keyword
https://read.qxmd.com/read/38341593/non-mitotic-proliferation-of-malignant-cancer-cells-revealed-through-live-cell-imaging-of-primary-and-cell-line-cultures
#21
JOURNAL ARTICLE
Iram Shazia Tyagi, Ho Yin Calvin Tsui, Si Chen, Xinyi Li, Wai-Kin Mat, Muhammad A Khan, Lucas Brendan Choy, Ka-Yin Aden Chan, Tat-Ming Danny Chan, Chi-Ping Stephanie Ng, Ho-Keung Ng, Wai Sang Poon, Hong Xue
INTRODUCTION: Anti-mitosis has been a key strategy of anti-cancer therapies, targeting at a fundamental property of cancer cells, their non-controllable proliferation due to overactive mitotic divisions. For improved anti-cancer therapies, it is important to find out whether cancer cells can proliferate independent of mitosis and become resistant to anti-mitotic agents. RESULTS: In this study, live-cell imaging was applied to both primary-cultures of tumor cells, and immortalized cancer cell lines, to detect aberrant proliferations...
February 10, 2024: Cell Division
https://read.qxmd.com/read/38338708/unveiling-the-antiviral-properties-of-panduratin-a-through-sars-cov-2-infection-modeling-in-cardiomyocytes
#22
JOURNAL ARTICLE
Aung Khine Linn, Suwimon Manopwisedjaroen, Phongthon Kanjanasirirat, Suparerk Borwornpinyo, Suradej Hongeng, Phetcharat Phanthong, Arunee Thitithanyanont
Establishing a drug-screening platform is critical for the discovery of potential antiviral agents against SARS-CoV-2. In this study, we developed a platform based on human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) to investigate SARS-CoV-2 infectivity, with the aim of evaluating potential antiviral agents for anti-SARS-CoV-2 activity and cardiotoxicity. Cultured myocytes of iPSC-CMs and immortalized human cardiomyocyte cell line (AC-16) were primarily characterized for the expression of cardiac markers and host receptors of SARS-CoV-2...
January 24, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38324745/phosphodiesterase-type-5-inhibitors-in-men-with-erectile-dysfunction-and-the-risk-of-alzheimer-disease-a-cohort-study
#23
JOURNAL ARTICLE
Matthew Adesuyan, Yogini H Jani, Dana Alsugeir, Robert Howard, Chengsheng Ju, Li Wei, Ruth Brauer
BACKGROUND AND OBJECTIVES: Repurposing phosphodiesterase type 5 inhibitors (PDE5Is) as drugs for Alzheimer disease (AD) risk reduction has shown promise based on animal studies. However, evidence in humans remains inconclusive. Therefore, we conducted a cohort study to evaluate the association between PDE5I initiation compared with nonuse and the risk of developing AD in men with erectile dysfunction (ED). METHODS: Using electronic health records from IQVIA Medical Research Data UK (formerly known as the THIN database), we identified men aged ≥40 years with a new diagnosis of ED between 2000 and 2017...
February 27, 2024: Neurology
https://read.qxmd.com/read/38319718/construction-of-a-necroptosis-related-lncrna-signature-for-predicting-prognosis-and-revealing-the-immune-microenvironment-in-bladder-cancer
#24
JOURNAL ARTICLE
Jingsong Wang, Zhengyu Jiang, Kai Wang, Qingyuan Zheng, Jun Jian, Xiuheng Liu, Zhiyuan Chen, Rui Yang, Lei Wang
BACKGROUND: Bladder cancer (BCa) is a common malignancy in the urinary system. Necroptosis, a recently discovered form of programmed cell death, is closely associated with the development and progression of various types of tumors. Targeting necroptosis through anti-cancer strategies has shown potential as a therapy for cancer. We aimed to develop a necroptosis-related lncRNAs (NRlncRNAs) risk model that can predict the survival and tumor immunity of BCa patients. METHODS: We analyzed sequencing data obtained from the TCGA database, and applied least absolute shrinkage and selection operator (LASSO) and Cox regression analysis to identify crucial NRlncRNAs for building a risk model...
February 5, 2024: Aging
https://read.qxmd.com/read/38307386/nr2f6-is-essential-for-brown-adipocyte-differentiation-and-systemic-metabolic-homeostasis
#25
JOURNAL ARTICLE
Wei-Yu Zhou, Pei Liu, Yi-Fan Xia, Yi-Jie Shi, Hong-Yu Xu, Meng Ding, Qi-Qi Yang, Shu-Wen Qian, Yan Tang, Yan Lu, Qi-Qun Tang, Yang Liu
OBJECTIVE: Brown adipose tissue (BAT) development and function are essential for maintaining energy balance. However, the key factors that specifically regulate brown adipogenesis require further identification. Here, we demonstrated that the nuclear receptor subfamily 2 group F member 6 (NR2F6) played a pivotal role in brown adipogenesis and energy homeostasis. METHODS: We examined the differentiation of immortalized brown adipocytes and primary brown adipocytes when NR2F6 were deleted, and explored the mechanism through which NR2F6 regulated adipogenesis using ChIP-qPCR in vitro...
January 31, 2024: Molecular Metabolism
https://read.qxmd.com/read/38276496/-lactobacillus-johnsonii-ljo02-dsm-33828-cell-free-supernatant-and-vitamin-d-improve-wound-healing-and-reduce-interleukin-6-production-in-staphylococcus-aureus-infected-human-keratinocytes
#26
JOURNAL ARTICLE
Paola Zanetta, Chiara Ballacchino, Diletta Francesca Squarzanti, Angela Amoruso, Marco Pane, Barbara Azzimonti
Methicillin-resistant biofilm-forming Staphylococcus spp. are found in about 25% of the overall cases of chronic wounds, which can undergo malignant degeneration and be associated with skin cancer. Although antimicrobial agents are clinically used to counteract pathogens and promote wound healing, they are increasingly ineffective against multi-drug resistant bacteria. Moreover, they can induce dysbiosis, which favors opportunistic pathogen infections and alters immune responses. Consequently, research on pathogen containment strategies is crucial...
December 21, 2023: Pharmaceutics
https://read.qxmd.com/read/38260088/mass-production-of-ipsc-derived-platelets-toward-the-clinical-application
#27
REVIEW
Akihiro Kayama, Koji Eto
The ex vivo production of platelets from induced pluripotent cells (iPSCs) may offer a safer and sustainable alternative for transfusions and drug delivery systems (DDS). However, the mass production of the clinically required number of iPSC-derived platelets (iPSC-PLTs) is challenging. Here, we introduce recent technologies for mass production and the first-in-human clinical trial using ex vivo iPSC-PLTs. To this end, we established immortalized megakaryocyte progenitor cell lines (imMKCLs) as an expandable master cell bank (MCB) through the overexpression of c-MYC, BMI1 and BCL-XL, which modulated megakaryopoiesis and thrombopoiesis...
March 2024: Regenerative Therapy
https://read.qxmd.com/read/38258059/an-improved-in-vitro-blood-brain-barrier-model-for-the-evaluation-of-drug-permeability-using-transwell-with-shear-stress
#28
JOURNAL ARTICLE
Junhyeong Kim, Seong-Ah Shin, Chang Sup Lee, Hye Jin Chung
The development of drugs targeting the central nervous system (CNS) is challenging because of the presence of the Blood-Brain barrier (BBB). Developing physiologically relevant in vitro BBB models for evaluating drug permeability and predicting the activity of drug candidates is crucial. The transwell model is one of the most widely used in vitro BBB models. However, this model has limitations in mimicking in vivo conditions, particularly in the absence of shear stress. This study aimed to overcome the limitations of the transwell model using immortalized human endothelial cells (hCMEC/D3) by developing a novel dish design for an orbital shaker, providing shear stress...
December 28, 2023: Pharmaceutics
https://read.qxmd.com/read/38258038/fabrication-and-characterization-of-an-enzyme-triggered-therapeutic-releasing-hydrogel-bandage-contact-lens-material
#29
JOURNAL ARTICLE
Susmita Bose, Chau-Minh Phan, Muhammad Rizwan, John Waylon Tse, Evelyn Yim, Lyndon Jones
PURPOSE: The purpose of this study was to develop an enzyme-triggered, therapeutic-releasing bandage contact lens material using a unique gelatin methacrylate formulation (GelMA+). METHODS: Two GelMA+ formulations, 20% w / v , and 30% w / v concentrations, were prepared through UV polymerization. The physical properties of the material, including porosity, tensile strain, and swelling ratio, were characterized. The enzymatic degradation of the material was assessed in the presence of matrix metalloproteinase-9 (MMP-9) at concentrations ranging from 0 to 300 µg/mL...
December 24, 2023: Pharmaceutics
https://read.qxmd.com/read/38213326/generation-of-mature-toxoplasma-gondii-bradyzoites-in-human-immortalized-myogenic-kd3-cells
#30
JOURNAL ARTICLE
Deborah Maus, Blake Curtis, David Warschkau, Estefanía Delgado Betancourt, Frank Seeber, Martin Blume
Toxoplasma gondii is a zoonotic protozoan parasite and one of the most successful foodborne pathogens. Upon infection and dissemination, the parasites convert into the persisting, chronic form called bradyzoites, which reside within cysts in muscle and brain tissue. Despite their importance, bradyzoites remain difficult to investigate directly, owing to limited in vitro models. In addition, the need for new drugs targeting the chronic stage, which is underlined by the lack of eradicating treatment options, remains difficult to address since in vitro access to drug-tolerant bradyzoites remains limited...
January 5, 2024: Bio-protocol
https://read.qxmd.com/read/38201286/generating-patient-derived-hcc-cell-lines-suitable-for-predictive-in-vitro-and-in-vivo-drug-screening-by-orthotopic-transplantation
#31
JOURNAL ARTICLE
Lisa Staffeldt, Gregor Mattert, Kristoffer Riecken, Götz Rövenstrunk, Anika Volkmar, Asmus Heumann, Mohamed Moustafa, Manfred Jücker, Boris Fehse, Udo Schumacher, Stefan Lüth, Janine Kah
Hepatocellular carcinoma (HCC) results in high mortality due to ineffective systemic therapy. Human immortalized cell lines are commonly used to study anti-tumor effects in the context of new anti-tumor therapies and tumor biology. As immortalized cell lines have limited biological relevance and heterogeneity compared to primary cells, patient-derived tumor tissues, and corresponding immune cells are the gold standards for studying the complexity of individual tumor entities. However, culturing primary HCC cells has a low success rate...
December 30, 2023: Cells
https://read.qxmd.com/read/38191171/cpsf3-inhibition-blocks-pancreatic-cancer-cell-proliferation-through-disruption-of-core-histone-mrna-processing
#32
JOURNAL ARTICLE
Abdulrahman A Alahmari, Aditi H Chaubey, Venkata S Jonnakuti, Arwen A Tisdale, Carla D Schwarz, Abigail C Cornwell, Kathryn E Maraszek, Emily J Paterson, Minsuh Kim, Swati Venkat, Eduardo Cortes Gomez, Jianmin Wang, Katerina V Gurova, Hari Krishna Yalamanchili, Michael E Feigin
Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with limited effective treatment options, potentiating the importance of uncovering novel drug targets. Here, we target Cleavage and Polyadenylation Specificity Factor 3 (CPSF3), the 3' endonuclease that catalyzes mRNA cleavage during polyadenylation and histone mRNA processing. We find that CPSF3 is highly expressed in PDAC and is associated with poor prognosis. CPSF3 knockdown blocks PDAC cell proliferation and colony formation in vitro and tumor growth in vivo...
January 8, 2024: RNA
https://read.qxmd.com/read/38186297/comparative-effectiveness-of-sodium-glucose-cotransporter-2-inhibitors-versus-glucagon-like-peptide-1-receptor-agonists-in-patients-with-type-2-diabetes-and-mild-moderate-chronic-kidney-disease
#33
JOURNAL ARTICLE
Jinnie J Rhee, Jialin Han, Maria E Montez-Rath, Glenn M Chertow
AIM: To determine the comparative effectiveness regarding major cardiovascular events of glucagon-like peptide-1 (GLP-1) receptor agonists and sodium-glucose cotransporter-2 (SGLT-2) inhibitors in patients with type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). MATERIALS AND METHODS: We assembled a cohort of commercially insured adult patients with T2DM in the United States (derived from Optum Clinformatics DataMart 2003-2021) who were new users of GLP-1 receptor agonists or SGLT-2 inhibitors...
January 8, 2024: Diabetes, Obesity & Metabolism
https://read.qxmd.com/read/38149884/recent-advances-in-the-development-of-monoclonal-antibodies-and-next-generation-antibodies
#34
JOURNAL ARTICLE
Rohit Singh, Pankaj Chandley, Soma Rohatgi
mAbs are highly indispensable tools for diagnostic, prophylactic, and therapeutic applications. The first technique, hybridoma technology, was based on fusion of B lymphocytes with myeloma cells, which resulted in generation of single mAbs against a specific Ag. Along with hybridoma technology, several novel and alternative methods have been developed to improve mAb generation, ranging from electrofusion to the discovery of completely novel technologies such as B cell immortalization; phage, yeast, bacterial, ribosome, and mammalian display systems; DNA/RNA encoded Abs; single B cell technology; transgenic animals; and artificial intelligence/machine learning...
December 1, 2023: ImmunoHorizons
https://read.qxmd.com/read/38138080/the-research-progress-on-immortalization-of-human-b-cells
#35
REVIEW
Huiting Xu, Xinxin Xiang, Weizhe Ding, Wei Dong, Yihong Hu
Human B cell immortalization that maintains the constant growth characteristics and antibody expression of B cells in vitro is very critical for the development of antibody drugs and products for the diagnosis and bio-therapeutics of human diseases. Human B cell immortalization methods include Epstein-Barr virus (EBV) transformation, Simian virus 40 (SV40) virus infection, in vitro genetic modification, and activating CD40, etc. Immortalized human B cells produce monoclonal antibodies (mAbs) very efficiently, and the antibodies produced in this way can overcome the immune rejection caused by heterologous antibodies...
December 7, 2023: Microorganisms
https://read.qxmd.com/read/38136356/drug-responses-in-plexiform-neurofibroma-type-i-pnf1-cell-lines-using-high-throughput-data-and-combined-effectiveness-and-potency
#36
JOURNAL ARTICLE
Paul O Zamora, Gabriel Altay, Ulisses Santamaria, Nathan Dwarshuis, Hari Donthi, Chang In Moon, Dana Bakalar, Matthew Zamora
Background : Neurofibromatosis type 1 (NF1) is a genetic disorder characterized by heterozygous germline NF1 gene mutations that predispose patients to developing plexiform neurofibromas, which are benign but often disfiguring tumors of the peripheral nerve sheath induced by loss of heterozygosity at the NF1 locus. These can progress to malignant peripheral nerve sheath tumors (MPNSTs). There are no approved drug treatments for adults with NF1-related inoperable plexiform neurofibromas, and only one drug (selumetinib), which is an FDA-approved targeted therapy for the treatment of symptomatic pediatric plexiform neurofibromas, highlighting the need for additional drug screening and development...
December 12, 2023: Cancers
https://read.qxmd.com/read/38134573/establishment-of-an-immortalized-cell-line-derived-from-human-adenomyosis-ectopic-lesions
#37
JOURNAL ARTICLE
Zilu Wang, Fengxin Cui, Yinuo Chen, Hongyun Liu, Yiran Zhang, Yaxin Shi, Yinuo Zhang, Yanfei Wang, Na Liang, Li Xu, Ying Liu, Zhiyong Liu, Wei Shi
Because adenomyosis (AM) ectopic primary cells are hard to come by, have a short lifespan, and the characteristics that alter over time, their utility in AM research is constrained. This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells...
December 1, 2023: Tissue & Cell
https://read.qxmd.com/read/38128884/erythrocyte-membrane-coated-nanocarriers-modified-by-tgn-for-alzheimer-s-disease
#38
JOURNAL ARTICLE
Jinlian Gu, Chang Yan, Shun Yin, Hao Wu, Chi Liu, Ao Xue, Xia Lei, Ning Zhang, Fang Geng
Alzheimer's disease (AD) is an aging-related neurodegenerative disease, and the main pathological feature was β-amyloid protein (Aβ) deposition. Recently, bioactive materials-based drug delivery system has been widely investigated for the treatment of AD. In this study, we developed a red blood cells (RBC) membrane-coated polycaprolactone (PCL) nanoparticles (NPs) loading with a therapeutic agent for AD, curcumin (Cur). A functional peptide TGNYKALHPHN (TGN) was conjugated to the surface of membrane for blood-brain barrier (BBB) transport (TGN-RBC-NPs-Cur)...
December 19, 2023: Journal of Controlled Release
https://read.qxmd.com/read/38101570/ex-vivo-discovery-of-synergistic-drug-combinations-for-hematologic-malignancies
#39
JOURNAL ARTICLE
Kamran A Ali, Reecha D Shah, Anukriti Dhar, Nina M Myers, Cameron Nguyen, Arisa Paul, Jordan E Mancuso, A Scott Patterson, James P Brody, Diane Heiser
Combination therapies have improved outcomes for patients with acute myeloid leukemia (AML). However, these patients still have poor overall survival. Although many combination therapies are identified with high-throughput screening (HTS), these approaches are constrained to disease models that can be grown in large volumes (e.g., immortalized cell lines), which have limited translational utility. To identify more effective and personalized treatments, we need better strategies for screening and exploring potential combination therapies...
December 13, 2023: SLAS Discovery
https://read.qxmd.com/read/38100899/immunomodulator-use-risk-factors-and-management-of-flares-and-mortality-for-patients-with-pre-existing-rheumatoid-arthritis-after-immune-checkpoint-inhibitors-for-cancer
#40
JOURNAL ARTICLE
Kaitlin R McCarter, Senada Arabelovic, Xiaosong Wang, Taylor Wolfgang, Kazuki Yoshida, Grace Qian, Emily N Kowalski, Kathleen M M Vanni, Nicole R LeBoeuf, Elizabeth I Buchbinder, Lydia Gedmintas, Lindsey A MacFarlane, Deepak A Rao, Nancy A Shadick, Ellen M Gravallese, Jeffrey A Sparks
OBJECTIVE: To investigate immunomodulator use, risk factors and management for rheumatoid arthritis (RA) flares, and mortality for patients with pre-existing RA initiating immune checkpoint inhibitors (ICI) for cancer. METHODS: We performed a retrospective cohort study of all patients with RA meeting 2010 ACR/EULAR criteria that initiated ICI for cancer at Mass General Brigham or Dana-Farber Cancer Institute in Boston, MA (2011-2022). We described immunomodulator use and changes at baseline of ICI initiation...
December 8, 2023: Seminars in Arthritis and Rheumatism
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