbyqxmd.com/read/29917116/pediatric-supratentorial-ependymoma-surgical-clinical-and-molecular-analysis
#16
Jock C Lillard, Garrett T Venable, Nickalus R Khan, Ruth G Tatevossian, James Dalton, Brandy N Vaughn, Paul Klimo
BACKGROUND: Pediatric supratentorial ependymomas (SEs) have distinct molecular and behavioral differences from their infratentorial counterparts. OBJECTIVE: To present our experience with pediatric SEs over a 24-yr period. METHODS: Clinical, operative, and radiographic information was abstracted retrospectively. Our primary outcomes were progression-free survival (PFS) and overall survival (OS). Detection of C11orf95-RELA rearrangement was performed using interphase fluorescence in situ hybridization (iFISH)...
June 16, 2018: Neurosurgery
https://www.readbyqxmd.com/read/29917108/hepatocyte-growth-factor-acts-as-a-mitogen-for-equine-satellite-cells-via-protein-kinase-c-%C3%AE-directed-signaling
#17
Amanda M Brandt, Joanna M Kania, Madison L Gonzalez, Sally E Johnson
Hepatocyte growth factor (HGF) signals mediate mouse skeletal muscle stem cell, or satellite cell (SC), reentry into the cell cycle and myoblast proliferation. Because the athletic horse experiences exercise-induced muscle damage, the objective of the experiment was to determine the effect of HGF on equine SC (eqSC) bioactivity. Fresh isolates of adult eqSC were incubated with increasing concentrations of HGF and the initial time to DNA synthesis was measured. Media supplementation with HGF did not shorten (P > 0...
June 16, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29917102/performance-enhancing-technologies-for-steers-grazing-tall-fescue-pastures-with-varying-levels-of-toxicity
#18
Jose M Diaz, M Shane Gadberry, Paul A Beck, John T Richeson, G Douglas Hufstedler, Don S Hubbell, John D Tucker, Tom Hess, Ky G Pohler
The objective of this study was to evaluate a combination of best management practices strategy for steer calves grazing tall fescue pastures with a range of toxicity. The experiment was conducted over two grazing seasons (fall 2015 for 91 days and spring 2016 for 84 days). Steers (n = 80 within season, BW = 197.0 ± 15.43 kg [fall] and 116.9 ± 4.88 [spring]) were stocked at 2.45 and 4.1 calves/hectare in fall and spring, respectively, to 16 pastures with varying levels of toxicity based on interim ergovaline (EV) concentration within season...
June 16, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29917096/craving-to-quit-a-randomized-controlled-trial-of-smartphone-app-based-mindfulness-training-for-smoking-cessation
#19
Kathleen A Garrison, Prasanta Pal, Stephanie S O'Malley, Brian P Pittman, Ralitza Gueorguieva, Rahil Rojiani, Dustin Scheinost, Jesse Dallery, Judson A Brewer
Introduction: Mindfulness training may reduce smoking rates and lessen the association between craving and smoking. This trial tested the efficacy of mindfulness training via smartphone app to reduce smoking. Experience sampling was used to measure real time craving, smoking and mindfulness. Methods: A researcher-blind, parallel randomized controlled trial compared the efficacy of mobile mindfulness training with experience sampling (MMT-ES; Craving to Quit) vs...
June 18, 2018: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/29917093/noninvasive-thermal-evaluation-of-ventriculoperitoneal-shunt-patency-and-cerebrospinal-fluid-flow-using-a-flow-enhancing-device
#20
Mustafa Q Hameed, David Zurakowski, Mark R Proctor, Scellig S D Stone, Benjamin C Warf, Edward R Smith, Liliana C Goumnerova, Marek Swoboda, Tomer Anor, Joseph R Madsen
BACKGROUND: While a noninvasive flow determination would be desirable in the diagnosis of cerebrospinal fluid shunt malfunction, existing studies have not yet defined a role for thermal flow detection. OBJECTIVE: To evaluate a revised test protocol using a micropumper designed to transiently enhance flow during thermal testing to determine whether thermal detection of flow is associated with progression to shunt revision surgery. METHODS: Eighty-two unique tests were performed in 71 shunts...
June 16, 2018: Neurosurgery
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