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HepG2.2.15

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https://www.readbyqxmd.com/read/28161428/growth-differentiation-factor-15-promotes-cell-viability-invasion-migration-and-angiogenesis-in-human-liver-carcinoma-cell-line-hepg2
#1
Liang Wang, Yagang Liu, Wei Li, Zhe Song
AIM: This study was aimed to explore the role of growth differentiation factor 15 (GDF15) in hepatocellular carcinoma (HCC). METHODS: Human liver carcinoma cell line HepG2 was used and transfected with vector and/or short hairpin RNA (shRNA) against GDF15. Then, the transfection efficiency was ascertained by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Cell viability was measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide (MTT)...
February 1, 2017: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/28131852/interaction-of-pharmaceutical-excipients-with-organic-cation-transporters
#2
Sirima Soodvilai, Sunhapas Soodvilai, Varanuj Chatsudthipong, Tanasait Ngawhirunpat, Theerasak Rojanarata, Praneet Opanasopit
Increasing evidences have shown that many pharmaceutical excipients are not pharmacologically inert but instead have effects on several transport function of uptake and efflux drug transporters. Herein, we investigated whether the excipients frequently used in many drug formulations affect transport function of organic cation transporters (OCTs) that are responsible for elimination of cationic drugs. Our finding revealed that solubilizing agents, Tweens, showed the most significant effect rbOCT1/2-mediated [(3)H]-MPP(+) uptake in heterologous expressing cells...
January 25, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28117728/a-new-human-cancer-cell-proliferation-inhibition-sesquiterpene-dryofraterpene-a-from-medicinal-plant-dryopteris-fragrans-l-schott
#3
Zheng-Chang Zhong, Dan-Dan Zhao, Zhen-Dong Liu, Shuai Jiang, Yan-Long Zhang
The global burden of cancer continues to increase largely with the aging and growth of the world population. The purpose of the present work was to find new anticancer molecules from a natural source. We utilized chromatographic methods to isolate compounds from medicinal plant Dryopteris fragrans (L.) Schott. The structure of the new compounds was determined by spectroscopic and spectrometric data (1D NMR, 2D NMR, and EMI-MS). Their anti-proliferation effects against five human cancer cell lines including A549, MCF7, HepG2, HeLa, and PC-3 were evaluated by CCK-8 andlactate dehydrogenase (LDH) assay...
January 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28108382/new-xanthones-and-cytotoxic-constituents-from-garcinia-mangostana-fruit-hulls-against-human-hepatocellular-breast-and-colorectal-cancer-cell-lines
#4
Gamal A Mohamed, Ahmed M Al-Abd, Ali M El-Halawany, Hossam M Abdallah, Sabrin R M Ibrahim
ETHNOPHARMACOLOGICAL RELEVANCE: Cancer has proceeded to surpass one of the most chronic illnesses to be the major cause of mortality in both the developing and developed world. Garcinia mangostana L. (mangosteen, family Guttiferae) known as the queen of fruits, is one of the most popular tropical fruits. It is cultivated in Southeast Asian countries: Malaysia, Indonesia, Sri Lanka, Burma, Thailand, and Philippines. Traditionally, numerous parts of G. mangostana have been utilized to treat various ailments such as abdominal pain, haemorrhoids, food allergies, arthritis, leucorrhoea, gonorrhea, diarrhea, dysentery, wound infection, suppuration, and chronic ulcer...
January 17, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28103869/sinularin-induces-dna-damage-g2-m-phase-arrest-and-apoptosis-in-human-hepatocellular-carcinoma-cells
#5
Ting-Wen Chung, Shih-Chao Lin, Jui-Hsin Su, Yu-Kuo Chen, Chi-Chien Lin, Hong-Lin Chan
BACKGROUND: Sinularin isolated from the cultured soft coral Sinularia flexibilis has been reported to exert potent cytotoxic effects against particular types of cancer. This study was carried out to investigate the cytotoxic effects in sinularin-treated human hepatocellular carcinoma cells, HepG2, and to subsequently explore the underlying molecular mechanisms. METHODS: TheMTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl- tetrazolium bromide) method was used to evaluate the cytotoxicity of sinularin on HepG2 and Hep3B cell lines...
January 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28098861/triptolide-inhibits-viability-and-induces-apoptosis-in-liver-cancer-cells-through-activation-of-the-tumor-suppressor-gene-p53
#6
Yan-Yan Sun, Lei Xiao, Dong Wang, Yan-Chao Ji, Yu-Peng Yang, Rong Ma, Xi-Hai Chen
The present study investigated the effect of triptolide on viability and apoptosis along with underlying mechanism in liver cancer cells. CCK-8 assay showed that triptolide treatment for 48 h significantly reduced the viability of HepG2 and QSG7701 cells at 50 µM concentration. Annexin V-FITC and propidium iodide staining showed that triptolide treatment of HepG2 cells at 50 µM concentrations induced apoptosis in 56.45% cells compared to only 2.36% cells in the control cultures. Western blot assay showed that treatment of HepG2 cells with 50 µM concentration of triptolide significantly induced phosphorylation of p53 in a 2 h-treatment...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28095903/identification-and-application-of-anti-inflammatory-compounds-screening-system-based-on-raw264-7-cells-stably-expressing-nf-%C3%AE%C2%BAb-dependent-seap-reporter-gene
#7
Yue Li, Xiaomeng Wang, Juan Ren, Xi Lan, Jing Li, Jing Yi, Li Liu, Yan Han, Sanqi Zhang, Dongmin Li, Shemin Lu
BACKGROUND: NF-κB is one of the key transcription factors in the inflammatory response, transactivates a series of pro-inflammatory genes and is therefore regarded as an important target for anti-inflammatory drug screening. METHOD: We recombined the reporter gene vector with inserting the "neo" transcript into the vector pNF-κB-SEAP, made the reporter gene vector stable in a eukaryotic cell line. The recombinant reporter gene vector we named pNF-κB-SEAP-Neo was transfected into RAW264...
January 18, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28088184/anti-hepatitis-b-virus-hbv-response-of-imiquimod-based-toll-like-receptor-7-ligand-in-hbv-positive-human-hepatocelluar-carcinoma-cell-line
#8
Dipanwita Das, Isha Sengupta, Neelakshi Sarkar, Ananya Pal, Debraj Saha, Manikankana Bandopadhyay, Chandrima Das, Jimmy Narayan, Shivaram Prasad Singh, Sekhar Chakrabarti, Runu Chakravarty
BACKGROUND: Toll like receptors (TLRs) play an important role in innate immunity and various studies suggest that TLRs play a crucial role in pathogenesis of hepatitis B virus (HBV) infection. The present study aims in looking into the status of crucial host and viral gene expression on inciting TLR7. METHODS: The transcription of TLR7 pathway signaling molecules and HBV DNA viral load were quantified by Real Time-PCR after stimulation of TLR7 with its imiquimod based ligand, R837...
January 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28077172/promoting-effect-of-hepatitis-b-virus-on-the-expressoin-of-phospholipase-a2-group-iia
#9
Chengliang Zhu, Hui Song, Bingzheng Shen, Long Wu, Fang Liu, Xinghui Liu
BACKGROUND: Hepatitis B virus (HBV) infection causes acute and chronic liver disease, ultimately leading to the development of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Phospholipase A2 group IIA (PLA2G2A) plays important roles in the development and progression of many tumors. Thus far, there have been no reports on the association between HBV and PLA2G2A. The present study investigated the effect of HBV infection on PLA2G2A expression and its application in the diagnosis of HBV-related diseases...
January 11, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28073408/-establishment-of-a-cell-co-culture-system-in-accordance-with-the-immunological-characteristics-of-chronic-hbv-infection
#10
H J Li, X Y Yang, F B Kang, F X Liu, D X Sun
Objective: To investigate whether the co-culture of Huh7.93 cells and peripheral blood mononucleated cells from chronic hepatitis B patients (cPBMCs) can simulate the replication features of hepatitis B virus (HBV) and immune function in chronic hepatitis B (CHB) patients, and to provide an in vitro cell co-culture system for the research on immune clearance in chronic HBV infection. Methods: Huh7.93 cells were cultured alone or co-cultured with peripheral blood mononucleated cells from healthy people who underwent physical examination (nPBMCs) or cPBMCs for 7 days...
December 20, 2016: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28061318/hepatitis-b-surface-antigen-promotes-the-invasion-of-hepatitis-b-virus-related-hepatocellular-carcinoma-cells-by-upregulation-of-toll-like-receptor-2
#11
Shan Cheng, Bin Zhang, Ji-Ying Du, Yu-Hong Jin, Hai-Yang Lang, Li-Hua Zeng
Hepatitis B virus (HBV) infection is one of the major risk factors leading to the development of hepatocellular carcinoma (HCC). Hepatitis B surface antigen (HBsAg) plays a pivotal role in HBV-related HCC pathogenesis, and Toll-like receptor (TLR) 2 is also considered to mediate tumor progression. However, the interaction between HBsAg and TLR2 in HCC progression remains unclear. Thus, the aim of the study was to explore the effect of HBsAg-TLR2 pathway on growth and invasion of HBV-related HCC cells and examine the potential mechanisms been involved...
January 6, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28058014/anti-viral-role-of-toll-like-receptor-4-in-hepatitis-b-virus-infection-an-in-vitro-study
#12
Dipanwita Das, Neelakshi Sarkar, Isha Sengupta, Ananya Pal, Debraj Saha, Manikankana Bandopadhyay, Chandrima Das, Jimmy Narayan, Shivram Prasad Singh, Runu Chakravarty
AIM: Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4 (TLR4) in hepatitis B virus (HBV) infection. METHODS: Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the HepG2.2.15 cells with TLR4 specific ligand. Nuclear factor (NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques...
December 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28053323/hbx-promotes-cell-proliferation-by-disturbing-the-cross-talk-between-mir-181a-and-pten
#13
Yi Tian, Xinqiang Xiao, Xing Gong, Feng Peng, Yun Xu, Yongfang Jiang, Guozhong Gong
Hepatitis B virus X protein (HBx) is involved in the initiation and progression of hepatocellular carcinoma (HCC). However, the mechanism is still needed to be elucidated. In this study, we explored the relationship between HBx and microRNA and their roles in hepato-carcinogenesis. Firstly, by global microarray-based microRNA profiling and qRT-PCR, we found miR-181a was strongly up-regulated in HepG2.2.15 cells (HBV positive) and pHBV1.3-expressing HepG2 cells, and HBx played a major role in it. Secondly, reduced PTEN protein expression in the presence of HBx was aslo mediated by miR-181a, and in the Luciferase reporter system, miR-181a inhibited the PTEN translation by binding the PTEN 3'-untranslated-region (UTR), and PTEN protein was decreased when epigenetic expression of miR-181a and rescued by knocking down miR-181a...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28036007/merosesquiterpene-congeners-from-the-australian-sponge-hyrtios-digitatus-as-potential-drug-leads-for-atherosclerosis-disease
#14
Huda A Wahab, Ngoc B Pham, Tengku S Tengku Muhammad, John N A Hooper, Ronald J Quinn
A study of the chemical constituents from the Australian Sponge Hyrtios digitatus has provided a perspective on the connection between the chemistry and biology of the puupehenones, a unique and unusual class of merosesquiterpenes. In this study, a new tetracyclic merosesquiterpene, 19-methoxy-9,15-ene-puupehenol (1) was isolated from the marine sponge Hyrtios digitatus along with the known 20-methoxy-9,15-ene-puupehenol (2). Their structures were elucidated on the basis of spectroscopic data (¹H and (13)C NMR) in combination with experimental electronic circular dichroism (ECD) data...
December 27, 2016: Marine Drugs
https://www.readbyqxmd.com/read/28035986/anti-hbv-activities-of-three-compounds-extracted-and-purified-from-herpetospermum-seeds
#15
Pu-Yang Gong, Zhi-Xiang Yuan, Jian Gu, Rui Tan, Jia-Chuan Li, Yan Ren, Sha Hu
The goal of this research was to evaluate the anti-hepatitis B virus (HBV) activities of three compounds extracted and purified from Herpetospermum seeds (HS) on HepG2.2.15 cells. Herpetin (HPT), herpetone (HPO), and herpetfluorenone (HPF) were isolated from HS and identified using HR-ESI-MS and NMR. Different concentrations of the drugs were added to the HepG2.2.15 cells. Cell toxicity was observed with an MTT assay, cell culture supernatants were collected, and HBsAg and HBeAg were detected by ELISA. The content of HBV DNA was determined via quantitative polymerase chain reaction (PCR) with fluorescent probes...
December 27, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28004399/polymorphisms-in-irg1-gene-associated-with-immune-responses-to-hepatitis-b-vaccination-in-a-chinese-han-population-and-function-to-restrain-the-hbv-life-cycle
#16
Xing Liu, Li Zhang, Xiao-Pan Wu, Xi-Lin Zhu, Li-Ping Pan, Tao Li, Bing-Yu Yan, Ai-Qiang Xu, Hui Li, Ying Liu
Vaccination against the hepatitis B virus (HBV) is extensively used as an effective method to prevent HBV infection. However, nearly 10% of healthy adults fail to produce a protective level of antibodies against the hepatitis B vaccine, and multiple genetic variants are known to affect the immune response to the hepatitis B vaccine. The aim of the present study was to investigate the association between polymorphisms in immunoresponsive gene 1 (IRG1) gene and the immune response to hepatitis B vaccination in a Chinese Han population...
December 22, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27990570/four-mononuclear-platinum-ii-complexes-synthesis-dna-bsa-binding-dna-cleavage-and-cytotoxicity
#17
REVIEW
Qingming Wang, Lei Yang, Jiahui Wu, Hua Wang, Jialiang Song, Xinhui Tang
Four new platinum(II) complexes: Pt(II) L1·H2O (C1, H2 L1 = C20H16N2O2), Pt(II) L2Cl2 (C2, L2 = C22H16N2O2), Pt(II) L3Cl2·H2O (C3, L3 = C20H16N2), Pt(II) L4Cl2·0.4H2O (C4, L4 = C18H14N4) have been synthesized and characterized by using various physico-chemical techniques. The binding interaction of the four platinum(II) complexes C1-C4 with calf thymus (CT)-DNA has been investigated by UV-Vis and fluorescence emission spectrometry. The apparent binding constant (K app) values follow the order: C3 > C1 > C2 > C4...
February 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/27989113/optimization-and-synthesis-of-pyridazinone-derivatives-as-novel-inhibitors-of-hepatitis-b-virus-by-inducing-genome-free-capsid-formation
#18
Dong Lu, Feifei Liu, Weiqiang Xing, Xiankun Tong, Lang Wang, Yajuan Wang, Limin Zeng, Chunlan Feng, Li Yang, Jianping Zuo, Youhong Hu
The capsid of hepatitis B virus (HBV) plays a vital role in virus DNA replication. Targeting nucleocapsid function has been demonstrated as an effective approach for anti-HBV drug development. A high-throughput screening and mechanism study revealed the hit compound 4a as an HBV assembly effector (AEf), which could inhibit HBV replication by inducing the formation of HBV DNA-free capsids. The subsequent SAR study and drug-like optimization resulted in the discovery of the lead candidate 4r, with potent antiviral activity (IC50 = 0...
December 29, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27984167/sesquiterpenes-from-the-whole-plants-of-parasenecio-roborowskii
#19
Xiao-Yan Pang, Yun-Xia Li, Yuan Gong, Yuan Yan, Hong-Fang Li, Ying Zhu
Six eremophilane-type (parasenolide A-F) and an eudesmane-type (parasenin) sesquiterpenoids, along with eight known sesquiterpenes, were isolated from the whole plants of Parasenecio roborowskii. The structures and absolute configurations of new compounds were elucidated using extensive spectroscopic analysis, including HRESIMS, 1D and 2D NMR experiments, the CD exciton chirality methods, and single-crystal X-ray crystallography. All isolated compounds were evaluated for cytotoxicity against five human cancer (HeLa, HepG2, K562, MDA231, and NCI-H460) cell lines and a murine melanoma B16 F10 cell line by MTT assay...
January 2017: Fitoterapia
https://www.readbyqxmd.com/read/27978927/-zinc-finger-e-box-binding-homeobox-2-inhibits-hepatitis-b-virus-replication-and-expression
#20
X P Li, Q Hu, Q He, W X Chen
Objective: To investigate the effect of zinc finger E-box-binding homeobox 2 (ZEB2) on hepatitis B virus (HBV) replication and expression. Methods: HepG2, HepG2.2.15, and HepAD38 cells were cultured separately, and Western blot was used to measure the expression of ZEB2. HepG2.2.15 cells were cultured and transfected with ZEB2 expression plasmids or shRNA targeting ZEB2. Western blot was used to measure the expression of ZEB2 and HBV core proteins, quantitative real-time PCR was used to measure HBV 3.5 kb RNA and HBV DNA, Southern blot was used to measure HBV replicative intermediate, and ELISA was used to measure the expression of HBsAg and HBeAg, in order to clarify the effect of ZEB2 on HBV replication and expression...
November 20, 2016: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
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