keyword
MENU ▼
Read by QxMD icon Read
search

Gram positive

keyword
https://www.readbyqxmd.com/read/27932288/pressure-ulcer-as-a-reservoir-of-multiresistant-gram-negative-bacilli-risk-factors-for-colonization-and-development-of-bacteremia
#1
Iolanda A Braga, Cristiane S Brito, Augusto Diogo Filho, Paulo P Gontijo Filho, Rosineide M Ribas
The purpose of this study was to identify the risk factors that predispose patients who are hospitalized with pressure ulcers (PUs) colonized by Gram-negative bacilli (GNB) to develop bacteremia. In addition, we also detected main phenotypes of resistance in infected and uninfected PUs. A prospective cohort study was conducted at the Clinical Hospital of the Federal University of Uberlândia including patients with Stage II or greater PUs, colonized or not with GNB, from August 2009 to July 2010. Infected ulcers were defined based on clinical signs and on positive evaluation of smears of wound material translated by a ratio of polymorphonuclear cells to epithelial cells ≥2:1, after Giemsa staining...
December 5, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27932160/successful-short-term-intravenous-treatment-of-disseminated-nocardia-farcinica-infection-with-severe-hyponatremia-after-kidney-transplantation-a-case-report
#2
A L Herzog, C Wanner, K Lopau
BACKGROUND: Nocardia is a genus of gram-positive Actinomycetes that are ubiquitous in decaying organic material, soil, and water. Some Nocardia species can infect humans, mainly by airborne transmission. Several reports describe disseminated infections, which are rare and mostly affect strongly immunocompromised patients because intact T-cell-mediated immunity is the major protective mechanism. CASE REPORT: We report a case of disseminated pulmonary, cerebral, and cutaneous infection with Nocardia farcinica in a 66-year-old kidney transplant recipient treated with low-dose triple immunosuppression...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27931257/oral-microflora-in-preschool-children-attending-a-fluoride-varnish-program-a-cross-sectional-study
#3
Maria Anderson, Margaret Grindefjord, Göran Dahllöf, Gunnar Dahlén, Svante Twetman
BACKGROUND: To compare the oral microflora in preschool children attending a fluoride varnish program with a reference group receiving a standard oral health program without fluoride varnish applications. A second aim was to relate the microbial composition to the caries prevalence. METHODS: Five hundred seven 3-year-old children were enrolled from a cohort of 3403 preschool children taking part in a community based oral health project. Two hundred sixty-three of them had attended caries-preventive program with semi-annual applications of a fluoride varnish since the age of 1 year (test group) while 237 had received standard preventive care (reference group)...
December 8, 2016: BMC Oral Health
https://www.readbyqxmd.com/read/27931193/coagulase-negative-staphylococcal-sepsis-in-neonates-do-we-need-to-adapt-vancomycin-dose-or-target
#4
Helgi Padari, Kersti Oselin, Tõnis Tasa, Tuuli Metsvaht, Krista Lõivukene, Irja Lutsar
BACKGROUND: Despite differences in types of infection and causative organisms, pharmacokinetic-pharmacodynamic (PKPD) targets of vancomycin therapy derived from adult studies are suggested for neonates. We aimed to identify doses needed for the attainment of AUC/MIC > 400 and AUC/MIC > 300 in neonates with sepsis and correlate these targets with recommended doses and treatment outcome. METHODS: Neonates who had Vancomycin therapeutic drug monitoring (TDM) performed between January 1, 2010 and December 31, 2012 were studied...
December 8, 2016: BMC Pediatrics
https://www.readbyqxmd.com/read/27930849/searching-for-a-potential-antibacterial-lead-structure-against-bacterial-biofilms-among-new-naphthoquinone-compounds
#5
Caroline S Moreira, Ana Carolina J A Silva, Juliana S Novais, Agnes Marie Sá Figueiredo, Vítor F Ferreira, David R da Rocha, Helena C Castro
AIMS: The aims of this study were design, synthesis and the evaluation of 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and Gram-positive strains, including MRSA and its biofilm, to probe for potential lead structures METHODS AND RESULTS: Thus, 36 new analogs were prepared with good yields using a simple, fast, operational three-procedure reaction and a thiol addition to an ο-quinone methide using microwave irradiation. All compounds were tested against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 15290, Serratia marcescens ATCC 14756, Klebsiella pneumoniae ATCC 4352, Enterobacter cloacae ATCC 23355, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S...
December 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27930823/cultivable-microbiome-of-fresh-white-button-mushrooms
#6
Werner Rossouw, Lise Korsten
Microbial dynamics on commercially grown white button mushrooms is of importance in terms of food safety assurance and quality control. The purpose of this study was to establish the microbial profile of fresh white button mushrooms. The total microbial load was determined through standard viable counts. Presence and isolation of gram-negative bacteria including coagulase positive Staphylococci were performed using a selective enrichment approach. Dominant and presumptive organisms were confirmed using molecular methods...
December 8, 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27930270/halofilum-ochraceum-gen-nov-sp-nov-a-new-gammaproteobacterium-isolated-from-a-marine-solar-saltern
#7
Jun Xia, Jin-Xin Zhao, Jin Sang, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, oxidase-negative, catalase-positive, facultative anaerobe, designated XJ16T, was isolated from a marine solar saltern on the coast of Weihai, China. Cells of the strain XJ16T were long and rod-shaped. The colonies were ochre in color and were able to reduce nitrate to nitrite. Optimal growth occurred at 33-37 °C (range, 20-45 °C) and in the presence of 8-10 % (w/v) NaCl (range, 2-20 %, w/v). The pH range for growth was found to be 6.5-9.5, with optimum growth at pH 7.5-8.0. A phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain XJ16T was related to the phylum Proteobacteria...
December 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27929801/preparation-of-native-cellulose-agcl-fiber-with-antimicrobial-activity-through-one-step-electrospinning
#8
Shaojun Wang, Xiaomin Zhang, Ting Luo, Jin Zhu, Shengpei Su
The native Cellulose-AgCl fiber have been firstly fabricated by one-step electrospinning of cellulose solution with poly(vinyl pyrrolidone) (PVP) and AgNO3. X-ray diffraction (XRD), Scanning electron microscopy (SEM), Energy dispersive spectrometer (EDS), Thermo-gravimetric analysis (TGA) and Fourier transform infrared (FT-IR) are used to characterize the crystal structure, morphology and composition of cellulose-AgCl nanocomposites. The results of SEM indicate that the size of AgCl in cellulose fiber matrix is able to be adjusted by the addition of Polyvinylpyrrolidone(PVP)...
December 8, 2016: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/27928649/the-diagnostic-value-of-1%C3%A2-%C3%A2-%C3%A2-3-beta-d-glucans-and-galactomannan-assays-in-children-suffering-from-bacteremia-in-pediatric-intensive-care-unit
#9
Fang Zheng, Yelian Gu, Hui Zha, Jun Deng, Zhiquan Zhang
The value of (1 → 3)-beta-D-glucans (BG) and galactomannan (GM) assays on diagnostic invasive fungal infections (IFIs) has been observed in adult ICU and in children with hematological malignancies. Only scant data evaluated non-hematological in pediatric intensive care unit (PICU). Here, we retrospectively analyzed the role of bacterial infection to the reactivity of BG and GM assays in PICU. The results showed that the overall prevalence of bacteremia was 13.8% (65/470). The most common underlying disease was pneumonia (81...
December 7, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27928489/effect-of-sticky-mat-usage-in-control-of-nosocomial-infection-in-motahary-burn-hospital
#10
Mostafa Dahmardehei, Faranak Alinejad, Fereshteh Ansari, Mahnaz Bahramian, Mitra Barati
BACKGROUND AND OBJECTIVES: Infection is the most common cause of death among burnt patients and infection control decrease the rate of mortality. The use of sticky mat can control contamination by preventing the entrance microorganisms into the hospital wards. This study was designed to evaluate the sticky mats effect in reduction of microorganism's entry by personnel shoes to burn intensive care unit (BICU). MATERIALS AND METHODS: This is a simple cross sectional study...
June 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/27928186/implementation-of-a-pharmacist-directed-antimicrobial-stewardship-protocol-utilizing-rapid-diagnostic-testing
#11
Angel Heyerly, Ron Jones, Gordon Bokhart, Mary Shoaff, Douglas Fisher
Background: Rapid identification of gram-positive bacteria and resistance determinants from blood cultures can reduce the time to optimal antibiotic therapy. Objective: This study evaluates the use of technology to rapidly identify gram-positive bacteria in combination with a pharmacist-directed antimicrobial stewardship protocol in a tertiary-care facility. Methods: Rapid diagnostic testing was performed on gram-positive blood cultures. Pharmacists were instructed to notify prescribers of results and recommend appropriate antimicrobial therapy based on targeted treatment chart...
November 2016: Hospital Pharmacy
https://www.readbyqxmd.com/read/27927163/phenotypic-and-molecular-detection-of-the-bla-kpc-gene-in-clinical-isolates-from-inpatients-at-hospitals-in-s%C3%A3-o-luis-ma-brazil
#12
Patricia Cristina Saldanha Ribeiro, Andrea Souza Monteiro, Sirlei Garcia Marques, Sílvio Gomes Monteiro, Valério Monteiro-Neto, Martina Márcia Melo Coqueiro, Ana Cláudia Garcia Marques, Rosimary de Jesus Gomes Turri, Simone Gonçalves Santos, Maria Rosa Quaresma Bomfim
BACKGROUND: Bacteria that produce Klebsiella pneumoniae carbapenemases (KPCs) are resistant to broad-spectrum β-lactam antibiotics. The objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in São Luis (Maranhão, Brazil). METHODS: The study was conducted using phenotypic tests and molecular methods, including polymerase chain reaction (PCR), sequencing and enterobacterial repetitive intergenic consensus (ERIC)-PCR...
December 7, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27926821/algoriphagus-aestuariicola-sp-nov-isolated-from-estuary-sediment
#13
Xiaomeng Jia, Baolei Jia, Kyung Hyun Kim, Che Ok Jeon
A Gram-staining-negative and strictly aerobic bacterium, designated strain S2-A1T, was isolated from estuary sediment in South Korea. Cells of strain S2-A1T were oxidase- and catalase-positive rods without a gliding motility. Growth was observed at 15-40 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum, pH 7.0-7.5) and in the presence of 0-4.0 % (w/v) NaCl (optimum, 0.5-1.0 %). The sole respiratory quinone was MK-7. Iso-C15:0, summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c) and summed feature 9 (comprising iso-C17:1 ω9c/C16:0 10-methyl) were found as the major fatty acids (>5 % of the total fatty acids)...
December 7, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27925693/identification-of-n-arylated-nh125-analogues-as-rapid-eradicating-agents-against-mrsa-persister-cells-and-potent-biofilm-killers-of-gram-positive-pathogens
#14
Yasmeen Abouelhassan, Akash Basak, Hussain Yousaf, Robert William Huigens Iii
Bacterial biofilms housing dormant persister cells are innately tolerant to antibiotics and disinfectants, yet several membrane-active agents are known to eradicate tolerant bacterial cells. NH125, a membrane-active persister-killer and starting point for development, led to the identification of N-arylated analogues 1 and 2 and displayed improved biofilm-killing potencies and rapid persister-killing activities in stationary cultures of methicillin-resistant Staphylococcus aureus (MRSA). We found 1 and 2 to be superior to other membrane-active agents in biofilm eradication assays with 1 demonstrating minimum biofilm eradication concentrations (MBEC) of 23...
December 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27924986/a-nasba-on-microgel-tethered-molecular-beacon-microarray-for-real-time-microbial-molecular-diagnostics
#15
Y Ma, X Dai, T Hong, G B Munk, M Libera
Despite their many advantages and successes, molecular beacon (MB) hybridization probes have not been extensively used in microarray formats because of the complicating probe-substrate interactions that increase the background intensity. We have previously shown that tethering to surface-patterned microgels is an effective means for localizing MB probes to specific surface locations in a microarray format while simultaneously maintaining them in as water-like an environment as possible and minimizing probe-surface interactions...
December 7, 2016: Analyst
https://www.readbyqxmd.com/read/27924934/one-pot-synthesis-of-class-ii-lanthipeptide-bovicin-hj50-via-an-engineered-lanthipeptide-synthetase
#16
Jian Wang, Xiaoxuan Ge, Li Zhang, Kunling Teng, Jin Zhong
Lanthipeptides are a large class of bacteria-produced, ribosomally-synthesized and post-translationally modified peptides. They are recognized as peptide antibiotics because most of them exhibit potent antimicrobial activities against Gram-positive bacteria especially those that are phylogenetically related to producers. Maturation of class II lanthipeptide like bovicin HJ50 undergoes precursor modification by LanM and a subsequent leader peptide cleavage by LanT. Herein, via co-expression of precursor gene bovA, modification gene bovM and transporter gene bovT in Escherichia coli C43 (DE3), bioactive bovicin HJ50 was successfully produced and secreted...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924498/isolation-and-characterization-of-acrylamidase-from-arthrobacter-sp-dbv1-and-its-ability-to-biodegrade-acrylamide
#17
Dattatray K Bedade, Rekha S Singhal
Although acrylamide finds diverse industrial applications, its presence in the environment is hazardous due to its carcinogenic, neurotoxic, and teratogenic properties. In spite of the general toxicity of acrylamide in the monomer form, some microorganisms are able to use it as a source of energy by catabolizing it to ammonia and acrylic acid by means of acrylamidase (EC 3.5.1.4). The present work reports on a novel soil isolate as an acrylamide-degrading bacteria. Based on biochemical characterization and 16S ribosomal RNA (rRNA) gene sequence, the bacterial strain was identified as Gram-positive Arthrobacter sp...
December 6, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27923606/blautia-massiliensis-sp-nov-isolated-from-a-fresh-human-fecal-sample-and-emended-description-of-the-genus-blautia
#18
Guillaume A Durand, Thao Pham, Sokhna Ndongo, Sory Traore, Grégory Dubourg, Jean-Christophe Lagier, Caroline Michelle, Nicholas Armstrong, Pierre-Edouard Fournier, Didier Raoult, Matthieu Million
The strain GD9(T) is the type strain of the newly proposed species Blautia massiliensis sp. nov., belonging to the family Lachnospiraceae. It was isolated from a fresh stool sample collected from a healthy human using the culturomics strategy. Cells are Gram-negative rods, oxygen intolerant, non-motile and non-spore forming. The 16S rRNA gene sequencing showed that strain GD9(T) was closely related to Blautia luti, with a 97.8% sequence similarity. Major fatty acids were C14:0 (19.8%) and C16:0 (53.2%). Strain GD9(T) exhibits a genome of 3,717,339 bp that contains 3346 protein-coding genes and 81 RNAs genes including 63 tRNAs...
December 3, 2016: Anaerobe
https://www.readbyqxmd.com/read/27923346/comparison-of-gram-negative-and-gram-positive-hematogenous-pyogenic-spondylodiscitis-clinical-characteristics-and-outcomes-of-treatment
#19
Ching-Yu Lee, Meng-Huang Wu, Chin-Chang Cheng, Tsung-Jen Huang, Tsung-Yu Huang, Chien-Yin Lee, Jou-Chen Huang, Yen-Yao Li
BACKGROUND: To the best of our knowledge, no study has compared gram-negative bacillary hematogenous pyogenic spondylodiscitis (GNB-HPS) with gram-positive coccal hematogenous pyogenic spondylodiscitis (GPC-HPS) regarding their clinical characteristics and outcomes. METHODS: From January 2003 to January 2013, 54 patients who underwent combined antibiotic and surgical therapy in the treatment of hematogenous pyogenic spondylodiscitis were included. RESULTS: Compared with 37 GPC-HPS patients, the 17 GNB-HPS patients were more often found to be older individuals, a history of cancer, and a previous history of symptomatic urinary tract infection...
December 6, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27922772/sarcina-ventriculi-review-of-the-literature
#20
Mohamed Rizwan Haroon Al Rasheed, Carmencita G Senseng
Sarcina ventriculi is an increasingly common gram-positive coccus, recognized in gastric biopsies, particularly of patients with delayed gastric emptying. It occurs most commonly in adult women and can be identified easily by its characteristic morphologic features, such as basophilic staining, cuboid shape, tetrad arrangement, red blood cell-sized packets, flattened cell walls, and refractile nature on light microscopy. Although the pathogenesis of the organism is debated, it has been implicated in cases of gastric perforation, emphysematous gastritis, and peritonitis as well as occurring in the background of gastric adenocarcinomas...
December 2016: Archives of Pathology & Laboratory Medicine
keyword
keyword
105453
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"