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HepG2.2.15

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https://www.readbyqxmd.com/read/29325291/-hepatitis-b-core-antigen-promotes-invasion-of-hepatocellular-carcinoma-cell-line-hepg2-2-15-via-toll-like-receptor-4
#1
H R Hou, Y Kang, Y K Li, Y L Zeng, J F Wei, G G Ding, Z Peng, J Shang
Objective: To investigate the effect of hepatitis B core antigen (HBcAg) in promoting the invasion of hepatitis B virus (HBV)-related hepatocellular carcinoma cell line HepG2.2.15 and the role of Toll-like receptor 4 (TLR4) in the mechanism. Methods: TLR4 mRNA and protein expression in HepG2 cells and HepG2.2.15 cells was measured by reverse transcription real-time PCR and Western blot analysis, respectively. HepG2.2.15 cells were transfected with TLR4 specific small interfering RNA (siRNA) to silence TLR4 expression, and stimulated by recombinant HBcAg in culture...
December 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/29320879/isolation-and-purification-of-diterpenoids-from-the-aerial-parts-of-isodon-excisoides-target-guided-by-uplc-ltq-orbitrap-ms
#2
Li-Ping Dai, Ling-Xia Zhang, Ya-Lin Liu, Hong Wu, Rui-Xin Liu, Meng Zhao, Shuang-Shuang Tian, Xue Jiang, Sui-Qing Chen
Cytotoxic diterpenoids were enriched and orientation prepared from the aerial parts of Isodon excisoides target-guided by UPLC-LTQ-Orbitrap-MS. Four diterpenoids were obtained, including a novel compound: 1α-acetoxy-7α, 14β, 20α-trihydroxy-ent-kaur-16-en-15-one (1); together with three known compounds kamebakaurin (2), lasiokaurin (3), enmenol-1-β-glucoside (4). Their structures were elucidated on the basis of spectroscopic methods in conjunction with published data for their analogues. All compounds were tested for their cytotoxic effects against five human cancer cell lines HCT-116, HepG2, A2780, NCI-H1650 and BGC-823, respectively...
January 10, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29303970/cytotoxic-effects-and-anti-angiogenesis-potential-of-pistachio-pistacia-vera-l-hulls-against-mcf-7-human-breast-cancer-cells
#3
Maryam Seifaddinipour, Reyhaneh Farghadani, Farideh Namvar, Jamaludin Mohamad, Habsah Abdul Kadir
Pistachio (Pistacia vera L.) hulls (PVLH) represents a significant by-product of industrial pistachio processing that contains high amounta of phenolic and flavonoid compounds known to act as antioxidants. The current study was designed to evaluate the anti-tumor and anti-angiogenic potentials of PVLH extracts. The cytotoxic effects of hexane, ethyl acetate, methanol, and water PVLH extracts toward human colon cancer (HT-29 and HCT-116), breast adenocarcinoma (MCF-7), lung adenocarcinoma (H23), liver hepatocellular carcinoma (HepG2), cervical cancer (Ca Ski), and normal fibroblast (BJ-5ta) cells were assessed using a MTT cell viability assay...
January 5, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29280951/cytotoxic-terpenoids-from-the-roots-of-dracocephalum-taliense
#4
Yanyan Deng, Juan Hua, Wenjia Wang, Zhonglang Zhan, Anqi Wang, Shihong Luo
A chemical investigation of methanol extract from the roots of Dracocephalum taliense led to the isolation of a new aromatic abietane diterpenoid, 12-methoxy-18-hydroxy-sugiol (1), and one highly-oxygenated ursane triterpenoid, 2α,3α-dihydroxy-11α,12α-epoxy-urs-28,13β-olide (2), together with 15 known natural products (3-17). Among these, compounds 1-13 and 15-17 were detected for the first time in the genus of Dracocephalum. The structures of all of these isolates were determined by extensively spectroscopic analyses...
December 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29248296/synthesis-and-biological-evaluation-of-4%C3%AE-thiazol-2-yl-amino-4-o-demethyl-4-deoxypodophyllotoxins-as-topoisomerase-ii-inhibitors
#5
Chun-Yan Sang, Heng-Zhi Tian, Yue Chen, Jian-Fei Liu, Shi-Wu Chen, Ling Hui
A series of 4β-(thiazol-2-yl)amino-4'-O-demethyl-4-deoxypodophyllotoxins were synthesized, and their cytotoxicities were evaluated against four human cancer cell lines (A549, HepG2, HeLa, and LOVO cells) and normal human diploid fibroblast line WI-38. Some of the compounds exhibited promising antitumor activity and less toxicity than the anticancer drug etoposide. Among them, compounds 15 and 17 were found to be the most potent synthetic derivatives as topo-II inhibitors, and induced DNA double-strand breaks via the p73/ATM pathway as well as the H2AX phosphorylation in A549 cells...
December 7, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29217749/hepatitis-delta-virus-persists-during-liver-regeneration-and-is-amplified-through-cell-division-both-in-vitro-and-in-vivo
#6
Katja Giersch, Oliver D Bhadra, Tassilo Volz, Lena Allweiss, Kristoffer Riecken, Boris Fehse, Ansgar W Lohse, Joerg Petersen, Camille Sureau, Stephan Urban, Maura Dandri, Marc Lütgehetmann
OBJECTIVE: Hepatitis delta virus (HDV) was shown to persist for weeks in the absence of HBV and for months after liver transplantation, demonstrating the ability of HDV to persevere in quiescent hepatocytes. The aim of the study was to evaluate the impact of cell proliferation on HDV persistence in vitro and in vivo. DESIGN: Genetically labelled human sodium taurocholate cotransporting polypeptide (hNTCP)-transduced human hepatoma(HepG2) cells were infected with HBV/HDV and passaged every 7 days for 100 days in the presence of the entry inhibitor Myrcludex-B...
December 7, 2017: Gut
https://www.readbyqxmd.com/read/29207336/cytotoxicity-molecular-modeling-cell-cycle-arrest-and-apoptotic-induction-induced-by-novel-tetrahydro-1-2-4-triazolo-3-4-a-isoquinoline-chalcones
#7
Magda F Mohamed, Hamdi M Hassaneen, Ismail A Abdelhamid
Novel tetrahydro-[1,2,4]triazolo[3,4-a]isoquinolin-3-yl)-3-arylprop-2-en-1-one derivatives were synthesized and their structures were confirmed by different spectral tools. Cytotoxicity test revealed that some compounds exhibited strong to moderate effect, while others showed weak action against different cancer cell lines (MCF7, A549, HCT116, and Hepg2). Breast carcinoma revealed higher sensitivity toward all derivatives especially compounds 5 and 8 which offered the lowest IC50 values (50.05, and 27.15 μg/ml) respectively, relative to the positive control 5-fluorouracil (5-FU) (IC50 = 178 μg/ml)...
November 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29203143/synthesis-and-bioevaluation-study-of-novel-n-methylpicolinamide-and-thienopyrimidine-derivatives-as-selectivity-c-met-kinase-inhibitors
#8
Linxiao Wang, Shan Xu, Xiuying Chen, Xiaobo Liu, Yongli Duan, Dejia Kong, Dandan Zhao, Pengwu Zheng, Qidong Tang, Wufu Zhu
Four series of N-methylpicolinamide moiety and thienopyrimidine moiety bearing pyridazinone were designed and synthesized and evaluated for the IC50 values against three cancer cell lines (A549, HepG2 and MCF-7) and some selected compounds were further evaluated for the activity against c-Met, Flt-3, VEGFR-2, c-Kit and EGFR kinases. Three compounds (35, 39 and 43) showed more active than positive control Foretinib against A549, HepG2 and MCF-7 cell lines. The most promising compound 43 showed superior activity against A549, HepG2 and MCF-7, with the IC50 values of 0...
November 26, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29199475/chromosomal-instability-and-cytotoxicity-induced-by-ribavirin-comparative-analysis-in-cell-lines-with-different-drug-metabolizing-profiles
#9
Carina Sperotto Librelotto, Daniel Simon, Ana Paula de Souza, Mário Reis Álvares-da-Silva, Rafael Rodrigues Dihl
Ribavirin is an important component of the treatment for hepatitis C virus (HCV) infection and, in combination with the new direct-acting antiviral (DAA) agents, comprises the major current therapeutic regimens. This study evaluated the cytotoxicity and chromosomal instability induced by ribavirin using the in vitro cytokinesis-block micronucleus cytome (CBMN-Cyt) assay in two cell lines with different expression levels of drug-metabolizing enzymes: human hepatocellular carcinoma cells (HepG2) and Chinese hamster ovary (CHO-K1) cells...
December 4, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/29181338/interleukin-35-suppresses-antiviral-immune-response-in-chronic-hepatitis-b-virus-infection
#10
Xue Shao, Jingting Ma, Shengnan Jia, Lanlan Yang, Wudong Wang, Zhenjing Jin
The mechanisms of hepatitis B virus (HBV) persistent infection are not completely understood. Interleukin (IL)-35, which is a newly identified cytokine belongs to IL-12 family, has been demonstrated to induce immunotolerance. Thus, the aim of current study was to investigate the role of IL-35 during chronic HBV infection. A total of 61 patients with chronic HBV infection [37 chronic hepatitis B (CHB) and 24 asymptomatic HBV carriers (ASC)] and 20 healthy individuals were enrolled. IL-35 concentration as well as the modulatory function of IL-35 on CD4+CD25+CD127dim/- regulatory T cells (Tregs) and on HBV antigen-specific CD8+ T cells was investigated...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29180321/-a-recombinant-adenovirus-vector-carrying-murine-interleukin-21-gene-controls-chronic-hbv-infection-in-mice
#11
Xue-Ping Gao, Yang Zhou, Xin-Chun Zheng, Xuan Yi, Li-Bo Tang, Jin-Lin Hou, Yong-Yin Li
OBJECTIVE: To investigate the effect of an adenovirus vector containing murine interleukin-21 gene (Ad-GFP-mIL-21) in virus clearance and on the production of HBV-specific antibodies in mice with persistent HBV infection. METHODS: ELISA and Western blot analysis were used to detect the expression of mIL-21 in the supernatant and cytoplasm of cultured HepG2.2.15 cells after infection by Ad-GFP-mIL-21. Mouse models of chronic HBV infection established by in vivo transduction with rAAV8-1...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29144401/design-synthesis-and-preliminary-biological-evaluation-of-novel-benzyl-sulfoxide-2-indolinone-derivatives-as-anticancer-agents
#12
Lin Tang, Tao Peng, Gang Wang, Xiaoxue Wen, Yunbo Sun, Shouguo Zhang, Shuchen Liu, Lin Wang
In this work, a series of novel benzyl sulfoxide 2-indolinone derivatives was designed and synthesized as potent anticancer agents. Tyrosine kinase inhibitory activity assay indicated that most of the compounds showed significant activity. The in vitro antiproliferative activity of these compounds was further investigated against five human cancer cell lines (HeLa, HepG2, MCF-7, SCC-15, and A549). Several compounds exhibited evident activities. Among them, (Z)-3-(((4-bromobenzyl)sulfinyl)methylene)indolin-2-one (6j) and (Z)-3-((benzylsulfinyl)methylene)-5-bromoindolin-2-one (6o) were found to be effective tyrosine kinase inhibitors (IC50 = 1...
November 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29142435/characterization-of-the-phenolic-compound-gallic-acid-from-sansevieria-roxburghiana-schult-and-schult-f-rhizomes-and-antioxidant-and-cytotoxic-activities-evaluation
#13
Rajalekshmi Maheshwari, Chandrashekara Shastry Shreedhara, Picheswara Rao Polu, Renuka Suresh Managuli, Seena Kanniparambil Xavier, Richard Lobo, Manjunath Setty, Srinivas Mutalik
Background: Sansevieria roxburghiana Schult. and Schult. f. (Asparagaceae) grows in India, Indonesia, Sri Lanka, and tropical Africa. Even though the plant has been traditionally used for the treatment of many ailments, the antioxidant and antiproliferative activities of S. roxburghiana methanol extract and its fractions have not yet been explored. Materials and Methods: Quantitative estimation of phenols and different antioxidant assays were performed using standard methods...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142397/chemical-composition-antimicrobial-and-antitumor-potentiality-of-essential-oil-of-ferula-tingitana-l-apiaceae-grow-in-libya
#14
Waleed Elghwaji, Abeer Mohamed El-Sayed, Kadriya S El-Deeb, Aly M ElSayed
Background: Ferula tingitana L. (Apiaceae) has been considered to have abortive and menstruation-inducing properties. It used to treat sore throat, fever, indigestion, and pains. Objectives: The objective of this study is to establish the chemical composition of the essential oil of flower, leaves of F. tingitana, and to throw light on antimicrobial, cytotoxic activities of Libyan plant. Materials and Methods: The chemical composition of the essential oil of flower (0...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29140705/cornusides-a-o-bioactive-iridoid-glucoside-dimers-from-the-fruit-of-cornus-officinalis
#15
Xian-Sheng Ye, Jun He, Yung-Chi Cheng, Lei Zhang, Hao-Yi Qiao, Xue-Ge Pan, Jia Zhang, Shu-Na Liu, Wei-Ku Zhang, Jie-Kun Xu
Fifteen new and rare iridoid glucoside dimers, cornusides A-O (1-15), and 10 known iridoid glucosides (16-25) were isolated from the fruit of Cornus officinalis. These new chemical structures were established through spectroscopic analysis (UV, IR, HRESIMS, 1D and 2D NMR). Compounds 1-25 were tested for their inhibitory activities by measuring IL-6-induced STAT3 promoter activity in HepG2 cells, and 3, 12, 17, 22, and 23 showed inhibitory effects, with IC50 values of 11.9, 12.2, 14.0, 7.0, and 6.9 μM, respectively...
November 15, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29132372/hepatitis-b-virus-inhibits-the-in-vivo-and-in-vitro-synthesis-and-secretion-of-apolipoprotein-c3
#16
Chengliang Zhu, Hengcheng Zhu, Hui Song, Limin Xu, Longxuan Li, Fang Liu, Xinghui Liu
BACKGROUND: Hepatitis B virus (HBV) infection in the body can damage liver cells and cause disorders in blood lipid metabolism. Apolipoprotein C3 (ApoC3) plays an important role in the regulation of lipid metabolism, but no study on the HBV regulation of ApoC3 has been reported. This purpose of this study was to investigate the effect of HBV on ApoC3 expression and its regulatory mechanism. METHODS: The expression levels of ApoC3 mRNA and protein in the human hepatoma cell lines HepG2 and HepG2...
November 13, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29122633/iridoids-and-sfingolipids-from-hedyotis-diffusa
#17
Changfu Wang, Ping Xin, Youzhi Wang, Xuegang Zhou, Donghua Wei, Chengjie Deng, Shiqin Sun
Seven new compounds were isolated from the aerial part of Hedyotis diffusa, including three iridoid glycosides, hedyoiridoidside A - C (1-3), two cerebrosides, hedyocerenoside F (4) and G (5), and two new ceramides, hedyoceramide A (6) and B (7). And six known iridoid glycosides (8-13) were also obtained. Their structures were established by their physico-chemical constants and spectroscopic analysis. The cytotoxicity of all compounds against tumor cell lines of human cervical cancer HeLa, human leukemia HL-60, human lung cancer A459, human hepatoma HepG2, human gastric gland carcinoma BCG-823, human nasopharyngeal cancer CNE-2, human colon cancer HCT15, and human prostate cancer PC-3 were also evaluated in vitro...
November 6, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29117859/a-novel-chemotherapeutic-sensitivity-testing-system-based-on-collagen-gel-droplet-embedded-3d-culture-methods-for-hepatocellular-carcinoma
#18
Jun Hou, Zhixian Hong, Fan Feng, Yantao Chai, Yunkai Zhang, Qiyu Jiang, Yan Hu, Shunquan Wu, Yingsong Wu, Xunian Gao, Qiong Chen, Yong Wan, Jingfeng Bi, Zheng Zhang
BACKGROUND: Patients suffering from advanced stage hepatocellular carcinoma (HCC) often exhibit a poor prognosis or dismal clinical outcomes due to ineffective chemotherapy or a multi-drug resistance (MDR) process. Thus, it is urgent to develop a new chemotherapeutic sensitivity testing system for HCC treatment. The presence study investigated the potential application of a novel chemotherapeutic sensitivity-testing system based on a collagen gel droplet embedded 3D-culture system (CD-DST)...
November 8, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29116813/tio2-with-tandem-fractionation-taft-an-approach-for-rapid-deep-reproducible-and-high-throughput-phosphoproteome-analysis
#19
Liangliang Ren, Chaoying Li, Wenli Shao, Weiran Lin, Fuchu He, Ying Jiang
Mass-spectrometry-based phosphoproteomic workflows traditionally require efficient prefractionation and enrichment of phosphopeptides to gain an in-depth, global, and unbiased systematic investigation of phosphoproteome. Here we present TiO2 with tandem fractionation (TAFT) approach, which combines titanium dioxide (TiO2) enrichment and tandem high-pH reverse-phase (HpRP) for phosphoproteome analysis in a high-throughput manner; the entire workflow takes only 3 h to complete without laborious phosphopeptide preparation...
November 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29111266/hepatoprotective-and-inhibiting-hbv-effects-of-polysaccharides-from-roots-of-sophora-flavescens
#20
Hua Yang, Zhenhua Zhou, Lifang He, Hao Ma, Wensheng Qu, Jiye Yin, Mengfan Jia, Xiunan Zhao, Junjie Shan, Yueqiu Gao
Roots of Sophora flavescens is an important herbal medicine for treatment of HBV and hepatic carcinoma in China. Alkaloids in the root were well known for exhibiting good hepato-protective and anti-HBV effects. However, polysaccharides as main components in the root remained unknown. In the studies, we investigated the chemical features and hepatoprotective effects of Sophora flavescens polysaccharides (SFP-100 and its active fractions) with ConA-induced hepatitis mice, human liver LO2 cells and HepG2.2.15 cells...
October 27, 2017: International Journal of Biological Macromolecules
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