keyword
https://read.qxmd.com/read/38505264/profiling-of-petroselinum-sativum-mill-fuss-phytoconstituents-and-assessment-of-their-biocompatibility-antioxidant-anti-aging-wound-healing-and-antibacterial-activities
#21
JOURNAL ARTICLE
Ismail Mahdi, Paola Imbimbo, Hassan Annaz, Widad Ben Bakrim, Nihad Sahri, Asmae Alaoui, Daria Maria Monti, Mansour Sobeh
Petroselinum sativum , known as parsley, is a fragrant herb that possesses a rich heritage of utilization in traditional medicinal practices. In this study, we annotated the phytocontents of the aqueous and ethanolic extracts of P. sativum and investigated their antioxidant, cytoprotective, antiaging, wound healing, and antibacterial activities. LC-MS/MS analysis of both extracts revealed the presence of 47 compounds belonging to diverse groups including organic acids, phenolic acids, and flavonoids. By MTT assay, the extracts were fully biocompatible on immortalized human keratinocytes (HaCaT) while they inhibited intracellular ROS formation (DCFDA assay) and prevented GSH depletion (DTNB assay) upon UVA exposure...
2024: Frontiers in Nutrition
https://read.qxmd.com/read/38504290/a-partial-drp1-knockout-improves-autophagy-flux-independent-of-mitochondrial-function
#22
JOURNAL ARTICLE
Rebecca Z Fan, Carolina Sportelli, Yanhao Lai, Said S Salehe, Jennifer R Pinnell, Harry J Brown, Jason R Richardson, Shouqing Luo, Kim Tieu
BACKGROUND: Dynamin-related protein 1 (Drp1) plays a critical role in mitochondrial dynamics. Partial inhibition of this protein is protective in experimental models of neurological disorders such as Parkinson's disease and Alzheimer's disease. The protective mechanism has been attributed primarily to improved mitochondrial function. However, the observations that Drp1 inhibition reduces protein aggregation in such neurological disorders suggest the involvement of autophagy. To investigate this potential novel protective mechanism of Drp1 inhibition, a model with impaired autophagy without mitochondrial involvement is needed...
March 19, 2024: Molecular Neurodegeneration
https://read.qxmd.com/read/38503846/endogenous-bax-and-bak-form-mosaic-rings-of-variable-size-and-composition-on-apoptotic-mitochondria
#23
JOURNAL ARTICLE
Sarah V Schweighofer, Daniel C Jans, Jan Keller-Findeisen, Anne Folmeg, Peter Ilgen, Mark Bates, Stefan Jakobs
One hallmark of apoptosis is the oligomerization of BAX and BAK to form a pore in the mitochondrial outer membrane, which mediates the release of pro-apoptotic intermembrane space proteins into the cytosol. Cells overexpressing BAX or BAK fusion proteins are a powerful model system to study the dynamics and localization of these proteins in cells. However, it is unclear whether overexpressed BAX and BAK form the same ultrastructural assemblies following the same spatiotemporal hierarchy as endogenously expressed proteins...
March 19, 2024: Cell Death and Differentiation
https://read.qxmd.com/read/38502482/hchcr-high-throughput-single-cell-imaging-of-rna-in-human-primary-immune-cells
#24
JOURNAL ARTICLE
Manasi Gadkari, Jing Sun, Adrian Carcamo, Iain Fraser, Luis M Franco, Gianluca Pegoraro
Functional genomics and chemical screens can identify and characterize novel cellular factors regulating signaling networks and chemical tools to modulate their function for the treatment of disease. Screening methods have relied primarily on immortalized and/or transformed cancer cell lines, which can limit the generalization of results to more physiologically relevant systems. Most have also relied on immunofluorescence, or on stably expressed recombinant fluorescent proteins, to detect specific protein markers using high-content imaging readouts...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38502449/placental-trophoblast-cell-isolation-from-the-term-placenta
#25
JOURNAL ARTICLE
Manuel Vidal Jr S, Radnaa Enkhtuya, Rheanna Urrabaz-Garza, Ramkumar Menon
Multiple cell lines have been utilized over time in studying placental biology. Still, most of them rely on choriocarcinoma cells or immortalized trophoblast cells that may not be entirely comparable with actual human placental trophoblast cells. Term placentas can be a source of primary villous trophoblasts. However, challenges remain in isolating them and maintaining them in extended culture. This manuscript describes our three-phase protocol utilizing enzymatic/mechanical digestion, modified Percoll gradient density separation, and immunopurification using magnetic beads...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38502447/feto-maternal-interface-organ-on-chip-a-new-technology-to-study-ascending-infection
#26
JOURNAL ARTICLE
Giovana Fernanda Cosi Bento, Márcia Guimarães da Silva, Ramkumar Menon, Lauren S Richardson
Modeling human pregnancy is challenging as two subjects, the mother and fetus, must be evaluated in tandem. To understand pregnancy, parturition, and adverse pregnancy outcomes, the two feto-maternal interfaces (FMi) that form during gestation (i.e., the placenta and fetal membrane) need to be investigated to understand their biological roles, and organ dysfunction can lead to adverse outcomes. Adverse pregnancy outcomes such as preterm rupture of the membranes, spontaneous preterm birth, preeclampsia, intra-uterine growth restriction, and gestational diabetes rates are on the rise worldwide, highlighting the need for future studies and a better understanding of molecular and cellular pathways that contribute to disease onset...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38502139/expression-of-atp-binding-cassette-transporter-a1-abca1-in-eyelid-tissues-and-meibomian-gland-epithelial-cells
#27
JOURNAL ARTICLE
Fang Zheng, Jingjing Su, Jiaoman Wang, Qing Zhan, Mei Su, Sicheng Ding, Wei Li, Ying-Ting Zhu, Ping Guo
PURPOSE: To validate the adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1) expression and distribution in human eyelid tissues and meibomian gland epithelial cells. METHODS: Meibomian gland tissues from human eyelids were isolated by collagenase A digestion and cultured in defined keratinocyte serum-free medium (DKSFM). Infrared imaging was used to analyze the general morphology of meibomian glands. Hematoxylin and eosin (H&E) staining and Oil Red O staining were used to observe the morphological structure and lipid secretion in the human meibomian gland tissues...
March 5, 2024: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/38501905/association-of-freeze-dried-plasma-with-24-h-mortality-among-trauma-patients-at-risk-for-hemorrhage
#28
JOURNAL ARTICLE
Nee-Kofi Mould-Millman, Adane F Wogu, Bailey K Fosdick, Julia M Dixon, Brenda L Beaty, Smitha Bhaumik, Hendrick J Lategan, Willem Stassen, Steven G Schauer, Elmin Steyn, Janette Verster, Craig Wylie, Shaheem de Vries, Maria Jamison, Maria Kohlbrenner, Mohammed Mayet, Lesley Hodsdon, Leigh Wagner, L' Oreal Snyders, Karlien Doubell, Denise Lourens, Vikhyat S Bebarta
BACKGROUND: Blood products form the cornerstone of contemporary hemorrhage control but are limited resources. Freeze-dried plasma (FDP), which contains coagulation factors, is a promising adjunct in hemostatic resuscitation. We explore the association between FDP alone or in combination with other blood products on 24-h mortality. STUDY DESIGN AND METHODS: This is a secondary data analysis from a cross-sectional prospective observational multicenter study of adult trauma patients in the Western Cape of South Africa...
March 19, 2024: Transfusion
https://read.qxmd.com/read/38501828/differentiation-of-keratinocytes-or-exposure-to-type-2-cytokines-diminishes-s-aureus-internalization
#29
JOURNAL ARTICLE
Alexis R Morgenstern, Liam F Peterson, Kimberly A Arnold, Matthew G Brewer
Staphylococcus aureus is a leading cause of skin and soft tissue infections. Colonization by this bacterium is increased in individuals with chronic cutaneous diseases such as atopic dermatitis, psoriasis, and bullous pemphigoid. The greater abundance of S. aureus on the skin of subjects with atopic dermatitis in particular has been linked to recurrent cutaneous infections. The primary cell type of the epidermal layer of the skin is the keratinocyte, and it is thought that S. aureus internalized in keratinocytes associates with an increased incidence of skin infections...
March 19, 2024: MSphere
https://read.qxmd.com/read/38499143/a-comparison-between-different-human-hepatocyte-models-reveals-profound-differences-in-net-glucose-production-lipid-composition-and-metabolism-in-vitro
#30
JOURNAL ARTICLE
Flavio Bonanini, Madhulika Singh, Hong Yang, Dorota Kurek, Amy C Harms, Adil Mardinoglu, Thomas Hankemeier
Hepatocytes are responsible for maintaining a stable blood glucose concentration during periods of nutrient scarcity. The breakdown of glycogen and de novo synthesis of glucose are crucial metabolic pathways deeply interlinked with lipid metabolism. Alterations in these pathways are often associated with metabolic diseases with serious clinical implications. Studying energy metabolism in human cells is challenging. Primary hepatocytes are still considered the golden standard for in vitro studies and have been instrumental in elucidating key aspects of energy metabolism found in vivo...
March 16, 2024: Experimental Cell Research
https://read.qxmd.com/read/38498295/from-pluripotent-stem-cells-to-organoids-and-bioprinting-recent-advances-in-dental-epithelium-and-ameloblast-models-to-study-tooth-biology-and-regeneration
#31
REVIEW
Florian Hermans, Steffie Hasevoets, Hugo Vankelecom, Annelies Bronckaers, Ivo Lambrichts
Ameloblasts are the specialized dental epithelial cell type responsible for enamel formation. Following completion of enamel development in humans, ameloblasts are lost and biological repair or regeneration of enamel is not possible. In the past, in vitro models to study dental epithelium and ameloblast biology were limited to freshly isolated primary cells or immortalized cell lines, both with limited translational potential. In recent years, large strides have been made with the development of induced pluripotent stem cell and organoid models of this essential dental lineage - both enabling modeling of human dental epithelium...
March 18, 2024: Stem cell reviews and reports
https://read.qxmd.com/read/38496506/htlv-1-infected-t-cells-cause-bone-loss-via-small-extracellular-vesicles
#32
Nitin Kumar Pokhrel, Amanda Panfil, Haniya Habib, Sham Seeniraj, Ancy Joseph, Daniel Rauch, Linda Cox, Robert Sprung, Petra Erdmann Gilmore, Qiang Zhang, R Reid Townsend, Lianbo Yu, Ayse Selen Yilmaz, Rajeev Aurora, William Park, Lee Ratner, Katherine N Weilbaecher, Deborah J Veis
Adult T cell leukemia (ATL), caused by infection with human T cell leukemia virus type 1 (HTLV-1), is often complicated by hypercalcemia and osteolytic lesions. Therefore, we studied the communication between patient-derived ATL cells (ATL-PDX) and HTLV-1 immortalized CD4+ T cell lines (HTLV/T) with osteoclasts and their effects on bone mass in mice. Intratibial inoculation of some HTLV/T lead to a profound local decrease in bone mass similar to marrow-replacing ATL-PDX, despite the fact that few HTLV/T cells persisted in the bone...
March 4, 2024: bioRxiv
https://read.qxmd.com/read/38494987/bsa-adsorption-on-titanium-dioxide-nanoparticle-surfaces-for-controlling-their-cellular-uptake-in-skin-cells
#33
JOURNAL ARTICLE
Raweewan Thiramanas, Yodsathorn Wongngam, Goragot Supanakorn, Duangporn Polpanich
Nanoparticles (NPs) are continuously being developed for many applications including imaging, biomedicine, and everyday products. It is difficult to avoid contact with NPs such as titanium dioxide (TiO2 ) NPs, which are widely used in sunscreens. However, the safety of TiO2 NPs for skin contact and inhalation remains controversial. If NPs cannot penetrate the skin, they will be unable to circulate in the bloodstream, accumulate in the body, or cause side effects, ensuring their safety. Therefore, this study aimed to modify TiO2 NP surfaces to inhibit their uptake in skin cells...
March 18, 2024: ACS Applied Bio Materials
https://read.qxmd.com/read/38494833/effect-of-off-label-targeted-drugs-on-long-term-survival-in-chronic-thromboembolic-pulmonary-hypertension-insights-from-a-national-multicentre-prospective-registry
#34
JOURNAL ARTICLE
Wanying Xia, Yuling Qian, Yangyi Lin, Ruilin Quan, Yuanhua Yang, Zhenwen Yang, Hongyan Tian, Shengqing Li, Jieyan Shen, Yingqun Ji, Qing Gu, Huijun Han, Changming Xiong, Jianguo He
BACKGROUND AND OBJECTIVE: Off-label pulmonary arterial hypertension (PAH)-targeted drugs are commonly prescribed for non-operated chronic thromboembolic pulmonary hypertension (CTEPH), but their effect on the long-term prognosis of CTEPH remains unknown. This study investigated the effect of off-label PAH-targeted drugs on the long-term survival of CTEPH patients. METHODS: CTEPH patients were enrolled from a prospective multicentre national registry. Except for licensed riociguat and treprostinil, other PAH-targeted drugs were off-label...
March 17, 2024: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://read.qxmd.com/read/38494079/dna-reference-reagents-for-genotyping-rh-variants
#35
JOURNAL ARTICLE
Emilia Sippert, Evgeniya Volkova, Meagan Rippee-Brooks, Gregory A Denomme, Willy A Flegel, Christine Lee, Richardae Araojo, Orieji Illoh, Zhugong Liu, Maria Rios
Patients who carry RH blood group variants may develop Rh alloantibodies requiring matched red cell transfusions. Serologic reagents for Rh variants often fail to specifically identify variant Rh antigens and are in limited supply. Therefore, red cell genotyping assays are essential for managing transfusions in patients with clinically relevant Rh variants. Well-characterized DNA reference reagents are needed to ensure quality and accuracy of the molecular tests. Eight lyophilized DNA reference reagents, representing 21 polymorphisms in RHD and RHCE, were produced from an existing repository of immortalized B-lymphoblastoid cell lines at CBER/U...
March 15, 2024: Journal of Molecular Diagnostics: JMD
https://read.qxmd.com/read/38490101/enhancer-infestation-drives-tumorigenic-activation-of-inactive-b-compartment-in-epstein-barr-virus-positive-nasopharyngeal-carcinoma
#36
JOURNAL ARTICLE
Harue Mizokami, Atsushi Okabe, Ruchi Choudhary, Masato Mima, Kenta Saeda, Masaki Fukuyo, Bahityar Rahmutulla, Motoaki Seki, Boon-Cher Goh, Satoru Kondo, Hirotomo Dochi, Makiko Moriyama-Kita, Kiyoshi Misawa, Toyoyuki Hanazawa, Patrick Tan, Tomokazu Yoshizaki, Melissa Jane Fullwood, Atsushi Kaneda
BACKGROUND: Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignant epithelial tumor endemic to Southern China and Southeast Asia. While previous studies have revealed a low frequency of gene mutations in NPC, its epigenomic aberrations are not fully elucidated apart from DNA hypermethylation. Epigenomic rewiring and enhancer dysregulation, such as enhancer hijacking due to genomic structural changes or extrachromosomal DNA, drive cancer progression. METHODS: We conducted Hi-C, 4C-seq, ChIP-seq, and RNA-seq analyses to comprehensively elucidate the epigenome and interactome of NPC using C666-1 EBV(+)-NPC cell lines, NP69T immortalized nasopharyngeal epithelial cells, clinical NPC biopsy samples, and in vitro EBV infection in HK1 and NPC-TW01 EBV(-) cell lines...
March 14, 2024: EBioMedicine
https://read.qxmd.com/read/38490094/n-linked-%C3%AE-2-6-sialylation-of-integrin-%C3%AE-1-by-the-sialyltransferase-st6gal1-promotes-cell-proliferation-and-stemness-in-gestational-trophoblastic-disease
#37
JOURNAL ARTICLE
Jianwei Liu, Xinyue Dong, Ru Xie, Ying Tang, Aline M Thomas, Shen Li, Shuai Liu, Ming Yu, Huamin Qin
INTRODUCTION: Gestational trophoblastic disease (GTD) encompasses a spectrum of rare pre-malignant and malignant entities originating from trophoblastic tissue, including partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma. β-galactoside α2,6 sialyltransferase 1 (ST6Gal1), the primary sialyltransferase responsible for the addition of α2,6 sialic acids, is strongly associated with the occurrence and development of several tumor types. However, the role of ST6Gal1/α2,6 -sialylation of trophoblast cells in GTD is still not well understood...
March 11, 2024: Placenta
https://read.qxmd.com/read/38488377/hand-of-the-42-armed-avalokite%C3%A5-vara-holding-a-skull-rod
#38
JOURNAL ARTICLE
Sung Gyun Jung, Kun Hwang, Young Joong Hwang
The skull most commonly symbolizes death, mortality, and the unachievable nature of immortality. However, in Korean Buddhist art, skulls are rarely found. The aim of this study was to explore the image and meaning of the skull in the Joseon dynasty (1392 to 1910) of Korea. The terms [Buddhist] and [painting] and [Korea] were used to search Google (www.google.com), and the Research Information Sharing Service of Korea (https://www-riss-kr-ssl.openlink), was also searched for [Buddhism] () and [art] () and [skull] ()...
March 15, 2024: Journal of Craniofacial Surgery
https://read.qxmd.com/read/38484644/il-13-facilitates-ferroptotic-death-in-asthmatic-epithelial-cells-via-socs1-mediated-ubiquitinated-degradation-of-slc7a11
#39
JOURNAL ARTICLE
Manli Miao, Min Pan, Xu Chen, Jiapan Shen, Ling Zhang, Xiaoxia Feng, Mengting Chen, Guofeng Cui, Huaiyuan Zong, Wen Zhang, Shuang Chang, Fangzhou Xu, Zixi Wang, Dapeng Li, Weiwei Liu, Zhao Ding, Shengquan Zhang, Biao Chen, Xiaojun Zha, Xiaoyun Fan
Th2-high asthma is characterized by elevated levels of type 2 cytokines, such as interleukin 13 (IL-13), and its prevalence has been increasing worldwide. Ferroptosis, a recently discovered type of programmed cell death, is involved in the pathological process of Th2-high asthma; however, the underlying mechanisms remain incompletely understood. In this study, we demonstrated that the serum level of malondialdehyde (MDA), an index of lipid peroxidation, positively correlated with IL-13 level and negatively correlated with the predicted forced expiratory volume in 1 s (FEV1%) in asthmatics...
March 8, 2024: Redox Biology
https://read.qxmd.com/read/38482555/tnik-depletion-induces-inflammation-and-apoptosis-ininjured-renal-proximal-tubule-epithelial-cells
#40
JOURNAL ARTICLE
Shayna T J Bradford, Haojia Wu, Yuhei Kirita, Changfeng Chen, Nicole P Malvin, Yasuhiro Yoshimura, Yoshiharu Muto, Benjamin D Humphreys
In the aftermath of acute kidney injury (AKI), surviving proximal tubule epithelia repopulate injured tubules to promote repair. However, a portion of cells fail to repair (termed Failed Repair Proximal Tubule Cells, FR-PTCs) and exert ongoing proinflammatory and profibrotic effects. To better understand the molecular drivers of the FR-PTC state, we reanalyzed a mouse ischemia-reperfusion injury single nucleus RNA-sequencing (snRNA-seq) atlas to identify Traf2 and Nck Interacting Kinase, Tnik, to be exclusively expressed in FR-PTCs, but not in healthy or acutely injured proximal tubules post AKI (2 and 6 weeks) in mice...
March 14, 2024: American Journal of Physiology. Renal Physiology
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