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Gram positive

S K Jesudoss, J Judith Vijaya, L John Kennedy, P Iyyappa Rajan, Hamad A Al-Lohedan, R Jothi Ramalingam, K Kaviyarasu, M Bououdina
The present work describes the successful synthesize of spinel magnetic ferrite Mn1-xNixFe2O4 (x=0.0, 0.1, 0.2, 0.3, 0.4 & 0.5) nanoparticles via a simple microwave combustion method which was then evaluated for its photocatalytic activity in the degradation of indigo carmine (IC) synthetic dye, a major water pollutant. Our results reveal that the synthesized of Ni(2+) doped MnFe2O4 nanoparticles possess well-crystalline pure cubic spinel phase, exhibit excellent optical and magnetic properties. Further, the photocatalytic performance of the synthesized nanoparticles at different concentration ratios of Ni(2+) ions was monitored by photocatalytic degradation of indigo carmine synthetic dye under UV (λ=365nm) light irradiation...
October 8, 2016: Journal of Photochemistry and Photobiology. B, Biology
Hatice Tohma, Ekrem Köksal, Ömer Kılıç, Yusuf Alan, Mustafa Abdullah Yılmaz, İlhami Gülçin, Ercan Bursal, Saleh H Alwasel
The identification and quantification of the phenolic contents of methanolic extracts of three Salvia L. species namely S. brachyantha (Bordz.) Pobed, S. aethiopis L., and S. microstegia Boiss. and Bal. were evaluated using reverse phase high performance liquid chromatography, UV adsorption, and mass spectrometry (RP-HPLC/MS). In order to determine the antioxidant capacity of these species, cupric ions (Cu(2+)) reducing assay (CUPRAC) and ferric ions (Fe(3+)) reducing assay (FRAP) were performed to screen the reducing capacity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed for evaluation of the radical scavenging activity for both solvents...
October 21, 2016: Antioxidants (Basel, Switzerland)
Jessica M Andriolo, Richard J Rossi, Casey A McConnell, Baili I Connors, Kevin Trout, M K Hailer, Marisa L Pedulla, Jack L Skinner
The Centers for Disease Control and Prevention have estimated that each year, two million people in the United States become infected with antibiotic-resistant bacteria, of which, approximately 23 000 die as a direct result of these infections. Phage therapy, or the treatment of bacterial infection by specific, antagonistic viruses, provides one alternative to traditional antibiotics. Bacteriophages, or phages, are bacteriaspecific viruses that possess biological traits that allow for not only the removal of bacterial infection, but also the evasion of bacterial resistance which renders antibiotics ineffective...
October 19, 2016: IEEE Transactions on Nanobioscience
Zhao-Qing Du, Li-Na Zhang, Qiang Lu, Yi-Fan Ren, Yi Lv, Xue-Min Liu, Xu-Feng Zhang
The standard therapeutic protocols of pyogenic liver abscess (PLA) have not been well established yet. We investigated the clinical characteristics, disease progression, choices of treatments and outcomes of PLA with different size. 410 cases of patients with PLA were enrolled retrospectively from 2000 to 2014, and were grouped as small abscess (≤5 cm, n = 125), large abscess (5 cm to 10 cm, n = 218) and giant abscess (>10 cm, n = 36). The most common bacteria were Klebsiella pneumonia (22%) and Escherichia coli (11%) by pus culture, and Escherichia coli (36...
October 24, 2016: Scientific Reports
Lorena Rodríguez-Rubio, Wai-Ling Chang, Diana Gutiérrez, Rob Lavigne, Beatriz Martínez, Ana Rodríguez, Sander K Govers, Abram Aertsen, Christine Hirl, Manfred Biebl, Yves Briers, Pilar García
Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations...
October 24, 2016: Scientific Reports
Amna K E Awadallah, Makarim Elfadil M Osman, Mariam A Ibrahim, Emerson S Bernardes, Marcelo Dias-Baruffi, Emadeldin Hassan E Konozy
Three isolectins denoted hereforth MBaL-30, MBaL-60, and MBaL-80 were isolated from seeds extract of Momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. The native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kDa, respectively. On SDS-PAGE, under reduced condition, 27 kDa band was obtained for all isolectins. The lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations = 12.5mM, 50mM, and 0...
October 24, 2016: Journal of Molecular Recognition: JMR
J González-Nava, K Sánchez-Herrera, N Ramírez-Durán, H Sandoval
Nocardia species are aerobic, Gram-positive bacteria with branched filaments reported as opportunistic microorganisms associated with infectious diseases of the skin. We report the isolation of N. wallacei in Mexico from a 43-year-old man, an HIV-positive construction worker who sought care for difficulty breathing and abundant sputum.
November 2016: New Microbes and New Infections
Travis E Wright, K Keely Boyle, Thomas R Duquin, John K Crane
BACKGROUND: Many studies have noted an increase in the number of recognized cases of invasive infections due to Propionibacterium acnes, especially after shoulder replacement surgery. The increase in the number of recognized cases of P. acnes, a nonspore-forming, anaerobic, Gram-positive organism, appears due to both an increase in the number of shoulder operations being performed and more specimens being sent for anaerobic cultures. Nevertheless, the optimal surgical and antibiotic management of P...
2016: Infectious Diseases
Nina Mikirova, Ronald Hunnunghake, Ruth C Scimeca, Charles Chinshaw, Faryal Ali, Chris Brannon, Neil Riordan
BACKGROUND In neurofibromatosis type 1 (NF1) disease, the loss of the tumor suppressor function of the neurofibromin gene leads to proliferation of neural tumors. In children, the most frequently identified tumor is the optic pathway glioma. CASE REPORT We describe the case of a 5-year-old child who was diagnosed with NF1 and optic pathway tumor onset at the age of 14 months. Because of the tumor progression, chemotherapy with carboplatin and vincristine was prescribed at this early age and continued for one year...
October 24, 2016: American Journal of Case Reports
M A Tazón-Varela, H Alonso-Valle, P Muñoz-Cacho, J Gallo-Terán, X Piris-García, L A Pérez-Mier
INTRODUCTION: Knowing the most common microorganisms in our environment can help us to make proper empirical treatment decisions. The aim is to identify those microorganisms causing community-acquired pneumonia. PATIENTS AND METHODS: An observational, descriptive and prospective study was conducted, including patients over 14 years with a clinical and radiographic diagnosis of community-acquired pneumonia during a 383 consecutive day period. A record was made of sociodemographic variables, personal history, prognostic severity scales, progress, and pathogenic agents...
October 20, 2016: Semergen
Eugenia Bertona, Adriana N De Paulis, Miguel A Gutiérrez, Victoria Santa María, Carlos A Vay, Silvia C Predari
Dermabacter hominis species is constituted by Gram-positive facultative anaerobic coryneform rods being part of the resident microbiota human skin, and exceptionally associated to infections in immunocompromised or severely debilitated patients. An immunocompetent young adult woman with a neck sebaceous cyst infected by D. hominis as unique etiologic agent is presented. Phenotypic identification of the causative agent was achieved through simple tests, based on the originally scheme proposed by Funke and Bernard, and feasible to be performed in a hospital Microbiology Laboratory...
October 20, 2016: Revista Argentina de Microbiología
Shrikant S Maktedar, Shantilal S Mehetre, Gopal Avashthi, Man Singh
The rapid, robust, scalable and non-hazardous sonochemical approach for in situ reduction and direct functionalization of graphene oxide has been developed for non-toxic biomedical applications. The graphene oxide (GrO) was directly functionalized with tryptamine (TA) without using any hazardous acylating and coupling reagents. The reaction was completed within 20min. An impact of ultrasound was inferred for a direct functionalization with other conventional methods. The evolved electronic states were confirmed with near edge X-ray absorption fine structure (NEXAFS)...
January 2017: Ultrasonics Sonochemistry
Grazia T Micali, Björn A Espedido, Neville Firth, Slade O Jensen
No abstract text is available yet for this article.
February 2016: Pathology
Worawut Choeyprasert, Suradej Hongeng, Usanarat Anurathapan, Samart Pakakasama
Bacteremia during neutropenic episodes is a cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (HSCT). We have used oral ciprofloxacin and penicillin V, from the start of the conditioning regimen until engraftment, for the prophylaxis of bacterial infection. The objective of this study was to retrospectively analyze the prevalence of and risk factors for breakthrough bacteremia during neutropenic episodes in autologous and allogeneic HSCT patients. There were 215 patients enrolled, with a median age of 8...
October 22, 2016: International Journal of Hematology
Changcheng Zheng, Baolin Tang, Xiaoyu Zhu, Xuhan Zhang, Lei Zhang, Liangquan Geng, Huilan Liu, Zimin Sun
The aim of this study is to investigate the impact of pre-engraftment bloodstream infections (BSIs) on the outcomes in acute leukemia patients undergoing myeloablative cord blood transplantation (CBT). A total of 226 acute leukemia patients who received unrelated CBT were enrolled in this study, and all these patients received an intensified myeloablative conditioning without ATG. Pre-engraftment BSIs occurred in 72 patients (31.9 %), and the median time of onset was 4.5 days after cord blood infusion, BSIs of gram-negative bacilli, and gram-positive cocci comprised of 63...
October 22, 2016: Annals of Hematology
E Carneiro, A B Parolin, C Wichnieski, E A R Rosa, U X Silva Neto, V P D Westphalen, L F Fariniuk, A C B R Johann
OBJECTIVE: The study aimed to verify the potential correlation between the detected amount of gram-negative bacteria and the radiographic sizes of the lesions in patients with symptomatic and asymptomatic apical periodontitis. Furthermore, to evaluate whether the expression of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) and the RANKL/OPG ratio are differentially regulated in both groups. DESIGN: Twenty patients with periapical lesions were divided into two groups: symptomatic (SYM) n=10 and asymptomatic (ASYM) n=10...
October 15, 2016: Archives of Oral Biology
Zeba Nasir, Mohammad Shakir, Rizwan Wahab, Mohd Shoeb, Parvez Alam, Rizwan Hasan Khan, Mohammad Mobin, Lutfullah
Sn1-xCoxO2 (x=0.00, 0.01, 0.03, 0.05) nanoparticles (NPs) of average size ∼30-40nm were synthesized by co-precipitation method. The interaction of Co doped SnO2 NPs with human serum albumin (HSA) and their photocatalytic and antimicrobial properties were studied. The structural analysis and morphology of Co doped SnO2 NPs were analysed via X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FT-IR)...
October 19, 2016: International Journal of Biological Macromolecules
Anastasia V Nuzhdina, Alexey S Morozov, Maria N Kopitsyna, Elena N Strukova, Daria S Shlykova, Ivan V Bessonov, Elena S Lobakova
Novel quaternized polyethyleneimine and cross-linked polyethyleneimine derivatives have been synthesized using both traditional and microwave-assisted techniques to create antimicrobial coatings, with octyl, dodecyl, or hexadecyl bromides as alkylating agent and various bifunctional electrophiles as cross-linkers. Quaternization has been performed using methyl iodide or dimethyl sulfate; it has been shown that methyl iodide has no advantages over dimethyl sulfate. Antimicrobial activity of the polymers against Gram-positive (S...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
N Didbaridze, N Lomidze, T Abuladze, G Qiliptari, T Didbaridze, I Gvasalia, Z Mkervalishvili, N Gogokhia
Anaerobic clostridial infection is the most severe form of paraproctitis. The incubation period is very short, from 3 to 6 hours, sometimes lasting for 1-2 days. Clostridial infection spreads rapidly and induces gas gangrene, causes destruction of cells and other intermediate substances, and impedes blood circulation. This paper presents a case study of an extremely severe form of anaerobic infection with spontaneous gas gangrene, cellulitis, fasciomyositic necrosis, severe intoxication and septic shock on the abdominal front and lateral surfaces...
September 2016: Georgian Medical News
T Didbaridze, L Saginashvili, L Akhmeteli, B Iremashvili, N Gogokhia
This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep...
September 2016: Georgian Medical News
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