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https://www.readbyqxmd.com/read/29775705/effects-of-different-chemical-modifications-on-the-antibacterial-activities-of-polysaccharides-sequentially-extracted-from-peony-seed-dreg
#1
Xiao-Li Li, Kiran Thakur, Yuan-Yuan Zhang, Xiao-Fang Tu, Ying-Shuo Zhang, Dan-Ye Zhu, Jian-Guo Zhang, Zhao-Jun Wei
The peony seed dreg polysaccharide (PSDPs) fractions and their modified derivatives were examined for their antibacterial potential. PSDPs along with their derivatives exhibited appreciable antibacterial activity against both Gram-positive (B. subtilis and S. aureus) as well as Gram-negative (E. coli and S. typhimurium) bacteria. Among the four polysaccharide fractions, HBSS possessed the greatest antibacterial activity with an inhibition zone of 13.6 mm against S. typhimurium, minimal inhibitory concentration of 0...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775695/pulsatilla-decoction-inhibits-candida-albicans-proliferation-and-adhesion-in-a-mouse-model-of-vulvovaginal-candidiasis-via-the-dectin-1-signaling-pathway
#2
Lilin Yang, Haiwang Wu, Weiyu Qiu, Longyi Guo, Xin Du, Qingying Yu, Jie Gao, Songping Luo
ETHNOPHARMACOLOGICAL RELEVANCE: Pulsatilla decoction (PD) is a classical prescription in Traditional Chinese Medicine (TCM) and has been reported to have inhibitory effects on Candida albicans proliferation. STUDY AIM: To investigate the therapeutic effects of PD in the treatment of Vulvovaginal candidiasis (VVC) and elucidate the potential mechanism. MATERIALS AND METHODS: Female BALB/c mice (N=90) were randomized to six treatment groups, including the Control group, Model group, three PD groups and Fluconazole group which served as a positive control (20mg/kg weekly)...
May 15, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29775652/advances-and-prospects-of-bacillus-subtilis-cellular-factories-from-rational-design-to-industrial-applications
#3
REVIEW
Yang Gu, Xianhao Xu, Yaokang Wu, Tengfei Niu, Yanfeng Liu, Jianghua Li, Guocheng Du, Long Liu
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations. Till date, B. subtilis has been identified as attractive hosts for the production of recombinant proteins and chemicals. By applying various systems and synthetic biology tools, the productivity features of B. subtilis can be thoroughly analyzed and further optimized via metabolic engineering...
May 15, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29775296/incorporation-of-a-theranostic-two-tone-luminescent-silver-complex-into-biocompatible-agar-hydrogel-composite-for-the-eradication-of-eskape-pathogens-in-a-skin-and-soft-tissue-infection-model
#4
Miguel N Pinto, Jorge Martinez-Gonzalez, Indranil Chakraborty, Pradip K Mascharak
Microbial invasion and colonization of the skin and underlying soft tissues are among the most common types of infections, becoming increasingly prevalent in hospital settings. Systemic antibiotic chemotherapies are now extremely limited due to emergence of drug-resistant Gram-positive and multidrug-resistant Gram-negative bacterial strains. Topical administration of antimicrobials provides an effective route for the treatment of skin and soft tissue infections (SSTIs). Therefore, the development of new and effective materials for the delivery of these agents is of paramount importance...
May 18, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29774899/smartphone-based-rapid-quantification-of-viable-bacteria-by-single-cell-microdroplet-turbidity-imaging
#5
Xiaonan Cui, Lihui Ren, Yufei Shan, Xixian Wang, Zhenlong Yang, Chunyu Li, Jian Xu, Bo Ma
Standard plate count (SPC) has been recognized as the golden standard for the quantification of viable bacteria. However, SPC usually takes one to several days to grow individual cells into a visible colony, which greatly hampers its application in rapid bacteria enumeration. Here we present a microdroplet turbidity imaging based digital standard plate count (dSPC) method to overcome this hurdle. Instead of cultivating on agar plates, bacteria are encapsulated in monodisperse microdroplets for single-cell cultivation...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29774878/-etiology-and-risk-factors-for-meningitis-and-or-bacteremia-after-craniotomy-for-patients-with-glioma
#6
Yaning Wang, Yu Wang, Wenbin Ma
To evaluate the incidence and microbiological profile of meningitis and/or bacteremia after craniotomy in patients with glioma and analyze the risk factors relevant to postoperative meningitis.
 Methods: All demographic data, etiological data and clinical data for hospitalized patients who underwent craniotomy in Dept. of Neurosurgery of Peking Union Medical College Hospital between Jan. 2013 to Dec. 2017 were recorded.
 Results: The incidence of post-craniotomy meningitiswas 7.73%, bacteremia was 2.21% and meningitis combined with bacteremia was 1...
April 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29774159/toll-like-receptors-2-and-4-modulate-intestinal-il-10-differently-in-ileum-and-colon
#7
Eva Latorre, Elena Layunta, Laura Grasa, Julián Pardo, Santiago García, Ana I Alcalde, José E Mesonero
Background: Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 are immune recognition receptors expressed in the intestinal epithelium, the first physical-physiological barrier for microorganisms, to inform the host of the presence of Gram-positive and Gram-negative organisms. Interleukin (IL)-10 is an essential anti-inflammatory cytokine that contributes to maintenance of intestinal homeostasis...
April 2018: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29773964/building-a-completely-positive-factorization
#8
Immanuel M Bomze
A symmetric matrix of order n is called completely positive if it has a symmetric factorization by means of a rectangular matrix with n columns and no negative entries (a so-called cp factorization ), i.e., if it can be interpreted as a Gram matrix of n directions in the positive orthant of another Euclidean space of possibly different dimension. Finding this factor therefore amounts to angle packing and finding an appropriate embedding dimension. Neither the embedding dimension nor the directions may be unique, and so many cp factorizations of the same given matrix may coexist...
2018: Central European Journal of Operations Research
https://www.readbyqxmd.com/read/29773927/influence-of-meteorological-factors-on-the-level-and-characteristics-of-culturable-bacteria-in-the-air-in-gliwice-upper-silesia-poland
#9
Ewa Brągoszewska, Józef S Pastuszka
Numerous studies have focused on occupational and indoor environments because people spend more than 90% of their time in them. Nevertheless, air is the main source of bacteria in indoors, and outdoor exposure is also crucial. Worldwide studies have indicated that bacterial concentrations vary among different types of outdoor environments, with considerable seasonal variations as well. Conducting comprehensive monitoring of atmospheric aerosol concentrations is very important not only for environmental management but also for the assessment of the health impacts of air pollution...
2018: Aerobiologia
https://www.readbyqxmd.com/read/29773926/comparative-analysis-of-airborne-bacteria-and-fungi-in-two-salt-mines-in-poland
#10
Elżbieta Gębarowska, Wojciech Pusz, Jolanta Kucińska, Włodzimierz Kita
The aim of this work was to determine the genera or species composition and the number of colony forming units of airborne bacteria and fungi, respectively, in two salt mines in Poland "Wieliczka" (Lesser Poland) and "Polkowice-Sieroszowice" (Lower Silesia). Both of them are working environments characterized by extreme conditions, and additionally "Wieliczka," officially placed on the UNESCO World Heritage Sites' list, plays a role of tourist attraction. There are also some curative chambers located in this mine...
2018: Aerobiologia
https://www.readbyqxmd.com/read/29773450/acromioclavicular-joint-septic-arthritis-in-an-immunocompetent-child-a-case-report
#11
Saurabh Dutt, Jeetendra Lodhi, Vinod Kumar, Abhishek Kashyap
Septic arthritis of acromioclavicular (AC) joint is a rare entity. It is generally seen in patients who are immunocompromised. Only 15 cases have been reported till now, with only one case series of 6 patients. We report a case of septic arthritis of AC joint in an immunocompetent child. A 9 years old girl presented with history of pain in left shoulder for 4 days associated with fever. No history suggestive of any immunocompromised state was complained. On local examination, a swelling of around 3 cm in diameter was found over left AC joint region with raised local temperature, tenderness on palpation and positive response in fluctuation test...
March 23, 2018: Chinese Journal of Traumatology, Zhonghua Chuang Shang za Zhi
https://www.readbyqxmd.com/read/29772229/polybacterial-community-analysis-in-human-conjunctiva-through-16s-rrna-gene-libraries
#12
Deepthi KrishnanNair Geetha, Jayasudha Rajagopalaboopathi, Girish Rameshan Nair, Manikandan Palanisamy, Ram Rammohan, Narendran Venkatapathy, Prabagaran Solai Ramatchandirane
The conjunctival sac of healthy human harbours a variety of microorganisms. When the eye is compromised, an occasional inadvertent spread happens to the adjacent tissue, resulting in bacterial ocular infections. Microbiological investigation of the conjunctival swab is one of the broadly used modality to study the aetiological agent of conjunctiva. However, most of the time such methods yield unsatisfactory results. Hence, the present study intends to identify the bacterial community in human conjunctiva of pre-operative subjects through 16S rRNA gene libraries...
May 14, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29771856/vitamin-e-phosphate-coating-stimulates-bone-deposition-in-implant-related-infections-in-a-rat-model
#13
Arianna B Lovati, Marta Bottagisio, Susanna Maraldi, Martina B Violatto, Monica Bortolin, Elena De Vecchi, Paolo Bigini, Lorenzo Drago, Carlo L Romanò
BACKGROUND: Implant-related infections are associated with impaired bone healing and osseointegration. In vitro antiadhesive and antibacterial properties and in vivo antiinflammatory effects protecting against bone loss of various formulations of vitamin E have been demonstrated in animal models. However, to the best of our knowledge, no in vivo studies have demonstrated the synergistic activity of vitamin E in preventing bacterial adhesion to orthopaedic implants, thus supporting the bone-implant integration...
June 2018: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/29771448/segmented-filamentous-bacteria-induced-immune-responses-a-balancing-act-between-host-protection-and-autoimmunity
#14
REVIEW
Kyle L Flannigan, Timothy L Denning
Segmented filamentous bacteria (SFB) are gram-positive, spore-forming, bacteria that primarily colonize the ileum of the small intestine. Upon direct adherence to intestinal epithelial cells, SFB actively stimulate innate and adaptive immune cell activation. The cardinal features of SFB-induced gut immunity - Th17 cell differentiation, IgA production, and barrier protection - lead to the containment of SFB and further afford protection against invading pathogens. Th17 cells and IL-17A, however, can also reach peripheral sites and exacerbate autoimmunity...
May 17, 2018: Immunology
https://www.readbyqxmd.com/read/29770866/meucin-49-a-multifunctional-scorpion-venom-peptide-with-bactericidal-synergy-with-neurotoxins
#15
Bin Gao, Julie Dalziel, Simone Tanzi, Shunyi Zhu
Besides key roles in prey capture and predator defense, scorpion venom also functions as internal immune agents protecting the venom gland from infection and external immune agents cleaning saprophytic microbes from their own body surfaces. However, antimicrobials (typically antimicrobial peptides, AMPs) in the venom often exist in low abundance that might exclude their immune role alone, leaving an open question with regard to their in vivo biological function. Here, we report the bactericidal activity of seven peptides isolated from the scorpion Mesobuthus eupeus venom, including one classical α-helical AMP and five ion channel-targeted neurotoxins...
May 16, 2018: Amino Acids
https://www.readbyqxmd.com/read/29769838/selected-biomarkers-correlate-with-the-origin-and-severity-of-sepsis
#16
Michal Holub, Olga Džupová, Michaela Růžková, Alžběta Stráníková, Eva Bartáková, Jan Máca, Jiří Beneš, Heiko Herwald, Ondřej Beran
The microbial etiology and source of sepsis influence the inflammatory response. Therefore, the plasma levels of cytokines (IL-6, IL-8, and IL-10), chemokines (CCL2/MCP-1, MIP-1 β ), heparin-binding protein (HBP), soluble CD14 (sCD14), and cortisol were analyzed in blood from septic patients obtained during the first 96 hours of intensive care unit hospitalization. The etiology was established in 56 out of a total of 62 patients enrolled in the study. Plasma concentrations of MCP-1, sCD14, IL-6, and IL-10 were significantly higher in patients with community-acquired pneumonia (CAP; n = 10) and infective endocarditis (IE; n = 11) compared to those with bacterial meningitis (BM; n = 18)...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/29769762/polymicrobial-synergy-and-dysbiosis-an-overview
#17
REVIEW
Hawaabi Faqeer Mohd Shaikh, Suvarna Hanmantgouda Patil, Tanvi Shyamsundar Pangam, Khushboo Vijaysinh Rathod
The oral fissure is immensely inhabited with a number of polymicrobial colonies similar to the intestinal system. Periodontitis is a dysbiotic disease resulting from deviation in subgingival Gram-positive bacteria to Gram-negative bacteria shift from Gram-positive bacteria. The development of periodontal dysbiosis occurs over a broadened timeframe, which slowly turns the symbiotic association of host and microbe to pathogenic. This review highlights a recent paradigm of periodontitis progression has been postulated which challenges the traditional concept of periodontitis being induced by few particular periopathogens such as belonging to red complex, but by a more comprehensive dysbiotic-synergistic community...
March 2018: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29769277/rapid-screening-of-urinary-tract-infection-and-discrimination-of-gram-positive-and-gram-negative-bacteria-by-automated-flow-cytometric-analysis-using-sysmex-uf-5000
#18
Seon Young Kim, Yumi Park, Hyunjin Kim, Jimyung Kim, Sun Hoe Koo, Gye Cheol Kwon
Rapid screening of urinary tract infection is important to determine antibiotic treatment and reduce unnecessary urine culture. We evaluated the performance of the new flow cytometry-based UF-5000 automated urine analyzer (Sysmex, Kobe, Japan). A total of 1,430 urine samples from 1,226 patients were analyzed and compared with urine cultures to which a Previ Isola (bioMérieux, Marcy l'Etoile, France) system was applied. In total, 878 of 1,430 urine cultures (61.4%) produced ≥ 103 CFU/mL bacterial growth [lsqb]309 with Gram-negative (GN) bacteria, 517 with Gram-positive (GP) bacteria, and 52 mixed cultures[rsqb], with 336 samples (23...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29769066/phenolic-profile-and-biological-properties-of-the-leaves-of-ficus-vasta-forssk-moraceae-growing-in-egypt
#19
Maria Fernanda Taviano, Khaled Rashed, Angela Filocamo, Francesco Cacciola, Paola Dugo, Luigi Mondello, Carlo Bisignano, Rosaria Acquaviva, Manuela D'Arrigo, Natalizia Miceli
BACKGROUND: Ficus vasta Forssk. (Moraceae) is traditionally used for the treatment of various ailments; nonetheless, this species has been poorly studied to date. This work aimed to characterize the phenolic profile and to evaluate the antioxidant and antimicrobial properties of a hydroalcoholic extract obtained from F. vasta leaves collected in Egypt. METHODS: The phenolic profile of the extract was characterized by HPLC-PDA/ESI-MS. The antioxidant properties were examined by different in vitro systems: DPPH test, reducing power and metal chelating activity assays...
May 16, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29768888/effect-of-surfactants-on-the-structure-and-adsorption-efficiency-of-hydroxyapatite-nanorods
#20
Manisha Sharma, Amit Mishra, Akansha Mehta, Diptiman Choudhury, Soumen Basu
Porous hydroxyapatite (HAp) nanorods using surfactant templating proceeded via microwave irradiation method. Study of BET surface area measurement of the HAp nanorods showed that surface area of HAp nanorods with a mixture of cat-anionic surfactants was higher (56.16 m2/g) than their individual counterpart (for anionic 52.8 m2/g and for cationic 48.8 m2/g) as well as without surfactant (19.07 m2/g) due to higher synergistic effect and low critical aggregation concentration of the mixture. Surfactant-directed synthesis of porous HAp has been explored in literature, but the relation between the pore size distribution, surface area and morphology and choice of surfactant(s) was not fully understood and hence in this work we have explored those parameters...
January 1, 2018: Journal of Nanoscience and Nanotechnology
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