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https://www.readbyqxmd.com/read/29040928/calorimetric-and-spectroscopic-studies-of-the-interaction-between-zidovudine-and-human-serum-albumin
#1
Adrian Pîrnău, Mihaela Mic, Silvia Neamţu, Călin G Floare, Mircea Bogdan
A quantitative analysis of the interaction between zidovudine (AZT) and human serum albumin (HSA) was achieved using Isothermal titration calorimetry (ITC) in combination with fluorescence and (1)H NMR spectroscopy. ITC directly measure the heat during a biomolecular binding event and gave us thermodynamic parameters and the characteristic association constant. By fluorescence quenching, the binding parameters of AZT-HSA interaction was determined and location to binding site I of HSA was confirmed. Via T1 NMR selective relaxation time measurements the drug-protein binding extent was evaluated as dissociation constants Kd and the involvement of azido moiety of zidovudine in molecular complex formation was put in evidence...
October 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29040778/multimodality-management-of-recurrent-skull-base-chordomas-factors-impacting-tumor-control-and-disease-specific-survival
#2
Shaan M Raza, Diana Bell, Jacob L Freeman, David R Grosshans, Gregory N Fuller, Franco DeMonte
BACKGROUND: Limited data exist to guide the management of recurrent chordomas arising in the skull base. OBJECTIVE: To determine factors affecting tumor control rates and disease-specific survival (DSS) in recurrent disease. METHODS: A retrospective review was performed of 29 patients with 55 recurrences treated at our institution. Tumor and treatment factors were assessed for impact on freedom from progression (FFP; primary outcome) and DSS (secondary outcome)...
October 10, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29040729/csra-maximizes-expression-of-the-acrab-multidrug-resistance-transporter
#3
Vito Ricci, Victoria Attah, Tim Overton, David C Grainger, Laura J V Piddock
Carbon Storage Regulator A (CsrA) is an RNA binding protein that acts as a global regulator of diverse genes. Using a combination of genetics and biochemistry we show that CsrA binds directly to the 5' end of the transcript encoding AcrAB. Deletion of csrA or mutagenesis of the CsrA binding sites reduced production of both AcrA and AcrB. Nucleotide substitutions at the 5' UTR of acrA mRNA that could potentially weaken the inhibitory RNA secondary structure, allow for more efficient translation of the AcrAB proteins...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040720/genome-wide-prediction-of-minor-groove-electrostatic-potential-enables-biophysical-modeling-of-protein-dna-binding
#4
Tsu-Pei Chiu, Satyanarayan Rao, Richard S Mann, Barry Honig, Remo Rohs
Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (shape readout). The underlying mechanism of DNA shape readout involves the correlation between minor-groove width and electrostatic potential (EP). To probe this biophysical effect directly, rather than using minor-groove width as an indirect measure for shape readout, we developed a methodology, DNAphi, for predicting EP in the minor groove and confirmed the direct role of EP in protein-DNA binding using massive sequencing data...
October 11, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040719/the-effect-of-insecticidal-stress-on-reproductive-output-of-susceptible-and-field-strains-of-aedes-aegypti-diptera-culicidae
#5
Lee-Jin Bong, Wu-Chun Tu, Kok-Boon Neoh, Chin-Gi Huang, Rou-Xing Ting
The phenomenon of high egg reproduction when mortality risk rises is common in mosquitoes. However, the phenomenon may vary between insecticide susceptible and field-collected strains, due to the latter's decreased energy allocation in reproduction in the presence of insecticide resistance. In this study, we evaluated the effect of chlorpyrifos (CP) and temephos (TP) exposure on the oviposition and survival of Aedes aegypti (Linnaeus) (Diptera: Culicidae) using a susceptible strain (KHsm) and two field strains (KHly and TNnorth)...
October 10, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29040689/regulatory-dynamics-in-the-ternary-dnaa-complex-for-initiation-of-chromosomal-replication-in-escherichia-coli
#6
Yukari Sakiyama, Kazutoshi Kasho, Yasunori Noguchi, Hironori Kawakami, Tsutomu Katayama
In Escherichia coli, the level of the ATP-DnaA initiator is increased temporarily at the time of replication initiation. The replication origin, oriC, contains a duplex-unwinding element (DUE) flanking a DnaA-oligomerization region (DOR), which includes twelve DnaA-binding sites (DnaA boxes) and the DNA-bending protein IHF-binding site (IBS). Although complexes of IHF and ATP-DnaA assembly on the DOR unwind the DUE, the configuration of the crucial nucleoprotein complexes remains elusive. To resolve this, we analyzed individual DnaA protomers in the complex and here demonstrate that the DUE-DnaA-box-R1-IBS-DnaA-box-R5M region is essential for DUE unwinding...
October 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040658/fire-does-not-strongly-affect-genetic-diversity-or-structure-of-a-common-treefrog-in-the-endangered-florida-scrub
#7
J M Robertson, S W Fitzpatrick, B B Rothermel, L M Chan
Fire regimes influence natural populations of organisms in diverse ways, via direct effects on population dynamics as well as indirect effects on habitat and ecosystem processes. Although many amphibian species have evolved to persist in fire-dependent ecosystems, the effects of fire on the genetic diversity of amphibian populations remain relatively unexplored. We examined how different aspects of fire history relate to population genetic diversity and structure of an abundant anuran, Hyla femoralis, in a large, intact area of Florida scrub containing hundreds of seasonally inundated ponds...
October 11, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/29040534/macrophages-escape-klotho-gene-silencing-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy-and-promote-muscle-growth-and-increase-satellite-cell-numbers-through-a-klotho-mediated-pathway
#8
Michelle Wehling-Henricks, Steven S Welc, Guiseppina Samengo, Chiara Rinaldi, Catherine Lindsey, Ying Wang, Jeongyoon Lee, Makoto Kuro-O, James G Tidball
Duchenne muscular dystrophy (DMD) is a muscle wasting disease in which inflammation influences the severity of pathology. We found that the onset of muscle inflammation in the mdx mouse model of DMD coincides with large increases in expression of pro-inflammatory cytokines [tumor necrosis factor-α (TNFα); interferon gamma (IFNγ)] and dramatic reductions of the pro-myogenic protein Klotho in muscle cells and large increases of Klotho in pro-regenerative, CD206+ macrophages. Furthermore, TNFα and IFNγ treatments reduced Klotho in muscle cells and increased Klotho in macrophages...
October 12, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29040189/the-patient-and-observer-scar-assessment-scale-to-evaluate-the-cosmetic-outcomes-of-the-robotic-single-site-hysterectomy-in-endometrial-cancer
#9
Giacomo Corrado, Gloria Calagna, Giuseppe Cutillo, Salvatore Insinga, Emanuela Mancini, Ermelinda Baiocco, Ashanti Zampa, Arabella Bufalo, Antonio Perino, Enrico Vizza
OBJECTIVE: The objective of this study was to evaluate the cosmetic outcome of robotic single-site hysterectomy (RSSH) in early-stage endometrial cancer. METHODS: We prospectively collected patient demographics, operative times, complications, pathologic results, and length of stay on all patients who underwent RSSH for early-stage endometrial cancer. The Patient and Observer Scar Assessment Scale (POSAS) was used for the evaluation of the cosmetic outcome. RESULTS: Forty-five patients were included in our study from January 2012 to October 2015...
October 17, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/29039943/synthesis-and-structural-electrical-and-magnetic-properties-of-new-iron-aluminum-alluaudite-phases-%C3%AE-na2ni2m-po4-3-m-fe-and-al
#10
Douha Harbaoui, Moustafa M S Sanad, Cécile Rossignol, El Kebir Hlil, Noureddine Amdouni, Saïd Obbade
Herein we report the studies of different physical properties (structural, magnetic, thermal, morphologic, electrical, and electrochemical) of two new allotropic β-Na2Ni2M(PO4)3 (NNMP) phosphates, with M = Fe and Al. Pure orthorhombic single-phase powders were prepared under air, using an autocombustion synthesis method. They crystallize in the orthorhombic Imma space group with similar unit cell parameters [a = 10.1592(2), b = 13.0321(3), c = 6.4864(2) Å] and [a = 10.3993(1), b = 13.1966(1), c = 6.4955(1) Å] for β-Na2Ni2M(PO4)3 (NNAP) and β-Na2Ni2Fe(PO4)3 (NNFP), respectively...
October 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29039928/using-hyperfine-epr-spectroscopy-to-define-the-proton-coupled-electron-transfer-reaction-at-fe-s-cluster-n2-in-respiratory-complex-i
#11
Nolwenn Le Breton, John J Wright, Andrew J Y Jones, Enrico Salvadori, Hannah R Bridges, Judy Hirst, Maxie M Roessler
Energy-transducing respiratory complex I (NADH:ubiquinone oxidoreductase) is one of the largest and most complicated enzymes in mammalian cells. Here, we used hyperfine EPR spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of a single proton-coupled electron transfer reaction at one of eight iron-sulfur clusters in complex I, [4Fe-4S] cluster N2. N2 is the terminal cluster of the enzyme's intramolecular electron-transfer chain and the electron donor to ubiquinone. Because of its position and pH dependent reduction potential, N2 has long been considered a candidate for the elusive 'energy-coupling' site in complex I, at which energy generated by the redox reaction is used to initiate proton translocation...
October 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29036977/-safety-and-feasibility-of-sheathless-transfemoral-aortic-valve-implantation
#12
H J Pei, S Y Teng, T Luo, Y J Wu, Y J Yang, S B Qiao, B Xu, R L Gao
Objective: To investigate the safety and feasibility of sheathless transfemoral aortic valve replacement (TAVR). Methods: In this prospective study, we enrolled 23 patients with severe aortic stenosis (AS) who were inoperable or at high-risk for surgical aortic valve replacement operation in Fuwai hospital From September 2012 to June 2015. Multislice spiral CT and angiography of femoral artery showed that all patients had minimal femoral artery diameters (<6.5 mm) and severe calcification which was not suitable for transfemoral TAVR through sheath...
September 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29036670/catalytic-and-structural-properties-of-pheophytinase-the-phytol-esterase-involved-in-chlorophyll-breakdown
#13
Luzia Guyer, Kathrin Salinger, Undine Krügel, Stefan Hörtensteiner
During leaf senescence and fruit ripening, chlorophyll is degraded in a multistep pathway into linear tetrapyrroles called phyllobilins. A key feature of chlorophyll breakdown is the removal of the hydrophobic phytol chain that renders phyllobilins water soluble, an important prerequisite for their ultimate storage in the vacuole of senescent cells. Chlorophyllases had been considered for more than a century to catalyze dephytylation in vivo; however, this was recently refuted. Instead, pheophytinase was discovered as a genuine in vivo phytol hydrolase...
September 23, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29036662/parp2-controls-double-strand-break-repair-pathway-choice-by-limiting-53bp1-accumulation-at-dna-damage-sites-and-promoting-end-resection
#14
Alexis Fouquin, Josée Guirouilh-Barbat, Bernard Lopez, Janet Hall, Mounira Amor-Guéret, Vincent Pennaneach
Double strand breaks (DSBs) are one of the most toxic lesions to cells. DSB repair by the canonical non-homologous end-joining (C-EJ) pathway involves minor, if any, processing of the broken DNA-ends, whereas the initiation of DNA resection channels the broken-ends toward DNA repair pathways using various lengths of homology. Mechanisms that control the resection initiation are thus central to the regulation to the choice of DSB repair pathway. Therefore, understanding the mechanisms which regulate the initiation of DNA end-resection is of prime importance...
October 3, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036440/analysis-and-prediction-of-protein-folding-energy-changes-upon-mutation-by-element-specific-persistent-homology
#15
Zixuan Cang, Guo-Wei Wei
Motivation: Site directed mutagenesis is widely used to understand the structure and function of biomolecules. Computational prediction of mutation impacts on protein stability offers a fast, economical and potentially accurate alternative to laboratory mutagenesis. Most existing methods rely on geometric descriptions, this work introduces a topology based approach to provide an entirely new representation of mutation induced protein stability changes that could not be obtained from conventional techniques...
July 14, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29036434/novel-mechanisms-of-eif2b-action-and-regulation-by-eif2%C3%AE-phosphorylation
#16
Andrew M Bogorad, Kai Ying Lin, Assen Marintchev
Eukaryotic translation initiation factor 2 (eIF2) is a heterotrimeric GTPase, which plays a critical role in protein synthesis regulation. eIF2-GTP binds Met-tRNAi to form the eIF2-GTP•Met-tRNAi ternary complex (TC), which is recruited to the 40S ribosomal subunit. Following GTP hydrolysis, eIF2-GDP is recycled back to TC by its guanine nucleotide exchange factor (GEF), eIF2B. Phosphorylation of the eIF2α subunit in response to various cellular stresses converts eIF2 into a competitive inhibitor of eIF2B, which triggers the integrated stress response (ISR)...
September 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29035996/radioguided-occult-lesion-localization-technical-procedures-and-clinical-applications
#17
Gianpiero Manca, Sara Mazzarri, Domenico Rubello, Elisa Tardelli, Roberto C Delgado-Bolton, Francesco Giammarile, Manuela Roncella, Duccio Volterrani, Patrick M Colletti
PURPOSE: Regarding radioguided surgery, the concept of "radioguided occult lesion localization" (ROLL) is based on both preoperative interventional imaging and intraoperative radioguided detection of a clinically occult neoplastic lesion. METHODS: This methodology consists in the direct administration into the lesion of Tc-macroaggregated human albumin formed by relatively large particles retained at the injection site, which direct radioguided excisional biopsy...
October 13, 2017: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/29035989/-it-doesn-t-make-sense-for-us-not-to-have-one-understanding-reasons-why-community-sports-organizations-chose-to-participate-in-a-funded-automated-external-defibrillator-program
#18
Lauren V Fortington, Sheree Bekker, Damian Morgan, Caroline F Finch
OBJECTIVE: Implementation of automated external defibrillators (AEDs) in community sports settings is an important component of emergency medical planning. This study aimed to understand motivations for why sports organizations participated in a government-funded program that provided AEDs and associated first-aid training. DESIGN: Face-to-face interviews. SETTING: Community sports organizations in Victoria, Australia. PARTICIPANTS: Representatives from 14 organizations who participated in a government-funded AED program...
October 10, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/29035536/hdac8-substrates-identified-by-genetically-encoded-active-site-photocrosslinking
#19
Jeffrey E Lopez, Sarah E Haynes, Jaimeen D Majmudar, Brent R Martin, Carol A Fierke
The histone deacetylase family comprises 18 enzymes that catalyze deacetylation of acetylated lysine residues, however, the specificity and substrate profile of each enzyme remains largely unknown. Due to transient enzyme-substrate interactions, conventional co-immunoprecipitation methods frequently fail to identify enzyme-specific substrates. Additionally, compensatory mechanisms often limit the ability of knockdown or chemical inhibition studies to achieve significant fold-changes observed by acetylation proteomics methods...
October 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29035509/positron-emission-tomography-assessment-of-the-intranasal-delivery-route-for-orexin-a
#20
Genevieve C Van de Bittner, Kyle C Van de Bittner, Hsiao-Ying Wey, Wayne Rowe, Ramalinga Dharanipragada, Xiaoyou Ying, William Hurst, Andrew Giovanni, Kim Alving, Anurag Gupta, John Hoekman, Jacob M Hooker
Intranasal drug delivery is a non-invasive drug delivery route that can enhance systemic delivery of therapeutics with poor oral bioavailability by exploiting the rich microvasculature within the nasal cavity. The intranasal delivery route has also been targeted as a method for improved brain uptake of neurotherapeutics, with a goal of harnessing putative, direct nose-to-brain pathways. Studies in rodents, non-human primates and humans have pointed to the efficacy of intranasally delivered neurotherapeutics, while radiolabeling studies have analyzed brain uptake following intranasal administration...
October 16, 2017: ACS Chemical Neuroscience
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