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Xiaoquan Ding, Xingwang Liu, Jun Chen, Shiyi Chen
Objective : To review the basical research progress of porous tantalum in bone tissue engineering. Methods: The related basical research in fabrication, cytobiology, and surface modification of porous tantalum was reviewed and analyzed. Results: The outstanding physiochemical properties of porous tantalum granted its excellent performance in biocompatibility and osteointegration, as well as promoting cartilage and tendon tissue restoration...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Rasmi R Mishra, Nevin Belder, Suhail A Ansari, Merve Kayhan, Hilal Bal, Umar Raza, Pelin G Ersan, Ünal M Tokat, Erol Eyüpoğlu, Özge Saatci, Pouria Jandaghi, Stefan Wiemann, Ayşegül Üner, Caglar Cekic, Yasser Riazalhosseini, Özgür Şahin
Purpose: Tamoxifen remains an important hormonal therapy for ER-positive breast cancer; however, development of resistance is a major obstacle in clinics. Here, we aimed to identify novel mechanisms of tamoxifen resistance and provide actionable drug targets overcoming resistance. Experimental Design: Whole-transcriptome sequencing, downstream pathway analysis, and drug repositioning approaches were used to identify novel modulators [here: phosphodiesterase 4D (PDE4D)] of tamoxifen resistance. Clinical data involving tamoxifen-treated patients with ER-positive breast cancer were used to assess the impact of PDE4D in tamoxifen resistance...
April 15, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Chuang Zhang, Wenxue Wang, Wenhui He, Ning Xi, Yuechao Wang, Lianqing Liu
Studies on the contractile dynamics of heart cells have attracted broad attention for the development of both heart disease therapies and cardiomyocyte-actuated micro-robotics. In this study, a linear dynamic model of a single cardiomyocyte cell was proposed at the subcellular scale to characterize the contractile behaviors of heart cells, with system parameters representing the mechanical properties of the subcellular components of living cardiomyocytes. The system parameters of the dynamic model were identified with the cellular beating pattern measured by a scanning ion conductance microscope...
January 9, 2018: Biophysical Journal
Dong Lin, Xiaozhen Huang, Wanyu Zhuang, Lili Lin
Through the systematic analysis on the primo vascular system (PVS) in recent years, we believe that in recent years, more and more studies have indicated that PVS is distributed in reticulate structure in every part of body, such as vessels, lymphangions, nerves, brain, spinal cords and internal organs, and it contains a large amount of immunocytes and has involved in the physiological or pathological process of the immunity and circulation in the body. There are the evidences to prove that in morphology and cytobiology...
January 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Weiwei Gao, Runxia Shao, Xiaoping Zhang, Daijian Liu, Ying Liu, Xian'en Fa
Pulmonary arterial hypertension (PAH), characterized by excessive proliferation and apoptosis resistance of pulmonary artery smooth muscle cells (PASMCs), is closely associated with the imbalance in vasoactive mediators and massive remodeling of pulmonary vasculature. DJ-1/park7, a multifunctional protein, plays a critical defense role in several cytobiological activity, such as transcriptional regulation, anti-oxidative stress and tumor formation. In this study, we investigated the effects of DJ-1 on hypoxia-induced PAH model rats and PASMCs, as well as its possible molecular mechanism...
December 1, 2017: Experimental Cell Research
Shenglei Feng, Fupo He, Jiandong Ye
In this study, hierarchically porous calcium phosphate scaffolds (HTCP) with unidirectional pores, transversely interconnected pores, and micropores were fabricated by the combination of extrusion and porogen burnout technique. Gelatin was incorporated into the HTCP scaffolds by vacuum-impregnation of gelatin solution and subsequent freeze-drying. The phase composition, microstructure, physical and cytobiological properties were analyzed. The results showed that the HTCP scaffolds were composed of β-tricalcium phosphate with minor hydroxyapatite...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Bo Pang, Nan Wu, Rongwei Guan, Lin Pang, Xinlei Li, Su Li, Liudi Tang, Ying Guo, Jialei Chen, Donglin Sun, Haiming Sun, Jialin Dai, Jing Bai, Guohua Ji, Peng Liu, An Liu, Qiushi Wang, Sheng Xiao, Songbin Fu, Yan Jin
Purpose: Investigate the role of regulator of chromosome condensation 2 (RCC2) on lung adenocarcinoma (LUAD) metastasis.Experimental Design: Clinical specimens were used to assess the impact of RCC2 on LUAD metastasis. Mouse models, cytobiology, and molecular biology assays were performed to elucidate the function and underlying mechanisms of RCC2 in LUAD.Results: RCC2 expression was frequently increased in LUADs (88/122, 72.13%). It was confirmed by analysis of a larger cohort of TCGA RNA-seq data containing 488 LUADs and 58 normal lung tissues (P < 0...
June 12, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Jiali Zhu, Shan Sun, Kai Jiang, Yuhui Wang, Wenqing Liu, Hengwei Lin
Herein, a highly sensitive and selective fluorimetric nanoprobe for peroxynitrite (ONOO(-)) detection based on photoinduced electron transfer (PET) from ferrocene (Fc) to carbon dots (CDs) is reported. The nanoprobe (named CDs-Fc) can be facilely constructed through covalently conjugating CDs and ferrocenecarboxylic acid. Further studies reveal that the energy level of highest occupied molecular orbital (HOMO) of the CDs is lowered with the addition of ONOO(-) due to its oxidation and nitration capabilities...
May 31, 2017: Biosensors & Bioelectronics
Chuang Zhang, Jialin Shi, Wenxue Wang, Ning Xi, Yuechao Wang, Lianqing Liu
OBJECTIVE: The mechanical properties of cells, which are the main characteristics determining their physical performance and physiological functions, have been actively studied in the fields of cytobiology and biomedical engineering and for the development of medicines. In this study, an indentation-vibration-based method is proposed to simultaneously measure the mechanical properties of cells in situ, including cellular mass (m), elasticity (k), and viscosity (c). METHODS: The proposed measurement method is implemented based on the principle of forced vibration stimulated by simple harmonic force using an atomic force microscope (AFM) system integrated with a piezoelectric transducer as the substrate vibrator...
December 2017: IEEE Transactions on Bio-medical Engineering
Jinyi Feng, Ruilong Lan, Guanxiong Cai, Jinluan Lin, Xinwen Wang, Jianhua Lin, Deping Han
BACKGROUND: The study aimed to explore the correlation between the expression of TREX1 and the metastasis and the survival time of patients with osteosarcoma as well as biological characteristics of osteosarcoma cells for the prognosis judgment of osteosarcoma. METHOD: The correlation between the expression of TREX1 protein and the occurrence of pulmonary metastasis in 45 cases of osteosarcoma was analyzed. The CD133(+) and CD133(-) cell subsets of osteosarcoma stem cells were sorted by the flow cytometry...
November 24, 2016: Journal of Orthopaedic Surgery and Research
Xiaoxue Wu, Shan Sun, Yuhui Wang, Jiali Zhu, Kai Jiang, Yumin Leng, Qinghai Shu, Hengwei Lin
Mitochondria, the power generators in cell, are a primary organelle of oxygen consumption and a main source of reactive oxygen/nitrogen species (ROS/RNS). Peroxynitrite (ONOO(-)), known as a kind of RNS, has been considered to be a significant factor in many cell-related biological processes, and there is great desire to develop fluorescent probes that can sensitively and selectively detect peroxynitrite in living cells. Herein, we developed a fluorescent carbon-dots (C-dots) based mitochondria-targetable nanoprobe with high sensitivity and selectivity for peroxynitrite sensing in living cells...
April 15, 2017: Biosensors & Bioelectronics
Ying Chen, Shuyan Cao, Pingping Xu, Wei Han, Tiankai Shan, Jingying Pan, Weiwei Lin, Xue Chen, Xiaodong Wang
BACKGROUND Results from DNA microarray experiments have shown that the expression of miR-34s undergoes significant changes following spinal cord injury (SCI). The present study was designed to detect changes in the expression of miR-34s and its target genes during the acute and sub-acute stages of SCI. MATERIAL AND METHODS Luxol fast blue (LFB) staining for myelin was used to observe the differences in the general morphology of the spinal cord after SCI in a contusion model in rats. qPCR was carried out to determine the expression variation of miR-34s and its target genes during the acute and sub-acute stages of SCI...
October 25, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
J Nowotny, D Aibibu, J Farack, U Nimtschke, M Hild, M Gelinsky, P Kasten, Ch Cherif
One possibility to improve the mechanical properties after tendon ruptures is augmentation with a scaffold. Based on wet spinning technology, chitosan fibres were processed to a novel pure high-grade multifilament yarn with reproducible quality. The fibres were braided to obtain a 3D tendon scaffold. The CS fibres and scaffolds were evaluated biomechanically and compared to human supraspinatus (SSP) tendons. For the cytobiological characterization, in vitro cell culture experiments with human mesenchymal stem cells (hMSC) were performed...
July 2016: Journal of Biomaterials Science. Polymer Edition
Zi-Jing Lin, Jia Ming, Lu Yang, Jun-Ze Du, Ning Wang, Hao-Jun Luo
BACKGROUND: MicroRNA-206 (miR-206) and connexin 43 (Cx43) are related with the distant metastasis of breast cancer. It remains unclear whether the regulatory effect of miR-206 on Cx43 is involved in metastasis of breast cancer. METHODS: Using quantitative real-time polymerase chain reaction and Western blot, the expressions of miR-206 and Cx43 were determined in breast cancer tissues, hepatic and pulmonary metastasis (PM), and cell lines (MCF-10A, MCF-7, and MDA-MB-231)...
February 20, 2016: Chinese Medical Journal
Shan He, Ling-qiang Zhang
Protein ubiquitination plays vital roles in regulating various cytobiological processes such as cell cycle progression, DNA damage repair, signal transduction and membrane localization of various proteins. Moreover, proteins can be modified by single ubiquitin molecules (monoubiquitination) or ubiquitin chains (polyubiquitination). Polyubiquitination regulates protein function by linking different types of polyubiquitin chains to substrates. All the 7 known linkage types of polyubiquitination are inter-ubiquitin linkages formed through lysine residues...
September 2015: Yi Chuan, Hereditas
Peng Xiu, Xin Zhou, Cuifang Kuang, Yingke Xu, Xu Liu
The refractive index (RI) distribution can serve as a natural label for undyed cell imaging. However, the majority of images obtained through quantitative phase microscopy is integrated along the illumination angle and cannot reflect additional information about the refractive map on a certain plane. Herein, a light-field reconstruction method to image the RI map within a depth of 0.2 μm is proposed. It records quantitative phase-delay images using a four-step phase shifting method in different directions and then reconstructs a similar scattered light field for the refractive sample on the focus plane...
August 2015: Micron: the International Research and Review Journal for Microscopy
Feifei Tong, Yan Lian, Fengjiao He
A novel square Au microelectrode multi-channel series piezoelectric quartz crystal (S-MSPQC) cell sensor was constructed by square Au microelectrode in series connected with quartz crystal sensor in MSPQC. The experimental results showed square shape Au microelectrode was more sensitive than normally used interdigital microelectrode. New constructed S-MSPQC was successfully used for real time monitoring the injury of human umbilical vein endothelial cells which induced by lipopolysaccharide (LPS) and assessing the drug effects of two anti-oxidative vitamins, VC and VE and their combination against this injury...
September 15, 2015: Biosensors & Bioelectronics
Yong-Jin Chen, Yin-Hua Zhao, Ya-Juan Zhao, Nan-Xia Liu, Xin Lv, Qiang Li, Fa-Ming Chen, Min Zhang
Our aim is to investigate the cytobiological effects of autologous platelet-rich fibrin (PRF) on dental pulp stem cells (DPSCs) and to explore the ectopic and orthotopic possibilities of dental pulp revascularization and pulp-dentin complex regeneration along the root canal cavities of the tooth by using a novel tissue-engineered transplant composed of cell-sheet fragments of DPSCs and PRF granules. Canine DPSCs were isolated and characterized by assaying their colony-forming ability and by determining their cell surface markers and osteogenic/adipogenic differentiation potential...
August 2015: Cell and Tissue Research
Cong-Cong Zhang, Gang Chen, Jia-Lan Liu, Ye Li, Xiao-Yu Xu
As a novel bioaffinity chromatography technique, cell membrane chromatography (CMC) was first established by Professor He in 1996, with which combined high performance liquid chromatography, cytobiology, and receptor pharmacology. The cell membrane stationary phase (CMSP) consists of porous silica coated with active cell membranes. By immersing silica into a suspension of cell membranes, the whole surface of silica was covered by the cell membranes. In CMC, the interaction of drugs or compounds with the immobilized cell membrane or its receptors is investigated using liquid chromatography...
June 2013: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Qing-Song Liu, Xing-Chun Zhu, Jun-An Li, Yan Xing, Hong Jiang, Jun Zhang, Ming-Hui Yang, Ming-Cai Zhao, Guo-Hua Yuan, Zhong Tang
OBJECTIVE: To investigate the effects of Danshen Injection (DSI) on the proliferation of rheumatoid arthritis fibroblast-like synoviocytes (RA FLSs) cultured in RA patients' serum. METHODS: The RA FLSs harvested from RA patients' synovial fluid were primarily cultured by routines. The cells were cultured with 10% inactivated human serum (the healthy human serum and the RA patients' serum) for 24 h. Then DSI at the final concentration of 0. 4 mg/mL was added in the cells for further 24 h culture...
May 2013: Chinese Journal of Integrated Traditional and Western Medicine
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