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https://www.readbyqxmd.com/read/28432878/in-vitro-susceptibility-of-methicillin-resistant-staphylococcus-aureus-isolates-from-skin-and-soft-tissue-infections-to-vancomycin-daptomycin-linezolid-and-tedizolid
#1
Johanna Marcela Vanegas Múnera, Ana María Ocampo Ríos, Daniela Montoya Urrego, Judy Natalia Jiménez Quiceno
INTRODUCTION: Treatment of multidrug-resistant Gram-positive infections caused by Staphylococcus aureus remains as a clinical challenge due to emergence of new resistance mechanisms. Tedizolid is a next-generation oxazolidinone, recently approved for skin and soft tissues infections. We conducted a study to determine in vitro susceptibility to vancomycin, daptomycin, linezolid, and tedizolid in MRSA clinical isolates from adult patients with skin and soft tissue infections. MATERIAL AND METHODS: Methicillin-resistant S...
April 19, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28432320/formation-of-cuo-nano-flowered-surfaces-via-submerged-photo-synthesis-of-crystallites-and-their-antimicrobial-activity
#2
Fumika Nishino, Melbert Jeem, Lihua Zhang, Kazumasa Okamoto, Satoshi Okabe, Seiichi Watanabe
We report the fabrication of flower-like CuO nanostructured surfaces via submerged photo-synthesis of crystallites (SPSC), which requires only UV illumination in neutral water. In this paper, we discuss the reaction mechanism of the photochemical formation of the SPSC-fabricated CuO nanostructures in detail based on surface microstructural analyses and a radiation-chemical consideration with additional gamma-ray irradiation. Since the SPSC method for surface nanostructural fabrication can work at low temperatures at atmospheric pressure without using harmful substances, it is a potential fabrication method for green nanotechnology applications...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432092/a-genome-wide-investigation-on-biofilm-formation-in-bacillus-cereus
#3
Fang Yan, Yiyang Yu, Kevin Gozzi, Yun Chen, Jian-Hua Guo, Yunrong Chai
Bacillus cereus is a soil-dwelling Gram-positive bacterium capable of forming structured multicellular communities, or biofilms. However, the regulatory pathways controlling biofilm formation are less well understood in B. cereus In this work, we developed a method to study B. cereus biofilms formed at the air-liquid interface. We applied two genome-wide approaches, random transposon insertion mutagenesis to identify genes that are potentially important for biofilm formation, and transcriptome analyses by RNA-seq to characterize genes that are differentially expressed in B...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28430942/short-term-responses-and-resistance-of-soil-microbial-community-structure-to-elevated-co2-and-n-addition-in-grassland-mesocosms
#4
Marie Simonin, Naoise Nunan, Juliette M G Bloor, Valérie Pouteau, Audrey Niboyet
Nitrogen (N) addition is known to affect soil microbial communities, but the interactive effects of N addition with other drivers of global change remain unclear. The impacts of multiple global changes on the structure of microbial communities may be mediated by specific microbial groups with different life-history strategies. Here, we investigated the combined effects of elevated CO2 and N addition on soil microbial communities using PLFA profiling in a short-term grassland mesocosm experiment. We also examined the linkages between the relative abundance of r- and K-strategist microorganisms and resistance of the microbial community structure to experimental treatments...
April 18, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28430277/rapid-single-cell-detection-and-identification-of-pathogens-by-using-surface-enhanced-raman-spectroscopy
#5
N E Dina, H Zhou, A Colniţă, N Leopold, T Szoke-Nagy, C Coman, C Haisch
For the successful treatment of infections, real-time analysis and enhanced multiplex capacity, sensitivity and cost-effectiveness of the developed detection method are critical. In this work, surface-enhanced Raman scattering (SERS) was employed with the final aim of identification and discrimination of pathogenic bacteria, based on their detected SERS fingerprint at the single-cell level. Several genera of bacteria that are found in most of the isolated infections in bacteraemia were successfully identified in less than 5 minutes without the use of antibodies or other specific receptors...
April 21, 2017: Analyst
https://www.readbyqxmd.com/read/28429192/photoinactivation-effect-of-eosin-methylene-blue-and-chlorophyllin-sodium-copper-against-staphylococcus-aureus-and-escherichia-coli
#6
Cynthia S A Caires, Cassia R B Leal, Carlos A N Ramos, Danielle Bogo, Alessandra R Lima, Eduardo J Arruda, Samuel L Oliveira, Anderson R L Caires, Valter A Nascimento
The use of eosin methylene blue according to Giemsa as photosensitizer is presented for the first time in this paper. The present study evaluated the potential application of chlorophyllin sodium copper salt (CuChlNa) and eosin methylene blue according to Giemsa (EMB) as antimicrobial photosensitizers (aPS) for photodynamic inactivation (PDI) of Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. The experiments were performed using S. aureus stain ATCC 25923 and E. coli ATCC 25922 in which five aPS concentrations (0...
April 20, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28429167/corynebacterium-defluvii-sp-nov-isolated-from-sewage
#7
Qiu-Li Yu, Zheng-Fei Yan, Xin He, Feng-Hua Tian, Chuan-Wen Jia, Chang-Tian Li
A Gram-positive, aerobic, non-motile, rod-shapeds, cata-lase-positive, and oxidase-negative strain, designated Y49(T), was isolated from sewage collected from Jilin Agricultural University, China. It grew at 20-40°C (optimum at 30°C), at pH 6.0-8.0 (optimum at 7.0) and at 0-1.0% sodium chloride (optimum at 0%). The major isoprenoid quinone was menaquinone-8 (MK-8) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, four unidentified lipids, and two unidentified aminolipids...
April 20, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28428684/innate-immunity-of-adipose-tissue-in-rodent-models-of-local-and-systemic-staphylococcus-aureus-infection
#8
Andreas Schmid, Thomas Karrasch, Miriam Thomalla, Jutta Schlegel, Bernd Salzberger, Andreas Schäffler, Frank Hanses
Background. The role of adipose tissue in systemic inflammation during bacterial infection is unclear. Effects of Staphylococcus aureus infection on adipocytes in rodent models of experimental endocarditis and peritonitis, the impact of S. aureus infection on gene expression in epididymal and subcutaneous adipose tissue, and effects of S. aureus infection on the toll-like receptor-2- (TLR2-) cathelicidin pathway in vivo and in vitro were investigated. Material and methods. The rat model of catheter-induced S...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28428678/productive-cough-in-children-and-adolescents-view-from-primary-health-care-system
#9
REVIEW
Edin Begic, Zijo Begic, Amra Dobraca, Edo Hasanbegovic
Cough is one of the most common symptoms that doctor faces in working with pediatric population, and according to some characteristics of cough, doctors can often conclude localization, and sometimes even the nature of the disease that causes it. Cough is not only the physiological defense reaction, but a symptom of a disease. According to duration it can be acute, chronic and recidivist, recurrent and persistent, strong or discreet, caused by changes in body position and changes in outside temperature. Pathoanatomically it is divided into lobar, lobular, alveolar and interstitial, pathogenetically to bronchogenic and hematogenous, as well as in immuno competent and immunocompromised, and clinically on the local and inpatient (72 hours after hospital admission)...
February 2017: Medical Archives
https://www.readbyqxmd.com/read/28428342/lysibodies-are-igg-fc-fusions-with-lysin-binding-domains-targeting-staphylococcus-aureus-wall-carbohydrates-for-effective-phagocytosis
#10
Assaf Raz, Anna Serrano, Christine Lawson, Maneesha Thaker, Tricia Alston, Stylianos Bournazos, Jeffrey V Ravetch, Vincent A Fischetti
The cell wall of Gram-positive bacteria contains abundant surface-exposed carbohydrate molecules that are highly conserved within and often across species. The potential therapeutic usefulness of high-affinity antibodies to cell wall carbohydrates is unquestioned, however obtaining such antibodies is challenging due to the poor overall immunogenicity of these bacterial targets. Autolysins and phage lysins are peptidoglycan hydrolases, enzymes that have evolved over a billion years to degrade bacterial cell wall...
April 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28428300/draft-genome-sequence-of-enterococcus-casseliflavus-pavet15-obtained-from-the-oviduct-infection-of-the-cattle-tick-rhipicephalus-microplus-in-jiutepec-morelos-mexico
#11
R Cossío-Bayúgar, E Miranda-Miranda, C A Arreguín-Pérez, L Lozano, D Peréz de la Rosa, M K Rocha-Martínez, M A Bravo-Díaz, B Sachman-Ruiz
Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N50 of 179,476 bp, and 41.93% G+C content.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28428298/genome-sequence-of-staphylococcus-saprophyticus-dpc5671-a-strain-isolated-from-cheddar-cheese
#12
Andrea S Bertuzzi, Caitriona M Guinane, Fiona Crispie, Kieran N Kilcawley, Paul L H McSweeney, Mary C Rea
The draft genome sequence of Staphylococcus saprophyticus DPC5671, isolated from cheddar cheese, was determined. S. saprophyticus is a common Gram-positive bacterium detected on the surface of smear-ripened cheese and other fermented foods.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28427133/-effects-of-application-of-vancomycin-in-the-early-stage-of-patients-with-extremely-severe-burn
#13
Z Zhu, G W Cao, J J Bao, Z H Hu, Z Shen, H Tao, B Cao, F Xu
Objective: To evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. Methods: Data of 15 patients of Kunshan explosion on August 2nd, 2014, admitted to the Department of Intensive Care in our hospital were retrospectively analyzed. The clinical efficacy of continuously intravenous dripping of vancomycin (combined with imipenem) in the early stage of burns (before and on post burn day 14) was analyzed...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28426936/cholesterol-assisted-bacterial-cell-surface-engineering-for-photodynamic-inactivation-of-gram-positive-and-gram-negative-bacteria
#14
Hao-Ran Jia, Ya-Xuan Zhu, Zhan Chen, Fu-Gen Wu
Antibacterial photodynamic therapy (PDT) which enables effective killing of regular and multidrug-resistant (MDR) bacteria is a promising treatment modality for bacterial infection. However, because most photosensitizer (PS) molecules fail to strongly interact with the surface of Gram-negative bacteria, this technique is only suitable for treating Gram-positive bacterial infection, which largely hampers its practical applications. Herein, we revealed for the first time that cholesterol could significantly facilitate the hydrophobic binding of PSs to the bacterial surface, achieving the hydrophobic interaction-based bacterial cell surface engineering that could effectively photo-inactivate both Gram-negative and Gram-positive bacteria...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28426232/glycine-substitution-reduces-antimicrobial-activity-and-helical-stretch-of-dipgla-h-in-lipid-micelles
#15
Marc-Antoine Sani, Catharina Saenger, Davor Juretic, Frances Separovic
With the rise in antibiotic resistance, antimicrobial peptides (AMPs) show promise for therapeutic development but higher specificity is required. PGLa-H is a naturally occurring decapeptide, reported to have moderate antibacterial activity and low hemolytic activity, with its sequence being identical to the C-terminal fragment of highly selective AMP, PGLa. DiPGLa-H, a sequential tandem repeat of PGLa-H, as well as Kiadin, an analogue with a Val to Gly substitution at position 15, display improved in vitro bactericidal activity against both Gram-negative and Gram-positive pathogens, with generally low toxicity for human cells...
April 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28426165/hydrogen-peroxide-dependent-antibacterial-action-of-melilotus-albus-honey
#16
Patrycja Sowa, Dorota Grabek-Lejko, Monika Wesołowska, Sylwia Swacha, Małgorzata Dżugan
Honey originating from different floral sources exhibits the broad-spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as non peroxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennal -3 and annual-5, Melilotus officinalis -1) were collected directly from beekeepers and analyzed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolics contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp...
April 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28426126/actinomyces-in-pregnancy-a-review-of-the-literature
#17
Sarah M Estrada, Everett F Magann, Peter G Napolitano
Importance: Actinomyces is commonly found in many areas of the body where it derives a benefit without harming the host. When it does infect the host during pregnancy, is that infection a threat to the obstetric patient and does that infection cause adverse pregnancy outcomes? Objective: The aim of this study was to review what is known about Actinomyces infections and the impact of an Actinomyces infection on pregnancy outcomes. Evidence Acquisition: A PubMed search was undertaken with the search years unlimited to April 1, 2016, and restricted to articles in English...
April 2017: Obstetrical & Gynecological Survey
https://www.readbyqxmd.com/read/28425874/in-vitro-and-in-vivo-assessments-of-rhodomyrtus-tomentosa-leaf-extract-as-an-alternative-anti-streptococcal-agent-in-nile-tilapia-oreochromis-niloticus-l
#18
Pinanong Na-Phatthalung, Sasitorn Chusri, Naraid Suanyuk, Supayang Piyawan Voravuthikunchai
PURPOSE: Rhodomyrtustomentosa is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of R. tomentosa leaf extract against Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia. METHODOLOGY: The anti-streptococcal activity of R. tomentosa was determined using broth microdilution assays...
April 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28425464/metabolic-labelling-of-the-carbohydrate-core-in-bacterial-peptidoglycan-and-its-applications
#19
Hai Liang, Kristen E DeMeester, Ching-Wen Hou, Michelle A Parent, Jeffrey L Caplan, Catherine L Grimes
Bacterial cells are surrounded by a polymer known as peptidoglycan (PG), which protects the cell from changes in osmotic pressure and small molecule insults. A component of this material, N-acetyl-muramic acid (NAM), serves as a core structural element for innate immune recognition of PG fragments. We report the synthesis of modifiable NAM carbohydrate derivatives and the installation of these building blocks into the backbone of Gram-positive and Gram-negative bacterial PG utilizing metabolic cell wall recycling and biosynthetic machineries...
April 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28425257/role-of-hygienic-factors-in-the-etiology-of-allergic-disorders-in-children
#20
Şule Yeşil, Ahmet Kan, Olkar Abdulmajed, Arzu Bakirtaş, Nedim Sultan, Sadık M Demirsoy
BACKGROUND/AIM: We investigated the role of body flora and chronic inflammatory infections in the etiology of allergic disorders in Turkish children. MATERIALS AND METHODS: Forty pediatric asthma patients with positive skin prick tests and 40 age-matched healthy subjects with negative skin prick tests were enrolled in this cross-sectional study. Serum H. pylori IgG, viral hepatitis serology, IL-10, and TGF-beta levels were measured. Stool and throat cultures were taken and tested for occurrence of microorganisms...
April 18, 2017: Turkish Journal of Medical Sciences
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