keyword
MENU ▼
Read by QxMD icon Read
search

Experiment

keyword
https://www.readbyqxmd.com/read/28724261/development-of-a-sandwich-enzyme-linked-immunosorbent-assay-for-dtmp-gh-fusion-protein-by-rational-immunogen-selection
#1
Song Wang, Mingqiang Shen, Shilei Chen, Cheng Wang, Fang Chen, Mo Chen, Gaomei Zhao, Xinze Ran, Tianmin Cheng, Yongping Su, Yang Xu, Junping Wang
dTMP-GH is a chimeric protein containing a tandem dimer of thrombopoietin mimetic peptide (dTMP) fused to human growth hormone (hGH) prepared previously by our team. It shows significant bioactivity in promoting thrombocytopoiesis, but detection of intact dTMP-GH in plasma is still a challenge due to the presence of endogenous hGH. In this study, a rabbit polyclonal antibody with high affinity to dTMP was obtained with a BSA-conjugated immunogen composed of 20 amino acids sequence spanning two TMP and the linker...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28724184/treatment-of-gestational-diabetes-mellitus-with-myo-inositol-analyzing-the-cutting-edge-starting-from-a-peculiar-case
#2
L Costabile, V Unfer
OBJECTIVE: The robust data about myo-inositol (Myo-Ins) safety profile and effectiveness opened a new scenario for the treatment and prevention of Gestational Diabetes Mellitus (GDM). We report our experience about a case of GDM successfully treated with Myo-Ins. PATIENTS AND METHODS: An overweight 29-year-old Caucasian pregnant woman, nulliparous, affected by GDM, according to the National Institute for Health and Care Excellence (NICE) Guideline. After diagnosis, the patient underwent regular glycemia checks: mean fasting blood glucose value was 103...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28724167/in-vitro-controlled-release-of-two-new-tuberculocidal-adamantane-aminoethers-from-solid-pharmaceutical-formulations-ii
#3
Marilena Vlachou, Angeliki Siamidi, Dimitrios Spaneas, Dimitrios Lentzos, Polixeni Ladia, Konstantina Anastasiou, Ioannis Papanastasiou, Angeliki-Sofia Foscolos, Markos-Orestis Georgiadis, Vangelis Karalis, Tahsin Kellici, Thomas Mavromoustakos
The aim of the present investigation was to develop matrix tablet formulations for the in vitro controlled release of two new tuberculocidal adamantane aminoethers (compounds III and IV), congeneric to the adamantane derivative SQ109, which is in final clinical trials, and aminoethers (I) and (II), using carefully selected excipients, such as polyvinylpyrrolidone, sodium alginate and lactose. The tablets were prepared using the direct compression method and dissolution experiments were conducted using the US Pharmacopoeia type II apparatus (paddle method) in gastric and intestinal fluids...
July 19, 2017: Drug Research
https://www.readbyqxmd.com/read/28724154/mapping-residency-global-health-experiences-to-the-acgme-family-medicine-milestones
#4
Maureen O Grissom, Tochi Iroku-Malize, Rita Peila, Marco Perez, Neubert Philippe
BACKGROUND AND OBJECTIVES: Global health (GH) experiences are a unique part of family medicine (FM) training that offer an opportunity for residents to demonstrate development across a multitude of the milestones recently implemented by the Accreditation Council for Graduate Medical Education (ACGME). The GH experience presents an opportunity for resident development, and including a component of written reflection can provide tangible evidence of development in areas that can be difficult to assess...
July 2017: Family Medicine
https://www.readbyqxmd.com/read/28724153/actual-and-standardized-patient-evaluations-of-medical-students-skills
#5
Amelia L Sattler, Sylvia Bereknyei Merrell, Steven Y Lin, Erika Schillinger
BACKGROUND AND OBJECTIVES: Physicians must communicate effectively with patients. Actual patients (APs) rarely evaluate medical students' clinical skills; instead, standardized patients (SPs) provide proxy ratings. It is unclear how well SP ratings mirror AP experiences. The aim of this study was to compare AP and SP assessments of medical students' communication skills and professionalism. We hypothesized that their perspectives would be similar, but distinct, and offer insight about how to more reliably include the patient's voice in medical education...
July 2017: Family Medicine
https://www.readbyqxmd.com/read/28724147/community-and-interns-perspectives-on-community-participatory-medical-education-from-passive-to-active-participation
#6
Akiteru Takamura, Hidekazu Misaki, Yousuke Takemura
BACKGROUND AND OBJECTIVES: The use of community-based medical education as a method of learning primary care is now common worldwide. However, in many cases community participation remains passive. This study sought to explore the effects of introducing community members into medical education as active teachers. Medical education taught directly by community members might be a key to comprehensive community-based learning. METHODS: This study was conducted in Japan at two postgraduate programs in community hospitals...
July 2017: Family Medicine
https://www.readbyqxmd.com/read/28724133/a-nationwide-analysis-of-cost-variation-for-autologous-free-flap-breast-reconstruction
#7
Jessica I Billig, Yiwen Lu, Adeyiza O Momoh, Kevin C Chung
Importance: Cost variation among hospitals has been demonstrated for surgical procedures. Uncovering these differences has helped guide measures taken to reduce health care spending. To date, the fiscal consequence of hospital variation for autologous free flap breast reconstruction is unknown. Objective: To investigate factors that influence cost variation for autologous free flap breast reconstruction. Design, Setting, and Participants: A secondary cross-sectional analysis was performed using the Healthcare Cost and Utilization Project National Inpatient Sample database from 2008 to 2010...
July 19, 2017: JAMA Surgery
https://www.readbyqxmd.com/read/28724129/association-of-cost-sharing-with-mental-health-care-use-involuntary-commitment-and-acute-care
#8
Bastian Ravesteijn, Eli B Schachar, Aartjan T F Beekman, Richard T J M Janssen, Patrick P T Jeurissen
Importance: A higher out-of-pocket price for mental health care may lead not only to cost savings but also to negative downstream consequences. Objective: To examine the association of higher patient cost sharing with mental health care use and downstream effects, such as involuntary commitment and acute mental health care use. Design, Setting, and Participants: This difference-in-differences study compared changes in mental health care use by adults, who experienced an increase in cost sharing, with changes in youths, who did not experience the increase and thus formed a control group...
July 19, 2017: JAMA Psychiatry
https://www.readbyqxmd.com/read/28724099/use-of-%C3%AE-15-n-and-%C3%AE-18-o-values-for-nitrate-source-identification-under-irrigated-crops-a-cautionary-vadose-zone-tale
#9
Shawn E Loo, M Cathryn Ryan, Bernie J Zebarth, Shawn H Kuchta, Denise Neilsen, Bernhard Mayer
Source nitrogen (N) identification of leachate or groundwater nitrate is complicated by N source mixing and N and oxygen (O) isotope fractionation caused by microbial N transformations. This experiment examined the δN and δO values in leachate collected over 1 yr at 55 cm below raspberry ( L.) plots receiving either synthetic fertilizer (FT) or poultry manure (MT). The large ranges of δN (FT: -2.4 to +8.7‰, MT: +1.6 to +9.6‰) and δO (FT: -9.9 to -0.3‰, MT: -10.9 to +1.7‰) values in leachate collected under crop rows prohibited the reliable identification of the applied N sources on individual sampling dates...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28724093/persistence-of-ascaris-spp-ova-in-tropical-soil-cultivated-with-eucalyptus-and-fertilized-with-municipal-biosolids
#10
Marianne F de Faria, Iraê A Guerrini, Fernando C Oliveira, Maria Inês Z Sato, Elayse M Hachich, José Raimundo S Passos, Lívia M L Goulart, Thiago T de S Silva, José L Gava, Joel C Furches, Jason James, Robert B Harrison
In many countries, the main reason for severely restricting or outright banning the land application of class B biosolids is the lack of risk assessment for adverse human health impacts. Among pathogens that are not often studied are helminth ova, including that of the spp. Almost all of the knowledge about the persistence of spp. ova in soils fertilized with biosolids is based on studies developed in North America, Europe, and Asia. These studies have almost always been conducted under temperate climate conditions, which may cause erroneous interpretations when the conclusions are extrapolated to tropical regions such as those found in Brazil...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28724082/prospective-targeted-recombination-and-genetic-gains-for-quantitative-traits-in-maize
#11
Rex Bernardo
Advances in clustered regularly interspaced short palindromic repeats (CRISPR) technology have allowed targeted recombination in specific DNA sequences in yeast (). My objective was to determine if the selection gains from targeted recombination are large enough to warrant the development of targeted recombination technology in plants. Genomewide marker effects for quantitative traits in two maize ( L.) experiments were used to identify targeted recombination points that would maximize the per-chromosome genetic gains in a given cross...
July 2017: Plant Genome
https://www.readbyqxmd.com/read/28724077/physical-map-of-the-short-arm-of-bread-wheat-chromosome-3d
#12
Kateřina Holušová, Jan Vrána, Jan Šafář, Hana Šimková, Barbora Balcárková, Zeev Frenkel, Benoit Darrier, Etienne Paux, Federica Cattonaro, Helene Berges, Thomas Letellier, Michael Alaux, Jaroslav Doležel, Jan Bartoš
Bread wheat ( L.) is one of the most important crops worldwide. Although a reference genome sequence would represent a valuable resource for wheat improvement through genomics-assisted breeding and gene cloning, its generation has long been hampered by its allohexaploidy, high repeat content, and large size. As a part of a project coordinated by the International Wheat Genome Sequencing Consortium (IWGSC), a physical map of the short arm of wheat chromosome 3D (3DS) was prepared to facilitate reference genome assembly and positional gene cloning...
July 2017: Plant Genome
https://www.readbyqxmd.com/read/28724060/genome-wide-association-mapping-of-crown-rust-resistance-in-oat-elite-germplasm
#13
Kathy Esvelt Klos, Belayneh A Yimer, Ebrahiem M Babiker, Aaron D Beattie, J Michael Bonman, Martin L Carson, James Chong, Stephen A Harrison, Amir M H Ibrahim, Frederic L Kolb, Curt A McCartney, Michael McMullen, Jennifer Mitchell Fetch, Mohsen Mohammadi, J Paul Murphy, Nicholas A Tinker
Oat crown rust, caused by f. sp. , is a major constraint to oat ( L.) production in many parts of the world. In this first comprehensive multienvironment genome-wide association map of oat crown rust, we used 2972 single-nucleotide polymorphisms (SNPs) genotyped on 631 oat lines for association mapping of quantitative trait loci (QTL). Seedling reaction to crown rust in these lines was assessed as infection type (IT) with each of 10 crown rust isolates. Adult plant reaction was assessed in the field in a total of 10 location-years as percentage severity (SV) and as infection reaction (IR) in a 0-to-1 scale...
July 2017: Plant Genome
https://www.readbyqxmd.com/read/28724046/breast-cancer-screening-in-brazil-barriers-related-to-the-health-system
#14
René Aloisio da Costa Vieira, Alessandro Formenton, Silvia Regina Bertolini
Objective: Identify factors related to the health system that lead to a late diagnosis of breast cancer in Brazil. Method: We performed a systematic review in the PubMed and LILACS databases using as keywords "Breast cancer," "system of health" and "Brazil or Brasil." We evaluated the content of the articles using the PRISMA methodology based on PICTOS. The final date was 12/16/2015. We were able to identify 94 publications in PubMed and 43 publications in LILACS...
May 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28724031/methodological-challenges-in-the-use-of-focus-groups-with-people-with-severe-mental-illness
#15
Rosana Teresa Onocko-Campos, Alberto Rodolfo Giovanello Díaz, Catarina Magalhães Dahl, Erotildes Maria Leal, Octavio Domont de Serpa
This study addresses the practical, methodological and ethical challenges that were found in three studies that used focus groups with people with severe mental illness, in the context of community mental health services in Brazil. Focus groups are a powerful tool in health research that need to be better discussed in research with people with severe mental illness, in the context of community mental health facilities. This study is based on the authors' experience of conducting and analyzing focus groups in three different cities - Campinas, Rio de Janeiro and Salvador - between 2006-2010...
July 13, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/28724029/-psychosocial-stress-at-work-and-alcohol-consumption-patterns-in-offshore-oil-workers
#16
Jahina Moura Vidal, Angela Mendes Abreu, Luciana Fernandes Portela
The objectives were to assess the association between psychosocial stress at work and alcohol consumption patterns in offshore oil workers. This was a cross-sectional study of 210 workers on offshore oil rigs in the state of Rio de Janeiro, Brazil, from July to September 2014. The data collection instrument was a self-completed multidimensional questionnaire. Exposure to stress was measured by the demand-control model and alcohol consumption pattern was measured with the Alcohol Use Disorders Identification Test (AUDIT)...
July 13, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/28724020/the-home-care-teaching-and-learning-process-in-undergraduate-health-care-degree-courses
#17
Ana Paula Hermann, Maria Ribeiro Lacerda, Mariluci Alves Maftum, Elizabeth Bernardino, Ana Lúcia Schaefer Ferreira de Mello
Home care, one of the services provided by the health system, requires health practitioners who are capable of understanding its specificities. This study aimed to build a substantive theory that describes experiences of home care teaching and learning during undergraduate degree courses in nursing, pharmacy, medicine, nutrition, dentistry and occupational therapy. A qualitative analysis was performed using the grounded theory approach based on the results of 63 semistructured interviews conducted with final year students, professors who taught subjects related to home care, and recent graduates working with home care, all participants in the above courses...
July 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28724010/management-of-primary-care-a-challenge-for-international-cooperation-in-health
#18
Luiz Eduardo Fonseca, Maria Cristina Botelho de Figueiredo, Celina Santos Boga Marques Porto
The need to resolve immediate problems in basic healthcare systems and the decisions that must be made in the daily management of healthcare centers must reach beyond awareness of common sense, and be reinforced by the evidence sought in scientific knowledge that will provide a new look at the facts and phenomena that happen on a daily basis. This article examines an experience of triangular cooperation in health between Angola, Brazil and Japan, which took place in Luanda, Angola between 2011 and 2014. The "Project to Strengthen the Healthcare Through the Development of Human Resources at the Josina Hospital and in other Healthcare Services, and to Revitalize Primary Healthcare in Angola (ProForsa)", with the involvement of Fiocruz as the party executing the primary healthcare component...
July 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28724007/-mal-nutrition-and-the-new-epidemiological-trend-in-a-context-of-development-and-inequalities
#19
Nathália Paula de Souza, Pedro Israel Cabral de Lira, Annick Fontbonne, Fernanda Cristina de Lima Pinto, Eduarda Ângela Pessoa Cesse
The current context of intense social, economic and political transformations has contributed to changes in the health profile of the population and generated discussions about the impact of these changes on the reorganization of society. At the present moment, the increase in non-communicable illnesses and diseases raises major concerns about the paradox of malnutrition and obesity. Based on an in-depth reading and interpretation of texts, documents and databases, this article discusses issues related to nutrition, considering the dimensions of shortages and excesses, the evolution of nutrition over time, and the connection with the new epidemiological trend...
July 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28723999/moving-towards-south-south-international-health-debts-and-challenges-in-the-regional-health-agenda
#20
María Belén Herrero
The aim of this paper is twofold. First, it aims to investigate the increased interest in health as an important dimension of the foreign policy and diplomatic concerns together with the emergence of a new framework for regional health integration and regional health diplomacy. Second, it seeks to understand the role and practices of new regional blocs in the field of health and whether they are conducting to the emergence of new strategies for addressing health regional policies in South America. The regional policy process relates to health as a right...
July 2017: Ciência & Saúde Coletiva
keyword
keyword
10552
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"