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HepG2.2.15

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https://www.readbyqxmd.com/read/27867263/interferon-stimulated-gene-15-conjugation-stimulates-hepatitis-b-virus-production-independent-of-type-i-interferon-signaling-pathway-in-vitro
#1
Yujia Li, Shilin Li, Xiaoqiong Duan, Yanzhao Chen, Baihai Jiao, Haiyan Ye, Min Yao, Limin Chen
Hepatitis B virus (HBV) is an important account of infectious hepatitis and interferon (IFN) remains one of the best treatment options. Activation of type I IFN signaling pathway leads to expressions of IFN-stimulated genes (ISGs) which play important roles in antiviral and immunomodulatory responses to HBV or hepatitis C virus (HCV) infection. Our previous studies indicated that ISG15 and its conjugation (ISGylation) were exploited by HCV to benefit its replication and persistent infection. This study was designed to assess the role of ISG15 and ISGylation in HBV infection in vitro...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27862961/identification-of-a-glypican-3-binding-peptide-for-in-vivo-non-invasive-human-hepatocellular-carcinoma-detection
#2
Zainen Qin, Jingjing Wang, Ye Wang, Guohao Wang, Xiangyu Wang, Zhiyang Zhou, Gang Liu, Shi Gao, Lei Zhu
Early and accurate detection of hepatocellular carcinoma (HCC) is essential to improve the prognosis of patients and reduce the morbidity of surgical therapy. Glypican-3 (GPC3) is a protein abnormally expressed in HCC that has been identified as a serological and histochemical HCC marker. A novel peptide that specifically recognizes GPC3 will facilitate early detection of HCC and guide the treatment strategy. Herein, phage display screening technology is utilized to obtain a GPC3 binding peptide (GBP) using HCC cells expressing GPC3 in varying abundances...
November 15, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27853282/novel-podophyllotoxin-derivatives-as-partial-ppar%C3%AE-agonists-and-their-effects-on-insulin-resistance-and-type-2-diabetes
#3
Xiangming Zhang, Huijuan Liu, Bo Sun, Yan Sun, Weilong Zhong, Yanrong Liu, Shuang Chen, Honglei Ling, Lei Zhou, Xiangyan Jing, Yuan Qin, Ting Xiao, Tao Sun, Honggang Zhou, Cheng Yang
Peroxisome proliferator-activated receptor γ (PPARγ) is recognized as a key regulator of insulin resistance. In this study, we searched for novel PPARγ agonists in a library of structurally diverse organic compounds and determined that podophyllotoxin exhibits partial agonist activity toward PPARγ. Eight novel podophyllotoxin-like derivatives were synthesized and assayed for toxicity and functional activity toward PPARγ to reduce the possible systemic toxic effects of podophyllotoxin and to maintain partial agonist activity toward PPARγ...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27809603/solanerioside-a-an-unusual-14-15-dinor-cyclophytane-glucoside-from-the-leaves-of-solanum-erianthum
#4
Shi-Yi Peng, Hang Li, De-Po Yang, Bai Bai, Long-Ping Zhu, Qin Liu, Juan Shen, Zhi-Min Zhao
Solanerioside A (1), the first example of a diterpenoid glucoside featuring a 14,15-dinor-cyclophytane scaffold, together with three known terpene glucosides (2-4) were isolated from the methanol extract of the leaves of Solanum erianthum (Solanaceae). The structure of 1 was mainly characterised on the basis of extensive spectroscopic analyses, especially from the 2D NMR spectra. In addition, the spectroscopic data of (6E, 10E)-5,12-dihydroxy-β-nerolidol 5-O-β-D-glucopyranoside (3) were reported for the first time...
November 4, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27807008/role-of-equilibrative-nucleobase-transporter-1-slc43a3-as-a-ganciclovir-transporter-in-the-induction-of-cytotoxic-effect-of-ganciclovir-in-a-suicide-gene-therapy-with-herpes-simplex-virus-thymidine-kinase
#5
Junji Furukawa, Katsuhisa Inoue, Kinya Ohta, Tomoya Yasujima, Yoshihisa Mimura, Hiroaki Yuasa
A suicide gene therapy using herpes simplex virus thymidine kinase (HSV-TK) with ganciclovir (GCV) has been under development as a tumor-targeted therapy. However, the mechanism of cellular GCV uptake, which is prerequisite in the therapy, has not been clarified. In an attempt to resolve this situation and gain information to optimize HSV-TK/GCV system for cancer therapy, we found that human equilibrative nucleobase transporter 1 (ENBT1) can transport GCV with a Michaelis constant of 2.75 mM in Madin-Darby canine kidney II (MDCKII) cells stably transfected with this transporter...
November 2, 2016: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/27797185/bioactive-pentacyclic-triterpenoids-from-the-leaves-of-cleistocalyx-operculatus
#6
Chen Wang, Ping Wu, Shuai Tian, Jinghua Xue, Liangxiong Xu, Hanxiang Li, Xiaoyi Wei
Thirteen new pentacyclic triterpenoids, cleistocalyxic acids A-K (1, 2, 4, 5, and 7-13) and cleistocalyxolides A (3) and B (6), and 15 known analogues (14-28), based on taraxastane, oleanane, ursane, multiflorane, and lupane skeletons, were isolated from the leaves of Cleistocalyx operculatus. The structures of 1-13 were elucidated by analysis of their spectroscopic data and ECD/TDDFT computations. Cleistocalyxolide A (3), presumed to be derived from the known taraxastane-type compound 14, has a rare rearranged triterpenoid backbone...
October 31, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/27794214/lack-of-evidence-for-a-liver-or-intestinal-mirna-regulation-involved-in-the-hypertriglyceridemic-effect-of-apoc3-3-utr-variant-ssti
#7
Marine Dancer, Cyrielle Caussy, Mathilde Di Filippo, Philippe Moulin, Christophe Marçais, Sybil Charrière
BACKGROUND AND AIMS: APOC3 is a major regulator of triglycerides metabolism. Several APOC3 variants are associated with hypertriglyceridemia (HTG). Our aim was to establish the potential regulation of APOC3 3'UTR variants associated with HTG by liver or intestinal miRNAs. METHODS: We sequenced APOC3 3'UTR in 100 type 2 diabetic (TD2) patients with severe HTG (TG > 15 mmol/L) (HTG group) compared to 100 normotriglyceridemic patients (NTG group). We performed in silico studies to identify potential loss of miRNA binding induced by APOC3 3'UTR variants...
October 13, 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27793933/apoptosis-of-hepatitis-b-virus-expressing-liver-tumor-cells-induced-by-a-high-concentration-of-nucleos-t-ide-analogue
#8
Eunyoung Tak, Shin Hwang, Han Chu Lee, Gi-Young Ko, Chul-Soo Ahn, Young-In Yoon, Young-Suk Lim, Dae-Young Jun, Ki-Hun Kim, Gi-Won Song, Deog-Bok Moon, Baek-Yeol Ryoo, Nayoung Kim, Sung-Gyu Lee
BACKGROUND/AIM: We investigated the expression of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and HBV X protein (HBx) in human hepatocellular carcinoma (HCC) and evaluated the effect of high-concentration nucleos(t)ide analogs (NUCs) on liver tumor cell lines. MATERIALS AND METHODS: This study consisted of three parts: part I used human blood and non-tumor liver tissues; part II used human HCC and adjacent liver tissues; and part III used an HBV-expressing liver tumor cell line...
November 2016: Anticancer Research
https://www.readbyqxmd.com/read/27783362/tissue-transglutaminase-2-exerts-a-tumor-promoting-role-in-hepatitis-b-virus-related-hepatocellular-carcinoma
#9
Chengbo Yu, Qing Cao, Ping Chen, Shigui Yang, Xianli Gong, Min Deng, Bing Ruan, Lanjuan Li
Hepatitis B virus (HBV) infection is a major risk factor which can lead to development of hepatocellular carcinoma (HCC). Tissue transglutaminase-2 (TG2) has been shown to be critical for cancer progression. However, how TG2 promotes the progression of HBV-related HCC remains unknown. In this study, we aimed to explore the expression and function of TG2 on HBV-related HCC progression. The expression levels of TG2 were examined in a series of HBV-related HCC tissues and a panel of HCC cell lines. The effects of TG2 knockdown on the proliferation and migration of HBV-related cells were determined...
October 25, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27779671/simvastatin-exerts-anti-hepatitis-b-virus-activity-by-inhibiting-expression-of-minichromosome-maintenance-protein-7-in-hepg2-2-15-cells
#10
Wenjie Li, Fei Cao, Juan Li, Zhixin Wang, Yu Ren, Zheyong Liang, Peijun Liu
Simvastatin (SIM), a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, has been reported to inhibit the activity of hepatitis B virus (HBV), however, the mechanism underlying its antiviral function remains unknown. Minichromosome maintenance (MCM) 7, a component of the MCM complex, has been reported to act as an important host factor aiding virus genome replication in host cells. The present study demonstrated that downregulation of MCM7 inhibited the expression of proteins transferred by adenoviral vectors...
October 20, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27777009/design-synthesis-and-activity-of-novel-sorafenib-analogues-bearing-chalcone-unit
#11
Min Wang, Shan Xu, Chunjiang Wu, Xiaobo Liu, Hong Tao, Yanli Huang, Yongchan Liu, Pengwu Zheng, Wufu Zhu
Two series of sorafenib derivatives (N-methylpicolinamide-4-oxy) chalcones (5a-o, 7a-e) were synthesized and characterized by NMR and MS. All of the target compounds were evaluated for the cytotoxicity against A549, HepG2, MCF-7, and PC-3 cancer cell lines and some selected compounds were further evaluated for the activity against VEGFR-2/KDR and BRAF kinases. The results indicated that all the compounds showed moderate to good antitumor activity, and the compound 5c showed well cytotoxic activity against HepG2, MCF-7 and PC-3 cell lines with IC50 values of 0...
October 12, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27771945/binding-and-uptake-into-human-hepatocellular-carcinoma-cells-of-peptide-functionalized-gold-nanoparticles
#12
Satadru Jha, Federico Ramadori, Santina Quarta, Alessandra Biasiolo, Enrica Fabris, Paola Baldan, Gaetano Guarino, Mariagrazia Ruvoletto, Gianmarco Villano, Cristian Turato, Angelo Gatta, Fabrizio Mancin, Patrizia Pontisso, Paolo Scrimin
One of the most daunting challenges of nanomedicine is the finding of appropriate targeting agents to deliver suitable payloads precisely to cells affected by malignancies. Even more complex is to achieve the ability to ensure the nanosystems enter those cells. Here we use 2 nm (metal core) gold nanoparticles to target human hepatocellular carcinoma (HepG2) cells stably transfected with the SERPINB3 (SB3) protein. The nanoparticles were coated with a 85:15 mixture of thiols featuring, respectively, a phosphoryl choline, to ensure water solubility and biocompatibility, and a 28-mer peptide corresponding to the amino acid sequence 21-47 of the hepatitis B virus-PreS1 protein (PreS1(21-47))...
October 22, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27769058/bortezomib-resistance-is-associated-with-increased-levels-of-proteasome-subunits-and-apoptosis-avoidance
#13
Yi-Xin Wu, Jia-Hua Yang, Hirotomo Saitsu
Bortezomib (BTZ), a proteasome inhibitor, is the first proteasome inhibitor to be used in clinical practice. Here we investigated the mechanisms underlying acquired bortezomib resistance in hepatocellular carcinoma (HCC) cells. Using stepwise selection, we established two acquired bortezomib-resistant HCC cell lines, a bortezomib-resistant HepG2 cell line (HepG2/BTZ) and bortezomib-resistant HuH7 cell line (HuH7/BTZ). The 50% inhibitory concentration values of HepG2/BTZ and HuH7/BTZ were respectively 15- and 39-fold higher than those of parental cell lines...
October 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27749922/genome-wide-transcriptome-analysis-of-cd36-overexpression-in-hepg2-2-15-cells-to-explore-its-regulatory-role-in-metabolism-and-the-hepatitis-b-virus-life-cycle
#14
Jian Huang, Lei Zhao, Ping Yang, Zhen Chen, Ni Tang, Xiong Z Ruan, Yaxi Chen
Hepatitis B virus (HBV) is a hepatocyte-specific DNA virus whose gene expression and replication are closely associated with hepatic metabolic processes. Thus, a potential anti-viral strategy is to target the host metabolic factors necessary for HBV gene expression and replication. Recent studies revealed that fatty acid translocase CD36 is involved in the replication, assembly, storage, and secretion of certain viruses, such as hepatitis C virus (HCV) and human immunodeficiency virus (HIV). However, the relationship between CD36 and the HBV life cycle remains unclear...
2016: PloS One
https://www.readbyqxmd.com/read/27729742/increased-atg5-atg12-in-hepatitis-b-virus-associated-hepatocellular-carcinoma-and-their-role-in-apoptosis
#15
Areerat Kunanopparat, Ingorn Kimkong, Tanapat Palaga, Pisit Tangkijvanich, Boonchoo Sirichindakul, Nattiya Hirankarn
AIM: To investigate autophagy-related genes, particularly ATG12, in apoptosis and cell cycle in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) and non-HBV-HCC cell lines. METHODS: The expression of autophagy-related genes in HBV-associated hepatocellular carcinoma and non-HBV-HCC cell lines and human liver tissues was examined by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blotting. The silencing of target genes was used to examine the function of various genes in apoptosis and cell cycle progression...
October 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27724895/hepatitis-b-virus-inhibits-apolipoprotein-a5-expression-through-its-core-gene
#16
Chengliang Zhu, Guosheng Gao, Hui Song, Fengxia Xu, Kailang Wu, Xinghui Liu
BACKGROUND: Hepatitis B virus (HBV) infection causes lipid metabolism disorders. Apolipoprotein A5 (ApoA5) is a new apolipoprotein family member that plays an important role in the regulation of lipid metabolism. The present study was to investigate the impact of HBV on ApoA5 expression and its regulatory mechanism. METHODS: Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to measure ApoA5 mRNA and protein expression in HepG2 and HepG2...
October 10, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27706082/extraction-of-natural-antioxidants-from-the-thelephora-ganbajun-mushroom-by-an-ultrasound-assisted-extraction-technique-and-evaluation-of-antiproliferative-activity-of-the-extract-against-human-cancer-cells
#17
Dong-Ping Xu, Jie Zheng, Yue Zhou, Ya Li, Sha Li, Hua-Bin Li
The Thelephora ganbajun mushroom has been found to be a potential rich source of natural antioxidants. In this study, an ultrasound-assisted extraction (UAE) technique together with GRAS (generally recognized as safe) solvents (ethanol and water) was used to maximize the extraction of antioxidants from Thelephora ganbajun. Five extraction parameters (ethanol concentration, solvent to solid ratio, extraction time, temperature and ultrasound power) were investigated by single-factor experiments, and then a central composite rotatable design was employed to study interaction of three key extraction parameters...
October 1, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27698938/optical-imaging-of-mesenchymal-epithelial-transition-factor-met-for-enhanced-detection-and-characterization-of-primary-and-metastatic-hepatic-tumors
#18
Shadi A Esfahani, Pedram Heidari, Sun A Kim, Shuji Ogino, Umar Mahmood
Purpose: To assess optical imaging of Mesenchymal-Epithelial Transition factor (MET) for delineation and characterization of intrahepatic models of human hepatocellular carcinoma (HCC) and metastatic colorectal cancer (CRC), and thereby demonstrate its potential use in precision oncology. Materials and Methods: MET expression in human CRC and HCC was assessed in tissue microarrays. We used GE-137, a modified cyanine 5-tagged peptide for MET targeting. HepG2 and Huh-7 (HCC) and HT-29 (CRC) cells with MET overexpression, and LNCaP cells (negative control) with minimal MET expression were incubated with the probe...
2016: Theranostics
https://www.readbyqxmd.com/read/27671862/plasma-fetuin-a-%C3%AE-2-hs-glycoprotein-correlates-negatively-with-inflammatory-cytokines-chemokines-and-activation-biomarkers-in-individuals-with-type-2-diabetes
#19
Sardar Sindhu, Nadeem Akhter, Steve Shenouda, Ajit Wilson, Rasheed Ahmad
BACKGROUND: Fetuin-A/AHSH is a novel hepatokine that acts as a vascular calcification inhibitor and as an endogenous TLR-4 ligand. Fetuin-A may act as a positive or negative acute phase protein (APP) in disease conditions. The relationship between circulatory fetuin-A and inflammatory biomarkers in type-2 diabetes (T2D) remains controversial. Therefore, the purpose of this study was to determine the plasma fetuin-A levels in 53 T2D (BMI = 29.7 ± 4.5 kg/m(2)) and 72 non-diabetic individuals (BMI = 28...
September 26, 2016: BMC Immunology
https://www.readbyqxmd.com/read/27664977/mir-370-suppresses-hbv-gene-expression-and-replication-by-targeting-nuclear-factor-ia
#20
Hongxia Fan, Ping Lv, Jing Lv, Xiaopei Zhao, Min Liu, Guangling Zhang, Hua Tang
Hepatitis B virus (HBV) infection is a major health problem worldwide. The roles of microRNAs in the regulation of HBV expression are being increasingly recognized. In this study, we found that overexpression of miR-370 suppressed HBV gene expression and replication in Huh7 cells, whereas antisense knockdown of endogenous miR-370 enhanced HBV gene expression and replication in Huh7 cells and HepG2.2.15 cells. Further, we identified the transcription factor nuclear factor IA (NFIA) as a new host target of miR-370...
September 24, 2016: Journal of Medical Virology
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